Prupe.5G085500_v2.0.a1

Peptidase inhibitor I9

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000302
Physical Location & Seq
Forward (+)
9744226 .. 9745313
1088 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G085500.1

Sequence Viewer

Length: 942 bp
ATGTTTTATTTATCACTCGCAGTTGCGCAAGAGGAGAAAAACAATCTGCAAACATATATCGTTTGGGTTCAAAGGCCAGTCTCCCAGAATTCTTTTGCACAATCTCATGAAGATCTGGAGAGTTGGTATCAGTTATTTTTGCCTGAAACCAGTGCAAACTCAAACCAGTTGATGACGCAGAGATTGGTTCATACATACTACAATGTGGCCATAGGGTTTGCAGCAAAACTTACACCAGAGGAAGTGAAGGAAATGGGAAAGAAAGAAGGGTTTGTGTCAGCTCATCCAGAGAGAATTCTGCCTTTGCTCACAATTCATAGTCCTGACTTCTTGGGGTTAGGACTTTGGGAACAAACAAATCATGGTGAAGGTGTGATCATCGGAGTTTTGGATAATGGCGTTGCACCATATCATCCTTCATTTAGTGATGAAGGAGTATCTCCTCATCTGGCTAAACGGAAAGGCAAATGTGACTTCAATGAGACATTCTCAGCTGCTGGAAATTTTGTGGAAGGCGCAAGCGCGTTTGGAGTGGCGAATGGCACAGCTGTTGGCATGGCGCCTTATGCTCACTTGGCCATTTACAGAGTCTGTGGCCTTGATTGTGCTGAAGGTGACGTTTTGGATGCAATGGATGCTGCTGTTCATGATGGTGTGGACATGCTTTCCCTCTCACTTGGCTGGCCCTCAATTCCTTTCTATGAGGATGTGATTGCAGGAATTTTTGTCATCTGTTCAGCTGGAAATTCTGGTCATGAGCATGGTACTTTATCAAATGAAGCTCCATGGATTCTCACCGTTGGAGCAAGCACCACGGACAGAATATTAAACTTAACCTCAACACCTACAGCCACAAACTTGTTCAATGGCTCCACATCAGATCCACTCGCTCCAAAGGTTGCTACATTTTCATCAAGAGGACCAAAGCCCTGGAATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

314

Amino Acids

33.78

Weight (kDa)

4.97

Isoelectric Point (pI)

27.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 27
AccB1I GGYRCC 1 cut(s) 559
AccII CGCG 1 cut(s) 524
AclWI GGATC 1 cut(s) 875
AcoI YGGCCR 2 cut(s) 207, 576
AcsI RAATTY 6 cut(s) 88, 294, 502, 720, 745, 934
AcuI CTGAAG 1 cut(s) 630
AcyI GRCGYC 1 cut(s) 560
AfaI GTAC 1 cut(s) 766
AgsI TTSAA 3 cut(s) 71, 478, 865
AjnI CCWGG 1 cut(s) 929
AluBI AGCT 5 cut(s) 281, 494, 548, 740, 782
AluI AGCT 5 cut(s) 281, 494, 548, 740, 782
Alw26I GTCTC 2 cut(s) 85, 476
AlwI GGATC 1 cut(s) 875
AlwNI CAGNNNCTG 2 cut(s) 497, 591
AoxI GGCC 5 cut(s) 74, 207, 576, 595, 683
ApeKI GCWGC 3 cut(s) 221, 494, 638
ApoI RAATTY 6 cut(s) 88, 294, 502, 720, 745, 934
AspLEI GCGC 4 cut(s) 28, 518, 524, 562
AspS9I GGNCC 2 cut(s) 684, 920
AsuHPI GGTGA 3 cut(s) 377, 626, 787
AvaII GGWCC 1 cut(s) 920
BalI TGGCCA 2 cut(s) 209, 578
BanI GGYRCC 1 cut(s) 559
BbvI GCAGC 3 cut(s) 233, 481, 625
BccI CCATC 1 cut(s) 644
BciT130I CCWGG 1 cut(s) 931
BclI TGATCA 1 cut(s) 375
BcoDI GTCTC 2 cut(s) 85, 476
BfmI CTRYAG 1 cut(s) 846
BfoI RGCGCY 1 cut(s) 563
BglII AGATCT 1 cut(s) 112
BisI GCNGC 3 cut(s) 222, 495, 639
BlsI GCNGC 3 cut(s) 223, 496, 640
Bme1390I CCNGG 1 cut(s) 931
Bme18I GGWCC 1 cut(s) 920
BmgT120I GGNCC 2 cut(s) 684, 920
BmiI GGNNCC 2 cut(s) 561, 871
BmrFI CCNGG 1 cut(s) 931
BmsI GCATC 2 cut(s) 616, 625
BplI GAGNNNNNCTC 2 cut(s) 473, 505
BpmI CTGGAG 1 cut(s) 137
BsaHI GRCGYC 1 cut(s) 560
BsaJI CCNNGG 3 cut(s) 785, 813, 929
BsaXI ACNNNNNCTCC 2 cut(s) 110, 140
Bse1I ACTGG 3 cut(s) 77, 150, 166
Bse3DI GCAATG 1 cut(s) 636
BseBI CCWGG 1 cut(s) 931
BseDI CCNNGG 3 cut(s) 785, 813, 929
BseGI GGATG 5 cut(s) 283, 412, 631, 640, 712
BseMI GCAATG 1 cut(s) 636
BseMII CTCAG 1 cut(s) 504
BseNI ACTGG 3 cut(s) 77, 150, 166
BseRI GAGGAG 2 cut(s) 47, 432
BseXI GCAGC 3 cut(s) 233, 481, 625
Bsh1236I CGCG 1 cut(s) 524
BshFI GGCC 5 cut(s) 76, 209, 578, 597, 685
BshNI GGYRCC 1 cut(s) 559
BsmAI GTCTC 2 cut(s) 85, 476
BsnI GGCC 5 cut(s) 76, 209, 578, 597, 685
Bsp143I GATC 3 cut(s) 112, 375, 880
Bsp19I CCATGG 1 cut(s) 785
BspANI GGCC 5 cut(s) 76, 209, 578, 597, 685
BspCNI CTCAG 1 cut(s) 503
BspFNI CGCG 1 cut(s) 524
BspHI TCATGA 3 cut(s) 106, 646, 754
BspLI GGNNCC 2 cut(s) 561, 871
BspPI GGATC 1 cut(s) 875
BspT107I GGYRCC 1 cut(s) 559
BsrDI GCAATG 1 cut(s) 636
BsrI ACTGG 3 cut(s) 77, 150, 166
BssECI CCNNGG 3 cut(s) 785, 813, 929
BssMI GATC 3 cut(s) 112, 375, 880
BssNI GRCGYC 1 cut(s) 560
BssT1I CCWWGG 1 cut(s) 785
Bst2UI CCWGG 1 cut(s) 931
Bst4CI ACNGT 1 cut(s) 799
BstACI GRCGYC 1 cut(s) 560
BstAPI GCANNNNNTGC 1 cut(s) 635
BstC8I GCNNGC 3 cut(s) 520, 683, 808
BstDEI CTNAG 1 cut(s) 490
BstDSI CCRYGG 2 cut(s) 785, 813
BstF5I GGATG 5 cut(s) 283, 412, 631, 640, 712
BstFNI CGCG 1 cut(s) 524
BstH2I RGCGCY 1 cut(s) 563
BstHHI GCGC 4 cut(s) 28, 518, 524, 562
BstKTI GATC 3 cut(s) 115, 378, 883
BstMAI GTCTC 2 cut(s) 85, 476
BstMBI GATC 3 cut(s) 112, 375, 880
BstMWI GCNNNNNNNGC 3 cut(s) 566, 575, 635
BstNI CCWGG 1 cut(s) 931
BstNSI RCATGY 1 cut(s) 664
BstSCI CCNGG 1 cut(s) 929
BstSFI CTRYAG 1 cut(s) 846
BstUI CGCG 1 cut(s) 524
BstV1I GCAGC 3 cut(s) 233, 481, 625
BstX2I RGATCY 2 cut(s) 112, 880
BstXI CCANNNNNNTGG 1 cut(s) 930
BstYI RGATCY 2 cut(s) 112, 880
BsuRI GGCC 5 cut(s) 76, 209, 578, 597, 685
BtgI CCRYGG 2 cut(s) 785, 813
BtsCI GGATG 5 cut(s) 283, 412, 631, 640, 712
BtsIMutI CAGTG 1 cut(s) 157
Cac8I GCNNGC 3 cut(s) 520, 683, 808
CaiI CAGNNNCTG 2 cut(s) 497, 591
CciI TCATGA 3 cut(s) 106, 646, 754
CfoI GCGC 4 cut(s) 28, 518, 524, 562
Cfr13I GGNCC 2 cut(s) 684, 920
CseI GACGC 1 cut(s) 184
Csp6I GTAC 1 cut(s) 765
CviAII CATG 8 cut(s) 107, 362, 556, 647, 661, 755, 761, 786
CviQI GTAC 1 cut(s) 765
DdeI CTNAG 1 cut(s) 490
DinI GGCGCC 1 cut(s) 561
DpnI GATC 3 cut(s) 114, 377, 882
DpnII GATC 3 cut(s) 112, 375, 880
EaeI YGGCCR 2 cut(s) 207, 576
Eco130I CCWWGG 1 cut(s) 785
Eco47I GGWCC 1 cut(s) 920
Eco57I CTGAAG 1 cut(s) 630
EcoRI GAATTC 3 cut(s) 88, 294, 934
EcoRII CCWGG 1 cut(s) 929
EcoT14I CCWWGG 1 cut(s) 785
EgeI GGCGCC 1 cut(s) 561
EheI GGCGCC 1 cut(s) 561
ErhI CCWWGG 1 cut(s) 785
FaeI CATG 8 cut(s) 110, 365, 559, 650, 664, 758, 764, 789
FatI CATG 8 cut(s) 106, 361, 555, 646, 660, 754, 760, 785
FbaI TGATCA 1 cut(s) 375
Fnu4HI GCNGC 3 cut(s) 222, 495, 639
FokI GGATG 5 cut(s) 270, 399, 638, 647, 719
Fsp4HI GCNGC 3 cut(s) 222, 495, 639
FspI TGCGCA 1 cut(s) 27
GlaI GCGC 4 cut(s) 27, 517, 523, 561
GluI GCNGC 3 cut(s) 222, 495, 639
GsuI CTGGAG 1 cut(s) 137
HaeII RGCGCY 1 cut(s) 563
HaeIII GGCC 5 cut(s) 76, 209, 578, 597, 685
HgaI GACGC 1 cut(s) 184
HhaI GCGC 4 cut(s) 28, 518, 524, 562
Hin1I GRCGYC 1 cut(s) 560
Hin1II CATG 8 cut(s) 110, 365, 559, 650, 664, 758, 764, 789
Hin6I GCGC 4 cut(s) 26, 516, 522, 560
HinP1I GCGC 4 cut(s) 26, 516, 522, 560
HinfI GANTC 2 cut(s) 588, 790
HphI GGTGA 3 cut(s) 377, 626, 787
Hpy166II GTNNAC 1 cut(s) 658
Hpy188I TCNGA 2 cut(s) 383, 880
Hpy188III TCNNGA 7 cut(s) 107, 116, 287, 323, 647, 755, 915
Hpy8I GTNNAC 1 cut(s) 658
HpyAV CCTTC 7 cut(s) 241, 260, 362, 425, 426, 506, 605
HpyCH4III ACNGT 1 cut(s) 799
HpyCH4IV ACGT 1 cut(s) 618
HpyCH4V TGCA 7 cut(s) 49, 98, 155, 221, 404, 629, 716
HpyF10VI GCNNNNNNNGC 3 cut(s) 566, 575, 635
HpyF3I CTNAG 1 cut(s) 490
HpySE526I ACGT 1 cut(s) 618
Hsp92I GRCGYC 1 cut(s) 560
Hsp92II CATG 8 cut(s) 110, 365, 559, 650, 664, 758, 764, 789
HspAI GCGC 4 cut(s) 26, 516, 522, 560
KasI GGCGCC 1 cut(s) 559
Ksp22I TGATCA 1 cut(s) 375
Kzo9I GATC 3 cut(s) 112, 375, 880
LmnI GCTCC 4 cut(s) 787, 803, 875, 895
Lsp1109I GCAGC 3 cut(s) 233, 481, 625
LweI GCATC 2 cut(s) 616, 625
MaeII ACGT 1 cut(s) 618
MaeIII GTNAC 2 cut(s) 470, 614
MalI GATC 3 cut(s) 114, 377, 882
MboI GATC 3 cut(s) 112, 375, 880
MboII GAAGA 1 cut(s) 122
MflI RGATCY 2 cut(s) 112, 880
MlsI TGGCCA 2 cut(s) 209, 578
MluCI AATT 8 cut(s) 88, 294, 312, 502, 690, 720, 745, 934
MluNI TGGCCA 2 cut(s) 209, 578
Mly113I GGCGCC 1 cut(s) 560
MlyI GAGTC 1 cut(s) 597
MmeI TCCRAC 1 cut(s) 781
MnlI CCTC 8 cut(s) 25, 232, 453, 680, 697, 697, 847, 911
Mox20I TGGCCA 2 cut(s) 209, 578
MscI TGGCCA 2 cut(s) 209, 578
MseI TTAA 3 cut(s) 827, 833, 940
MslI CAYNNNNRTG 2 cut(s) 651, 759
Msp20I TGGCCA 2 cut(s) 209, 578
MspA1I CMGCKG 3 cut(s) 494, 548, 740
MspR9I CCNGG 1 cut(s) 931
MvaI CCWGG 1 cut(s) 931
MvnI CGCG 1 cut(s) 524
MwoI GCNNNNNNNGC 3 cut(s) 566, 575, 635
NarI GGCGCC 1 cut(s) 560
NcoI CCATGG 1 cut(s) 785
NdeII GATC 3 cut(s) 112, 375, 880
NlaIII CATG 8 cut(s) 110, 365, 559, 650, 664, 758, 764, 789
NlaIV GGNNCC 2 cut(s) 561, 871
NmuCI GTSAC 2 cut(s) 470, 614
NsbI TGCGCA 1 cut(s) 27
NspI RCATGY 1 cut(s) 664
PagI TCATGA 3 cut(s) 106, 646, 754
PfeI GAWTC 1 cut(s) 790
PkrI GCNGC 3 cut(s) 223, 496, 640
PleI GAGTC 1 cut(s) 596
PluTI GGCGCC 1 cut(s) 563
PpsI GAGTC 1 cut(s) 596
Psp6I CCWGG 1 cut(s) 929
PspGI CCWGG 1 cut(s) 929
PspN4I GGNNCC 2 cut(s) 561, 871
PspPI GGNCC 2 cut(s) 684, 920
PstNI CAGNNNCTG 2 cut(s) 497, 591
PsuI RGATCY 2 cut(s) 112, 880
PvuII CAGCTG 3 cut(s) 494, 548, 740
RsaI GTAC 1 cut(s) 766
RsaNI GTAC 1 cut(s) 765
RseI CAYNNNNRTG 2 cut(s) 651, 759
SaqAI TTAA 3 cut(s) 827, 833, 940
SatI GCNGC 3 cut(s) 222, 495, 639
Sau3AI GATC 3 cut(s) 112, 375, 880
Sau96I GGNCC 2 cut(s) 684, 920
SchI GAGTC 1 cut(s) 597
ScrFI CCNGG 1 cut(s) 931
SfaNI GCATC 2 cut(s) 616, 625
SfcI CTRYAG 1 cut(s) 846
SfoI GGCGCC 1 cut(s) 561
SinI GGWCC 1 cut(s) 920
SmiMI CAYNNNNRTG 2 cut(s) 651, 759
Sse9I AATT 8 cut(s) 88, 294, 312, 502, 690, 720, 745, 934
SspDI GGCGCC 1 cut(s) 559
SspI AATATT 1 cut(s) 825
StyD4I CCNGG 1 cut(s) 929
StyI CCWWGG 1 cut(s) 785
TaaI ACNGT 1 cut(s) 799
TaiI ACGT 1 cut(s) 621
TasI AATT 8 cut(s) 88, 294, 312, 502, 690, 720, 745, 934
TfiI GAWTC 1 cut(s) 790
Tru1I TTAA 3 cut(s) 827, 833, 940
Tru9I TTAA 3 cut(s) 827, 833, 940
TscAI CASTG 1 cut(s) 157
TseFI GTSAC 2 cut(s) 470, 614
TseI GCWGC 3 cut(s) 221, 494, 638
Tsp45I GTSAC 2 cut(s) 470, 614
TspDTI ATGAA 8 cut(s) 123, 179, 305, 408, 444, 635, 792, 900
TspGWI ACGGA 2 cut(s) 472, 830
TspRI CASTG 1 cut(s) 157
VpaK11BI GGWCC 1 cut(s) 920
XapI RAATTY 6 cut(s) 88, 294, 502, 720, 745, 934
XceI RCATGY 1 cut(s) 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.