RchiOBHm_Chr6g0266941

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000302
Physical Location & Seq
Forward (+)
23451586 .. 23453880
2295 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23942

Sequence Viewer

Length: 2295 bp
ATGGAGAACAAAATGTGTGTAGTGATGCAGATCATATACCTTGGTTTTAGTTTCATGTTTTACATGTCACTAGCAGTTGCTGATGAAAAAGTGGCAGCATTGGAGCCGCTAGCCAAGACAAAGAGCATGCAAACTTATATAGTTTGGGTGGAAAAGCCAGTTAGCGATTTGATTGCACAATCTGAACATGAAGATTTAGGGAGTTGGTACCAGACATTTTTGCCCCAAACTATTGCAAACTCAAACCAGCTTGAGAAGCCACGAATGGTTTATGCATACCACAATGTGGCAACTGGCTTCGCAGCAAAGCTGACAGCGAAGGAAGTTGAAGAGATGGAAAAGAAAGATGGGTTTATCTCCGCTCATCCAGAACGAAATCTGCAATTGCACACAACTCATAGTCCTAACTTCTTGGGGCTGCAGCAAGGAATGGGACTCGATAAAGGGCCTAACTATGGTGAAGGTGTGATTATTGGAATTTTGGATAGTGGGATATTCCCAGACCATCCTTCATTTAGTGATGAAGGAGTACCACCTCCTCCAGCTAAATGGAAAGGCAAGTGTGATTTCAGCGGGACAGTCTGCAATAACAAGCTTATAGGTGCCAGAAGTTTTAATGGTGGAAAAACTACTGGAGGCCCTCCAGTTGACGATGAAGGCCATGGAACTCACACGTCAAGCACAGCTGGAGGAAATTTTGTAAAAGGTGCCAGCGTGTTTGGAATGGCCAACGGCACAGCAACTGGCATGGCACCTCATGCTCACTTGGCAATGTACAAAGTATGCTCAGACGAGGGTTGTTCTGAATCAGACATTATAGCTGCTATGGACACTGCTGTTGATGATGGCGTGGATGTGCTATCCCTTTCAATCGGTGGTGGCTCAGCTTATTTTTATGCTGATGGAATTGCAGTCGGCGCATTTGGAGCAATTCAGAAGGGAATCTTTGTCAGCTGTTCAGCTGGAAATGCTGGCCCTGACTATATGACTTTATCCAATGAAGCTCCATGGATTCTCACGGTTGGAGCAAGCACCATTGACAGAAACATAAGAGCAACAGCAAAGCTTGGAAATGGGCAAGAATATGACGGGGAGTCAGTATTCCAACCTAAAGATTTTAGTTCAAAACAGTTAGCTCTTGTTTATGCAGGTGCACATAGCAATGATTCATCAGCTCTTTGCGATGTAGGGTCCCTTAAATATGTTGAAGGGAAAGTAGTGGTGTGTGAGGTAGGTGGAGGAACTGGAAGAATTGCAAAAGGGGTAGAAGTGAAAAGAGCTGGTGGTGTTGCCATGATTCTTGTCAACCCAGACTTCGCTGGCTATACCACCATAGCAGACGCTCACGTGCTTCCGGCAACACATGTGAGTTACTCTGCAGGGGTGAGCATCAAAACCTATATAAATTCAACCTCAACTCCTACAGCGACAATCTTATTCAAAGGCACTGTCATCGGTGATCAGCTTGCTCCCAAGGTTGCTTTCTTTTCATCAAGAGGACCAAGCCTGGCAAGCCCTGCAATTTTGAAACCTGACATTATTGGTCCCGGTGTGAGCATCCTAGCTGCGTGGCCTTTTTCGGTGGATAATGCTTTAGAATCTAAGGCCACATTTGACATTATTTCAGGTACTTCAATGTCATGCCCTCACCTAAGTGGCATTGCAGCCTTGCTCAAGAGCTCACACCCTGACTGGTCACCTGCTGCCATTAAGTCTGCAATGATGACAACTGCTGAAGTAAACAACCTCGCTGGCTCGGCCATTCTTGATCAAACACTTCTTGCAGCAGACCTCTTTGCCATTGGCGCAGGCCATGTTAACCCTTCAAAAGCAAATGACCCTGGGCTCATCTACGACATACAACCAGAGGACTACATTCCTTACTTGTGTGGTTTGAATTACACAAGCAAACAGATATCGGCAATCACCCAACAAAAAGTGTCGTGCTCTAAAGTAGGAGTCATACCTGAAGCACAGCTAAACTATCCCACATTTTGTATCTATATAGAGTCTGGTGGTGAGCCTCAGAATTACACAAGAACCTTGACCAATGTTGGCCCAGCTAATTCAACTTACAAATTGGCTCCCCTGAGACTACATAAAATGGACATCACTGTGCTACCTGAGGTGCTTACATTTACAGAGGTTAACCAAAAATTGACATACCGTGTGGTGTTTGCCGCACAAGATGGAGCTGGGAAAGATGGTATACCATTTTCTCAGGGATATTTGAGTTGGGTGTCTCGTCAGCATTCTGTTAACAGCCAAATATCTGTGGTCTTTGACTATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

764

Amino Acids

80.93

Weight (kDa)

5.42

Isoelectric Point (pI)

31.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 45 - 130 7.2e-11 Peptidase inhibitor I9
Peptidase_S8 PF00082 153 - 581 3.3e-46 Subtilase family
PA PF02225 380 - 463 8.6e-10 PA domain
fn3_6 PF17766 659 - 759 3e-21 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1139, 1708
Acc36I ACCTGC 2 cut(s) 1139, 1708
Acc65I GGTACC 1 cut(s) 207
AccB1I GGYRCC 4 cut(s) 207, 602, 707, 751
AccB7I CCANNNNNTGG 1 cut(s) 286
AccBSI CCGCTC 1 cut(s) 362
AccI GTMKAC 1 cut(s) 2209
AciI CCGC 4 cut(s) 107, 360, 573, 2181
AcoI YGGCCR 2 cut(s) 726, 1758
AcsI RAATTY 3 cut(s) 477, 694, 1405
AcuI CTGAAG 2 cut(s) 1755, 1989
AcvI CACGTG 1 cut(s) 1348
AdeI CACNNNGTG 1 cut(s) 286
AfaI GTAC 4 cut(s) 209, 531, 776, 1630
AfiI CCNNNNNNNGG 2 cut(s) 286, 455
AflIII ACRYGT 3 cut(s) 63, 672, 1363
AhdI GACNNNNNGTC 1 cut(s) 1093
AjiI CACGTC 1 cut(s) 675
AjnI CCWGG 2 cut(s) 1506, 1840
AjuI GAANNNNNNNTTGG 2 cut(s) 1466, 1498
AleI CACNNNNGTG 1 cut(s) 1653
Alw21I GWGCWC 3 cut(s) 1156, 1682, 1949
Alw26I GTCTC 2 cut(s) 2086, 2247
Alw44I GTGCAC 1 cut(s) 1152
AlwNI CAGNNNCTG 2 cut(s) 80, 743
ApaLI GTGCAC 1 cut(s) 1152
ApeKI GCWGC 9 cut(s) 95, 302, 418, 421, 821, 1565, 1664, 1703, 1784
ApoI RAATTY 3 cut(s) 477, 694, 1405
ArsI GACNNNNNNTTYG 2 cut(s) 1434, 1466
Asp718I GGTACC 1 cut(s) 207
AspLEI GCGC 2 cut(s) 920, 1808
AspS9I GGNCC 7 cut(s) 446, 638, 974, 1191, 1499, 1544, 2057
AsuC2I CCSGG 1 cut(s) 1548
AsuHPI GGTGA 7 cut(s) 470, 1396, 1469, 1640, 1689, 1918, 2030
AsuNHI GCTAGC 1 cut(s) 109
AvaII GGWCC 3 cut(s) 1191, 1499, 1544
AxyI CCTNAGG 1 cut(s) 2124
BaeGI GKGCMC 1 cut(s) 1156
BalI TGGCCA 1 cut(s) 728
BanI GGYRCC 4 cut(s) 207, 602, 707, 751
BanII GRGCYC 2 cut(s) 1682, 1848
BbrPI CACGTG 1 cut(s) 1348
Bbv12I GWGCWC 3 cut(s) 1156, 1682, 1949
BbvI GCAGC 9 cut(s) 107, 314, 405, 433, 808, 1552, 1676, 1690, 1796
BccI CCATC 7 cut(s) 328, 341, 513, 839, 896, 2183, 2198
BceAI ACGGC 1 cut(s) 748
BcgI CGANNNNNNTGC 4 cut(s) 155, 189, 1435, 1469
BciT130I CCWGG 2 cut(s) 1508, 1842
BclI TGATCA 2 cut(s) 1459, 1768
BcnI CCSGG 1 cut(s) 1548
BcoDI GTCTC 2 cut(s) 2086, 2247
BfaI CTAG 3 cut(s) 71, 110, 1562
BfmI CTRYAG 3 cut(s) 419, 1377, 1422
BfuAI ACCTGC 2 cut(s) 1139, 1708
BlpI GCTNAGC 1 cut(s) 883
Bme1390I CCNGG 3 cut(s) 1508, 1548, 1842
Bme18I GGWCC 3 cut(s) 1191, 1499, 1544
BmeRI GACNNNNNGTC 1 cut(s) 1093
BmgBI CACGTC 1 cut(s) 675
BmgT120I GGNCC 7 cut(s) 446, 638, 974, 1191, 1499, 1544, 2057
BmiI GGNNCC 9 cut(s) 105, 209, 604, 709, 753, 1192, 1193, 1546, 2085
BmrFI CCNGG 3 cut(s) 1508, 1548, 1842
BmsI GCATC 3 cut(s) 15, 1398, 1566
BmtI GCTAGC 1 cut(s) 113
BpmI CTGGAG 4 cut(s) 525, 627, 654, 708
Bpu1102I GCTNAGC 1 cut(s) 883
BpuEI CTTGAG 2 cut(s) 272, 1658
BpuMI CCSGG 1 cut(s) 1548
BsaAI YACGTR 1 cut(s) 1348
BsaBI GATNNNNATC 1 cut(s) 29
BsaJI CCNNGG 6 cut(s) 40, 661, 1007, 1473, 1840, 1841
BsaXI ACNNNNNCTCC 2 cut(s) 1402, 1432
Bsc4I CCNNNNNNNGG 2 cut(s) 286, 455
Bse1I ACTGG 7 cut(s) 158, 298, 637, 644, 748, 1249, 1697
Bse21I CCTNAGG 1 cut(s) 2124
Bse3DI GCAATG 4 cut(s) 777, 1168, 1659, 1725
Bse8I GATNNNNATC 1 cut(s) 29
BseBI CCWGG 2 cut(s) 1508, 1842
BseDI CCNNGG 6 cut(s) 40, 661, 1007, 1473, 1840, 1841
BseGI GGATG 4 cut(s) 364, 505, 859, 1557
BseJI GATNNNNATC 1 cut(s) 29
BseLI CCNNNNNNNGG 2 cut(s) 286, 455
BseMI GCAATG 4 cut(s) 777, 1168, 1659, 1725
BseMII CTCAG 6 cut(s) 801, 897, 2039, 2081, 2115, 2234
BseNI ACTGG 7 cut(s) 158, 298, 637, 644, 748, 1249, 1697
BseRI GAGGAG 1 cut(s) 528
BseSI GKGCMC 1 cut(s) 1156
BseXI GCAGC 9 cut(s) 107, 314, 405, 433, 808, 1552, 1676, 1690, 1796
BseYI CCCAGC 2 cut(s) 2059, 2195
BshNI GGYRCC 4 cut(s) 207, 602, 707, 751
BsiHKAI GWGCWC 3 cut(s) 1156, 1682, 1949
BsiSI CCGG 2 cut(s) 1355, 1548
BslFI GGGAC 4 cut(s) 447, 589, 1177, 1530
BslI CCNNNNNNNGG 2 cut(s) 286, 455
BsmAI GTCTC 2 cut(s) 2086, 2247
BsmFI GGGAC 4 cut(s) 447, 589, 1177, 1530
BsmI GAATGC 1 cut(s) 2251
Bsp1286I GDGCHC 4 cut(s) 1156, 1682, 1848, 1949
Bsp1407I TGTACA 1 cut(s) 774
Bsp143I GATC 3 cut(s) 30, 1459, 1768
Bsp1720I GCTNAGC 1 cut(s) 883
Bsp19I CCATGG 2 cut(s) 661, 1007
BspACI CCGC 4 cut(s) 107, 360, 573, 2181
BspCNI CTCAG 6 cut(s) 800, 896, 2038, 2082, 2116, 2233
BspLI GGNNCC 9 cut(s) 105, 209, 604, 709, 753, 1192, 1193, 1546, 2085
BspMAI CTGCAG 2 cut(s) 423, 1381
BspMI ACCTGC 2 cut(s) 1139, 1708
BspOI GCTAGC 1 cut(s) 113
BspT107I GGYRCC 4 cut(s) 207, 602, 707, 751
BsrBI CCGCTC 1 cut(s) 362
BsrDI GCAATG 4 cut(s) 777, 1168, 1659, 1725
BsrGI TGTACA 1 cut(s) 774
BsrI ACTGG 7 cut(s) 158, 298, 637, 644, 748, 1249, 1697
BssECI CCNNGG 6 cut(s) 40, 661, 1007, 1473, 1840, 1841
BssMI GATC 3 cut(s) 30, 1459, 1768
BssNAI GTATAC 1 cut(s) 2210
BssT1I CCWWGG 4 cut(s) 40, 661, 1007, 1473
Bst1107I GTATAC 1 cut(s) 2210
Bst2UI CCWGG 2 cut(s) 1508, 1842
Bst4CI ACNGT 6 cut(s) 580, 1021, 1131, 1450, 2116, 2168
Bst6I CTCTTC 1 cut(s) 324
BstAPI GCANNNNNTGC 2 cut(s) 758, 1517
BstAUI TGTACA 1 cut(s) 774
BstBAI YACGTR 1 cut(s) 1348
BstDEI CTNAG 8 cut(s) 787, 883, 1602, 1652, 2025, 2090, 2124, 2220
BstDSI CCRYGG 2 cut(s) 661, 1007
BstEII GGTNACC 1 cut(s) 1695
BstF5I GGATG 4 cut(s) 364, 505, 859, 1557
BstHHI GCGC 2 cut(s) 920, 1808
BstKTI GATC 3 cut(s) 33, 1462, 1771
BstMAI GTCTC 2 cut(s) 2086, 2247
BstMBI GATC 3 cut(s) 30, 1459, 1768
BstNI CCWGG 2 cut(s) 1508, 1842
BstNSI RCATGY 3 cut(s) 67, 130, 1367
BstPI GGTNACC 1 cut(s) 1695
BstSCI CCNGG 3 cut(s) 1506, 1546, 1840
BstSFI CTRYAG 3 cut(s) 419, 1377, 1422
BstSLI GKGCMC 1 cut(s) 1156
BstV1I GCAGC 9 cut(s) 107, 314, 405, 433, 808, 1552, 1676, 1690, 1796
BstXI CCANNNNNNTGG 1 cut(s) 549
BstZ17I GTATAC 1 cut(s) 2210
Bsu36I CCTNAGG 1 cut(s) 2124
BtgI CCRYGG 2 cut(s) 661, 1007
BtgZI GCGATG 1 cut(s) 1197
BtrI CACGTC 1 cut(s) 675
BtsCI GGATG 4 cut(s) 364, 505, 859, 1557
BtsI GCAGTG 1 cut(s) 831
BtsIMutI CAGTG 3 cut(s) 831, 1446, 2112
BveI ACCTGC 2 cut(s) 1139, 1708
CaiI CAGNNNCTG 2 cut(s) 80, 743
CfoI GCGC 2 cut(s) 920, 1808
Cfr13I GGNCC 7 cut(s) 446, 638, 974, 1191, 1499, 1544, 2057
CseI GACGC 1 cut(s) 1349
Csp6I GTAC 4 cut(s) 208, 530, 775, 1629
CviQI GTAC 4 cut(s) 208, 530, 775, 1629
DdeI CTNAG 8 cut(s) 787, 883, 1602, 1652, 2025, 2090, 2124, 2220
DpnI GATC 3 cut(s) 32, 1461, 1770
DpnII GATC 3 cut(s) 30, 1459, 1768
DraIII CACNNNGTG 1 cut(s) 286
DriI GACNNNNNGTC 1 cut(s) 1093
EaeI YGGCCR 2 cut(s) 726, 1758
Eam1104I CTCTTC 1 cut(s) 324
Eam1105I GACNNNNNGTC 1 cut(s) 1093
EarI CTCTTC 1 cut(s) 324
Ecl136II GAGCTC 1 cut(s) 1680
Eco130I CCWWGG 4 cut(s) 40, 661, 1007, 1473
Eco24I GRGCYC 2 cut(s) 1682, 1848
Eco32I GATATC 1 cut(s) 1917
Eco47I GGWCC 3 cut(s) 1191, 1499, 1544
Eco53kI GAGCTC 1 cut(s) 1680
Eco57I CTGAAG 2 cut(s) 1755, 1989
Eco72I CACGTG 1 cut(s) 1348
Eco81I CCTNAGG 1 cut(s) 2124
Eco91I GGTNACC 1 cut(s) 1695
EcoICRI GAGCTC 1 cut(s) 1680
EcoO109I RGGNCCY 3 cut(s) 446, 638, 1191
EcoO65I GGTNACC 1 cut(s) 1695
EcoRII CCWGG 2 cut(s) 1506, 1840
EcoRV GATATC 1 cut(s) 1917
EcoT14I CCWWGG 4 cut(s) 40, 661, 1007, 1473
EcoT22I ATGCAT 1 cut(s) 277
EcoT38I GRGCYC 2 cut(s) 1682, 1848
ErhI CCWWGG 4 cut(s) 40, 661, 1007, 1473
FalI AAGNNNNNCTT 2 cut(s) 929, 961
FaqI GGGAC 4 cut(s) 447, 589, 1177, 1530
FauI CCCGC 1 cut(s) 566
FbaI TGATCA 2 cut(s) 1459, 1768
FblI GTMKAC 1 cut(s) 2209
FokI GGATG 4 cut(s) 351, 492, 866, 1544
FriOI GRGCYC 2 cut(s) 1682, 1848
FspBI CTAG 3 cut(s) 71, 110, 1562
GlaI GCGC 2 cut(s) 919, 1807
GsaI CCCAGC 2 cut(s) 2063, 2199
GsuI CTGGAG 4 cut(s) 525, 627, 654, 708
HapII CCGG 2 cut(s) 1355, 1548
HgaI GACGC 1 cut(s) 1349
HhaI GCGC 2 cut(s) 920, 1808
Hin6I GCGC 2 cut(s) 918, 1806
HinP1I GCGC 2 cut(s) 918, 1806
HincII GTYRAC 5 cut(s) 649, 1306, 1819, 2149, 2260
HindII GTYRAC 5 cut(s) 649, 1306, 1819, 2149, 2260
HindIII AAGCTT 2 cut(s) 593, 1064
HpaI GTTAAC 3 cut(s) 1819, 2149, 2260
HpaII CCGG 2 cut(s) 1355, 1548
HphI GGTGA 7 cut(s) 470, 1396, 1469, 1640, 1689, 1918, 2030
Hpy166II GTNNAC 8 cut(s) 649, 1154, 1306, 1741, 1819, 2149, 2210, 2260
Hpy188I TCNGA 6 cut(s) 184, 790, 805, 811, 936, 2028
Hpy188III TCNNGA 4 cut(s) 368, 1494, 1675, 1766
Hpy8I GTNNAC 8 cut(s) 649, 1154, 1306, 1741, 1819, 2149, 2210, 2260
HpyAV CCTTC 8 cut(s) 313, 455, 518, 519, 650, 931, 1202, 1833
HpyCH4III ACNGT 6 cut(s) 580, 1021, 1131, 1450, 2116, 2168
HpyCH4IV ACGT 2 cut(s) 674, 1347
HpyF3I CTNAG 8 cut(s) 787, 883, 1602, 1652, 2025, 2090, 2124, 2220
HpySE526I ACGT 2 cut(s) 674, 1347
HspAI GCGC 2 cut(s) 918, 1806
KflI GGGWCCC 1 cut(s) 1191
KpnI GGTACC 1 cut(s) 211
Ksp22I TGATCA 2 cut(s) 1459, 1768
KspAI GTTAAC 3 cut(s) 1819, 2149, 2260
Kzo9I GATC 3 cut(s) 30, 1459, 1768
LmnI GCTCC 7 cut(s) 103, 926, 1009, 1025, 1474, 2089, 2192
Lsp1109I GCAGC 9 cut(s) 107, 314, 405, 433, 808, 1552, 1676, 1690, 1796
LweI GCATC 3 cut(s) 15, 1398, 1566
MaeI CTAG 3 cut(s) 71, 110, 1562
MaeII ACGT 2 cut(s) 674, 1347
MaeIII GTNAC 3 cut(s) 66, 1370, 1695
MalI GATC 3 cut(s) 32, 1461, 1770
MbiI CCGCTC 1 cut(s) 362
MboI GATC 3 cut(s) 30, 1459, 1768
MboII GAAGA 3 cut(s) 203, 341, 1260
MfeI CAATTG 1 cut(s) 383
MhlI GDGCHC 4 cut(s) 1156, 1682, 1848, 1949
MlsI TGGCCA 1 cut(s) 728
MluNI TGGCCA 1 cut(s) 728
MlyI GAGTC 4 cut(s) 429, 1103, 1968, 2018
MmeI TCCRAC 2 cut(s) 1003, 1129
Mox20I TGGCCA 1 cut(s) 728
Mph1103I ATGCAT 1 cut(s) 277
MscI TGGCCA 1 cut(s) 728
MseI TTAA 6 cut(s) 615, 1197, 1710, 1818, 2148, 2259
MslI CAYNNNNRTG 3 cut(s) 1161, 1653, 2114
Msp20I TGGCCA 1 cut(s) 728
MspA1I CMGCKG 4 cut(s) 573, 686, 954, 962
MspI CCGG 2 cut(s) 1355, 1548
MspR9I CCNGG 3 cut(s) 1508, 1548, 1842
MunI CAATTG 1 cut(s) 383
Mva1269I GAATGC 1 cut(s) 2251
MvaI CCWGG 2 cut(s) 1508, 1842
NciI CCSGG 1 cut(s) 1548
NcoI CCATGG 2 cut(s) 661, 1007
NdeII GATC 3 cut(s) 30, 1459, 1768
NheI GCTAGC 1 cut(s) 109
NlaIV GGNNCC 9 cut(s) 105, 209, 604, 709, 753, 1192, 1193, 1546, 2085
NmeAIII GCCGAG 1 cut(s) 1736
NmuCI GTSAC 2 cut(s) 66, 1695
NsiI ATGCAT 1 cut(s) 277
NspI RCATGY 3 cut(s) 67, 130, 1367
OliI CACNNNNGTG 1 cut(s) 1653
PaeI GCATGC 1 cut(s) 130
PaqCI CACCTGC 2 cut(s) 1139, 1708
PasI CCCWGGG 1 cut(s) 1841
PciI ACATGT 2 cut(s) 63, 1363
PctI GAATGC 1 cut(s) 2251
PfeI GAWTC 6 cut(s) 806, 942, 1012, 1166, 1297, 1598
PflMI CCANNNNNTGG 1 cut(s) 286
PleI GAGTC 4 cut(s) 429, 1102, 1967, 2017
PmaCI CACGTG 1 cut(s) 1348
PmlI CACGTG 1 cut(s) 1348
PpsI GAGTC 4 cut(s) 429, 1102, 1967, 2017
Ppu21I YACGTR 1 cut(s) 1348
PpuMI RGGWCCY 1 cut(s) 1191
PscI ACATGT 2 cut(s) 63, 1363
Psp124BI GAGCTC 1 cut(s) 1682
Psp5II RGGWCCY 1 cut(s) 1191
Psp6I CCWGG 2 cut(s) 1506, 1840
PspCI CACGTG 1 cut(s) 1348
PspEI GGTNACC 1 cut(s) 1695
PspFI CCCAGC 2 cut(s) 2059, 2195
PspGI CCWGG 2 cut(s) 1506, 1840
PspN4I GGNNCC 9 cut(s) 105, 209, 604, 709, 753, 1192, 1193, 1546, 2085
PspPI GGNCC 7 cut(s) 446, 638, 974, 1191, 1499, 1544, 2057
PspPPI RGGWCCY 1 cut(s) 1191
PstI CTGCAG 2 cut(s) 423, 1381
PstNI CAGNNNCTG 2 cut(s) 80, 743
PvuII CAGCTG 3 cut(s) 686, 954, 962
RsaI GTAC 4 cut(s) 209, 531, 776, 1630
RsaNI GTAC 4 cut(s) 208, 530, 775, 1629
RseI CAYNNNNRTG 3 cut(s) 1161, 1653, 2114
SacI GAGCTC 1 cut(s) 1682
SaqAI TTAA 6 cut(s) 615, 1197, 1710, 1818, 2148, 2259
Sau3AI GATC 3 cut(s) 30, 1459, 1768
Sau96I GGNCC 7 cut(s) 446, 638, 974, 1191, 1499, 1544, 2057
SchI GAGTC 4 cut(s) 429, 1103, 1968, 2018
ScrFI CCNGG 3 cut(s) 1508, 1548, 1842
SduI GDGCHC 4 cut(s) 1156, 1682, 1848, 1949
SfaNI GCATC 3 cut(s) 15, 1398, 1566
SfcI CTRYAG 3 cut(s) 419, 1377, 1422
SinI GGWCC 3 cut(s) 1191, 1499, 1544
SmiMI CAYNNNNRTG 3 cut(s) 1161, 1653, 2114
SmlI CTYRAG 2 cut(s) 251, 1673
SmoI CTYRAG 2 cut(s) 251, 1673
SphI GCATGC 1 cut(s) 130
SsiI CCGC 4 cut(s) 107, 360, 573, 2181
SspMI CTAG 3 cut(s) 71, 110, 1562
SstI GAGCTC 1 cut(s) 1682
StyD4I CCNGG 3 cut(s) 1506, 1546, 1840
StyI CCWWGG 4 cut(s) 40, 661, 1007, 1473
TaaI ACNGT 6 cut(s) 580, 1021, 1131, 1450, 2116, 2168
TaiI ACGT 2 cut(s) 677, 1350
TaqI TCGA 1 cut(s) 438
TatI WGTACW 1 cut(s) 774
TauI GCSGC 2 cut(s) 109, 2183
TfiI GAWTC 6 cut(s) 806, 942, 1012, 1166, 1297, 1598
Tru1I TTAA 6 cut(s) 615, 1197, 1710, 1818, 2148, 2259
Tru9I TTAA 6 cut(s) 615, 1197, 1710, 1818, 2148, 2259
TscAI CASTG 3 cut(s) 838, 1453, 2119
TseFI GTSAC 2 cut(s) 66, 1695
TseI GCWGC 9 cut(s) 95, 302, 418, 421, 821, 1565, 1664, 1703, 1784
Tsp45I GTSAC 2 cut(s) 66, 1695
TspDTI ATGAA 9 cut(s) 43, 99, 204, 501, 537, 669, 1014, 1158, 1479
TspRI CASTG 3 cut(s) 838, 1453, 2119
Van91I CCANNNNNTGG 1 cut(s) 286
VneI GTGCAC 1 cut(s) 1152
VpaK11BI GGWCC 3 cut(s) 1191, 1499, 1544
XapI RAATTY 3 cut(s) 477, 694, 1405
XceI RCATGY 3 cut(s) 67, 130, 1367
XmiI GTMKAC 1 cut(s) 2209
XspI CTAG 3 cut(s) 71, 110, 1562
Zsp2I ATGCAT 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.