MD04G1072600.v1.1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000302
Physical Location & Seq
Reverse (-)
9921351 .. 9921848
498 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1072600.v1.1.491

Sequence Viewer

Length: 498 bp
ATGATTCTCATGAACAAAGAGATTGATGGATTTAGCACCTTGGCTGAAGCTCACGTGCTACCGGCAACACATGTGAGTTATGCTGCAGGGTTGCAAATTAAATTGTATATAAGTTCAACCTCAACACCTAAAGGTACAATCTTGTTCAATGGCACTGTCATCGGAGATGCGCTTGCTCCCAAAGTTGCTTCCTTTTCATCAAGAGGACCAAACATTGCAAGCCCCGGAATTTTGAAACCTGATGTCATTGGACCCGGTGTTAGCATCCTAGCAGCATGGCCTTATAATGTGGACAATGTCACACATCCGAATCCCAAGGCATTATTTAACATCATTTCGGGTACCTCAATGGCCTGTCCTCACCTAAGTGGCATTGCCGCCTTGCTCAAGAGCACCCACCCTGACTGGTCACCAGCTGCTATTAAATCAGCTATCATGACTACCGCTGATGTATTAAACCTATTGGATGTCATTATTAAAAGTAGGGTTAAATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

166

Amino Acids

17.3

Weight (kDa)

9.07

Isoelectric Point (pI)

32.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 39 - 150 3.2e-20 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 285
Acc65I GGTACC 1 cut(s) 341
AccB1I GGYRCC 1 cut(s) 341
AciI CCGC 2 cut(s) 378, 444
AcsI RAATTY 1 cut(s) 228
AcuI CTGAAG 1 cut(s) 66
AcvI CACGTG 1 cut(s) 55
AfaI GTAC 2 cut(s) 136, 343
AflIII ACRYGT 1 cut(s) 70
AgsI TTSAA 3 cut(s) 117, 148, 235
AleI CACNNNNGTG 1 cut(s) 366
AluBI AGCT 3 cut(s) 50, 416, 431
AluI AGCT 3 cut(s) 50, 416, 431
Alw21I GWGCWC 1 cut(s) 395
AoxI GGCC 2 cut(s) 278, 351
ApeKI GCWGC 3 cut(s) 83, 272, 416
ApoI RAATTY 1 cut(s) 228
Asp718I GGTACC 1 cut(s) 341
AspLEI GCGC 1 cut(s) 172
AspS9I GGNCC 2 cut(s) 206, 251
AsuC2I CCSGG 2 cut(s) 225, 255
AsuHPI GGTGA 2 cut(s) 353, 402
AvaII GGWCC 2 cut(s) 206, 251
BanI GGYRCC 1 cut(s) 341
BbrPI CACGTG 1 cut(s) 55
Bbv12I GWGCWC 1 cut(s) 395
BbvI GCAGC 3 cut(s) 70, 284, 403
BccI CCATC 1 cut(s) 20
BcgI CGANNNNNNTGC 2 cut(s) 142, 176
BcnI CCSGG 2 cut(s) 225, 255
BfaI CTAG 1 cut(s) 269
BfmI CTRYAG 1 cut(s) 84
BisI GCNGC 4 cut(s) 84, 273, 378, 417
BlsI GCNGC 4 cut(s) 85, 274, 379, 418
Bme1390I CCNGG 2 cut(s) 225, 255
Bme18I GGWCC 2 cut(s) 206, 251
BmgT120I GGNCC 2 cut(s) 206, 251
BmiI GGNNCC 2 cut(s) 253, 343
BmrFI CCNGG 2 cut(s) 225, 255
BmsI GCATC 2 cut(s) 157, 273
BpuEI CTTGAG 1 cut(s) 371
BpuMI CCSGG 2 cut(s) 225, 255
BsaAI YACGTR 1 cut(s) 55
BsaJI CCNNGG 3 cut(s) 39, 223, 315
Bse118I RCCGGY 1 cut(s) 61
Bse1I ACTGG 1 cut(s) 410
Bse3DI GCAATG 2 cut(s) 213, 372
BseDI CCNNGG 3 cut(s) 39, 223, 315
BseGI GGATG 3 cut(s) 264, 304, 472
BseMI GCAATG 2 cut(s) 213, 372
BseNI ACTGG 1 cut(s) 410
BseXI GCAGC 3 cut(s) 70, 284, 403
BshFI GGCC 2 cut(s) 280, 353
BshNI GGYRCC 1 cut(s) 341
BsiHKAI GWGCWC 1 cut(s) 395
BsiSI CCGG 3 cut(s) 62, 225, 255
BsnI GGCC 2 cut(s) 280, 353
Bsp1286I GDGCHC 1 cut(s) 395
BspACI CCGC 2 cut(s) 378, 444
BspANI GGCC 2 cut(s) 280, 353
BspHI TCATGA 2 cut(s) 9, 435
BspLI GGNNCC 2 cut(s) 253, 343
BspMAI CTGCAG 1 cut(s) 88
BspT107I GGYRCC 1 cut(s) 341
BsrDI GCAATG 2 cut(s) 213, 372
BsrFI RCCGGY 1 cut(s) 61
BsrI ACTGG 1 cut(s) 410
BssAI RCCGGY 1 cut(s) 61
BssECI CCNNGG 3 cut(s) 39, 223, 315
BssT1I CCWWGG 2 cut(s) 39, 315
Bst4CI ACNGT 1 cut(s) 157
BstBAI YACGTR 1 cut(s) 55
BstC8I GCNNGC 2 cut(s) 174, 220
BstDEI CTNAG 1 cut(s) 365
BstEII GGTNACC 1 cut(s) 408
BstF5I GGATG 3 cut(s) 264, 304, 472
BstHHI GCGC 1 cut(s) 172
BstNSI RCATGY 1 cut(s) 74
BstPI GGTNACC 1 cut(s) 408
BstSCI CCNGG 2 cut(s) 223, 253
BstSFI CTRYAG 1 cut(s) 84
BstV1I GCAGC 3 cut(s) 70, 284, 403
BsuRI GGCC 2 cut(s) 280, 353
BtsCI GGATG 3 cut(s) 264, 304, 472
BtsIMutI CAGTG 1 cut(s) 153
Cac8I GCNNGC 2 cut(s) 174, 220
CciI TCATGA 2 cut(s) 9, 435
CfoI GCGC 1 cut(s) 172
Cfr10I RCCGGY 1 cut(s) 61
Cfr13I GGNCC 2 cut(s) 206, 251
Csp6I GTAC 2 cut(s) 135, 342
CviAII CATG 4 cut(s) 10, 71, 276, 436
CviJI RGCY 7 cut(s) 44, 50, 222, 280, 353, 416, 431
CviKI_1 RGCY 7 cut(s) 44, 50, 222, 280, 353, 416, 431
CviQI GTAC 2 cut(s) 135, 342
DdeI CTNAG 1 cut(s) 365
Eco130I CCWWGG 2 cut(s) 39, 315
Eco47I GGWCC 2 cut(s) 206, 251
Eco57I CTGAAG 1 cut(s) 66
Eco72I CACGTG 1 cut(s) 55
Eco91I GGTNACC 1 cut(s) 408
EcoO65I GGTNACC 1 cut(s) 408
EcoT14I CCWWGG 2 cut(s) 39, 315
ErhI CCWWGG 2 cut(s) 39, 315
FaeI CATG 4 cut(s) 13, 74, 279, 439
FaiI YATR 8 cut(s) 11, 72, 81, 108, 110, 277, 285, 437
FatI CATG 4 cut(s) 9, 70, 275, 435
Fnu4HI GCNGC 4 cut(s) 84, 273, 378, 417
FokI GGATG 3 cut(s) 251, 291, 479
Fsp4HI GCNGC 4 cut(s) 84, 273, 378, 417
FspBI CTAG 1 cut(s) 269
GlaI GCGC 1 cut(s) 171
GluI GCNGC 4 cut(s) 84, 273, 378, 417
HaeIII GGCC 2 cut(s) 280, 353
HapII CCGG 3 cut(s) 62, 225, 255
HhaI GCGC 1 cut(s) 172
Hin1II CATG 4 cut(s) 13, 74, 279, 439
Hin6I GCGC 1 cut(s) 170
HinP1I GCGC 1 cut(s) 170
HinfI GANTC 2 cut(s) 4, 310
HpaII CCGG 3 cut(s) 62, 225, 255
HphI GGTGA 2 cut(s) 353, 402
Hpy166II GTNNAC 1 cut(s) 292
Hpy188I TCNGA 2 cut(s) 164, 309
Hpy188III TCNNGA 4 cut(s) 10, 201, 388, 436
Hpy8I GTNNAC 1 cut(s) 292
HpyCH4III ACNGT 1 cut(s) 157
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 3 cut(s) 86, 94, 218
HpyF3I CTNAG 1 cut(s) 365
HpySE526I ACGT 1 cut(s) 54
Hsp92II CATG 4 cut(s) 13, 74, 279, 439
HspAI GCGC 1 cut(s) 170
KpnI GGTACC 1 cut(s) 345
LmnI GCTCC 1 cut(s) 181
LpnPI CCDG 9 cut(s) 72, 75, 238, 252, 268, 367, 391, 414, 426
Lsp1109I GCAGC 3 cut(s) 70, 284, 403
LweI GCATC 2 cut(s) 157, 273
MaeI CTAG 1 cut(s) 269
MaeII ACGT 1 cut(s) 54
MaeIII GTNAC 2 cut(s) 298, 408
MhlI GDGCHC 1 cut(s) 395
MluCI AATT 3 cut(s) 96, 101, 228
MnlI CCTC 4 cut(s) 130, 197, 355, 369
MseI TTAA 6 cut(s) 99, 327, 423, 455, 477, 489
MslI CAYNNNNRTG 1 cut(s) 366
MspA1I CMGCKG 2 cut(s) 416, 446
MspI CCGG 3 cut(s) 62, 225, 255
MspR9I CCNGG 2 cut(s) 225, 255
NciI CCSGG 2 cut(s) 225, 255
NlaIII CATG 4 cut(s) 13, 74, 279, 439
NlaIV GGNNCC 2 cut(s) 253, 343
NmuCI GTSAC 2 cut(s) 298, 408
NspI RCATGY 1 cut(s) 74
OliI CACNNNNGTG 1 cut(s) 366
PagI TCATGA 2 cut(s) 9, 435
PciI ACATGT 1 cut(s) 70
PfeI GAWTC 2 cut(s) 4, 310
PflFI GACNNNGTC 1 cut(s) 296
PkrI GCNGC 4 cut(s) 85, 274, 379, 418
PmaCI CACGTG 1 cut(s) 55
PmlI CACGTG 1 cut(s) 55
Ppu21I YACGTR 1 cut(s) 55
PscI ACATGT 1 cut(s) 70
PsiI TTATAA 1 cut(s) 285
PspCI CACGTG 1 cut(s) 55
PspEI GGTNACC 1 cut(s) 408
PspN4I GGNNCC 2 cut(s) 253, 343
PspPI GGNCC 2 cut(s) 206, 251
PstI CTGCAG 1 cut(s) 88
PsyI GACNNNGTC 1 cut(s) 296
PvuII CAGCTG 1 cut(s) 416
RsaI GTAC 2 cut(s) 136, 343
RsaNI GTAC 2 cut(s) 135, 342
RseI CAYNNNNRTG 1 cut(s) 366
SaqAI TTAA 6 cut(s) 99, 327, 423, 455, 477, 489
SatI GCNGC 4 cut(s) 84, 273, 378, 417
Sau96I GGNCC 2 cut(s) 206, 251
ScrFI CCNGG 2 cut(s) 225, 255
SduI GDGCHC 1 cut(s) 395
SfaNI GCATC 2 cut(s) 157, 273
SfcI CTRYAG 1 cut(s) 84
SinI GGWCC 2 cut(s) 206, 251
SmiMI CAYNNNNRTG 1 cut(s) 366
SmlI CTYRAG 1 cut(s) 386
SmoI CTYRAG 1 cut(s) 386
Sse9I AATT 3 cut(s) 96, 101, 228
SsiI CCGC 2 cut(s) 378, 444
SspI AATATT 1 cut(s) 494
SspMI CTAG 1 cut(s) 269
StyD4I CCNGG 2 cut(s) 223, 253
StyI CCWWGG 2 cut(s) 39, 315
TaaI ACNGT 1 cut(s) 157
TaiI ACGT 1 cut(s) 57
TasI AATT 3 cut(s) 96, 101, 228
TauI GCSGC 1 cut(s) 380
TfiI GAWTC 2 cut(s) 4, 310
Tru1I TTAA 6 cut(s) 99, 327, 423, 455, 477, 489
Tru9I TTAA 6 cut(s) 99, 327, 423, 455, 477, 489
TscAI CASTG 1 cut(s) 160
TseFI GTSAC 2 cut(s) 298, 408
TseI GCWGC 3 cut(s) 83, 272, 416
Tsp45I GTSAC 2 cut(s) 298, 408
TspDTI ATGAA 2 cut(s) 26, 186
TspRI CASTG 1 cut(s) 160
Tth111I GACNNNGTC 1 cut(s) 296
VpaK11BI GGWCC 2 cut(s) 206, 251
XapI RAATTY 1 cut(s) 228
XceI RCATGY 1 cut(s) 74
XspI CTAG 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.