subtilisin-like protease
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 609 |
| AarI | CACCTGC | 3 cut(s) | 917, 1016, 1135 |
| Acc36I | ACCTGC | 3 cut(s) | 917, 1016, 1135 |
| Acc65I | GGTACC | 2 cut(s) | 374, 1657 |
| AccB1I | GGYRCC | 6 cut(s) | 311, 374, 416, 460, 1283, 1657 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 34, 178 |
| AccBSI | CCGCTC | 1 cut(s) | 110 |
| AccII | CGCG | 1 cut(s) | 1467 |
| AciI | CCGC | 5 cut(s) | 108, 1465, 1467, 1493, 1853 |
| AclWI | GGATC | 2 cut(s) | 341, 937 |
| AcuI | CTGAAG | 2 cut(s) | 1005, 1554 |
| AcyI | GRCGYC | 1 cut(s) | 417 |
| AdeI | CACNNNGTG | 2 cut(s) | 34, 584 |
| AfaI | GTAC | 4 cut(s) | 376, 485, 1395, 1659 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 34, 178, 296, 692, 1754 |
| AgsI | TTSAA | 6 cut(s) | 710, 816, 854, 1096, 1183, 1846 |
| AjiI | CACGTC | 1 cut(s) | 565 |
| AjnI | CCWGG | 3 cut(s) | 247, 1171, 1731 |
| AleI | CACNNNNGTG | 1 cut(s) | 1308 |
| Alw21I | GWGCWC | 2 cut(s) | 570, 1337 |
| Alw26I | GTCTC | 3 cut(s) | 80, 1593, 1807 |
| AlwI | GGATC | 2 cut(s) | 341, 937 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 452 |
| AoxI | GGCC | 2 cut(s) | 1226, 1621 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1089, 1121 |
| Asp718I | GGTACC | 2 cut(s) | 374, 1657 |
| AspLEI | GCGC | 2 cut(s) | 419, 1540 |
| AspS9I | GGNCC | 5 cut(s) | 683, 1154, 1199, 1436, 1622 |
| AsuC2I | CCSGG | 1 cut(s) | 1203 |
| AsuHPI | GGTGA | 6 cut(s) | 218, 920, 1124, 1295, 1344, 1621 |
| AvaII | GGWCC | 4 cut(s) | 683, 1154, 1199, 1436 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 358, 391 |
| BanI | GGYRCC | 6 cut(s) | 311, 374, 416, 460, 1283, 1657 |
| BanII | GRGCYC | 2 cut(s) | 1172, 1337 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1712, 1744 |
| Bbv12I | GWGCWC | 2 cut(s) | 570, 1337 |
| BccI | CCATC | 4 cut(s) | 89, 791, 839, 1791 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1090, 1124 |
| BciT130I | CCWGG | 3 cut(s) | 249, 1173, 1733 |
| BclI | TGATCA | 3 cut(s) | 499, 1114, 1807 |
| BcnI | CCSGG | 1 cut(s) | 1203 |
| BcoDI | GTCTC | 3 cut(s) | 80, 1593, 1807 |
| BfaI | CTAG | 2 cut(s) | 116, 1824 |
| BfmI | CTRYAG | 4 cut(s) | 970, 1023, 1128, 1257 |
| BfoI | RGCGCY | 1 cut(s) | 420 |
| BfuAI | ACCTGC | 3 cut(s) | 917, 1016, 1135 |
| BlpI | GCTNAGC | 3 cut(s) | 592, 667, 1216 |
| BmcAI | AGTACT | 1 cut(s) | 1395 |
| Bme1390I | CCNGG | 4 cut(s) | 249, 1173, 1203, 1733 |
| Bme18I | GGWCC | 4 cut(s) | 683, 1154, 1199, 1436 |
| BmgBI | CACGTC | 1 cut(s) | 565 |
| BmgT120I | GGNCC | 5 cut(s) | 683, 1154, 1199, 1436, 1622 |
| BmrFI | CCNGG | 4 cut(s) | 249, 1173, 1203, 1733 |
| BmrI | ACTGGG | 1 cut(s) | 1656 |
| BmsI | GCATC | 2 cut(s) | 1044, 1759 |
| BmuI | ACTGGG | 1 cut(s) | 1656 |
| Bpu10I | CCTNAGC | 1 cut(s) | 979 |
| Bpu1102I | GCTNAGC | 3 cut(s) | 592, 667, 1216 |
| BpuEI | CTTGAG | 4 cut(s) | 1313, 1374, 1500, 1575 |
| BpuMI | CCSGG | 1 cut(s) | 1203 |
| BsaHI | GRCGYC | 1 cut(s) | 417 |
| BsaI | GGTCTC | 1 cut(s) | 1807 |
| BsaJI | CCNNGG | 7 cut(s) | 172, 325, 370, 716, 1171, 1402, 1465 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 549, 554, 579, 584, 642, 672 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 34, 178, 296, 692, 1754 |
| Bse1I | ACTGG | 3 cut(s) | 46, 353, 1662 |
| Bse3DI | GCAATG | 3 cut(s) | 486, 835, 1314 |
| BseBI | CCWGG | 3 cut(s) | 249, 1173, 1733 |
| BseDI | CCNNGG | 7 cut(s) | 172, 325, 370, 716, 1171, 1402, 1465 |
| BseGI | GGATG | 1 cut(s) | 1774 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 34, 178, 296, 692, 1754 |
| BseMI | GCAATG | 3 cut(s) | 486, 835, 1314 |
| BseMII | CTCAG | 5 cut(s) | 606, 681, 1298, 1680, 1784 |
| BseNI | ACTGG | 3 cut(s) | 46, 353, 1662 |
| BseRI | GAGGAG | 1 cut(s) | 276 |
| BseYI | CCCAGC | 1 cut(s) | 1624 |
| BsgI | GTGCAG | 1 cut(s) | 1779 |
| Bsh1236I | CGCG | 1 cut(s) | 1467 |
| BshFI | GGCC | 2 cut(s) | 1228, 1623 |
| BshNI | GGYRCC | 6 cut(s) | 311, 374, 416, 460, 1283, 1657 |
| BsiHKAI | GWGCWC | 2 cut(s) | 570, 1337 |
| BsiSI | CCGG | 3 cut(s) | 290, 315, 1203 |
| BslFI | GGGAC | 2 cut(s) | 195, 1185 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 34, 178, 296, 692, 1754 |
| BsmAI | GTCTC | 3 cut(s) | 80, 1593, 1807 |
| BsmFI | GGGAC | 2 cut(s) | 195, 1185 |
| BsmI | GAATGC | 1 cut(s) | 1813 |
| BsnI | GGCC | 2 cut(s) | 1228, 1623 |
| Bso31I | GGTCTC | 1 cut(s) | 1807 |
| Bsp1286I | GDGCHC | 3 cut(s) | 570, 1172, 1337 |
| Bsp143I | GATC | 6 cut(s) | 346, 499, 942, 1114, 1807, 1839 |
| Bsp1720I | GCTNAGC | 3 cut(s) | 592, 667, 1216 |
| Bsp19I | CCATGG | 2 cut(s) | 370, 716 |
| BspACI | CCGC | 5 cut(s) | 108, 1465, 1467, 1493, 1853 |
| BspANI | GGCC | 2 cut(s) | 1228, 1623 |
| BspCNI | CTCAG | 5 cut(s) | 605, 680, 1299, 1681, 1785 |
| BspFNI | CGCG | 1 cut(s) | 1467 |
| BspMAI | CTGCAG | 2 cut(s) | 1027, 1132 |
| BspMI | ACCTGC | 3 cut(s) | 917, 1016, 1135 |
| BspPI | GGATC | 2 cut(s) | 341, 937 |
| BspT107I | GGYRCC | 6 cut(s) | 311, 374, 416, 460, 1283, 1657 |
| BspTNI | GGTCTC | 1 cut(s) | 1807 |
| BsrBI | CCGCTC | 1 cut(s) | 110 |
| BsrDI | GCAATG | 3 cut(s) | 486, 835, 1314 |
| BsrI | ACTGG | 3 cut(s) | 46, 353, 1662 |
| BssECI | CCNNGG | 7 cut(s) | 172, 325, 370, 716, 1171, 1402, 1465 |
| BssMI | GATC | 6 cut(s) | 346, 499, 942, 1114, 1807, 1839 |
| BssNI | GRCGYC | 1 cut(s) | 417 |
| BssT1I | CCWWGG | 4 cut(s) | 172, 370, 716, 1402 |
| Bst2UI | CCWGG | 3 cut(s) | 249, 1173, 1733 |
| Bst4CI | ACNGT | 5 cut(s) | 506, 730, 1055, 1105, 1609 |
| BstACI | GRCGYC | 1 cut(s) | 417 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 626, 932 |
| BstC8I | GCNNGC | 3 cut(s) | 739, 1168, 1469 |
| BstDEI | CTNAG | 8 cut(s) | 592, 667, 979, 1216, 1307, 1483, 1689, 1793 |
| BstDSI | CCRYGG | 3 cut(s) | 370, 716, 1465 |
| BstEII | GGTNACC | 1 cut(s) | 1350 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1752 |
| BstF5I | GGATG | 1 cut(s) | 1774 |
| BstFNI | CGCG | 1 cut(s) | 1467 |
| BstH2I | RGCGCY | 1 cut(s) | 420 |
| BstHHI | GCGC | 2 cut(s) | 419, 1540 |
| BstKTI | GATC | 6 cut(s) | 349, 502, 945, 1117, 1810, 1842 |
| BstMAI | GTCTC | 3 cut(s) | 80, 1593, 1807 |
| BstMBI | GATC | 6 cut(s) | 346, 499, 942, 1114, 1807, 1839 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 467, 476, 626, 635, 932, 1167 |
| BstNI | CCWGG | 3 cut(s) | 249, 1173, 1733 |
| BstNSI | RCATGY | 1 cut(s) | 387 |
| BstPI | GGTNACC | 1 cut(s) | 1350 |
| BstSCI | CCNGG | 4 cut(s) | 247, 1171, 1201, 1731 |
| BstSFI | CTRYAG | 4 cut(s) | 970, 1023, 1128, 1257 |
| BstUI | CGCG | 1 cut(s) | 1467 |
| BstX2I | RGATCY | 1 cut(s) | 346 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 559 |
| BstYI | RGATCY | 1 cut(s) | 346 |
| BsuRI | GGCC | 2 cut(s) | 1228, 1623 |
| BtgI | CCRYGG | 3 cut(s) | 370, 716, 1465 |
| BtgZI | GCGATG | 1 cut(s) | 282 |
| BtrI | CACGTC | 1 cut(s) | 565 |
| BtsCI | GGATG | 1 cut(s) | 1774 |
| BtsI | GCAGTG | 1 cut(s) | 540 |
| BtsIMutI | CAGTG | 3 cut(s) | 540, 1101, 1455 |
| BveI | ACCTGC | 3 cut(s) | 917, 1016, 1135 |
| Cac8I | GCNNGC | 3 cut(s) | 739, 1168, 1469 |
| CaiI | CAGNNNCTG | 1 cut(s) | 452 |
| CfoI | GCGC | 2 cut(s) | 419, 1540 |
| Cfr13I | GGNCC | 5 cut(s) | 683, 1154, 1199, 1436, 1622 |
| Cfr42I | CCGCGG | 1 cut(s) | 1468 |
| CseI | GACGC | 1 cut(s) | 438 |
| CsiI | ACCWGGT | 2 cut(s) | 247, 1731 |
| Csp6I | GTAC | 4 cut(s) | 375, 484, 1394, 1658 |
| CviQI | GTAC | 4 cut(s) | 375, 484, 1394, 1658 |
| DdeI | CTNAG | 8 cut(s) | 592, 667, 979, 1216, 1307, 1483, 1689, 1793 |
| DinI | GGCGCC | 1 cut(s) | 418 |
| DpnI | GATC | 6 cut(s) | 348, 501, 944, 1116, 1809, 1841 |
| DpnII | GATC | 6 cut(s) | 346, 499, 942, 1114, 1807, 1839 |
| DraIII | CACNNNGTG | 2 cut(s) | 34, 584 |
| Ecl136II | GAGCTC | 1 cut(s) | 1335 |
| Eco130I | CCWWGG | 4 cut(s) | 172, 370, 716, 1402 |
| Eco24I | GRGCYC | 2 cut(s) | 1172, 1337 |
| Eco31I | GGTCTC | 1 cut(s) | 1807 |
| Eco32I | GATATC | 1 cut(s) | 1790 |
| Eco47I | GGWCC | 4 cut(s) | 683, 1154, 1199, 1436 |
| Eco53kI | GAGCTC | 1 cut(s) | 1335 |
| Eco57I | CTGAAG | 2 cut(s) | 1005, 1554 |
| Eco91I | GGTNACC | 1 cut(s) | 1350 |
| EcoICRI | GAGCTC | 1 cut(s) | 1335 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1752 |
| EcoO109I | RGGNCCY | 1 cut(s) | 683 |
| EcoO65I | GGTNACC | 1 cut(s) | 1350 |
| EcoRII | CCWGG | 3 cut(s) | 247, 1171, 1731 |
| EcoRV | GATATC | 1 cut(s) | 1790 |
| EcoT14I | CCWWGG | 4 cut(s) | 172, 370, 716, 1402 |
| EcoT22I | ATGCAT | 1 cut(s) | 25 |
| EcoT38I | GRGCYC | 2 cut(s) | 1172, 1337 |
| EgeI | GGCGCC | 1 cut(s) | 418 |
| EheI | GGCGCC | 1 cut(s) | 418 |
| ErhI | CCWWGG | 4 cut(s) | 172, 370, 716, 1402 |
| FaqI | GGGAC | 2 cut(s) | 195, 1185 |
| FauI | CCCGC | 1 cut(s) | 1460 |
| FbaI | TGATCA | 3 cut(s) | 499, 1114, 1807 |
| FokI | GGATG | 1 cut(s) | 1781 |
| FriOI | GRGCYC | 2 cut(s) | 1172, 1337 |
| FspBI | CTAG | 2 cut(s) | 116, 1824 |
| GlaI | GCGC | 2 cut(s) | 418, 1539 |
| GsaI | CCCAGC | 1 cut(s) | 1628 |
| HaeII | RGCGCY | 1 cut(s) | 420 |
| HaeIII | GGCC | 2 cut(s) | 1228, 1623 |
| HapII | CCGG | 3 cut(s) | 290, 315, 1203 |
| HgaI | GACGC | 1 cut(s) | 438 |
| HhaI | GCGC | 2 cut(s) | 419, 1540 |
| Hin1I | GRCGYC | 1 cut(s) | 417 |
| Hin6I | GCGC | 2 cut(s) | 417, 1538 |
| HinP1I | GCGC | 2 cut(s) | 417, 1538 |
| HindIII | AAGCTT | 2 cut(s) | 302, 1593 |
| HinfI | GANTC | 9 cut(s) | 124, 233, 615, 721, 803, 812, 1176, 1253, 1407 |
| HpaII | CCGG | 3 cut(s) | 290, 315, 1203 |
| HphI | GGTGA | 6 cut(s) | 218, 920, 1124, 1295, 1344, 1621 |
| Hpy166II | GTNNAC | 2 cut(s) | 562, 1240 |
| Hpy188I | TCNGA | 6 cut(s) | 326, 514, 645, 734, 1794, 1807 |
| Hpy188III | TCNNGA | 4 cut(s) | 116, 1149, 1180, 1330 |
| Hpy8I | GTNNAC | 2 cut(s) | 562, 1240 |
| HpyAV | CCTTC | 6 cut(s) | 203, 266, 640, 848, 1563, 1759 |
| HpyCH4III | ACNGT | 5 cut(s) | 506, 730, 1055, 1105, 1609 |
| HpyCH4IV | ACGT | 2 cut(s) | 564, 1578 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 467, 476, 626, 635, 932, 1167 |
| HpyF3I | CTNAG | 8 cut(s) | 592, 667, 979, 1216, 1307, 1483, 1689, 1793 |
| HpySE526I | ACGT | 2 cut(s) | 564, 1578 |
| Hsp92I | GRCGYC | 1 cut(s) | 417 |
| HspAI | GCGC | 2 cut(s) | 417, 1538 |
| KasI | GGCGCC | 1 cut(s) | 416 |
| KpnI | GGTACC | 2 cut(s) | 378, 1661 |
| Ksp22I | TGATCA | 3 cut(s) | 499, 1114, 1807 |
| KspI | CCGCGG | 1 cut(s) | 1468 |
| Kzo9I | GATC | 6 cut(s) | 346, 499, 942, 1114, 1807, 1839 |
| LmnI | GCTCC | 2 cut(s) | 635, 1523 |
| LweI | GCATC | 2 cut(s) | 1044, 1759 |
| MabI | ACCWGGT | 2 cut(s) | 247, 1731 |
| MaeI | CTAG | 2 cut(s) | 116, 1824 |
| MaeII | ACGT | 2 cut(s) | 564, 1578 |
| MaeIII | GTNAC | 7 cut(s) | 518, 1132, 1350, 1378, 1579, 1609, 1819 |
| MalI | GATC | 6 cut(s) | 348, 501, 944, 1116, 1809, 1841 |
| MbiI | CCGCTC | 1 cut(s) | 110 |
| MboI | GATC | 6 cut(s) | 346, 499, 942, 1114, 1807, 1839 |
| MboII | GAAGA | 5 cut(s) | 906, 923, 968, 1732, 1834 |
| MfeI | CAATTG | 1 cut(s) | 1374 |
| MflI | RGATCY | 1 cut(s) | 346 |
| MhlI | GDGCHC | 3 cut(s) | 570, 1172, 1337 |
| MluCI | AATT | 7 cut(s) | 199, 216, 639, 888, 897, 1374, 1546 |
| Mly113I | GGCGCC | 1 cut(s) | 417 |
| MlyI | GAGTC | 2 cut(s) | 227, 1416 |
| MmeI | TCCRAC | 1 cut(s) | 1693 |
| Mph1103I | ATGCAT | 1 cut(s) | 25 |
| MseI | TTAA | 3 cut(s) | 1269, 1365, 1743 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1308, 1835 |
| MspA1I | CMGCKG | 6 cut(s) | 596, 663, 1358, 1442, 1467, 1493 |
| MspI | CCGG | 3 cut(s) | 290, 315, 1203 |
| MspR9I | CCNGG | 4 cut(s) | 249, 1173, 1203, 1733 |
| MunI | CAATTG | 1 cut(s) | 1374 |
| Mva1269I | GAATGC | 1 cut(s) | 1813 |
| MvaI | CCWGG | 3 cut(s) | 249, 1173, 1733 |
| MvnI | CGCG | 1 cut(s) | 1467 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 467, 476, 626, 635, 932, 1167 |
| NarI | GGCGCC | 1 cut(s) | 417 |
| NciI | CCSGG | 1 cut(s) | 1203 |
| NcoI | CCATGG | 2 cut(s) | 370, 716 |
| NdeII | GATC | 6 cut(s) | 346, 499, 942, 1114, 1807, 1839 |
| NmuCI | GTSAC | 6 cut(s) | 518, 1132, 1350, 1378, 1579, 1609 |
| NsiI | ATGCAT | 1 cut(s) | 25 |
| NspI | RCATGY | 1 cut(s) | 387 |
| OliI | CACNNNNGTG | 1 cut(s) | 1308 |
| PaqCI | CACCTGC | 3 cut(s) | 917, 1016, 1135 |
| PctI | GAATGC | 1 cut(s) | 1813 |
| PfeI | GAWTC | 7 cut(s) | 124, 615, 721, 803, 812, 1176, 1253 |
| PflFI | GACNNNGTC | 1 cut(s) | 1585 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 34, 178 |
| PleI | GAGTC | 2 cut(s) | 227, 1415 |
| PluTI | GGCGCC | 1 cut(s) | 420 |
| PpsI | GAGTC | 2 cut(s) | 227, 1415 |
| PpuMI | RGGWCCY | 1 cut(s) | 683 |
| PsiI | TTATAA | 1 cut(s) | 609 |
| Psp124BI | GAGCTC | 1 cut(s) | 1337 |
| Psp5II | RGGWCCY | 1 cut(s) | 683 |
| Psp6I | CCWGG | 3 cut(s) | 247, 1171, 1731 |
| PspEI | GGTNACC | 1 cut(s) | 1350 |
| PspFI | CCCAGC | 1 cut(s) | 1624 |
| PspGI | CCWGG | 3 cut(s) | 247, 1171, 1731 |
| PspPI | GGNCC | 5 cut(s) | 683, 1154, 1199, 1436, 1622 |
| PspPPI | RGGWCCY | 1 cut(s) | 683 |
| PstI | CTGCAG | 2 cut(s) | 1027, 1132 |
| PstNI | CAGNNNCTG | 1 cut(s) | 452 |
| PsuI | RGATCY | 1 cut(s) | 346 |
| PsyI | GACNNNGTC | 1 cut(s) | 1585 |
| PvuII | CAGCTG | 4 cut(s) | 596, 663, 1358, 1442 |
| RsaI | GTAC | 4 cut(s) | 376, 485, 1395, 1659 |
| RsaNI | GTAC | 4 cut(s) | 375, 484, 1394, 1658 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1308, 1835 |
| SacI | GAGCTC | 1 cut(s) | 1337 |
| SacII | CCGCGG | 1 cut(s) | 1468 |
| SaqAI | TTAA | 3 cut(s) | 1269, 1365, 1743 |
| Sau3AI | GATC | 6 cut(s) | 346, 499, 942, 1114, 1807, 1839 |
| Sau96I | GGNCC | 5 cut(s) | 683, 1154, 1199, 1436, 1622 |
| ScaI | AGTACT | 1 cut(s) | 1395 |
| SchI | GAGTC | 2 cut(s) | 227, 1416 |
| ScrFI | CCNGG | 4 cut(s) | 249, 1173, 1203, 1733 |
| SduI | GDGCHC | 3 cut(s) | 570, 1172, 1337 |
| SexAI | ACCWGGT | 2 cut(s) | 247, 1731 |
| SfaNI | GCATC | 2 cut(s) | 1044, 1759 |
| SfcI | CTRYAG | 4 cut(s) | 970, 1023, 1128, 1257 |
| SfoI | GGCGCC | 1 cut(s) | 418 |
| Sfr303I | CCGCGG | 1 cut(s) | 1468 |
| SgrBI | CCGCGG | 1 cut(s) | 1468 |
| SinI | GGWCC | 4 cut(s) | 683, 1154, 1199, 1436 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1308, 1835 |
| SmlI | CTYRAG | 4 cut(s) | 1328, 1389, 1515, 1590 |
| SmoI | CTYRAG | 4 cut(s) | 1328, 1389, 1515, 1590 |
| Sse9I | AATT | 7 cut(s) | 199, 216, 639, 888, 897, 1374, 1546 |
| SsiI | CCGC | 5 cut(s) | 108, 1465, 1467, 1493, 1853 |
| SspDI | GGCGCC | 1 cut(s) | 416 |
| SspMI | CTAG | 2 cut(s) | 116, 1824 |
| SstI | GAGCTC | 1 cut(s) | 1337 |
| StyD4I | CCNGG | 4 cut(s) | 247, 1171, 1201, 1731 |
| StyI | CCWWGG | 4 cut(s) | 172, 370, 716, 1402 |
| TaaI | ACNGT | 5 cut(s) | 506, 730, 1055, 1105, 1609 |
| TaiI | ACGT | 2 cut(s) | 567, 1581 |
| TasI | AATT | 7 cut(s) | 199, 216, 639, 888, 897, 1374, 1546 |
| TatI | WGTACW | 1 cut(s) | 1393 |
| TfiI | GAWTC | 7 cut(s) | 124, 615, 721, 803, 812, 1176, 1253 |
| Tru1I | TTAA | 3 cut(s) | 1269, 1365, 1743 |
| Tru9I | TTAA | 3 cut(s) | 1269, 1365, 1743 |
| TscAI | CASTG | 3 cut(s) | 547, 1108, 1462 |
| TseFI | GTSAC | 6 cut(s) | 518, 1132, 1350, 1378, 1579, 1609 |
| Tsp45I | GTSAC | 6 cut(s) | 518, 1132, 1350, 1378, 1579, 1609 |
| TspDTI | ATGAA | 7 cut(s) | 17, 285, 687, 1134, 1440, 1520, 1545 |
| TspRI | CASTG | 3 cut(s) | 547, 1108, 1462 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1585 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 34, 178 |
| VpaK11BI | GGWCC | 4 cut(s) | 683, 1154, 1199, 1436 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1752 |
| XbaI | TCTAGA | 1 cut(s) | 115 |
| XceI | RCATGY | 1 cut(s) | 387 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 38 |
| XspI | CTAG | 2 cut(s) | 116, 1824 |
| ZrmI | AGTACT | 1 cut(s) | 1395 |
| Zsp2I | ATGCAT | 1 cut(s) | 25 |