Rh6CG145600

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000302
Physical Location & Seq
Forward (+)
19018464 .. 19021650
3187 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG145600.1

Sequence Viewer

Length: 1530 bp
ATGTTTTACATGTCACTAGTAGTTGCTGATGAAAAAGCGGCAGCATTAGAGTCACTAGCCAAGACAAAGAGCATGCAAACTTATATAGTTTGGGTGGAAAAGCCAATCAGCGATTTGATTGCACAATCTGAACATGAAGATTTAGGGAGTTGGTACCAGACATTTTTGCCCCAAACTATTGCAAACTCAAACCAGCTTGAGAAGCCACGAATGGTTTATGCATACCACAATGTGGCAACTGGCTTCGCAGCAAAGCTGACAGCAAAGGAAGTTGAAGAGATGGAAAAGAAAGATGGGTTTATCTCCGCTCATCCAGAACGAAATCTGCAATTGCACACAACTCATAGTCCTAACTTCTTGGGGCTGCAGCAAGGAATGGGACTAGGTGCACATAGCAATGATTCATCAGCTCTTTGCGATGTAGGGTCCCTTAAATATGTTGAAGGGAAAGTAGTGGTGTGTGAGGTAGGTGGAGGAATTGGAAGAATTGCAAAAGGGGTAGAAGTGAAAAGAGCTGGTGGTGTTGCCATGATTCTTGTCAACCCAGACTTCGCTGGCTATACCACCATAGCAGACATTCACGTGCTTCCGGCAACACATGTGAGTTACTCTGCAGGGGTGAGCATCAAAACCTATATAAATTCAACCTCAACTCCTACAGCGACAATCTTATTCAAAGGCACTATCATCGGTGATCAGCTTGCTCCCAAGGTTGCTTTCTTTTCATCAAGAGGACCAAGCCTGGCAAGTCCTGCAATATTGAAACCTGACATTATTGGTCCCGGTGTGAGCATCCTAGCTGCGTGGCCTTTTTCAGTGGATAATGCTTTAGAATCTAAGGCCACATTTGACATTATTTCAGGTACTTCAATGTCATGCCCTCACCTAAGTGGCATTGCAGCCTTGCTCAAGAGCTCACACCCTGACTGGTCACCTGCTGCCATTAAGTCTGCAATGATGACAACTGCTGAAGTAAACAACCTCGCTGGCTCGGCCATTCTTGATCAAACACTTCTTGCAGCAGACCTCTTTGCCATTGGCGCAGGCCATGTTAACCCTTCAAAAGCAAATGACCCTGGGCTCATCTACGACATACAACCAGAGGACTACATTCCTTACTTGTGTGGTTTGAATTACACAAGCAAACAGATATCGGCAATCACCCAACAAAAAGTGTCGTGCTCTAAAGTAGGAGTCATACCAGAAGCACAGCTAAACTATCCCACATTTTGTATCTATATAGAGTCTGGTGGTGAGCCTCAGAATTACACAAGAACCTTGACCAATGTTGGCCCAGCTAATTCAACTTACAAATTGGCTCCCCTAAGACTACATAAAATGGACATCACTGTGCTACCTGAGGTGCTTACATTTACAGAGGTTAACCAAAAATTGACATACCGTGTGGTGTTTGCCGCACAAGATGGAGCTGGGAAAGATGGTATACCATTTTCTCAGGGATATTTGAGTTGGGTGTCTCATCAGCATTCTGTTAACAGCCAAATATCTGTGGTCTTTGACTATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

509

Amino Acids

54.66

Weight (kDa)

5.87

Isoelectric Point (pI)

33.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 27 - 112 4.8e-11 Peptidase inhibitor I9
PA PF02225 146 - 208 2.8e-09 PA domain
Peptidase_S8 PF00082 233 - 326 9.4e-18 Subtilase family
fn3_6 PF17766 404 - 504 2.1e-21 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 943
Acc36I ACCTGC 1 cut(s) 943
Acc65I GGTACC 1 cut(s) 153
AccB1I GGYRCC 1 cut(s) 153
AccB7I CCANNNNNTGG 1 cut(s) 232
AccBSI CCGCTC 1 cut(s) 308
AccI GTMKAC 1 cut(s) 1444
AciI CCGC 3 cut(s) 38, 306, 1416
AcoI YGGCCR 1 cut(s) 993
AcsI RAATTY 1 cut(s) 640
AcuI CTGAAG 1 cut(s) 990
AcvI CACGTG 1 cut(s) 583
AdeI CACNNNGTG 1 cut(s) 232
AfaI GTAC 2 cut(s) 155, 865
AfiI CCNNNNNNNGG 1 cut(s) 232
AflIII ACRYGT 2 cut(s) 9, 598
AgsI TTSAA 9 cut(s) 275, 443, 645, 676, 763, 870, 1062, 1132, 1305
AhlI ACTAGT 1 cut(s) 16
AjnI CCWGG 2 cut(s) 741, 1075
AjuI GAANNNNNNNTTGG 2 cut(s) 701, 733
AleI CACNNNNGTG 1 cut(s) 888
Alw21I GWGCWC 3 cut(s) 391, 917, 1184
Alw26I GTCTC 1 cut(s) 1482
Alw44I GTGCAC 1 cut(s) 387
AoxI GGCC 5 cut(s) 806, 840, 993, 1045, 1291
ApaLI GTGCAC 1 cut(s) 387
ApeKI GCWGC 8 cut(s) 41, 248, 364, 367, 800, 899, 938, 1019
ApoI RAATTY 1 cut(s) 640
Asp718I GGTACC 1 cut(s) 153
AspLEI GCGC 1 cut(s) 1043
AspS9I GGNCC 4 cut(s) 426, 734, 779, 1292
AsuC2I CCSGG 1 cut(s) 783
AsuHPI GGTGA 6 cut(s) 631, 704, 875, 924, 1153, 1265
AvaII GGWCC 3 cut(s) 426, 734, 779
AxyI CCTNAGG 1 cut(s) 1359
BaeGI GKGCMC 1 cut(s) 391
BanI GGYRCC 1 cut(s) 153
BanII GRGCYC 2 cut(s) 917, 1083
BbrPI CACGTG 1 cut(s) 583
Bbv12I GWGCWC 3 cut(s) 391, 917, 1184
BbvI GCAGC 8 cut(s) 53, 260, 351, 379, 787, 911, 925, 1031
BccI CCATC 4 cut(s) 274, 287, 1418, 1433
BcgI CGANNNNNNTGC 4 cut(s) 101, 135, 670, 704
BciT130I CCWGG 2 cut(s) 743, 1077
BclI TGATCA 2 cut(s) 694, 1003
BcnI CCSGG 1 cut(s) 783
BcoDI GTCTC 1 cut(s) 1482
BcuI ACTAGT 1 cut(s) 16
BfaI CTAG 4 cut(s) 17, 56, 383, 797
BfmI CTRYAG 3 cut(s) 365, 612, 657
BfuAI ACCTGC 1 cut(s) 943
Bme1390I CCNGG 3 cut(s) 743, 783, 1077
Bme18I GGWCC 3 cut(s) 426, 734, 779
BmgT120I GGNCC 4 cut(s) 426, 734, 779, 1292
BmiI GGNNCC 5 cut(s) 155, 427, 428, 781, 1320
BmrFI CCNGG 3 cut(s) 743, 783, 1077
BmsI GCATC 2 cut(s) 633, 801
BpuEI CTTGAG 2 cut(s) 218, 893
BpuMI CCSGG 1 cut(s) 783
BsaAI YACGTR 1 cut(s) 583
BsaJI CCNNGG 3 cut(s) 708, 1075, 1076
BsaXI ACNNNNNCTCC 2 cut(s) 637, 667
Bsc4I CCNNNNNNNGG 1 cut(s) 232
Bse1I ACTGG 2 cut(s) 244, 932
Bse21I CCTNAGG 1 cut(s) 1359
Bse3DI GCAATG 3 cut(s) 403, 894, 960
BseBI CCWGG 2 cut(s) 743, 1077
BseDI CCNNGG 3 cut(s) 708, 1075, 1076
BseGI GGATG 2 cut(s) 310, 792
BseLI CCNNNNNNNGG 1 cut(s) 232
BseMI GCAATG 3 cut(s) 403, 894, 960
BseMII CTCAG 3 cut(s) 1274, 1350, 1469
BseNI ACTGG 2 cut(s) 244, 932
BseSI GKGCMC 1 cut(s) 391
BseXI GCAGC 8 cut(s) 53, 260, 351, 379, 787, 911, 925, 1031
BseYI CCCAGC 2 cut(s) 1294, 1430
BshFI GGCC 5 cut(s) 808, 842, 995, 1047, 1293
BshNI GGYRCC 1 cut(s) 153
BsiHKAI GWGCWC 3 cut(s) 391, 917, 1184
BsiSI CCGG 2 cut(s) 590, 783
BslFI GGGAC 3 cut(s) 393, 412, 765
BslI CCNNNNNNNGG 1 cut(s) 232
BsmAI GTCTC 1 cut(s) 1482
BsmFI GGGAC 3 cut(s) 393, 412, 765
BsmI GAATGC 1 cut(s) 1486
BsnI GGCC 5 cut(s) 808, 842, 995, 1047, 1293
Bsp1286I GDGCHC 4 cut(s) 391, 917, 1083, 1184
Bsp143I GATC 2 cut(s) 694, 1003
BspACI CCGC 3 cut(s) 38, 306, 1416
BspANI GGCC 5 cut(s) 808, 842, 995, 1047, 1293
BspCNI CTCAG 3 cut(s) 1273, 1351, 1468
BspLI GGNNCC 5 cut(s) 155, 427, 428, 781, 1320
BspMAI CTGCAG 2 cut(s) 369, 616
BspMI ACCTGC 1 cut(s) 943
BspT107I GGYRCC 1 cut(s) 153
BsrBI CCGCTC 1 cut(s) 308
BsrDI GCAATG 3 cut(s) 403, 894, 960
BsrI ACTGG 2 cut(s) 244, 932
BssECI CCNNGG 3 cut(s) 708, 1075, 1076
BssMI GATC 2 cut(s) 694, 1003
BssNAI GTATAC 1 cut(s) 1445
BssT1I CCWWGG 1 cut(s) 708
Bst1107I GTATAC 1 cut(s) 1445
Bst2UI CCWGG 2 cut(s) 743, 1077
Bst4CI ACNGT 2 cut(s) 1351, 1403
Bst6I CTCTTC 1 cut(s) 270
BstAPI GCANNNNNTGC 1 cut(s) 752
BstBAI YACGTR 1 cut(s) 583
BstC8I GCNNGC 5 cut(s) 74, 556, 702, 988, 1045
BstDEI CTNAG 6 cut(s) 837, 887, 1260, 1325, 1359, 1455
BstEII GGTNACC 1 cut(s) 930
BstF5I GGATG 2 cut(s) 310, 792
BstHHI GCGC 1 cut(s) 1043
BstKTI GATC 2 cut(s) 697, 1006
BstMAI GTCTC 1 cut(s) 1482
BstMBI GATC 2 cut(s) 694, 1003
BstMWI GCNNNNNNNGC 4 cut(s) 202, 752, 992, 1040
BstNI CCWGG 2 cut(s) 743, 1077
BstNSI RCATGY 3 cut(s) 13, 76, 602
BstPI GGTNACC 1 cut(s) 930
BstSCI CCNGG 3 cut(s) 741, 781, 1075
BstSFI CTRYAG 3 cut(s) 365, 612, 657
BstSLI GKGCMC 1 cut(s) 391
BstV1I GCAGC 8 cut(s) 53, 260, 351, 379, 787, 911, 925, 1031
BstZ17I GTATAC 1 cut(s) 1445
Bsu36I CCTNAGG 1 cut(s) 1359
BsuRI GGCC 5 cut(s) 808, 842, 995, 1047, 1293
BtgZI GCGATG 1 cut(s) 432
BtsCI GGATG 2 cut(s) 310, 792
BtsIMutI CAGTG 2 cut(s) 822, 1347
BveI ACCTGC 1 cut(s) 943
Cac8I GCNNGC 5 cut(s) 74, 556, 702, 988, 1045
CfoI GCGC 1 cut(s) 1043
Cfr13I GGNCC 4 cut(s) 426, 734, 779, 1292
Csp6I GTAC 2 cut(s) 154, 864
CviAII CATG 7 cut(s) 10, 73, 134, 529, 599, 876, 1049
CviQI GTAC 2 cut(s) 154, 864
DdeI CTNAG 6 cut(s) 837, 887, 1260, 1325, 1359, 1455
DpnI GATC 2 cut(s) 696, 1005
DpnII GATC 2 cut(s) 694, 1003
DraIII CACNNNGTG 1 cut(s) 232
EaeI YGGCCR 1 cut(s) 993
Eam1104I CTCTTC 1 cut(s) 270
EarI CTCTTC 1 cut(s) 270
Ecl136II GAGCTC 1 cut(s) 915
Eco130I CCWWGG 1 cut(s) 708
Eco24I GRGCYC 2 cut(s) 917, 1083
Eco32I GATATC 1 cut(s) 1152
Eco47I GGWCC 3 cut(s) 426, 734, 779
Eco53kI GAGCTC 1 cut(s) 915
Eco57I CTGAAG 1 cut(s) 990
Eco72I CACGTG 1 cut(s) 583
Eco81I CCTNAGG 1 cut(s) 1359
Eco91I GGTNACC 1 cut(s) 930
EcoICRI GAGCTC 1 cut(s) 915
EcoO109I RGGNCCY 1 cut(s) 426
EcoO65I GGTNACC 1 cut(s) 930
EcoRII CCWGG 2 cut(s) 741, 1075
EcoRV GATATC 1 cut(s) 1152
EcoT14I CCWWGG 1 cut(s) 708
EcoT22I ATGCAT 1 cut(s) 223
EcoT38I GRGCYC 2 cut(s) 917, 1083
ErhI CCWWGG 1 cut(s) 708
FaeI CATG 7 cut(s) 13, 76, 137, 532, 602, 879, 1052
FaqI GGGAC 3 cut(s) 393, 412, 765
FatI CATG 7 cut(s) 9, 72, 133, 528, 598, 875, 1048
FbaI TGATCA 2 cut(s) 694, 1003
FblI GTMKAC 1 cut(s) 1444
FokI GGATG 2 cut(s) 297, 779
FriOI GRGCYC 2 cut(s) 917, 1083
FspBI CTAG 4 cut(s) 17, 56, 383, 797
GlaI GCGC 1 cut(s) 1042
GsaI CCCAGC 2 cut(s) 1298, 1434
HaeIII GGCC 5 cut(s) 808, 842, 995, 1047, 1293
HapII CCGG 2 cut(s) 590, 783
HhaI GCGC 1 cut(s) 1043
Hin1II CATG 7 cut(s) 13, 76, 137, 532, 602, 879, 1052
Hin6I GCGC 1 cut(s) 1041
HinP1I GCGC 1 cut(s) 1041
HincII GTYRAC 4 cut(s) 541, 1054, 1384, 1495
HindII GTYRAC 4 cut(s) 541, 1054, 1384, 1495
HinfI GANTC 6 cut(s) 50, 401, 532, 833, 1194, 1244
HpaI GTTAAC 3 cut(s) 1054, 1384, 1495
HpaII CCGG 2 cut(s) 590, 783
HphI GGTGA 6 cut(s) 631, 704, 875, 924, 1153, 1265
Hpy166II GTNNAC 7 cut(s) 389, 541, 976, 1054, 1384, 1445, 1495
Hpy188I TCNGA 2 cut(s) 130, 1263
Hpy188III TCNNGA 4 cut(s) 314, 729, 910, 1001
Hpy8I GTNNAC 7 cut(s) 389, 541, 976, 1054, 1384, 1445, 1495
HpyAV CCTTC 2 cut(s) 437, 1068
HpyCH4III ACNGT 2 cut(s) 1351, 1403
HpyCH4IV ACGT 1 cut(s) 582
HpyF10VI GCNNNNNNNGC 4 cut(s) 202, 752, 992, 1040
HpyF3I CTNAG 6 cut(s) 837, 887, 1260, 1325, 1359, 1455
HpySE526I ACGT 1 cut(s) 582
Hsp92II CATG 7 cut(s) 13, 76, 137, 532, 602, 879, 1052
HspAI GCGC 1 cut(s) 1041
KflI GGGWCCC 1 cut(s) 426
KpnI GGTACC 1 cut(s) 157
Ksp22I TGATCA 2 cut(s) 694, 1003
KspAI GTTAAC 3 cut(s) 1054, 1384, 1495
Kzo9I GATC 2 cut(s) 694, 1003
LmnI GCTCC 3 cut(s) 709, 1324, 1427
Lsp1109I GCAGC 8 cut(s) 53, 260, 351, 379, 787, 911, 925, 1031
LweI GCATC 2 cut(s) 633, 801
MaeI CTAG 4 cut(s) 17, 56, 383, 797
MaeII ACGT 1 cut(s) 582
MaeIII GTNAC 4 cut(s) 12, 51, 605, 930
MalI GATC 2 cut(s) 696, 1005
MbiI CCGCTC 1 cut(s) 308
MboI GATC 2 cut(s) 694, 1003
MboII GAAGA 3 cut(s) 149, 287, 495
MfeI CAATTG 1 cut(s) 329
MhlI GDGCHC 4 cut(s) 391, 917, 1083, 1184
MluCI AATT 9 cut(s) 329, 477, 486, 640, 1132, 1264, 1300, 1313, 1391
MlyI GAGTC 3 cut(s) 59, 1203, 1253
Mph1103I ATGCAT 1 cut(s) 223
MseI TTAA 5 cut(s) 432, 945, 1053, 1383, 1494
MslI CAYNNNNRTG 4 cut(s) 396, 581, 888, 1349
MspI CCGG 2 cut(s) 590, 783
MspR9I CCNGG 3 cut(s) 743, 783, 1077
MunI CAATTG 1 cut(s) 329
Mva1269I GAATGC 1 cut(s) 1486
MvaI CCWGG 2 cut(s) 743, 1077
MwoI GCNNNNNNNGC 4 cut(s) 202, 752, 992, 1040
NciI CCSGG 1 cut(s) 783
NdeII GATC 2 cut(s) 694, 1003
NlaIII CATG 7 cut(s) 13, 76, 137, 532, 602, 879, 1052
NlaIV GGNNCC 5 cut(s) 155, 427, 428, 781, 1320
NmeAIII GCCGAG 1 cut(s) 971
NmuCI GTSAC 3 cut(s) 12, 51, 930
NsiI ATGCAT 1 cut(s) 223
NspI RCATGY 3 cut(s) 13, 76, 602
OliI CACNNNNGTG 1 cut(s) 888
PaeI GCATGC 1 cut(s) 76
PaqCI CACCTGC 1 cut(s) 943
PasI CCCWGGG 1 cut(s) 1076
PciI ACATGT 2 cut(s) 9, 598
PctI GAATGC 1 cut(s) 1486
PfeI GAWTC 3 cut(s) 401, 532, 833
PflMI CCANNNNNTGG 1 cut(s) 232
PleI GAGTC 3 cut(s) 58, 1202, 1252
PmaCI CACGTG 1 cut(s) 583
PmlI CACGTG 1 cut(s) 583
PpsI GAGTC 3 cut(s) 58, 1202, 1252
Ppu21I YACGTR 1 cut(s) 583
PpuMI RGGWCCY 1 cut(s) 426
PscI ACATGT 2 cut(s) 9, 598
Psp124BI GAGCTC 1 cut(s) 917
Psp5II RGGWCCY 1 cut(s) 426
Psp6I CCWGG 2 cut(s) 741, 1075
PspCI CACGTG 1 cut(s) 583
PspEI GGTNACC 1 cut(s) 930
PspFI CCCAGC 2 cut(s) 1294, 1430
PspGI CCWGG 2 cut(s) 741, 1075
PspN4I GGNNCC 5 cut(s) 155, 427, 428, 781, 1320
PspPI GGNCC 4 cut(s) 426, 734, 779, 1292
PspPPI RGGWCCY 1 cut(s) 426
PstI CTGCAG 2 cut(s) 369, 616
RsaI GTAC 2 cut(s) 155, 865
RsaNI GTAC 2 cut(s) 154, 864
RseI CAYNNNNRTG 4 cut(s) 396, 581, 888, 1349
SacI GAGCTC 1 cut(s) 917
SaqAI TTAA 5 cut(s) 432, 945, 1053, 1383, 1494
Sau3AI GATC 2 cut(s) 694, 1003
Sau96I GGNCC 4 cut(s) 426, 734, 779, 1292
SchI GAGTC 3 cut(s) 59, 1203, 1253
ScrFI CCNGG 3 cut(s) 743, 783, 1077
SduI GDGCHC 4 cut(s) 391, 917, 1083, 1184
SfaNI GCATC 2 cut(s) 633, 801
SfcI CTRYAG 3 cut(s) 365, 612, 657
SinI GGWCC 3 cut(s) 426, 734, 779
SmiMI CAYNNNNRTG 4 cut(s) 396, 581, 888, 1349
SmlI CTYRAG 2 cut(s) 197, 908
SmoI CTYRAG 2 cut(s) 197, 908
SpeI ACTAGT 1 cut(s) 16
SphI GCATGC 1 cut(s) 76
Sse9I AATT 9 cut(s) 329, 477, 486, 640, 1132, 1264, 1300, 1313, 1391
SsiI CCGC 3 cut(s) 38, 306, 1416
SspI AATATT 1 cut(s) 759
SspMI CTAG 4 cut(s) 17, 56, 383, 797
SstI GAGCTC 1 cut(s) 917
StyD4I CCNGG 3 cut(s) 741, 781, 1075
StyI CCWWGG 1 cut(s) 708
TaaI ACNGT 2 cut(s) 1351, 1403
TaiI ACGT 1 cut(s) 585
TasI AATT 9 cut(s) 329, 477, 486, 640, 1132, 1264, 1300, 1313, 1391
TauI GCSGC 2 cut(s) 41, 1418
TfiI GAWTC 3 cut(s) 401, 532, 833
Tru1I TTAA 5 cut(s) 432, 945, 1053, 1383, 1494
Tru9I TTAA 5 cut(s) 432, 945, 1053, 1383, 1494
TscAI CASTG 2 cut(s) 822, 1354
TseFI GTSAC 3 cut(s) 12, 51, 930
TseI GCWGC 8 cut(s) 41, 248, 364, 367, 800, 899, 938, 1019
Tsp45I GTSAC 3 cut(s) 12, 51, 930
TspDTI ATGAA 4 cut(s) 45, 150, 393, 714
TspRI CASTG 2 cut(s) 822, 1354
Van91I CCANNNNNTGG 1 cut(s) 232
VneI GTGCAC 1 cut(s) 387
VpaK11BI GGWCC 3 cut(s) 426, 734, 779
XapI RAATTY 1 cut(s) 640
XceI RCATGY 3 cut(s) 13, 76, 602
XmiI GTMKAC 1 cut(s) 1444
XspI CTAG 4 cut(s) 17, 56, 383, 797
Zsp2I ATGCAT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.