Rmu_sc0006398.1_g000001

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006398.1
OG0000302
Physical Location & Seq
Reverse (-)
935 .. 2176
1242 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006398.1_g000001.1.cds

Sequence Viewer

Length: 1005 bp
atgaagcaacgaatggtttatgcataccaccatgtggcgactggcattgcagcaaagctgacggcagaggaagtaaaagaaatggagacaaaagatgggtttatgtccgctcatctagataggattctgcctttgcacaccacccatagtccgaacttcttggggttataccaaggattgggactttggaaagggtctaattatggtgaaggtgtaattattggagttttggactctgggataacaccaggtcatccctcattcagcgatgaaggagtgccacctcatccggctaaatggaaaggtgagtgtgatttcaatgggacattctgcaataacaagtttattggtgccagaagtttccgaggtgggcaaactactggagatccaccaattgatgttgatggccatggtactcacacatccagcacagccggaggaaactttgtgtgcttttggaatggcaagtggaacagcttggtgcatttggagcaattcagaagggagtttttgtcagctgctcagcaggaaatggtttatggttcattgtccattgaagccccatggattctaacagtcggagcaagcactattgacagaagcataagagcaatatcaaaacttggaaatgggaaagaatatgatggggaatccaatgattcaacagctctgtgcgattcaggaacccttaatggagttgaagggaaagtggtgctgtgtgaaagaagtggtgaaattggaagaattgccagaggtgaagaagtcaaaagagcaggtgctgctgccatgatccttatgaacgaagaaacaaatggctatagcactttagctgatgctcatgtgcttcccgcaacccatgtgagttatgctgcaggtgcaggtatgagcatcaaagcttacataaacagtataaactccacctcaacacctacaaccacaatcttgttcaatggcactgtcatcggtgatcaaaatgcacctacagtcactaatttacatcaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

334

Amino Acids

35.75

Weight (kDa)

5.89

Isoelectric Point (pI)

29.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 764, 863
Acc36I ACCTGC 3 cut(s) 764, 863, 869
AccB1I GGYRCC 1 cut(s) 350
AccB7I CCANNNNNTGG 2 cut(s) 34, 178
AccBSI CCGCTC 1 cut(s) 110
AciI CCGC 2 cut(s) 108, 849
AclWI GGATC 2 cut(s) 380, 784
AcoI YGGCCR 1 cut(s) 406
AdeI CACNNNGTG 1 cut(s) 34
AfaI GTAC 1 cut(s) 415
AfiI CCNNNNNNNGG 2 cut(s) 34, 178
AgsI TTSAA 5 cut(s) 319, 557, 663, 701, 949
AjnI CCWGG 1 cut(s) 247
AluBI AGCT 6 cut(s) 58, 477, 518, 668, 830, 896
AluI AGCT 6 cut(s) 58, 477, 518, 668, 830, 896
Alw26I GTCTC 1 cut(s) 80
AlwI GGATC 2 cut(s) 380, 784
AlwNI CAGNNNCTG 1 cut(s) 779
AoxI GGCC 1 cut(s) 406
ApeKI GCWGC 5 cut(s) 50, 518, 779, 782, 869
AsuHPI GGTGA 5 cut(s) 218, 317, 743, 767, 977
BalI TGGCCA 1 cut(s) 408
BanI GGYRCC 1 cut(s) 350
BbvI GCAGC 5 cut(s) 62, 505, 766, 769, 856
BccI CCATC 3 cut(s) 89, 398, 638
BceAI ACGGC 1 cut(s) 78
BcgI CGANNNNNNTGC 2 cut(s) 943, 977
BciT130I CCWGG 1 cut(s) 249
BclI TGATCA 1 cut(s) 967
BcoDI GTCTC 1 cut(s) 80
BfaI CTAG 1 cut(s) 116
BfmI CTRYAG 3 cut(s) 817, 870, 981
BfuAI ACCTGC 3 cut(s) 764, 863, 869
BisI GCNGC 5 cut(s) 51, 519, 780, 783, 870
BlpI GCTNAGC 1 cut(s) 522
BlsI GCNGC 5 cut(s) 52, 520, 781, 784, 871
Bme1390I CCNGG 1 cut(s) 249
BmiI GGNNCC 2 cut(s) 352, 685
BmrFI CCNGG 1 cut(s) 249
BmsI GCATC 2 cut(s) 823, 897
BpmI CTGGAG 1 cut(s) 402
Bpu1102I GCTNAGC 1 cut(s) 522
BsaJI CCNNGG 4 cut(s) 172, 364, 409, 563
BsaXI ACNNNNNCTCC 2 cut(s) 497, 527
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 178
Bse1I ACTGG 2 cut(s) 46, 385
Bse3DI GCAATG 1 cut(s) 45
BseBI CCWGG 1 cut(s) 249
BseDI CCNNGG 4 cut(s) 172, 364, 409, 563
BseGI GGATG 3 cut(s) 253, 286, 422
BseLI CCNNNNNNNGG 2 cut(s) 34, 178
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 1 cut(s) 536
BseNI ACTGG 2 cut(s) 46, 385
BseXI GCAGC 5 cut(s) 62, 505, 766, 769, 856
BsgI GTGCAG 1 cut(s) 897
BshFI GGCC 1 cut(s) 408
BshNI GGYRCC 1 cut(s) 350
BsiSI CCGG 2 cut(s) 290, 435
BslFI GGGAC 2 cut(s) 195, 337
BslI CCNNNNNNNGG 2 cut(s) 34, 178
BsmAI GTCTC 1 cut(s) 80
BsmFI GGGAC 2 cut(s) 195, 337
BsnI GGCC 1 cut(s) 408
Bsp143I GATC 3 cut(s) 385, 789, 967
Bsp1720I GCTNAGC 1 cut(s) 522
Bsp19I CCATGG 2 cut(s) 409, 563
BspACI CCGC 2 cut(s) 108, 849
BspANI GGCC 1 cut(s) 408
BspCNI CTCAG 1 cut(s) 535
BspLI GGNNCC 2 cut(s) 352, 685
BspMAI CTGCAG 1 cut(s) 874
BspMI ACCTGC 3 cut(s) 764, 863, 869
BspPI GGATC 2 cut(s) 380, 784
BspT107I GGYRCC 1 cut(s) 350
BsrBI CCGCTC 1 cut(s) 110
BsrDI GCAATG 1 cut(s) 45
BsrI ACTGG 2 cut(s) 46, 385
BssECI CCNNGG 4 cut(s) 172, 364, 409, 563
BssMI GATC 3 cut(s) 385, 789, 967
BssT1I CCWWGG 3 cut(s) 172, 409, 563
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 4 cut(s) 577, 908, 958, 985
BstAPI GCANNNNNTGC 1 cut(s) 779
BstC8I GCNNGC 1 cut(s) 586
BstDEI CTNAG 1 cut(s) 522
BstDSI CCRYGG 2 cut(s) 409, 563
BstF5I GGATG 3 cut(s) 253, 286, 422
BstKTI GATC 3 cut(s) 388, 792, 970
BstMAI GTCTC 1 cut(s) 80
BstMBI GATC 3 cut(s) 385, 789, 967
BstMWI GCNNNNNNNGC 3 cut(s) 490, 779, 875
BstNI CCWGG 1 cut(s) 249
BstSCI CCNGG 1 cut(s) 247
BstSFI CTRYAG 3 cut(s) 817, 870, 981
BstV1I GCAGC 5 cut(s) 62, 505, 766, 769, 856
BstX2I RGATCY 1 cut(s) 385
BstYI RGATCY 1 cut(s) 385
BsuRI GGCC 1 cut(s) 408
BtgI CCRYGG 2 cut(s) 409, 563
BtgZI GCGATG 1 cut(s) 282
BtsCI GGATG 3 cut(s) 253, 286, 422
BtsIMutI CAGTG 1 cut(s) 954
BveI ACCTGC 3 cut(s) 764, 863, 869
Cac8I GCNNGC 1 cut(s) 586
CaiI CAGNNNCTG 1 cut(s) 779
CsiI ACCWGGT 1 cut(s) 247
Csp6I GTAC 1 cut(s) 414
CviAII CATG 6 cut(s) 32, 410, 564, 787, 839, 857
CviQI GTAC 1 cut(s) 414
DdeI CTNAG 1 cut(s) 522
DpnI GATC 3 cut(s) 387, 791, 969
DpnII GATC 3 cut(s) 385, 789, 967
DraIII CACNNNGTG 1 cut(s) 34
EaeI YGGCCR 1 cut(s) 406
Eco130I CCWWGG 3 cut(s) 172, 409, 563
EcoRII CCWGG 1 cut(s) 247
EcoT14I CCWWGG 3 cut(s) 172, 409, 563
EcoT22I ATGCAT 1 cut(s) 25
ErhI CCWWGG 3 cut(s) 172, 409, 563
FaeI CATG 6 cut(s) 35, 413, 567, 790, 842, 860
FaqI GGGAC 2 cut(s) 195, 337
FatI CATG 6 cut(s) 31, 409, 563, 786, 838, 856
FauI CCCGC 1 cut(s) 856
FbaI TGATCA 1 cut(s) 967
Fnu4HI GCNGC 5 cut(s) 51, 519, 780, 783, 870
FokI GGATG 3 cut(s) 240, 273, 409
Fsp4HI GCNGC 5 cut(s) 51, 519, 780, 783, 870
FspBI CTAG 1 cut(s) 116
GluI GCNGC 5 cut(s) 51, 519, 780, 783, 870
GsuI CTGGAG 1 cut(s) 402
HaeIII GGCC 1 cut(s) 408
HapII CCGG 2 cut(s) 290, 435
Hin1II CATG 6 cut(s) 35, 413, 567, 790, 842, 860
HindIII AAGCTT 1 cut(s) 894
HinfI GANTC 6 cut(s) 124, 233, 568, 650, 659, 677
HpaII CCGG 2 cut(s) 290, 435
HphI GGTGA 5 cut(s) 218, 317, 743, 767, 977
Hpy188I TCNGA 4 cut(s) 153, 365, 500, 581
Hpy188III TCNNGA 2 cut(s) 116, 681
HpyAV CCTTC 4 cut(s) 203, 266, 495, 695
HpyCH4III ACNGT 4 cut(s) 577, 908, 958, 985
HpyCH4V TGCA 8 cut(s) 23, 50, 136, 333, 484, 872, 878, 977
HpyF10VI GCNNNNNNNGC 3 cut(s) 490, 779, 875
HpyF3I CTNAG 1 cut(s) 522
Hsp92II CATG 6 cut(s) 35, 413, 567, 790, 842, 860
Ksp22I TGATCA 1 cut(s) 967
Kzo9I GATC 3 cut(s) 385, 789, 967
LmnI GCTCC 2 cut(s) 490, 581
Lsp1109I GCAGC 5 cut(s) 62, 505, 766, 769, 856
LweI GCATC 2 cut(s) 823, 897
MabI ACCWGGT 1 cut(s) 247
MaeI CTAG 1 cut(s) 116
MaeIII GTNAC 1 cut(s) 985
MalI GATC 3 cut(s) 387, 791, 969
MbiI CCGCTC 1 cut(s) 110
MboI GATC 3 cut(s) 385, 789, 967
MboII GAAGA 3 cut(s) 753, 770, 815
MfeI CAATTG 1 cut(s) 393
MflI RGATCY 1 cut(s) 385
MlsI TGGCCA 1 cut(s) 408
MluCI AATT 7 cut(s) 199, 216, 393, 494, 735, 744, 991
MluNI TGGCCA 1 cut(s) 408
MlyI GAGTC 1 cut(s) 227
MmeI TCCRAC 1 cut(s) 559
MnlI CCTC 7 cut(s) 61, 268, 294, 359, 431, 746, 931
Mox20I TGGCCA 1 cut(s) 408
Mph1103I ATGCAT 1 cut(s) 25
MscI TGGCCA 1 cut(s) 408
MseI TTAA 1 cut(s) 690
Msp20I TGGCCA 1 cut(s) 408
MspA1I CMGCKG 1 cut(s) 518
MspI CCGG 2 cut(s) 290, 435
MspR9I CCNGG 1 cut(s) 249
MunI CAATTG 1 cut(s) 393
MvaI CCWGG 1 cut(s) 249
MwoI GCNNNNNNNGC 3 cut(s) 490, 779, 875
NcoI CCATGG 2 cut(s) 409, 563
NdeII GATC 3 cut(s) 385, 789, 967
NlaIII CATG 6 cut(s) 35, 413, 567, 790, 842, 860
NlaIV GGNNCC 2 cut(s) 352, 685
NmuCI GTSAC 1 cut(s) 985
NsiI ATGCAT 1 cut(s) 25
PaqCI CACCTGC 2 cut(s) 764, 863
PfeI GAWTC 5 cut(s) 124, 568, 650, 659, 677
PflMI CCANNNNNTGG 2 cut(s) 34, 178
PkrI GCNGC 5 cut(s) 52, 520, 781, 784, 871
PleI GAGTC 1 cut(s) 227
PpsI GAGTC 1 cut(s) 227
Psp6I CCWGG 1 cut(s) 247
PspGI CCWGG 1 cut(s) 247
PspN4I GGNNCC 2 cut(s) 352, 685
PstI CTGCAG 1 cut(s) 874
PstNI CAGNNNCTG 1 cut(s) 779
PsuI RGATCY 1 cut(s) 385
PvuII CAGCTG 1 cut(s) 518
RsaI GTAC 1 cut(s) 415
RsaNI GTAC 1 cut(s) 414
SaqAI TTAA 1 cut(s) 690
SatI GCNGC 5 cut(s) 51, 519, 780, 783, 870
Sau3AI GATC 3 cut(s) 385, 789, 967
SchI GAGTC 1 cut(s) 227
ScrFI CCNGG 1 cut(s) 249
SexAI ACCWGGT 1 cut(s) 247
SfaNI GCATC 2 cut(s) 823, 897
SfcI CTRYAG 3 cut(s) 817, 870, 981
Sse9I AATT 7 cut(s) 199, 216, 393, 494, 735, 744, 991
SsiI CCGC 2 cut(s) 108, 849
SspMI CTAG 1 cut(s) 116
StyD4I CCNGG 1 cut(s) 247
StyI CCWWGG 3 cut(s) 172, 409, 563
TaaI ACNGT 4 cut(s) 577, 908, 958, 985
TasI AATT 7 cut(s) 199, 216, 393, 494, 735, 744, 991
TfiI GAWTC 5 cut(s) 124, 568, 650, 659, 677
Tru1I TTAA 1 cut(s) 690
Tru9I TTAA 1 cut(s) 690
TscAI CASTG 1 cut(s) 961
TseFI GTSAC 1 cut(s) 985
TseI GCWGC 5 cut(s) 50, 518, 779, 782, 869
Tsp45I GTSAC 1 cut(s) 985
TspDTI ATGAA 4 cut(s) 17, 285, 534, 812
TspRI CASTG 1 cut(s) 961
Van91I CCANNNNNTGG 2 cut(s) 34, 178
XbaI TCTAGA 1 cut(s) 115
XcmI CCANNNNNNNNNTGG 1 cut(s) 38
XspI CTAG 1 cut(s) 116
Zsp2I ATGCAT 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.