pycom04g06590

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000302
Physical Location & Seq
Reverse (-)
6659797 .. 6662139
2343 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g06590.1

Sequence Viewer

Length: 2343 bp
ATGGAGAACAAAAAGGGTGCACAAATAATCTTGCATGCTTTTTATCTTCTTGGTTTAACTTCTATGTTTTTTGCATCACTAGCAGTAGCAGACTATGAAGAAGTGGTGCTGGAACAGCTAGCTGATGTGACAAATGATGAAAGAAACAGCATACAAACTTATATAGTTTGGGTTGAAAAGCCGGTCTCTCAGAAATTTTCTCCGCAATCGAATGAAGATTTAGAGAGTTGGTACCAGTCATTCTTGCCTGAAAGTATTGCAAGCTCAAACCAACAGATGAACCAGCGAATGGTTCATTCATACCGCAACATAGCAACAGGTTTTGCTGCAAAACTGACACCCGAGGAAGTGAGAGAAATGGTAACAAAAGAAGGGTTTGTCTCAGCTCGTCCACAAAGAATTCTGCCGTTGCAGACTACTCATACTCCTGACTTCTTGGGGTTGCACCAAGGGTCTGGACTTTGGGAAGCAGCAACTTACGGTAAAGGTGTAATAATTGGACTTTTAGATACCGGGATTGTACCAGATCATCCCTCGTTTAATGACGAAGGAATGCCCCCTCCTCCAGCAAAATGGAAGGGCAAGTGCGAGTTCAATGAGACATTGTGCAATAACAAGCTTATTGGTGCAAGAAATTTCATAGGCGTAGGCAAAGGCCAACCAAAACGAAGTTTTCCATTTGACATAGCTGGTCATGGAAGCCACACATCTAGCACAGCTGCCGGAAGGTTCGTGGAAGGTGCAAGCGTGTATGGGCAGGCCAATGGCACAGCTGCTGGCATGGCACCATATGCTCACTTGGCAATGTACAAAGTCTGCGAAGGCTTTGGTTGTGCCGATGCTGACATTTTTGCTGCTCTGGACGTTGCTATTGATGATGGAGTTGACGTGCTTTCCCTCTCACTTGGTGGCCCCTCACTTCCTTTCTATGATGATGTAATTGCAATTGGTGCATTTGCAGCAATTCAAAAGGGAATTTTTTTCAGCTGCGCAGGTGGAAATTCGGGTCCTTGGAATCGCACCTTATCAAATGAGGCGCCATGGATTCTCACAGTTGGAGCAAGCACCACCGACAGAATATTAACATCTGGATTGCAGATTGGAGACGAAAAACAGTACCATTTTGACGGAAAATCTTTGTCCCAGCCTGAAGATTTTGATTCAACAGTTTTGTTACCTCTCGTTGATGCAGGCTCATTTGGCAACCAATCATCTTTTTGCAAGGCAGGGTCTCTCGAAAATGTTGAAGGGAAAATAGTGTTGTGTGAGACAGGTGGAGGAGTAACAAATATTGCCAAAGGGGAAGAAGTGAAAAGAGCTGGTGGTGCAGCCATGATTCTCATGAACAAAGAGATTGATGGATTTAGCACCTTGGCTGAAGCTCACGTGCTACCAGCAACACATGTGAGTTACGCTGCAGGGTTGCAAATTAAATTGTATATAAGTTCAACCTCAACACCTAAAGGTACAATCTTGTTCAACGGCACTGTCATCGGAGATGCGCTTGCTCCCAAAGTTGCTTCCTTTTCATCAAGAGGACCAAACATTGCAAGCCCCGGAATTTTGAAACCTGATGTCATTGGACCCGGTGTTAGCATCCTAGCAGCATGGCCTTATAATGTGGACAATGTCACACATCCGAATCCCAAGGCATTATTTAACATCATTTCGGGTACCTCAATGGCCTGTCCTCACCTAAGTGGCATTGCCGCCTTGCTCAAGAGCACCCACCCTGACTGGTCACCAGCTGCTATTAAATCAGCTATCATGACTACCGCTGATGTATTAAACCTCCTAGGCTCACCCATCGTGGATGAAAGTGGCCTTAAACCTGCGGACGTCTTTGCAATTGGTGCAGGCCATGTTAACCCTTCAAAGGCGAATGACCCGGGGCTCATCTTCGACTTAAAACCAGAGGATTACATTCCTTACTTGTGTGGTTTGAATTACAATGACACAGCGATAAAGATCATCACCCAACAAGCAGTGAAATGCTCCCAAGTTGGAGCCATTCCAGAAGCACAGCTCAATTATCCTTCATTTGCTATTACAATAGGCCCCAATCAGACTCGGACTCAGTATTATACAAGGACTGTGAGGAATGTCGGGCCAGCTACTTCAACCTACAATTTGGAGCTTTTAGTACCACATGAAATGGGCATGAGTGTGAATCCTCAGGTGCTTACATTCACAGAGGTTAACCAGGAGATTACATACCATGTCGAGTTTAATGCACATGACGGCGCAGGGAAGGATGGTGTACCATTTGGTCAGGGATACTTGAGATGGTTTTCCGATAAGCATAATGTTACTACCCCTATAGCTGTGCTCTTTGACTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

781

Amino Acids

83.36

Weight (kDa)

5.27

Isoelectric Point (pI)

34.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 53 - 137 4.7e-09 Peptidase inhibitor I9
Peptidase_S8 PF00082 161 - 597 2.2e-44 Subtilase family
PA PF02225 392 - 477 7.7e-12 PA domain
fn3_6 PF17766 675 - 776 3.9e-24 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1617
AarI CACCTGC 1 cut(s) 983
AatII GACGTC 1 cut(s) 1842
Acc16I TGCGCA 1 cut(s) 991
Acc36I ACCTGC 2 cut(s) 983, 1840
Acc65I GGTACC 2 cut(s) 231, 1673
AccB1I GGYRCC 4 cut(s) 231, 784, 1036, 1673
AccB7I CCANNNNNTGG 1 cut(s) 289
AciI CCGC 5 cut(s) 203, 304, 1710, 1776, 1835
AcsI RAATTY 6 cut(s) 194, 399, 634, 975, 1000, 1560
AcuI CTGAAG 2 cut(s) 1170, 1398
AcvI CACGTG 1 cut(s) 1387
AcyI GRCGYC 2 cut(s) 1037, 1839
AdeI CACNNNGTG 1 cut(s) 908
AfaI GTAC 8 cut(s) 233, 522, 809, 1118, 1468, 1675, 2145, 2262
AfiI CCNNNNNNNGG 2 cut(s) 289, 1876
AflIII ACRYGT 1 cut(s) 1402
AjiI CACGTC 1 cut(s) 889
AjnI CCWGG 1 cut(s) 2202
AleI CACNNNNGTG 1 cut(s) 1698
Alw21I GWGCWC 3 cut(s) 22, 1727, 2331
Alw26I GTCTC 6 cut(s) 190, 385, 593, 1098, 1236, 1262
Alw44I GTGCAC 1 cut(s) 18
AlwNI CAGNNNCTG 1 cut(s) 776
Ama87I CYCGRG 2 cut(s) 341, 1888
AoxI GGCC 9 cut(s) 655, 759, 910, 1610, 1683, 1822, 1858, 2056, 2108
ApaLI GTGCAC 1 cut(s) 18
ApoI RAATTY 6 cut(s) 194, 399, 634, 975, 1000, 1560
Asp718I GGTACC 2 cut(s) 231, 1673
AspA2I CCTAGG 1 cut(s) 1795
AspLEI GCGC 4 cut(s) 992, 1039, 1504, 2246
AspS9I GGNCC 6 cut(s) 911, 1007, 1538, 1583, 2057, 2108
AsuC2I CCSGG 5 cut(s) 514, 1557, 1587, 1889, 1890
AsuHPI GGTGA 4 cut(s) 1685, 1734, 1794, 1966
AsuNHI GCTAGC 1 cut(s) 118
AvaI CYCGRG 2 cut(s) 341, 1888
AvaII GGWCC 3 cut(s) 1007, 1538, 1583
AvrII CCTAGG 1 cut(s) 1795
AxyI CCTNAGG 1 cut(s) 2175
BaeGI GKGCMC 1 cut(s) 22
BanI GGYRCC 4 cut(s) 231, 784, 1036, 1673
BanII GRGCYC 1 cut(s) 1896
BbrPI CACGTG 1 cut(s) 1387
Bbv12I GWGCWC 3 cut(s) 22, 1727, 2331
BccI CCATC 5 cut(s) 872, 1352, 1814, 2249, 2280
BceAI ACGGC 3 cut(s) 391, 1498, 2257
BcgI CGANNNNNNTGC 4 cut(s) 1474, 1508, 2213, 2247
BciT130I CCWGG 1 cut(s) 2204
BciVI GTATCC 1 cut(s) 2270
BcnI CCSGG 5 cut(s) 514, 1557, 1587, 1889, 1890
BcoDI GTCTC 6 cut(s) 190, 385, 593, 1098, 1236, 1262
BfaI CTAG 5 cut(s) 80, 119, 711, 1601, 1796
BfmI CTRYAG 2 cut(s) 1416, 2319
BfoI RGCGCY 1 cut(s) 1040
BfuAI ACCTGC 2 cut(s) 983, 1840
BfuI GTATCC 1 cut(s) 2270
BlnI CCTAGG 1 cut(s) 1795
Bme1390I CCNGG 6 cut(s) 514, 1557, 1587, 1889, 1890, 2204
Bme18I GGWCC 3 cut(s) 1007, 1538, 1583
BmeT110I CYCGRG 2 cut(s) 341, 1888
BmgBI CACGTC 1 cut(s) 889
BmgT120I GGNCC 6 cut(s) 911, 1007, 1538, 1583, 2057, 2108
BmiI GGNNCC 9 cut(s) 233, 786, 913, 1008, 1038, 1585, 1675, 2008, 2059
BmrFI CCNGG 6 cut(s) 514, 1557, 1587, 1889, 1890, 2204
BmsI GCATC 5 cut(s) 83, 829, 1177, 1489, 1605
BmtI GCTAGC 1 cut(s) 122
BpmI CTGGAG 1 cut(s) 549
BpuEI CTTGAG 2 cut(s) 1703, 2302
BpuMI CCSGG 5 cut(s) 514, 1557, 1587, 1889, 1890
BsaAI YACGTR 1 cut(s) 1387
BsaBI GATNNNNATC 1 cut(s) 1967
BsaHI GRCGYC 2 cut(s) 1037, 1839
BsaI GGTCTC 2 cut(s) 190, 1236
BsaXI ACNNNNNCTCC 4 cut(s) 409, 439, 873, 903
Bsc4I CCNNNNNNNGG 2 cut(s) 289, 1876
Bse118I RCCGGY 1 cut(s) 181
Bse1I ACTGG 2 cut(s) 235, 1742
Bse21I CCTNAGG 1 cut(s) 2175
Bse3DI GCAATG 3 cut(s) 810, 1545, 1704
Bse8I GATNNNNATC 1 cut(s) 1967
BseBI CCWGG 1 cut(s) 2204
BseGI GGATG 5 cut(s) 529, 1596, 1636, 1819, 2260
BseJI GATNNNNATC 1 cut(s) 1967
BseLI CCNNNNNNNGG 2 cut(s) 289, 1876
BseMI GCAATG 3 cut(s) 810, 1545, 1704
BseMII CTCAG 4 cut(s) 203, 396, 2090, 2189
BseNI ACTGG 2 cut(s) 235, 1742
BseRI GAGGAG 2 cut(s) 552, 1293
BseSI GKGCMC 1 cut(s) 22
BseYI CCCAGC 1 cut(s) 1143
BsgI GTGCAG 2 cut(s) 1347, 1875
BshFI GGCC 9 cut(s) 657, 761, 912, 1612, 1685, 1824, 1860, 2058, 2110
BshNI GGYRCC 4 cut(s) 231, 784, 1036, 1673
BsiHKAI GWGCWC 3 cut(s) 22, 1727, 2331
BsiHKCI CYCGRG 2 cut(s) 341, 1888
BsiSI CCGG 6 cut(s) 182, 513, 723, 1557, 1587, 1889
BslFI GGGAC 1 cut(s) 1126
BslI CCNNNNNNNGG 2 cut(s) 289, 1876
BsmAI GTCTC 6 cut(s) 190, 385, 593, 1098, 1236, 1262
BsmBI CGTCTC 1 cut(s) 1098
BsmFI GGGAC 1 cut(s) 1126
BsmI GAATGC 1 cut(s) 558
BsnI GGCC 9 cut(s) 657, 761, 912, 1612, 1685, 1824, 1860, 2058, 2110
Bso31I GGTCTC 2 cut(s) 190, 1236
BsoBI CYCGRG 2 cut(s) 341, 1888
Bsp1286I GDGCHC 4 cut(s) 22, 1727, 1896, 2331
Bsp1407I TGTACA 1 cut(s) 807
Bsp143I GATC 2 cut(s) 526, 1968
Bsp19I CCATGG 1 cut(s) 1040
BspACI CCGC 5 cut(s) 203, 304, 1710, 1776, 1835
BspANI GGCC 9 cut(s) 657, 761, 912, 1612, 1685, 1824, 1860, 2058, 2110
BspCNI CTCAG 4 cut(s) 202, 395, 2089, 2188
BspHI TCATGA 2 cut(s) 1341, 1767
BspLI GGNNCC 9 cut(s) 233, 786, 913, 1008, 1038, 1585, 1675, 2008, 2059
BspMAI CTGCAG 1 cut(s) 1420
BspMI ACCTGC 2 cut(s) 983, 1840
BspOI GCTAGC 1 cut(s) 122
BspT107I GGYRCC 4 cut(s) 231, 784, 1036, 1673
BspTNI GGTCTC 2 cut(s) 190, 1236
BsrDI GCAATG 3 cut(s) 810, 1545, 1704
BsrFI RCCGGY 1 cut(s) 181
BsrGI TGTACA 1 cut(s) 807
BsrI ACTGG 2 cut(s) 235, 1742
BssAI RCCGGY 1 cut(s) 181
BssMI GATC 2 cut(s) 526, 1968
BssNI GRCGYC 2 cut(s) 1037, 1839
BssT1I CCWWGG 6 cut(s) 448, 1010, 1040, 1371, 1647, 1795
Bst2UI CCWGG 1 cut(s) 2204
Bst4CI ACNGT 6 cut(s) 482, 1054, 1116, 1168, 1489, 2095
BstACI GRCGYC 2 cut(s) 1037, 1839
BstAPI GCANNNNNTGC 3 cut(s) 791, 950, 1853
BstAUI TGTACA 1 cut(s) 807
BstBAI YACGTR 1 cut(s) 1387
BstDEI CTNAG 5 cut(s) 189, 382, 1697, 2076, 2175
BstDSI CCRYGG 1 cut(s) 1040
BstEII GGTNACC 1 cut(s) 1740
BstF5I GGATG 5 cut(s) 529, 1596, 1636, 1819, 2260
BstH2I RGCGCY 1 cut(s) 1040
BstHHI GCGC 4 cut(s) 992, 1039, 1504, 2246
BstKTI GATC 2 cut(s) 529, 1971
BstMAI GTCTC 6 cut(s) 190, 385, 593, 1098, 1236, 1262
BstMBI GATC 2 cut(s) 526, 1968
BstNI CCWGG 1 cut(s) 2204
BstNSI RCATGY 2 cut(s) 38, 1406
BstPI GGTNACC 1 cut(s) 1740
BstSCI CCNGG 6 cut(s) 512, 1555, 1585, 1887, 1888, 2202
BstSFI CTRYAG 2 cut(s) 1416, 2319
BstSLI GKGCMC 1 cut(s) 22
BstXI CCANNNNNNTGG 2 cut(s) 455, 573
Bsu36I CCTNAGG 1 cut(s) 2175
BsuI GTATCC 1 cut(s) 2270
BsuRI GGCC 9 cut(s) 657, 761, 912, 1612, 1685, 1824, 1860, 2058, 2110
BtgI CCRYGG 1 cut(s) 1040
BtrI CACGTC 1 cut(s) 889
BtsCI GGATG 5 cut(s) 529, 1596, 1636, 1819, 2260
BtsI GCAGTG 1 cut(s) 1992
BtsIMutI CAGTG 2 cut(s) 1485, 1992
BveI ACCTGC 2 cut(s) 983, 1840
CaiI CAGNNNCTG 1 cut(s) 776
CciI TCATGA 2 cut(s) 1341, 1767
CfoI GCGC 4 cut(s) 992, 1039, 1504, 2246
Cfr10I RCCGGY 1 cut(s) 181
Cfr13I GGNCC 6 cut(s) 911, 1007, 1538, 1583, 2057, 2108
Cfr9I CCCGGG 1 cut(s) 1888
Csp6I GTAC 8 cut(s) 232, 521, 808, 1117, 1467, 1674, 2144, 2261
CviQI GTAC 8 cut(s) 232, 521, 808, 1117, 1467, 1674, 2144, 2261
DdeI CTNAG 5 cut(s) 189, 382, 1697, 2076, 2175
DinI GGCGCC 1 cut(s) 1038
DpnI GATC 2 cut(s) 528, 1970
DpnII GATC 2 cut(s) 526, 1968
DraIII CACNNNGTG 1 cut(s) 908
Eco130I CCWWGG 6 cut(s) 448, 1010, 1040, 1371, 1647, 1795
Eco24I GRGCYC 1 cut(s) 1896
Eco31I GGTCTC 2 cut(s) 190, 1236
Eco47I GGWCC 3 cut(s) 1007, 1538, 1583
Eco57I CTGAAG 2 cut(s) 1170, 1398
Eco72I CACGTG 1 cut(s) 1387
Eco81I CCTNAGG 1 cut(s) 2175
Eco88I CYCGRG 2 cut(s) 341, 1888
Eco91I GGTNACC 1 cut(s) 1740
EcoO109I RGGNCCY 2 cut(s) 1007, 2057
EcoO65I GGTNACC 1 cut(s) 1740
EcoRI GAATTC 1 cut(s) 399
EcoRII CCWGG 1 cut(s) 2202
EcoT14I CCWWGG 6 cut(s) 448, 1010, 1040, 1371, 1647, 1795
EcoT38I GRGCYC 1 cut(s) 1896
EgeI GGCGCC 1 cut(s) 1038
EheI GGCGCC 1 cut(s) 1038
ErhI CCWWGG 6 cut(s) 448, 1010, 1040, 1371, 1647, 1795
Esp3I CGTCTC 1 cut(s) 1098
FaqI GGGAC 1 cut(s) 1126
FauNDI CATATG 1 cut(s) 790
FokI GGATG 5 cut(s) 516, 1583, 1623, 1826, 2267
FriOI GRGCYC 1 cut(s) 1896
FspBI CTAG 5 cut(s) 80, 119, 711, 1601, 1796
FspI TGCGCA 1 cut(s) 991
GlaI GCGC 4 cut(s) 991, 1038, 1503, 2245
GsaI CCCAGC 1 cut(s) 1147
GsuI CTGGAG 1 cut(s) 549
HaeII RGCGCY 1 cut(s) 1040
HaeIII GGCC 9 cut(s) 657, 761, 912, 1612, 1685, 1824, 1860, 2058, 2110
HapII CCGG 6 cut(s) 182, 513, 723, 1557, 1587, 1889
HhaI GCGC 4 cut(s) 992, 1039, 1504, 2246
Hin1I GRCGYC 2 cut(s) 1037, 1839
Hin6I GCGC 4 cut(s) 990, 1037, 1502, 2244
HinP1I GCGC 4 cut(s) 990, 1037, 1502, 2244
HincII GTYRAC 3 cut(s) 886, 1867, 2200
HindII GTYRAC 3 cut(s) 886, 1867, 2200
HindIII AAGCTT 1 cut(s) 617
HinfI GANTC 9 cut(s) 1015, 1045, 1160, 1336, 1642, 2068, 2074, 2170, 2336
HpaI GTTAAC 2 cut(s) 1867, 2200
HpaII CCGG 6 cut(s) 182, 513, 723, 1557, 1587, 1889
HphI GGTGA 4 cut(s) 1685, 1734, 1794, 1966
Hpy166II GTNNAC 7 cut(s) 20, 392, 886, 1624, 1867, 2200, 2261
Hpy188I TCNGA 6 cut(s) 192, 1496, 1641, 2067, 2073, 2296
Hpy8I GTNNAC 7 cut(s) 20, 392, 886, 1624, 1867, 2200, 2261
HpyCH4III ACNGT 6 cut(s) 482, 1054, 1116, 1168, 1489, 2095
HpyCH4IV ACGT 4 cut(s) 864, 888, 1386, 1839
HpyF3I CTNAG 5 cut(s) 189, 382, 1697, 2076, 2175
HpySE526I ACGT 4 cut(s) 864, 888, 1386, 1839
Hsp92I GRCGYC 2 cut(s) 1037, 1839
HspAI GCGC 4 cut(s) 990, 1037, 1502, 2244
KasI GGCGCC 1 cut(s) 1036
KpnI GGTACC 2 cut(s) 235, 1677
KspAI GTTAAC 2 cut(s) 1867, 2200
Kzo9I GATC 2 cut(s) 526, 1968
LmnI GCTCC 5 cut(s) 1058, 1513, 2000, 2006, 2134
LweI GCATC 5 cut(s) 83, 829, 1177, 1489, 1605
MaeI CTAG 5 cut(s) 80, 119, 711, 1601, 1796
MaeII ACGT 4 cut(s) 864, 888, 1386, 1839
MaeIII GTNAC 8 cut(s) 127, 361, 1173, 1282, 1409, 1630, 1740, 2308
MalI GATC 2 cut(s) 528, 1970
MboI GATC 2 cut(s) 526, 1968
MboII GAAGA 6 cut(s) 38, 110, 227, 1163, 1316, 1891
MfeI CAATTG 2 cut(s) 945, 1848
MhlI GDGCHC 4 cut(s) 22, 1727, 1896, 2331
Mly113I GGCGCC 1 cut(s) 1037
MlyI GAGTC 3 cut(s) 2062, 2068, 2330
MmeI TCCRAC 2 cut(s) 1036, 1984
MslI CAYNNNNRTG 2 cut(s) 1698, 2165
MspA1I CMGCKG 5 cut(s) 719, 773, 987, 1748, 1778
MspI CCGG 6 cut(s) 182, 513, 723, 1557, 1587, 1889
MspR9I CCNGG 6 cut(s) 514, 1557, 1587, 1889, 1890, 2204
MunI CAATTG 2 cut(s) 945, 1848
Mva1269I GAATGC 1 cut(s) 558
MvaI CCWGG 1 cut(s) 2204
NarI GGCGCC 1 cut(s) 1037
NciI CCSGG 5 cut(s) 514, 1557, 1587, 1889, 1890
NcoI CCATGG 1 cut(s) 1040
NdeI CATATG 1 cut(s) 790
NdeII GATC 2 cut(s) 526, 1968
NheI GCTAGC 1 cut(s) 118
NlaIV GGNNCC 9 cut(s) 233, 786, 913, 1008, 1038, 1585, 1675, 2008, 2059
NmuCI GTSAC 3 cut(s) 127, 1630, 1740
NsbI TGCGCA 1 cut(s) 991
NspI RCATGY 2 cut(s) 38, 1406
OliI CACNNNNGTG 1 cut(s) 1698
PaeI GCATGC 1 cut(s) 38
PagI TCATGA 2 cut(s) 1341, 1767
PaqCI CACCTGC 1 cut(s) 983
PciI ACATGT 1 cut(s) 1402
PctI GAATGC 1 cut(s) 558
PfeI GAWTC 6 cut(s) 1015, 1045, 1160, 1336, 1642, 2170
PflFI GACNNNGTC 1 cut(s) 1628
PflMI CCANNNNNTGG 1 cut(s) 289
PleI GAGTC 3 cut(s) 2062, 2068, 2330
PluTI GGCGCC 1 cut(s) 1040
PmaCI CACGTG 1 cut(s) 1387
PmlI CACGTG 1 cut(s) 1387
PpsI GAGTC 3 cut(s) 2062, 2068, 2330
Ppu21I YACGTR 1 cut(s) 1387
PpuMI RGGWCCY 1 cut(s) 1007
PscI ACATGT 1 cut(s) 1402
PsiI TTATAA 1 cut(s) 1617
Psp5II RGGWCCY 1 cut(s) 1007
Psp6I CCWGG 1 cut(s) 2202
PspCI CACGTG 1 cut(s) 1387
PspEI GGTNACC 1 cut(s) 1740
PspFI CCCAGC 1 cut(s) 1143
PspGI CCWGG 1 cut(s) 2202
PspN4I GGNNCC 9 cut(s) 233, 786, 913, 1008, 1038, 1585, 1675, 2008, 2059
PspPI GGNCC 6 cut(s) 911, 1007, 1538, 1583, 2057, 2108
PspPPI RGGWCCY 1 cut(s) 1007
PstI CTGCAG 1 cut(s) 1420
PstNI CAGNNNCTG 1 cut(s) 776
PsyI GACNNNGTC 1 cut(s) 1628
PvuII CAGCTG 4 cut(s) 719, 773, 987, 1748
RsaI GTAC 8 cut(s) 233, 522, 809, 1118, 1468, 1675, 2145, 2262
RsaNI GTAC 8 cut(s) 232, 521, 808, 1117, 1467, 1674, 2144, 2261
RseI CAYNNNNRTG 2 cut(s) 1698, 2165
Sau3AI GATC 2 cut(s) 526, 1968
Sau96I GGNCC 6 cut(s) 911, 1007, 1538, 1583, 2057, 2108
SchI GAGTC 3 cut(s) 2062, 2068, 2330
ScrFI CCNGG 6 cut(s) 514, 1557, 1587, 1889, 1890, 2204
SduI GDGCHC 4 cut(s) 22, 1727, 1896, 2331
SfaNI GCATC 5 cut(s) 83, 829, 1177, 1489, 1605
SfcI CTRYAG 2 cut(s) 1416, 2319
SfoI GGCGCC 1 cut(s) 1038
SinI GGWCC 3 cut(s) 1007, 1538, 1583
SmaI CCCGGG 1 cut(s) 1890
SmiMI CAYNNNNRTG 2 cut(s) 1698, 2165
SmlI CTYRAG 2 cut(s) 1718, 2281
SmoI CTYRAG 2 cut(s) 1718, 2281
SphI GCATGC 1 cut(s) 38
SsiI CCGC 5 cut(s) 203, 304, 1710, 1776, 1835
SspDI GGCGCC 1 cut(s) 1036
SspI AATATT 2 cut(s) 1080, 1291
SspMI CTAG 5 cut(s) 80, 119, 711, 1601, 1796
StyD4I CCNGG 6 cut(s) 512, 1555, 1585, 1887, 1888, 2202
StyI CCWWGG 6 cut(s) 448, 1010, 1040, 1371, 1647, 1795
TaaI ACNGT 6 cut(s) 482, 1054, 1116, 1168, 1489, 2095
TaiI ACGT 4 cut(s) 867, 891, 1389, 1842
TaqI TCGA 4 cut(s) 209, 1236, 1902, 2223
TatI WGTACW 1 cut(s) 807
TauI GCSGC 1 cut(s) 1712
TfiI GAWTC 6 cut(s) 1015, 1045, 1160, 1336, 1642, 2170
TscAI CASTG 2 cut(s) 1492, 1992
TseFI GTSAC 3 cut(s) 127, 1630, 1740
Tsp45I GTSAC 3 cut(s) 127, 1630, 1740
TspGWI ACGGA 1 cut(s) 1143
TspMI CCCGGG 1 cut(s) 1888
TspRI CASTG 2 cut(s) 1492, 1992
Tth111I GACNNNGTC 1 cut(s) 1628
Van91I CCANNNNNTGG 1 cut(s) 289
VneI GTGCAC 1 cut(s) 18
VpaK11BI GGWCC 3 cut(s) 1007, 1538, 1583
XapI RAATTY 6 cut(s) 194, 399, 634, 975, 1000, 1560
XceI RCATGY 2 cut(s) 38, 1406
XmaI CCCGGG 1 cut(s) 1888
XmaJI CCTAGG 1 cut(s) 1795
XspI CTAG 5 cut(s) 80, 119, 711, 1601, 1796
ZraI GACGTC 1 cut(s) 1840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.