Rh7CG193200

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0000302
Physical Location & Seq
Reverse (-)
17039550 .. 17040347
798 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG193200.1

Sequence Viewer

Length: 798 bp
ATGAAGCAACGAATGGTTTATGCATACCACCATGTGGCGACTGGCTTTGCAGCAAAGTTGACAGCAGAGGAAGTAAAAGAAATAGAGACAAAAGATGGGTTTATGCCTGCTCATCTAGATAGGATTCTGCCTTTGCACACTACCCATAGTCCGAACTTCTTGGGGTTATACCAAGGATTGGGACTTTGGAAAGGGTCTAATTATGGTGAAGGTGTAACTATTGGAGTTTTGGATTCTGGGATAACACCAGGTCATCCCTCATTCAGCGATGAAGGAGTGCCACCTCCTCCGGCTAAATGGAAAGGTAAGTGCTATTTCAACGGGACATTCTGCAATAACAAGCTTATTGGTGCCAGAAGTTTCCGAGGTGGGCAAACTACTGGAGATCCACCAGTTGATGTTGATGGCCATGGTACCCACACATCCAACACAGCCGGAGGAAACTTTGTGAAAGGCGCAAACGCGTTTGGAATGGCAAGTGGAACAATGTCTAGCATGGCACCTTATGCTTACTTGGCAAAGTACCAAGTTTGTTATGATCACGGTTGTTCTGAAAGTGACATTTTAGCTGCTATGGACACTGCTGTTGCCGATGGAGTGGACGTGCTCTCCTCTCACTTGGTGGTGGTTCAGCTGCTTTCTATTATGATGGAATCGCAGTTGGTGCATTTGGAGCAATTCAGAAGGGAGTTTTTGTCAGCTGCTCAGCAGGAAATGAGCGTCCTGTTTATGGTTCATTGTCCAATGAAGCCCCATGGATTCCAACTGTTGGAGCAAGCACTATTGACAGAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

265

Amino Acids

28.67

Weight (kDa)

5.97

Isoelectric Point (pI)

36.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 3 - 46 3.6e-06 Peptidase inhibitor I9
Peptidase_S8 PF00082 69 - 204 2.1e-13 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 413
AccB1I GGYRCC 3 cut(s) 350, 413, 499
AccB7I CCANNNNNTGG 3 cut(s) 34, 178, 769
AccII CGCG 1 cut(s) 464
AclWI GGATC 1 cut(s) 380
AcoI YGGCCR 1 cut(s) 406
AdeI CACNNNGTG 2 cut(s) 34, 622
AfaI GTAC 2 cut(s) 415, 524
AfiI CCNNNNNNNGG 4 cut(s) 34, 178, 730, 769
AflIII ACRYGT 1 cut(s) 462
AgsI TTSAA 1 cut(s) 319
AjiI CACGTC 1 cut(s) 604
AjnI CCWGG 1 cut(s) 247
AluBI AGCT 4 cut(s) 343, 569, 634, 701
AluI AGCT 4 cut(s) 343, 569, 634, 701
Alw21I GWGCWC 1 cut(s) 609
Alw26I GTCTC 1 cut(s) 80
AlwI GGATC 1 cut(s) 380
AoxI GGCC 1 cut(s) 406
ApeKI GCWGC 4 cut(s) 50, 569, 634, 701
Asp718I GGTACC 1 cut(s) 413
AspLEI GCGC 1 cut(s) 458
AsuHPI GGTGA 1 cut(s) 218
BalI TGGCCA 1 cut(s) 408
BanI GGYRCC 3 cut(s) 350, 413, 499
Bbv12I GWGCWC 1 cut(s) 609
BbvI GCAGC 4 cut(s) 62, 556, 621, 688
BccI CCATC 4 cut(s) 89, 398, 587, 643
BciT130I CCWGG 1 cut(s) 249
BclI TGATCA 1 cut(s) 538
BcoDI GTCTC 1 cut(s) 80
BfaI CTAG 2 cut(s) 116, 492
BisI GCNGC 4 cut(s) 51, 570, 635, 702
BlpI GCTNAGC 1 cut(s) 705
BlsI GCNGC 4 cut(s) 52, 571, 636, 703
Bme1390I CCNGG 1 cut(s) 249
BmgBI CACGTC 1 cut(s) 604
BmiI GGNNCC 3 cut(s) 352, 415, 501
BmrFI CCNGG 1 cut(s) 249
BpmI CTGGAG 1 cut(s) 402
Bpu1102I GCTNAGC 1 cut(s) 705
BsaJI CCNNGG 4 cut(s) 172, 364, 409, 754
BsaXI ACNNNNNCTCC 6 cut(s) 588, 593, 618, 623, 680, 710
Bsc4I CCNNNNNNNGG 4 cut(s) 34, 178, 730, 769
Bse1I ACTGG 3 cut(s) 46, 385, 392
BseBI CCWGG 1 cut(s) 249
BseDI CCNNGG 4 cut(s) 172, 364, 409, 754
BseGI GGATG 2 cut(s) 253, 422
BseLI CCNNNNNNNGG 4 cut(s) 34, 178, 730, 769
BseMII CTCAG 1 cut(s) 719
BseNI ACTGG 3 cut(s) 46, 385, 392
BseRI GAGGAG 2 cut(s) 276, 601
BseXI GCAGC 4 cut(s) 62, 556, 621, 688
Bsh1236I CGCG 1 cut(s) 464
BshFI GGCC 1 cut(s) 408
BshNI GGYRCC 3 cut(s) 350, 413, 499
BsiHKAI GWGCWC 1 cut(s) 609
BsiSI CCGG 2 cut(s) 290, 435
BslFI GGGAC 2 cut(s) 195, 337
BslI CCNNNNNNNGG 4 cut(s) 34, 178, 730, 769
BsmAI GTCTC 1 cut(s) 80
BsmFI GGGAC 2 cut(s) 195, 337
BsnI GGCC 1 cut(s) 408
Bsp1286I GDGCHC 1 cut(s) 609
Bsp143I GATC 2 cut(s) 385, 538
Bsp1720I GCTNAGC 1 cut(s) 705
Bsp19I CCATGG 2 cut(s) 409, 754
BspANI GGCC 1 cut(s) 408
BspCNI CTCAG 1 cut(s) 718
BspFNI CGCG 1 cut(s) 464
BspLI GGNNCC 3 cut(s) 352, 415, 501
BspPI GGATC 1 cut(s) 380
BspT107I GGYRCC 3 cut(s) 350, 413, 499
BsrI ACTGG 3 cut(s) 46, 385, 392
BssECI CCNNGG 4 cut(s) 172, 364, 409, 754
BssMI GATC 2 cut(s) 385, 538
BssT1I CCWWGG 3 cut(s) 172, 409, 754
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 2 cut(s) 545, 768
BstAPI GCANNNNNTGC 2 cut(s) 506, 664
BstC8I GCNNGC 2 cut(s) 108, 777
BstDEI CTNAG 1 cut(s) 705
BstDSI CCRYGG 2 cut(s) 409, 754
BstF5I GGATG 2 cut(s) 253, 422
BstFNI CGCG 1 cut(s) 464
BstHHI GCGC 1 cut(s) 458
BstKTI GATC 2 cut(s) 388, 541
BstMAI GTCTC 1 cut(s) 80
BstMBI GATC 2 cut(s) 385, 538
BstMWI GCNNNNNNNGC 4 cut(s) 506, 515, 664, 673
BstNI CCWGG 1 cut(s) 249
BstSCI CCNGG 1 cut(s) 247
BstUI CGCG 1 cut(s) 464
BstV1I GCAGC 4 cut(s) 62, 556, 621, 688
BstX2I RGATCY 1 cut(s) 385
BstYI RGATCY 1 cut(s) 385
BsuRI GGCC 1 cut(s) 408
BtgI CCRYGG 2 cut(s) 409, 754
BtgZI GCGATG 1 cut(s) 282
BtrI CACGTC 1 cut(s) 604
BtsCI GGATG 2 cut(s) 253, 422
BtsI GCAGTG 1 cut(s) 579
BtsIMutI CAGTG 1 cut(s) 579
Cac8I GCNNGC 2 cut(s) 108, 777
CfoI GCGC 1 cut(s) 458
CseI GACGC 1 cut(s) 709
CsiI ACCWGGT 1 cut(s) 247
Csp6I GTAC 2 cut(s) 414, 523
CviAII CATG 4 cut(s) 32, 410, 496, 755
CviJI RGCY 9 cut(s) 45, 293, 343, 408, 434, 569, 634, 701, 751
CviKI_1 RGCY 9 cut(s) 45, 293, 343, 408, 434, 569, 634, 701, 751
CviQI GTAC 2 cut(s) 414, 523
DdeI CTNAG 1 cut(s) 705
DpnI GATC 2 cut(s) 387, 540
DpnII GATC 2 cut(s) 385, 538
DraIII CACNNNGTG 2 cut(s) 34, 622
EaeI YGGCCR 1 cut(s) 406
Eco130I CCWWGG 3 cut(s) 172, 409, 754
EcoRII CCWGG 1 cut(s) 247
EcoT14I CCWWGG 3 cut(s) 172, 409, 754
EcoT22I ATGCAT 1 cut(s) 25
ErhI CCWWGG 3 cut(s) 172, 409, 754
FaeI CATG 4 cut(s) 35, 413, 499, 758
FaqI GGGAC 2 cut(s) 195, 337
FatI CATG 4 cut(s) 31, 409, 495, 754
FbaI TGATCA 1 cut(s) 538
Fnu4HI GCNGC 4 cut(s) 51, 570, 635, 702
FokI GGATG 2 cut(s) 240, 409
Fsp4HI GCNGC 4 cut(s) 51, 570, 635, 702
FspBI CTAG 2 cut(s) 116, 492
GlaI GCGC 1 cut(s) 457
GluI GCNGC 4 cut(s) 51, 570, 635, 702
GsuI CTGGAG 1 cut(s) 402
HaeIII GGCC 1 cut(s) 408
HapII CCGG 2 cut(s) 290, 435
HgaI GACGC 1 cut(s) 709
HhaI GCGC 1 cut(s) 458
Hin1II CATG 4 cut(s) 35, 413, 499, 758
Hin6I GCGC 1 cut(s) 456
HinP1I GCGC 1 cut(s) 456
HincII GTYRAC 1 cut(s) 60
HindII GTYRAC 1 cut(s) 60
HindIII AAGCTT 1 cut(s) 341
HinfI GANTC 4 cut(s) 124, 233, 653, 759
HpaII CCGG 2 cut(s) 290, 435
HphI GGTGA 1 cut(s) 218
Hpy166II GTNNAC 2 cut(s) 60, 601
Hpy188I TCNGA 4 cut(s) 153, 365, 553, 683
Hpy188III TCNNGA 1 cut(s) 116
Hpy8I GTNNAC 2 cut(s) 60, 601
HpyAV CCTTC 3 cut(s) 203, 266, 678
HpyCH4III ACNGT 2 cut(s) 545, 768
HpyCH4IV ACGT 1 cut(s) 603
HpyCH4V TGCA 5 cut(s) 23, 50, 136, 333, 667
HpyF10VI GCNNNNNNNGC 4 cut(s) 506, 515, 664, 673
HpyF3I CTNAG 1 cut(s) 705
HpySE526I ACGT 1 cut(s) 603
Hsp92II CATG 4 cut(s) 35, 413, 499, 758
HspAI GCGC 1 cut(s) 456
KpnI GGTACC 1 cut(s) 417
Ksp22I TGATCA 1 cut(s) 538
Kzo9I GATC 2 cut(s) 385, 538
LmnI GCTCC 2 cut(s) 673, 772
Lsp1109I GCAGC 4 cut(s) 62, 556, 621, 688
MabI ACCWGGT 1 cut(s) 247
MaeI CTAG 2 cut(s) 116, 492
MaeII ACGT 1 cut(s) 603
MaeIII GTNAC 2 cut(s) 214, 557
MalI GATC 2 cut(s) 387, 540
MboI GATC 2 cut(s) 385, 538
MflI RGATCY 1 cut(s) 385
MhlI GDGCHC 1 cut(s) 609
MlsI TGGCCA 1 cut(s) 408
MluCI AATT 2 cut(s) 199, 677
MluI ACGCGT 1 cut(s) 462
MluNI TGGCCA 1 cut(s) 408
MmeI TCCRAC 3 cut(s) 450, 750, 787
MnlI CCTC 7 cut(s) 61, 268, 294, 297, 359, 431, 622
Mox20I TGGCCA 1 cut(s) 408
Mph1103I ATGCAT 1 cut(s) 25
MscI TGGCCA 1 cut(s) 408
Msp20I TGGCCA 1 cut(s) 408
MspA1I CMGCKG 2 cut(s) 634, 701
MspI CCGG 2 cut(s) 290, 435
MspR9I CCNGG 1 cut(s) 249
MvaI CCWGG 1 cut(s) 249
MvnI CGCG 1 cut(s) 464
MwoI GCNNNNNNNGC 4 cut(s) 506, 515, 664, 673
NcoI CCATGG 2 cut(s) 409, 754
NdeII GATC 2 cut(s) 385, 538
NlaIII CATG 4 cut(s) 35, 413, 499, 758
NlaIV GGNNCC 3 cut(s) 352, 415, 501
NmuCI GTSAC 1 cut(s) 557
NsiI ATGCAT 1 cut(s) 25
PfeI GAWTC 4 cut(s) 124, 233, 653, 759
PflMI CCANNNNNTGG 3 cut(s) 34, 178, 769
PkrI GCNGC 4 cut(s) 52, 571, 636, 703
Psp6I CCWGG 1 cut(s) 247
PspGI CCWGG 1 cut(s) 247
PspN4I GGNNCC 3 cut(s) 352, 415, 501
PsuI RGATCY 1 cut(s) 385
PvuII CAGCTG 2 cut(s) 634, 701
RsaI GTAC 2 cut(s) 415, 524
RsaNI GTAC 2 cut(s) 414, 523
SatI GCNGC 4 cut(s) 51, 570, 635, 702
Sau3AI GATC 2 cut(s) 385, 538
ScrFI CCNGG 1 cut(s) 249
SduI GDGCHC 1 cut(s) 609
SexAI ACCWGGT 1 cut(s) 247
Sse9I AATT 2 cut(s) 199, 677
SspMI CTAG 2 cut(s) 116, 492
StyD4I CCNGG 1 cut(s) 247
StyI CCWWGG 3 cut(s) 172, 409, 754
TaaI ACNGT 2 cut(s) 545, 768
TaiI ACGT 1 cut(s) 606
TasI AATT 2 cut(s) 199, 677
TfiI GAWTC 4 cut(s) 124, 233, 653, 759
TscAI CASTG 1 cut(s) 586
TseFI GTSAC 1 cut(s) 557
TseI GCWGC 4 cut(s) 50, 569, 634, 701
Tsp45I GTSAC 1 cut(s) 557
TspDTI ATGAA 4 cut(s) 17, 285, 725, 761
TspRI CASTG 1 cut(s) 586
Van91I CCANNNNNTGG 3 cut(s) 34, 178, 769
XbaI TCTAGA 1 cut(s) 115
XcmI CCANNNNNNNNNTGG 1 cut(s) 38
XspI CTAG 2 cut(s) 116, 492
Zsp2I ATGCAT 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.