Rh7DG187400

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000302
Physical Location & Seq
Reverse (-)
17359124 .. 17361382
2259 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG187400.1

Sequence Viewer

Length: 1542 bp
ATGGCTGTGATTCCATTTCTTTTTCTTGTTTTCATGCTTAGCTTTTCTCATGGGATTGCTCAGGAAACCGAGCTTTCTCCTGTCCCTACGAAAACGAGCAATTTACAGACATACATTGTCCATGTAATGCAACCAGAGGGTAAAGTTTTTGCCCAAAGAGAAGACCTGGCGAGCTGGCACAAATCTTTTCTGCCATCTATGACGGCAAGCTCTGATGAGCAAACACGCTTGCTTTACTCCTACAAAACTGTGATCAGTGGTTTTTCAGCAAGGCTAACTCAAGAGGAAGTGAAAGCGATGGAACAGATGAATGGTTTTGTGGCGGCACATCCTGAACGAGTGTTTCGTCGGAAAACCACACACACTCCCAACTTCTTGGGGCTTAACCAACAAGCGGGAATTTGGAAAGACTCAAACTTTGGGAAGGGAGTGATCATTGGAGTACTGGATGGCGGAGTATTCCCCAGCCACCCTTCATTCAGTGGTGCAGGAATTCCACCTCCACCTGCTAAATGGAAAGGGAGGTGTGACTTCAATGTGTCAGAATGCAATAATAAATTAATTGGAGCACAGTCGTTCAATCTTGCAGCCATGGCATTGAAGGGAGCAAAAGCTGAACCGCCAACTGATGAAGACGGACATGGAACACACACAGCAAGCACAGCTGGTGGAGCGTTTGTGCAGAATGCTGATGCGCTAGGAAATGCCAAAGGCACAGCAGTTGGGATGGCACCTTATGCTCACCTGGCAATATACAAAGTCTGTTTTGGAGAACCCTGTCCAGAAAGTGATATCCTAGCTGCCCTTGAAGCTGCTGTTCACGATGTTGGAGCAAGCACCCTCGATAGACACATTGTAGCCTCAGCCAAGCTTGGAAATGGATTAGTATTTGACGGTGAATCTCTCTTTCAACCGAAAGATTTCCCTTCAACATTGTTGCCTCTTGTTTATGCTGGAGTAAATGGCAAAGCAGAGTCTGCATTTTGCGCCGAAGGATCCTTAAAAAACATAAGTTTGAAAGGCAAACGGATTTTTGATGAAACACTTCAGACTGCAGATATCTTAGCCACCGGTGCGGGCCATGTCAATCCATCAAAGGCGACCGACCCAGGACTGATATATGATATTCAACCTGATGATTATATTCCATATCTTTGTGGCTTGGGCTACAACGATACTGGAGTCAGTATCCTCGCGCACAGACCAATAAAGTGCTCAAAGGTATCAAGCATCCCTGAAGGAGAGCTGAACTACCCTTCATTTTCTGTCAAGCTGGGACCATCTCAGACATTCACAAGAACTGTGACAAATGTTGGTGCCTCATATTCCACTTATTCGGTCAAGGTATATGCACCACTAGGAACCCATGTGACTGTCAAACCCAGCACGCTTTACTTTACAGAGGTGAACCAGAAGGCGTCATATTCAGTGACATTCAGTCGTACTTCAGGAGGTAAAGCTGGTGCATTCACACAGGGATTTATAACATGGGCTTCTACTAATCACGTTGTCCGGAGTCCAGTTTCTGTTTTATTTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

513

Amino Acids

54.86

Weight (kDa)

7.24

Isoelectric Point (pI)

31.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 37 - 115 1.3e-13 Peptidase inhibitor I9
Peptidase_S8 PF00082 141 - 273 2e-11 Subtilase family
fn3_6 PF17766 415 - 510 7.2e-29 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1484
AarI CACCTGC 1 cut(s) 514
Acc36I ACCTGC 1 cut(s) 514
AccB1I GGYRCC 2 cut(s) 730, 1316
AccII CGCG 1 cut(s) 1196
AccIII TCCGGA 1 cut(s) 1512
AciI CCGC 5 cut(s) 323, 395, 453, 620, 1076
AclWI GGATC 2 cut(s) 990, 1003
AcsI RAATTY 2 cut(s) 399, 492
AcuI CTGAAG 3 cut(s) 1031, 1257, 1431
AcyI GRCGYC 1 cut(s) 1418
AfaI GTAC 2 cut(s) 444, 1444
AfiI CCNNNNNNNGG 1 cut(s) 394
AgeI ACCGGT 1 cut(s) 1070
AgsI TTSAA 8 cut(s) 535, 580, 601, 809, 911, 930, 1018, 1130
AhdI GACNNNNNGTC 1 cut(s) 1437
AjnI CCWGG 3 cut(s) 165, 744, 1108
Alw21I GWGCWC 2 cut(s) 571, 1217
AlwI GGATC 2 cut(s) 990, 1003
AlwNI CAGNNNCTG 2 cut(s) 977, 1526
Aor13HI TCCGGA 1 cut(s) 1512
AoxI GGCC 1 cut(s) 1078
ApeKI GCWGC 3 cut(s) 587, 800, 812
ApoI RAATTY 2 cut(s) 399, 492
ArsI GACNNNNNNTTYG 2 cut(s) 401, 433
AseI ATTAAT 1 cut(s) 560
AsiGI ACCGGT 1 cut(s) 1070
Asp700I GAANNNNTTC 2 cut(s) 920, 1044
AspLEI GCGC 3 cut(s) 697, 989, 1198
AspS9I GGNCC 2 cut(s) 1078, 1277
AsuHPI GGTGA 3 cut(s) 734, 908, 1417
AvaII GGWCC 1 cut(s) 1277
BaeI ACNNNNGTAYC 2 cut(s) 1167, 1200
BamHI GGATCC 1 cut(s) 995
BanI GGYRCC 2 cut(s) 730, 1316
BbsI GAAGAC 2 cut(s) 168, 639
Bbv12I GWGCWC 2 cut(s) 571, 1217
BbvCI CCTCAGC 1 cut(s) 862
BbvI GCAGC 3 cut(s) 599, 787, 799
BccI CCATC 6 cut(s) 202, 292, 443, 721, 1099, 1288
BceAI ACGGC 1 cut(s) 219
BciT130I CCWGG 3 cut(s) 167, 746, 1110
BciVI GTATCC 1 cut(s) 1199
BclI TGATCA 2 cut(s) 252, 432
BfaI CTAG 3 cut(s) 698, 797, 1358
BfmI CTRYAG 1 cut(s) 1053
BfuAI ACCTGC 1 cut(s) 514
BfuI GTATCC 1 cut(s) 1199
BisI GCNGC 4 cut(s) 324, 588, 801, 813
BlpI GCTNAGC 1 cut(s) 38
BlsI GCNGC 4 cut(s) 325, 589, 802, 814
BmcAI AGTACT 1 cut(s) 444
Bme1390I CCNGG 3 cut(s) 167, 746, 1110
Bme18I GGWCC 1 cut(s) 1277
BmeRI GACNNNNNGTC 1 cut(s) 1437
BmgT120I GGNCC 2 cut(s) 1078, 1277
BmiI GGNNCC 5 cut(s) 732, 997, 1278, 1318, 1363
BmrFI CCNGG 3 cut(s) 167, 746, 1110
BmsI GCATC 2 cut(s) 682, 1239
BpiI GAAGAC 2 cut(s) 168, 639
BpmI CTGGAG 2 cut(s) 975, 1200
Bpu10I CCTNAGC 2 cut(s) 60, 862
Bpu1102I GCTNAGC 1 cut(s) 38
BpuEI CTTGAG 1 cut(s) 264
BsaHI GRCGYC 1 cut(s) 1418
BsaJI CCNNGG 2 cut(s) 591, 1108
BsaWI WCCGGW 2 cut(s) 1070, 1512
BsaXI ACNNNNNCTCC 4 cut(s) 349, 379, 663, 693
Bsc4I CCNNNNNNNGG 1 cut(s) 394
Bse118I RCCGGY 1 cut(s) 1070
Bse1I ACTGG 3 cut(s) 450, 1183, 1520
BseAI TCCGGA 1 cut(s) 1512
BseBI CCWGG 3 cut(s) 167, 746, 1110
BseDI CCNNGG 2 cut(s) 591, 1108
BseGI GGATG 4 cut(s) 328, 454, 732, 1230
BseLI CCNNNNNNNGG 1 cut(s) 394
BseMII CTCAG 3 cut(s) 74, 876, 1298
BseNI ACTGG 3 cut(s) 450, 1183, 1520
BseXI GCAGC 3 cut(s) 599, 787, 799
BseYI CCCAGC 3 cut(s) 464, 1273, 1382
BsgI GTGCAG 2 cut(s) 507, 701
Bsh1236I CGCG 1 cut(s) 1196
Bsh1285I CGRYCG 1 cut(s) 1104
BshFI GGCC 1 cut(s) 1080
BshNI GGYRCC 2 cut(s) 730, 1316
BshTI ACCGGT 1 cut(s) 1070
BsiEI CGRYCG 1 cut(s) 1104
BsiHKAI GWGCWC 2 cut(s) 571, 1217
BsiSI CCGG 2 cut(s) 1071, 1513
BslFI GGGAC 2 cut(s) 68, 1290
BslI CCNNNNNNNGG 1 cut(s) 394
BsmFI GGGAC 2 cut(s) 68, 1290
BsmI GAATGC 3 cut(s) 551, 691, 1466
BsnI GGCC 1 cut(s) 1080
Bsp1286I GDGCHC 2 cut(s) 571, 1217
Bsp13I TCCGGA 1 cut(s) 1512
Bsp143I GATC 3 cut(s) 252, 432, 995
Bsp1720I GCTNAGC 1 cut(s) 38
Bsp19I CCATGG 1 cut(s) 591
BspACI CCGC 5 cut(s) 323, 395, 453, 620, 1076
BspANI GGCC 1 cut(s) 1080
BspCNI CTCAG 3 cut(s) 73, 875, 1297
BspEI TCCGGA 1 cut(s) 1512
BspFNI CGCG 1 cut(s) 1196
BspLI GGNNCC 5 cut(s) 732, 997, 1278, 1318, 1363
BspMAI CTGCAG 1 cut(s) 1057
BspMI ACCTGC 1 cut(s) 514
BspPI GGATC 2 cut(s) 990, 1003
BspT107I GGYRCC 2 cut(s) 730, 1316
BsrFI RCCGGY 1 cut(s) 1070
BsrI ACTGG 3 cut(s) 450, 1183, 1520
BssAI RCCGGY 1 cut(s) 1070
BssECI CCNNGG 2 cut(s) 591, 1108
BssMI GATC 3 cut(s) 252, 432, 995
BssNI GRCGYC 1 cut(s) 1418
BssT1I CCWWGG 1 cut(s) 591
Bst2UI CCWGG 3 cut(s) 167, 746, 1110
Bst4CI ACNGT 5 cut(s) 250, 573, 896, 1303, 1375
BstACI GRCGYC 1 cut(s) 1418
BstAPI GCANNNNNTGC 2 cut(s) 737, 977
BstC8I GCNNGC 8 cut(s) 172, 176, 208, 230, 658, 835, 1078, 1388
BstDEI CTNAG 5 cut(s) 38, 60, 862, 1063, 1284
BstDSI CCRYGG 1 cut(s) 591
BstF5I GGATG 4 cut(s) 328, 454, 732, 1230
BstFNI CGCG 1 cut(s) 1196
BstHHI GCGC 3 cut(s) 697, 989, 1198
BstKTI GATC 3 cut(s) 255, 435, 998
BstMBI GATC 3 cut(s) 252, 432, 995
BstMCI CGRYCG 1 cut(s) 1104
BstMWI GCNNNNNNNGC 9 cut(s) 593, 662, 671, 737, 746, 809, 977, 986, 1073
BstNI CCWGG 3 cut(s) 167, 746, 1110
BstSCI CCNGG 3 cut(s) 165, 744, 1108
BstSFI CTRYAG 1 cut(s) 1053
BstUI CGCG 1 cut(s) 1196
BstV1I GCAGC 3 cut(s) 599, 787, 799
BstV2I GAAGAC 2 cut(s) 168, 639
BstX2I RGATCY 1 cut(s) 995
BstXI CCANNNNNNTGG 1 cut(s) 376
BstYI RGATCY 1 cut(s) 995
BsuI GTATCC 1 cut(s) 1199
BsuRI GGCC 1 cut(s) 1080
BtgI CCRYGG 1 cut(s) 591
BtgZI GCGATG 1 cut(s) 311
BtsCI GGATG 4 cut(s) 328, 454, 732, 1230
BtsIMutI CAGTG 3 cut(s) 262, 487, 1434
BveI ACCTGC 1 cut(s) 514
Cac8I GCNNGC 8 cut(s) 172, 176, 208, 230, 658, 835, 1078, 1388
CaiI CAGNNNCTG 2 cut(s) 977, 1526
CfoI GCGC 3 cut(s) 697, 989, 1198
Cfr10I RCCGGY 1 cut(s) 1070
Cfr13I GGNCC 2 cut(s) 1078, 1277
CseI GACGC 1 cut(s) 1407
Csp6I GTAC 2 cut(s) 443, 1443
CspAI ACCGGT 1 cut(s) 1070
CviAII CATG 8 cut(s) 34, 50, 122, 592, 641, 1082, 1367, 1488
CviQI GTAC 2 cut(s) 443, 1443
DdeI CTNAG 5 cut(s) 38, 60, 862, 1063, 1284
DpnI GATC 3 cut(s) 254, 434, 997
DpnII GATC 3 cut(s) 252, 432, 995
DriI GACNNNNNGTC 1 cut(s) 1437
Eam1105I GACNNNNNGTC 1 cut(s) 1437
EciI GGCGGA 1 cut(s) 468
Eco130I CCWWGG 1 cut(s) 591
Eco32I GATATC 2 cut(s) 793, 1060
Eco47I GGWCC 1 cut(s) 1277
Eco57I CTGAAG 3 cut(s) 1031, 1257, 1431
EcoRI GAATTC 1 cut(s) 492
EcoRII CCWGG 3 cut(s) 165, 744, 1108
EcoRV GATATC 2 cut(s) 793, 1060
EcoT14I CCWWGG 1 cut(s) 591
ErhI CCWWGG 1 cut(s) 591
FaeI CATG 8 cut(s) 37, 53, 125, 595, 644, 1085, 1370, 1491
FaqI GGGAC 2 cut(s) 68, 1290
FatI CATG 8 cut(s) 33, 49, 121, 591, 640, 1081, 1366, 1487
FauI CCCGC 2 cut(s) 388, 1069
FbaI TGATCA 2 cut(s) 252, 432
Fnu4HI GCNGC 4 cut(s) 324, 588, 801, 813
FokI GGATG 4 cut(s) 315, 461, 739, 1217
Fsp4HI GCNGC 4 cut(s) 324, 588, 801, 813
FspBI CTAG 3 cut(s) 698, 797, 1358
GlaI GCGC 3 cut(s) 696, 988, 1197
GluI GCNGC 4 cut(s) 324, 588, 801, 813
GsaI CCCAGC 3 cut(s) 468, 1277, 1386
GsuI CTGGAG 2 cut(s) 975, 1200
HaeIII GGCC 1 cut(s) 1080
HapII CCGG 2 cut(s) 1071, 1513
HgaI GACGC 1 cut(s) 1407
HhaI GCGC 3 cut(s) 697, 989, 1198
Hin1I GRCGYC 1 cut(s) 1418
Hin1II CATG 8 cut(s) 37, 53, 125, 595, 644, 1085, 1370, 1491
Hin6I GCGC 3 cut(s) 695, 987, 1196
HinP1I GCGC 3 cut(s) 695, 987, 1196
HindIII AAGCTT 1 cut(s) 869
HinfI GANTC 6 cut(s) 10, 410, 899, 974, 1182, 1516
HpaII CCGG 2 cut(s) 1071, 1513
HphI GGTGA 3 cut(s) 734, 908, 1417
Hpy166II GTNNAC 2 cut(s) 820, 1408
Hpy188I TCNGA 5 cut(s) 214, 351, 544, 1050, 1287
Hpy188III TCNNGA 7 cut(s) 62, 281, 332, 782, 821, 1449, 1513
Hpy8I GTNNAC 2 cut(s) 820, 1408
Hpy99I CGWCG 1 cut(s) 351
HpyAV CCTTC 8 cut(s) 418, 483, 595, 936, 986, 1232, 1266, 1408
HpyCH4III ACNGT 5 cut(s) 250, 573, 896, 1303, 1375
HpyCH4IV ACGT 1 cut(s) 1506
HpyCH4V TGCA 9 cut(s) 130, 488, 549, 587, 682, 980, 1055, 1352, 1466
HpyF10VI GCNNNNNNNGC 9 cut(s) 593, 662, 671, 737, 746, 809, 977, 986, 1073
HpyF3I CTNAG 5 cut(s) 38, 60, 862, 1063, 1284
HpySE526I ACGT 1 cut(s) 1506
Hsp92I GRCGYC 1 cut(s) 1418
Hsp92II CATG 8 cut(s) 37, 53, 125, 595, 644, 1085, 1370, 1491
HspAI GCGC 3 cut(s) 695, 987, 1196
Kpn2I TCCGGA 1 cut(s) 1512
Ksp22I TGATCA 2 cut(s) 252, 432
Kzo9I GATC 3 cut(s) 252, 432, 995
LmnI GCTCC 4 cut(s) 566, 605, 671, 830
Lsp1109I GCAGC 3 cut(s) 599, 787, 799
LweI GCATC 2 cut(s) 682, 1239
MaeI CTAG 3 cut(s) 698, 797, 1358
MaeII ACGT 1 cut(s) 1506
MaeIII GTNAC 4 cut(s) 527, 1303, 1369, 1429
MalI GATC 3 cut(s) 254, 434, 997
MboI GATC 3 cut(s) 252, 432, 995
MboII GAAGA 2 cut(s) 173, 644
MflI RGATCY 1 cut(s) 995
MhlI GDGCHC 2 cut(s) 571, 1217
MluCI AATT 5 cut(s) 100, 399, 492, 557, 561
MlyI GAGTC 4 cut(s) 404, 983, 1191, 1525
MmeI TCCRAC 2 cut(s) 329, 808
MroI TCCGGA 1 cut(s) 1512
MroXI GAANNNNTTC 2 cut(s) 920, 1044
MseI TTAA 3 cut(s) 384, 560, 1001
MspA1I CMGCKG 1 cut(s) 665
MspI CCGG 2 cut(s) 1071, 1513
MspR9I CCNGG 3 cut(s) 167, 746, 1110
Mva1269I GAATGC 3 cut(s) 551, 691, 1466
MvaI CCWGG 3 cut(s) 167, 746, 1110
MvnI CGCG 1 cut(s) 1196
MwoI GCNNNNNNNGC 9 cut(s) 593, 662, 671, 737, 746, 809, 977, 986, 1073
NcoI CCATGG 1 cut(s) 591
NdeII GATC 3 cut(s) 252, 432, 995
NlaIII CATG 8 cut(s) 37, 53, 125, 595, 644, 1085, 1370, 1491
NlaIV GGNNCC 5 cut(s) 732, 997, 1278, 1318, 1363
NmuCI GTSAC 4 cut(s) 527, 1303, 1369, 1429
PaqCI CACCTGC 1 cut(s) 514
PcsI WCGNNNNNNNCGW 1 cut(s) 343
PctI GAATGC 3 cut(s) 551, 691, 1466
PdmI GAANNNNTTC 2 cut(s) 920, 1044
PfeI GAWTC 2 cut(s) 10, 899
PinAI ACCGGT 1 cut(s) 1070
PkrI GCNGC 4 cut(s) 325, 589, 802, 814
PleI GAGTC 4 cut(s) 404, 982, 1190, 1524
PpsI GAGTC 4 cut(s) 404, 982, 1190, 1524
PshBI ATTAAT 1 cut(s) 560
PsiI TTATAA 1 cut(s) 1484
Psp6I CCWGG 3 cut(s) 165, 744, 1108
PspFI CCCAGC 3 cut(s) 464, 1273, 1382
PspGI CCWGG 3 cut(s) 165, 744, 1108
PspN4I GGNNCC 5 cut(s) 732, 997, 1278, 1318, 1363
PspPI GGNCC 2 cut(s) 1078, 1277
PstI CTGCAG 1 cut(s) 1057
PstNI CAGNNNCTG 2 cut(s) 977, 1526
PsuI RGATCY 1 cut(s) 995
PvuII CAGCTG 1 cut(s) 665
RsaI GTAC 2 cut(s) 444, 1444
RsaNI GTAC 2 cut(s) 443, 1443
SaqAI TTAA 3 cut(s) 384, 560, 1001
SatI GCNGC 4 cut(s) 324, 588, 801, 813
Sau3AI GATC 3 cut(s) 252, 432, 995
Sau96I GGNCC 2 cut(s) 1078, 1277
ScaI AGTACT 1 cut(s) 444
SchI GAGTC 4 cut(s) 404, 983, 1191, 1525
ScrFI CCNGG 3 cut(s) 167, 746, 1110
SduI GDGCHC 2 cut(s) 571, 1217
SfaNI GCATC 2 cut(s) 682, 1239
SfcI CTRYAG 1 cut(s) 1053
SgrAI CRCCGGYG 1 cut(s) 1070
SinI GGWCC 1 cut(s) 1277
SmlI CTYRAG 1 cut(s) 279
SmoI CTYRAG 1 cut(s) 279
Sse9I AATT 5 cut(s) 100, 399, 492, 557, 561
SsiI CCGC 5 cut(s) 323, 395, 453, 620, 1076
SspMI CTAG 3 cut(s) 698, 797, 1358
StyD4I CCNGG 3 cut(s) 165, 744, 1108
StyI CCWWGG 1 cut(s) 591
TaaI ACNGT 5 cut(s) 250, 573, 896, 1303, 1375
TaiI ACGT 1 cut(s) 1509
TaqI TCGA 1 cut(s) 843
TaqII GACCGA 2 cut(s) 1118, 1327
TasI AATT 5 cut(s) 100, 399, 492, 557, 561
TatI WGTACW 1 cut(s) 442
TauI GCSGC 1 cut(s) 326
TfiI GAWTC 2 cut(s) 10, 899
Tru1I TTAA 3 cut(s) 384, 560, 1001
Tru9I TTAA 3 cut(s) 384, 560, 1001
TscAI CASTG 3 cut(s) 262, 487, 1434
TseFI GTSAC 4 cut(s) 527, 1303, 1369, 1429
TseI GCWGC 3 cut(s) 587, 800, 812
Tsp45I GTSAC 4 cut(s) 527, 1303, 1369, 1429
TspDTI ATGAA 6 cut(s) 22, 323, 465, 645, 1053, 1248
TspGWI ACGGA 2 cut(s) 651, 1042
TspRI CASTG 3 cut(s) 262, 487, 1434
VpaK11BI GGWCC 1 cut(s) 1277
VspI ATTAAT 1 cut(s) 560
XapI RAATTY 2 cut(s) 399, 492
XcmI CCANNNNNNNNNTGG 1 cut(s) 510
XmnI GAANNNNTTC 2 cut(s) 920, 1044
XspI CTAG 3 cut(s) 698, 797, 1358
ZrmI AGTACT 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.