Rh1DG180300

Subtilisin-like serine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000302
Physical Location & Seq
Reverse (-)
36471424 .. 36473642
2219 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG180300.1

Sequence Viewer

Length: 1614 bp
ATGGTTCACTCATACCTCAATGTGGCCACCGACTTTGCAGCAAAACTGACACCAGAGGAAGTGAAAGCAATGGAAAAGAAAGATGGGTTCGTGCATGCTCATCCAGAGAGGTTTCTGCCTTTGCACACTACTCATAGTCCAAACTTCCTGGGGCTGCAACAAGGGTTCGGACTTTGGAAAAGGACTAATTACGGTGAAGGTTTTGCTTCCTCAGTTCCTTTCCATCAAGATGGGGTTGCTATCGGCGCGTTTGGAGCAATTCGAAAGGGAATTTTTGTCAGCTGTTTAGCTGGAAACTCTGGCCCTTTCTATAGTTCCATGTCAAACGAGGCCCCTTGGATTCTCACCGTTGGAGCAAGCACCATTGATAGAAAAATAAGCTCAACAGCACAGCTTGGAGATTCTCCAAAATATTTTTACTCTGGTGAATCTTTATTCGATCCTAAAAATTTTAGCACTTCACCATTGCTACCTCTTGTTTATCCAGGTGCAAATCCCAATAACCAAACTGAGTTCTGTGCCTTGGGATCTCTTGTAAATGTTGAAGGGAAAGTAGTGGTGTGTGAGAGAGGTGGAGGAGTGGGAAGAACTGCCAAAGGGGAAGAAGTGAAAAGAGCAGGAGGTGCTGCCATGATTCTAGTGAACCAAATCACTGATGACTATAGCACCTTAGCTGATGCTCATGTACTTCCAGCAACAAATGTTGGTTTTCAAGCAGGTTTGAGCATCAAAACCTATATAAACTCAACAACTATAGCCACAGTCTATTCCCCACGCACTGAAATCGGAGATGCGCTTGCTCCAATTGTTGCTTCTTTTTCATCAAGAGGACCGAGCATAGCTAGCCCCGGAATTTTAAAACCTGACATAATTGGTCTTGGTGTTAACATCCTTGTTGCATGGCCTGTTTCGGTGGATAATGTCACAACATTTGACATTCTTTCAGGTACCTCAGTGTCATGCCCACACCTTAGTGGCATTGCAGCCTTGATCAAGAGTTCACACCCGGACTGGTCACCTGCTGCCATCAAATCCGCCATTATGACAACCGCTGATCTGCTAAACCTCGAAGGCACGCCAATTGTTGATAGATCAACTATCCCAGCAGACATCTATAGCATTGGCTCAGGCCATGTCAACCTTTCAAAAGCCAATGACCCGGGGCTCATCTATGACATAAAACCAGAAGATTACATTCCTTACTTGTGTGGTCTGAACTACACAGACAATCAAATAGAGATGATCACCCAACAAAGAGTGGACTGCTCTGAAGTAGGAGTCACAGCTGAAGCGCAGCTAAACTATCCTTCATTTTCTATCACAATAGGGTCCTATCAAACTCAGAATTACACAAGAACTGTGACAAATGTTGGCCCAGCTAATTCAACCTACAAATCGAAGATTTTGAAACCCCAGAAATGGGGTGTGAGTGTGAACATGGGTGTGACTCCTTCGGTGCTTATATTCATAGAGGTCAACCAGACCATTACATACAATGTGGAGTTCTCCACACAACTAGCTGGGCAAGATGGTTCATTTTCACAAGGATATTTGAGATGGCTTTCTGACATGTATTCTGTATACAGCCCCATAGCTGTCATCTTTCACTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

537

Amino Acids

57.54

Weight (kDa)

5.67

Isoelectric Point (pI)

33.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 80 - 353 1.4e-17 Subtilase family
PA PF02225 158 - 242 1.5e-11 PA domain
fn3_6 PF17766 432 - 533 9.4e-22 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1027
Acc36I ACCTGC 2 cut(s) 707, 1027
Acc65I GGTACC 1 cut(s) 947
AccB1I GGYRCC 1 cut(s) 947
AccI GTMKAC 1 cut(s) 1581
AccII CGCG 1 cut(s) 248
AciI CCGC 2 cut(s) 1035, 1050
AclWI GGATC 2 cut(s) 434, 535
AcoI YGGCCR 1 cut(s) 24
AcsI RAATTY 3 cut(s) 270, 448, 852
AcuI CTGAAG 2 cut(s) 1290, 1308
AfaI GTAC 2 cut(s) 687, 949
AfiI CCNNNNNNNGG 3 cut(s) 22, 1419, 1420
AflIII ACRYGT 1 cut(s) 1569
AgsI TTSAA 5 cut(s) 545, 713, 1146, 1386, 1408
AjnI CCWGG 2 cut(s) 147, 484
AjuI GAANNNNNNNTTGG 2 cut(s) 796, 828
AleI CACNNNNGTG 1 cut(s) 972
AlwI GGATC 2 cut(s) 434, 535
Ama87I CYCGRG 1 cut(s) 1159
AoxI GGCC 6 cut(s) 24, 301, 330, 902, 1129, 1372
ApeKI GCWGC 6 cut(s) 38, 154, 626, 983, 1022, 1294
ApoI RAATTY 3 cut(s) 270, 448, 852
Asp718I GGTACC 1 cut(s) 947
AspLEI GCGC 3 cut(s) 248, 796, 1294
AspS9I GGNCC 5 cut(s) 302, 331, 830, 1329, 1373
AsuC2I CCSGG 4 cut(s) 849, 1007, 1160, 1161
AsuHPI GGTGA 6 cut(s) 206, 337, 437, 453, 1008, 1237
AsuII TTCGAA 1 cut(s) 262
AsuNHI GCTAGC 1 cut(s) 842
AvaI CYCGRG 1 cut(s) 1159
AvaII GGWCC 2 cut(s) 830, 1329
BaeI ACNNNNGTAYC 2 cut(s) 939, 972
BalI TGGCCA 1 cut(s) 26
BanI GGYRCC 1 cut(s) 947
BanII GRGCYC 1 cut(s) 1167
BbvI GCAGC 6 cut(s) 50, 141, 613, 995, 1009, 1306
BccI CCATC 6 cut(s) 77, 224, 231, 1034, 1523, 1551
BcgI CGANNNNNNTGC 2 cut(s) 766, 800
BciT130I CCWGG 2 cut(s) 149, 486
BclI TGATCA 2 cut(s) 990, 1242
BcnI CCSGG 4 cut(s) 849, 1007, 1160, 1161
BfaI CTAG 3 cut(s) 638, 843, 1517
BfmI CTRYAG 4 cut(s) 310, 661, 753, 1114
BfuAI ACCTGC 2 cut(s) 707, 1027
BisI GCNGC 6 cut(s) 39, 155, 627, 984, 1023, 1295
BlsI GCNGC 6 cut(s) 40, 156, 628, 985, 1024, 1296
Bme1390I CCNGG 6 cut(s) 149, 486, 849, 1007, 1160, 1161
Bme18I GGWCC 2 cut(s) 830, 1329
BmeT110I CYCGRG 1 cut(s) 1159
BmgT120I GGNCC 5 cut(s) 302, 331, 830, 1329, 1373
BmiI GGNNCC 3 cut(s) 333, 949, 1330
BmrFI CCNGG 6 cut(s) 149, 486, 849, 1007, 1160, 1161
BmsI GCATC 3 cut(s) 667, 735, 781
BmtI GCTAGC 1 cut(s) 846
Bpu10I CCTNAGC 2 cut(s) 670, 1126
Bpu14I TTCGAA 1 cut(s) 262
BpuMI CCSGG 4 cut(s) 849, 1007, 1160, 1161
BsaJI CCNNGG 6 cut(s) 148, 335, 522, 847, 1159, 1160
Bsc4I CCNNNNNNNGG 3 cut(s) 22, 1419, 1420
Bse1I ACTGG 1 cut(s) 1016
Bse3DI GCAATG 3 cut(s) 75, 464, 978
BseBI CCWGG 2 cut(s) 149, 486
BseDI CCNNGG 6 cut(s) 148, 335, 522, 847, 1159, 1160
BseGI GGATG 2 cut(s) 100, 888
BseLI CCNNNNNNNGG 3 cut(s) 22, 1419, 1420
BseMI GCAATG 3 cut(s) 75, 464, 978
BseMII CTCAG 5 cut(s) 225, 501, 966, 1140, 1355
BseNI ACTGG 1 cut(s) 1016
BseRI GAGGAG 1 cut(s) 591
BseXI GCAGC 6 cut(s) 50, 141, 613, 995, 1009, 1306
BseYI CCCAGC 3 cut(s) 1102, 1375, 1520
Bsh1236I CGCG 1 cut(s) 248
BshFI GGCC 6 cut(s) 26, 303, 332, 904, 1131, 1374
BshNI GGYRCC 1 cut(s) 947
BsiHKCI CYCGRG 1 cut(s) 1159
BsiSI CCGG 3 cut(s) 849, 1007, 1160
BslI CCNNNNNNNGG 3 cut(s) 22, 1419, 1420
BsnI GGCC 6 cut(s) 26, 303, 332, 904, 1131, 1374
BsoBI CYCGRG 1 cut(s) 1159
Bsp119I TTCGAA 1 cut(s) 262
Bsp1286I GDGCHC 1 cut(s) 1167
Bsp143I GATC 6 cut(s) 439, 527, 990, 1054, 1091, 1242
BspACI CCGC 2 cut(s) 1035, 1050
BspANI GGCC 6 cut(s) 26, 303, 332, 904, 1131, 1374
BspCNI CTCAG 5 cut(s) 224, 502, 965, 1139, 1354
BspFNI CGCG 1 cut(s) 248
BspLI GGNNCC 3 cut(s) 333, 949, 1330
BspMI ACCTGC 2 cut(s) 707, 1027
BspOI GCTAGC 1 cut(s) 846
BspPI GGATC 2 cut(s) 434, 535
BspT104I TTCGAA 1 cut(s) 262
BspT107I GGYRCC 1 cut(s) 947
BsrDI GCAATG 3 cut(s) 75, 464, 978
BsrI ACTGG 1 cut(s) 1016
BssECI CCNNGG 6 cut(s) 148, 335, 522, 847, 1159, 1160
BssMI GATC 6 cut(s) 439, 527, 990, 1054, 1091, 1242
BssNAI GTATAC 1 cut(s) 1582
BssT1I CCWWGG 2 cut(s) 335, 522
Bst1107I GTATAC 1 cut(s) 1582
Bst2UI CCWGG 2 cut(s) 149, 486
Bst4CI ACNGT 4 cut(s) 194, 349, 763, 1360
BstAPI GCANNNNNTGC 1 cut(s) 623
BstBI TTCGAA 1 cut(s) 262
BstC8I GCNNGC 5 cut(s) 96, 358, 798, 844, 1076
BstDEI CTNAG 7 cut(s) 211, 510, 670, 952, 971, 1126, 1341
BstEII GGTNACC 1 cut(s) 1014
BstF5I GGATG 2 cut(s) 100, 888
BstFNI CGCG 1 cut(s) 248
BstHHI GCGC 3 cut(s) 248, 796, 1294
BstKTI GATC 6 cut(s) 442, 530, 993, 1057, 1094, 1245
BstMBI GATC 6 cut(s) 439, 527, 990, 1054, 1091, 1242
BstMWI GCNNNNNNNGC 4 cut(s) 245, 254, 623, 843
BstNI CCWGG 2 cut(s) 149, 486
BstNSI RCATGY 2 cut(s) 98, 1573
BstPI GGTNACC 1 cut(s) 1014
BstSCI CCNGG 6 cut(s) 147, 484, 847, 1005, 1158, 1159
BstSFI CTRYAG 4 cut(s) 310, 661, 753, 1114
BstUI CGCG 1 cut(s) 248
BstV1I GCAGC 6 cut(s) 50, 141, 613, 995, 1009, 1306
BstX2I RGATCY 1 cut(s) 527
BstXI CCANNNNNNTGG 1 cut(s) 230
BstYI RGATCY 1 cut(s) 527
BstZ17I GTATAC 1 cut(s) 1582
BsuRI GGCC 6 cut(s) 26, 303, 332, 904, 1131, 1374
BtsCI GGATG 2 cut(s) 100, 888
BtsIMutI CAGTG 3 cut(s) 651, 777, 960
BveI ACCTGC 2 cut(s) 707, 1027
Cac8I GCNNGC 5 cut(s) 96, 358, 798, 844, 1076
CfoI GCGC 3 cut(s) 248, 796, 1294
Cfr13I GGNCC 5 cut(s) 302, 331, 830, 1329, 1373
Cfr9I CCCGGG 1 cut(s) 1159
Csp6I GTAC 2 cut(s) 686, 948
CspCI CAANNNNNGTGG 2 cut(s) 16, 51
CviAII CATG 9 cut(s) 95, 319, 631, 683, 900, 960, 1133, 1438, 1570
CviQI GTAC 2 cut(s) 686, 948
DdeI CTNAG 7 cut(s) 211, 510, 670, 952, 971, 1126, 1341
DpnI GATC 6 cut(s) 441, 529, 992, 1056, 1093, 1244
DpnII GATC 6 cut(s) 439, 527, 990, 1054, 1091, 1242
DraI TTTAAA 1 cut(s) 858
EaeI YGGCCR 1 cut(s) 24
EciI GGCGGA 1 cut(s) 1024
Eco130I CCWWGG 2 cut(s) 335, 522
Eco24I GRGCYC 1 cut(s) 1167
Eco47I GGWCC 2 cut(s) 830, 1329
Eco57I CTGAAG 2 cut(s) 1290, 1308
Eco88I CYCGRG 1 cut(s) 1159
Eco91I GGTNACC 1 cut(s) 1014
EcoO109I RGGNCCY 2 cut(s) 331, 1329
EcoO65I GGTNACC 1 cut(s) 1014
EcoRII CCWGG 2 cut(s) 147, 484
EcoT14I CCWWGG 2 cut(s) 335, 522
EcoT38I GRGCYC 1 cut(s) 1167
ErhI CCWWGG 2 cut(s) 335, 522
FaeI CATG 9 cut(s) 98, 322, 634, 686, 903, 963, 1136, 1441, 1573
FatI CATG 9 cut(s) 94, 318, 630, 682, 899, 959, 1132, 1437, 1569
FbaI TGATCA 2 cut(s) 990, 1242
FblI GTMKAC 1 cut(s) 1581
Fnu4HI GCNGC 6 cut(s) 39, 155, 627, 984, 1023, 1295
FokI GGATG 2 cut(s) 87, 875
FriOI GRGCYC 1 cut(s) 1167
Fsp4HI GCNGC 6 cut(s) 39, 155, 627, 984, 1023, 1295
FspBI CTAG 3 cut(s) 638, 843, 1517
GlaI GCGC 3 cut(s) 247, 795, 1293
GluI GCNGC 6 cut(s) 39, 155, 627, 984, 1023, 1295
GsaI CCCAGC 3 cut(s) 1106, 1379, 1524
HaeIII GGCC 6 cut(s) 26, 303, 332, 904, 1131, 1374
HapII CCGG 3 cut(s) 849, 1007, 1160
HhaI GCGC 3 cut(s) 248, 796, 1294
Hin1II CATG 9 cut(s) 98, 322, 634, 686, 903, 963, 1136, 1441, 1573
Hin6I GCGC 3 cut(s) 246, 794, 1292
HinP1I GCGC 3 cut(s) 246, 794, 1292
HincII GTYRAC 3 cut(s) 886, 1138, 1477
HindII GTYRAC 3 cut(s) 886, 1138, 1477
HinfI GANTC 6 cut(s) 340, 401, 428, 634, 1278, 1447
HpaI GTTAAC 1 cut(s) 886
HpaII CCGG 3 cut(s) 849, 1007, 1160
HphI GGTGA 6 cut(s) 206, 337, 437, 453, 1008, 1237
Hpy166II GTNNAC 9 cut(s) 7, 643, 886, 1001, 1138, 1261, 1435, 1477, 1582
Hpy188I TCNGA 6 cut(s) 170, 788, 1215, 1270, 1344, 1567
Hpy188III TCNNGA 4 cut(s) 104, 227, 825, 994
Hpy8I GTNNAC 9 cut(s) 7, 643, 886, 1001, 1138, 1261, 1435, 1477, 1582
HpyAV CCTTC 5 cut(s) 191, 539, 1064, 1317, 1461
HpyCH4III ACNGT 4 cut(s) 194, 349, 763, 1360
HpyCH4V TGCA 7 cut(s) 38, 94, 124, 157, 491, 899, 983
HpyF10VI GCNNNNNNNGC 4 cut(s) 245, 254, 623, 843
HpyF3I CTNAG 7 cut(s) 211, 510, 670, 952, 971, 1126, 1341
Hsp92II CATG 9 cut(s) 98, 322, 634, 686, 903, 963, 1136, 1441, 1573
HspAI GCGC 3 cut(s) 246, 794, 1292
KpnI GGTACC 1 cut(s) 951
Ksp22I TGATCA 2 cut(s) 990, 1242
KspAI GTTAAC 1 cut(s) 886
Kzo9I GATC 6 cut(s) 439, 527, 990, 1054, 1091, 1242
LmnI GCTCC 3 cut(s) 254, 353, 805
Lsp1109I GCAGC 6 cut(s) 50, 141, 613, 995, 1009, 1306
LweI GCATC 3 cut(s) 667, 735, 781
MaeI CTAG 3 cut(s) 638, 843, 1517
MaeIII GTNAC 5 cut(s) 922, 1014, 1279, 1360, 1444
MalI GATC 6 cut(s) 441, 529, 992, 1056, 1093, 1244
MboI GATC 6 cut(s) 439, 527, 990, 1054, 1091, 1242
MboII GAAGA 4 cut(s) 597, 614, 1199, 1411
MfeI CAATTG 2 cut(s) 804, 1080
MflI RGATCY 1 cut(s) 527
MhlI GDGCHC 1 cut(s) 1167
MlsI TGGCCA 1 cut(s) 26
MluNI TGGCCA 1 cut(s) 26
MlyI GAGTC 2 cut(s) 1287, 1441
MmeI TCCRAC 1 cut(s) 331
Mox20I TGGCCA 1 cut(s) 26
MscI TGGCCA 1 cut(s) 26
MseI TTAA 3 cut(s) 857, 885, 1612
MslI CAYNNNNRTG 3 cut(s) 228, 972, 1442
Msp20I TGGCCA 1 cut(s) 26
MspA1I CMGCKG 3 cut(s) 282, 1052, 1286
MspI CCGG 3 cut(s) 849, 1007, 1160
MspR9I CCNGG 6 cut(s) 149, 486, 849, 1007, 1160, 1161
MunI CAATTG 2 cut(s) 804, 1080
MvaI CCWGG 2 cut(s) 149, 486
MvnI CGCG 1 cut(s) 248
MwoI GCNNNNNNNGC 4 cut(s) 245, 254, 623, 843
NciI CCSGG 4 cut(s) 849, 1007, 1160, 1161
NdeII GATC 6 cut(s) 439, 527, 990, 1054, 1091, 1242
NheI GCTAGC 1 cut(s) 842
NlaIII CATG 9 cut(s) 98, 322, 634, 686, 903, 963, 1136, 1441, 1573
NlaIV GGNNCC 3 cut(s) 333, 949, 1330
NmuCI GTSAC 5 cut(s) 922, 1014, 1279, 1360, 1444
NspI RCATGY 2 cut(s) 98, 1573
NspV TTCGAA 1 cut(s) 262
OliI CACNNNNGTG 1 cut(s) 972
PaeI GCATGC 1 cut(s) 98
PaqCI CACCTGC 1 cut(s) 1027
PciI ACATGT 1 cut(s) 1569
PfeI GAWTC 4 cut(s) 340, 401, 428, 634
PkrI GCNGC 6 cut(s) 40, 156, 628, 985, 1024, 1296
PleI GAGTC 2 cut(s) 1286, 1441
PpsI GAGTC 2 cut(s) 1286, 1441
PpuMI RGGWCCY 1 cut(s) 1329
PscI ACATGT 1 cut(s) 1569
Psp5II RGGWCCY 1 cut(s) 1329
Psp6I CCWGG 2 cut(s) 147, 484
PspEI GGTNACC 1 cut(s) 1014
PspFI CCCAGC 3 cut(s) 1102, 1375, 1520
PspGI CCWGG 2 cut(s) 147, 484
PspN4I GGNNCC 3 cut(s) 333, 949, 1330
PspPI GGNCC 5 cut(s) 302, 331, 830, 1329, 1373
PspPPI RGGWCCY 1 cut(s) 1329
PsuI RGATCY 1 cut(s) 527
PvuII CAGCTG 2 cut(s) 282, 1286
RsaI GTAC 2 cut(s) 687, 949
RsaNI GTAC 2 cut(s) 686, 948
RseI CAYNNNNRTG 3 cut(s) 228, 972, 1442
SaqAI TTAA 3 cut(s) 857, 885, 1612
SatI GCNGC 6 cut(s) 39, 155, 627, 984, 1023, 1295
Sau3AI GATC 6 cut(s) 439, 527, 990, 1054, 1091, 1242
Sau96I GGNCC 5 cut(s) 302, 331, 830, 1329, 1373
SchI GAGTC 2 cut(s) 1287, 1441
ScrFI CCNGG 6 cut(s) 149, 486, 849, 1007, 1160, 1161
SduI GDGCHC 1 cut(s) 1167
SfaNI GCATC 3 cut(s) 667, 735, 781
SfcI CTRYAG 4 cut(s) 310, 661, 753, 1114
SfuI TTCGAA 1 cut(s) 262
SinI GGWCC 2 cut(s) 830, 1329
SmaI CCCGGG 1 cut(s) 1161
SmiMI CAYNNNNRTG 3 cut(s) 228, 972, 1442
SphI GCATGC 1 cut(s) 98
SsiI CCGC 2 cut(s) 1035, 1050
SspI AATATT 1 cut(s) 413
SspMI CTAG 3 cut(s) 638, 843, 1517
StyD4I CCNGG 6 cut(s) 147, 484, 847, 1005, 1158, 1159
StyI CCWWGG 2 cut(s) 335, 522
TaaI ACNGT 4 cut(s) 194, 349, 763, 1360
TaqI TCGA 4 cut(s) 262, 438, 1068, 1397
TaqII GACCGA 1 cut(s) 847
TatI WGTACW 1 cut(s) 685
TfiI GAWTC 4 cut(s) 340, 401, 428, 634
Tru1I TTAA 3 cut(s) 857, 885, 1612
Tru9I TTAA 3 cut(s) 857, 885, 1612
TscAI CASTG 3 cut(s) 658, 784, 960
TseFI GTSAC 5 cut(s) 922, 1014, 1279, 1360, 1444
TseI GCWGC 6 cut(s) 38, 154, 626, 983, 1022, 1294
Tsp45I GTSAC 5 cut(s) 922, 1014, 1279, 1360, 1444
TspDTI ATGAA 4 cut(s) 810, 1299, 1456, 1524
TspMI CCCGGG 1 cut(s) 1159
TspRI CASTG 3 cut(s) 658, 784, 960
VpaK11BI GGWCC 2 cut(s) 830, 1329
XapI RAATTY 3 cut(s) 270, 448, 852
XceI RCATGY 2 cut(s) 98, 1573
XmaI CCCGGG 1 cut(s) 1159
XmiI GTMKAC 1 cut(s) 1581
XspI CTAG 3 cut(s) 638, 843, 1517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.