MD04G1072000.v1.1

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000302
Physical Location & Seq
Reverse (-)
9779142 .. 9780445
1304 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1072000.v1.1.491

Sequence Viewer

Length: 1254 bp
ATGGAGAACAAAAAAGGTGCACCAACGCATGTCATATATCTTCTTGGTTTTACTTTGATCATGTTTTACTTATCAAAGGCAGTTGCACAAGAGGAAAAAAATAGCCTCCAAACTTACATTGTTTTTCTCGAAAAGCCGGCCTCCTCGAAATTTTCTGAAGAATCAAATTTAGATAGTTGGTATCAGTCATTTTTGCCTGAAACTACATCAAACTTAAACCAGCAGAGGATCGTTCATGCCTACCGCAGTGTGGTCACTGGATTTGCAGCTAAACTGACACCAGAGGAAGTGAAAGCAATGGAAAACAAAGAAGGGTTTTTGTCGGCTCATATCGAGAAAACGGTTCCTTTGCTAACTACTCATAGTCCTGACTTCTTGGGGTTACACCAAGGATACGGACTATGGAAACAAGCAAATTACGGTGAAGGTTTGATAATAGGACTCTTGGATACAGGCATTGGACCTGATCATCCTTCATTCAGTGATGAAGGAGTATCCCCGCCTCCAGCTACATGGAAGGGCAAGTGCGACTTCAATGGAACAGTGTGCAACAACAAGCTCATTGGTGCACAAAATTTCGTTGGTGCAGAAGAAGGGAATATTACAGGAAATCCATTTGATCTCGTTGGCCATGGCACCCACACTTCTAGCACCGCTGCTGGAAATTTTGTGGAAGGTGCTAGCGTGTTTGGAGAGGCCAATGGCACGGCTGTTGGCATGGCACCTTATGCTCACTTGGCAATGTACAAAGTCTGTTATGGGATTGGTTGTAGGTACGGCGACATCTTAGCTGGTCTTGATGCCGCTGTTGAAGATGGCTTGGACGTGCTTTCCCTTTCACTTGGAGGCCCCTCACTTCCTTTCTACAATGATGTCATTGCAATCGGTGCATTTGCAGCAATTAAAAAAGGAATATTTTTCAGTTGTGCAGCTGGAAATTCTGGTCCCTATCATGAGTCTTTATCAAATGAGGCGCCATGGATTTTCACAGTCGGAGCAAGTACCACTGACAGAATACTAAAATCACAAGATGAGAAGCTCAACACCTATGGCAGTATAATCGGAGATGCACTTGCTCCCAAGGTAGCTTCCTTTTCGTCAAGAGGACCAAGCCTTTCAAGCCCCGGAATTCTGAAACCTGACATCATTGGACCCGGCGTTGACATCCTAGCAGCATGGCCTGAATCAGTAGACAATGCCACACTTCCTAATCCTAAGGCAACATTTAACATAATTTCTGAAATCACACCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

418

Amino Acids

44.29

Weight (kDa)

5.08

Isoelectric Point (pI)

34.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 38 - 115 3.5e-10 Peptidase inhibitor I9
Peptidase_S8 PF00082 141 - 388 1.8e-26 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 635, 721, 973
AccI GTMKAC 1 cut(s) 1191
AciI CCGC 4 cut(s) 244, 500, 654, 804
AclWI GGATC 1 cut(s) 236
AcoI YGGCCR 1 cut(s) 628
AcsI RAATTY 6 cut(s) 149, 166, 574, 664, 937, 1128
AcuI CTGAAG 1 cut(s) 177
AcyI GRCGYC 1 cut(s) 974
AfaI GTAC 3 cut(s) 746, 776, 1003
AfiI CCNNNNNNNGG 1 cut(s) 250
AgsI TTSAA 3 cut(s) 535, 812, 1119
AjiI CACGTC 1 cut(s) 826
AluBI AGCT 7 cut(s) 269, 509, 559, 791, 932, 1039, 1088
AluI AGCT 7 cut(s) 269, 509, 559, 791, 932, 1039, 1088
Alw21I GWGCWC 2 cut(s) 22, 571
Alw44I GTGCAC 2 cut(s) 18, 567
AlwI GGATC 1 cut(s) 236
AoxI GGCC 5 cut(s) 138, 628, 696, 847, 1178
ApaLI GTGCAC 2 cut(s) 18, 567
ApeKI GCWGC 5 cut(s) 266, 656, 896, 929, 1172
ApoI RAATTY 6 cut(s) 149, 166, 574, 664, 937, 1128
AspLEI GCGC 1 cut(s) 976
AspS9I GGNCC 5 cut(s) 461, 848, 944, 1106, 1151
AsuC2I CCSGG 2 cut(s) 1125, 1155
AsuHPI GGTGA 1 cut(s) 434
AsuNHI GCTAGC 1 cut(s) 680
AvaII GGWCC 4 cut(s) 461, 944, 1106, 1151
AxyI CCTNAGG 1 cut(s) 1215
BaeGI GKGCMC 2 cut(s) 22, 571
BalI TGGCCA 1 cut(s) 630
BanI GGYRCC 3 cut(s) 635, 721, 973
Bbv12I GWGCWC 2 cut(s) 22, 571
BbvI GCAGC 5 cut(s) 278, 643, 908, 941, 1184
BccI CCATC 1 cut(s) 809
BceAI ACGGC 2 cut(s) 723, 793
BcgI CGANNNNNNTGC 2 cut(s) 1042, 1076
BciVI GTATCC 3 cut(s) 386, 442, 505
BclI TGATCA 2 cut(s) 57, 466
BcnI CCSGG 2 cut(s) 1125, 1155
BfaI CTAG 3 cut(s) 648, 681, 1169
BfoI RGCGCY 1 cut(s) 977
BfuI GTATCC 3 cut(s) 386, 442, 505
BisI GCNGC 6 cut(s) 267, 657, 804, 897, 930, 1173
BlsI GCNGC 6 cut(s) 268, 658, 805, 898, 931, 1174
Bme1390I CCNGG 2 cut(s) 1125, 1155
Bme18I GGWCC 4 cut(s) 461, 944, 1106, 1151
BmgBI CACGTC 1 cut(s) 826
BmgT120I GGNCC 5 cut(s) 461, 848, 944, 1106, 1151
BmiI GGNNCC 7 cut(s) 345, 637, 723, 850, 946, 975, 1153
BmrFI CCNGG 2 cut(s) 1125, 1155
BmsI GCATC 2 cut(s) 790, 1057
BmtI GCTAGC 1 cut(s) 684
BpmI CTGGAG 1 cut(s) 489
BpuMI CCSGG 2 cut(s) 1125, 1155
BsaHI GRCGYC 1 cut(s) 974
BsaJI CCNNGG 5 cut(s) 388, 631, 977, 1080, 1123
Bsc4I CCNNNNNNNGG 1 cut(s) 250
Bse118I RCCGGY 1 cut(s) 136
Bse1I ACTGG 1 cut(s) 262
Bse21I CCTNAGG 1 cut(s) 1215
Bse3DI GCAATG 3 cut(s) 303, 747, 876
BseDI CCNNGG 5 cut(s) 388, 631, 977, 1080, 1123
BseGI GGATG 2 cut(s) 469, 1164
BseLI CCNNNNNNNGG 1 cut(s) 250
BseMI GCAATG 3 cut(s) 303, 747, 876
BseNI ACTGG 1 cut(s) 262
BseRI GAGGAG 1 cut(s) 133
BseSI GKGCMC 2 cut(s) 22, 571
BseXI GCAGC 5 cut(s) 278, 643, 908, 941, 1184
BsgI GTGCAG 2 cut(s) 606, 948
BshFI GGCC 5 cut(s) 140, 630, 698, 849, 1180
BshNI GGYRCC 3 cut(s) 635, 721, 973
BsiHKAI GWGCWC 2 cut(s) 22, 571
BsiSI CCGG 3 cut(s) 137, 1125, 1155
BslFI GGGAC 1 cut(s) 930
BslI CCNNNNNNNGG 1 cut(s) 250
BsmFI GGGAC 1 cut(s) 930
BsnI GGCC 5 cut(s) 140, 630, 698, 849, 1180
Bsp1286I GDGCHC 2 cut(s) 22, 571
Bsp1407I TGTACA 1 cut(s) 744
Bsp143I GATC 4 cut(s) 57, 228, 466, 619
Bsp19I CCATGG 2 cut(s) 631, 977
BspACI CCGC 4 cut(s) 244, 500, 654, 804
BspANI GGCC 5 cut(s) 140, 630, 698, 849, 1180
BspHI TCATGA 1 cut(s) 952
BspLI GGNNCC 7 cut(s) 345, 637, 723, 850, 946, 975, 1153
BspOI GCTAGC 1 cut(s) 684
BspPI GGATC 1 cut(s) 236
BspT107I GGYRCC 3 cut(s) 635, 721, 973
BsrDI GCAATG 3 cut(s) 303, 747, 876
BsrFI RCCGGY 1 cut(s) 136
BsrGI TGTACA 1 cut(s) 744
BsrI ACTGG 1 cut(s) 262
BssAI RCCGGY 1 cut(s) 136
BssECI CCNNGG 5 cut(s) 388, 631, 977, 1080, 1123
BssMI GATC 4 cut(s) 57, 228, 466, 619
BssNI GRCGYC 1 cut(s) 974
BssT1I CCWWGG 4 cut(s) 388, 631, 977, 1080
Bst4CI ACNGT 4 cut(s) 343, 422, 544, 991
BstACI GRCGYC 1 cut(s) 974
BstAPI GCANNNNNTGC 2 cut(s) 728, 887
BstAUI TGTACA 1 cut(s) 744
BstC8I GCNNGC 2 cut(s) 138, 682
BstDEI CTNAG 2 cut(s) 787, 1215
BstDSI CCRYGG 2 cut(s) 631, 977
BstF5I GGATG 2 cut(s) 469, 1164
BstH2I RGCGCY 1 cut(s) 977
BstHHI GCGC 1 cut(s) 976
BstKTI GATC 4 cut(s) 60, 231, 469, 622
BstMBI GATC 4 cut(s) 57, 228, 466, 619
BstMWI GCNNNNNNNGC 5 cut(s) 728, 737, 887, 896, 1119
BstNSI RCATGY 1 cut(s) 32
BstSCI CCNGG 2 cut(s) 1123, 1153
BstSLI GKGCMC 2 cut(s) 22, 571
BstV1I GCAGC 5 cut(s) 278, 643, 908, 941, 1184
BstXI CCANNNNNNTGG 1 cut(s) 513
Bsu36I CCTNAGG 1 cut(s) 1215
BsuI GTATCC 3 cut(s) 386, 442, 505
BsuRI GGCC 5 cut(s) 140, 630, 698, 849, 1180
BtgI CCRYGG 2 cut(s) 631, 977
BtrI CACGTC 1 cut(s) 826
BtsCI GGATG 2 cut(s) 469, 1164
BtsI GCAGTG 1 cut(s) 253
BtsIMutI CAGTG 5 cut(s) 253, 255, 487, 549, 1005
Cac8I GCNNGC 2 cut(s) 138, 682
CciI TCATGA 1 cut(s) 952
CfoI GCGC 1 cut(s) 976
Cfr10I RCCGGY 1 cut(s) 136
Cfr13I GGNCC 5 cut(s) 461, 848, 944, 1106, 1151
Csp6I GTAC 3 cut(s) 745, 775, 1002
CviAII CATG 9 cut(s) 29, 61, 236, 513, 632, 718, 953, 978, 1176
CviQI GTAC 3 cut(s) 745, 775, 1002
DdeI CTNAG 2 cut(s) 787, 1215
DinI GGCGCC 1 cut(s) 975
DpnI GATC 4 cut(s) 59, 230, 468, 621
DpnII GATC 4 cut(s) 57, 228, 466, 619
EaeI YGGCCR 1 cut(s) 628
Eco130I CCWWGG 4 cut(s) 388, 631, 977, 1080
Eco47I GGWCC 4 cut(s) 461, 944, 1106, 1151
Eco57I CTGAAG 1 cut(s) 177
Eco81I CCTNAGG 1 cut(s) 1215
EcoO109I RGGNCCY 1 cut(s) 848
EcoRI GAATTC 1 cut(s) 1128
EcoT14I CCWWGG 4 cut(s) 388, 631, 977, 1080
EgeI GGCGCC 1 cut(s) 975
EheI GGCGCC 1 cut(s) 975
ErhI CCWWGG 4 cut(s) 388, 631, 977, 1080
FaeI CATG 9 cut(s) 32, 64, 239, 516, 635, 721, 956, 981, 1179
FalI AAGNNNNNCTT 2 cut(s) 515, 547
FaqI GGGAC 1 cut(s) 930
FatI CATG 9 cut(s) 28, 60, 235, 512, 631, 717, 952, 977, 1175
FauI CCCGC 1 cut(s) 507
FbaI TGATCA 2 cut(s) 57, 466
FblI GTMKAC 1 cut(s) 1191
Fnu4HI GCNGC 6 cut(s) 267, 657, 804, 897, 930, 1173
FokI GGATG 2 cut(s) 456, 1151
Fsp4HI GCNGC 6 cut(s) 267, 657, 804, 897, 930, 1173
FspBI CTAG 3 cut(s) 648, 681, 1169
GlaI GCGC 1 cut(s) 975
GluI GCNGC 6 cut(s) 267, 657, 804, 897, 930, 1173
GsuI CTGGAG 1 cut(s) 489
HaeII RGCGCY 1 cut(s) 977
HaeIII GGCC 5 cut(s) 140, 630, 698, 849, 1180
HapII CCGG 3 cut(s) 137, 1125, 1155
HhaI GCGC 1 cut(s) 976
Hin1I GRCGYC 1 cut(s) 974
Hin1II CATG 9 cut(s) 32, 64, 239, 516, 635, 721, 956, 981, 1179
Hin6I GCGC 1 cut(s) 974
HinP1I GCGC 1 cut(s) 974
HincII GTYRAC 1 cut(s) 1162
HindII GTYRAC 1 cut(s) 1162
HinfI GANTC 4 cut(s) 161, 441, 956, 1184
HpaII CCGG 3 cut(s) 137, 1125, 1155
HphI GGTGA 1 cut(s) 434
Hpy166II GTNNAC 4 cut(s) 20, 569, 1162, 1192
Hpy188I TCNGA 5 cut(s) 157, 995, 1064, 1134, 1240
Hpy188III TCNNGA 6 cut(s) 128, 334, 368, 797, 953, 1101
Hpy8I GTNNAC 4 cut(s) 20, 569, 1162, 1192
HpyAV CCTTC 7 cut(s) 305, 419, 482, 483, 511, 587, 668
HpyCH4III ACNGT 4 cut(s) 343, 422, 544, 991
HpyCH4IV ACGT 1 cut(s) 825
HpyF10VI GCNNNNNNNGC 5 cut(s) 728, 737, 887, 896, 1119
HpyF3I CTNAG 2 cut(s) 787, 1215
HpySE526I ACGT 1 cut(s) 825
Hsp92I GRCGYC 1 cut(s) 974
Hsp92II CATG 9 cut(s) 32, 64, 239, 516, 635, 721, 956, 981, 1179
HspAI GCGC 1 cut(s) 974
KasI GGCGCC 1 cut(s) 973
KroI GCCGGC 1 cut(s) 136
KroNI GCCGGC 1 cut(s) 138
Ksp22I TGATCA 2 cut(s) 57, 466
Kzo9I GATC 4 cut(s) 57, 228, 466, 619
LmnI GCTCC 2 cut(s) 995, 1081
Lsp1109I GCAGC 5 cut(s) 278, 643, 908, 941, 1184
LweI GCATC 2 cut(s) 790, 1057
MaeI CTAG 3 cut(s) 648, 681, 1169
MaeII ACGT 1 cut(s) 825
MaeIII GTNAC 2 cut(s) 253, 381
MalI GATC 4 cut(s) 59, 230, 468, 621
MboI GATC 4 cut(s) 57, 228, 466, 619
MboII GAAGA 4 cut(s) 32, 170, 602, 824
MhlI GDGCHC 2 cut(s) 22, 571
MlsI TGGCCA 1 cut(s) 630
MluCI AATT 9 cut(s) 149, 166, 415, 574, 664, 900, 937, 1128, 1233
MluNI TGGCCA 1 cut(s) 630
Mly113I GGCGCC 1 cut(s) 974
MlyI GAGTC 2 cut(s) 435, 965
MmeI TCCRAC 1 cut(s) 973
Mox20I TGGCCA 1 cut(s) 630
MroNI GCCGGC 1 cut(s) 136
MscI TGGCCA 1 cut(s) 630
MseI TTAA 3 cut(s) 215, 903, 1227
Msp20I TGGCCA 1 cut(s) 630
MspA1I CMGCKG 3 cut(s) 656, 806, 932
MspI CCGG 3 cut(s) 137, 1125, 1155
MspR9I CCNGG 2 cut(s) 1125, 1155
MwoI GCNNNNNNNGC 5 cut(s) 728, 737, 887, 896, 1119
NaeI GCCGGC 1 cut(s) 138
NarI GGCGCC 1 cut(s) 974
NciI CCSGG 2 cut(s) 1125, 1155
NcoI CCATGG 2 cut(s) 631, 977
NdeII GATC 4 cut(s) 57, 228, 466, 619
NgoMIV GCCGGC 1 cut(s) 136
NheI GCTAGC 1 cut(s) 680
NlaIII CATG 9 cut(s) 32, 64, 239, 516, 635, 721, 956, 981, 1179
NlaIV GGNNCC 7 cut(s) 345, 637, 723, 850, 946, 975, 1153
NmuCI GTSAC 1 cut(s) 253
NspI RCATGY 1 cut(s) 32
PagI TCATGA 1 cut(s) 952
PdiI GCCGGC 1 cut(s) 138
PfeI GAWTC 2 cut(s) 161, 1184
PkrI GCNGC 6 cut(s) 268, 658, 805, 898, 931, 1174
PleI GAGTC 2 cut(s) 435, 964
PluTI GGCGCC 1 cut(s) 977
PpsI GAGTC 2 cut(s) 435, 964
PspN4I GGNNCC 7 cut(s) 345, 637, 723, 850, 946, 975, 1153
PspPI GGNCC 5 cut(s) 461, 848, 944, 1106, 1151
PvuII CAGCTG 1 cut(s) 932
RsaI GTAC 3 cut(s) 746, 776, 1003
RsaNI GTAC 3 cut(s) 745, 775, 1002
SaqAI TTAA 3 cut(s) 215, 903, 1227
SatI GCNGC 6 cut(s) 267, 657, 804, 897, 930, 1173
Sau3AI GATC 4 cut(s) 57, 228, 466, 619
Sau96I GGNCC 5 cut(s) 461, 848, 944, 1106, 1151
SchI GAGTC 2 cut(s) 435, 965
ScrFI CCNGG 2 cut(s) 1125, 1155
SduI GDGCHC 2 cut(s) 22, 571
SfaNI GCATC 2 cut(s) 790, 1057
SfoI GGCGCC 1 cut(s) 975
SinI GGWCC 4 cut(s) 461, 944, 1106, 1151
Sse9I AATT 9 cut(s) 149, 166, 415, 574, 664, 900, 937, 1128, 1233
SsiI CCGC 4 cut(s) 244, 500, 654, 804
SspDI GGCGCC 1 cut(s) 973
SspI AATATT 2 cut(s) 601, 915
SspMI CTAG 3 cut(s) 648, 681, 1169
StyD4I CCNGG 2 cut(s) 1123, 1153
StyI CCWWGG 4 cut(s) 388, 631, 977, 1080
TaaI ACNGT 4 cut(s) 343, 422, 544, 991
TaiI ACGT 1 cut(s) 828
TaqI TCGA 3 cut(s) 129, 146, 333
TasI AATT 9 cut(s) 149, 166, 415, 574, 664, 900, 937, 1128, 1233
TatI WGTACW 1 cut(s) 744
TauI GCSGC 1 cut(s) 806
TfiI GAWTC 2 cut(s) 161, 1184
Tru1I TTAA 3 cut(s) 215, 903, 1227
Tru9I TTAA 3 cut(s) 215, 903, 1227
TscAI CASTG 5 cut(s) 253, 262, 487, 549, 1012
TseFI GTSAC 1 cut(s) 253
TseI GCWGC 5 cut(s) 266, 656, 896, 929, 1172
Tsp45I GTSAC 1 cut(s) 253
TspDTI ATGAA 3 cut(s) 224, 465, 501
TspGWI ACGGA 1 cut(s) 411
TspRI CASTG 5 cut(s) 253, 262, 487, 549, 1012
VneI GTGCAC 2 cut(s) 18, 567
VpaK11BI GGWCC 4 cut(s) 461, 944, 1106, 1151
XapI RAATTY 6 cut(s) 149, 166, 574, 664, 937, 1128
XceI RCATGY 1 cut(s) 32
XmiI GTMKAC 1 cut(s) 1191
XspI CTAG 3 cut(s) 648, 681, 1169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.