Rh7CG194000

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0000302
Physical Location & Seq
Reverse (-)
17075608 .. 17076570
963 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG194000.1

Sequence Viewer

Length: 963 bp
ATGAAAAGAGCTGGTGGTGTTGCCATGATTCTTGTCAACCCAGACTTCGCTGGCTATACCACCATAGCAGACGCTCACGTGCTTCCGGCAACATATGTGAGTTACTCTGTAGGGGTGAGCATCAAAACCTATATAAATTCAACCTCAACTCCTACAGCTACAATCTTATTCAAAGGCACTGTTATCGGCGATCAGCTTGCTCCCAAGGTTGCTTTCTTTTCATCAAGAGGACCCAGCCTGGCAAGCCCTGCAATTTTGAAACCTGACATTATTGGTCCCGGTGTGAGCATCCTAGCTGCGTGGCCTTTTTCGGTGGATAATGCCACAGAATCTAAGGCCACATTTGACATTATTTCAGGTACTTCAATGTCATGCCCTCACCTAAGTGGCATTGTAGCCTTGCTCAAGAGCTCACACCCTGACTGGTCACCTGCTGCCATTAAGTCTGCAATGATCACAACTGCTGAAGTAAACAACCTCGTTGGCTCGGCCATTCTTGATCAAACACTTCTTGCAGCAGACCTCTTTGCCATTGGCGCATGCCATGTTAACCCTTCAAAAGCAAATGACCCTGGGCTCATCTACGACATACAACCAGAGGACTACATTCCTTACTTGTGTGGTTTGAATTACACAAGCAAACAGATAGCGGCAATCACCCAACAAAAAGTGTCGTGCTCTAAAGTAGGAGTCATACCAGAAGCACAGCTAAACTATCCCACATTCTGTATCTATATAGAGTCTGGTGGTGAGCCTCAGAATTACCCAAGAACCTTGACCAATGTTGGCCCAGCTAATTCAACTTACAAATTGGCTCCCCTGAGACTACATAAAATGGACATCACTTTGCTACCTGAGGTGCTTACATTTATAGAGGTTAACCAGAAATTGACATACCGTGTGGTATTTGCGCACAAGATGGAGCTGGGAAAGATGGTATACCATTTTCTCAGGGATATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

320

Amino Acids

34.37

Weight (kDa)

7.03

Isoelectric Point (pI)

38.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 65 - 158 7.6e-18 Subtilase family
fn3_6 PF17766 236 - 307 4.4e-13 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 439
Acc16I TGCGCA 1 cut(s) 912
Acc36I ACCTGC 1 cut(s) 439
AccI GTMKAC 1 cut(s) 939
AciI CCGC 1 cut(s) 650
AcoI YGGCCR 1 cut(s) 489
AcsI RAATTY 1 cut(s) 136
AcuI CTGAAG 1 cut(s) 486
AcvI CACGTG 1 cut(s) 79
AfaI GTAC 1 cut(s) 361
AgsI TTSAA 7 cut(s) 141, 172, 259, 366, 558, 628, 801
AjnI CCWGG 2 cut(s) 237, 571
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
AleI CACNNNNGTG 1 cut(s) 384
AluBI AGCT 8 cut(s) 11, 158, 196, 296, 411, 709, 794, 925
AluI AGCT 8 cut(s) 11, 158, 196, 296, 411, 709, 794, 925
Alw21I GWGCWC 2 cut(s) 413, 680
Alw26I GTCTC 1 cut(s) 817
AoxI GGCC 4 cut(s) 302, 336, 489, 787
ApeKI GCWGC 3 cut(s) 296, 434, 515
ApoI RAATTY 1 cut(s) 136
AspLEI GCGC 2 cut(s) 539, 913
AspS9I GGNCC 3 cut(s) 230, 275, 788
AsuC2I CCSGG 1 cut(s) 279
AsuHPI GGTGA 5 cut(s) 127, 371, 420, 649, 761
AvaII GGWCC 2 cut(s) 230, 275
AxyI CCTNAGG 1 cut(s) 855
BanII GRGCYC 2 cut(s) 413, 579
BbrPI CACGTG 1 cut(s) 79
Bbv12I GWGCWC 2 cut(s) 413, 680
BbvI GCAGC 3 cut(s) 283, 421, 527
BccI CCATC 2 cut(s) 913, 928
BcgI CGANNNNNNTGC 4 cut(s) 166, 179, 200, 213
BciT130I CCWGG 2 cut(s) 239, 573
BclI TGATCA 2 cut(s) 453, 499
BcnI CCSGG 1 cut(s) 279
BcoDI GTCTC 1 cut(s) 817
BfaI CTAG 1 cut(s) 293
BfmI CTRYAG 2 cut(s) 108, 153
BfuAI ACCTGC 1 cut(s) 439
BisI GCNGC 4 cut(s) 297, 435, 516, 651
BlsI GCNGC 4 cut(s) 298, 436, 517, 652
Bme1390I CCNGG 3 cut(s) 239, 279, 573
Bme18I GGWCC 2 cut(s) 230, 275
BmgT120I GGNCC 3 cut(s) 230, 275, 788
BmiI GGNNCC 3 cut(s) 232, 277, 816
BmrFI CCNGG 3 cut(s) 239, 279, 573
BmsI GCATC 2 cut(s) 129, 297
BpuEI CTTGAG 1 cut(s) 389
BpuMI CCSGG 1 cut(s) 279
BsaAI YACGTR 1 cut(s) 79
BsaJI CCNNGG 3 cut(s) 204, 571, 572
BsaXI ACNNNNNCTCC 2 cut(s) 133, 163
Bse1I ACTGG 1 cut(s) 428
Bse21I CCTNAGG 1 cut(s) 855
Bse3DI GCAATG 1 cut(s) 456
BseBI CCWGG 2 cut(s) 239, 573
BseDI CCNNGG 3 cut(s) 204, 571, 572
BseGI GGATG 1 cut(s) 288
BseMI GCAATG 1 cut(s) 456
BseMII CTCAG 3 cut(s) 770, 812, 846
BseNI ACTGG 1 cut(s) 428
BseXI GCAGC 3 cut(s) 283, 421, 527
BseYI CCCAGC 3 cut(s) 233, 790, 925
BshFI GGCC 4 cut(s) 304, 338, 491, 789
BsiHKAI GWGCWC 2 cut(s) 413, 680
BsiSI CCGG 2 cut(s) 86, 279
BslFI GGGAC 1 cut(s) 261
BsmAI GTCTC 1 cut(s) 817
BsmFI GGGAC 1 cut(s) 261
BsnI GGCC 4 cut(s) 304, 338, 491, 789
Bsp1286I GDGCHC 3 cut(s) 413, 579, 680
Bsp143I GATC 3 cut(s) 190, 453, 499
BspACI CCGC 1 cut(s) 650
BspANI GGCC 4 cut(s) 304, 338, 491, 789
BspCNI CTCAG 4 cut(s) 769, 813, 847, 963
BspLI GGNNCC 3 cut(s) 232, 277, 816
BspMI ACCTGC 1 cut(s) 439
BsrDI GCAATG 1 cut(s) 456
BsrI ACTGG 1 cut(s) 428
BssECI CCNNGG 3 cut(s) 204, 571, 572
BssMI GATC 3 cut(s) 190, 453, 499
BssNAI GTATAC 1 cut(s) 940
BssT1I CCWWGG 1 cut(s) 204
Bst1107I GTATAC 1 cut(s) 940
Bst2UI CCWGG 2 cut(s) 239, 573
Bst4CI ACNGT 2 cut(s) 181, 899
BstAPI GCANNNNNTGC 1 cut(s) 248
BstBAI YACGTR 1 cut(s) 79
BstC8I GCNNGC 4 cut(s) 52, 198, 244, 541
BstDEI CTNAG 6 cut(s) 333, 383, 756, 821, 855, 950
BstEII GGTNACC 1 cut(s) 426
BstF5I GGATG 1 cut(s) 288
BstHHI GCGC 2 cut(s) 539, 913
BstKTI GATC 3 cut(s) 193, 456, 502
BstMAI GTCTC 1 cut(s) 817
BstMBI GATC 3 cut(s) 190, 453, 499
BstMWI GCNNNNNNNGC 3 cut(s) 243, 248, 536
BstNI CCWGG 2 cut(s) 239, 573
BstNSI RCATGY 1 cut(s) 543
BstPI GGTNACC 1 cut(s) 426
BstSCI CCNGG 3 cut(s) 237, 277, 571
BstSFI CTRYAG 2 cut(s) 108, 153
BstV1I GCAGC 3 cut(s) 283, 421, 527
BstZ17I GTATAC 1 cut(s) 940
Bsu36I CCTNAGG 1 cut(s) 855
BsuRI GGCC 4 cut(s) 304, 338, 491, 789
BtsCI GGATG 1 cut(s) 288
BtsIMutI CAGTG 1 cut(s) 177
BveI ACCTGC 1 cut(s) 439
Cac8I GCNNGC 4 cut(s) 52, 198, 244, 541
CfoI GCGC 2 cut(s) 539, 913
Cfr13I GGNCC 3 cut(s) 230, 275, 788
CseI GACGC 1 cut(s) 80
Csp6I GTAC 1 cut(s) 360
CviAII CATG 4 cut(s) 25, 372, 540, 545
CviQI GTAC 1 cut(s) 360
DdeI CTNAG 6 cut(s) 333, 383, 756, 821, 855, 950
DpnI GATC 3 cut(s) 192, 455, 501
DpnII GATC 3 cut(s) 190, 453, 499
EaeI YGGCCR 1 cut(s) 489
Ecl136II GAGCTC 1 cut(s) 411
Eco130I CCWWGG 1 cut(s) 204
Eco24I GRGCYC 2 cut(s) 413, 579
Eco47I GGWCC 2 cut(s) 230, 275
Eco53kI GAGCTC 1 cut(s) 411
Eco57I CTGAAG 1 cut(s) 486
Eco72I CACGTG 1 cut(s) 79
Eco81I CCTNAGG 1 cut(s) 855
Eco91I GGTNACC 1 cut(s) 426
EcoICRI GAGCTC 1 cut(s) 411
EcoO109I RGGNCCY 1 cut(s) 230
EcoO65I GGTNACC 1 cut(s) 426
EcoRII CCWGG 2 cut(s) 237, 571
EcoT14I CCWWGG 1 cut(s) 204
EcoT38I GRGCYC 2 cut(s) 413, 579
ErhI CCWWGG 1 cut(s) 204
FaeI CATG 4 cut(s) 28, 375, 543, 548
FaqI GGGAC 1 cut(s) 261
FatI CATG 4 cut(s) 24, 371, 539, 544
FauNDI CATATG 1 cut(s) 94
FbaI TGATCA 2 cut(s) 453, 499
FblI GTMKAC 1 cut(s) 939
Fnu4HI GCNGC 4 cut(s) 297, 435, 516, 651
FokI GGATG 1 cut(s) 275
FriOI GRGCYC 2 cut(s) 413, 579
Fsp4HI GCNGC 4 cut(s) 297, 435, 516, 651
FspBI CTAG 1 cut(s) 293
FspI TGCGCA 1 cut(s) 912
GlaI GCGC 2 cut(s) 538, 912
GluI GCNGC 4 cut(s) 297, 435, 516, 651
GsaI CCCAGC 3 cut(s) 237, 794, 929
HaeIII GGCC 4 cut(s) 304, 338, 491, 789
HapII CCGG 2 cut(s) 86, 279
HgaI GACGC 1 cut(s) 80
HhaI GCGC 2 cut(s) 539, 913
Hin1II CATG 4 cut(s) 28, 375, 543, 548
Hin6I GCGC 2 cut(s) 537, 911
HinP1I GCGC 2 cut(s) 537, 911
HincII GTYRAC 3 cut(s) 37, 550, 880
HindII GTYRAC 3 cut(s) 37, 550, 880
HinfI GANTC 4 cut(s) 28, 329, 690, 740
HpaI GTTAAC 2 cut(s) 550, 880
HpaII CCGG 2 cut(s) 86, 279
HphI GGTGA 5 cut(s) 127, 371, 420, 649, 761
Hpy166II GTNNAC 5 cut(s) 37, 472, 550, 880, 940
Hpy188I TCNGA 1 cut(s) 759
Hpy188III TCNNGA 3 cut(s) 225, 406, 497
Hpy8I GTNNAC 5 cut(s) 37, 472, 550, 880, 940
HpyAV CCTTC 1 cut(s) 564
HpyCH4III ACNGT 2 cut(s) 181, 899
HpyCH4IV ACGT 1 cut(s) 78
HpyCH4V TGCA 3 cut(s) 251, 449, 515
HpyF10VI GCNNNNNNNGC 3 cut(s) 243, 248, 536
HpyF3I CTNAG 6 cut(s) 333, 383, 756, 821, 855, 950
HpySE526I ACGT 1 cut(s) 78
Hsp92II CATG 4 cut(s) 28, 375, 543, 548
HspAI GCGC 2 cut(s) 537, 911
Ksp22I TGATCA 2 cut(s) 453, 499
KspAI GTTAAC 2 cut(s) 550, 880
Kzo9I GATC 3 cut(s) 190, 453, 499
LmnI GCTCC 3 cut(s) 205, 820, 922
Lsp1109I GCAGC 3 cut(s) 283, 421, 527
LweI GCATC 2 cut(s) 129, 297
MaeI CTAG 1 cut(s) 293
MaeII ACGT 1 cut(s) 78
MaeIII GTNAC 2 cut(s) 101, 426
MalI GATC 3 cut(s) 192, 455, 501
MboI GATC 3 cut(s) 190, 453, 499
MhlI GDGCHC 3 cut(s) 413, 579, 680
MluCI AATT 7 cut(s) 136, 252, 628, 760, 796, 809, 887
MlyI GAGTC 2 cut(s) 699, 749
MnlI CCTC 9 cut(s) 154, 221, 387, 488, 533, 592, 765, 850, 868
MseI TTAA 3 cut(s) 441, 549, 879
MslI CAYNNNNRTG 1 cut(s) 384
MspI CCGG 2 cut(s) 86, 279
MspR9I CCNGG 3 cut(s) 239, 279, 573
MvaI CCWGG 2 cut(s) 239, 573
MwoI GCNNNNNNNGC 3 cut(s) 243, 248, 536
NciI CCSGG 1 cut(s) 279
NdeI CATATG 1 cut(s) 94
NdeII GATC 3 cut(s) 190, 453, 499
NlaIII CATG 4 cut(s) 28, 375, 543, 548
NlaIV GGNNCC 3 cut(s) 232, 277, 816
NmeAIII GCCGAG 1 cut(s) 467
NmuCI GTSAC 1 cut(s) 426
NsbI TGCGCA 1 cut(s) 912
NspI RCATGY 1 cut(s) 543
OliI CACNNNNGTG 1 cut(s) 384
PaeI GCATGC 1 cut(s) 543
PaqCI CACCTGC 1 cut(s) 439
PasI CCCWGGG 1 cut(s) 572
PfeI GAWTC 2 cut(s) 28, 329
PkrI GCNGC 4 cut(s) 298, 436, 517, 652
PleI GAGTC 2 cut(s) 698, 748
PmaCI CACGTG 1 cut(s) 79
PmlI CACGTG 1 cut(s) 79
PpsI GAGTC 2 cut(s) 698, 748
Ppu21I YACGTR 1 cut(s) 79
PpuMI RGGWCCY 1 cut(s) 230
Psp124BI GAGCTC 1 cut(s) 413
Psp5II RGGWCCY 1 cut(s) 230
Psp6I CCWGG 2 cut(s) 237, 571
PspCI CACGTG 1 cut(s) 79
PspEI GGTNACC 1 cut(s) 426
PspFI CCCAGC 3 cut(s) 233, 790, 925
PspGI CCWGG 2 cut(s) 237, 571
PspN4I GGNNCC 3 cut(s) 232, 277, 816
PspPI GGNCC 3 cut(s) 230, 275, 788
PspPPI RGGWCCY 1 cut(s) 230
RsaI GTAC 1 cut(s) 361
RsaNI GTAC 1 cut(s) 360
RseI CAYNNNNRTG 1 cut(s) 384
SacI GAGCTC 1 cut(s) 413
SaqAI TTAA 3 cut(s) 441, 549, 879
SatI GCNGC 4 cut(s) 297, 435, 516, 651
Sau3AI GATC 3 cut(s) 190, 453, 499
Sau96I GGNCC 3 cut(s) 230, 275, 788
SchI GAGTC 2 cut(s) 699, 749
ScrFI CCNGG 3 cut(s) 239, 279, 573
SduI GDGCHC 3 cut(s) 413, 579, 680
SfaNI GCATC 2 cut(s) 129, 297
SfcI CTRYAG 2 cut(s) 108, 153
SinI GGWCC 2 cut(s) 230, 275
SmiMI CAYNNNNRTG 1 cut(s) 384
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
SphI GCATGC 1 cut(s) 543
Sse9I AATT 7 cut(s) 136, 252, 628, 760, 796, 809, 887
SsiI CCGC 1 cut(s) 650
SspMI CTAG 1 cut(s) 293
SstI GAGCTC 1 cut(s) 413
StyD4I CCNGG 3 cut(s) 237, 277, 571
StyI CCWWGG 1 cut(s) 204
TaaI ACNGT 2 cut(s) 181, 899
TaiI ACGT 1 cut(s) 81
TasI AATT 7 cut(s) 136, 252, 628, 760, 796, 809, 887
TauI GCSGC 1 cut(s) 653
TfiI GAWTC 2 cut(s) 28, 329
Tru1I TTAA 3 cut(s) 441, 549, 879
Tru9I TTAA 3 cut(s) 441, 549, 879
TscAI CASTG 1 cut(s) 184
TseFI GTSAC 1 cut(s) 426
TseI GCWGC 3 cut(s) 296, 434, 515
Tsp45I GTSAC 1 cut(s) 426
TspDTI ATGAA 2 cut(s) 17, 210
TspRI CASTG 1 cut(s) 184
VpaK11BI GGWCC 2 cut(s) 230, 275
XapI RAATTY 1 cut(s) 136
XceI RCATGY 1 cut(s) 543
XmiI GTMKAC 1 cut(s) 939
XspI CTAG 1 cut(s) 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.