Rorug07G0058000

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000302
Physical Location & Seq
Forward (+)
4168854 .. 4172875
4022 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0058000.1

Sequence Viewer

Length: 813 bp
ATGGAGTTTTGGAACAGAGCCCGCGGCGTCGCCGAGGAGGCGGCGAAGCGATCGCAGGATCTCACGATCGGCGGCGCGTGGAAGCTCTCCTACATCGTCTCCGACACCGCCAAGCGCTCCAAGGAGATCATCGCCGTCGAGGCCTCCAAGCGAGCCGATCACATCAAGCAATTTGCCGACGCCTTTGCTCCGTCGCTACCGCAGCCACCTGCGGCGGAGAGTCCACAACAACAAGAACAAGAGAAGGATCTGGAAGCGTTTGGAATCACCGAGGAGTTGAGAGACTTCGCTAACGGAATGACTTTTGTCACGTTCAAAGATTTTCCTCTTCCAGATGATACACGATTCTCTGACGTTCCAACAGTCACAAATGTTAGGCAGGATCTTACGGAGTGGCAAGAAAAGCATGCCAATCTTGTTCTTTCAACCGTCGAGGAAATTTCAAAATTAAGATATGAATTGTGTCCACGTGTTATGACGGAAAGGAAATTTTGGAGGATATATTTCATACTAGTTAACAATCATGTCGCACCCTATGAGAAGCGTTATCTGGAGGAAGTTAAGCAAAAGTCAACTGAACAATTTTTAGATGACAGAGTAAAGGAACCTACTAAAGTTGAAACAACTTCTAAAGCAGAGGATAAGGAAGAAAAACATCACACTAAAACTTCAACCTCATCAACTACGGATCAAGACTTGGATGCATTTCTCCTGGGAGACAGTGATGATGATCCAGATCATGATGGTGGGGACTTTGATGATGATTTCGACAAATTAGTGGACGGATCGGATGAAGAGAAGGACAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

270

Amino Acids

31.0

Weight (kDa)

4.68

Isoelectric Point (pI)

47.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 130 - 176 7.1e-11 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 217
Acc36I ACCTGC 1 cut(s) 217
AccII CGCG 2 cut(s) 24, 77
AciI CCGC 8 cut(s) 22, 24, 41, 72, 108, 200, 212, 215
AclWI GGATC 6 cut(s) 66, 255, 390, 696, 725, 793
AcsI RAATTY 2 cut(s) 438, 488
AcvI CACGTG 1 cut(s) 470
AcyI GRCGYC 2 cut(s) 27, 180
AfeI AGCGCT 1 cut(s) 116
AflIII ACRYGT 1 cut(s) 469
AgsI TTSAA 5 cut(s) 316, 426, 444, 620, 672
AhlI ACTAGT 1 cut(s) 511
AjnI CCWGG 1 cut(s) 711
AluBI AGCT 1 cut(s) 85
AluI AGCT 1 cut(s) 85
Alw26I GTCTC 3 cut(s) 103, 276, 711
AlwI GGATC 6 cut(s) 66, 255, 390, 696, 725, 793
Aor51HI AGCGCT 1 cut(s) 116
AoxI GGCC 1 cut(s) 141
ApeKI GCWGC 1 cut(s) 202
ApoI RAATTY 2 cut(s) 438, 488
AsiSI GCGATCGC 1 cut(s) 53
AspLEI GCGC 2 cut(s) 77, 117
AsuHPI GGTGA 1 cut(s) 259
BanII GRGCYC 1 cut(s) 22
BarI GAAGNNNNNNTAC 2 cut(s) 74, 106
BbrPI CACGTG 1 cut(s) 470
BbvI GCAGC 1 cut(s) 214
BccI CCATC 1 cut(s) 737
BceAI ACGGC 1 cut(s) 119
BcgI CGANNNNNNTGC 2 cut(s) 167, 201
BciT130I CCWGG 1 cut(s) 713
BcoDI GTCTC 3 cut(s) 103, 276, 711
BcuI ACTAGT 1 cut(s) 511
BfaI CTAG 1 cut(s) 512
BfoI RGCGCY 1 cut(s) 118
BfuAI ACCTGC 1 cut(s) 217
BglI GCCNNNNNGGC 2 cut(s) 38, 140
BisI GCNGC 5 cut(s) 25, 42, 73, 203, 213
BlsI GCNGC 5 cut(s) 26, 43, 74, 204, 214
Bme1390I CCNGG 1 cut(s) 713
BmiI GGNNCC 1 cut(s) 606
BmrFI CCNGG 1 cut(s) 713
BmsI GCATC 1 cut(s) 691
BoxI GACNNNNGTC 1 cut(s) 305
BpmI CTGGAG 1 cut(s) 572
BsaAI YACGTR 1 cut(s) 470
BsaBI GATNNNNATC 2 cut(s) 729, 735
BsaHI GRCGYC 2 cut(s) 27, 180
BsaJI CCNNGG 5 cut(s) 22, 33, 120, 270, 712
BsaXI ACNNNNNCTCC 2 cut(s) 83, 113
Bse8I GATNNNNATC 2 cut(s) 729, 735
BseBI CCWGG 1 cut(s) 713
BseDI CCNNGG 5 cut(s) 22, 33, 120, 270, 712
BseGI GGATG 2 cut(s) 706, 796
BseJI GATNNNNATC 2 cut(s) 729, 735
BseRI GAGGAG 2 cut(s) 50, 287
BseXI GCAGC 1 cut(s) 214
Bsh1236I CGCG 2 cut(s) 24, 77
Bsh1285I CGRYCG 2 cut(s) 53, 69
BshFI GGCC 1 cut(s) 143
BsiEI CGRYCG 2 cut(s) 53, 69
BslFI GGGAC 1 cut(s) 764
BsmAI GTCTC 3 cut(s) 103, 276, 711
BsmBI CGTCTC 1 cut(s) 103
BsmFI GGGAC 1 cut(s) 764
BsnI GGCC 1 cut(s) 143
Bsp1286I GDGCHC 1 cut(s) 22
BspACI CCGC 8 cut(s) 22, 24, 41, 72, 108, 200, 212, 215
BspANI GGCC 1 cut(s) 143
BspFNI CGCG 2 cut(s) 24, 77
BspHI TCATGA 1 cut(s) 739
BspLI GGNNCC 1 cut(s) 606
BspMI ACCTGC 1 cut(s) 217
BspPI GGATC 6 cut(s) 66, 255, 390, 696, 725, 793
BssECI CCNNGG 5 cut(s) 22, 33, 120, 270, 712
BssNI GRCGYC 2 cut(s) 27, 180
BssT1I CCWWGG 1 cut(s) 120
Bst2UI CCWGG 1 cut(s) 713
Bst4CI ACNGT 3 cut(s) 364, 430, 722
Bst6I CTCTTC 2 cut(s) 333, 789
BstACI GRCGYC 2 cut(s) 27, 180
BstBAI YACGTR 1 cut(s) 470
BstC8I GCNNGC 3 cut(s) 22, 153, 408
BstDSI CCRYGG 1 cut(s) 22
BstF5I GGATG 2 cut(s) 706, 796
BstFNI CGCG 2 cut(s) 24, 77
BstH2I RGCGCY 1 cut(s) 118
BstHHI GCGC 2 cut(s) 77, 117
BstMAI GTCTC 3 cut(s) 103, 276, 711
BstMCI CGRYCG 2 cut(s) 53, 69
BstMWI GCNNNNNNNGC 4 cut(s) 38, 140, 202, 403
BstNI CCWGG 1 cut(s) 713
BstNSI RCATGY 1 cut(s) 410
BstPAI GACNNNNGTC 1 cut(s) 305
BstSCI CCNGG 1 cut(s) 711
BstUI CGCG 2 cut(s) 24, 77
BstV1I GCAGC 1 cut(s) 214
BstX2I RGATCY 3 cut(s) 58, 247, 382
BstYI RGATCY 3 cut(s) 58, 247, 382
BsuRI GGCC 1 cut(s) 143
BtgI CCRYGG 1 cut(s) 22
BtgZI GCGATG 1 cut(s) 115
BtsCI GGATG 2 cut(s) 706, 796
BtsIMutI CAGTG 1 cut(s) 727
BveI ACCTGC 1 cut(s) 217
Cac8I GCNNGC 3 cut(s) 22, 153, 408
CciI TCATGA 1 cut(s) 739
CfoI GCGC 2 cut(s) 77, 117
Cfr42I CCGCGG 1 cut(s) 25
CseI GACGC 2 cut(s) 16, 188
CviAII CATG 3 cut(s) 407, 524, 740
CviJI RGCY 5 cut(s) 20, 85, 143, 155, 205
CviKI_1 RGCY 5 cut(s) 20, 85, 143, 155, 205
Eam1104I CTCTTC 2 cut(s) 333, 789
EarI CTCTTC 2 cut(s) 333, 789
EciI GGCGGA 1 cut(s) 230
Eco130I CCWWGG 1 cut(s) 120
Eco147I AGGCCT 1 cut(s) 143
Eco24I GRGCYC 1 cut(s) 22
Eco47III AGCGCT 1 cut(s) 116
Eco72I CACGTG 1 cut(s) 470
EcoRII CCWGG 1 cut(s) 711
EcoT14I CCWWGG 1 cut(s) 120
EcoT22I ATGCAT 1 cut(s) 706
EcoT38I GRGCYC 1 cut(s) 22
ErhI CCWWGG 1 cut(s) 120
Esp3I CGTCTC 1 cut(s) 103
FaeI CATG 3 cut(s) 410, 527, 743
FaiI YATR 8 cut(s) 408, 456, 476, 502, 509, 525, 537, 741
FaqI GGGAC 1 cut(s) 764
FatI CATG 3 cut(s) 406, 523, 739
FauI CCCGC 1 cut(s) 29
Fnu4HI GCNGC 5 cut(s) 25, 42, 73, 203, 213
FokI GGATG 2 cut(s) 713, 803
FriOI GRGCYC 1 cut(s) 22
Fsp4HI GCNGC 5 cut(s) 25, 42, 73, 203, 213
FspBI CTAG 1 cut(s) 512
GlaI GCGC 2 cut(s) 76, 116
GluI GCNGC 5 cut(s) 25, 42, 73, 203, 213
GsuI CTGGAG 1 cut(s) 572
HaeII RGCGCY 1 cut(s) 118
HaeIII GGCC 1 cut(s) 143
HgaI GACGC 2 cut(s) 16, 188
HhaI GCGC 2 cut(s) 77, 117
Hin1I GRCGYC 2 cut(s) 27, 180
Hin1II CATG 3 cut(s) 410, 527, 743
Hin6I GCGC 2 cut(s) 75, 115
HinP1I GCGC 2 cut(s) 75, 115
HincII GTYRAC 2 cut(s) 517, 573
HindII GTYRAC 2 cut(s) 517, 573
HinfI GANTC 3 cut(s) 220, 264, 345
HpaI GTTAAC 1 cut(s) 517
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 5 cut(s) 224, 467, 517, 573, 781
Hpy188I TCNGA 3 cut(s) 103, 352, 790
Hpy188III TCNNGA 7 cut(s) 64, 251, 332, 551, 692, 734, 740
Hpy8I GTNNAC 5 cut(s) 224, 467, 517, 573, 781
Hpy99I CGWCG 5 cut(s) 32, 140, 182, 196, 434
HpyAV CCTTC 2 cut(s) 238, 793
HpyCH4III ACNGT 3 cut(s) 364, 430, 722
HpyCH4IV ACGT 3 cut(s) 311, 354, 469
HpyCH4V TGCA 1 cut(s) 704
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 140, 202, 403
HpySE526I ACGT 3 cut(s) 311, 354, 469
Hsp92I GRCGYC 2 cut(s) 27, 180
Hsp92II CATG 3 cut(s) 410, 527, 743
HspAI GCGC 2 cut(s) 75, 115
KspAI GTTAAC 1 cut(s) 517
KspI CCGCGG 1 cut(s) 25
LmnI GCTCC 2 cut(s) 122, 193
LpnPI CCDG 9 cut(s) 41, 222, 236, 345, 365, 536, 698, 725, 747
Lsp1109I GCAGC 1 cut(s) 214
LweI GCATC 1 cut(s) 691
MaeI CTAG 1 cut(s) 512
MaeII ACGT 3 cut(s) 311, 354, 469
MaeIII GTNAC 2 cut(s) 307, 364
MboII GAAGA 3 cut(s) 320, 659, 806
MflI RGATCY 3 cut(s) 58, 247, 382
MhlI GDGCHC 1 cut(s) 22
MluCI AATT 7 cut(s) 170, 438, 446, 458, 488, 581, 773
MlyI GAGTC 1 cut(s) 229
MmeI TCCRAC 2 cut(s) 126, 383
Mph1103I ATGCAT 1 cut(s) 706
MseI TTAA 3 cut(s) 449, 516, 561
MslI CAYNNNNRTG 1 cut(s) 744
MspA1I CMGCKG 1 cut(s) 24
MspR9I CCNGG 1 cut(s) 713
MvaI CCWGG 1 cut(s) 713
MvnI CGCG 2 cut(s) 24, 77
MwoI GCNNNNNNNGC 4 cut(s) 38, 140, 202, 403
NlaIII CATG 3 cut(s) 410, 527, 743
NlaIV GGNNCC 1 cut(s) 606
NmeAIII GCCGAG 1 cut(s) 58
NmuCI GTSAC 2 cut(s) 307, 364
NsiI ATGCAT 1 cut(s) 706
NspI RCATGY 1 cut(s) 410
PaeI GCATGC 1 cut(s) 410
PagI TCATGA 1 cut(s) 739
PaqCI CACCTGC 1 cut(s) 217
PceI AGGCCT 1 cut(s) 143
PfeI GAWTC 2 cut(s) 264, 345
PkrI GCNGC 5 cut(s) 26, 43, 74, 204, 214
Ple19I CGATCG 2 cut(s) 53, 69
PleI GAGTC 1 cut(s) 228
PmaCI CACGTG 1 cut(s) 470
PmlI CACGTG 1 cut(s) 470
PpsI GAGTC 1 cut(s) 228
Ppu21I YACGTR 1 cut(s) 470
PshAI GACNNNNGTC 1 cut(s) 305
Psp6I CCWGG 1 cut(s) 711
PspCI CACGTG 1 cut(s) 470
PspGI CCWGG 1 cut(s) 711
PspN4I GGNNCC 1 cut(s) 606
PsuI RGATCY 3 cut(s) 58, 247, 382
PvuI CGATCG 2 cut(s) 53, 69
RgaI GCGATCGC 1 cut(s) 53
RseI CAYNNNNRTG 1 cut(s) 744
SacII CCGCGG 1 cut(s) 25
SaqAI TTAA 3 cut(s) 449, 516, 561
SatI GCNGC 5 cut(s) 25, 42, 73, 203, 213
SchI GAGTC 1 cut(s) 229
ScrFI CCNGG 1 cut(s) 713
SduI GDGCHC 1 cut(s) 22
SetI ASST 7 cut(s) 87, 211, 314, 357, 472, 610, 677
SfaAI GCGATCGC 1 cut(s) 53
SfaNI GCATC 1 cut(s) 691
Sfr303I CCGCGG 1 cut(s) 25
SgfI GCGATCGC 1 cut(s) 53
SgrBI CCGCGG 1 cut(s) 25
SmiMI CAYNNNNRTG 1 cut(s) 744
SpeI ACTAGT 1 cut(s) 511
SphI GCATGC 1 cut(s) 410
Sse9I AATT 7 cut(s) 170, 438, 446, 458, 488, 581, 773
SseBI AGGCCT 1 cut(s) 143
SsiI CCGC 8 cut(s) 22, 24, 41, 72, 108, 200, 212, 215
SspMI CTAG 1 cut(s) 512
StuI AGGCCT 1 cut(s) 143
StyD4I CCNGG 1 cut(s) 711
StyI CCWWGG 1 cut(s) 120
TaaI ACNGT 3 cut(s) 364, 430, 722
TaiI ACGT 3 cut(s) 314, 357, 472
TaqI TCGA 3 cut(s) 138, 432, 768
TasI AATT 7 cut(s) 170, 438, 446, 458, 488, 581, 773
TauI GCSGC 4 cut(s) 27, 44, 75, 215
TfiI GAWTC 2 cut(s) 264, 345
Tru1I TTAA 3 cut(s) 449, 516, 561
Tru9I TTAA 3 cut(s) 449, 516, 561
TscAI CASTG 1 cut(s) 727
TseFI GTSAC 2 cut(s) 307, 364
TseI GCWGC 1 cut(s) 202
Tsp45I GTSAC 2 cut(s) 307, 364
TspDTI ATGAA 3 cut(s) 471, 496, 807
TspGWI ACGGA 6 cut(s) 180, 309, 404, 494, 701, 798
TspRI CASTG 1 cut(s) 727
XapI RAATTY 2 cut(s) 438, 488
XceI RCATGY 1 cut(s) 410
XspI CTAG 1 cut(s) 512
Zsp2I ATGCAT 1 cut(s) 706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.