FvH4_7g08400

Subtilisin-like serine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000302
Physical Location & Seq
Reverse (-)
8292748 .. 8294883
2136 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g08400.t1

Sequence Viewer

Length: 2043 bp
ATGCTTTACTCATACCGTAATGTGGCCACCGGCTTTGCAGCGAAACTGACTCCAGAGGAAATTAAAGCAATGGAAAAGAAAGATGGGTTCGTGCATGCTCATCCAGAGAGGTTTCTGCCTTTGCACACAACTCATAGTCCAAACTTCCTGGGGCTGCAACAAGTGTTTGGATTTTGGAAAAAGACCAATTATGGTGAAGGAATGATCATAGGAGTTTTGGACACTGGGATATCTCCAGGTCATCCCTCATTTAGTGATGAAGGAGTGCCACCTCCCCCAGAGAAATGGAAAGGCAAATGCGAGTTCAAGTTCAATGGGAGAACAGTGTGCAACAACAAGCTTATTGGTGCAAGAAATTTTCAAGGTGGAAAAGAAACAAAAGAAGTCTCTCCATTTGGTGAAGAAGGCCATGGCACACTCACTTCTAGCATTGCTGCTGGAAACTTTGTGGAAGGCGCCAGCGTGTTTGGAATGGCAAATGGCACAGCATCTGGCATTGCACCTTATGCTCACTTGGCAATCTACAAGGTCTGCACCGAGGCAGGTTGTGCCGAAAGTGACATACTAGCAGCTATTGACATTGCTGTTGAGGACGGCGTAGATGTGCTCTCACTTTCCATAGGTTTTGCTTCCTCGGTTCCTTTCCATGAAGATATGGTTGCTATCGGCGCGTTTGGAGCATTTCGAAAGGGAATTTTCGTCAGCTGTTCAGCAGGAAACTCTGGCCCTTCCTATAGTTCCATGTCAAACGAGGCACCTTGGATTCTCACTGTTGGAGCAAGCACCATTGACAGGAAAATAAGCTCAATAGCACAACTTGGAGATTCTCCAAAATACTTTTACTCTGGTGAATCATTATTCGATCCTAAAAATTTTAGCACTTCACCATTGCTGCCTCTTGTTTATCCAGGTGCAATTCACAATAACAAAACTGAGTTCTGTGCCTTGGGATCTCTTGTAAATGTTGAAGGGAAAGTAGTGTTGTGTGAGAGAGGTGGAGGAGTGGGAGGAACTGCCAAAGGGGAAGAAGTGAAAAGAGCAGGAGGTGCTGCCATGATTCTAGTGAACGAAGTAACTGATGGCTATAGCACATTAGCTAATGCTTATGTACTTCCAGCAACAAATGTTGGTTTTCAAGCAGGTTTGAGTATCAAAACCTATATAAACTCAACAACTACACCTATAGCCACAGTTTATTCCCCACGCACTGTAATCGGAGATGCACTTGCTCCAATGGTTGCTTCTTTCTCATCAAGAGGACCGAGTATAGCTAGCCCCGGAATTCTAAAACCTGACATAATTGGTCCTGGTGTTAACATCCTTGCTGCATGGCCTGTTTCGGTGGATAATGTCACAAAATTTGACATTCTTTCAGGTACTTCGGTGTCATGCCCACACCTAAGTGGCATTGCAGCCTTGATCAAGAGTTCACATCCGGACTGGTCACCTGCTGCCATCAAATCCGCCATTATGACAACCGCTGATCTCTTAAACCTCAAAGGCACACCAATTGTTGATAGATCAAATACCCCAGCAGACATCTATAGCACTGGCTCAGGCCATGTCAACCCTTCAAAAGCCAATGACCCGGGGCTCATCTATGATATAAGACCAGACGATTACATTCCTTACTTGTGTGGTCTGAACTACACAAACAATCAAATAGAGATGATCACTCAACAAAGAGTGGACTGCTCTGAAGTAGGAGTCATCGCTGAAGCGCAGCTAAACTATCCTTCATTTTCTATCACAATAGGGTCCTATCAAACTCAGAATTACACAAGAACGGTGAAAAATGTTGGCCCAGCTTATTCAACCTACAAATCAGAGATTGGGGAACCCCAGAAATGGGGTGTGATTGTGAACGTGGGTGTGACTCCTTCGGTTCTTACATTCACAGAGGTCAACCAGACCATTACATATAATGTGGAGTTCTCCACAAAACTAGTTGGGCAAGATGGTTCATTTTTACAGGGACATTTGAGATGGGTTTCTGACAACCATTCTGTATACAGCCCCATAGCTGTCATCTTTCACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

681

Amino Acids

72.2

Weight (kDa)

5.78

Isoelectric Point (pI)

30.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 1 - 42 2.8e-06 Peptidase inhibitor I9
Peptidase_S8 PF00082 65 - 498 3.5e-45 Subtilase family
PA PF02225 299 - 383 1e-09 PA domain
fn3_6 PF17766 575 - 676 6.1e-23 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1456
Acc36I ACCTGC 3 cut(s) 533, 1130, 1456
AccB1I GGYRCC 2 cut(s) 455, 754
AccI GTMKAC 1 cut(s) 2010
AccII CGCG 1 cut(s) 671
AccIII TCCGGA 1 cut(s) 1435
AciI CCGC 2 cut(s) 1464, 1479
AclWI GGATC 2 cut(s) 857, 958
AcoI YGGCCR 1 cut(s) 24
AcsI RAATTY 5 cut(s) 355, 693, 871, 1281, 1358
AcuI CTGAAG 2 cut(s) 1719, 1737
AcyI GRCGYC 1 cut(s) 456
AfaI GTAC 2 cut(s) 1110, 1378
AfiI CCNNNNNNNGG 4 cut(s) 22, 792, 1848, 1849
AgsI TTSAA 7 cut(s) 307, 313, 362, 968, 1136, 1575, 1815
AhlI ACTAGT 1 cut(s) 1945
AjnI CCWGG 4 cut(s) 147, 235, 907, 1306
AjuI GAANNNNNNNTTGG 2 cut(s) 1225, 1257
AleI CACNNNNGTG 1 cut(s) 1401
AluBI AGCT 9 cut(s) 340, 572, 705, 804, 1097, 1271, 1726, 1808, 2024
AluI AGCT 9 cut(s) 340, 572, 705, 804, 1097, 1271, 1726, 1808, 2024
Alw21I GWGCWC 1 cut(s) 609
Alw26I GTCTC 1 cut(s) 391
AlwI GGATC 2 cut(s) 857, 958
AlwNI CAGNNNCTG 1 cut(s) 491
Ama87I CYCGRG 1 cut(s) 1588
Aor13HI TCCGGA 1 cut(s) 1435
AoxI GGCC 6 cut(s) 24, 406, 724, 1331, 1558, 1801
ApoI RAATTY 5 cut(s) 355, 693, 871, 1281, 1358
AspLEI GCGC 3 cut(s) 458, 671, 1723
AspS9I GGNCC 5 cut(s) 725, 1259, 1304, 1758, 1802
AsuC2I CCSGG 3 cut(s) 1278, 1589, 1590
AsuHPI GGTGA 6 cut(s) 206, 410, 860, 876, 1437, 1801
AsuII TTCGAA 1 cut(s) 685
AsuNHI GCTAGC 1 cut(s) 1271
AvaI CYCGRG 1 cut(s) 1588
AvaII GGWCC 3 cut(s) 1259, 1304, 1758
BaeI ACNNNNGTAYC 2 cut(s) 1368, 1401
BalI TGGCCA 1 cut(s) 26
BanI GGYRCC 2 cut(s) 455, 754
BanII GRGCYC 1 cut(s) 1596
Bbv12I GWGCWC 1 cut(s) 609
BccI CCATC 5 cut(s) 77, 1073, 1463, 1952, 1980
BceAI ACGGC 1 cut(s) 610
BcgI CGANNNNNNTGC 2 cut(s) 1195, 1229
BciT130I CCWGG 4 cut(s) 149, 237, 909, 1308
BclI TGATCA 3 cut(s) 204, 1419, 1671
BcnI CCSGG 3 cut(s) 1278, 1589, 1590
BcoDI GTCTC 1 cut(s) 391
BcuI ACTAGT 1 cut(s) 1945
BfaI CTAG 5 cut(s) 426, 566, 1061, 1272, 1946
BfmI CTRYAG 4 cut(s) 733, 1084, 1182, 1543
BfoI RGCGCY 1 cut(s) 459
BfuAI ACCTGC 3 cut(s) 533, 1130, 1456
Bme1390I CCNGG 7 cut(s) 149, 237, 909, 1278, 1308, 1589, 1590
Bme18I GGWCC 3 cut(s) 1259, 1304, 1758
BmeT110I CYCGRG 1 cut(s) 1588
BmgT120I GGNCC 5 cut(s) 725, 1259, 1304, 1758, 1802
BmiI GGNNCC 5 cut(s) 457, 639, 756, 1759, 1839
BmrFI CCNGG 7 cut(s) 149, 237, 909, 1278, 1308, 1589, 1590
BmrI ACTGGG 1 cut(s) 234
BmsI GCATC 2 cut(s) 497, 1210
BmtI GCTAGC 1 cut(s) 1275
BmuI ACTGGG 1 cut(s) 234
BpmI CTGGAG 2 cut(s) 36, 219
Bpu10I CCTNAGC 1 cut(s) 1555
Bpu14I TTCGAA 1 cut(s) 685
BpuMI CCSGG 3 cut(s) 1278, 1589, 1590
BsaHI GRCGYC 1 cut(s) 456
BsaJI CCNNGG 9 cut(s) 148, 409, 537, 633, 758, 945, 1276, 1588, 1589
BsaWI WCCGGW 1 cut(s) 1435
Bsc4I CCNNNNNNNGG 4 cut(s) 22, 792, 1848, 1849
Bse118I RCCGGY 1 cut(s) 29
Bse1I ACTGG 3 cut(s) 229, 1445, 1555
Bse3DI GCAATG 6 cut(s) 75, 429, 495, 579, 887, 1407
BseAI TCCGGA 1 cut(s) 1435
BseBI CCWGG 4 cut(s) 149, 237, 909, 1308
BseDI CCNNGG 9 cut(s) 148, 409, 537, 633, 758, 945, 1276, 1588, 1589
BseGI GGATG 4 cut(s) 100, 241, 1317, 1432
BseLI CCNNNNNNNGG 4 cut(s) 22, 792, 1848, 1849
BseMI GCAATG 6 cut(s) 75, 429, 495, 579, 887, 1407
BseMII CTCAG 3 cut(s) 924, 1569, 1784
BseNI ACTGG 3 cut(s) 229, 1445, 1555
BseRI GAGGAG 1 cut(s) 1014
BseYI CCCAGC 2 cut(s) 1531, 1804
BsgI GTGCAG 1 cut(s) 517
Bsh1236I CGCG 1 cut(s) 671
BshFI GGCC 6 cut(s) 26, 408, 726, 1333, 1560, 1803
BshNI GGYRCC 2 cut(s) 455, 754
BsiHKAI GWGCWC 1 cut(s) 609
BsiHKCI CYCGRG 1 cut(s) 1588
BsiSI CCGG 4 cut(s) 30, 1278, 1436, 1589
BslFI GGGAC 1 cut(s) 1989
BslI CCNNNNNNNGG 4 cut(s) 22, 792, 1848, 1849
BsmAI GTCTC 1 cut(s) 391
BsmFI GGGAC 1 cut(s) 1989
BsnI GGCC 6 cut(s) 26, 408, 726, 1333, 1560, 1803
BsoBI CYCGRG 1 cut(s) 1588
Bsp119I TTCGAA 1 cut(s) 685
Bsp1286I GDGCHC 2 cut(s) 609, 1596
Bsp13I TCCGGA 1 cut(s) 1435
Bsp143I GATC 7 cut(s) 204, 862, 950, 1419, 1483, 1520, 1671
Bsp19I CCATGG 1 cut(s) 409
BspACI CCGC 2 cut(s) 1464, 1479
BspANI GGCC 6 cut(s) 26, 408, 726, 1333, 1560, 1803
BspCNI CTCAG 3 cut(s) 925, 1568, 1783
BspEI TCCGGA 1 cut(s) 1435
BspFNI CGCG 1 cut(s) 671
BspLI GGNNCC 5 cut(s) 457, 639, 756, 1759, 1839
BspMI ACCTGC 3 cut(s) 533, 1130, 1456
BspOI GCTAGC 1 cut(s) 1275
BspPI GGATC 2 cut(s) 857, 958
BspT104I TTCGAA 1 cut(s) 685
BspT107I GGYRCC 2 cut(s) 455, 754
BsrDI GCAATG 6 cut(s) 75, 429, 495, 579, 887, 1407
BsrFI RCCGGY 1 cut(s) 29
BsrI ACTGG 3 cut(s) 229, 1445, 1555
BssAI RCCGGY 1 cut(s) 29
BssECI CCNNGG 9 cut(s) 148, 409, 537, 633, 758, 945, 1276, 1588, 1589
BssMI GATC 7 cut(s) 204, 862, 950, 1419, 1483, 1520, 1671
BssNAI GTATAC 1 cut(s) 2011
BssNI GRCGYC 1 cut(s) 456
BssT1I CCWWGG 3 cut(s) 409, 758, 945
Bst1107I GTATAC 1 cut(s) 2011
Bst2UI CCWGG 4 cut(s) 149, 237, 909, 1308
Bst4CI ACNGT 6 cut(s) 17, 325, 772, 1192, 1210, 1789
BstACI GRCGYC 1 cut(s) 456
BstAPI GCANNNNNTGC 3 cut(s) 506, 548, 1046
BstBI TTCGAA 1 cut(s) 685
BstC8I GCNNGC 4 cut(s) 96, 460, 781, 1273
BstDEI CTNAG 4 cut(s) 933, 1400, 1555, 1770
BstDSI CCRYGG 1 cut(s) 409
BstEII GGTNACC 1 cut(s) 1443
BstF5I GGATG 4 cut(s) 100, 241, 1317, 1432
BstFNI CGCG 1 cut(s) 671
BstH2I RGCGCY 1 cut(s) 459
BstHHI GCGC 3 cut(s) 458, 671, 1723
BstKTI GATC 7 cut(s) 207, 865, 953, 1422, 1486, 1523, 1674
BstMAI GTCTC 1 cut(s) 391
BstMBI GATC 7 cut(s) 204, 862, 950, 1419, 1483, 1520, 1671
BstMWI GCNNNNNNNGC 6 cut(s) 506, 515, 548, 668, 677, 1046
BstNI CCWGG 4 cut(s) 149, 237, 909, 1308
BstNSI RCATGY 1 cut(s) 98
BstPI GGTNACC 1 cut(s) 1443
BstSCI CCNGG 7 cut(s) 147, 235, 907, 1276, 1306, 1587, 1588
BstSFI CTRYAG 4 cut(s) 733, 1084, 1182, 1543
BstUI CGCG 1 cut(s) 671
BstX2I RGATCY 1 cut(s) 950
BstXI CCANNNNNNTGG 1 cut(s) 285
BstYI RGATCY 1 cut(s) 950
BstZ17I GTATAC 1 cut(s) 2011
BsuRI GGCC 6 cut(s) 26, 408, 726, 1333, 1560, 1803
BtgI CCRYGG 1 cut(s) 409
BtgZI GCGATG 1 cut(s) 1696
BtsCI GGATG 4 cut(s) 100, 241, 1317, 1432
BtsIMutI CAGTG 5 cut(s) 222, 330, 768, 1206, 1548
BveI ACCTGC 3 cut(s) 533, 1130, 1456
Cac8I GCNNGC 4 cut(s) 96, 460, 781, 1273
CaiI CAGNNNCTG 1 cut(s) 491
CfoI GCGC 3 cut(s) 458, 671, 1723
Cfr10I RCCGGY 1 cut(s) 29
Cfr13I GGNCC 5 cut(s) 725, 1259, 1304, 1758, 1802
Cfr9I CCCGGG 1 cut(s) 1588
Csp6I GTAC 2 cut(s) 1109, 1377
CspCI CAANNNNNGTGG 2 cut(s) 16, 51
CviAII CATG 8 cut(s) 95, 410, 647, 742, 1054, 1329, 1389, 1562
CviQI GTAC 2 cut(s) 1109, 1377
DdeI CTNAG 4 cut(s) 933, 1400, 1555, 1770
DinI GGCGCC 1 cut(s) 457
DpnI GATC 7 cut(s) 206, 864, 952, 1421, 1485, 1522, 1673
DpnII GATC 7 cut(s) 204, 862, 950, 1419, 1483, 1520, 1671
EaeI YGGCCR 1 cut(s) 24
EciI GGCGGA 1 cut(s) 1453
Eco130I CCWWGG 3 cut(s) 409, 758, 945
Eco24I GRGCYC 1 cut(s) 1596
Eco32I GATATC 1 cut(s) 231
Eco47I GGWCC 3 cut(s) 1259, 1304, 1758
Eco57I CTGAAG 2 cut(s) 1719, 1737
Eco88I CYCGRG 1 cut(s) 1588
Eco91I GGTNACC 1 cut(s) 1443
EcoO109I RGGNCCY 1 cut(s) 1758
EcoO65I GGTNACC 1 cut(s) 1443
EcoRI GAATTC 1 cut(s) 1281
EcoRII CCWGG 4 cut(s) 147, 235, 907, 1306
EcoRV GATATC 1 cut(s) 231
EcoT14I CCWWGG 3 cut(s) 409, 758, 945
EcoT38I GRGCYC 1 cut(s) 1596
EgeI GGCGCC 1 cut(s) 457
EheI GGCGCC 1 cut(s) 457
ErhI CCWWGG 3 cut(s) 409, 758, 945
FaeI CATG 8 cut(s) 98, 413, 650, 745, 1057, 1332, 1392, 1565
FaqI GGGAC 1 cut(s) 1989
FatI CATG 8 cut(s) 94, 409, 646, 741, 1053, 1328, 1388, 1561
FbaI TGATCA 3 cut(s) 204, 1419, 1671
FblI GTMKAC 1 cut(s) 2010
FokI GGATG 4 cut(s) 87, 228, 1304, 1419
FriOI GRGCYC 1 cut(s) 1596
FspBI CTAG 5 cut(s) 426, 566, 1061, 1272, 1946
GlaI GCGC 3 cut(s) 457, 670, 1722
GsaI CCCAGC 2 cut(s) 1535, 1808
GsuI CTGGAG 2 cut(s) 36, 219
HaeII RGCGCY 1 cut(s) 459
HaeIII GGCC 6 cut(s) 26, 408, 726, 1333, 1560, 1803
HapII CCGG 4 cut(s) 30, 1278, 1436, 1589
HhaI GCGC 3 cut(s) 458, 671, 1723
Hin1I GRCGYC 1 cut(s) 456
Hin1II CATG 8 cut(s) 98, 413, 650, 745, 1057, 1332, 1392, 1565
Hin6I GCGC 3 cut(s) 456, 669, 1721
HinP1I GCGC 3 cut(s) 456, 669, 1721
HincII GTYRAC 3 cut(s) 1315, 1567, 1906
HindII GTYRAC 3 cut(s) 1315, 1567, 1906
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 7 cut(s) 49, 763, 824, 851, 1057, 1707, 1876
HpaI GTTAAC 1 cut(s) 1315
HpaII CCGG 4 cut(s) 30, 1278, 1436, 1589
HphI GGTGA 6 cut(s) 206, 410, 860, 876, 1437, 1801
Hpy166II GTNNAC 8 cut(s) 1066, 1315, 1430, 1567, 1690, 1864, 1906, 2011
Hpy188I TCNGA 6 cut(s) 1217, 1644, 1699, 1773, 1828, 1996
Hpy188III TCNNGA 6 cut(s) 53, 104, 1254, 1423, 1436, 2040
Hpy8I GTNNAC 8 cut(s) 1066, 1315, 1430, 1567, 1690, 1864, 1906, 2011
HpyAV CCTTC 9 cut(s) 191, 254, 398, 446, 738, 962, 1581, 1746, 1890
HpyCH4III ACNGT 6 cut(s) 17, 325, 772, 1192, 1210, 1789
HpyCH4IV ACGT 1 cut(s) 1866
HpyF10VI GCNNNNNNNGC 6 cut(s) 506, 515, 548, 668, 677, 1046
HpyF3I CTNAG 4 cut(s) 933, 1400, 1555, 1770
HpySE526I ACGT 1 cut(s) 1866
Hsp92I GRCGYC 1 cut(s) 456
Hsp92II CATG 8 cut(s) 98, 413, 650, 745, 1057, 1332, 1392, 1565
HspAI GCGC 3 cut(s) 456, 669, 1721
KasI GGCGCC 1 cut(s) 455
Kpn2I TCCGGA 1 cut(s) 1435
Ksp22I TGATCA 3 cut(s) 204, 1419, 1671
KspAI GTTAAC 1 cut(s) 1315
Kzo9I GATC 7 cut(s) 204, 862, 950, 1419, 1483, 1520, 1671
LmnI GCTCC 3 cut(s) 677, 776, 1234
LweI GCATC 2 cut(s) 497, 1210
MaeI CTAG 5 cut(s) 426, 566, 1061, 1272, 1946
MaeII ACGT 1 cut(s) 1866
MaeIII GTNAC 5 cut(s) 557, 1072, 1351, 1443, 1873
MalI GATC 7 cut(s) 206, 864, 952, 1421, 1485, 1522, 1673
MboI GATC 7 cut(s) 204, 862, 950, 1419, 1483, 1520, 1671
MboII GAAGA 3 cut(s) 413, 662, 1037
MfeI CAATTG 1 cut(s) 1509
MflI RGATCY 1 cut(s) 950
MhlI GDGCHC 2 cut(s) 609, 1596
MlsI TGGCCA 1 cut(s) 26
MluNI TGGCCA 1 cut(s) 26
Mly113I GGCGCC 1 cut(s) 456
MlyI GAGTC 3 cut(s) 43, 1716, 1870
MmeI TCCRAC 1 cut(s) 754
Mox20I TGGCCA 1 cut(s) 26
MroI TCCGGA 1 cut(s) 1435
MscI TGGCCA 1 cut(s) 26
MseI TTAA 3 cut(s) 63, 1314, 1490
MslI CAYNNNNRTG 1 cut(s) 1401
Msp20I TGGCCA 1 cut(s) 26
MspA1I CMGCKG 2 cut(s) 705, 1481
MspI CCGG 4 cut(s) 30, 1278, 1436, 1589
MspR9I CCNGG 7 cut(s) 149, 237, 909, 1278, 1308, 1589, 1590
MunI CAATTG 1 cut(s) 1509
MvaI CCWGG 4 cut(s) 149, 237, 909, 1308
MvnI CGCG 1 cut(s) 671
MwoI GCNNNNNNNGC 6 cut(s) 506, 515, 548, 668, 677, 1046
NarI GGCGCC 1 cut(s) 456
NciI CCSGG 3 cut(s) 1278, 1589, 1590
NcoI CCATGG 1 cut(s) 409
NdeII GATC 7 cut(s) 204, 862, 950, 1419, 1483, 1520, 1671
NheI GCTAGC 1 cut(s) 1271
NlaIII CATG 8 cut(s) 98, 413, 650, 745, 1057, 1332, 1392, 1565
NlaIV GGNNCC 5 cut(s) 457, 639, 756, 1759, 1839
NmuCI GTSAC 4 cut(s) 557, 1351, 1443, 1873
NspI RCATGY 1 cut(s) 98
NspV TTCGAA 1 cut(s) 685
OliI CACNNNNGTG 1 cut(s) 1401
PaeI GCATGC 1 cut(s) 98
PaqCI CACCTGC 1 cut(s) 1456
PfeI GAWTC 4 cut(s) 763, 824, 851, 1057
PleI GAGTC 3 cut(s) 43, 1715, 1870
PluTI GGCGCC 1 cut(s) 459
PpsI GAGTC 3 cut(s) 43, 1715, 1870
PpuMI RGGWCCY 1 cut(s) 1758
Psp5II RGGWCCY 1 cut(s) 1758
Psp6I CCWGG 4 cut(s) 147, 235, 907, 1306
PspEI GGTNACC 1 cut(s) 1443
PspFI CCCAGC 2 cut(s) 1531, 1804
PspGI CCWGG 4 cut(s) 147, 235, 907, 1306
PspN4I GGNNCC 5 cut(s) 457, 639, 756, 1759, 1839
PspPI GGNCC 5 cut(s) 725, 1259, 1304, 1758, 1802
PspPPI RGGWCCY 1 cut(s) 1758
PstNI CAGNNNCTG 1 cut(s) 491
PsuI RGATCY 1 cut(s) 950
PvuII CAGCTG 1 cut(s) 705
RsaI GTAC 2 cut(s) 1110, 1378
RsaNI GTAC 2 cut(s) 1109, 1377
RseI CAYNNNNRTG 1 cut(s) 1401
SaqAI TTAA 3 cut(s) 63, 1314, 1490
Sau3AI GATC 7 cut(s) 204, 862, 950, 1419, 1483, 1520, 1671
Sau96I GGNCC 5 cut(s) 725, 1259, 1304, 1758, 1802
SchI GAGTC 3 cut(s) 43, 1716, 1870
ScrFI CCNGG 7 cut(s) 149, 237, 909, 1278, 1308, 1589, 1590
SduI GDGCHC 2 cut(s) 609, 1596
SfaNI GCATC 2 cut(s) 497, 1210
SfcI CTRYAG 4 cut(s) 733, 1084, 1182, 1543
SfoI GGCGCC 1 cut(s) 457
SfuI TTCGAA 1 cut(s) 685
SinI GGWCC 3 cut(s) 1259, 1304, 1758
SmaI CCCGGG 1 cut(s) 1590
SmiMI CAYNNNNRTG 1 cut(s) 1401
SpeI ACTAGT 1 cut(s) 1945
SphI GCATGC 1 cut(s) 98
SsiI CCGC 2 cut(s) 1464, 1479
SspDI GGCGCC 1 cut(s) 455
SspMI CTAG 5 cut(s) 426, 566, 1061, 1272, 1946
StyD4I CCNGG 7 cut(s) 147, 235, 907, 1276, 1306, 1587, 1588
StyI CCWWGG 3 cut(s) 409, 758, 945
TaaI ACNGT 6 cut(s) 17, 325, 772, 1192, 1210, 1789
TaiI ACGT 1 cut(s) 1869
TaqI TCGA 2 cut(s) 685, 861
TaqII GACCGA 1 cut(s) 1276
TatI WGTACW 1 cut(s) 1108
TfiI GAWTC 4 cut(s) 763, 824, 851, 1057
Tru1I TTAA 3 cut(s) 63, 1314, 1490
Tru9I TTAA 3 cut(s) 63, 1314, 1490
TscAI CASTG 5 cut(s) 229, 330, 775, 1213, 1555
TseFI GTSAC 4 cut(s) 557, 1351, 1443, 1873
Tsp45I GTSAC 4 cut(s) 557, 1351, 1443, 1873
TspDTI ATGAA 4 cut(s) 273, 663, 1728, 1953
TspMI CCCGGG 1 cut(s) 1588
TspRI CASTG 5 cut(s) 229, 330, 775, 1213, 1555
VpaK11BI GGWCC 3 cut(s) 1259, 1304, 1758
XapI RAATTY 5 cut(s) 355, 693, 871, 1281, 1358
XceI RCATGY 1 cut(s) 98
XmaI CCCGGG 1 cut(s) 1588
XmiI GTMKAC 1 cut(s) 2010
XspI CTAG 5 cut(s) 426, 566, 1061, 1272, 1946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.