subtilisin-like protease
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1142 |
| Acc36I | ACCTGC | 1 cut(s) | 1142 |
| Acc65I | GGTACC | 1 cut(s) | 210 |
| AccB1I | GGYRCC | 4 cut(s) | 210, 605, 710, 754 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 289 |
| AccBSI | CCGCTC | 1 cut(s) | 365 |
| AciI | CCGC | 3 cut(s) | 110, 320, 363 |
| AclWI | GGATC | 2 cut(s) | 676, 1201 |
| AcoI | YGGCCR | 1 cut(s) | 729 |
| AcsI | RAATTY | 4 cut(s) | 169, 379, 480, 697 |
| AcyI | GRCGYC | 1 cut(s) | 852 |
| AdeI | CACNNNGTG | 2 cut(s) | 289, 878 |
| AfaI | GTAC | 2 cut(s) | 212, 534 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 289, 458, 1025 |
| AflIII | ACRYGT | 1 cut(s) | 675 |
| AgsI | TTSAA | 2 cut(s) | 332, 1128 |
| AjiI | CACGTC | 1 cut(s) | 678 |
| Alw21I | GWGCWC | 2 cut(s) | 1037, 1159 |
| Alw44I | GTGCAC | 1 cut(s) | 1155 |
| AlwI | GGATC | 2 cut(s) | 676, 1201 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 83, 746 |
| Ama87I | CYCGRG | 1 cut(s) | 254 |
| AoxI | GGCC | 5 cut(s) | 449, 640, 661, 729, 976 |
| ApaLI | GTGCAC | 1 cut(s) | 1155 |
| ApeKI | GCWGC | 6 cut(s) | 95, 98, 305, 421, 424, 824 |
| ApoI | RAATTY | 4 cut(s) | 169, 379, 480, 697 |
| Asp718I | GGTACC | 1 cut(s) | 210 |
| AspLEI | GCGC | 1 cut(s) | 923 |
| AspS9I | GGNCC | 3 cut(s) | 449, 641, 977 |
| AsuHPI | GGTGA | 1 cut(s) | 473 |
| AsuNHI | GCTAGC | 1 cut(s) | 112 |
| AvaI | CYCGRG | 1 cut(s) | 254 |
| BaeGI | GKGCMC | 1 cut(s) | 1159 |
| BalI | TGGCCA | 1 cut(s) | 731 |
| BanI | GGYRCC | 4 cut(s) | 210, 605, 710, 754 |
| Bbv12I | GWGCWC | 2 cut(s) | 1037, 1159 |
| BbvI | GCAGC | 6 cut(s) | 107, 110, 292, 408, 436, 811 |
| BccI | CCATC | 6 cut(s) | 331, 344, 516, 745, 842, 899 |
| BceAI | ACGGC | 1 cut(s) | 751 |
| BfaI | CTAG | 2 cut(s) | 74, 113 |
| BfmI | CTRYAG | 1 cut(s) | 422 |
| BfuAI | ACCTGC | 1 cut(s) | 1142 |
| BisI | GCNGC | 7 cut(s) | 96, 99, 110, 306, 422, 425, 825 |
| BlpI | GCTNAGC | 2 cut(s) | 301, 886 |
| BlsI | GCNGC | 7 cut(s) | 97, 100, 111, 307, 423, 426, 826 |
| BmeT110I | CYCGRG | 1 cut(s) | 254 |
| BmgBI | CACGTC | 1 cut(s) | 678 |
| BmgT120I | GGNCC | 3 cut(s) | 449, 641, 977 |
| BmiI | GGNNCC | 5 cut(s) | 108, 212, 607, 712, 756 |
| BmrI | ACTGGG | 1 cut(s) | 756 |
| BmsI | GCATC | 1 cut(s) | 15 |
| BmtI | GCTAGC | 1 cut(s) | 116 |
| BmuI | ACTGGG | 1 cut(s) | 756 |
| BpmI | CTGGAG | 4 cut(s) | 528, 630, 657, 711 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 301, 886 |
| BsaBI | GATNNNNATC | 1 cut(s) | 29 |
| BsaHI | GRCGYC | 1 cut(s) | 852 |
| BsaJI | CCNNGG | 3 cut(s) | 664, 1010, 1020 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 289, 458, 1025 |
| Bse118I | RCCGGY | 1 cut(s) | 160 |
| Bse1I | ACTGG | 5 cut(s) | 301, 640, 647, 714, 751 |
| Bse8I | GATNNNNATC | 1 cut(s) | 29 |
| BseDI | CCNNGG | 3 cut(s) | 664, 1010, 1020 |
| BseGI | GGATG | 3 cut(s) | 367, 508, 756 |
| BseJI | GATNNNNATC | 1 cut(s) | 29 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 289, 458, 1025 |
| BseMII | CTCAG | 2 cut(s) | 804, 900 |
| BseNI | ACTGG | 5 cut(s) | 301, 640, 647, 714, 751 |
| BseSI | GKGCMC | 1 cut(s) | 1159 |
| BseXI | GCAGC | 6 cut(s) | 107, 110, 292, 408, 436, 811 |
| BshFI | GGCC | 5 cut(s) | 451, 642, 663, 731, 978 |
| BshNI | GGYRCC | 4 cut(s) | 210, 605, 710, 754 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1037, 1159 |
| BsiHKCI | CYCGRG | 1 cut(s) | 254 |
| BsiSI | CCGG | 1 cut(s) | 161 |
| BslFI | GGGAC | 2 cut(s) | 450, 592 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 289, 458, 1025 |
| BsmFI | GGGAC | 2 cut(s) | 450, 592 |
| BsnI | GGCC | 5 cut(s) | 451, 642, 663, 731, 978 |
| BsoBI | CYCGRG | 1 cut(s) | 254 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1037, 1159 |
| Bsp143I | GATC | 3 cut(s) | 30, 668, 1193 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 301, 886 |
| Bsp19I | CCATGG | 2 cut(s) | 664, 1010 |
| BspACI | CCGC | 3 cut(s) | 110, 320, 363 |
| BspANI | GGCC | 5 cut(s) | 451, 642, 663, 731, 978 |
| BspCNI | CTCAG | 2 cut(s) | 803, 899 |
| BspLI | GGNNCC | 5 cut(s) | 108, 212, 607, 712, 756 |
| BspMAI | CTGCAG | 1 cut(s) | 426 |
| BspMI | ACCTGC | 1 cut(s) | 1142 |
| BspOI | GCTAGC | 1 cut(s) | 116 |
| BspPI | GGATC | 2 cut(s) | 676, 1201 |
| BspT107I | GGYRCC | 4 cut(s) | 210, 605, 710, 754 |
| BsrBI | CCGCTC | 1 cut(s) | 365 |
| BsrFI | RCCGGY | 1 cut(s) | 160 |
| BsrI | ACTGG | 5 cut(s) | 301, 640, 647, 714, 751 |
| BssAI | RCCGGY | 1 cut(s) | 160 |
| BssECI | CCNNGG | 3 cut(s) | 664, 1010, 1020 |
| BssMI | GATC | 3 cut(s) | 30, 668, 1193 |
| BssNI | GRCGYC | 1 cut(s) | 852 |
| BssT1I | CCWWGG | 2 cut(s) | 664, 1010 |
| Bst4CI | ACNGT | 3 cut(s) | 583, 1024, 1134 |
| Bst6I | CTCTTC | 1 cut(s) | 327 |
| BstACI | GRCGYC | 1 cut(s) | 852 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 761 |
| BstC8I | GCNNGC | 4 cut(s) | 114, 131, 976, 1033 |
| BstDEI | CTNAG | 3 cut(s) | 301, 790, 886 |
| BstDSI | CCRYGG | 3 cut(s) | 664, 1010, 1020 |
| BstF5I | GGATG | 3 cut(s) | 367, 508, 756 |
| BstHHI | GCGC | 1 cut(s) | 923 |
| BstKTI | GATC | 3 cut(s) | 33, 671, 1196 |
| BstMBI | GATC | 3 cut(s) | 30, 668, 1193 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 259, 761, 770, 920, 929, 971 |
| BstNSI | RCATGY | 1 cut(s) | 133 |
| BstSFI | CTRYAG | 1 cut(s) | 422 |
| BstSLI | GKGCMC | 1 cut(s) | 1159 |
| BstV1I | GCAGC | 6 cut(s) | 107, 110, 292, 408, 436, 811 |
| BstX2I | RGATCY | 2 cut(s) | 668, 1193 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 552 |
| BstYI | RGATCY | 2 cut(s) | 668, 1193 |
| BsuRI | GGCC | 5 cut(s) | 451, 642, 663, 731, 978 |
| BtgI | CCRYGG | 3 cut(s) | 664, 1010, 1020 |
| BtrI | CACGTC | 1 cut(s) | 678 |
| BtsCI | GGATG | 3 cut(s) | 367, 508, 756 |
| BtsI | GCAGTG | 1 cut(s) | 834 |
| BtsIMutI | CAGTG | 3 cut(s) | 580, 721, 834 |
| BveI | ACCTGC | 1 cut(s) | 1142 |
| Cac8I | GCNNGC | 4 cut(s) | 114, 131, 976, 1033 |
| CaiI | CAGNNNCTG | 2 cut(s) | 83, 746 |
| CfoI | GCGC | 1 cut(s) | 923 |
| Cfr10I | RCCGGY | 1 cut(s) | 160 |
| Cfr13I | GGNCC | 3 cut(s) | 449, 641, 977 |
| CseI | GACGC | 1 cut(s) | 841 |
| Csp6I | GTAC | 2 cut(s) | 211, 533 |
| CviAII | CATG | 6 cut(s) | 58, 130, 191, 665, 761, 1011 |
| CviQI | GTAC | 2 cut(s) | 211, 533 |
| DdeI | CTNAG | 3 cut(s) | 301, 790, 886 |
| DpnI | GATC | 3 cut(s) | 32, 670, 1195 |
| DpnII | GATC | 3 cut(s) | 30, 668, 1193 |
| DraIII | CACNNNGTG | 2 cut(s) | 289, 878 |
| EaeI | YGGCCR | 1 cut(s) | 729 |
| Eam1104I | CTCTTC | 1 cut(s) | 327 |
| EarI | CTCTTC | 1 cut(s) | 327 |
| Eco130I | CCWWGG | 2 cut(s) | 664, 1010 |
| Eco88I | CYCGRG | 1 cut(s) | 254 |
| EcoO109I | RGGNCCY | 2 cut(s) | 449, 641 |
| EcoT14I | CCWWGG | 2 cut(s) | 664, 1010 |
| EcoT22I | ATGCAT | 1 cut(s) | 280 |
| ErhI | CCWWGG | 2 cut(s) | 664, 1010 |
| FaeI | CATG | 6 cut(s) | 61, 133, 194, 668, 764, 1014 |
| FalI | AAGNNNNNCTT | 3 cut(s) | 932, 964, 1182 |
| FaqI | GGGAC | 2 cut(s) | 450, 592 |
| FatI | CATG | 6 cut(s) | 57, 129, 190, 664, 760, 1010 |
| Fnu4HI | GCNGC | 7 cut(s) | 96, 99, 110, 306, 422, 425, 825 |
| FokI | GGATG | 3 cut(s) | 354, 495, 763 |
| Fsp4HI | GCNGC | 7 cut(s) | 96, 99, 110, 306, 422, 425, 825 |
| FspBI | CTAG | 2 cut(s) | 74, 113 |
| GlaI | GCGC | 1 cut(s) | 922 |
| GluI | GCNGC | 7 cut(s) | 96, 99, 110, 306, 422, 425, 825 |
| GsuI | CTGGAG | 4 cut(s) | 528, 630, 657, 711 |
| HaeIII | GGCC | 5 cut(s) | 451, 642, 663, 731, 978 |
| HapII | CCGG | 1 cut(s) | 161 |
| HgaI | GACGC | 1 cut(s) | 841 |
| HhaI | GCGC | 1 cut(s) | 923 |
| Hin1I | GRCGYC | 1 cut(s) | 852 |
| Hin1II | CATG | 6 cut(s) | 61, 133, 194, 668, 764, 1014 |
| Hin6I | GCGC | 1 cut(s) | 921 |
| HinP1I | GCGC | 1 cut(s) | 921 |
| HincII | GTYRAC | 1 cut(s) | 652 |
| HindII | GTYRAC | 1 cut(s) | 652 |
| HindIII | AAGCTT | 2 cut(s) | 596, 1067 |
| HinfI | GANTC | 5 cut(s) | 809, 945, 1015, 1097, 1169 |
| HpaII | CCGG | 1 cut(s) | 161 |
| HphI | GGTGA | 1 cut(s) | 473 |
| Hpy166II | GTNNAC | 2 cut(s) | 652, 1157 |
| Hpy188I | TCNGA | 6 cut(s) | 167, 187, 793, 808, 814, 939 |
| Hpy188III | TCNNGA | 3 cut(s) | 256, 371, 545 |
| Hpy8I | GTNNAC | 2 cut(s) | 652, 1157 |
| Hpy99I | CGWCG | 1 cut(s) | 857 |
| HpyAV | CCTTC | 6 cut(s) | 50, 521, 522, 653, 934, 1184 |
| HpyCH4III | ACNGT | 3 cut(s) | 583, 1024, 1134 |
| HpyCH4IV | ACGT | 1 cut(s) | 677 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 259, 761, 770, 920, 929, 971 |
| HpyF3I | CTNAG | 3 cut(s) | 301, 790, 886 |
| HpySE526I | ACGT | 1 cut(s) | 677 |
| Hsp92I | GRCGYC | 1 cut(s) | 852 |
| Hsp92II | CATG | 6 cut(s) | 61, 133, 194, 668, 764, 1014 |
| HspAI | GCGC | 1 cut(s) | 921 |
| KpnI | GGTACC | 1 cut(s) | 214 |
| Kzo9I | GATC | 3 cut(s) | 30, 668, 1193 |
| LmnI | GCTCC | 4 cut(s) | 106, 929, 1012, 1028 |
| Lsp1109I | GCAGC | 6 cut(s) | 107, 110, 292, 408, 436, 811 |
| LweI | GCATC | 1 cut(s) | 15 |
| MaeI | CTAG | 2 cut(s) | 74, 113 |
| MaeII | ACGT | 1 cut(s) | 677 |
| MaeIII | GTNAC | 2 cut(s) | 69, 1184 |
| MalI | GATC | 3 cut(s) | 32, 670, 1195 |
| MbiI | CCGCTC | 1 cut(s) | 365 |
| MboI | GATC | 3 cut(s) | 30, 668, 1193 |
| MboII | GAAGA | 3 cut(s) | 206, 344, 476 |
| MfeI | CAATTG | 1 cut(s) | 386 |
| MflI | RGATCY | 2 cut(s) | 668, 1193 |
| MhlI | GDGCHC | 2 cut(s) | 1037, 1159 |
| MlsI | TGGCCA | 1 cut(s) | 731 |
| MluCI | AATT | 8 cut(s) | 169, 379, 386, 480, 697, 774, 909, 933 |
| MluNI | TGGCCA | 1 cut(s) | 731 |
| MmeI | TCCRAC | 1 cut(s) | 1006 |
| MnlI | CCTC | 9 cut(s) | 316, 552, 632, 654, 686, 768, 790, 990, 1149 |
| Mox20I | TGGCCA | 1 cut(s) | 731 |
| Mph1103I | ATGCAT | 1 cut(s) | 280 |
| MscI | TGGCCA | 1 cut(s) | 731 |
| MseI | TTAA | 2 cut(s) | 618, 1201 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1164 |
| Msp20I | TGGCCA | 1 cut(s) | 731 |
| MspA1I | CMGCKG | 3 cut(s) | 689, 957, 965 |
| MspI | CCGG | 1 cut(s) | 161 |
| MunI | CAATTG | 1 cut(s) | 386 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 259, 761, 770, 920, 929, 971 |
| NcoI | CCATGG | 2 cut(s) | 664, 1010 |
| NdeII | GATC | 3 cut(s) | 30, 668, 1193 |
| NheI | GCTAGC | 1 cut(s) | 112 |
| NlaIII | CATG | 6 cut(s) | 61, 133, 194, 668, 764, 1014 |
| NlaIV | GGNNCC | 5 cut(s) | 108, 212, 607, 712, 756 |
| NmuCI | GTSAC | 2 cut(s) | 69, 1184 |
| NsiI | ATGCAT | 1 cut(s) | 280 |
| NspI | RCATGY | 1 cut(s) | 133 |
| PaeI | GCATGC | 1 cut(s) | 133 |
| PaeR7I | CTCGAG | 1 cut(s) | 254 |
| PaqCI | CACCTGC | 1 cut(s) | 1142 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1028 |
| PfeI | GAWTC | 5 cut(s) | 809, 945, 1015, 1097, 1169 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 289 |
| PkrI | GCNGC | 7 cut(s) | 97, 100, 111, 307, 423, 426, 826 |
| PspN4I | GGNNCC | 5 cut(s) | 108, 212, 607, 712, 756 |
| PspPI | GGNCC | 3 cut(s) | 449, 641, 977 |
| PstI | CTGCAG | 1 cut(s) | 426 |
| PstNI | CAGNNNCTG | 2 cut(s) | 83, 746 |
| PsuI | RGATCY | 2 cut(s) | 668, 1193 |
| PvuII | CAGCTG | 3 cut(s) | 689, 957, 965 |
| RsaI | GTAC | 2 cut(s) | 212, 534 |
| RsaNI | GTAC | 2 cut(s) | 211, 533 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1164 |
| SaqAI | TTAA | 2 cut(s) | 618, 1201 |
| SatI | GCNGC | 7 cut(s) | 96, 99, 110, 306, 422, 425, 825 |
| Sau3AI | GATC | 3 cut(s) | 30, 668, 1193 |
| Sau96I | GGNCC | 3 cut(s) | 449, 641, 977 |
| SduI | GDGCHC | 2 cut(s) | 1037, 1159 |
| SfaNI | GCATC | 1 cut(s) | 15 |
| SfcI | CTRYAG | 1 cut(s) | 422 |
| Sfr274I | CTCGAG | 1 cut(s) | 254 |
| SlaI | CTCGAG | 1 cut(s) | 254 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1164 |
| SmlI | CTYRAG | 1 cut(s) | 254 |
| SmoI | CTYRAG | 1 cut(s) | 254 |
| SphI | GCATGC | 1 cut(s) | 133 |
| Sse9I | AATT | 8 cut(s) | 169, 379, 386, 480, 697, 774, 909, 933 |
| SsiI | CCGC | 3 cut(s) | 110, 320, 363 |
| SspMI | CTAG | 2 cut(s) | 74, 113 |
| StyI | CCWWGG | 2 cut(s) | 664, 1010 |
| TaaI | ACNGT | 3 cut(s) | 583, 1024, 1134 |
| TaiI | ACGT | 1 cut(s) | 680 |
| TaqI | TCGA | 2 cut(s) | 255, 855 |
| TasI | AATT | 8 cut(s) | 169, 379, 386, 480, 697, 774, 909, 933 |
| TauI | GCSGC | 1 cut(s) | 112 |
| TfiI | GAWTC | 5 cut(s) | 809, 945, 1015, 1097, 1169 |
| Tru1I | TTAA | 2 cut(s) | 618, 1201 |
| Tru9I | TTAA | 2 cut(s) | 618, 1201 |
| TscAI | CASTG | 3 cut(s) | 580, 721, 841 |
| TseFI | GTSAC | 2 cut(s) | 69, 1184 |
| TseI | GCWGC | 6 cut(s) | 95, 98, 305, 421, 424, 824 |
| Tsp45I | GTSAC | 2 cut(s) | 69, 1184 |
| TspDTI | ATGAA | 8 cut(s) | 46, 102, 207, 504, 540, 672, 1017, 1161 |
| TspRI | CASTG | 3 cut(s) | 580, 721, 841 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 289 |
| VneI | GTGCAC | 1 cut(s) | 1155 |
| XapI | RAATTY | 4 cut(s) | 169, 379, 480, 697 |
| XceI | RCATGY | 1 cut(s) | 133 |
| XhoI | CTCGAG | 1 cut(s) | 254 |
| XspI | CTAG | 2 cut(s) | 74, 113 |
| Zsp2I | ATGCAT | 1 cut(s) | 280 |