Rw7G016060

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000302
Physical Location & Seq
Reverse (-)
16425785 .. 16426902
1118 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G016060.1

Sequence Viewer

Length: 780 bp
ATGATCCTTATGAACGAAGAAACAAATGGCTATAGCACTTTAGCTGATGCTCATGTGCTTCCCGCAACCCATGTGAGTTATGCTGCAGGTGCAGGATCAAGCATGGTGAGCCCAGGAATCTTGAAACCTGACATCATTGGTCCTGGTGTTAGCATCTTAGCTGCATGGCCTGTTTCGGTGGATAATGGCACAGAATCTACAGCAACATTTAACATGGTTTCAGGTACCTCAATGTCATGCTCTCACCTGAGTGGCATTGCAGCTTTGCTCAAGAGCTCACGCCCTGATTGGTCACCAGCTGCCATTAAGTCTGCAATTGTGACAACTGCTCAAGTACTAAACCTCGGAGTCAAGCCCATTGTTGATGAAACACTTGGTCCAGCTGACATCTTTGCCACTGGTGCAGGCCATGTTAACCCTTCAAGAGCAAATGACCCCGGGCTAATCTACGACTTAGAACCAGCGGATTACATCCCCTATCTATGCGGTTTGAATTACAGTGACGATCAGATACAGATCATCACCCAACAAACAGTGAAATGCTCTCATGTAGGAGCCATACCAGAAGCGCAGCTAAATTGTCCTTCATTTTCTATTTTATTTGAGAACGTGACCAAGTCTCAAGCTTACACAAGGACTGTAACTAATGTTGGCCCAGCTAACTCAACTTACAGGTTAGAGGTTGGGGTACCAAGTAAAATCGCAATAGGCATTGATACTGGGGAACTTACATTTACAGAGGTTGGACAGAAGATTACATATATACCAGGTGGTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

259

Amino Acids

27.03

Weight (kDa)

4.76

Isoelectric Point (pI)

24.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 31 - 113 8.9e-14 Subtilase family
fn3_6 PF17766 191 - 254 1e-14 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 77
Acc36I ACCTGC 1 cut(s) 77
Acc65I GGTACC 2 cut(s) 224, 688
AccB1I GGYRCC 2 cut(s) 224, 688
AciI CCGC 3 cut(s) 63, 464, 486
AclWI GGATC 1 cut(s) 103
AfaI GTAC 3 cut(s) 226, 336, 690
AgsI TTSAA 3 cut(s) 124, 423, 493
AjnI CCWGG 3 cut(s) 112, 142, 766
AleI CACNNNNGTG 1 cut(s) 249
AluBI AGCT 9 cut(s) 44, 161, 263, 276, 299, 383, 574, 626, 659
AluI AGCT 9 cut(s) 44, 161, 263, 276, 299, 383, 574, 626, 659
Alw21I GWGCWC 1 cut(s) 278
Alw26I GTCTC 1 cut(s) 624
AlwI GGATC 1 cut(s) 103
Ama87I CYCGRG 1 cut(s) 437
AoxI GGCC 3 cut(s) 167, 406, 652
ApeKI GCWGC 5 cut(s) 83, 161, 260, 299, 571
Asp718I GGTACC 2 cut(s) 224, 688
AspLEI GCGC 1 cut(s) 571
AspS9I GGNCC 3 cut(s) 140, 377, 653
AsuC2I CCSGG 2 cut(s) 438, 439
AsuHPI GGTGA 4 cut(s) 118, 236, 285, 514
AvaI CYCGRG 1 cut(s) 437
AvaII GGWCC 2 cut(s) 140, 377
BanI GGYRCC 2 cut(s) 224, 688
BanII GRGCYC 2 cut(s) 113, 278
Bbv12I GWGCWC 1 cut(s) 278
BbvI GCAGC 5 cut(s) 70, 148, 272, 286, 583
BciT130I CCWGG 3 cut(s) 114, 144, 768
BcnI CCSGG 2 cut(s) 438, 439
BcoDI GTCTC 1 cut(s) 624
BfmI CTRYAG 3 cut(s) 31, 84, 198
BfuAI ACCTGC 1 cut(s) 77
BisI GCNGC 5 cut(s) 84, 162, 261, 300, 572
BlsI GCNGC 5 cut(s) 85, 163, 262, 301, 573
BmcAI AGTACT 1 cut(s) 336
Bme1390I CCNGG 5 cut(s) 114, 144, 438, 439, 768
Bme18I GGWCC 2 cut(s) 140, 377
BmeT110I CYCGRG 1 cut(s) 437
BmgT120I GGNCC 3 cut(s) 140, 377, 653
BmiI GGNNCC 3 cut(s) 226, 556, 690
BmrFI CCNGG 5 cut(s) 114, 144, 438, 439, 768
BmrI ACTGGG 1 cut(s) 729
BmsI GCATC 2 cut(s) 37, 162
BmuI ACTGGG 1 cut(s) 729
BpuEI CTTGAG 3 cut(s) 254, 315, 606
BpuMI CCSGG 2 cut(s) 438, 439
BsaBI GATNNNNATC 1 cut(s) 515
BsaJI CCNNGG 4 cut(s) 112, 343, 436, 437
Bse1I ACTGG 2 cut(s) 403, 724
Bse3DI GCAATG 1 cut(s) 255
Bse8I GATNNNNATC 1 cut(s) 515
BseBI CCWGG 3 cut(s) 114, 144, 768
BseDI CCNNGG 4 cut(s) 112, 343, 436, 437
BseGI GGATG 1 cut(s) 471
BseJI GATNNNNATC 1 cut(s) 515
BseMI GCAATG 1 cut(s) 255
BseMII CTCAG 1 cut(s) 239
BseNI ACTGG 2 cut(s) 403, 724
BseXI GCAGC 5 cut(s) 70, 148, 272, 286, 583
BseYI CCCAGC 1 cut(s) 655
BsgI GTGCAG 2 cut(s) 111, 423
BshFI GGCC 3 cut(s) 169, 408, 654
BshNI GGYRCC 2 cut(s) 224, 688
BsiHKAI GWGCWC 1 cut(s) 278
BsiHKCI CYCGRG 1 cut(s) 437
BsiSI CCGG 1 cut(s) 438
BsmAI GTCTC 1 cut(s) 624
BsnI GGCC 3 cut(s) 169, 408, 654
BsoBI CYCGRG 1 cut(s) 437
Bsp1286I GDGCHC 2 cut(s) 113, 278
Bsp143I GATC 4 cut(s) 3, 95, 505, 516
BspACI CCGC 3 cut(s) 63, 464, 486
BspANI GGCC 3 cut(s) 169, 408, 654
BspCNI CTCAG 1 cut(s) 240
BspLI GGNNCC 3 cut(s) 226, 556, 690
BspMAI CTGCAG 1 cut(s) 88
BspMI ACCTGC 1 cut(s) 77
BspPI GGATC 1 cut(s) 103
BspT107I GGYRCC 2 cut(s) 224, 688
BsrDI GCAATG 1 cut(s) 255
BsrI ACTGG 2 cut(s) 403, 724
BssECI CCNNGG 4 cut(s) 112, 343, 436, 437
BssMI GATC 4 cut(s) 3, 95, 505, 516
Bst2UI CCWGG 3 cut(s) 114, 144, 768
Bst4CI ACNGT 3 cut(s) 500, 535, 640
BstC8I GCNNGC 1 cut(s) 406
BstDEI CTNAG 3 cut(s) 157, 248, 454
BstEII GGTNACC 1 cut(s) 291
BstF5I GGATG 1 cut(s) 471
BstHHI GCGC 1 cut(s) 571
BstKTI GATC 4 cut(s) 6, 98, 508, 519
BstMAI GTCTC 1 cut(s) 624
BstMBI GATC 4 cut(s) 3, 95, 505, 516
BstMWI GCNNNNNNNGC 3 cut(s) 89, 108, 401
BstNI CCWGG 3 cut(s) 114, 144, 768
BstPI GGTNACC 1 cut(s) 291
BstSCI CCNGG 5 cut(s) 112, 142, 436, 437, 766
BstSFI CTRYAG 3 cut(s) 31, 84, 198
BstV1I GCAGC 5 cut(s) 70, 148, 272, 286, 583
BsuRI GGCC 3 cut(s) 169, 408, 654
BtsCI GGATG 1 cut(s) 471
BtsIMutI CAGTG 3 cut(s) 396, 505, 540
BveI ACCTGC 1 cut(s) 77
Cac8I GCNNGC 1 cut(s) 406
CfoI GCGC 1 cut(s) 571
Cfr13I GGNCC 3 cut(s) 140, 377, 653
Cfr9I CCCGGG 1 cut(s) 437
CsiI ACCWGGT 1 cut(s) 766
Csp6I GTAC 3 cut(s) 225, 335, 689
CviAII CATG 8 cut(s) 53, 71, 103, 165, 214, 237, 410, 548
CviQI GTAC 3 cut(s) 225, 335, 689
DdeI CTNAG 3 cut(s) 157, 248, 454
DpnI GATC 4 cut(s) 5, 97, 507, 518
DpnII GATC 4 cut(s) 3, 95, 505, 516
Ecl136II GAGCTC 1 cut(s) 276
Eco24I GRGCYC 2 cut(s) 113, 278
Eco47I GGWCC 2 cut(s) 140, 377
Eco53kI GAGCTC 1 cut(s) 276
Eco88I CYCGRG 1 cut(s) 437
Eco91I GGTNACC 1 cut(s) 291
EcoICRI GAGCTC 1 cut(s) 276
EcoO65I GGTNACC 1 cut(s) 291
EcoRII CCWGG 3 cut(s) 112, 142, 766
EcoT38I GRGCYC 2 cut(s) 113, 278
FaeI CATG 8 cut(s) 56, 74, 106, 168, 217, 240, 413, 551
FatI CATG 8 cut(s) 52, 70, 102, 164, 213, 236, 409, 547
FauI CCCGC 1 cut(s) 70
Fnu4HI GCNGC 5 cut(s) 84, 162, 261, 300, 572
FokI GGATG 1 cut(s) 458
FriOI GRGCYC 2 cut(s) 113, 278
Fsp4HI GCNGC 5 cut(s) 84, 162, 261, 300, 572
GlaI GCGC 1 cut(s) 570
GluI GCNGC 5 cut(s) 84, 162, 261, 300, 572
GsaI CCCAGC 1 cut(s) 659
HaeIII GGCC 3 cut(s) 169, 408, 654
HapII CCGG 1 cut(s) 438
HhaI GCGC 1 cut(s) 571
Hin1II CATG 8 cut(s) 56, 74, 106, 168, 217, 240, 413, 551
Hin6I GCGC 1 cut(s) 569
HinP1I GCGC 1 cut(s) 569
HincII GTYRAC 1 cut(s) 415
HindII GTYRAC 1 cut(s) 415
HindIII AAGCTT 1 cut(s) 624
HinfI GANTC 3 cut(s) 117, 194, 348
HpaI GTTAAC 1 cut(s) 415
HpaII CCGG 1 cut(s) 438
HphI GGTGA 4 cut(s) 118, 236, 285, 514
Hpy166II GTNNAC 1 cut(s) 415
Hpy188I TCNGA 2 cut(s) 347, 510
Hpy188III TCNNGA 3 cut(s) 121, 271, 423
Hpy8I GTNNAC 1 cut(s) 415
HpyAV CCTTC 2 cut(s) 429, 594
HpyCH4III ACNGT 3 cut(s) 500, 535, 640
HpyCH4IV ACGT 1 cut(s) 609
HpyCH4V TGCA 6 cut(s) 86, 92, 164, 260, 314, 404
HpyF10VI GCNNNNNNNGC 3 cut(s) 89, 108, 401
HpyF3I CTNAG 3 cut(s) 157, 248, 454
HpySE526I ACGT 1 cut(s) 609
Hsp92II CATG 8 cut(s) 56, 74, 106, 168, 217, 240, 413, 551
HspAI GCGC 1 cut(s) 569
KpnI GGTACC 2 cut(s) 228, 692
KspAI GTTAAC 1 cut(s) 415
Kzo9I GATC 4 cut(s) 3, 95, 505, 516
LmnI GCTCC 1 cut(s) 554
Lsp1109I GCAGC 5 cut(s) 70, 148, 272, 286, 583
LweI GCATC 2 cut(s) 37, 162
MabI ACCWGGT 1 cut(s) 766
MaeII ACGT 1 cut(s) 609
MaeIII GTNAC 5 cut(s) 291, 319, 500, 610, 640
MalI GATC 4 cut(s) 5, 97, 507, 518
MboI GATC 4 cut(s) 3, 95, 505, 516
MboII GAAGA 2 cut(s) 29, 763
MfeI CAATTG 1 cut(s) 315
MhlI GDGCHC 2 cut(s) 113, 278
MluCI AATT 3 cut(s) 315, 493, 577
MlyI GAGTC 1 cut(s) 357
MmeI TCCRAC 1 cut(s) 724
MnlI CCTC 4 cut(s) 238, 353, 673, 733
MseI TTAA 4 cut(s) 210, 306, 414, 778
MslI CAYNNNNRTG 1 cut(s) 249
MspA1I CMGCKG 3 cut(s) 299, 383, 464
MspI CCGG 1 cut(s) 438
MspR9I CCNGG 5 cut(s) 114, 144, 438, 439, 768
MunI CAATTG 1 cut(s) 315
MvaI CCWGG 3 cut(s) 114, 144, 768
MwoI GCNNNNNNNGC 3 cut(s) 89, 108, 401
NciI CCSGG 2 cut(s) 438, 439
NdeII GATC 4 cut(s) 3, 95, 505, 516
NlaIII CATG 8 cut(s) 56, 74, 106, 168, 217, 240, 413, 551
NlaIV GGNNCC 3 cut(s) 226, 556, 690
NmuCI GTSAC 4 cut(s) 291, 319, 500, 610
OliI CACNNNNGTG 1 cut(s) 249
PaqCI CACCTGC 1 cut(s) 77
PfeI GAWTC 2 cut(s) 117, 194
PflFI GACNNNGTC 1 cut(s) 616
PkrI GCNGC 5 cut(s) 85, 163, 262, 301, 573
PleI GAGTC 1 cut(s) 356
PpsI GAGTC 1 cut(s) 356
Psp124BI GAGCTC 1 cut(s) 278
Psp6I CCWGG 3 cut(s) 112, 142, 766
PspEI GGTNACC 1 cut(s) 291
PspFI CCCAGC 1 cut(s) 655
PspGI CCWGG 3 cut(s) 112, 142, 766
PspN4I GGNNCC 3 cut(s) 226, 556, 690
PspPI GGNCC 3 cut(s) 140, 377, 653
PstI CTGCAG 1 cut(s) 88
PsyI GACNNNGTC 1 cut(s) 616
PvuII CAGCTG 2 cut(s) 299, 383
RsaI GTAC 3 cut(s) 226, 336, 690
RsaNI GTAC 3 cut(s) 225, 335, 689
RseI CAYNNNNRTG 1 cut(s) 249
SacI GAGCTC 1 cut(s) 278
SaqAI TTAA 4 cut(s) 210, 306, 414, 778
SatI GCNGC 5 cut(s) 84, 162, 261, 300, 572
Sau3AI GATC 4 cut(s) 3, 95, 505, 516
Sau96I GGNCC 3 cut(s) 140, 377, 653
ScaI AGTACT 1 cut(s) 336
SchI GAGTC 1 cut(s) 357
ScrFI CCNGG 5 cut(s) 114, 144, 438, 439, 768
SduI GDGCHC 2 cut(s) 113, 278
SexAI ACCWGGT 1 cut(s) 766
SfaNI GCATC 2 cut(s) 37, 162
SfcI CTRYAG 3 cut(s) 31, 84, 198
SinI GGWCC 2 cut(s) 140, 377
SmaI CCCGGG 1 cut(s) 439
SmiMI CAYNNNNRTG 1 cut(s) 249
SmlI CTYRAG 3 cut(s) 269, 330, 621
SmoI CTYRAG 3 cut(s) 269, 330, 621
Sse9I AATT 3 cut(s) 315, 493, 577
SsiI CCGC 3 cut(s) 63, 464, 486
SstI GAGCTC 1 cut(s) 278
StyD4I CCNGG 5 cut(s) 112, 142, 436, 437, 766
TaaI ACNGT 3 cut(s) 500, 535, 640
TaiI ACGT 1 cut(s) 612
TasI AATT 3 cut(s) 315, 493, 577
TatI WGTACW 1 cut(s) 334
TfiI GAWTC 2 cut(s) 117, 194
Tru1I TTAA 4 cut(s) 210, 306, 414, 778
Tru9I TTAA 4 cut(s) 210, 306, 414, 778
TscAI CASTG 3 cut(s) 403, 505, 540
TseFI GTSAC 4 cut(s) 291, 319, 500, 610
TseI GCWGC 5 cut(s) 83, 161, 260, 299, 571
Tsp45I GTSAC 4 cut(s) 291, 319, 500, 610
TspDTI ATGAA 3 cut(s) 26, 381, 576
TspMI CCCGGG 1 cut(s) 437
TspRI CASTG 3 cut(s) 403, 505, 540
Tth111I GACNNNGTC 1 cut(s) 616
VpaK11BI GGWCC 2 cut(s) 140, 377
XmaI CCCGGG 1 cut(s) 437
ZrmI AGTACT 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.