MD04G1071800.v1.1

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000302
Physical Location & Seq
Reverse (-)
9706269 .. 9706691
423 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1071800.v1.1.491

Sequence Viewer

Length: 423 bp
ATGGAGAACAAAAAAGGTGCACCAACGCATGTCATATATCTTCTTGGTTTTACTTTGATCATGTTTTACTTATCAAAGGCAGTTGCACAAGAGGAAAAAAATAGCCTTCAAACTTACATTGTTTTTCTCGAAAAGCCGGCCTCCTCGAAATTTTCTGAAGAATCAGATTTAGATAGTTGGTATCAGTCATTTTTGCCTGAAACTACGGCAAACTTAAACCAGCAGAGGATCGTTCATGCCTACTGCAGTGTGGTCACTGGATTTGCAGCTAAACTGACACCAGAGGAAGTGAAAGCAATGGAAAACAAAGAAGGGTTTTTGTCGGCTCATCTCGAGAAAACGGTTCCTTTGCTAACTACTCATAGTCCTGACTTCTTGGGGTTAAACCAAGGATGCGGACTTTCCACAAGCAAATTACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

141

Amino Acids

15.64

Weight (kDa)

6.07

Isoelectric Point (pI)

40.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 38 - 117 1.1e-10 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 396
AclWI GGATC 1 cut(s) 236
AcsI RAATTY 1 cut(s) 149
AcuI CTGAAG 1 cut(s) 177
AgsI TTSAA 1 cut(s) 110
AluBI AGCT 1 cut(s) 269
AluI AGCT 1 cut(s) 269
Alw21I GWGCWC 1 cut(s) 22
Alw44I GTGCAC 1 cut(s) 18
AlwI GGATC 1 cut(s) 236
Ama87I CYCGRG 1 cut(s) 332
AoxI GGCC 1 cut(s) 138
ApaLI GTGCAC 1 cut(s) 18
ApeKI GCWGC 1 cut(s) 266
ApoI RAATTY 1 cut(s) 149
AvaI CYCGRG 1 cut(s) 332
BaeGI GKGCMC 1 cut(s) 22
Bbv12I GWGCWC 1 cut(s) 22
BbvI GCAGC 1 cut(s) 278
BceAI ACGGC 1 cut(s) 222
BclI TGATCA 1 cut(s) 57
BfmI CTRYAG 1 cut(s) 244
BisI GCNGC 1 cut(s) 267
BlsI GCNGC 1 cut(s) 268
BmeT110I CYCGRG 1 cut(s) 332
BmiI GGNNCC 1 cut(s) 345
BmsI GCATC 1 cut(s) 383
BsaJI CCNNGG 1 cut(s) 388
Bse118I RCCGGY 1 cut(s) 136
Bse1I ACTGG 1 cut(s) 262
Bse3DI GCAATG 1 cut(s) 303
BseDI CCNNGG 1 cut(s) 388
BseGI GGATG 1 cut(s) 398
BseMI GCAATG 1 cut(s) 303
BseNI ACTGG 1 cut(s) 262
BseRI GAGGAG 1 cut(s) 133
BseSI GKGCMC 1 cut(s) 22
BseXI GCAGC 1 cut(s) 278
BshFI GGCC 1 cut(s) 140
BsiHKAI GWGCWC 1 cut(s) 22
BsiHKCI CYCGRG 1 cut(s) 332
BsiSI CCGG 1 cut(s) 137
BsnI GGCC 1 cut(s) 140
BsoBI CYCGRG 1 cut(s) 332
Bsp1286I GDGCHC 1 cut(s) 22
Bsp143I GATC 2 cut(s) 57, 228
BspACI CCGC 1 cut(s) 396
BspANI GGCC 1 cut(s) 140
BspLI GGNNCC 1 cut(s) 345
BspMAI CTGCAG 1 cut(s) 248
BspPI GGATC 1 cut(s) 236
BsrDI GCAATG 1 cut(s) 303
BsrFI RCCGGY 1 cut(s) 136
BsrI ACTGG 1 cut(s) 262
BssAI RCCGGY 1 cut(s) 136
BssECI CCNNGG 1 cut(s) 388
BssMI GATC 2 cut(s) 57, 228
BssT1I CCWWGG 1 cut(s) 388
Bst4CI ACNGT 2 cut(s) 343, 420
BstC8I GCNNGC 1 cut(s) 138
BstF5I GGATG 1 cut(s) 398
BstKTI GATC 2 cut(s) 60, 231
BstMBI GATC 2 cut(s) 57, 228
BstNSI RCATGY 1 cut(s) 32
BstSFI CTRYAG 1 cut(s) 244
BstSLI GKGCMC 1 cut(s) 22
BstV1I GCAGC 1 cut(s) 278
BsuRI GGCC 1 cut(s) 140
BtsCI GGATG 1 cut(s) 398
BtsI GCAGTG 1 cut(s) 253
BtsIMutI CAGTG 2 cut(s) 253, 255
Cac8I GCNNGC 1 cut(s) 138
Cfr10I RCCGGY 1 cut(s) 136
CviAII CATG 3 cut(s) 29, 61, 236
CviJI RGCY 5 cut(s) 105, 136, 140, 269, 326
CviKI_1 RGCY 5 cut(s) 105, 136, 140, 269, 326
DpnI GATC 2 cut(s) 59, 230
DpnII GATC 2 cut(s) 57, 228
Eco130I CCWWGG 1 cut(s) 388
Eco57I CTGAAG 1 cut(s) 177
Eco88I CYCGRG 1 cut(s) 332
EcoT14I CCWWGG 1 cut(s) 388
ErhI CCWWGG 1 cut(s) 388
FaeI CATG 3 cut(s) 32, 64, 239
FaiI YATR 6 cut(s) 30, 35, 37, 62, 237, 363
FatI CATG 3 cut(s) 28, 60, 235
FbaI TGATCA 1 cut(s) 57
Fnu4HI GCNGC 1 cut(s) 267
FokI GGATG 1 cut(s) 405
Fsp4HI GCNGC 1 cut(s) 267
GluI GCNGC 1 cut(s) 267
HaeIII GGCC 1 cut(s) 140
HapII CCGG 1 cut(s) 137
Hin1II CATG 3 cut(s) 32, 64, 239
HinfI GANTC 1 cut(s) 161
HpaII CCGG 1 cut(s) 137
Hpy166II GTNNAC 1 cut(s) 20
Hpy188I TCNGA 2 cut(s) 157, 166
Hpy188III TCNNGA 4 cut(s) 128, 332, 334, 368
Hpy8I GTNNAC 1 cut(s) 20
HpyAV CCTTC 2 cut(s) 116, 305
HpyCH4III ACNGT 2 cut(s) 343, 420
HpyCH4V TGCA 4 cut(s) 20, 86, 246, 266
Hsp92II CATG 3 cut(s) 32, 64, 239
KroI GCCGGC 1 cut(s) 136
KroNI GCCGGC 1 cut(s) 138
Ksp22I TGATCA 1 cut(s) 57
Kzo9I GATC 2 cut(s) 57, 228
LpnPI CCDG 6 cut(s) 150, 210, 233, 243, 294, 381
Lsp1109I GCAGC 1 cut(s) 278
LweI GCATC 1 cut(s) 383
MaeIII GTNAC 1 cut(s) 253
MalI GATC 2 cut(s) 59, 230
MboI GATC 2 cut(s) 57, 228
MboII GAAGA 2 cut(s) 32, 170
MhlI GDGCHC 1 cut(s) 22
MluCI AATT 2 cut(s) 149, 413
MnlI CCTC 5 cut(s) 85, 151, 154, 219, 277
MroNI GCCGGC 1 cut(s) 136
MseI TTAA 2 cut(s) 215, 383
MspI CCGG 1 cut(s) 137
NaeI GCCGGC 1 cut(s) 138
NdeII GATC 2 cut(s) 57, 228
NgoMIV GCCGGC 1 cut(s) 136
NlaIII CATG 3 cut(s) 32, 64, 239
NlaIV GGNNCC 1 cut(s) 345
NmuCI GTSAC 1 cut(s) 253
NspI RCATGY 1 cut(s) 32
PaeR7I CTCGAG 1 cut(s) 332
PdiI GCCGGC 1 cut(s) 138
PfeI GAWTC 1 cut(s) 161
PkrI GCNGC 1 cut(s) 268
PspN4I GGNNCC 1 cut(s) 345
PstI CTGCAG 1 cut(s) 248
SaqAI TTAA 2 cut(s) 215, 383
SatI GCNGC 1 cut(s) 267
Sau3AI GATC 2 cut(s) 57, 228
SduI GDGCHC 1 cut(s) 22
SetI ASST 2 cut(s) 19, 271
SfaNI GCATC 1 cut(s) 383
SfcI CTRYAG 1 cut(s) 244
Sfr274I CTCGAG 1 cut(s) 332
SlaI CTCGAG 1 cut(s) 332
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
Sse9I AATT 2 cut(s) 149, 413
SsiI CCGC 1 cut(s) 396
StyI CCWWGG 1 cut(s) 388
TaaI ACNGT 2 cut(s) 343, 420
TaqI TCGA 3 cut(s) 129, 146, 333
TasI AATT 2 cut(s) 149, 413
TfiI GAWTC 1 cut(s) 161
Tru1I TTAA 2 cut(s) 215, 383
Tru9I TTAA 2 cut(s) 215, 383
TscAI CASTG 2 cut(s) 253, 262
TseFI GTSAC 1 cut(s) 253
TseI GCWGC 1 cut(s) 266
Tsp45I GTSAC 1 cut(s) 253
TspDTI ATGAA 1 cut(s) 224
TspRI CASTG 2 cut(s) 253, 262
VneI GTGCAC 1 cut(s) 18
XapI RAATTY 1 cut(s) 149
XceI RCATGY 1 cut(s) 32
XhoI CTCGAG 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.