Prupe.5G085700_v2.0.a1

Subtilisin-like serine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000302
Physical Location & Seq
Forward (+)
9748427 .. 9750840
2414 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G085700.1

Sequence Viewer

Length: 2145 bp
ATGGAGAACAAAAAGTTTTTAGCTTTCCAGATAATATATCTTCTTGGCTTAAGTTTCATGTTGTATTTATCAATAGCAGTTGCCAATGAAGAGGCAGTGCTGGAACCGCTAGCTGAGACAAATGAGGAGCAGAATAACATGCAAACTTATATTGTTTGGGTGAAGAAGCCAGTCCAGAAATACTTTTTCTCAAAATCTCATGAAGATTTAGAGAGTTGGTATCACACATTTTTGCCCACAACTATAGCAAGCTCAAACCAGTTGAAGAAGCCACGAATGGTTCACGCATACCGCAACGTGGCCACTGGGTTTGCAGCAAAATTGACGCCAGAGGAAGTAAAGGCAATGGAAAATAAAGAAGGGTTTTTGTCCGCTCATCCAGAGCAAATTCTGCCTTTGCATACTACTCATAGTCCTAACTTCCTGGGGCTGCACCAAGGATTGGGAGTTTGGAAGGGATCAAACTATGGTGAAGGTGTGATCATTGGAGTTCTGGATACTGGGATATCACCTGATCATCCTTCATTTAGTGATGAAGGAGTAGCCCCTCCTCCAGCTAAATGGAAAGGCAAGTGCGACTTCAATGGAACAGTTTGCAATAATAAGCTTATTGGTGCAAGAAATTTCCAAGGTGGCCAAACCACGGGAGGCCCTCCAGTTGACGATGAAGGTCATGGCACCCACACATCCAGCACAGCTGCAGGAAATTTTGTGAAAGGTGCCAATGTGTTTGGAATGGCCAATGGCACAGCGTCTGGCATGGCACCTTATGCTCACCTGGCAATTTACAAGGTCTGTTCTGAGGAGGGTTGTGCTGAAGGTGACATCTTAGCTGCTCTTGATACTGCTGTGGAAGATGGTGTGGACGTGCTCTCCCTCTCACTCGGAGGTGCCTCAGTTCCTTTTTATGCTGATGGGATTGCAATTGGTGCATTTGGAGCAATACAAAAGGGAATTTTTGTCAGTTGTTCAGCCGGAAATTCGGGTCCTTTCTATGCTTCTTTAGCAAATGAGGCACCCTGGATTCTCACAGTTGGAGCAAGCACCATTGACAGAAGCATAAAAGCGACAGCATTGCTTGGAAATGGAGCAGAATATGATGGTGAATCATTATTCCAGCCAAAAGATTTTAGTTCAAAATTATTACCTCTTGTTTATGCAGGCGCAAATGGAAAACAATCATCAGCATTCTGTGATGCTGGATCACTTGGAAACGTTGAAGGGGCAATAGTGTTGTGTGAGAGAGGTGGAGGAGTAGCAAGAATTGACAAAGGGGCAGAAGTGAAAAGAGCTGGTGGAGCTGCCATGATTCTCGTGAATGCAGAGACTGATGGTGATAGCACCTTAGCTGACCCTCATGTACTTCCCGCAACACACGTGGGTTATGTTGCAGGTGTTAAAATCAAAGCCTATCTGAATTCCACCTCAAGTCCTGCAGCCACAATCTTGTTCAAAGGAACTGTCATTGGAGACGGACTTGCTCCCAAGATTACTTCCTTTTCATCAAGAGGACCAAGCATTGCAAGCACAGGAATTTTGAAACCTGACATCATTGGTCCCGGTGTTAGCATCCTAGCAGCATGGCCTGTTTCGGTGGACAACGGCACAGAAGACGAAACACTTAAGCCAGCAGACATCTTTGCCACTGGTGCAGGCCATGTTAACCCTTCAAAAGCAAATGACCCTGGGCTCATCTATGACACAAAACCAGAGGATTACATTCCTTACTTGTGTGGTTTGAATTACACAGACGAACAGATACAGGTCATCACCCAACAAAGAGTGAACTGCTCTCAAGTAGAAGCCATACCAGAAGCACAGCTAAACTATCCTTCATTCTCCATTATAGTAGGGTCGTCCGAAGACTCTAAATCTCAGTATTATACAAGGACCGTCAAGAATGTTGGCCCGGCTAATTCAACTTACAACTTGGATCTTTTTGTGCCACGGAACATGGGCATGAGTGTGAACCCTGAGGTGCTTAAATTCACAGAGGTTAACCAGGAGATTACATTCGAAGCGGAGTTTTTCGCAGAAGACAGTGCTGGGAAGGATGGTGTACCATTTGCTCAGGGATATTTGAGATGGGTTTCTGATCAGCATTCTGTTACTAGCCCAATATCTGTTATCTTTGCCAGCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

715

Amino Acids

75.65

Weight (kDa)

5.04

Isoelectric Point (pI)

29.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1382
Acc36I ACCTGC 1 cut(s) 1382
AccB1I GGYRCC 5 cut(s) 677, 719, 763, 890, 1015
AccB7I CCANNNNNTGG 1 cut(s) 442
AccBSI CCGCTC 1 cut(s) 374
AciI CCGC 5 cut(s) 107, 292, 372, 1368, 2021
AclI AACGTT 1 cut(s) 1215
AclWI GGATC 3 cut(s) 466, 1210, 1941
AcoI YGGCCR 3 cut(s) 300, 634, 738
AcsI RAATTY 8 cut(s) 387, 622, 706, 954, 979, 1417, 1533, 1985
AcuI CTGAAG 1 cut(s) 837
AcvI CACGTG 1 cut(s) 1378
AcyI GRCGYC 1 cut(s) 326
AfaI GTAC 2 cut(s) 1362, 2061
AfiI CCNNNNNNNGG 3 cut(s) 298, 442, 643
AflII CTTAAG 2 cut(s) 49, 1622
AflIII ACRYGT 1 cut(s) 1375
AgsI TTSAA 9 cut(s) 265, 583, 1137, 1220, 1453, 1540, 1671, 1741, 1920
AjiI CACGTC 1 cut(s) 868
AjnI CCWGG 5 cut(s) 423, 777, 1019, 1684, 2001
Alw21I GWGCWC 1 cut(s) 873
Alw26I GTCTC 3 cut(s) 110, 1319, 1464
AlwI GGATC 3 cut(s) 466, 1210, 1941
AlwNI CAGNNNCTG 2 cut(s) 755, 1328
AoxI GGCC 7 cut(s) 300, 634, 649, 738, 1583, 1654, 1906
ApeKI GCWGC 7 cut(s) 314, 430, 698, 833, 1301, 1436, 1577
ApoI RAATTY 8 cut(s) 387, 622, 706, 954, 979, 1417, 1533, 1985
ArsI GACNNNNNNTTYG 2 cut(s) 1446, 1478
AspLEI GCGC 1 cut(s) 1166
AspS9I GGNCC 6 cut(s) 650, 986, 1511, 1556, 1890, 1907
AsuC2I CCSGG 2 cut(s) 1560, 1910
AsuHPI GGTGA 8 cut(s) 172, 482, 501, 767, 833, 1115, 1346, 1762
AsuII TTCGAA 1 cut(s) 2016
AsuNHI GCTAGC 1 cut(s) 109
AvaII GGWCC 4 cut(s) 986, 1511, 1556, 1890
AxyI CCTNAGG 1 cut(s) 1974
BaeI ACNNNNGTAYC 2 cut(s) 834, 867
BalI TGGCCA 3 cut(s) 302, 636, 740
BanI GGYRCC 5 cut(s) 677, 719, 763, 890, 1015
BanII GRGCYC 1 cut(s) 1692
BauI CACGAG 1 cut(s) 1313
BbrPI CACGTG 1 cut(s) 1378
BbsI GAAGAC 3 cut(s) 1617, 1869, 2043
Bbv12I GWGCWC 1 cut(s) 873
BbvI GCAGC 7 cut(s) 326, 417, 685, 820, 1288, 1448, 1589
BccI CCATC 6 cut(s) 851, 908, 1094, 1325, 2048, 2079
BceAI ACGGC 1 cut(s) 1618
BcgI CGANNNNNNTGC 2 cut(s) 1057, 1091
BciT130I CCWGG 5 cut(s) 425, 779, 1021, 1686, 2003
BciVI GTATCC 1 cut(s) 490
BclI TGATCA 3 cut(s) 480, 514, 2095
BcnI CCSGG 2 cut(s) 1560, 1910
BcoDI GTCTC 3 cut(s) 110, 1319, 1464
BfaI CTAG 3 cut(s) 110, 1574, 2112
BfmI CTRYAG 3 cut(s) 243, 699, 1434
BfrI CTTAAG 2 cut(s) 49, 1622
BfuAI ACCTGC 1 cut(s) 1382
BfuI GTATCC 1 cut(s) 490
BisI GCNGC 7 cut(s) 315, 431, 699, 834, 1302, 1437, 1578
BlsI GCNGC 7 cut(s) 316, 432, 700, 835, 1303, 1438, 1579
Bme1390I CCNGG 7 cut(s) 425, 779, 1021, 1560, 1686, 1910, 2003
Bme18I GGWCC 4 cut(s) 986, 1511, 1556, 1890
BmgBI CACGTC 1 cut(s) 868
BmgT120I GGNCC 6 cut(s) 650, 986, 1511, 1556, 1890, 1907
BmiI GGNNCC 8 cut(s) 105, 679, 721, 765, 892, 987, 1017, 1558
BmrFI CCNGG 7 cut(s) 425, 779, 1021, 1560, 1686, 1910, 2003
BmrI ACTGGG 2 cut(s) 315, 510
BmsI GCATC 2 cut(s) 1186, 1578
BmtI GCTAGC 1 cut(s) 113
BmuI ACTGGG 2 cut(s) 315, 510
BpiI GAAGAC 3 cut(s) 1617, 1869, 2043
BpmI CTGGAG 2 cut(s) 537, 639
Bpu10I CCTNAGC 2 cut(s) 1345, 2070
Bpu14I TTCGAA 1 cut(s) 2016
BpuEI CTTGAG 2 cut(s) 1411, 1779
BpuMI CCSGG 2 cut(s) 1560, 1910
BsaAI YACGTR 1 cut(s) 1378
BsaHI GRCGYC 1 cut(s) 326
BsaJI CCNNGG 8 cut(s) 424, 436, 628, 642, 1019, 1684, 1685, 1946
BsaXI ACNNNNNCTCC 2 cut(s) 857, 887
Bsc4I CCNNNNNNNGG 3 cut(s) 298, 442, 643
Bse1I ACTGG 6 cut(s) 170, 259, 310, 505, 656, 1651
Bse21I CCTNAGG 1 cut(s) 1974
Bse3DI GCAATG 3 cut(s) 351, 1073, 1518
BseBI CCWGG 5 cut(s) 425, 779, 1021, 1686, 2003
BseDI CCNNGG 8 cut(s) 424, 436, 628, 642, 1019, 1684, 1685, 1946
BseGI GGATG 5 cut(s) 376, 517, 686, 1569, 2059
BseLI CCNNNNNNNGG 3 cut(s) 298, 442, 643
BseMI GCAATG 3 cut(s) 351, 1073, 1518
BseMII CTCAG 6 cut(s) 105, 792, 909, 1889, 1965, 2084
BseNI ACTGG 6 cut(s) 170, 259, 310, 505, 656, 1651
BseRI GAGGAG 4 cut(s) 140, 540, 818, 1266
BseXI GCAGC 7 cut(s) 326, 417, 685, 820, 1288, 1448, 1589
BseYI CCCAGC 1 cut(s) 2045
BsgI GTGCAG 2 cut(s) 416, 1671
BshFI GGCC 7 cut(s) 302, 636, 651, 740, 1585, 1656, 1908
BshNI GGYRCC 5 cut(s) 677, 719, 763, 890, 1015
BsiHKAI GWGCWC 1 cut(s) 873
BsiSI CCGG 3 cut(s) 975, 1560, 1910
BslFI GGGAC 1 cut(s) 1542
BslI CCNNNNNNNGG 3 cut(s) 298, 442, 643
BsmAI GTCTC 3 cut(s) 110, 1319, 1464
BsmBI CGTCTC 1 cut(s) 1464
BsmFI GGGAC 1 cut(s) 1542
BsmI GAATGC 3 cut(s) 1187, 1324, 2101
BsnI GGCC 7 cut(s) 302, 636, 651, 740, 1585, 1656, 1908
Bsp119I TTCGAA 1 cut(s) 2016
Bsp1286I GDGCHC 2 cut(s) 873, 1692
Bsp143I GATC 6 cut(s) 458, 480, 514, 1202, 1933, 2095
BspACI CCGC 5 cut(s) 107, 292, 372, 1368, 2021
BspANI GGCC 7 cut(s) 302, 636, 651, 740, 1585, 1656, 1908
BspCNI CTCAG 6 cut(s) 106, 793, 908, 1888, 1966, 2083
BspHI TCATGA 1 cut(s) 199
BspLI GGNNCC 8 cut(s) 105, 679, 721, 765, 892, 987, 1017, 1558
BspMAI CTGCAG 2 cut(s) 703, 1438
BspMI ACCTGC 1 cut(s) 1382
BspOI GCTAGC 1 cut(s) 113
BspPI GGATC 3 cut(s) 466, 1210, 1941
BspT104I TTCGAA 1 cut(s) 2016
BspT107I GGYRCC 5 cut(s) 677, 719, 763, 890, 1015
BspTI CTTAAG 2 cut(s) 49, 1622
BsrBI CCGCTC 1 cut(s) 374
BsrDI GCAATG 3 cut(s) 351, 1073, 1518
BsrI ACTGG 6 cut(s) 170, 259, 310, 505, 656, 1651
BssECI CCNNGG 8 cut(s) 424, 436, 628, 642, 1019, 1684, 1685, 1946
BssMI GATC 6 cut(s) 458, 480, 514, 1202, 1933, 2095
BssNI GRCGYC 1 cut(s) 326
BssSI CACGAG 1 cut(s) 1313
BssT1I CCWWGG 2 cut(s) 436, 628
Bst2BI CACGAG 1 cut(s) 1313
Bst2UI CCWGG 5 cut(s) 425, 779, 1021, 1686, 2003
Bst4CI ACNGT 5 cut(s) 592, 1033, 1462, 1894, 2042
Bst6I CTCTTC 1 cut(s) 84
BstACI GRCGYC 1 cut(s) 326
BstAFI CTTAAG 2 cut(s) 49, 1622
BstAPI GCANNNNNTGC 3 cut(s) 391, 770, 929
BstBAI YACGTR 1 cut(s) 1378
BstBI TTCGAA 1 cut(s) 2016
BstC8I GCNNGC 8 cut(s) 111, 250, 1042, 1162, 1525, 1629, 1654, 2137
BstDEI CTNAG 8 cut(s) 114, 801, 829, 895, 1345, 1875, 1974, 2070
BstDSI CCRYGG 2 cut(s) 642, 1946
BstF5I GGATG 5 cut(s) 376, 517, 686, 1569, 2059
BstHHI GCGC 1 cut(s) 1166
BstKTI GATC 6 cut(s) 461, 483, 517, 1205, 1936, 2098
BstMAI GTCTC 3 cut(s) 110, 1319, 1464
BstMBI GATC 6 cut(s) 458, 480, 514, 1202, 1933, 2095
BstNI CCWGG 5 cut(s) 425, 779, 1021, 1686, 2003
BstNSI RCATGY 1 cut(s) 142
BstSCI CCNGG 7 cut(s) 423, 777, 1019, 1558, 1684, 1908, 2001
BstSFI CTRYAG 3 cut(s) 243, 699, 1434
BstV1I GCAGC 7 cut(s) 326, 417, 685, 820, 1288, 1448, 1589
BstV2I GAAGAC 3 cut(s) 1617, 1869, 2043
BstX2I RGATCY 1 cut(s) 1933
BstXI CCANNNNNNTGG 1 cut(s) 561
BstYI RGATCY 1 cut(s) 1933
Bsu36I CCTNAGG 1 cut(s) 1974
BsuI GTATCC 1 cut(s) 490
BsuRI GGCC 7 cut(s) 302, 636, 651, 740, 1585, 1656, 1908
BtgI CCRYGG 2 cut(s) 642, 1946
BtrI CACGTC 1 cut(s) 868
BtsCI GGATG 5 cut(s) 376, 517, 686, 1569, 2059
BtsI GCAGTG 1 cut(s) 102
BtsIMutI CAGTG 4 cut(s) 102, 303, 1644, 2047
BveI ACCTGC 1 cut(s) 1382
Cac8I GCNNGC 8 cut(s) 111, 250, 1042, 1162, 1525, 1629, 1654, 2137
CaiI CAGNNNCTG 2 cut(s) 755, 1328
CciI TCATGA 1 cut(s) 199
CfoI GCGC 1 cut(s) 1166
Cfr13I GGNCC 6 cut(s) 650, 986, 1511, 1556, 1890, 1907
CseI GACGC 2 cut(s) 334, 741
Csp6I GTAC 2 cut(s) 1361, 2060
CspCI CAANNNNNGTGG 4 cut(s) 292, 327, 1359, 1394
CviQI GTAC 2 cut(s) 1361, 2060
DdeI CTNAG 8 cut(s) 114, 801, 829, 895, 1345, 1875, 1974, 2070
DpnI GATC 6 cut(s) 460, 482, 516, 1204, 1935, 2097
DpnII GATC 6 cut(s) 458, 480, 514, 1202, 1933, 2095
EaeI YGGCCR 3 cut(s) 300, 634, 738
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
Eco130I CCWWGG 2 cut(s) 436, 628
Eco24I GRGCYC 1 cut(s) 1692
Eco32I GATATC 1 cut(s) 507
Eco47I GGWCC 4 cut(s) 986, 1511, 1556, 1890
Eco57I CTGAAG 1 cut(s) 837
Eco72I CACGTG 1 cut(s) 1378
Eco81I CCTNAGG 1 cut(s) 1974
EcoO109I RGGNCCY 2 cut(s) 650, 986
EcoRI GAATTC 1 cut(s) 1417
EcoRII CCWGG 5 cut(s) 423, 777, 1019, 1684, 2001
EcoRV GATATC 1 cut(s) 507
EcoT14I CCWWGG 2 cut(s) 436, 628
EcoT38I GRGCYC 1 cut(s) 1692
ErhI CCWWGG 2 cut(s) 436, 628
Esp3I CGTCTC 1 cut(s) 1464
FalI AAGNNNNNCTT 2 cut(s) 563, 595
FaqI GGGAC 1 cut(s) 1542
FauI CCCGC 1 cut(s) 1375
FbaI TGATCA 3 cut(s) 480, 514, 2095
Fnu4HI GCNGC 7 cut(s) 315, 431, 699, 834, 1302, 1437, 1578
FokI GGATG 5 cut(s) 363, 504, 673, 1556, 2066
FriOI GRGCYC 1 cut(s) 1692
Fsp4HI GCNGC 7 cut(s) 315, 431, 699, 834, 1302, 1437, 1578
FspBI CTAG 3 cut(s) 110, 1574, 2112
GlaI GCGC 1 cut(s) 1165
GluI GCNGC 7 cut(s) 315, 431, 699, 834, 1302, 1437, 1578
GsaI CCCAGC 1 cut(s) 2049
GsuI CTGGAG 2 cut(s) 537, 639
HaeIII GGCC 7 cut(s) 302, 636, 651, 740, 1585, 1656, 1908
HapII CCGG 3 cut(s) 975, 1560, 1910
HgaI GACGC 2 cut(s) 334, 741
HhaI GCGC 1 cut(s) 1166
Hin1I GRCGYC 1 cut(s) 326
Hin6I GCGC 1 cut(s) 1164
HinP1I GCGC 1 cut(s) 1164
HincII GTYRAC 3 cut(s) 661, 1663, 1999
HindII GTYRAC 3 cut(s) 661, 1663, 1999
HindIII AAGCTT 1 cut(s) 605
HinfI GANTC 4 cut(s) 1024, 1106, 1309, 1865
HpaI GTTAAC 2 cut(s) 1663, 1999
HpaII CCGG 3 cut(s) 975, 1560, 1910
HphI GGTGA 8 cut(s) 172, 482, 501, 767, 833, 1115, 1346, 1762
Hpy166II GTNNAC 9 cut(s) 283, 661, 865, 1597, 1663, 1786, 1969, 1999, 2060
Hpy188I TCNGA 5 cut(s) 802, 887, 1416, 1861, 2095
Hpy188III TCNNGA 9 cut(s) 28, 175, 200, 380, 494, 839, 1315, 1506, 1897
Hpy8I GTNNAC 9 cut(s) 283, 661, 865, 1597, 1663, 1786, 1969, 1999, 2060
HpyCH4III ACNGT 5 cut(s) 592, 1033, 1462, 1894, 2042
HpyCH4IV ACGT 4 cut(s) 297, 867, 1215, 1377
HpyF3I CTNAG 8 cut(s) 114, 801, 829, 895, 1345, 1875, 1974, 2070
HpySE526I ACGT 4 cut(s) 297, 867, 1215, 1377
Hsp92I GRCGYC 1 cut(s) 326
HspAI GCGC 1 cut(s) 1164
Ksp22I TGATCA 3 cut(s) 480, 514, 2095
KspAI GTTAAC 2 cut(s) 1663, 1999
Kzo9I GATC 6 cut(s) 458, 480, 514, 1202, 1933, 2095
LmnI GCTCC 6 cut(s) 127, 938, 1037, 1088, 1298, 1486
Lsp1109I GCAGC 7 cut(s) 326, 417, 685, 820, 1288, 1448, 1589
LweI GCATC 2 cut(s) 1186, 1578
MaeI CTAG 3 cut(s) 110, 1574, 2112
MaeII ACGT 4 cut(s) 297, 867, 1215, 1377
MaeIII GTNAC 2 cut(s) 821, 2107
MalI GATC 6 cut(s) 460, 482, 516, 1204, 1935, 2097
MbiI CCGCTC 1 cut(s) 374
MboI GATC 6 cut(s) 458, 480, 514, 1202, 1933, 2095
MboII GAAGA 9 cut(s) 32, 101, 175, 215, 277, 866, 1622, 1874, 2048
MfeI CAATTG 1 cut(s) 924
MflI RGATCY 1 cut(s) 1933
MhlI GDGCHC 2 cut(s) 873, 1692
MlsI TGGCCA 3 cut(s) 302, 636, 740
MluNI TGGCCA 3 cut(s) 302, 636, 740
MlyI GAGTC 1 cut(s) 1859
MmeI TCCRAC 1 cut(s) 1015
Mox20I TGGCCA 3 cut(s) 302, 636, 740
MscI TGGCCA 3 cut(s) 302, 636, 740
MseI TTAA 6 cut(s) 50, 1398, 1623, 1662, 1983, 1998
MslI CAYNNNNRTG 2 cut(s) 1958, 1964
Msp20I TGGCCA 3 cut(s) 302, 636, 740
MspA1I CMGCKG 1 cut(s) 698
MspCI CTTAAG 2 cut(s) 49, 1622
MspI CCGG 3 cut(s) 975, 1560, 1910
MspR9I CCNGG 7 cut(s) 425, 779, 1021, 1560, 1686, 1910, 2003
MunI CAATTG 1 cut(s) 924
Mva1269I GAATGC 3 cut(s) 1187, 1324, 2101
MvaI CCWGG 5 cut(s) 425, 779, 1021, 1686, 2003
NciI CCSGG 2 cut(s) 1560, 1910
NdeII GATC 6 cut(s) 458, 480, 514, 1202, 1933, 2095
NheI GCTAGC 1 cut(s) 109
NlaIV GGNNCC 8 cut(s) 105, 679, 721, 765, 892, 987, 1017, 1558
NmuCI GTSAC 1 cut(s) 821
NspI RCATGY 1 cut(s) 142
NspV TTCGAA 1 cut(s) 2016
PagI TCATGA 1 cut(s) 199
PaqCI CACCTGC 1 cut(s) 1382
PasI CCCWGGG 1 cut(s) 1685
PctI GAATGC 3 cut(s) 1187, 1324, 2101
PfeI GAWTC 3 cut(s) 1024, 1106, 1309
PflMI CCANNNNNTGG 1 cut(s) 442
PkrI GCNGC 7 cut(s) 316, 432, 700, 835, 1303, 1438, 1579
PleI GAGTC 1 cut(s) 1859
PmaCI CACGTG 1 cut(s) 1378
PmlI CACGTG 1 cut(s) 1378
PpsI GAGTC 1 cut(s) 1859
Ppu21I YACGTR 1 cut(s) 1378
PpuMI RGGWCCY 1 cut(s) 986
Psp1406I AACGTT 1 cut(s) 1215
Psp5II RGGWCCY 1 cut(s) 986
Psp6I CCWGG 5 cut(s) 423, 777, 1019, 1684, 2001
PspCI CACGTG 1 cut(s) 1378
PspFI CCCAGC 1 cut(s) 2045
PspGI CCWGG 5 cut(s) 423, 777, 1019, 1684, 2001
PspN4I GGNNCC 8 cut(s) 105, 679, 721, 765, 892, 987, 1017, 1558
PspPI GGNCC 6 cut(s) 650, 986, 1511, 1556, 1890, 1907
PspPPI RGGWCCY 1 cut(s) 986
PstI CTGCAG 2 cut(s) 703, 1438
PstNI CAGNNNCTG 2 cut(s) 755, 1328
PsuI RGATCY 1 cut(s) 1933
PvuII CAGCTG 1 cut(s) 698
RsaI GTAC 2 cut(s) 1362, 2061
RsaNI GTAC 2 cut(s) 1361, 2060
RseI CAYNNNNRTG 2 cut(s) 1958, 1964
SaqAI TTAA 6 cut(s) 50, 1398, 1623, 1662, 1983, 1998
SatI GCNGC 7 cut(s) 315, 431, 699, 834, 1302, 1437, 1578
Sau3AI GATC 6 cut(s) 458, 480, 514, 1202, 1933, 2095
Sau96I GGNCC 6 cut(s) 650, 986, 1511, 1556, 1890, 1907
SchI GAGTC 1 cut(s) 1859
ScrFI CCNGG 7 cut(s) 425, 779, 1021, 1560, 1686, 1910, 2003
SduI GDGCHC 2 cut(s) 873, 1692
SfaNI GCATC 2 cut(s) 1186, 1578
SfcI CTRYAG 3 cut(s) 243, 699, 1434
SfuI TTCGAA 1 cut(s) 2016
SinI GGWCC 4 cut(s) 986, 1511, 1556, 1890
SmiMI CAYNNNNRTG 2 cut(s) 1958, 1964
SmlI CTYRAG 4 cut(s) 49, 1426, 1622, 1794
SmoI CTYRAG 4 cut(s) 49, 1426, 1622, 1794
SsiI CCGC 5 cut(s) 107, 292, 372, 1368, 2021
SspMI CTAG 3 cut(s) 110, 1574, 2112
StyD4I CCNGG 7 cut(s) 423, 777, 1019, 1558, 1684, 1908, 2001
StyI CCWWGG 2 cut(s) 436, 628
TaaI ACNGT 5 cut(s) 592, 1033, 1462, 1894, 2042
TaiI ACGT 4 cut(s) 300, 870, 1218, 1380
TaqI TCGA 1 cut(s) 2016
TatI WGTACW 1 cut(s) 1360
TfiI GAWTC 3 cut(s) 1024, 1106, 1309
Tru1I TTAA 6 cut(s) 50, 1398, 1623, 1662, 1983, 1998
Tru9I TTAA 6 cut(s) 50, 1398, 1623, 1662, 1983, 1998
TscAI CASTG 4 cut(s) 102, 310, 1651, 2047
TseFI GTSAC 1 cut(s) 821
TseI GCWGC 7 cut(s) 314, 430, 698, 833, 1301, 1436, 1577
Tsp45I GTSAC 1 cut(s) 821
TspDTI ATGAA 8 cut(s) 46, 102, 216, 513, 549, 681, 1491, 1824
TspGWI ACGGA 2 cut(s) 1488, 1963
TspRI CASTG 4 cut(s) 102, 310, 1651, 2047
Van91I CCANNNNNTGG 1 cut(s) 442
Vha464I CTTAAG 2 cut(s) 49, 1622
VpaK11BI GGWCC 4 cut(s) 986, 1511, 1556, 1890
XapI RAATTY 8 cut(s) 387, 622, 706, 954, 979, 1417, 1533, 1985
XceI RCATGY 1 cut(s) 142
XspI CTAG 3 cut(s) 110, 1574, 2112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.