Rw7G016100

Subtilisin-like serine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000302
Physical Location & Seq
Reverse (-)
16462231 .. 16465210
2980 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G016100.1

Sequence Viewer

Length: 2016 bp
ATGGAGAACAAAAGGAGTGTAGCAATTCGGTTTACTTATCTTCTTGGCCTCATTTTCATGTTTTATAGCATGCGAACTTATAACGTTTGGGTACGAAAGCCCGCAGACGAATTTTCTGCACTACATTCTCAAGATTTGGAGAGTTGGTATCAGACATTTCTGCCGGAAAGTACTATTGCAAGCTCAAACAAGCCCATCTATCGGCGAATGGTTCACTCATACCGCAATGTGGCAACTGGCTTTGCAGCGAAACTGACACCCGAGGAAGTGAAAGCAATGGAAAAGAAAGATGGGTTCGTGCATGCTCATCCAGATAGGCTTCTGCCTTTGCAAACTACTCATAGGACTAATTGCGGGGAAGGTGTGATCATTGGAGTTTTGGATACTGGAATATCTCCAGATCATCCCTCATTTAGTGATGATGGATGCAAGTTCAATGGGACAGTGTGTAACAACAAGCTTATTGGTGCAAGAAATTTCCAAGGTGGGAAAGAAACAAAAGGAGTCTCTCCATTTGATGAAGAAGGCCATGGCACTCACACCTCCAGCACAGCTGCCGGAAACTTTGTGAAAGGTGCTAGCGTGTTTGGAATGGCAAATGGCACAGCCTCTGGCATTGCACCTTATGCTTACTTAGCCATCTACAAGGTCTGCATCGAGGCAGGTTGTGCCGAAAGTGACATGCTAGCAGCTCTTGACACTGCTGTTGAGGACGGCGTGGATGTGCTCTCACTGTCCATTAGTGATTCCTCAATTCCTTTCCATCAAGATGGGATTGCTATCGGCGCGTTTGGAGCAATTCAAAAGGGAATTTTTGTCAGCTGTTCAGCTGGAAACTCTGGCCCTTTCTTTAGTTCCATGTCGAATGAGGCCCCTTGGATTCTCACCCTTGCTATGCTCGGTCCAGGTGACAGAAAAATTCCGGCGCTGGCTATGCTCGATCCTAAAATTTTTAGCACTTCACCATTGCTACCTCTTGTTTATCCAGGTGCAAATCCCAATAACCAAACTGAGTTCTGTCCCTCGGGATCTCTTAGAGGTGGAGGAGTGGGAAGAATTGCCGAAGGAGAAGAAGTGAAGAGAGCAGGAGGTGCTGCCATGATTCTAGTGAACCAAATCACGGATGACTATAGCACCTTAGCTGATGCTCATGTACTTCCAGCAACAAATGTTGGTTTTCAAGCAGGTTTGAGTATCAAAACCTATATTAACTCAACAACAACACCCATAGCCAAAGTCTATTCCCCACGTACTAAAATAGGAGATGCACCTTGCTCCGATGGTTGCTTCTTTCTCTGTTTCTCATCCAGAGGACCGAGCATAGCCAGCCCCGGAATTTTGAAACCTGACATCATTGGTCCTGGTGTTAACATCCTCGTTGCATGGCCTGTTTCGGTGGATAATCATGATTTGAATTCAGGTACCTCAATGTCATGCCCGCACCTAAGCGGCATTGCAGCCTTGATCAAAAGTTCACACCCAGACTGGTCACCTGCTGCCATCAAATCCGCCATTATGACAACTGCTGATCTGCTAAACCTCAAAGGCACACCCAGTGGCGGAACCACTATTCGATCAACTTCCCCAGCAGACATCTATAGCGCGGGCTCAGGCCATGTCAACCCTTCAAAAGCCAATGACCTGGCGCTCATCTATGACACAAAACCAGAAGATTACATTCCTTACTTGTGTGGTCTGAACTACACAGACAATCAAATAGAGATAATCACCTGGCCAACAAAGAAGAAGCTAAACTATCCTTCATTTTCTATCACAATAGGGTCCTATCAAACTCAGAATTACACCAAAACCGTGACAAATGTTGGCCCGGCTAATTTAACCTACAAATTGGAGATTTTGGAACCTCAGAAATGGGGTGTGAGTGTGAACATGAGTGTGACTCCTTCAGTGCTTACATTCACACAGGTCAACCAGACCATTACATACAATGTGGAGTTTTCCACACAACTGGCTGGGCAAGATGGTTCATTTTCACAGGGATTCATGGGTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

671

Amino Acids

71.68

Weight (kDa)

5.98

Isoelectric Point (pI)

31.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 42 - 110 3.6e-09 Peptidase inhibitor I9
Peptidase_S8 PF00082 119 - 510 3.8e-39 Subtilase family
fn3_6 PF17766 583 - 668 9.1e-16 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 81
AarI CACCTGC 1 cut(s) 1501
Acc36I ACCTGC 3 cut(s) 653, 1175, 1501
Acc65I GGTACC 1 cut(s) 1421
AccB1I GGYRCC 1 cut(s) 1421
AccII CGCG 2 cut(s) 788, 1604
AciI CCGC 8 cut(s) 102, 223, 354, 1439, 1449, 1509, 1560, 1604
AclI AACGTT 1 cut(s) 84
AclWI GGATC 2 cut(s) 935, 1036
AcoI YGGCCR 1 cut(s) 1733
AcsI RAATTY 7 cut(s) 110, 475, 810, 918, 948, 1335, 1414
AcuI CTGAAG 1 cut(s) 1890
AdeI CACNNNGTG 1 cut(s) 1556
AfaI GTAC 5 cut(s) 93, 172, 1155, 1252, 1423
AfiI CCNNNNNNNGG 4 cut(s) 201, 229, 1120, 1559
AgsI TTSAA 6 cut(s) 436, 803, 1181, 1342, 1414, 1629
AjnI CCWGG 5 cut(s) 904, 985, 1360, 1641, 1730
AluBI AGCT 8 cut(s) 183, 460, 554, 692, 822, 830, 1142, 1750
AluI AGCT 8 cut(s) 183, 460, 554, 692, 822, 830, 1142, 1750
Alw21I GWGCWC 1 cut(s) 729
Alw26I GTCTC 1 cut(s) 511
AlwI GGATC 2 cut(s) 935, 1036
AlwNI CAGNNNCTG 1 cut(s) 611
Ama87I CYCGRG 2 cut(s) 260, 1024
AoxI GGCC 8 cut(s) 46, 526, 841, 870, 1385, 1612, 1733, 1825
ApeKI GCWGC 6 cut(s) 245, 554, 689, 1094, 1457, 1496
ApoI RAATTY 7 cut(s) 110, 475, 810, 918, 948, 1335, 1414
Asp718I GGTACC 1 cut(s) 1421
AspLEI GCGC 4 cut(s) 788, 928, 1604, 1648
AspS9I GGNCC 7 cut(s) 842, 871, 902, 1313, 1358, 1782, 1826
AsuC2I CCSGG 2 cut(s) 1332, 1829
AsuHPI GGTGA 5 cut(s) 877, 920, 954, 1482, 1720
AsuNHI GCTAGC 2 cut(s) 578, 685
AvaI CYCGRG 2 cut(s) 260, 1024
AvaII GGWCC 4 cut(s) 902, 1313, 1358, 1782
BalI TGGCCA 1 cut(s) 1735
BanI GGYRCC 1 cut(s) 1421
BanII GRGCYC 1 cut(s) 1610
Bbv12I GWGCWC 1 cut(s) 729
BbvI GCAGC 6 cut(s) 257, 541, 701, 1081, 1469, 1483
BccI CCATC 9 cut(s) 203, 284, 416, 647, 764, 771, 1274, 1508, 1976
BceAI ACGGC 1 cut(s) 730
BcgI CGANNNNNNTGC 2 cut(s) 98, 132
BciT130I CCWGG 5 cut(s) 906, 987, 1362, 1643, 1732
BciVI GTATCC 1 cut(s) 376
BclI TGATCA 2 cut(s) 366, 1464
BcnI CCSGG 2 cut(s) 1332, 1829
BcoDI GTCTC 1 cut(s) 511
BfaI CTAG 3 cut(s) 579, 686, 1106
BfmI CTRYAG 2 cut(s) 1129, 1597
BfoI RGCGCY 2 cut(s) 929, 1649
BfuAI ACCTGC 3 cut(s) 653, 1175, 1501
BfuI GTATCC 1 cut(s) 376
BisI GCNGC 7 cut(s) 246, 555, 690, 1095, 1450, 1458, 1497
BlsI GCNGC 7 cut(s) 247, 556, 691, 1096, 1451, 1459, 1498
BmcAI AGTACT 1 cut(s) 172
Bme1390I CCNGG 7 cut(s) 906, 987, 1332, 1362, 1643, 1732, 1829
Bme18I GGWCC 4 cut(s) 902, 1313, 1358, 1782
BmeT110I CYCGRG 2 cut(s) 260, 1024
BmgT120I GGNCC 7 cut(s) 842, 871, 902, 1313, 1358, 1782, 1826
BmiI GGNNCC 5 cut(s) 873, 1423, 1564, 1783, 1863
BmrFI CCNGG 7 cut(s) 906, 987, 1332, 1362, 1643, 1732, 1829
BmrI ACTGGG 1 cut(s) 1548
BmsI GCATC 4 cut(s) 416, 663, 1135, 1255
BmtI GCTAGC 2 cut(s) 582, 689
BmuI ACTGGG 1 cut(s) 1548
BplI GAGNNNNNCTC 2 cut(s) 1885, 1917
BpmI CTGGAG 2 cut(s) 381, 529
Bpu10I CCTNAGC 3 cut(s) 1138, 1445, 1609
BpuEI CTTGAG 1 cut(s) 114
BpuMI CCSGG 2 cut(s) 1332, 1829
BsaAI YACGTR 1 cut(s) 1250
BsaBI GATNNNNATC 1 cut(s) 779
BsaJI CCNNGG 6 cut(s) 261, 481, 529, 875, 1023, 1330
BsaXI ACNNNNNCTCC 4 cut(s) 131, 161, 1059, 1089
Bsc4I CCNNNNNNNGG 4 cut(s) 201, 229, 1120, 1559
Bse1I ACTGG 5 cut(s) 241, 391, 1490, 1554, 1974
Bse3DI GCAATG 5 cut(s) 232, 282, 615, 965, 1452
Bse8I GATNNNNATC 1 cut(s) 779
BseBI CCWGG 5 cut(s) 906, 987, 1362, 1643, 1732
BseDI CCNNGG 6 cut(s) 261, 481, 529, 875, 1023, 1330
BseGI GGATG 7 cut(s) 307, 403, 431, 727, 1129, 1304, 1371
BseJI GATNNNNATC 1 cut(s) 779
BseLI CCNNNNNNNGG 4 cut(s) 201, 229, 1120, 1559
BseMI GCAATG 5 cut(s) 232, 282, 615, 965, 1452
BseMII CTCAG 4 cut(s) 1002, 1623, 1808, 1880
BseNI ACTGG 5 cut(s) 241, 391, 1490, 1554, 1974
BseRI GAGGAG 1 cut(s) 1059
BseXI GCAGC 6 cut(s) 257, 541, 701, 1081, 1469, 1483
BseYI CCCAGC 2 cut(s) 1585, 1973
BsgI GTGCAG 1 cut(s) 102
Bsh1236I CGCG 2 cut(s) 788, 1604
BshFI GGCC 8 cut(s) 48, 528, 843, 872, 1387, 1614, 1735, 1827
BshNI GGYRCC 1 cut(s) 1421
BsiHKAI GWGCWC 1 cut(s) 729
BsiHKCI CYCGRG 2 cut(s) 260, 1024
BsiSI CCGG 5 cut(s) 164, 558, 923, 1332, 1829
BslFI GGGAC 2 cut(s) 454, 1005
BslI CCNNNNNNNGG 4 cut(s) 201, 229, 1120, 1559
BsmAI GTCTC 1 cut(s) 511
BsmFI GGGAC 2 cut(s) 454, 1005
BsnI GGCC 8 cut(s) 48, 528, 843, 872, 1387, 1614, 1735, 1827
BsoBI CYCGRG 2 cut(s) 260, 1024
Bsp1286I GDGCHC 2 cut(s) 729, 1610
Bsp143I GATC 7 cut(s) 366, 400, 940, 1028, 1464, 1528, 1574
Bsp19I CCATGG 1 cut(s) 529
BspACI CCGC 8 cut(s) 102, 223, 354, 1439, 1449, 1509, 1560, 1604
BspANI GGCC 8 cut(s) 48, 528, 843, 872, 1387, 1614, 1735, 1827
BspCNI CTCAG 4 cut(s) 1003, 1622, 1807, 1879
BspFNI CGCG 2 cut(s) 788, 1604
BspHI TCATGA 1 cut(s) 1405
BspLI GGNNCC 5 cut(s) 873, 1423, 1564, 1783, 1863
BspMI ACCTGC 3 cut(s) 653, 1175, 1501
BspOI GCTAGC 2 cut(s) 582, 689
BspPI GGATC 2 cut(s) 935, 1036
BspT107I GGYRCC 1 cut(s) 1421
BsrDI GCAATG 5 cut(s) 232, 282, 615, 965, 1452
BsrI ACTGG 5 cut(s) 241, 391, 1490, 1554, 1974
BssECI CCNNGG 6 cut(s) 261, 481, 529, 875, 1023, 1330
BssMI GATC 7 cut(s) 366, 400, 940, 1028, 1464, 1528, 1574
BssT1I CCWWGG 3 cut(s) 481, 529, 875
Bst2UI CCWGG 5 cut(s) 906, 987, 1362, 1643, 1732
Bst4CI ACNGT 3 cut(s) 445, 735, 1813
Bst6I CTCTTC 1 cut(s) 1073
BstAPI GCANNNNNTGC 3 cut(s) 626, 668, 1091
BstBAI YACGTR 1 cut(s) 1250
BstDEI CTNAG 8 cut(s) 634, 1011, 1034, 1138, 1445, 1609, 1794, 1866
BstDSI CCRYGG 1 cut(s) 529
BstEII GGTNACC 1 cut(s) 1488
BstF5I GGATG 7 cut(s) 307, 403, 431, 727, 1129, 1304, 1371
BstFNI CGCG 2 cut(s) 788, 1604
BstH2I RGCGCY 2 cut(s) 929, 1649
BstHHI GCGC 4 cut(s) 788, 928, 1604, 1648
BstKTI GATC 7 cut(s) 369, 403, 943, 1031, 1467, 1531, 1577
BstMAI GTCTC 1 cut(s) 511
BstMBI GATC 7 cut(s) 366, 400, 940, 1028, 1464, 1528, 1574
BstMWI GCNNNNNNNGC 8 cut(s) 626, 635, 668, 785, 794, 934, 1091, 1326
BstNI CCWGG 5 cut(s) 906, 987, 1362, 1643, 1732
BstNSI RCATGY 3 cut(s) 73, 305, 685
BstPI GGTNACC 1 cut(s) 1488
BstSCI CCNGG 7 cut(s) 904, 985, 1330, 1360, 1641, 1730, 1827
BstSFI CTRYAG 2 cut(s) 1129, 1597
BstUI CGCG 2 cut(s) 788, 1604
BstV1I GCAGC 6 cut(s) 257, 541, 701, 1081, 1469, 1483
BstX2I RGATCY 1 cut(s) 1028
BstXI CCANNNNNNTGG 3 cut(s) 770, 1642, 1969
BstYI RGATCY 1 cut(s) 1028
BsuI GTATCC 1 cut(s) 376
BsuRI GGCC 8 cut(s) 48, 528, 843, 872, 1387, 1614, 1735, 1827
BtgI CCRYGG 1 cut(s) 529
BtsCI GGATG 7 cut(s) 307, 403, 431, 727, 1129, 1304, 1371
BtsI GCAGTG 1 cut(s) 699
BtsIMutI CAGTG 5 cut(s) 450, 699, 731, 1561, 1914
BveI ACCTGC 3 cut(s) 653, 1175, 1501
CaiI CAGNNNCTG 1 cut(s) 611
CciI TCATGA 1 cut(s) 1405
CfoI GCGC 4 cut(s) 788, 928, 1604, 1648
Cfr13I GGNCC 7 cut(s) 842, 871, 902, 1313, 1358, 1782, 1826
Csp6I GTAC 5 cut(s) 92, 171, 1154, 1251, 1422
CviQI GTAC 5 cut(s) 92, 171, 1154, 1251, 1422
DdeI CTNAG 8 cut(s) 634, 1011, 1034, 1138, 1445, 1609, 1794, 1866
DpnI GATC 7 cut(s) 368, 402, 942, 1030, 1466, 1530, 1576
DpnII GATC 7 cut(s) 366, 400, 940, 1028, 1464, 1528, 1574
DraIII CACNNNGTG 1 cut(s) 1556
EaeI YGGCCR 1 cut(s) 1733
Eam1104I CTCTTC 1 cut(s) 1073
EarI CTCTTC 1 cut(s) 1073
EciI GGCGGA 2 cut(s) 1498, 1575
Eco130I CCWWGG 3 cut(s) 481, 529, 875
Eco24I GRGCYC 1 cut(s) 1610
Eco47I GGWCC 4 cut(s) 902, 1313, 1358, 1782
Eco57I CTGAAG 1 cut(s) 1890
Eco88I CYCGRG 2 cut(s) 260, 1024
Eco91I GGTNACC 1 cut(s) 1488
EcoO109I RGGNCCY 2 cut(s) 871, 1782
EcoO65I GGTNACC 1 cut(s) 1488
EcoRI GAATTC 1 cut(s) 1414
EcoRII CCWGG 5 cut(s) 904, 985, 1360, 1641, 1730
EcoT14I CCWWGG 3 cut(s) 481, 529, 875
EcoT38I GRGCYC 1 cut(s) 1610
ErhI CCWWGG 3 cut(s) 481, 529, 875
FaqI GGGAC 2 cut(s) 454, 1005
FauI CCCGC 4 cut(s) 109, 347, 1446, 1597
FbaI TGATCA 2 cut(s) 366, 1464
Fnu4HI GCNGC 7 cut(s) 246, 555, 690, 1095, 1450, 1458, 1497
FokI GGATG 7 cut(s) 294, 390, 438, 734, 1136, 1291, 1358
FriOI GRGCYC 1 cut(s) 1610
Fsp4HI GCNGC 7 cut(s) 246, 555, 690, 1095, 1450, 1458, 1497
FspBI CTAG 3 cut(s) 579, 686, 1106
GlaI GCGC 4 cut(s) 787, 927, 1603, 1647
GluI GCNGC 7 cut(s) 246, 555, 690, 1095, 1450, 1458, 1497
GsaI CCCAGC 2 cut(s) 1589, 1977
GsuI CTGGAG 2 cut(s) 381, 529
HaeII RGCGCY 2 cut(s) 929, 1649
HaeIII GGCC 8 cut(s) 48, 528, 843, 872, 1387, 1614, 1735, 1827
HapII CCGG 5 cut(s) 164, 558, 923, 1332, 1829
HhaI GCGC 4 cut(s) 788, 928, 1604, 1648
Hin6I GCGC 4 cut(s) 786, 926, 1602, 1646
HinP1I GCGC 4 cut(s) 786, 926, 1602, 1646
HincII GTYRAC 3 cut(s) 1369, 1621, 1930
HindII GTYRAC 3 cut(s) 1369, 1621, 1930
HindIII AAGCTT 1 cut(s) 458
HinfI GANTC 6 cut(s) 504, 746, 880, 1102, 1900, 2001
HpaI GTTAAC 1 cut(s) 1369
HpaII CCGG 5 cut(s) 164, 558, 923, 1332, 1829
HphI GGTGA 5 cut(s) 877, 920, 954, 1482, 1720
Hpy166II GTNNAC 8 cut(s) 33, 214, 1111, 1369, 1475, 1621, 1888, 1930
Hpy188I TCNGA 6 cut(s) 153, 1279, 1698, 1797, 1869, 2015
Hpy188III TCNNGA 8 cut(s) 131, 311, 398, 695, 767, 1026, 1308, 1406
Hpy8I GTNNAC 8 cut(s) 33, 214, 1111, 1369, 1475, 1621, 1888, 1930
HpyAV CCTTC 6 cut(s) 353, 518, 1058, 1635, 1770, 1914
HpyCH4III ACNGT 3 cut(s) 445, 735, 1813
HpyCH4IV ACGT 2 cut(s) 84, 1249
HpyF10VI GCNNNNNNNGC 8 cut(s) 626, 635, 668, 785, 794, 934, 1091, 1326
HpyF3I CTNAG 8 cut(s) 634, 1011, 1034, 1138, 1445, 1609, 1794, 1866
HpySE526I ACGT 2 cut(s) 84, 1249
HspAI GCGC 4 cut(s) 786, 926, 1602, 1646
KpnI GGTACC 1 cut(s) 1425
Ksp22I TGATCA 2 cut(s) 366, 1464
KspAI GTTAAC 1 cut(s) 1369
Kzo9I GATC 7 cut(s) 366, 400, 940, 1028, 1464, 1528, 1574
LmnI GCTCC 2 cut(s) 794, 1280
Lsp1109I GCAGC 6 cut(s) 257, 541, 701, 1081, 1469, 1483
LweI GCATC 4 cut(s) 416, 663, 1135, 1255
MaeI CTAG 3 cut(s) 579, 686, 1106
MaeII ACGT 2 cut(s) 84, 1249
MaeIII GTNAC 6 cut(s) 449, 677, 908, 1488, 1813, 1897
MalI GATC 7 cut(s) 368, 402, 942, 1030, 1466, 1530, 1576
MboI GATC 7 cut(s) 366, 400, 940, 1028, 1464, 1528, 1574
MboII GAAGA 7 cut(s) 32, 533, 1065, 1082, 1090, 1682, 1756
MflI RGATCY 1 cut(s) 1028
MhlI GDGCHC 2 cut(s) 729, 1610
MlsI TGGCCA 1 cut(s) 1735
MluNI TGGCCA 1 cut(s) 1735
MlyI GAGTC 2 cut(s) 513, 1894
Mox20I TGGCCA 1 cut(s) 1735
MscI TGGCCA 1 cut(s) 1735
MseI TTAA 3 cut(s) 1209, 1368, 1838
MslI CAYNNNNRTG 3 cut(s) 56, 768, 1895
Msp20I TGGCCA 1 cut(s) 1735
MspA1I CMGCKG 3 cut(s) 554, 822, 830
MspI CCGG 5 cut(s) 164, 558, 923, 1332, 1829
MspR9I CCNGG 7 cut(s) 906, 987, 1332, 1362, 1643, 1732, 1829
MvaI CCWGG 5 cut(s) 906, 987, 1362, 1643, 1732
MvnI CGCG 2 cut(s) 788, 1604
MwoI GCNNNNNNNGC 8 cut(s) 626, 635, 668, 785, 794, 934, 1091, 1326
NciI CCSGG 2 cut(s) 1332, 1829
NcoI CCATGG 1 cut(s) 529
NdeII GATC 7 cut(s) 366, 400, 940, 1028, 1464, 1528, 1574
NheI GCTAGC 2 cut(s) 578, 685
NlaIV GGNNCC 5 cut(s) 873, 1423, 1564, 1783, 1863
NmuCI GTSAC 5 cut(s) 677, 908, 1488, 1813, 1897
NspI RCATGY 3 cut(s) 73, 305, 685
PaeI GCATGC 2 cut(s) 73, 305
PagI TCATGA 1 cut(s) 1405
PaqCI CACCTGC 1 cut(s) 1501
PfeI GAWTC 4 cut(s) 746, 880, 1102, 2001
PkrI GCNGC 7 cut(s) 247, 556, 691, 1096, 1451, 1459, 1498
PleI GAGTC 2 cut(s) 512, 1894
PpsI GAGTC 2 cut(s) 512, 1894
Ppu21I YACGTR 1 cut(s) 1250
PpuMI RGGWCCY 1 cut(s) 1782
PsiI TTATAA 1 cut(s) 81
Psp1406I AACGTT 1 cut(s) 84
Psp5II RGGWCCY 1 cut(s) 1782
Psp6I CCWGG 5 cut(s) 904, 985, 1360, 1641, 1730
PspEI GGTNACC 1 cut(s) 1488
PspFI CCCAGC 2 cut(s) 1585, 1973
PspGI CCWGG 5 cut(s) 904, 985, 1360, 1641, 1730
PspN4I GGNNCC 5 cut(s) 873, 1423, 1564, 1783, 1863
PspPI GGNCC 7 cut(s) 842, 871, 902, 1313, 1358, 1782, 1826
PspPPI RGGWCCY 1 cut(s) 1782
PstNI CAGNNNCTG 1 cut(s) 611
PsuI RGATCY 1 cut(s) 1028
PvuII CAGCTG 3 cut(s) 554, 822, 830
RsaI GTAC 5 cut(s) 93, 172, 1155, 1252, 1423
RsaNI GTAC 5 cut(s) 92, 171, 1154, 1251, 1422
RseI CAYNNNNRTG 3 cut(s) 56, 768, 1895
SaqAI TTAA 3 cut(s) 1209, 1368, 1838
SatI GCNGC 7 cut(s) 246, 555, 690, 1095, 1450, 1458, 1497
Sau3AI GATC 7 cut(s) 366, 400, 940, 1028, 1464, 1528, 1574
Sau96I GGNCC 7 cut(s) 842, 871, 902, 1313, 1358, 1782, 1826
ScaI AGTACT 1 cut(s) 172
SchI GAGTC 2 cut(s) 513, 1894
ScrFI CCNGG 7 cut(s) 906, 987, 1332, 1362, 1643, 1732, 1829
SduI GDGCHC 2 cut(s) 729, 1610
SfaNI GCATC 4 cut(s) 416, 663, 1135, 1255
SfcI CTRYAG 2 cut(s) 1129, 1597
SinI GGWCC 4 cut(s) 902, 1313, 1358, 1782
SmiMI CAYNNNNRTG 3 cut(s) 56, 768, 1895
SmlI CTYRAG 1 cut(s) 129
SmoI CTYRAG 1 cut(s) 129
SphI GCATGC 2 cut(s) 73, 305
SsiI CCGC 8 cut(s) 102, 223, 354, 1439, 1449, 1509, 1560, 1604
SspMI CTAG 3 cut(s) 579, 686, 1106
StyD4I CCNGG 7 cut(s) 904, 985, 1330, 1360, 1641, 1730, 1827
StyI CCWWGG 3 cut(s) 481, 529, 875
TaaI ACNGT 3 cut(s) 445, 735, 1813
TaiI ACGT 2 cut(s) 87, 1252
TaqI TCGA 4 cut(s) 657, 863, 939, 1573
TaqII GACCGA 2 cut(s) 890, 1330
TatI WGTACW 2 cut(s) 170, 1153
TauI GCSGC 1 cut(s) 1452
TfiI GAWTC 4 cut(s) 746, 880, 1102, 2001
Tru1I TTAA 3 cut(s) 1209, 1368, 1838
Tru9I TTAA 3 cut(s) 1209, 1368, 1838
TscAI CASTG 5 cut(s) 450, 706, 738, 1561, 1914
TseFI GTSAC 5 cut(s) 677, 908, 1488, 1813, 1897
TseI GCWGC 6 cut(s) 245, 554, 689, 1094, 1457, 1496
Tsp45I GTSAC 5 cut(s) 677, 908, 1488, 1813, 1897
TspDTI ATGAA 5 cut(s) 46, 534, 1752, 1977, 1993
TspGWI ACGGA 1 cut(s) 1136
TspRI CASTG 5 cut(s) 450, 706, 738, 1561, 1914
VpaK11BI GGWCC 4 cut(s) 902, 1313, 1358, 1782
XapI RAATTY 7 cut(s) 110, 475, 810, 918, 948, 1335, 1414
XceI RCATGY 3 cut(s) 73, 305, 685
XspI CTAG 3 cut(s) 579, 686, 1106
ZrmI AGTACT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.