RLG00000003744

Subtilisin-like serine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000302
Physical Location & Seq
Forward (+)
52231906 .. 52232884
979 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003744

Sequence Viewer

Length: 666 bp
ATGTCATACCCGCACCTAAGCGGCATTGCAGCCTTGATCAAAAGTTCACACCCAGACTGGTCACCTGCTACCATCAAATCCGCCATTATGACAACCGCTGATCTGCTAAACCTCAAAGGCACACCAATTGTTGATAGATCAACTACCCCAGCAGACATCTATAGCACTGGCTCAGGCCATGTCAACCCTTTAAAAGCCAATGACCCGGGGCTCATTTATGACACAAAACTAGAAGATTACATTCCTTACTTGTGTGGTCTGAACTACACAGACAATCAAATAGAGGTAATCACCCAACAAAGAGTAAACTGCTCTGAAGTAGGAATCACAGCTGAAGCGCAGCTAAGCTATCCTTCATTTTCTATCACATTAGGGTCCTATCAAACTCAGAATTACACCAGAACTGTGACAAATGTTGGCCCGGCTAATTCAACCTACAAATTGGAGATTTTGGAACCCCAGAAATGTGGTGTGAGTGTGAACATGAGTGTGACTCCTTCAGTGCTTACATTCACACAGGTCAACCAGACCATTACATACAGTGTGGAGTTTTCCACACATCTAGCTGGGCAAGATGGTTCATTTTCACAGGGATACTTGAGATGGGTTTCTGACAAGTATTCTGTATACAGCCCCATAGCTGTGATCTTTCAGTATTTGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

222

Amino Acids

24.2

Weight (kDa)

5.23

Isoelectric Point (pI)

26.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 1 - 37 1.3e-06 Subtilase family
fn3_6 PF17766 115 - 215 1.8e-23 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 73
Acc36I ACCTGC 1 cut(s) 73
AccI GTMKAC 1 cut(s) 627
AciI CCGC 4 cut(s) 11, 21, 81, 96
AcuI CTGAAG 3 cut(s) 336, 354, 483
AgsI TTSAA 1 cut(s) 432
AluBI AGCT 5 cut(s) 332, 343, 348, 566, 641
AluI AGCT 5 cut(s) 332, 343, 348, 566, 641
Ama87I CYCGRG 1 cut(s) 205
AoxI GGCC 2 cut(s) 175, 418
ApeKI GCWGC 2 cut(s) 29, 340
AspLEI GCGC 1 cut(s) 340
AspS9I GGNCC 2 cut(s) 375, 419
AsuC2I CCSGG 3 cut(s) 206, 207, 422
AsuHPI GGTGA 2 cut(s) 54, 283
AvaI CYCGRG 1 cut(s) 205
AvaII GGWCC 1 cut(s) 375
BanII GRGCYC 1 cut(s) 213
BbvI GCAGC 2 cut(s) 41, 352
BccI CCATC 3 cut(s) 80, 569, 597
BciVI GTATCC 1 cut(s) 587
BclI TGATCA 1 cut(s) 36
BcnI CCSGG 3 cut(s) 206, 207, 422
BfaI CTAG 2 cut(s) 230, 563
BfmI CTRYAG 1 cut(s) 160
BfuAI ACCTGC 1 cut(s) 73
BfuI GTATCC 1 cut(s) 587
BisI GCNGC 3 cut(s) 22, 30, 341
BlpI GCTNAGC 1 cut(s) 344
BlsI GCNGC 3 cut(s) 23, 31, 342
Bme1390I CCNGG 3 cut(s) 206, 207, 422
Bme18I GGWCC 1 cut(s) 375
BmeT110I CYCGRG 1 cut(s) 205
BmgT120I GGNCC 2 cut(s) 375, 419
BmiI GGNNCC 2 cut(s) 376, 456
BmrFI CCNGG 3 cut(s) 206, 207, 422
BplI GAGNNNNNCTC 2 cut(s) 478, 510
Bpu10I CCTNAGC 2 cut(s) 17, 172
Bpu1102I GCTNAGC 1 cut(s) 344
BpuEI CTTGAG 1 cut(s) 619
BpuMI CCSGG 3 cut(s) 206, 207, 422
BsaJI CCNNGG 2 cut(s) 205, 206
Bse1I ACTGG 2 cut(s) 62, 172
Bse3DI GCAATG 1 cut(s) 24
BseDI CCNNGG 2 cut(s) 205, 206
BseMI GCAATG 1 cut(s) 24
BseMII CTCAG 2 cut(s) 186, 401
BseNI ACTGG 2 cut(s) 62, 172
BseXI GCAGC 2 cut(s) 41, 352
BseYI CCCAGC 2 cut(s) 148, 566
BshFI GGCC 2 cut(s) 177, 420
BsiHKCI CYCGRG 1 cut(s) 205
BsiSI CCGG 2 cut(s) 206, 422
BsnI GGCC 2 cut(s) 177, 420
BsoBI CYCGRG 1 cut(s) 205
Bsp1286I GDGCHC 1 cut(s) 213
Bsp143I GATC 4 cut(s) 36, 100, 137, 645
Bsp1720I GCTNAGC 1 cut(s) 344
BspACI CCGC 4 cut(s) 11, 21, 81, 96
BspANI GGCC 2 cut(s) 177, 420
BspCNI CTCAG 2 cut(s) 185, 400
BspLI GGNNCC 2 cut(s) 376, 456
BspMI ACCTGC 1 cut(s) 73
BsrDI GCAATG 1 cut(s) 24
BsrI ACTGG 2 cut(s) 62, 172
BssECI CCNNGG 2 cut(s) 205, 206
BssMI GATC 4 cut(s) 36, 100, 137, 645
BssNAI GTATAC 1 cut(s) 628
Bst1107I GTATAC 1 cut(s) 628
Bst4CI ACNGT 2 cut(s) 406, 542
BstDEI CTNAG 4 cut(s) 17, 172, 344, 387
BstEII GGTNACC 1 cut(s) 60
BstHHI GCGC 1 cut(s) 340
BstKTI GATC 4 cut(s) 39, 103, 140, 648
BstMBI GATC 4 cut(s) 36, 100, 137, 645
BstPI GGTNACC 1 cut(s) 60
BstSCI CCNGG 3 cut(s) 204, 205, 420
BstSFI CTRYAG 1 cut(s) 160
BstV1I GCAGC 2 cut(s) 41, 352
BstXI CCANNNNNNTGG 1 cut(s) 467
BstZ17I GTATAC 1 cut(s) 628
BsuI GTATCC 1 cut(s) 587
BsuRI GGCC 2 cut(s) 177, 420
BtsIMutI CAGTG 3 cut(s) 165, 507, 547
BveI ACCTGC 1 cut(s) 73
CfoI GCGC 1 cut(s) 340
Cfr13I GGNCC 2 cut(s) 375, 419
Cfr9I CCCGGG 1 cut(s) 205
CviAII CATG 2 cut(s) 179, 484
DdeI CTNAG 4 cut(s) 17, 172, 344, 387
DpnI GATC 4 cut(s) 38, 102, 139, 647
DpnII GATC 4 cut(s) 36, 100, 137, 645
DraI TTTAAA 1 cut(s) 192
EciI GGCGGA 1 cut(s) 70
Eco24I GRGCYC 1 cut(s) 213
Eco47I GGWCC 1 cut(s) 375
Eco57I CTGAAG 3 cut(s) 336, 354, 483
Eco88I CYCGRG 1 cut(s) 205
Eco91I GGTNACC 1 cut(s) 60
EcoO109I RGGNCCY 1 cut(s) 375
EcoO65I GGTNACC 1 cut(s) 60
EcoT38I GRGCYC 1 cut(s) 213
FaeI CATG 2 cut(s) 182, 487
FaiI YATR 9 cut(s) 7, 89, 162, 180, 219, 485, 538, 628, 638
FalI AAGNNNNNCTT 2 cut(s) 337, 369
FatI CATG 2 cut(s) 178, 483
FauI CCCGC 1 cut(s) 18
FbaI TGATCA 1 cut(s) 36
FblI GTMKAC 1 cut(s) 627
Fnu4HI GCNGC 3 cut(s) 22, 30, 341
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 3 cut(s) 22, 30, 341
FspBI CTAG 2 cut(s) 230, 563
GlaI GCGC 1 cut(s) 339
GluI GCNGC 3 cut(s) 22, 30, 341
GsaI CCCAGC 2 cut(s) 152, 570
HaeIII GGCC 2 cut(s) 177, 420
HapII CCGG 2 cut(s) 206, 422
HhaI GCGC 1 cut(s) 340
Hin1II CATG 2 cut(s) 182, 487
Hin6I GCGC 1 cut(s) 338
HinP1I GCGC 1 cut(s) 338
HincII GTYRAC 2 cut(s) 184, 523
HindII GTYRAC 2 cut(s) 184, 523
HinfI GANTC 2 cut(s) 324, 493
HpaII CCGG 2 cut(s) 206, 422
HphI GGTGA 2 cut(s) 54, 283
Hpy166II GTNNAC 6 cut(s) 47, 184, 307, 481, 523, 628
Hpy188I TCNGA 4 cut(s) 261, 316, 390, 613
Hpy8I GTNNAC 6 cut(s) 47, 184, 307, 481, 523, 628
HpyAV CCTTC 2 cut(s) 363, 507
HpyCH4III ACNGT 2 cut(s) 406, 542
HpyCH4V TGCA 1 cut(s) 29
HpyF3I CTNAG 4 cut(s) 17, 172, 344, 387
Hsp92II CATG 2 cut(s) 182, 487
HspAI GCGC 1 cut(s) 338
Ksp22I TGATCA 1 cut(s) 36
Kzo9I GATC 4 cut(s) 36, 100, 137, 645
Lsp1109I GCAGC 2 cut(s) 41, 352
MaeI CTAG 2 cut(s) 230, 563
MaeIII GTNAC 3 cut(s) 60, 406, 490
MalI GATC 4 cut(s) 38, 102, 139, 647
MboI GATC 4 cut(s) 36, 100, 137, 645
MboII GAAGA 1 cut(s) 245
MfeI CAATTG 1 cut(s) 126
MhlI GDGCHC 1 cut(s) 213
MluCI AATT 4 cut(s) 126, 391, 427, 440
MlyI GAGTC 1 cut(s) 487
MnlI CCTC 2 cut(s) 122, 277
MseI TTAA 2 cut(s) 191, 664
MslI CAYNNNNRTG 2 cut(s) 488, 641
MspA1I CMGCKG 2 cut(s) 98, 332
MspI CCGG 2 cut(s) 206, 422
MspR9I CCNGG 3 cut(s) 206, 207, 422
MunI CAATTG 1 cut(s) 126
NciI CCSGG 3 cut(s) 206, 207, 422
NdeII GATC 4 cut(s) 36, 100, 137, 645
NlaIII CATG 2 cut(s) 182, 487
NlaIV GGNNCC 2 cut(s) 376, 456
NmuCI GTSAC 3 cut(s) 60, 406, 490
PaqCI CACCTGC 1 cut(s) 73
PfeI GAWTC 1 cut(s) 324
PkrI GCNGC 3 cut(s) 23, 31, 342
PleI GAGTC 1 cut(s) 487
PpsI GAGTC 1 cut(s) 487
PpuMI RGGWCCY 1 cut(s) 375
Psp5II RGGWCCY 1 cut(s) 375
PspEI GGTNACC 1 cut(s) 60
PspFI CCCAGC 2 cut(s) 148, 566
PspN4I GGNNCC 2 cut(s) 376, 456
PspPI GGNCC 2 cut(s) 375, 419
PspPPI RGGWCCY 1 cut(s) 375
PvuII CAGCTG 1 cut(s) 332
RseI CAYNNNNRTG 2 cut(s) 488, 641
SaqAI TTAA 2 cut(s) 191, 664
SatI GCNGC 3 cut(s) 22, 30, 341
Sau3AI GATC 4 cut(s) 36, 100, 137, 645
Sau96I GGNCC 2 cut(s) 375, 419
SchI GAGTC 1 cut(s) 487
ScrFI CCNGG 3 cut(s) 206, 207, 422
SduI GDGCHC 1 cut(s) 213
SfcI CTRYAG 1 cut(s) 160
SinI GGWCC 1 cut(s) 375
SmaI CCCGGG 1 cut(s) 207
SmiMI CAYNNNNRTG 2 cut(s) 488, 641
SmlI CTYRAG 1 cut(s) 598
SmoI CTYRAG 1 cut(s) 598
Sse9I AATT 4 cut(s) 126, 391, 427, 440
SsiI CCGC 4 cut(s) 11, 21, 81, 96
SspMI CTAG 2 cut(s) 230, 563
StyD4I CCNGG 3 cut(s) 204, 205, 420
TaaI ACNGT 2 cut(s) 406, 542
TasI AATT 4 cut(s) 126, 391, 427, 440
TauI GCSGC 1 cut(s) 24
TfiI GAWTC 1 cut(s) 324
Tru1I TTAA 2 cut(s) 191, 664
Tru9I TTAA 2 cut(s) 191, 664
TscAI CASTG 3 cut(s) 172, 507, 547
TseFI GTSAC 3 cut(s) 60, 406, 490
TseI GCWGC 2 cut(s) 29, 340
Tsp45I GTSAC 3 cut(s) 60, 406, 490
TspDTI ATGAA 2 cut(s) 345, 570
TspMI CCCGGG 1 cut(s) 205
TspRI CASTG 3 cut(s) 172, 507, 547
VpaK11BI GGWCC 1 cut(s) 375
XmaI CCCGGG 1 cut(s) 205
XmiI GTMKAC 1 cut(s) 627
XspI CTAG 2 cut(s) 230, 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.