Prupe.5G085000_v2.0.a1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000302
Physical Location & Seq
Reverse (-)
9715799 .. 9718090
2292 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G085000.1

Sequence Viewer

Length: 2292 bp
ATGGAGAGCAAAAAGTGTGTGGTGTTGCAAATGCAGATTGTATATCTTCTTAGCTTGCTTTTCATGTCTTCTTTATCACTGGCTGCAGATGAGGAGCAAAATGGCATGCAAACTTATATAGTTTGGGTAGAAAAGCCGGTGGCTCAGGGATCTTCTGCACAATCTCATGAAGATTTGGAGAGTTGGTACCAGACATTTTTGCCAGAAAGTACTATTGCAACCTCAAACCAGCTGTCGAAGCCAAGAATCGTTCATGCATACCACAATGTGGCGACCGGGTTTGCAGCAAAACTGACACCAGAGGAAGTGAAAGCAATGGAAAAGAAACCAGGGTTTGTGTCAGCTCATCCAGAGGCAATCCTGCCTTTGCACACAACTCACAGCCCCAACTTCTTGGGTTTGAACCAAGGGTTGGGATTTTGGAAAGGATCAAACTATGGTAAAGGTGTGATCATTGGAGTTTTGGATACTGGGGTTTCACCAGATCATCCCTCATTTAGTGACGCAGGAGTGCCTCCTCCACCAGCTAAATGGAAAGGCAAATGTGAGTTCAATGGCAGAGTGTGCAACAACAAGCTTATTGGTGCAAGAAACTTCAATGGCATAAGCACAGGGCAACCTGCAGGAGACCCTCCATTTGACCAAGAAGGCCACGGGACCCACACGTCCAGCACAGCTGCCGGGAACTTTGTCAAAGGCGCCGCGGTGTTTGGAATGGTCAAGGGCACAGCGGTTGGCATGGCGCCTTATGCTCACTTGGCAATCTACAGAGTGTGCTCTGTGGCGGGTTGTGCTGAAGGTGACATTTTGGCTGCAATGGATGCTGCGGTTGATGATGGGGTGGACGTACTTTCGCTCTCCCTTGGCGGTTTCTCGCGTCCTTTCTATTCAGATGGGGTTGCAGTTGGTGCATTTGGAGCAATTCAAAAGGGAATTTTTGTGAGCTGTTCAGCTGGAAACTCTGGTCCTTCCTATTCTACTTCATCAAATGAGGCCCCATGGATTCTCACCGTCGGAGCAAGCACGATTGACAGAAACATCAGAGCAACAGCACGGCTCGGGAACAAGAAAGAGTACAATGGCGAATCTCTGTTCCAGCCTAACAATTTTAACTCAAAAATTATGCTACCTCTTGTTGACGCAGGCTCACTTGGAAACCAAACATCAGCTTTTTGCGATCCAGGAACTCTTAAAAACGTTAAAGGGAAAATAGTTTTATGTGAGAGAGGTGGAGCAGGTGGAAGAATTGACAAAGGAGCAGAAGTAAAAAGAGCTGGTGGAGCTGCCATGATTCTCATGAACCAAAGGATTGATGGCTTTAGCACATTAGCTGACCCTCATGTGCTACCCACAGCACATGTGAGTTATGATGCAGGGTTGAAAATCAAGTCCTATTTAAAGTCAACCACAAAGCCTACAGCCACAATTTTGTTCAAAGGCACTGTTATTGGAGATAAACATGCTCCGAGTGTCGCTTCCTTTTCATCAAGAGGACCGAGCACTGCAAGCCCCGGGATTTTGAAACCGGACATCACTGGTCCCGGTGTTAGCATCCTAGCAACATGGCCTGTTTCAGTGGACAATGCCACAAAATCTAAGGCAACATTTAACATAATTTCAGGTACCTCAATGGCATGCCCTCACCTAAGTGGCATTGCAGCCTTGCTCAAGGGCTCACACCCTGACTGGTCACCCGCTGCTATCAAATCAGCAATCATGACAACCGCCGATGTACATAACCTCGGAGGTAAGTCCATTGTTGATCAAGCACTCAAGGCAGCAGACCTTTTTGCAATAGGTGCAGGCCATGTTAACCCTTCAAAAGCAAATGATCCAGGGCTAGTCTACGACACGCAACCGAATAATTACATCCAATACCTGTGTGGCTTGAATTACACAGACAAACAGATACAGATCATAACCCAACAAACAGTGGATTGCTCCAAAATTGGAGCCATACCAGAAGCACAGCTAAACTACCCTTCATTTTCTATTATAATTGGCTCCAATAAAAAGACTAGGTCTCAGCTTTATACAAGGACAGTGAAGAATGTTGGGGAGGCAAATTCAACTTACAAGTTGGATATTTTAGCACCACACAAAGTGGATGTGAATGTGAGCCCTGAGGTGCTTAAATTCACAAAGGTTAAGCAGACTATTACATATCGTGTGAAGTTTGTTGCACAAGAGGGTGCTGGGAAGGATGGTGTTTTGTTTGGCAAGGGATATTTGAGGTGGGTTTCTGATAACCACAATGTTGCTAGCCCAATAGTTGTGATCTTTGGGTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

764

Amino Acids

80.58

Weight (kDa)

8.89

Isoelectric Point (pI)

24.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1997
AarI CACCTGC 1 cut(s) 1226
AasI GACNNNNNNGTC 1 cut(s) 664
Acc36I ACCTGC 2 cut(s) 628, 1226
Acc65I GGTACC 2 cut(s) 186, 1622
AccB1I GGYRCC 4 cut(s) 186, 698, 742, 1622
AccB7I CCANNNNNTGG 2 cut(s) 268, 412
AccI GTMKAC 1 cut(s) 1845
AccII CGCG 2 cut(s) 704, 877
AciI CCGC 8 cut(s) 702, 704, 731, 785, 827, 867, 1695, 1725
AclI AACGTT 1 cut(s) 1197
AclWI GGATC 4 cut(s) 157, 436, 1172, 1826
AcsI RAATTY 3 cut(s) 933, 2065, 2135
AcuI CTGAAG 1 cut(s) 816
AcyI GRCGYC 2 cut(s) 699, 743
AdeI CACNNNGTG 2 cut(s) 268, 2104
AfaI GTAC 6 cut(s) 188, 211, 849, 1076, 1624, 1734
AfiI CCNNNNNNNGG 2 cut(s) 268, 412
AflIII ACRYGT 2 cut(s) 663, 1357
AjiI CACGTC 1 cut(s) 666
AjnI CCWGG 3 cut(s) 328, 1180, 1834
AjuI GAANNNNNNNTTGG 2 cut(s) 395, 427
AleI CACNNNNGTG 1 cut(s) 1647
Alw21I GWGCWC 2 cut(s) 779, 1502
Alw26I GTCTC 2 cut(s) 621, 2028
AlwI GGATC 4 cut(s) 157, 436, 1172, 1826
Ama87I CYCGRG 2 cut(s) 1058, 1511
AoxI GGCC 4 cut(s) 649, 993, 1565, 1804
ApeKI GCWGC 9 cut(s) 83, 284, 677, 812, 824, 1283, 1658, 1697, 1778
ApoI RAATTY 3 cut(s) 933, 2065, 2135
ArsI GACNNNNNNTTYG 4 cut(s) 218, 250, 620, 652
Asp718I GGTACC 2 cut(s) 186, 1622
AspLEI GCGC 2 cut(s) 701, 745
AspS9I GGNCC 5 cut(s) 657, 965, 994, 1493, 1538
AsuC2I CCSGG 5 cut(s) 277, 682, 1512, 1513, 1542
AsuHPI GGTGA 5 cut(s) 471, 812, 1000, 1634, 1683
AsuNHI GCTAGC 1 cut(s) 2261
AvaI CYCGRG 2 cut(s) 1058, 1511
AvaII GGWCC 4 cut(s) 657, 965, 1493, 1538
AxyI CCTNAGG 1 cut(s) 2124
BaeGI GKGCMC 1 cut(s) 728
BaeI ACNNNNGTAYC 2 cut(s) 459, 492
BanI GGYRCC 4 cut(s) 186, 698, 742, 1622
BanII GRGCYC 2 cut(s) 1676, 2123
BbsI GAAGAC 1 cut(s) 60
Bbv12I GWGCWC 2 cut(s) 779, 1502
BbvI GCAGC 9 cut(s) 70, 296, 664, 799, 811, 1270, 1670, 1684, 1790
BccI CCATC 4 cut(s) 830, 887, 1307, 2198
BceAI ACGGC 1 cut(s) 1070
BciT130I CCWGG 3 cut(s) 330, 1182, 1836
BciVI GTATCC 1 cut(s) 460
BclI TGATCA 2 cut(s) 450, 1762
BcnI CCSGG 5 cut(s) 277, 682, 1512, 1513, 1542
BcoDI GTCTC 2 cut(s) 621, 2028
BfaI CTAG 4 cut(s) 1556, 1841, 2019, 2262
BfmI CTRYAG 4 cut(s) 84, 621, 766, 1416
BfoI RGCGCY 2 cut(s) 702, 746
BfuAI ACCTGC 2 cut(s) 628, 1226
BfuI GTATCC 1 cut(s) 460
BmcAI AGTACT 1 cut(s) 211
Bme1390I CCNGG 8 cut(s) 277, 330, 682, 1182, 1512, 1513, 1542, 1836
Bme18I GGWCC 4 cut(s) 657, 965, 1493, 1538
BmeT110I CYCGRG 2 cut(s) 1058, 1511
BmgBI CACGTC 1 cut(s) 666
BmgT120I GGNCC 5 cut(s) 657, 965, 994, 1493, 1538
BmrFI CCNGG 8 cut(s) 277, 330, 682, 1182, 1512, 1513, 1542, 1836
BmrI ACTGGG 1 cut(s) 480
BmsI GCATC 3 cut(s) 811, 1360, 1560
BmtI GCTAGC 1 cut(s) 2265
BmuI ACTGGG 1 cut(s) 480
BpiI GAAGAC 1 cut(s) 60
Bpu10I CCTNAGC 1 cut(s) 144
BpuEI CTTGAG 2 cut(s) 1652, 1757
BpuMI CCSGG 5 cut(s) 277, 682, 1512, 1513, 1542
BsaBI GATNNNNATC 1 cut(s) 1913
BsaHI GRCGYC 2 cut(s) 699, 743
BsaI GGTCTC 2 cut(s) 621, 2028
BsaWI WCCGGW 1 cut(s) 1525
BsaXI ACNNNNNCTCC 4 cut(s) 170, 200, 1248, 1278
Bsc4I CCNNNNNNNGG 2 cut(s) 268, 412
Bse118I RCCGGY 1 cut(s) 136
Bse1I ACTGG 4 cut(s) 84, 475, 1540, 1691
Bse21I CCTNAGG 1 cut(s) 2124
Bse3DI GCAATG 3 cut(s) 321, 822, 1653
Bse8I GATNNNNATC 1 cut(s) 1913
BseBI CCWGG 3 cut(s) 330, 1182, 1836
BseGI GGATG 7 cut(s) 346, 487, 826, 1551, 1869, 2113, 2209
BseJI GATNNNNATC 1 cut(s) 1913
BseLI CCNNNNNNNGG 2 cut(s) 268, 412
BseMI GCAATG 3 cut(s) 321, 822, 1653
BseMII CTCAG 3 cut(s) 158, 2039, 2115
BseNI ACTGG 4 cut(s) 84, 475, 1540, 1691
BseRI GAGGAG 2 cut(s) 107, 507
BseSI GKGCMC 1 cut(s) 728
BseXI GCAGC 9 cut(s) 70, 296, 664, 799, 811, 1270, 1670, 1684, 1790
BseYI CCCAGC 1 cut(s) 2195
BsgI GTGCAG 2 cut(s) 141, 1821
Bsh1236I CGCG 2 cut(s) 704, 877
Bsh1285I CGRYCG 1 cut(s) 276
BshFI GGCC 4 cut(s) 651, 995, 1567, 1806
BshNI GGYRCC 4 cut(s) 186, 698, 742, 1622
BsiEI CGRYCG 1 cut(s) 276
BsiHKAI GWGCWC 2 cut(s) 779, 1502
BsiHKCI CYCGRG 2 cut(s) 1058, 1511
BsiSI CCGG 6 cut(s) 137, 276, 681, 1512, 1526, 1542
BslFI GGGAC 2 cut(s) 670, 1524
BslI CCNNNNNNNGG 2 cut(s) 268, 412
BsmAI GTCTC 2 cut(s) 621, 2028
BsmFI GGGAC 2 cut(s) 670, 1524
BsnI GGCC 4 cut(s) 651, 995, 1567, 1806
Bso31I GGTCTC 2 cut(s) 621, 2028
BsoBI CYCGRG 2 cut(s) 1058, 1511
Bsp1286I GDGCHC 5 cut(s) 728, 779, 1502, 1676, 2123
Bsp1407I TGTACA 1 cut(s) 1732
Bsp143I GATC 9 cut(s) 149, 428, 450, 484, 1177, 1762, 1831, 1914, 2277
Bsp19I CCATGG 1 cut(s) 998
BspACI CCGC 8 cut(s) 702, 704, 731, 785, 827, 867, 1695, 1725
BspANI GGCC 4 cut(s) 651, 995, 1567, 1806
BspCNI CTCAG 3 cut(s) 157, 2038, 2116
BspFNI CGCG 2 cut(s) 704, 877
BspHI TCATGA 3 cut(s) 166, 1296, 1716
BspMAI CTGCAG 2 cut(s) 88, 625
BspMI ACCTGC 2 cut(s) 628, 1226
BspOI GCTAGC 1 cut(s) 2265
BspPI GGATC 4 cut(s) 157, 436, 1172, 1826
BspT107I GGYRCC 4 cut(s) 186, 698, 742, 1622
BspTNI GGTCTC 2 cut(s) 621, 2028
BsrDI GCAATG 3 cut(s) 321, 822, 1653
BsrFI RCCGGY 1 cut(s) 136
BsrGI TGTACA 1 cut(s) 1732
BsrI ACTGG 4 cut(s) 84, 475, 1540, 1691
BssAI RCCGGY 1 cut(s) 136
BssMI GATC 9 cut(s) 149, 428, 450, 484, 1177, 1762, 1831, 1914, 2277
BssNI GRCGYC 2 cut(s) 699, 743
BssT1I CCWWGG 3 cut(s) 406, 862, 998
Bst2UI CCWGG 3 cut(s) 330, 1182, 1836
Bst4CI ACNGT 4 cut(s) 1012, 1444, 1933, 2044
BstACI GRCGYC 2 cut(s) 699, 743
BstAPI GCANNNNNTGC 4 cut(s) 564, 821, 908, 1799
BstAUI TGTACA 1 cut(s) 1732
BstC8I GCNNGC 8 cut(s) 56, 107, 1021, 1144, 1507, 1636, 1804, 2263
BstDEI CTNAG 7 cut(s) 50, 144, 1596, 1646, 2025, 2124, 2289
BstDSI CCRYGG 3 cut(s) 652, 702, 998
BstEII GGTNACC 1 cut(s) 1689
BstF5I GGATG 7 cut(s) 346, 487, 826, 1551, 1869, 2113, 2209
BstFNI CGCG 2 cut(s) 704, 877
BstH2I RGCGCY 2 cut(s) 702, 746
BstHHI GCGC 2 cut(s) 701, 745
BstKTI GATC 9 cut(s) 152, 431, 453, 487, 1180, 1765, 1834, 1917, 2280
BstMAI GTCTC 2 cut(s) 621, 2028
BstMBI GATC 9 cut(s) 149, 428, 450, 484, 1177, 1762, 1831, 1914, 2277
BstMCI CGRYCG 1 cut(s) 276
BstNI CCWGG 3 cut(s) 330, 1182, 1836
BstNSI RCATGY 4 cut(s) 109, 1361, 1463, 1638
BstPI GGTNACC 1 cut(s) 1689
BstSCI CCNGG 8 cut(s) 275, 328, 680, 1180, 1510, 1511, 1540, 1834
BstSFI CTRYAG 4 cut(s) 84, 621, 766, 1416
BstSLI GKGCMC 1 cut(s) 728
BstUI CGCG 2 cut(s) 704, 877
BstV1I GCAGC 9 cut(s) 70, 296, 664, 799, 811, 1270, 1670, 1684, 1790
BstV2I GAAGAC 1 cut(s) 60
BstX2I RGATCY 1 cut(s) 149
BstXI CCANNNNNNTGG 2 cut(s) 394, 531
BstYI RGATCY 1 cut(s) 149
Bsu36I CCTNAGG 1 cut(s) 2124
BsuI GTATCC 1 cut(s) 460
BsuRI GGCC 4 cut(s) 651, 995, 1567, 1806
BtgI CCRYGG 3 cut(s) 652, 702, 998
BtrI CACGTC 1 cut(s) 666
BtsCI GGATG 7 cut(s) 346, 487, 826, 1551, 1869, 2113, 2209
BtsI GCAGTG 1 cut(s) 1500
BtsIMutI CAGTG 7 cut(s) 77, 1440, 1500, 1533, 1581, 1938, 2049
BveI ACCTGC 2 cut(s) 628, 1226
Cac8I GCNNGC 8 cut(s) 56, 107, 1021, 1144, 1507, 1636, 1804, 2263
CciI TCATGA 3 cut(s) 166, 1296, 1716
CfoI GCGC 2 cut(s) 701, 745
Cfr10I RCCGGY 1 cut(s) 136
Cfr13I GGNCC 5 cut(s) 657, 965, 994, 1493, 1538
Cfr42I CCGCGG 1 cut(s) 705
Cfr9I CCCGGG 1 cut(s) 1511
CseI GACGC 3 cut(s) 512, 866, 1148
Csp6I GTAC 6 cut(s) 187, 210, 848, 1075, 1623, 1733
CviQI GTAC 6 cut(s) 187, 210, 848, 1075, 1623, 1733
DdeI CTNAG 7 cut(s) 50, 144, 1596, 1646, 2025, 2124, 2289
DinI GGCGCC 2 cut(s) 700, 744
DpnI GATC 9 cut(s) 151, 430, 452, 486, 1179, 1764, 1833, 1916, 2279
DpnII GATC 9 cut(s) 149, 428, 450, 484, 1177, 1762, 1831, 1914, 2277
DraI TTTAAA 1 cut(s) 1398
DraIII CACNNNGTG 2 cut(s) 268, 2104
DrdI GACNNNNNNGTC 1 cut(s) 664
DseDI GACNNNNNNGTC 1 cut(s) 664
Eco130I CCWWGG 3 cut(s) 406, 862, 998
Eco24I GRGCYC 2 cut(s) 1676, 2123
Eco31I GGTCTC 2 cut(s) 621, 2028
Eco47I GGWCC 4 cut(s) 657, 965, 1493, 1538
Eco57I CTGAAG 1 cut(s) 816
Eco81I CCTNAGG 1 cut(s) 2124
Eco88I CYCGRG 2 cut(s) 1058, 1511
Eco91I GGTNACC 1 cut(s) 1689
EcoO109I RGGNCCY 2 cut(s) 657, 994
EcoO65I GGTNACC 1 cut(s) 1689
EcoRII CCWGG 3 cut(s) 328, 1180, 1834
EcoT14I CCWWGG 3 cut(s) 406, 862, 998
EcoT22I ATGCAT 1 cut(s) 259
EcoT38I GRGCYC 2 cut(s) 1676, 2123
EgeI GGCGCC 2 cut(s) 700, 744
EheI GGCGCC 2 cut(s) 700, 744
ErhI CCWWGG 3 cut(s) 406, 862, 998
FaqI GGGAC 2 cut(s) 670, 1524
FauI CCCGC 2 cut(s) 778, 1702
FbaI TGATCA 2 cut(s) 450, 1762
FblI GTMKAC 1 cut(s) 1845
FokI GGATG 7 cut(s) 333, 474, 833, 1538, 1856, 2120, 2216
FriOI GRGCYC 2 cut(s) 1676, 2123
FspBI CTAG 4 cut(s) 1556, 1841, 2019, 2262
GlaI GCGC 2 cut(s) 700, 744
GsaI CCCAGC 1 cut(s) 2199
HaeII RGCGCY 2 cut(s) 702, 746
HaeIII GGCC 4 cut(s) 651, 995, 1567, 1806
HapII CCGG 6 cut(s) 137, 276, 681, 1512, 1526, 1542
HgaI GACGC 3 cut(s) 512, 866, 1148
HhaI GCGC 2 cut(s) 701, 745
Hin1I GRCGYC 2 cut(s) 699, 743
Hin6I GCGC 2 cut(s) 699, 743
HinP1I GCGC 2 cut(s) 699, 743
HincII GTYRAC 3 cut(s) 1138, 1404, 1813
HindII GTYRAC 3 cut(s) 1138, 1404, 1813
HindIII AAGCTT 1 cut(s) 575
HinfI GANTC 4 cut(s) 246, 1003, 1085, 1291
HpaI GTTAAC 1 cut(s) 1813
HpaII CCGG 6 cut(s) 137, 276, 681, 1512, 1526, 1542
HphI GGTGA 5 cut(s) 471, 812, 1000, 1634, 1683
Hpy166II GTNNAC 6 cut(s) 844, 1138, 1404, 1579, 1813, 1846
Hpy188I TCNGA 6 cut(s) 892, 1016, 1043, 1467, 1745, 2245
Hpy188III TCNNGA 6 cut(s) 167, 350, 1060, 1297, 1488, 1717
Hpy8I GTNNAC 6 cut(s) 844, 1138, 1404, 1579, 1813, 1846
Hpy99I CGWCG 1 cut(s) 1016
HpyAV CCTTC 6 cut(s) 641, 791, 978, 1827, 1992, 2194
HpyCH4III ACNGT 4 cut(s) 1012, 1444, 1933, 2044
HpyCH4IV ACGT 3 cut(s) 665, 846, 1197
HpyF3I CTNAG 7 cut(s) 50, 144, 1596, 1646, 2025, 2124, 2289
HpySE526I ACGT 3 cut(s) 665, 846, 1197
Hsp92I GRCGYC 2 cut(s) 699, 743
HspAI GCGC 2 cut(s) 699, 743
KasI GGCGCC 2 cut(s) 698, 742
KflI GGGWCCC 1 cut(s) 657
KpnI GGTACC 2 cut(s) 190, 1626
Ksp22I TGATCA 2 cut(s) 450, 1762
KspAI GTTAAC 1 cut(s) 1813
KspI CCGCGG 1 cut(s) 705
Kzo9I GATC 9 cut(s) 149, 428, 450, 484, 1177, 1762, 1831, 1914, 2277
Lsp1109I GCAGC 9 cut(s) 70, 296, 664, 799, 811, 1270, 1670, 1684, 1790
LweI GCATC 3 cut(s) 811, 1360, 1560
MaeI CTAG 4 cut(s) 1556, 1841, 2019, 2262
MaeII ACGT 3 cut(s) 665, 846, 1197
MaeIII GTNAC 3 cut(s) 500, 800, 1689
MalI GATC 9 cut(s) 151, 430, 452, 486, 1179, 1764, 1833, 1916, 2279
MboI GATC 9 cut(s) 149, 428, 450, 484, 1177, 1762, 1831, 1914, 2277
MboII GAAGA 6 cut(s) 38, 60, 144, 182, 1254, 2059
MflI RGATCY 1 cut(s) 149
MhlI GDGCHC 5 cut(s) 728, 779, 1502, 1676, 2123
Mly113I GGCGCC 2 cut(s) 699, 743
MmeI TCCRAC 2 cut(s) 994, 2061
Mph1103I ATGCAT 1 cut(s) 259
MseI TTAA 8 cut(s) 1110, 1191, 1200, 1397, 1608, 1812, 2133, 2148
MslI CAYNNNNRTG 1 cut(s) 1647
MspA1I CMGCKG 6 cut(s) 232, 677, 704, 731, 953, 1697
MspI CCGG 6 cut(s) 137, 276, 681, 1512, 1526, 1542
MspR9I CCNGG 8 cut(s) 277, 330, 682, 1182, 1512, 1513, 1542, 1836
MvaI CCWGG 3 cut(s) 330, 1182, 1836
MvnI CGCG 2 cut(s) 704, 877
NarI GGCGCC 2 cut(s) 699, 743
NciI CCSGG 5 cut(s) 277, 682, 1512, 1513, 1542
NcoI CCATGG 1 cut(s) 998
NdeII GATC 9 cut(s) 149, 428, 450, 484, 1177, 1762, 1831, 1914, 2277
NheI GCTAGC 1 cut(s) 2261
NmuCI GTSAC 3 cut(s) 500, 800, 1689
NsiI ATGCAT 1 cut(s) 259
NspI RCATGY 4 cut(s) 109, 1361, 1463, 1638
OliI CACNNNNGTG 1 cut(s) 1647
PaeI GCATGC 2 cut(s) 109, 1638
PagI TCATGA 3 cut(s) 166, 1296, 1716
PaqCI CACCTGC 1 cut(s) 1226
PciI ACATGT 1 cut(s) 1357
PfeI GAWTC 4 cut(s) 246, 1003, 1085, 1291
PflFI GACNNNGTC 1 cut(s) 2020
PflMI CCANNNNNTGG 2 cut(s) 268, 412
PfoI TCCNGGA 1 cut(s) 1180
PluTI GGCGCC 2 cut(s) 702, 746
PpuMI RGGWCCY 1 cut(s) 657
PscI ACATGT 1 cut(s) 1357
PsiI TTATAA 1 cut(s) 1997
Psp1406I AACGTT 1 cut(s) 1197
Psp5II RGGWCCY 1 cut(s) 657
Psp6I CCWGG 3 cut(s) 328, 1180, 1834
PspEI GGTNACC 1 cut(s) 1689
PspFI CCCAGC 1 cut(s) 2195
PspGI CCWGG 3 cut(s) 328, 1180, 1834
PspPI GGNCC 5 cut(s) 657, 965, 994, 1493, 1538
PspPPI RGGWCCY 1 cut(s) 657
PstI CTGCAG 2 cut(s) 88, 625
PsuI RGATCY 1 cut(s) 149
PsyI GACNNNGTC 1 cut(s) 2020
PvuII CAGCTG 3 cut(s) 232, 677, 953
RsaI GTAC 6 cut(s) 188, 211, 849, 1076, 1624, 1734
RsaNI GTAC 6 cut(s) 187, 210, 848, 1075, 1623, 1733
RseI CAYNNNNRTG 1 cut(s) 1647
SacII CCGCGG 1 cut(s) 705
SaqAI TTAA 8 cut(s) 1110, 1191, 1200, 1397, 1608, 1812, 2133, 2148
Sau3AI GATC 9 cut(s) 149, 428, 450, 484, 1177, 1762, 1831, 1914, 2277
Sau96I GGNCC 5 cut(s) 657, 965, 994, 1493, 1538
SbfI CCTGCAGG 1 cut(s) 625
ScaI AGTACT 1 cut(s) 211
ScrFI CCNGG 8 cut(s) 277, 330, 682, 1182, 1512, 1513, 1542, 1836
SdaI CCTGCAGG 1 cut(s) 625
SduI GDGCHC 5 cut(s) 728, 779, 1502, 1676, 2123
SfaNI GCATC 3 cut(s) 811, 1360, 1560
SfcI CTRYAG 4 cut(s) 84, 621, 766, 1416
SfoI GGCGCC 2 cut(s) 700, 744
Sfr303I CCGCGG 1 cut(s) 705
SgrBI CCGCGG 1 cut(s) 705
SinI GGWCC 4 cut(s) 657, 965, 1493, 1538
SmaI CCCGGG 1 cut(s) 1513
SmiMI CAYNNNNRTG 1 cut(s) 1647
SmlI CTYRAG 2 cut(s) 1667, 1772
SmoI CTYRAG 2 cut(s) 1667, 1772
SphI GCATGC 2 cut(s) 109, 1638
Sse8387I CCTGCAGG 1 cut(s) 625
SsiI CCGC 8 cut(s) 702, 704, 731, 785, 827, 867, 1695, 1725
SspDI GGCGCC 2 cut(s) 698, 742
SspMI CTAG 4 cut(s) 1556, 1841, 2019, 2262
StyD4I CCNGG 8 cut(s) 275, 328, 680, 1180, 1510, 1511, 1540, 1834
StyI CCWWGG 3 cut(s) 406, 862, 998
TaaI ACNGT 4 cut(s) 1012, 1444, 1933, 2044
TaiI ACGT 3 cut(s) 668, 849, 1200
TaqI TCGA 1 cut(s) 236
TaqII GACCGA 1 cut(s) 1510
TatI WGTACW 3 cut(s) 209, 1074, 1732
TauI GCSGC 1 cut(s) 704
TfiI GAWTC 4 cut(s) 246, 1003, 1085, 1291
Tru1I TTAA 8 cut(s) 1110, 1191, 1200, 1397, 1608, 1812, 2133, 2148
Tru9I TTAA 8 cut(s) 1110, 1191, 1200, 1397, 1608, 1812, 2133, 2148
TscAI CASTG 7 cut(s) 84, 1447, 1507, 1540, 1581, 1938, 2049
TseFI GTSAC 3 cut(s) 500, 800, 1689
TseI GCWGC 9 cut(s) 83, 284, 677, 812, 824, 1283, 1658, 1697, 1778
Tsp45I GTSAC 3 cut(s) 500, 800, 1689
TspDTI ATGAA 7 cut(s) 52, 183, 242, 972, 1313, 1473, 1974
TspMI CCCGGG 1 cut(s) 1511
TspRI CASTG 7 cut(s) 84, 1447, 1507, 1540, 1581, 1938, 2049
Tth111I GACNNNGTC 1 cut(s) 2020
Van91I CCANNNNNTGG 2 cut(s) 268, 412
VpaK11BI GGWCC 4 cut(s) 657, 965, 1493, 1538
XapI RAATTY 3 cut(s) 933, 2065, 2135
XceI RCATGY 4 cut(s) 109, 1361, 1463, 1638
XcmI CCANNNNNNNNNTGG 3 cut(s) 528, 1310, 1880
XmaI CCCGGG 1 cut(s) 1511
XmiI GTMKAC 1 cut(s) 1845
XspI CTAG 4 cut(s) 1556, 1841, 2019, 2262
ZrmI AGTACT 1 cut(s) 211
Zsp2I ATGCAT 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.