Rroxscaffold_3G00256230

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000302
Physical Location & Seq
Forward (+)
50622276 .. 50624573
2298 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00256230.1

Sequence Viewer

Length: 2298 bp
ATGGAGAACAAAACGTGTGTAGTGATGCAGATCATATACCTTCTTGGTTTTAGTTTCATGTTTTACATGTCACTAGCAGTTGCTGATGAAAAAGTGGCAGCATTGGAGCCGCTAGCCAAGACAAAAAGCATGCAAACTTACATAGTTTGGGTGGAAAAGCCAGTCAGCGATTTGATTGCACAATCTGAACATGAAGATTTAGGGAGTTGGTACCAGACATTTTTGCCCCAAACTATTGCAAACTCAAACCAGCTCGAGAAGCCACGAATGGTTTATGCGTACCACAATGTGGCAACTGGCTTCGCAGCAAAGCTGACAGCGGAGGAAGTTGAAGAGATGAAAAAGAAAGATGGGTTTATCTCAGCTCATCCAGAACGAAATCTGCAATTGCACACAACTCATAGTCCTAACTTCTTGGGGCTGCAGCAAGGAATGGGACTTGGTAAAGGGCCTAACTATGGTGAAGGTGTGATTATTGGAATTTTGGATAGTGGGATATTCCCAGACCATCCTTCATTTAGTGATGAAGGAGTACCACCTCCTCCAGCTAAATGGAAAGGCAAGTGTGATTTCAGCGGGACAGTCTGCAATAACAAGCTTGTCGGTGCCAGAAGTTTTAATGGTGGAAAAACAACTGGAGGCCCTCCAGTTGACGATGAAGGCCATGGAACTCACACGTCAAGCACAGCTGGAGGAAATTTTGTGAAAGGTGCCAGCGTGTTTGGAATGGCCAACGGCACAGCAACTGGCATGGCACCTCATGCTCACTTGGCAGTGTACAAAGTATGCTCAGACGAGCGTTGTTCTGAATCAGACATTATAGCTGCTATGGACGCTGCTGTTGATGATGGCGTGGATGTGCTTTCCCTTTCACTCGGTGGTGGCTCAGCTTATTTTTATGCTGATGGAATTGCAGTCGGCGCATTTGGAGCAATTCAGAAGGGAGTCTTTGTCAGCTGTTCAGCTGGAAATGCTGGCCCTGACTATATGACTTTATCCAATGAAGCTCCATGGATTCTCACGGTTGGAGCAAGCACCATTGACAGAAGCATAAGAGCAACAGCAAAGCTTGGAAATGGGCAACAATATGACGGGGAATCACTATTCCAGCCTAAAGATTTTGGTTCAAAACTGTTGCCTCTTGTTTATGCAGGTGCACATAGCAATGATTCATCAGCTATATGTGACGAAGGATCTCTTGAAAATGTTGAAGGGAAAGTAGTGGTCTGTGAGCTAGGTGGAGGAATTGGAAGAATTGCAAAAGGGGTAGAAGTAAAAAGAGCTGGTGGTGTTGCCATGATTCTTGTCAACCCAGACTTTGCTGGCTATACCACCATAGCAGAAGCTCACGTGCTTCCGGCAACACATGTGAGTTACGCTGCAGGGGTGAGTATCAAAAGCTATATAAATTCAACCTCAACTCCTACAGCGACAATCTTATTCAAAGGTACTGTCATCGGTGATCAGCTTGCTCCCAAGGTTGCTTTCTTTTCATCAAGAGGACCAAGCCTGGCAAGCCCTGCAATTTTGAAACCTGACATTATTGGTCCCGGTGTGAGCATCCTAGCTGCGTGGCCTTTTTCGGTGGATAATGCCACAGAATCTAAGGCCACATTTGACATTATTTCAGGTACTTCAATGTCATGCCCTCACCTAAGTGGCATTGCAGCCTTGCTCAAAAGCTCACACCCTGACTGGTCACCTGCTGCCATTAAGTCTGCAATGATGACAACTGCTGAAGTGAACAACCTCGCTGGCTCGGCCATTCTTGATCAAACACTTCTTGCAGCAGACCTCTTTGCCATCGGCGCAGGCCATGTTAACCCTTCAAAAGCAAATGACCCTGGGCTCATCTACGACATACAACCAGAGGACTACATTCCTTACTTGTGTGGTTTGAATTACACAAGCAAACAGATAGCGGCAATCACCCAACAAAAAGTGACGTGCTCTAAAGTAGGAGTCATACCAGAAGCACAGCTAAACTATCCCACATTTTGTATCTATATAGAGTCTGGTGGTGAGCCTCAGAATTACACAAGAACCTTGACCAATGTTGGCCCAGCTAATTCAACTTACGAATTGGCTCCCCTGAGACTACATAAAATGGACATCACTGTGCTACCTGAGGTGCTTACATTTACAGAGGTTAACCAGAAATTGACATACCGTGTGGTGTTTGCCGCACAAGACGGAGCTGGGAAAGATGGTATACCATTTTCTCAGGGATATTTGAGTTGGGTGTCTCATCAGCATTCTGTTAACAGCCAAATATCTGTGGTCTTTGACTATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

765

Amino Acids

80.89

Weight (kDa)

5.31

Isoelectric Point (pI)

30.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 46 - 131 2.9e-11 Peptidase inhibitor I9
Peptidase_S8 PF00082 154 - 582 4.2e-47 Subtilase family
PA PF02225 380 - 464 1.3e-10 PA domain
fn3_6 PF17766 660 - 760 5.2e-21 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1142, 1711
Acc36I ACCTGC 2 cut(s) 1142, 1711
Acc65I GGTACC 1 cut(s) 210
AccB1I GGYRCC 4 cut(s) 210, 605, 710, 754
AccB7I CCANNNNNTGG 1 cut(s) 289
AccI GTMKAC 1 cut(s) 2212
AciI CCGC 5 cut(s) 110, 320, 576, 1922, 2184
AclWI GGATC 1 cut(s) 1201
AcoI YGGCCR 2 cut(s) 729, 1761
AcsI RAATTY 3 cut(s) 480, 697, 1408
AcuI CTGAAG 1 cut(s) 1758
AcvI CACGTG 1 cut(s) 1351
AdeI CACNNNGTG 2 cut(s) 289, 878
AfaI GTAC 6 cut(s) 212, 281, 534, 779, 1450, 1633
AfiI CCNNNNNNNGG 2 cut(s) 289, 458
AflIII ACRYGT 4 cut(s) 14, 66, 675, 1366
AjiI CACGTC 2 cut(s) 678, 1947
AjnI CCWGG 2 cut(s) 1509, 1843
AjuI GAANNNNNNNTTGG 2 cut(s) 1469, 1501
AleI CACNNNNGTG 1 cut(s) 1656
Alw21I GWGCWC 2 cut(s) 1159, 1952
Alw26I GTCTC 2 cut(s) 2089, 2250
Alw44I GTGCAC 1 cut(s) 1155
AlwI GGATC 1 cut(s) 1201
AlwNI CAGNNNCTG 2 cut(s) 83, 746
Ama87I CYCGRG 1 cut(s) 254
ApaLI GTGCAC 1 cut(s) 1155
ApoI RAATTY 3 cut(s) 480, 697, 1408
ArsI GACNNNNNNTTYG 2 cut(s) 1437, 1469
Asp718I GGTACC 1 cut(s) 210
AspLEI GCGC 2 cut(s) 923, 1811
AspS9I GGNCC 6 cut(s) 449, 641, 977, 1502, 1547, 2060
AsuC2I CCSGG 1 cut(s) 1551
AsuHPI GGTGA 7 cut(s) 473, 1399, 1472, 1643, 1692, 1921, 2033
AsuNHI GCTAGC 1 cut(s) 112
AvaI CYCGRG 1 cut(s) 254
AvaII GGWCC 2 cut(s) 1502, 1547
AxyI CCTNAGG 1 cut(s) 2127
BaeGI GKGCMC 1 cut(s) 1159
BalI TGGCCA 1 cut(s) 731
BanI GGYRCC 4 cut(s) 210, 605, 710, 754
BanII GRGCYC 1 cut(s) 1851
BbrPI CACGTG 1 cut(s) 1351
Bbv12I GWGCWC 2 cut(s) 1159, 1952
BccI CCATC 6 cut(s) 344, 516, 842, 899, 1811, 2201
BceAI ACGGC 1 cut(s) 751
BcgI CGANNNNNNTGC 2 cut(s) 158, 192
BciT130I CCWGG 2 cut(s) 1511, 1845
BclI TGATCA 2 cut(s) 1462, 1771
BcnI CCSGG 1 cut(s) 1551
BcoDI GTCTC 2 cut(s) 2089, 2250
BfaI CTAG 4 cut(s) 74, 113, 1235, 1565
BfmI CTRYAG 3 cut(s) 422, 1380, 1425
BfuAI ACCTGC 2 cut(s) 1142, 1711
BlpI GCTNAGC 1 cut(s) 886
Bme1390I CCNGG 3 cut(s) 1511, 1551, 1845
Bme18I GGWCC 2 cut(s) 1502, 1547
BmeT110I CYCGRG 1 cut(s) 254
BmgBI CACGTC 2 cut(s) 678, 1947
BmgT120I GGNCC 6 cut(s) 449, 641, 977, 1502, 1547, 2060
BmiI GGNNCC 7 cut(s) 108, 212, 607, 712, 756, 1549, 2088
BmrFI CCNGG 3 cut(s) 1511, 1551, 1845
BmsI GCATC 2 cut(s) 15, 1569
BmtI GCTAGC 1 cut(s) 116
BpmI CTGGAG 4 cut(s) 528, 630, 657, 711
Bpu1102I GCTNAGC 1 cut(s) 886
BpuMI CCSGG 1 cut(s) 1551
BsaAI YACGTR 1 cut(s) 1351
BsaBI GATNNNNATC 1 cut(s) 29
BsaJI CCNNGG 5 cut(s) 664, 1010, 1476, 1843, 1844
BsaXI ACNNNNNCTCC 4 cut(s) 936, 966, 1405, 1435
Bsc4I CCNNNNNNNGG 2 cut(s) 289, 458
Bse1I ACTGG 6 cut(s) 161, 301, 640, 647, 751, 1700
Bse21I CCTNAGG 1 cut(s) 2127
Bse3DI GCAATG 3 cut(s) 1171, 1662, 1728
Bse8I GATNNNNATC 1 cut(s) 29
BseBI CCWGG 2 cut(s) 1511, 1845
BseDI CCNNGG 5 cut(s) 664, 1010, 1476, 1843, 1844
BseGI GGATG 4 cut(s) 367, 508, 862, 1560
BseJI GATNNNNATC 1 cut(s) 29
BseLI CCNNNNNNNGG 2 cut(s) 289, 458
BseMI GCAATG 3 cut(s) 1171, 1662, 1728
BseMII CTCAG 7 cut(s) 375, 804, 900, 2042, 2084, 2118, 2237
BseNI ACTGG 6 cut(s) 161, 301, 640, 647, 751, 1700
BseRI GAGGAG 1 cut(s) 531
BseSI GKGCMC 1 cut(s) 1159
BseYI CCCAGC 2 cut(s) 2062, 2198
BshNI GGYRCC 4 cut(s) 210, 605, 710, 754
BsiHKAI GWGCWC 2 cut(s) 1159, 1952
BsiHKCI CYCGRG 1 cut(s) 254
BsiSI CCGG 2 cut(s) 1358, 1551
BslFI GGGAC 3 cut(s) 450, 592, 1533
BslI CCNNNNNNNGG 2 cut(s) 289, 458
BsmAI GTCTC 2 cut(s) 2089, 2250
BsmFI GGGAC 3 cut(s) 450, 592, 1533
BsmI GAATGC 1 cut(s) 2254
BsoBI CYCGRG 1 cut(s) 254
Bsp1286I GDGCHC 3 cut(s) 1159, 1851, 1952
Bsp1407I TGTACA 1 cut(s) 777
Bsp143I GATC 4 cut(s) 30, 1193, 1462, 1771
Bsp1720I GCTNAGC 1 cut(s) 886
Bsp19I CCATGG 2 cut(s) 664, 1010
BspACI CCGC 5 cut(s) 110, 320, 576, 1922, 2184
BspCNI CTCAG 7 cut(s) 374, 803, 899, 2041, 2085, 2119, 2236
BspLI GGNNCC 7 cut(s) 108, 212, 607, 712, 756, 1549, 2088
BspMAI CTGCAG 2 cut(s) 426, 1384
BspMI ACCTGC 2 cut(s) 1142, 1711
BspOI GCTAGC 1 cut(s) 116
BspPI GGATC 1 cut(s) 1201
BspT107I GGYRCC 4 cut(s) 210, 605, 710, 754
BsrDI GCAATG 3 cut(s) 1171, 1662, 1728
BsrGI TGTACA 1 cut(s) 777
BsrI ACTGG 6 cut(s) 161, 301, 640, 647, 751, 1700
BssECI CCNNGG 5 cut(s) 664, 1010, 1476, 1843, 1844
BssMI GATC 4 cut(s) 30, 1193, 1462, 1771
BssNAI GTATAC 1 cut(s) 2213
BssT1I CCWWGG 3 cut(s) 664, 1010, 1476
Bst1107I GTATAC 1 cut(s) 2213
Bst2UI CCWGG 2 cut(s) 1511, 1845
Bst4CI ACNGT 6 cut(s) 583, 1024, 1134, 1453, 2119, 2171
Bst6I CTCTTC 1 cut(s) 327
BstAPI GCANNNNNTGC 2 cut(s) 761, 1520
BstAUI TGTACA 1 cut(s) 777
BstBAI YACGTR 1 cut(s) 1351
BstDEI CTNAG 9 cut(s) 361, 790, 886, 1605, 1655, 2028, 2093, 2127, 2223
BstDSI CCRYGG 2 cut(s) 664, 1010
BstEII GGTNACC 1 cut(s) 1698
BstF5I GGATG 4 cut(s) 367, 508, 862, 1560
BstHHI GCGC 2 cut(s) 923, 1811
BstKTI GATC 4 cut(s) 33, 1196, 1465, 1774
BstMAI GTCTC 2 cut(s) 2089, 2250
BstMBI GATC 4 cut(s) 30, 1193, 1462, 1771
BstNI CCWGG 2 cut(s) 1511, 1845
BstNSI RCATGY 3 cut(s) 70, 133, 1370
BstPI GGTNACC 1 cut(s) 1698
BstSCI CCNGG 3 cut(s) 1509, 1549, 1843
BstSFI CTRYAG 3 cut(s) 422, 1380, 1425
BstSLI GKGCMC 1 cut(s) 1159
BstX2I RGATCY 1 cut(s) 1193
BstXI CCANNNNNNTGG 1 cut(s) 552
BstYI RGATCY 1 cut(s) 1193
BstZ17I GTATAC 1 cut(s) 2213
Bsu36I CCTNAGG 1 cut(s) 2127
BtgI CCRYGG 2 cut(s) 664, 1010
BtrI CACGTC 2 cut(s) 678, 1947
BtsCI GGATG 4 cut(s) 367, 508, 862, 1560
BtsI GCAGTG 1 cut(s) 780
BtsIMutI CAGTG 2 cut(s) 780, 2115
BveI ACCTGC 2 cut(s) 1142, 1711
CaiI CAGNNNCTG 2 cut(s) 83, 746
CfoI GCGC 2 cut(s) 923, 1811
Cfr13I GGNCC 6 cut(s) 449, 641, 977, 1502, 1547, 2060
CseI GACGC 1 cut(s) 842
Csp6I GTAC 6 cut(s) 211, 280, 533, 778, 1449, 1632
CviQI GTAC 6 cut(s) 211, 280, 533, 778, 1449, 1632
DdeI CTNAG 9 cut(s) 361, 790, 886, 1605, 1655, 2028, 2093, 2127, 2223
DpnI GATC 4 cut(s) 32, 1195, 1464, 1773
DpnII GATC 4 cut(s) 30, 1193, 1462, 1771
DraIII CACNNNGTG 2 cut(s) 289, 878
EaeI YGGCCR 2 cut(s) 729, 1761
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
Eco130I CCWWGG 3 cut(s) 664, 1010, 1476
Eco24I GRGCYC 1 cut(s) 1851
Eco47I GGWCC 2 cut(s) 1502, 1547
Eco57I CTGAAG 1 cut(s) 1758
Eco72I CACGTG 1 cut(s) 1351
Eco81I CCTNAGG 1 cut(s) 2127
Eco88I CYCGRG 1 cut(s) 254
Eco91I GGTNACC 1 cut(s) 1698
EcoO109I RGGNCCY 2 cut(s) 449, 641
EcoO65I GGTNACC 1 cut(s) 1698
EcoRII CCWGG 2 cut(s) 1509, 1843
EcoT14I CCWWGG 3 cut(s) 664, 1010, 1476
EcoT38I GRGCYC 1 cut(s) 1851
ErhI CCWWGG 3 cut(s) 664, 1010, 1476
FalI AAGNNNNNCTT 4 cut(s) 932, 964, 1182, 1214
FaqI GGGAC 3 cut(s) 450, 592, 1533
FauI CCCGC 1 cut(s) 569
FbaI TGATCA 2 cut(s) 1462, 1771
FblI GTMKAC 1 cut(s) 2212
FokI GGATG 4 cut(s) 354, 495, 869, 1547
FriOI GRGCYC 1 cut(s) 1851
FspBI CTAG 4 cut(s) 74, 113, 1235, 1565
GlaI GCGC 2 cut(s) 922, 1810
GsaI CCCAGC 2 cut(s) 2066, 2202
GsuI CTGGAG 4 cut(s) 528, 630, 657, 711
HapII CCGG 2 cut(s) 1358, 1551
HgaI GACGC 1 cut(s) 842
HhaI GCGC 2 cut(s) 923, 1811
Hin6I GCGC 2 cut(s) 921, 1809
HinP1I GCGC 2 cut(s) 921, 1809
HincII GTYRAC 5 cut(s) 652, 1309, 1822, 2152, 2263
HindII GTYRAC 5 cut(s) 652, 1309, 1822, 2152, 2263
HindIII AAGCTT 2 cut(s) 596, 1067
HinfI GANTC 9 cut(s) 809, 945, 1015, 1097, 1169, 1300, 1601, 1962, 2012
HpaI GTTAAC 3 cut(s) 1822, 2152, 2263
HpaII CCGG 2 cut(s) 1358, 1551
HphI GGTGA 7 cut(s) 473, 1399, 1472, 1643, 1692, 1921, 2033
Hpy166II GTNNAC 9 cut(s) 652, 778, 1157, 1309, 1744, 1822, 2152, 2213, 2263
Hpy188I TCNGA 6 cut(s) 187, 793, 808, 814, 939, 2031
Hpy188III TCNNGA 5 cut(s) 256, 371, 1199, 1497, 1769
Hpy8I GTNNAC 9 cut(s) 652, 778, 1157, 1309, 1744, 1822, 2152, 2213, 2263
HpyAV CCTTC 9 cut(s) 50, 458, 521, 522, 653, 934, 1184, 1205, 1836
HpyCH4III ACNGT 6 cut(s) 583, 1024, 1134, 1453, 2119, 2171
HpyCH4IV ACGT 4 cut(s) 14, 677, 1350, 1946
HpyF3I CTNAG 9 cut(s) 361, 790, 886, 1605, 1655, 2028, 2093, 2127, 2223
HpySE526I ACGT 4 cut(s) 14, 677, 1350, 1946
HspAI GCGC 2 cut(s) 921, 1809
KpnI GGTACC 1 cut(s) 214
Ksp22I TGATCA 2 cut(s) 1462, 1771
KspAI GTTAAC 3 cut(s) 1822, 2152, 2263
Kzo9I GATC 4 cut(s) 30, 1193, 1462, 1771
LmnI GCTCC 7 cut(s) 106, 929, 1012, 1028, 1477, 2092, 2195
LweI GCATC 2 cut(s) 15, 1569
MaeI CTAG 4 cut(s) 74, 113, 1235, 1565
MaeII ACGT 4 cut(s) 14, 677, 1350, 1946
MaeIII GTNAC 5 cut(s) 69, 1184, 1373, 1698, 1942
MalI GATC 4 cut(s) 32, 1195, 1464, 1773
MboI GATC 4 cut(s) 30, 1193, 1462, 1771
MboII GAAGA 3 cut(s) 206, 344, 1263
MfeI CAATTG 1 cut(s) 386
MflI RGATCY 1 cut(s) 1193
MhlI GDGCHC 3 cut(s) 1159, 1851, 1952
MlsI TGGCCA 1 cut(s) 731
MluNI TGGCCA 1 cut(s) 731
MlyI GAGTC 3 cut(s) 954, 1971, 2021
MmeI TCCRAC 1 cut(s) 1006
Mox20I TGGCCA 1 cut(s) 731
MscI TGGCCA 1 cut(s) 731
MseI TTAA 5 cut(s) 618, 1713, 1821, 2151, 2262
MslI CAYNNNNRTG 3 cut(s) 1164, 1656, 2117
Msp20I TGGCCA 1 cut(s) 731
MspA1I CMGCKG 5 cut(s) 320, 576, 689, 957, 965
MspI CCGG 2 cut(s) 1358, 1551
MspR9I CCNGG 3 cut(s) 1511, 1551, 1845
MunI CAATTG 1 cut(s) 386
Mva1269I GAATGC 1 cut(s) 2254
MvaI CCWGG 2 cut(s) 1511, 1845
NciI CCSGG 1 cut(s) 1551
NcoI CCATGG 2 cut(s) 664, 1010
NdeII GATC 4 cut(s) 30, 1193, 1462, 1771
NheI GCTAGC 1 cut(s) 112
NlaIV GGNNCC 7 cut(s) 108, 212, 607, 712, 756, 1549, 2088
NmeAIII GCCGAG 1 cut(s) 1739
NmuCI GTSAC 4 cut(s) 69, 1184, 1698, 1942
NspI RCATGY 3 cut(s) 70, 133, 1370
OliI CACNNNNGTG 1 cut(s) 1656
PaeI GCATGC 1 cut(s) 133
PaeR7I CTCGAG 1 cut(s) 254
PaqCI CACCTGC 2 cut(s) 1142, 1711
PasI CCCWGGG 1 cut(s) 1844
PciI ACATGT 2 cut(s) 66, 1366
PctI GAATGC 1 cut(s) 2254
PfeI GAWTC 6 cut(s) 809, 1015, 1097, 1169, 1300, 1601
PflMI CCANNNNNTGG 1 cut(s) 289
PleI GAGTC 3 cut(s) 953, 1970, 2020
PmaCI CACGTG 1 cut(s) 1351
PmlI CACGTG 1 cut(s) 1351
PpsI GAGTC 3 cut(s) 953, 1970, 2020
Ppu21I YACGTR 1 cut(s) 1351
PscI ACATGT 2 cut(s) 66, 1366
Psp6I CCWGG 2 cut(s) 1509, 1843
PspCI CACGTG 1 cut(s) 1351
PspEI GGTNACC 1 cut(s) 1698
PspFI CCCAGC 2 cut(s) 2062, 2198
PspGI CCWGG 2 cut(s) 1509, 1843
PspN4I GGNNCC 7 cut(s) 108, 212, 607, 712, 756, 1549, 2088
PspPI GGNCC 6 cut(s) 449, 641, 977, 1502, 1547, 2060
PstI CTGCAG 2 cut(s) 426, 1384
PstNI CAGNNNCTG 2 cut(s) 83, 746
PsuI RGATCY 1 cut(s) 1193
PvuII CAGCTG 3 cut(s) 689, 957, 965
RsaI GTAC 6 cut(s) 212, 281, 534, 779, 1450, 1633
RsaNI GTAC 6 cut(s) 211, 280, 533, 778, 1449, 1632
RseI CAYNNNNRTG 3 cut(s) 1164, 1656, 2117
SaqAI TTAA 5 cut(s) 618, 1713, 1821, 2151, 2262
Sau3AI GATC 4 cut(s) 30, 1193, 1462, 1771
Sau96I GGNCC 6 cut(s) 449, 641, 977, 1502, 1547, 2060
SchI GAGTC 3 cut(s) 954, 1971, 2021
ScrFI CCNGG 3 cut(s) 1511, 1551, 1845
SduI GDGCHC 3 cut(s) 1159, 1851, 1952
SfaNI GCATC 2 cut(s) 15, 1569
SfcI CTRYAG 3 cut(s) 422, 1380, 1425
Sfr274I CTCGAG 1 cut(s) 254
SinI GGWCC 2 cut(s) 1502, 1547
SlaI CTCGAG 1 cut(s) 254
SmiMI CAYNNNNRTG 3 cut(s) 1164, 1656, 2117
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
SphI GCATGC 1 cut(s) 133
SsiI CCGC 5 cut(s) 110, 320, 576, 1922, 2184
SspMI CTAG 4 cut(s) 74, 113, 1235, 1565
StyD4I CCNGG 3 cut(s) 1509, 1549, 1843
StyI CCWWGG 3 cut(s) 664, 1010, 1476
TaaI ACNGT 6 cut(s) 583, 1024, 1134, 1453, 2119, 2171
TaiI ACGT 4 cut(s) 17, 680, 1353, 1949
TaqI TCGA 1 cut(s) 255
TatI WGTACW 1 cut(s) 777
TauI GCSGC 3 cut(s) 112, 1925, 2186
TfiI GAWTC 6 cut(s) 809, 1015, 1097, 1169, 1300, 1601
Tru1I TTAA 5 cut(s) 618, 1713, 1821, 2151, 2262
Tru9I TTAA 5 cut(s) 618, 1713, 1821, 2151, 2262
TscAI CASTG 2 cut(s) 780, 2122
TseFI GTSAC 4 cut(s) 69, 1184, 1698, 1942
Tsp45I GTSAC 4 cut(s) 69, 1184, 1698, 1942
TspGWI ACGGA 1 cut(s) 2208
TspRI CASTG 2 cut(s) 780, 2122
Van91I CCANNNNNTGG 1 cut(s) 289
VneI GTGCAC 1 cut(s) 1155
VpaK11BI GGWCC 2 cut(s) 1502, 1547
XapI RAATTY 3 cut(s) 480, 697, 1408
XceI RCATGY 3 cut(s) 70, 133, 1370
XhoI CTCGAG 1 cut(s) 254
XmiI GTMKAC 1 cut(s) 2212
XspI CTAG 4 cut(s) 74, 113, 1235, 1565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.