subtilisin-like protease
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 665, 1234 |
| Acc36I | ACCTGC | 2 cut(s) | 665, 1234 |
| Acc65I | GGTACC | 1 cut(s) | 207 |
| AccB1I | GGYRCC | 1 cut(s) | 207 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 286 |
| AccBSI | CCGCTC | 1 cut(s) | 362 |
| AccI | GTMKAC | 1 cut(s) | 1735 |
| AciI | CCGC | 3 cut(s) | 107, 360, 1707 |
| AcoI | YGGCCR | 1 cut(s) | 1284 |
| AcsI | RAATTY | 1 cut(s) | 931 |
| AcuI | CTGAAG | 1 cut(s) | 1281 |
| AcvI | CACGTG | 1 cut(s) | 874 |
| AdeI | CACNNNGTG | 1 cut(s) | 286 |
| AfaI | GTAC | 2 cut(s) | 209, 1156 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 286 |
| AflIII | ACRYGT | 2 cut(s) | 63, 889 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 619 |
| AjnI | CCWGG | 2 cut(s) | 1032, 1366 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 992, 1024 |
| AleI | CACNNNNGTG | 1 cut(s) | 1179 |
| Alw21I | GWGCWC | 3 cut(s) | 682, 1208, 1475 |
| Alw26I | GTCTC | 2 cut(s) | 1612, 1773 |
| Alw44I | GTGCAC | 1 cut(s) | 678 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 80 |
| AoxI | GGCC | 5 cut(s) | 499, 1097, 1284, 1336, 1582 |
| ApaLI | GTGCAC | 1 cut(s) | 678 |
| ApeKI | GCWGC | 8 cut(s) | 95, 302, 418, 421, 1091, 1190, 1229, 1310 |
| ApoI | RAATTY | 1 cut(s) | 931 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 960, 992 |
| Asp718I | GGTACC | 1 cut(s) | 207 |
| AspLEI | GCGC | 2 cut(s) | 446, 1334 |
| AspS9I | GGNCC | 5 cut(s) | 500, 717, 1025, 1070, 1583 |
| AsuC2I | CCSGG | 1 cut(s) | 1074 |
| AsuHPI | GGTGA | 6 cut(s) | 922, 995, 1166, 1215, 1444, 1556 |
| AsuNHI | GCTAGC | 1 cut(s) | 109 |
| AvaII | GGWCC | 3 cut(s) | 717, 1025, 1070 |
| AxyI | CCTNAGG | 1 cut(s) | 1650 |
| BaeGI | GKGCMC | 1 cut(s) | 682 |
| BanI | GGYRCC | 1 cut(s) | 207 |
| BanII | GRGCYC | 2 cut(s) | 1208, 1374 |
| BbrPI | CACGTG | 1 cut(s) | 874 |
| Bbv12I | GWGCWC | 3 cut(s) | 682, 1208, 1475 |
| BbvI | GCAGC | 8 cut(s) | 107, 314, 405, 433, 1078, 1202, 1216, 1322 |
| BccI | CCATC | 5 cut(s) | 328, 341, 491, 1709, 1724 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 155, 189, 961, 995 |
| BciT130I | CCWGG | 2 cut(s) | 1034, 1368 |
| BclI | TGATCA | 2 cut(s) | 985, 1294 |
| BcnI | CCSGG | 1 cut(s) | 1074 |
| BcoDI | GTCTC | 2 cut(s) | 1612, 1773 |
| BfaI | CTAG | 3 cut(s) | 71, 110, 1088 |
| BfmI | CTRYAG | 3 cut(s) | 419, 903, 948 |
| BfuAI | ACCTGC | 2 cut(s) | 665, 1234 |
| Bme1390I | CCNGG | 3 cut(s) | 1034, 1074, 1368 |
| Bme18I | GGWCC | 3 cut(s) | 717, 1025, 1070 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 619 |
| BmgT120I | GGNCC | 5 cut(s) | 500, 717, 1025, 1070, 1583 |
| BmiI | GGNNCC | 6 cut(s) | 105, 209, 718, 719, 1072, 1611 |
| BmrFI | CCNGG | 3 cut(s) | 1034, 1074, 1368 |
| BmsI | GCATC | 4 cut(s) | 15, 856, 924, 1092 |
| BmtI | GCTAGC | 1 cut(s) | 113 |
| BpuEI | CTTGAG | 2 cut(s) | 272, 1184 |
| BpuMI | CCSGG | 1 cut(s) | 1074 |
| BsaAI | YACGTR | 1 cut(s) | 874 |
| BsaBI | GATNNNNATC | 1 cut(s) | 29 |
| BsaJI | CCNNGG | 5 cut(s) | 40, 533, 999, 1366, 1367 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 928, 958 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 286 |
| Bse1I | ACTGG | 4 cut(s) | 158, 298, 493, 1223 |
| Bse21I | CCTNAGG | 1 cut(s) | 1650 |
| Bse3DI | GCAATG | 3 cut(s) | 694, 1185, 1251 |
| Bse8I | GATNNNNATC | 1 cut(s) | 29 |
| BseBI | CCWGG | 2 cut(s) | 1034, 1368 |
| BseDI | CCNNGG | 5 cut(s) | 40, 533, 999, 1366, 1367 |
| BseGI | GGATG | 2 cut(s) | 364, 1083 |
| BseJI | GATNNNNATC | 1 cut(s) | 29 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 286 |
| BseMI | GCAATG | 3 cut(s) | 694, 1185, 1251 |
| BseMII | CTCAG | 4 cut(s) | 1565, 1607, 1641, 1760 |
| BseNI | ACTGG | 4 cut(s) | 158, 298, 493, 1223 |
| BseSI | GKGCMC | 1 cut(s) | 682 |
| BseXI | GCAGC | 8 cut(s) | 107, 314, 405, 433, 1078, 1202, 1216, 1322 |
| BseYI | CCCAGC | 2 cut(s) | 1585, 1721 |
| BshFI | GGCC | 5 cut(s) | 501, 1099, 1286, 1338, 1584 |
| BshNI | GGYRCC | 1 cut(s) | 207 |
| BsiHKAI | GWGCWC | 3 cut(s) | 682, 1208, 1475 |
| BsiSI | CCGG | 2 cut(s) | 881, 1074 |
| BslFI | GGGAC | 3 cut(s) | 447, 703, 1056 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 286 |
| BsmAI | GTCTC | 2 cut(s) | 1612, 1773 |
| BsmFI | GGGAC | 3 cut(s) | 447, 703, 1056 |
| BsmI | GAATGC | 1 cut(s) | 1777 |
| BsnI | GGCC | 5 cut(s) | 501, 1099, 1286, 1338, 1584 |
| Bsp1286I | GDGCHC | 4 cut(s) | 682, 1208, 1374, 1475 |
| Bsp143I | GATC | 3 cut(s) | 30, 985, 1294 |
| Bsp19I | CCATGG | 1 cut(s) | 533 |
| BspACI | CCGC | 3 cut(s) | 107, 360, 1707 |
| BspANI | GGCC | 5 cut(s) | 501, 1099, 1286, 1338, 1584 |
| BspCNI | CTCAG | 4 cut(s) | 1564, 1608, 1642, 1759 |
| BspLI | GGNNCC | 6 cut(s) | 105, 209, 718, 719, 1072, 1611 |
| BspMAI | CTGCAG | 2 cut(s) | 423, 907 |
| BspMI | ACCTGC | 2 cut(s) | 665, 1234 |
| BspOI | GCTAGC | 1 cut(s) | 113 |
| BspT107I | GGYRCC | 1 cut(s) | 207 |
| BsrBI | CCGCTC | 1 cut(s) | 362 |
| BsrDI | GCAATG | 3 cut(s) | 694, 1185, 1251 |
| BsrI | ACTGG | 4 cut(s) | 158, 298, 493, 1223 |
| BssECI | CCNNGG | 5 cut(s) | 40, 533, 999, 1366, 1367 |
| BssMI | GATC | 3 cut(s) | 30, 985, 1294 |
| BssNAI | GTATAC | 1 cut(s) | 1736 |
| BssT1I | CCWWGG | 3 cut(s) | 40, 533, 999 |
| Bst1107I | GTATAC | 1 cut(s) | 1736 |
| Bst2UI | CCWGG | 2 cut(s) | 1034, 1368 |
| Bst4CI | ACNGT | 5 cut(s) | 547, 657, 976, 1642, 1694 |
| Bst6I | CTCTTC | 1 cut(s) | 324 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1043 |
| BstBAI | YACGTR | 1 cut(s) | 874 |
| BstC8I | GCNNGC | 8 cut(s) | 111, 128, 556, 847, 993, 1039, 1279, 1336 |
| BstDEI | CTNAG | 6 cut(s) | 1128, 1178, 1551, 1616, 1650, 1746 |
| BstDSI | CCRYGG | 1 cut(s) | 533 |
| BstEII | GGTNACC | 1 cut(s) | 1221 |
| BstF5I | GGATG | 2 cut(s) | 364, 1083 |
| BstHHI | GCGC | 2 cut(s) | 446, 1334 |
| BstKTI | GATC | 3 cut(s) | 33, 988, 1297 |
| BstMAI | GTCTC | 2 cut(s) | 1612, 1773 |
| BstMBI | GATC | 3 cut(s) | 30, 985, 1294 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 256, 452, 1038, 1043, 1283, 1331 |
| BstNI | CCWGG | 2 cut(s) | 1034, 1368 |
| BstNSI | RCATGY | 3 cut(s) | 67, 130, 893 |
| BstPI | GGTNACC | 1 cut(s) | 1221 |
| BstSCI | CCNGG | 3 cut(s) | 1032, 1072, 1366 |
| BstSFI | CTRYAG | 3 cut(s) | 419, 903, 948 |
| BstSLI | GKGCMC | 1 cut(s) | 682 |
| BstV1I | GCAGC | 8 cut(s) | 107, 314, 405, 433, 1078, 1202, 1216, 1322 |
| BstZ17I | GTATAC | 1 cut(s) | 1736 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1650 |
| BsuRI | GGCC | 5 cut(s) | 501, 1099, 1286, 1338, 1584 |
| BtgI | CCRYGG | 1 cut(s) | 533 |
| BtgZI | GCGATG | 1 cut(s) | 723 |
| BtsCI | GGATG | 2 cut(s) | 364, 1083 |
| BtsIMutI | CAGTG | 2 cut(s) | 972, 1638 |
| BveI | ACCTGC | 2 cut(s) | 665, 1234 |
| Cac8I | GCNNGC | 8 cut(s) | 111, 128, 556, 847, 993, 1039, 1279, 1336 |
| CaiI | CAGNNNCTG | 1 cut(s) | 80 |
| CfoI | GCGC | 2 cut(s) | 446, 1334 |
| Cfr13I | GGNCC | 5 cut(s) | 500, 717, 1025, 1070, 1583 |
| Csp6I | GTAC | 2 cut(s) | 208, 1155 |
| CviAII | CATG | 9 cut(s) | 55, 64, 127, 188, 534, 820, 890, 1167, 1340 |
| CviQI | GTAC | 2 cut(s) | 208, 1155 |
| DdeI | CTNAG | 6 cut(s) | 1128, 1178, 1551, 1616, 1650, 1746 |
| DpnI | GATC | 3 cut(s) | 32, 987, 1296 |
| DpnII | GATC | 3 cut(s) | 30, 985, 1294 |
| DraIII | CACNNNGTG | 1 cut(s) | 286 |
| DriI | GACNNNNNGTC | 1 cut(s) | 619 |
| EaeI | YGGCCR | 1 cut(s) | 1284 |
| Eam1104I | CTCTTC | 1 cut(s) | 324 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 619 |
| EarI | CTCTTC | 1 cut(s) | 324 |
| Ecl136II | GAGCTC | 1 cut(s) | 1206 |
| Eco130I | CCWWGG | 3 cut(s) | 40, 533, 999 |
| Eco24I | GRGCYC | 2 cut(s) | 1208, 1374 |
| Eco32I | GATATC | 1 cut(s) | 1443 |
| Eco47I | GGWCC | 3 cut(s) | 717, 1025, 1070 |
| Eco53kI | GAGCTC | 1 cut(s) | 1206 |
| Eco57I | CTGAAG | 1 cut(s) | 1281 |
| Eco72I | CACGTG | 1 cut(s) | 874 |
| Eco81I | CCTNAGG | 1 cut(s) | 1650 |
| Eco91I | GGTNACC | 1 cut(s) | 1221 |
| EcoICRI | GAGCTC | 1 cut(s) | 1206 |
| EcoO109I | RGGNCCY | 1 cut(s) | 717 |
| EcoO65I | GGTNACC | 1 cut(s) | 1221 |
| EcoRII | CCWGG | 2 cut(s) | 1032, 1366 |
| EcoRV | GATATC | 1 cut(s) | 1443 |
| EcoT14I | CCWWGG | 3 cut(s) | 40, 533, 999 |
| EcoT22I | ATGCAT | 1 cut(s) | 277 |
| EcoT38I | GRGCYC | 2 cut(s) | 1208, 1374 |
| ErhI | CCWWGG | 3 cut(s) | 40, 533, 999 |
| FaeI | CATG | 9 cut(s) | 58, 67, 130, 191, 537, 823, 893, 1170, 1343 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 455, 487 |
| FaqI | GGGAC | 3 cut(s) | 447, 703, 1056 |
| FatI | CATG | 9 cut(s) | 54, 63, 126, 187, 533, 819, 889, 1166, 1339 |
| FbaI | TGATCA | 2 cut(s) | 985, 1294 |
| FblI | GTMKAC | 1 cut(s) | 1735 |
| FokI | GGATG | 2 cut(s) | 351, 1070 |
| FriOI | GRGCYC | 2 cut(s) | 1208, 1374 |
| FspBI | CTAG | 3 cut(s) | 71, 110, 1088 |
| GlaI | GCGC | 2 cut(s) | 445, 1333 |
| GsaI | CCCAGC | 2 cut(s) | 1589, 1725 |
| HaeIII | GGCC | 5 cut(s) | 501, 1099, 1286, 1338, 1584 |
| HapII | CCGG | 2 cut(s) | 881, 1074 |
| HhaI | GCGC | 2 cut(s) | 446, 1334 |
| Hin1II | CATG | 9 cut(s) | 58, 67, 130, 191, 537, 823, 893, 1170, 1343 |
| Hin6I | GCGC | 2 cut(s) | 444, 1332 |
| HinP1I | GCGC | 2 cut(s) | 444, 1332 |
| HincII | GTYRAC | 3 cut(s) | 832, 1345, 1675 |
| HindII | GTYRAC | 3 cut(s) | 832, 1345, 1675 |
| HindIII | AAGCTT | 1 cut(s) | 590 |
| HinfI | GANTC | 9 cut(s) | 435, 468, 538, 620, 692, 823, 1124, 1485, 1535 |
| HpaI | GTTAAC | 3 cut(s) | 832, 1345, 1675 |
| HpaII | CCGG | 2 cut(s) | 881, 1074 |
| HphI | GGTGA | 6 cut(s) | 922, 995, 1166, 1215, 1444, 1556 |
| Hpy166II | GTNNAC | 7 cut(s) | 680, 832, 1267, 1345, 1675, 1736, 1786 |
| Hpy188I | TCNGA | 3 cut(s) | 184, 462, 1554 |
| Hpy188III | TCNNGA | 4 cut(s) | 368, 1020, 1201, 1292 |
| Hpy8I | GTNNAC | 7 cut(s) | 680, 832, 1267, 1345, 1675, 1736, 1786 |
| Hpy99I | CGWCG | 1 cut(s) | 443 |
| HpyAV | CCTTC | 4 cut(s) | 313, 457, 728, 1359 |
| HpyCH4III | ACNGT | 5 cut(s) | 547, 657, 976, 1642, 1694 |
| HpyCH4IV | ACGT | 1 cut(s) | 873 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 256, 452, 1038, 1043, 1283, 1331 |
| HpyF3I | CTNAG | 6 cut(s) | 1128, 1178, 1551, 1616, 1650, 1746 |
| HpySE526I | ACGT | 1 cut(s) | 873 |
| Hsp92II | CATG | 9 cut(s) | 58, 67, 130, 191, 537, 823, 893, 1170, 1343 |
| HspAI | GCGC | 2 cut(s) | 444, 1332 |
| KflI | GGGWCCC | 1 cut(s) | 717 |
| KpnI | GGTACC | 1 cut(s) | 211 |
| Ksp22I | TGATCA | 2 cut(s) | 985, 1294 |
| KspAI | GTTAAC | 3 cut(s) | 832, 1345, 1675 |
| Kzo9I | GATC | 3 cut(s) | 30, 985, 1294 |
| LmnI | GCTCC | 7 cut(s) | 103, 452, 535, 551, 1000, 1615, 1718 |
| Lsp1109I | GCAGC | 8 cut(s) | 107, 314, 405, 433, 1078, 1202, 1216, 1322 |
| LweI | GCATC | 4 cut(s) | 15, 856, 924, 1092 |
| MaeI | CTAG | 3 cut(s) | 71, 110, 1088 |
| MaeII | ACGT | 1 cut(s) | 873 |
| MaeIII | GTNAC | 3 cut(s) | 66, 896, 1221 |
| MalI | GATC | 3 cut(s) | 32, 987, 1296 |
| MbiI | CCGCTC | 1 cut(s) | 362 |
| MboI | GATC | 3 cut(s) | 30, 985, 1294 |
| MboII | GAAGA | 3 cut(s) | 203, 341, 786 |
| MfeI | CAATTG | 1 cut(s) | 383 |
| MhlI | GDGCHC | 4 cut(s) | 682, 1208, 1374, 1475 |
| MlyI | GAGTC | 4 cut(s) | 429, 629, 1494, 1544 |
| MmeI | TCCRAC | 1 cut(s) | 529 |
| Mph1103I | ATGCAT | 1 cut(s) | 277 |
| MseI | TTAA | 5 cut(s) | 723, 831, 1236, 1344, 1674 |
| MslI | CAYNNNNRTG | 3 cut(s) | 687, 1179, 1640 |
| MspA1I | CMGCKG | 1 cut(s) | 480 |
| MspI | CCGG | 2 cut(s) | 881, 1074 |
| MspR9I | CCNGG | 3 cut(s) | 1034, 1074, 1368 |
| MunI | CAATTG | 1 cut(s) | 383 |
| Mva1269I | GAATGC | 1 cut(s) | 1777 |
| MvaI | CCWGG | 2 cut(s) | 1034, 1368 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 256, 452, 1038, 1043, 1283, 1331 |
| NciI | CCSGG | 1 cut(s) | 1074 |
| NcoI | CCATGG | 1 cut(s) | 533 |
| NdeII | GATC | 3 cut(s) | 30, 985, 1294 |
| NheI | GCTAGC | 1 cut(s) | 109 |
| NlaIII | CATG | 9 cut(s) | 58, 67, 130, 191, 537, 823, 893, 1170, 1343 |
| NlaIV | GGNNCC | 6 cut(s) | 105, 209, 718, 719, 1072, 1611 |
| NmeAIII | GCCGAG | 1 cut(s) | 1262 |
| NmuCI | GTSAC | 2 cut(s) | 66, 1221 |
| NsiI | ATGCAT | 1 cut(s) | 277 |
| NspI | RCATGY | 3 cut(s) | 67, 130, 893 |
| OliI | CACNNNNGTG | 1 cut(s) | 1179 |
| PaeI | GCATGC | 1 cut(s) | 130 |
| PaqCI | CACCTGC | 2 cut(s) | 665, 1234 |
| PasI | CCCWGGG | 1 cut(s) | 1367 |
| PciI | ACATGT | 2 cut(s) | 63, 889 |
| PctI | GAATGC | 1 cut(s) | 1777 |
| PfeI | GAWTC | 5 cut(s) | 468, 538, 692, 823, 1124 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 286 |
| PleI | GAGTC | 4 cut(s) | 429, 628, 1493, 1543 |
| PmaCI | CACGTG | 1 cut(s) | 874 |
| PmlI | CACGTG | 1 cut(s) | 874 |
| PpsI | GAGTC | 4 cut(s) | 429, 628, 1493, 1543 |
| Ppu21I | YACGTR | 1 cut(s) | 874 |
| PpuMI | RGGWCCY | 1 cut(s) | 717 |
| PscI | ACATGT | 2 cut(s) | 63, 889 |
| Psp124BI | GAGCTC | 1 cut(s) | 1208 |
| Psp5II | RGGWCCY | 1 cut(s) | 717 |
| Psp6I | CCWGG | 2 cut(s) | 1032, 1366 |
| PspCI | CACGTG | 1 cut(s) | 874 |
| PspEI | GGTNACC | 1 cut(s) | 1221 |
| PspFI | CCCAGC | 2 cut(s) | 1585, 1721 |
| PspGI | CCWGG | 2 cut(s) | 1032, 1366 |
| PspN4I | GGNNCC | 6 cut(s) | 105, 209, 718, 719, 1072, 1611 |
| PspPI | GGNCC | 5 cut(s) | 500, 717, 1025, 1070, 1583 |
| PspPPI | RGGWCCY | 1 cut(s) | 717 |
| PstI | CTGCAG | 2 cut(s) | 423, 907 |
| PstNI | CAGNNNCTG | 1 cut(s) | 80 |
| PvuII | CAGCTG | 1 cut(s) | 480 |
| RsaI | GTAC | 2 cut(s) | 209, 1156 |
| RsaNI | GTAC | 2 cut(s) | 208, 1155 |
| RseI | CAYNNNNRTG | 3 cut(s) | 687, 1179, 1640 |
| SacI | GAGCTC | 1 cut(s) | 1208 |
| SaqAI | TTAA | 5 cut(s) | 723, 831, 1236, 1344, 1674 |
| Sau3AI | GATC | 3 cut(s) | 30, 985, 1294 |
| Sau96I | GGNCC | 5 cut(s) | 500, 717, 1025, 1070, 1583 |
| SchI | GAGTC | 4 cut(s) | 429, 629, 1494, 1544 |
| ScrFI | CCNGG | 3 cut(s) | 1034, 1074, 1368 |
| SduI | GDGCHC | 4 cut(s) | 682, 1208, 1374, 1475 |
| SfaNI | GCATC | 4 cut(s) | 15, 856, 924, 1092 |
| SfcI | CTRYAG | 3 cut(s) | 419, 903, 948 |
| SinI | GGWCC | 3 cut(s) | 717, 1025, 1070 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 687, 1179, 1640 |
| SmlI | CTYRAG | 2 cut(s) | 251, 1199 |
| SmoI | CTYRAG | 2 cut(s) | 251, 1199 |
| SphI | GCATGC | 1 cut(s) | 130 |
| SsiI | CCGC | 3 cut(s) | 107, 360, 1707 |
| SspMI | CTAG | 3 cut(s) | 71, 110, 1088 |
| SstI | GAGCTC | 1 cut(s) | 1208 |
| StyD4I | CCNGG | 3 cut(s) | 1032, 1072, 1366 |
| StyI | CCWWGG | 3 cut(s) | 40, 533, 999 |
| TaaI | ACNGT | 5 cut(s) | 547, 657, 976, 1642, 1694 |
| TaiI | ACGT | 1 cut(s) | 876 |
| TauI | GCSGC | 2 cut(s) | 109, 1709 |
| TfiI | GAWTC | 5 cut(s) | 468, 538, 692, 823, 1124 |
| Tru1I | TTAA | 5 cut(s) | 723, 831, 1236, 1344, 1674 |
| Tru9I | TTAA | 5 cut(s) | 723, 831, 1236, 1344, 1674 |
| TscAI | CASTG | 2 cut(s) | 979, 1645 |
| TseFI | GTSAC | 2 cut(s) | 66, 1221 |
| TseI | GCWGC | 8 cut(s) | 95, 302, 418, 421, 1091, 1190, 1229, 1310 |
| Tsp45I | GTSAC | 2 cut(s) | 66, 1221 |
| TspDTI | ATGAA | 6 cut(s) | 43, 99, 204, 540, 684, 1005 |
| TspRI | CASTG | 2 cut(s) | 979, 1645 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 286 |
| VneI | GTGCAC | 1 cut(s) | 678 |
| VpaK11BI | GGWCC | 3 cut(s) | 717, 1025, 1070 |
| XapI | RAATTY | 1 cut(s) | 931 |
| XceI | RCATGY | 3 cut(s) | 67, 130, 893 |
| XmiI | GTMKAC | 1 cut(s) | 1735 |
| XspI | CTAG | 3 cut(s) | 71, 110, 1088 |
| Zsp2I | ATGCAT | 1 cut(s) | 277 |