Prupe.5G085300_v2.0.a1

Peptidase inhibitor I9

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000302
Physical Location & Seq
Forward (+)
9733260 .. 9735197
1938 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G085300.1

Sequence Viewer

Length: 1938 bp
ATGAAGAACAAAAAGGGTATAGCATTAGTGCAGGTGCTACATCTTCTTGGTTTAACTTTCATGTTTTATTTATCAATAGCAGTTGCACAAGAGGAGCAAACTAGCTTGCAAACTTATATTGTTTGGGTTGAAAAGCCGATCTCCCAGAATTCCTATGCACAATCTCATGAAGATTTGGAGAGTTGGTATCAATCATTTTTGCCTGAAACTAGTGCAAACTCAAACCAGCTGATGAAGCAAAGGATGGTTCATGCTTACCGCCATATAGCCACGGGCTTTGCTGCAAAACTGACACAGGAGGAAGTAAAGGCAATGGAAAATAAAGAAGGGTTTGTGTCAGCTCATCTACAGAGAACTCTGCCTTTGCACACTACTCATAGTCCTGAATTCTTAGGATTACACCAAGGATTGGGACTTTGGGAACAAACAAACTATGGTGAAGGAGTGATAATTGGAGTTTTGGATACTGGGATTGGACCTGATCACCCTTCATTTAGTGATGAAGGAGTATCCCCACCTCCGGCTAAATGGAAAGGCAAATGTGTGTTCAACGGAACAGTCTGCAATAACAAGCTTATTGGTGCAAAAAATTTCATAGACGCAGGCAAAGGGAAAGCTAGAAGGAGTGCTCCATTTGACCAAGATGGCCATGGCACCCACACGTCTAGCACAGCTGCTGGAAATTTTGTGGAAGGTGCAAGCTTGTTTGGACAGGCCAATGGCACAGCATCTGGCATGGCCCCTTATGCTCACTTGGCAATTTATAAAGTCTGTGGCGGCTATGATTGCGCTGATGCTGACGTTTTGGCTGCAATGGATGCTGCTGTTGATGATGGCGTTGACGTGCTCTCCCTCTCACTTGGCGGTCCCTCAGTTCCTCTCTATGTTGATCTGATTGCCGTTGGCGCATTTGGAGCAATGCGAAAGGGAATTTTTGTCAGCTGTTCAGCTGGAAATGATGGTCCTGAACATGGTTCTTTATCAAATGAGGCTCCGTGGATTCTCACTGTTGGAGCAAGCACCACTGACCGAATATTAAACTTGACCGCAACACCTACAGCCACAAACTTGTCCAAAGTCTCCGGATCAGATCCACTCGCTCCAAAGGTGGCTATCTTTTCATCAAGAGGACCAAACATTGCAAGCCCTGGAATTCTAAAACCTGATATCATTGGACCTGGTATTGACATCCTAGCAGCATGGCCCTATTCTGTGGACAATGCCACATCTAAGGCAACATTTAACATAATTTCGGGTACGTCAATGTCATGTCCTCACCTAAGTGGCATTGCAGCCTTGCTTAAGAGCTCACACCCTGACTGGTCACCAGCTGCGATTAAATCTGCAATCATGACAACCGCGGATGTACTAAACCTCATTGGCAAACCCATTGTCGATGAATGGCTTAGGCCGGCAGACATCTTTGCAATAGGTGCAGGCCATGTTAACCCTTCAAAAGCAATTGACCCGGGCCTCATCTACGACATACAAGCAGAGGACTACATTCCTTACTTGTGTGGTTTAAACTACACAGAGAAACAAATACGTATCCTCACCCAACAAGCAGTGAACTGCTCTCAAGTTGGAGCCATAGAAGAAACACAGCTAAATTATCCTTCATTTTCTATTTTTGTGAGTTCAAGTCACAAGCTGTGGACTCAGTCTGCACATTATACAAGAACTGTCAAGAACGTTGGCCCGACTAATTCGGTTTACAGCTTGGATCTCTTAGTACCAAATAAAACGAGGATGAGTGTGAATCCTCAAGTGCTCACATTCACAGAGGTTAACCAGACCATTACGTACCAGGTGGAGTTTCTTGCACTTGACAGTGCTACTTCTGGAGGGGATGATTTTCAGTCACAGGGATACTTGAGATGGGTTTCTGATCAGCATAATGTTACTAGCCCAATAGCTGTGGTCATTGAGGCTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

646

Amino Acids

69.04

Weight (kDa)

5.66

Isoelectric Point (pI)

35.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 765
AarI CACCTGC 1 cut(s) 22
Acc36I ACCTGC 1 cut(s) 22
AccB1I GGYRCC 1 cut(s) 653
AccB7I CCANNNNNTGG 1 cut(s) 409
AccII CGCG 1 cut(s) 1361
AccIII TCCGGA 1 cut(s) 1082
AciI CCGC 6 cut(s) 259, 777, 864, 1047, 1359, 1361
AclI AACGTT 1 cut(s) 1692
AclWI GGATC 3 cut(s) 1085, 1093, 1731
AcoI YGGCCR 1 cut(s) 646
AcsI RAATTY 6 cut(s) 148, 386, 589, 682, 930, 1152
AfaI GTAC 4 cut(s) 1258, 1368, 1734, 1805
AfiI CCNNNNNNNGG 3 cut(s) 409, 520, 971
AflII CTTAAG 1 cut(s) 1301
AflIII ACRYGT 1 cut(s) 660
AgsI TTSAA 4 cut(s) 131, 550, 1455, 1641
AhlI ACTAGT 1 cut(s) 209
AjiI CACGTC 2 cut(s) 663, 844
AjnI CCWGG 3 cut(s) 1147, 1177, 1806
AleI CACNNNNGTG 1 cut(s) 1281
Alw21I GWGCWC 4 cut(s) 631, 849, 1310, 1773
Alw26I GTCTC 1 cut(s) 1084
AlwI GGATC 3 cut(s) 1085, 1093, 1731
AlwNI CAGNNNCTG 2 cut(s) 677, 731
Ama87I CYCGRG 1 cut(s) 1468
Aor13HI TCCGGA 1 cut(s) 1082
AoxI GGCC 8 cut(s) 646, 714, 738, 1202, 1409, 1438, 1471, 1696
ApeKI GCWGC 7 cut(s) 281, 674, 809, 821, 1196, 1292, 1331
ApoI RAATTY 6 cut(s) 148, 386, 589, 682, 930, 1152
ArsI GACNNNNNNTTYG 2 cut(s) 1377, 1409
AspLEI GCGC 2 cut(s) 791, 908
AspS9I GGNCC 9 cut(s) 476, 739, 866, 962, 1130, 1175, 1203, 1471, 1697
AsuC2I CCSGG 2 cut(s) 1469, 1470
AsuHPI GGTGA 5 cut(s) 449, 476, 1268, 1317, 1546
AvaI CYCGRG 1 cut(s) 1468
AvaII GGWCC 5 cut(s) 476, 866, 962, 1130, 1175
BalI TGGCCA 1 cut(s) 648
BanI GGYRCC 1 cut(s) 653
BanII GRGCYC 1 cut(s) 1310
Bbv12I GWGCWC 4 cut(s) 631, 849, 1310, 1773
BbvI GCAGC 7 cut(s) 268, 661, 796, 808, 1208, 1304, 1318
BccI CCATC 5 cut(s) 238, 638, 827, 953, 1872
BceAI ACGGC 1 cut(s) 884
BciT130I CCWGG 3 cut(s) 1149, 1179, 1808
BciVI GTATCC 4 cut(s) 457, 520, 1559, 1862
BclI TGATCA 2 cut(s) 481, 1888
BcnI CCSGG 2 cut(s) 1469, 1470
BcoDI GTCTC 1 cut(s) 1084
BcuI ACTAGT 1 cut(s) 209
BfaI CTAG 6 cut(s) 102, 210, 618, 666, 1193, 1905
BfmI CTRYAG 2 cut(s) 347, 1056
BfrI CTTAAG 1 cut(s) 1301
BfuAI ACCTGC 1 cut(s) 22
BfuI GTATCC 4 cut(s) 457, 520, 1559, 1862
BisI GCNGC 8 cut(s) 282, 675, 778, 810, 822, 1197, 1293, 1332
BlsI GCNGC 8 cut(s) 283, 676, 779, 811, 823, 1198, 1294, 1333
Bme1390I CCNGG 5 cut(s) 1149, 1179, 1469, 1470, 1808
Bme18I GGWCC 5 cut(s) 476, 866, 962, 1130, 1175
BmeT110I CYCGRG 1 cut(s) 1468
BmgBI CACGTC 2 cut(s) 663, 844
BmgT120I GGNCC 9 cut(s) 476, 739, 866, 962, 1130, 1175, 1203, 1471, 1697
BmiI GGNNCC 5 cut(s) 655, 741, 868, 993, 1588
BmrFI CCNGG 5 cut(s) 1149, 1179, 1469, 1470, 1808
BmrI ACTGGG 1 cut(s) 477
BmsI GCATC 3 cut(s) 737, 784, 808
BmuI ACTGGG 1 cut(s) 477
BpmI CTGGAG 1 cut(s) 1863
Bpu10I CCTNAGC 1 cut(s) 1406
BpuEI CTTGAG 3 cut(s) 1563, 1749, 1894
BpuMI CCSGG 2 cut(s) 1469, 1470
BsaAI YACGTR 2 cut(s) 1547, 1803
BsaJI CCNNGG 7 cut(s) 270, 403, 649, 995, 1147, 1359, 1468
BsaWI WCCGGW 1 cut(s) 1082
BsaXI ACNNNNNCTCC 4 cut(s) 170, 200, 833, 863
Bsc4I CCNNNNNNNGG 3 cut(s) 409, 520, 971
Bse118I RCCGGY 1 cut(s) 1411
Bse1I ACTGG 2 cut(s) 472, 1325
Bse3DI GCAATG 5 cut(s) 318, 819, 924, 1137, 1287
BseAI TCCGGA 1 cut(s) 1082
BseBI CCWGG 3 cut(s) 1149, 1179, 1808
BseDI CCNNGG 7 cut(s) 270, 403, 649, 995, 1147, 1359, 1468
BseGI GGATG 6 cut(s) 249, 823, 1188, 1369, 1755, 1855
BseLI CCNNNNNNNGG 3 cut(s) 409, 520, 971
BseMI GCAATG 5 cut(s) 318, 819, 924, 1137, 1287
BseMII CTCAG 2 cut(s) 885, 1673
BseNI ACTGG 2 cut(s) 472, 1325
BseRI GAGGAG 1 cut(s) 107
BseXI GCAGC 7 cut(s) 268, 661, 796, 808, 1208, 1304, 1318
BsgI GTGCAG 3 cut(s) 50, 1455, 1650
Bsh1236I CGCG 1 cut(s) 1361
BshFI GGCC 8 cut(s) 648, 716, 740, 1204, 1411, 1440, 1473, 1698
BshNI GGYRCC 1 cut(s) 653
BsiHKAI GWGCWC 4 cut(s) 631, 849, 1310, 1773
BsiHKCI CYCGRG 1 cut(s) 1468
BsiSI CCGG 4 cut(s) 521, 1083, 1412, 1469
BslFI GGGAC 2 cut(s) 426, 852
BslI CCNNNNNNNGG 3 cut(s) 409, 520, 971
BsmAI GTCTC 1 cut(s) 1084
BsmFI GGGAC 2 cut(s) 426, 852
BsnI GGCC 8 cut(s) 648, 716, 740, 1204, 1411, 1440, 1473, 1698
BsoBI CYCGRG 1 cut(s) 1468
Bsp1286I GDGCHC 4 cut(s) 631, 849, 1310, 1773
Bsp13I TCCGGA 1 cut(s) 1082
Bsp143I GATC 7 cut(s) 138, 481, 889, 1085, 1090, 1723, 1888
Bsp19I CCATGG 1 cut(s) 649
BspACI CCGC 6 cut(s) 259, 777, 864, 1047, 1359, 1361
BspANI GGCC 8 cut(s) 648, 716, 740, 1204, 1411, 1440, 1473, 1698
BspCNI CTCAG 2 cut(s) 884, 1672
BspEI TCCGGA 1 cut(s) 1082
BspFNI CGCG 1 cut(s) 1361
BspHI TCATGA 2 cut(s) 166, 1350
BspLI GGNNCC 5 cut(s) 655, 741, 868, 993, 1588
BspMI ACCTGC 1 cut(s) 22
BspPI GGATC 3 cut(s) 1085, 1093, 1731
BspT107I GGYRCC 1 cut(s) 653
BspTI CTTAAG 1 cut(s) 1301
BsrDI GCAATG 5 cut(s) 318, 819, 924, 1137, 1287
BsrFI RCCGGY 1 cut(s) 1411
BsrI ACTGG 2 cut(s) 472, 1325
BssAI RCCGGY 1 cut(s) 1411
BssECI CCNNGG 7 cut(s) 270, 403, 649, 995, 1147, 1359, 1468
BssMI GATC 7 cut(s) 138, 481, 889, 1085, 1090, 1723, 1888
BssT1I CCWWGG 2 cut(s) 403, 649
Bst2UI CCWGG 3 cut(s) 1149, 1179, 1808
Bst4CI ACNGT 4 cut(s) 559, 1009, 1684, 1832
BstAFI CTTAAG 1 cut(s) 1301
BstAPI GCANNNNNTGC 2 cut(s) 818, 1433
BstBAI YACGTR 2 cut(s) 1547, 1803
BstC8I GCNNGC 7 cut(s) 107, 604, 700, 1018, 1144, 1413, 1438
BstDEI CTNAG 8 cut(s) 391, 871, 1230, 1280, 1406, 1659, 1729, 1932
BstDSI CCRYGG 4 cut(s) 270, 649, 995, 1359
BstEII GGTNACC 1 cut(s) 1323
BstF5I GGATG 6 cut(s) 249, 823, 1188, 1369, 1755, 1855
BstFNI CGCG 1 cut(s) 1361
BstHHI GCGC 2 cut(s) 791, 908
BstKTI GATC 7 cut(s) 141, 484, 892, 1088, 1093, 1726, 1891
BstMAI GTCTC 1 cut(s) 1084
BstMBI GATC 7 cut(s) 138, 481, 889, 1085, 1090, 1723, 1888
BstMWI GCNNNNNNNGC 8 cut(s) 235, 746, 755, 786, 818, 905, 914, 1433
BstNI CCWGG 3 cut(s) 1149, 1179, 1808
BstPI GGTNACC 1 cut(s) 1323
BstSCI CCNGG 5 cut(s) 1147, 1177, 1467, 1468, 1806
BstSFI CTRYAG 2 cut(s) 347, 1056
BstSNI TACGTA 2 cut(s) 1547, 1803
BstUI CGCG 1 cut(s) 1361
BstV1I GCAGC 7 cut(s) 268, 661, 796, 808, 1208, 1304, 1318
BstX2I RGATCY 2 cut(s) 1090, 1723
BstYI RGATCY 2 cut(s) 1090, 1723
BsuI GTATCC 4 cut(s) 457, 520, 1559, 1862
BsuRI GGCC 8 cut(s) 648, 716, 740, 1204, 1411, 1440, 1473, 1698
BtgI CCRYGG 4 cut(s) 270, 649, 995, 1359
BtrI CACGTC 2 cut(s) 663, 844
BtsCI GGATG 6 cut(s) 249, 823, 1188, 1369, 1755, 1855
BtsI GCAGTG 1 cut(s) 1572
BtsIMutI CAGTG 4 cut(s) 1005, 1023, 1572, 1837
BveI ACCTGC 1 cut(s) 22
Cac8I GCNNGC 7 cut(s) 107, 604, 700, 1018, 1144, 1413, 1438
CaiI CAGNNNCTG 2 cut(s) 677, 731
CciI TCATGA 2 cut(s) 166, 1350
CfoI GCGC 2 cut(s) 791, 908
Cfr10I RCCGGY 1 cut(s) 1411
Cfr13I GGNCC 9 cut(s) 476, 739, 866, 962, 1130, 1175, 1203, 1471, 1697
Cfr42I CCGCGG 1 cut(s) 1362
Cfr9I CCCGGG 1 cut(s) 1468
CseI GACGC 1 cut(s) 608
CsiI ACCWGGT 2 cut(s) 1177, 1806
Csp6I GTAC 4 cut(s) 1257, 1367, 1733, 1804
CspCI CAANNNNNGTGG 4 cut(s) 259, 294, 1899, 1934
CviQI GTAC 4 cut(s) 1257, 1367, 1733, 1804
DdeI CTNAG 8 cut(s) 391, 871, 1230, 1280, 1406, 1659, 1729, 1932
DpnI GATC 7 cut(s) 140, 483, 891, 1087, 1092, 1725, 1890
DpnII GATC 7 cut(s) 138, 481, 889, 1085, 1090, 1723, 1888
DraI TTTAAA 1 cut(s) 1524
EaeI YGGCCR 1 cut(s) 646
Ecl136II GAGCTC 1 cut(s) 1308
Eco105I TACGTA 2 cut(s) 1547, 1803
Eco130I CCWWGG 2 cut(s) 403, 649
Eco24I GRGCYC 1 cut(s) 1310
Eco32I GATATC 1 cut(s) 1168
Eco47I GGWCC 5 cut(s) 476, 866, 962, 1130, 1175
Eco53kI GAGCTC 1 cut(s) 1308
Eco88I CYCGRG 1 cut(s) 1468
Eco91I GGTNACC 1 cut(s) 1323
EcoICRI GAGCTC 1 cut(s) 1308
EcoO65I GGTNACC 1 cut(s) 1323
EcoRI GAATTC 3 cut(s) 148, 386, 1152
EcoRII CCWGG 3 cut(s) 1147, 1177, 1806
EcoRV GATATC 1 cut(s) 1168
EcoT14I CCWWGG 2 cut(s) 403, 649
EcoT38I GRGCYC 1 cut(s) 1310
ErhI CCWWGG 2 cut(s) 403, 649
FaqI GGGAC 2 cut(s) 426, 852
FbaI TGATCA 2 cut(s) 481, 1888
Fnu4HI GCNGC 8 cut(s) 282, 675, 778, 810, 822, 1197, 1293, 1332
FokI GGATG 6 cut(s) 256, 830, 1175, 1376, 1762, 1862
FriOI GRGCYC 1 cut(s) 1310
Fsp4HI GCNGC 8 cut(s) 282, 675, 778, 810, 822, 1197, 1293, 1332
FspBI CTAG 6 cut(s) 102, 210, 618, 666, 1193, 1905
GlaI GCGC 2 cut(s) 790, 907
GluI GCNGC 8 cut(s) 282, 675, 778, 810, 822, 1197, 1293, 1332
GsuI CTGGAG 1 cut(s) 1863
HaeIII GGCC 8 cut(s) 648, 716, 740, 1204, 1411, 1440, 1473, 1698
HapII CCGG 4 cut(s) 521, 1083, 1412, 1469
HgaI GACGC 1 cut(s) 608
HhaI GCGC 2 cut(s) 791, 908
Hin6I GCGC 2 cut(s) 789, 906
HinP1I GCGC 2 cut(s) 789, 906
HincII GTYRAC 3 cut(s) 841, 1447, 1789
HindII GTYRAC 3 cut(s) 841, 1447, 1789
HindIII AAGCTT 2 cut(s) 572, 700
HinfI GANTC 3 cut(s) 1000, 1657, 1759
HpaI GTTAAC 2 cut(s) 1447, 1789
HpaII CCGG 4 cut(s) 521, 1083, 1412, 1469
HphI GGTGA 5 cut(s) 449, 476, 1268, 1317, 1546
Hpy166II GTNNAC 7 cut(s) 841, 1216, 1447, 1570, 1656, 1714, 1789
Hpy188I TCNGA 3 cut(s) 894, 1090, 1888
Hpy188III TCNNGA 8 cut(s) 167, 383, 965, 1083, 1125, 1351, 1687, 1842
Hpy8I GTNNAC 7 cut(s) 841, 1216, 1447, 1570, 1656, 1714, 1789
HpyAV CCTTC 8 cut(s) 320, 434, 497, 498, 615, 686, 1461, 1626
HpyCH4III ACNGT 4 cut(s) 559, 1009, 1684, 1832
HpyCH4IV ACGT 7 cut(s) 662, 801, 843, 1259, 1546, 1692, 1802
HpyF10VI GCNNNNNNNGC 8 cut(s) 235, 746, 755, 786, 818, 905, 914, 1433
HpyF3I CTNAG 8 cut(s) 391, 871, 1230, 1280, 1406, 1659, 1729, 1932
HpySE526I ACGT 7 cut(s) 662, 801, 843, 1259, 1546, 1692, 1802
HspAI GCGC 2 cut(s) 789, 906
Kpn2I TCCGGA 1 cut(s) 1082
KroI GCCGGC 1 cut(s) 1411
KroNI GCCGGC 1 cut(s) 1413
Ksp22I TGATCA 2 cut(s) 481, 1888
KspAI GTTAAC 2 cut(s) 1447, 1789
KspI CCGCGG 1 cut(s) 1362
Kzo9I GATC 7 cut(s) 138, 481, 889, 1085, 1090, 1723, 1888
LmnI GCTCC 7 cut(s) 94, 634, 914, 997, 1013, 1105, 1586
Lsp1109I GCAGC 7 cut(s) 268, 661, 796, 808, 1208, 1304, 1318
LweI GCATC 3 cut(s) 737, 784, 808
MabI ACCWGGT 2 cut(s) 1177, 1806
MaeI CTAG 6 cut(s) 102, 210, 618, 666, 1193, 1905
MaeII ACGT 7 cut(s) 662, 801, 843, 1259, 1546, 1692, 1802
MaeIII GTNAC 4 cut(s) 1323, 1643, 1860, 1900
MalI GATC 7 cut(s) 140, 483, 891, 1087, 1092, 1725, 1890
MboI GATC 7 cut(s) 138, 481, 889, 1085, 1090, 1723, 1888
MboII GAAGA 4 cut(s) 16, 35, 182, 1607
MfeI CAATTG 1 cut(s) 1461
MflI RGATCY 2 cut(s) 1090, 1723
MhlI GDGCHC 4 cut(s) 631, 849, 1310, 1773
MlsI TGGCCA 1 cut(s) 648
MluNI TGGCCA 1 cut(s) 648
MlyI GAGTC 1 cut(s) 1651
MmeI TCCRAC 2 cut(s) 991, 1564
Mox20I TGGCCA 1 cut(s) 648
MroI TCCGGA 1 cut(s) 1082
MroNI GCCGGC 1 cut(s) 1411
MscI TGGCCA 1 cut(s) 648
MseI TTAA 8 cut(s) 53, 1037, 1242, 1302, 1338, 1446, 1523, 1788
MslI CAYNNNNRTG 1 cut(s) 1281
Msp20I TGGCCA 1 cut(s) 648
MspA1I CMGCKG 6 cut(s) 229, 674, 942, 950, 1331, 1361
MspCI CTTAAG 1 cut(s) 1301
MspI CCGG 4 cut(s) 521, 1083, 1412, 1469
MspR9I CCNGG 5 cut(s) 1149, 1179, 1469, 1470, 1808
MssI GTTTAAAC 1 cut(s) 1524
MunI CAATTG 1 cut(s) 1461
MvaI CCWGG 3 cut(s) 1149, 1179, 1808
MvnI CGCG 1 cut(s) 1361
MwoI GCNNNNNNNGC 8 cut(s) 235, 746, 755, 786, 818, 905, 914, 1433
NaeI GCCGGC 1 cut(s) 1413
NciI CCSGG 2 cut(s) 1469, 1470
NcoI CCATGG 1 cut(s) 649
NdeII GATC 7 cut(s) 138, 481, 889, 1085, 1090, 1723, 1888
NgoMIV GCCGGC 1 cut(s) 1411
NlaIV GGNNCC 5 cut(s) 655, 741, 868, 993, 1588
NmuCI GTSAC 3 cut(s) 1323, 1643, 1860
OliI CACNNNNGTG 1 cut(s) 1281
PagI TCATGA 2 cut(s) 166, 1350
PaqCI CACCTGC 1 cut(s) 22
PdiI GCCGGC 1 cut(s) 1413
PfeI GAWTC 2 cut(s) 1000, 1759
PflFI GACNNNGTC 1 cut(s) 1660
PflMI CCANNNNNTGG 1 cut(s) 409
PkrI GCNGC 8 cut(s) 283, 676, 779, 811, 823, 1198, 1294, 1333
PleI GAGTC 1 cut(s) 1651
PmeI GTTTAAAC 1 cut(s) 1524
PpsI GAGTC 1 cut(s) 1651
Ppu21I YACGTR 2 cut(s) 1547, 1803
PsiI TTATAA 1 cut(s) 765
Psp124BI GAGCTC 1 cut(s) 1310
Psp1406I AACGTT 1 cut(s) 1692
Psp6I CCWGG 3 cut(s) 1147, 1177, 1806
PspEI GGTNACC 1 cut(s) 1323
PspGI CCWGG 3 cut(s) 1147, 1177, 1806
PspN4I GGNNCC 5 cut(s) 655, 741, 868, 993, 1588
PspPI GGNCC 9 cut(s) 476, 739, 866, 962, 1130, 1175, 1203, 1471, 1697
PstNI CAGNNNCTG 2 cut(s) 677, 731
PsuI RGATCY 2 cut(s) 1090, 1723
PsyI GACNNNGTC 1 cut(s) 1660
PvuII CAGCTG 5 cut(s) 229, 674, 942, 950, 1331
RsaI GTAC 4 cut(s) 1258, 1368, 1734, 1805
RsaNI GTAC 4 cut(s) 1257, 1367, 1733, 1804
RseI CAYNNNNRTG 1 cut(s) 1281
SacI GAGCTC 1 cut(s) 1310
SacII CCGCGG 1 cut(s) 1362
SaqAI TTAA 8 cut(s) 53, 1037, 1242, 1302, 1338, 1446, 1523, 1788
SatI GCNGC 8 cut(s) 282, 675, 778, 810, 822, 1197, 1293, 1332
Sau3AI GATC 7 cut(s) 138, 481, 889, 1085, 1090, 1723, 1888
Sau96I GGNCC 9 cut(s) 476, 739, 866, 962, 1130, 1175, 1203, 1471, 1697
SchI GAGTC 1 cut(s) 1651
ScrFI CCNGG 5 cut(s) 1149, 1179, 1469, 1470, 1808
SduI GDGCHC 4 cut(s) 631, 849, 1310, 1773
SexAI ACCWGGT 2 cut(s) 1177, 1806
SfaNI GCATC 3 cut(s) 737, 784, 808
SfcI CTRYAG 2 cut(s) 347, 1056
Sfr303I CCGCGG 1 cut(s) 1362
SgrBI CCGCGG 1 cut(s) 1362
SinI GGWCC 5 cut(s) 476, 866, 962, 1130, 1175
SmaI CCCGGG 1 cut(s) 1470
SmiMI CAYNNNNRTG 1 cut(s) 1281
SmlI CTYRAG 4 cut(s) 1301, 1578, 1764, 1873
SmoI CTYRAG 4 cut(s) 1301, 1578, 1764, 1873
SnaBI TACGTA 2 cut(s) 1547, 1803
SpeI ACTAGT 1 cut(s) 209
SsiI CCGC 6 cut(s) 259, 777, 864, 1047, 1359, 1361
SspI AATATT 1 cut(s) 1035
SspMI CTAG 6 cut(s) 102, 210, 618, 666, 1193, 1905
SstI GAGCTC 1 cut(s) 1310
StyD4I CCNGG 5 cut(s) 1147, 1177, 1467, 1468, 1806
StyI CCWWGG 2 cut(s) 403, 649
TaaI ACNGT 4 cut(s) 559, 1009, 1684, 1832
TaiI ACGT 7 cut(s) 665, 804, 846, 1262, 1549, 1695, 1805
TaqI TCGA 1 cut(s) 1395
TaqII GACCGA 1 cut(s) 1044
TatI WGTACW 1 cut(s) 1366
TauI GCSGC 1 cut(s) 780
TfiI GAWTC 2 cut(s) 1000, 1759
Tru1I TTAA 8 cut(s) 53, 1037, 1242, 1302, 1338, 1446, 1523, 1788
Tru9I TTAA 8 cut(s) 53, 1037, 1242, 1302, 1338, 1446, 1523, 1788
TscAI CASTG 4 cut(s) 1012, 1030, 1572, 1837
TseFI GTSAC 3 cut(s) 1323, 1643, 1860
TseI GCWGC 7 cut(s) 281, 674, 809, 821, 1196, 1292, 1331
Tsp45I GTSAC 3 cut(s) 1323, 1643, 1860
TspGWI ACGGA 2 cut(s) 567, 984
TspMI CCCGGG 1 cut(s) 1468
TspRI CASTG 4 cut(s) 1012, 1030, 1572, 1837
Tth111I GACNNNGTC 1 cut(s) 1660
Van91I CCANNNNNTGG 1 cut(s) 409
Vha464I CTTAAG 1 cut(s) 1301
VpaK11BI GGWCC 5 cut(s) 476, 866, 962, 1130, 1175
XapI RAATTY 6 cut(s) 148, 386, 589, 682, 930, 1152
XcmI CCANNNNNNNNNTGG 1 cut(s) 647
XmaI CCCGGG 1 cut(s) 1468
XspI CTAG 6 cut(s) 102, 210, 618, 666, 1193, 1905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.