MD04G1072700.v1.1

PA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000302
Physical Location & Seq
Reverse (-)
9922017 .. 9923048
1032 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1072700.v1.1.491

Sequence Viewer

Length: 1032 bp
ATGATGAAAAAAAACAGCATGCAAACTTATATAGTTTGGGTTGAAAAGCCGGTCTCTCAGAAATTTTCTCCGCAATCGAATGAAGATTTAGGGAGTTGGTACCAGTCATTCTTGCCTGAAAGTATTGCAAGCTCAAACCAACAGATGAACCAGCGAATGGTTCATTCATACCGCAATATAGCGACAGGTTTTGCAGCAAAACTGACACCCGAGGAAGTGAGAGAAATGGAAACAAAAGAAGGGTTCGTCTCAGCTCGTCCACAAAGAATTCTGCCGTTGCAGACTACTCATACTCCTGACTTCTTGGGGTTGCACCAAGGGTCCGGACTTTGGGAAGCAGCAACTTACGGTAAAGGTGTAATAATTGGACTTTTAGATACCGGGATTGTACCAGATCATCCCTCGTTTAATGACGAAGGAATGCCCCCTCCTCCAGCAAAATGGAAGGGCAAGTGCGAGTTCAATGAGACATTGTGCAATAACAAGCTTATTGGTGCAAGAAATTTCATAGGCGTAGGCAAAGGCCAACCAAAACGAAGATTTCCATTTGACATAGCTGGTCATGGAAGCCACACATCTAGCACAGCTGCCGGAAGGTTTGTGGAAGGCGCAAGCGTGTATGGGCAGGCCAATGGCACAGCTGCTGGCATGGCACCATATGCTCACTTGGCAATGTACAAAGTCTGCGAAGGCTTTGGGTGTGCCGATGCTGACATTTTTGCTGCTCTGGACGTCGCTATTGATGATGGAGTTGACGTACTCTCCCTCTCACTTGGTGGCCCCTCACTTCCTTTCTATGATGATGTAATTGCAATTGGTGCATTTGCAGCAATTCAAAAGGGAATTTTTTTCAGCTGTGCAGGTGGAAATTCGGGTCCTTGGAATCGCACCTTATCAAATGATGCACCATGGATACTCACAGTTGGAGCAAGCACCACCGACAGAATATTAACATCTGGATTGCAGATTGGAGACGAAAAACAGTACCATTTTGATGGAAAATCTTTGTCCCAGCCTGAAGATTTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

344

Amino Acids

37.07

Weight (kDa)

5.51

Isoelectric Point (pI)

37.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 9 - 93 1.6e-09 Peptidase inhibitor I9
Peptidase_S8 PF00082 117 - 313 1.2e-21 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 851
AatII GACGTC 1 cut(s) 735
Acc36I ACCTGC 1 cut(s) 851
Acc65I GGTACC 1 cut(s) 99
AccB1I GGYRCC 2 cut(s) 99, 652
AccB7I CCANNNNNTGG 1 cut(s) 157
AccIII TCCGGA 1 cut(s) 323
AciI CCGC 2 cut(s) 71, 172
AcsI RAATTY 5 cut(s) 62, 267, 502, 843, 868
AcyI GRCGYC 1 cut(s) 732
AdeI CACNNNGTG 1 cut(s) 776
AfaI GTAC 5 cut(s) 101, 390, 677, 759, 986
AfiI CCNNNNNNNGG 2 cut(s) 157, 330
AgsI TTSAA 3 cut(s) 44, 463, 836
AluBI AGCT 7 cut(s) 132, 254, 487, 557, 587, 641, 855
AluI AGCT 7 cut(s) 132, 254, 487, 557, 587, 641, 855
Alw26I GTCTC 4 cut(s) 58, 253, 461, 966
AlwNI CAGNNNCTG 1 cut(s) 644
Ama87I CYCGRG 1 cut(s) 209
Aor13HI TCCGGA 1 cut(s) 323
AoxI GGCC 3 cut(s) 523, 627, 778
ApeKI GCWGC 6 cut(s) 194, 338, 587, 641, 722, 827
ApoI RAATTY 5 cut(s) 62, 267, 502, 843, 868
Asp718I GGTACC 1 cut(s) 99
AspLEI GCGC 1 cut(s) 611
AspS9I GGNCC 3 cut(s) 321, 779, 875
AsuC2I CCSGG 1 cut(s) 382
AvaI CYCGRG 1 cut(s) 209
AvaII GGWCC 2 cut(s) 321, 875
BanI GGYRCC 2 cut(s) 99, 652
BbvI GCAGC 6 cut(s) 206, 350, 574, 628, 709, 839
BccI CCATC 2 cut(s) 740, 989
BceAI ACGGC 1 cut(s) 259
BciVI GTATCC 1 cut(s) 906
BcnI CCSGG 1 cut(s) 382
BcoDI GTCTC 4 cut(s) 58, 253, 461, 966
BfaI CTAG 1 cut(s) 579
BfuAI ACCTGC 1 cut(s) 851
BfuI GTATCC 1 cut(s) 906
BisI GCNGC 6 cut(s) 195, 339, 588, 642, 723, 828
BlsI GCNGC 6 cut(s) 196, 340, 589, 643, 724, 829
Bme1390I CCNGG 1 cut(s) 382
Bme18I GGWCC 2 cut(s) 321, 875
BmeT110I CYCGRG 1 cut(s) 209
BmgT120I GGNCC 3 cut(s) 321, 779, 875
BmiI GGNNCC 5 cut(s) 101, 322, 654, 781, 876
BmrFI CCNGG 1 cut(s) 382
BmsI GCATC 2 cut(s) 697, 892
BpmI CTGGAG 1 cut(s) 417
BpuMI CCSGG 1 cut(s) 382
BsaHI GRCGYC 1 cut(s) 732
BsaI GGTCTC 1 cut(s) 58
BsaJI CCNNGG 4 cut(s) 210, 316, 878, 908
BsaWI WCCGGW 1 cut(s) 323
BsaXI ACNNNNNCTCC 6 cut(s) 277, 307, 741, 746, 771, 776
Bsc4I CCNNNNNNNGG 2 cut(s) 157, 330
Bse118I RCCGGY 1 cut(s) 49
Bse1I ACTGG 1 cut(s) 103
Bse3DI GCAATG 1 cut(s) 678
BseAI TCCGGA 1 cut(s) 323
BseDI CCNNGG 4 cut(s) 210, 316, 878, 908
BseGI GGATG 1 cut(s) 397
BseLI CCNNNNNNNGG 2 cut(s) 157, 330
BseMI GCAATG 1 cut(s) 678
BseMII CTCAG 2 cut(s) 71, 264
BseNI ACTGG 1 cut(s) 103
BseRI GAGGAG 1 cut(s) 420
BseXI GCAGC 6 cut(s) 206, 350, 574, 628, 709, 839
BseYI CCCAGC 1 cut(s) 1011
BsgI GTGCAG 1 cut(s) 879
BshFI GGCC 3 cut(s) 525, 629, 780
BshNI GGYRCC 2 cut(s) 99, 652
BsiHKCI CYCGRG 1 cut(s) 209
BsiSI CCGG 4 cut(s) 50, 324, 381, 591
BslFI GGGAC 1 cut(s) 994
BslI CCNNNNNNNGG 2 cut(s) 157, 330
BsmAI GTCTC 4 cut(s) 58, 253, 461, 966
BsmBI CGTCTC 2 cut(s) 253, 966
BsmFI GGGAC 1 cut(s) 994
BsmI GAATGC 1 cut(s) 426
BsnI GGCC 3 cut(s) 525, 629, 780
Bso31I GGTCTC 1 cut(s) 58
BsoBI CYCGRG 1 cut(s) 209
Bsp13I TCCGGA 1 cut(s) 323
Bsp1407I TGTACA 1 cut(s) 675
Bsp143I GATC 1 cut(s) 394
Bsp19I CCATGG 1 cut(s) 908
BspACI CCGC 2 cut(s) 71, 172
BspANI GGCC 3 cut(s) 525, 629, 780
BspCNI CTCAG 2 cut(s) 70, 263
BspEI TCCGGA 1 cut(s) 323
BspLI GGNNCC 5 cut(s) 101, 322, 654, 781, 876
BspMI ACCTGC 1 cut(s) 851
BspT107I GGYRCC 2 cut(s) 99, 652
BspTNI GGTCTC 1 cut(s) 58
BsrDI GCAATG 1 cut(s) 678
BsrFI RCCGGY 1 cut(s) 49
BsrGI TGTACA 1 cut(s) 675
BsrI ACTGG 1 cut(s) 103
BssAI RCCGGY 1 cut(s) 49
BssECI CCNNGG 4 cut(s) 210, 316, 878, 908
BssMI GATC 1 cut(s) 394
BssNI GRCGYC 1 cut(s) 732
BssT1I CCWWGG 3 cut(s) 316, 878, 908
Bst4CI ACNGT 3 cut(s) 350, 922, 984
BstACI GRCGYC 1 cut(s) 732
BstAPI GCANNNNNTGC 2 cut(s) 659, 818
BstAUI TGTACA 1 cut(s) 675
BstC8I GCNNGC 6 cut(s) 20, 130, 613, 627, 646, 931
BstDEI CTNAG 2 cut(s) 57, 250
BstDSI CCRYGG 1 cut(s) 908
BstF5I GGATG 1 cut(s) 397
BstHHI GCGC 1 cut(s) 611
BstKTI GATC 1 cut(s) 397
BstMAI GTCTC 4 cut(s) 58, 253, 461, 966
BstMBI GATC 1 cut(s) 394
BstMWI GCNNNNNNNGC 5 cut(s) 650, 659, 668, 818, 827
BstNSI RCATGY 1 cut(s) 22
BstSCI CCNGG 1 cut(s) 380
BstV1I GCAGC 6 cut(s) 206, 350, 574, 628, 709, 839
BstXI CCANNNNNNTGG 2 cut(s) 441, 995
BsuI GTATCC 1 cut(s) 906
BsuRI GGCC 3 cut(s) 525, 629, 780
BtgI CCRYGG 1 cut(s) 908
BtsCI GGATG 1 cut(s) 397
BveI ACCTGC 1 cut(s) 851
Cac8I GCNNGC 6 cut(s) 20, 130, 613, 627, 646, 931
CaiI CAGNNNCTG 1 cut(s) 644
CfoI GCGC 1 cut(s) 611
Cfr10I RCCGGY 1 cut(s) 49
Cfr13I GGNCC 3 cut(s) 321, 779, 875
Csp6I GTAC 5 cut(s) 100, 389, 676, 758, 985
CviAII CATG 4 cut(s) 19, 563, 649, 909
CviQI GTAC 5 cut(s) 100, 389, 676, 758, 985
DdeI CTNAG 2 cut(s) 57, 250
DpnI GATC 1 cut(s) 396
DpnII GATC 1 cut(s) 394
DraIII CACNNNGTG 1 cut(s) 776
Eco130I CCWWGG 3 cut(s) 316, 878, 908
Eco31I GGTCTC 1 cut(s) 58
Eco47I GGWCC 2 cut(s) 321, 875
Eco88I CYCGRG 1 cut(s) 209
EcoO109I RGGNCCY 1 cut(s) 875
EcoRI GAATTC 1 cut(s) 267
EcoT14I CCWWGG 3 cut(s) 316, 878, 908
ErhI CCWWGG 3 cut(s) 316, 878, 908
Esp3I CGTCTC 2 cut(s) 253, 966
FaeI CATG 4 cut(s) 22, 566, 652, 912
FaqI GGGAC 1 cut(s) 994
FatI CATG 4 cut(s) 18, 562, 648, 908
FauNDI CATATG 1 cut(s) 658
Fnu4HI GCNGC 6 cut(s) 195, 339, 588, 642, 723, 828
FokI GGATG 1 cut(s) 384
Fsp4HI GCNGC 6 cut(s) 195, 339, 588, 642, 723, 828
FspBI CTAG 1 cut(s) 579
GlaI GCGC 1 cut(s) 610
GluI GCNGC 6 cut(s) 195, 339, 588, 642, 723, 828
GsaI CCCAGC 1 cut(s) 1015
GsuI CTGGAG 1 cut(s) 417
HaeIII GGCC 3 cut(s) 525, 629, 780
HapII CCGG 4 cut(s) 50, 324, 381, 591
HhaI GCGC 1 cut(s) 611
Hin1I GRCGYC 1 cut(s) 732
Hin1II CATG 4 cut(s) 22, 566, 652, 912
Hin6I GCGC 1 cut(s) 609
HinP1I GCGC 1 cut(s) 609
HincII GTYRAC 1 cut(s) 754
HindII GTYRAC 1 cut(s) 754
HindIII AAGCTT 1 cut(s) 485
HinfI GANTC 1 cut(s) 883
HpaII CCGG 4 cut(s) 50, 324, 381, 591
Hpy166II GTNNAC 2 cut(s) 260, 754
Hpy188I TCNGA 1 cut(s) 60
Hpy188III TCNNGA 4 cut(s) 296, 324, 728, 957
Hpy8I GTNNAC 2 cut(s) 260, 754
Hpy99I CGWCG 1 cut(s) 737
HpyAV CCTTC 6 cut(s) 233, 410, 439, 588, 599, 683
HpyCH4III ACNGT 3 cut(s) 350, 922, 984
HpyCH4IV ACGT 2 cut(s) 732, 756
HpyF10VI GCNNNNNNNGC 5 cut(s) 650, 659, 668, 818, 827
HpyF3I CTNAG 2 cut(s) 57, 250
HpySE526I ACGT 2 cut(s) 732, 756
Hsp92I GRCGYC 1 cut(s) 732
Hsp92II CATG 4 cut(s) 22, 566, 652, 912
HspAI GCGC 1 cut(s) 609
Kpn2I TCCGGA 1 cut(s) 323
KpnI GGTACC 1 cut(s) 103
Kzo9I GATC 1 cut(s) 394
LmnI GCTCC 1 cut(s) 926
Lsp1109I GCAGC 6 cut(s) 206, 350, 574, 628, 709, 839
LweI GCATC 2 cut(s) 697, 892
MaeI CTAG 1 cut(s) 579
MaeII ACGT 2 cut(s) 732, 756
MalI GATC 1 cut(s) 396
MboI GATC 1 cut(s) 394
MboII GAAGA 3 cut(s) 95, 549, 1031
MfeI CAATTG 1 cut(s) 813
MluCI AATT 9 cut(s) 62, 267, 363, 502, 807, 813, 831, 843, 868
MmeI TCCRAC 1 cut(s) 904
MnlI CCTC 6 cut(s) 205, 412, 438, 441, 776, 793
MroI TCCGGA 1 cut(s) 323
MseI TTAA 3 cut(s) 408, 950, 1030
MslI CAYNNNNRTG 1 cut(s) 993
MspA1I CMGCKG 3 cut(s) 587, 641, 855
MspI CCGG 4 cut(s) 50, 324, 381, 591
MspR9I CCNGG 1 cut(s) 382
MunI CAATTG 1 cut(s) 813
Mva1269I GAATGC 1 cut(s) 426
MwoI GCNNNNNNNGC 5 cut(s) 650, 659, 668, 818, 827
NciI CCSGG 1 cut(s) 382
NcoI CCATGG 1 cut(s) 908
NdeI CATATG 1 cut(s) 658
NdeII GATC 1 cut(s) 394
NlaIII CATG 4 cut(s) 22, 566, 652, 912
NlaIV GGNNCC 5 cut(s) 101, 322, 654, 781, 876
NspI RCATGY 1 cut(s) 22
PaeI GCATGC 1 cut(s) 22
PaqCI CACCTGC 1 cut(s) 851
PctI GAATGC 1 cut(s) 426
PfeI GAWTC 1 cut(s) 883
PflMI CCANNNNNTGG 1 cut(s) 157
PkrI GCNGC 6 cut(s) 196, 340, 589, 643, 724, 829
PpuMI RGGWCCY 1 cut(s) 875
Psp5II RGGWCCY 1 cut(s) 875
PspFI CCCAGC 1 cut(s) 1011
PspN4I GGNNCC 5 cut(s) 101, 322, 654, 781, 876
PspPI GGNCC 3 cut(s) 321, 779, 875
PspPPI RGGWCCY 1 cut(s) 875
PstNI CAGNNNCTG 1 cut(s) 644
PvuII CAGCTG 3 cut(s) 587, 641, 855
RsaI GTAC 5 cut(s) 101, 390, 677, 759, 986
RsaNI GTAC 5 cut(s) 100, 389, 676, 758, 985
RseI CAYNNNNRTG 1 cut(s) 993
SaqAI TTAA 3 cut(s) 408, 950, 1030
SatI GCNGC 6 cut(s) 195, 339, 588, 642, 723, 828
Sau3AI GATC 1 cut(s) 394
Sau96I GGNCC 3 cut(s) 321, 779, 875
ScrFI CCNGG 1 cut(s) 382
SfaNI GCATC 2 cut(s) 697, 892
SinI GGWCC 2 cut(s) 321, 875
SmiMI CAYNNNNRTG 1 cut(s) 993
SphI GCATGC 1 cut(s) 22
Sse9I AATT 9 cut(s) 62, 267, 363, 502, 807, 813, 831, 843, 868
SsiI CCGC 2 cut(s) 71, 172
SspI AATATT 1 cut(s) 948
SspMI CTAG 1 cut(s) 579
StyD4I CCNGG 1 cut(s) 380
StyI CCWWGG 3 cut(s) 316, 878, 908
TaaI ACNGT 3 cut(s) 350, 922, 984
TaiI ACGT 2 cut(s) 735, 759
TaqI TCGA 1 cut(s) 77
TasI AATT 9 cut(s) 62, 267, 363, 502, 807, 813, 831, 843, 868
TatI WGTACW 1 cut(s) 675
TfiI GAWTC 1 cut(s) 883
Tru1I TTAA 3 cut(s) 408, 950, 1030
Tru9I TTAA 3 cut(s) 408, 950, 1030
TseI GCWGC 6 cut(s) 194, 338, 587, 641, 722, 827
TspDTI ATGAA 6 cut(s) 20, 96, 152, 156, 161, 496
Van91I CCANNNNNTGG 1 cut(s) 157
VpaK11BI GGWCC 2 cut(s) 321, 875
XapI RAATTY 5 cut(s) 62, 267, 502, 843, 868
XceI RCATGY 1 cut(s) 22
XspI CTAG 1 cut(s) 579
ZraI GACGTC 1 cut(s) 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.