Rroxscaffold_3G00256220

Subtilisin-like serine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000302
Physical Location & Seq
Forward (+)
50611012 .. 50613382
2371 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00256220.1

Sequence Viewer

Length: 1467 bp
ATGTCTAGCGTTCGAATCCCGTTTCATCCTGTGGCCAGCAGATTTGAGGGACCCTTTGGGTTTTTGCATCTGCGGTGCAGTGGATTAGTCTGGCTTTCTTTCGCCGCAATAACGGTGCAGGTCTGTACCGAGGCAGGTTGTGCCGAAAGTGACATGCTAGCAGCTCTTGACGCTGCTGTTGAGGACGGCGTGGATGTTCTCCCACTGTCCATAGGTGGTTCCTCAATTCCTTTCCATCAAGATGGGATTGCTATCGGCGCGTTTGGAGCAATTCAAAAGGGAATTTTTGTCAGCTGTTCAGCTGGAAACTCTGGCCCTTTCTATAGTTCCACAGTTGGAGCAAGCACCATTGACAGAAAAATAAGCTCATCAGCACAGCTTGGAGATTCTCCAAAATATTTTTACTCTGGTGAATCTTTATTCGATCCTAAAAATTTTAGCACTTCACCATTGCTACCTCTTGTTTATCCAGGTGCAAATCCCAATAACCAAACTGAGTTCTGTGCCTCGGGATCTCTTGTAAATGTTGAAGGGAAAGTAGTGGTGTGTGAGAGAGATGGAGGAGTGGGAACAATTGCCGAAGGAGAAGAAGTGAAGAGAGCAGGAGGTGCTGCCATGATTCTTGTGAACCAAATCACTGATGACTATAGCACCTTAGCTGATGCTCATGTACTTCCAGCAACAAATGTTGGTTTTCAAGCAGGAGATGCACTTGCTTCGATGGTTGCTTCTTTCTCATCCAGAGGACCGAGCATAGCCAGCCCCGGAATTTTGAAACCTGACATCATTGGTCCTGGTGTTAACACCCTCGCTGCATGGCCTGTTTCGGTGGATAATGTCACAACATTTAACATGATTTCAGGTACCTCAATGTCATGCCCGCACCTAAGTGGCATTGTAGCCTTGATCAAAAGTTCACACCCAGACTGGTCACCTGCTGCCATCAAATCAGCCATTATGACAACCGCTGATCTGCTAAACCTCAAAGGCACACCAATTGTTGATAGATCAACTACCCCAGTAGACATCTATAGCACTGGCTCAGGCCATGTCAACCCTTCAAAAGCCAATGACCCGGGGCTCATCTATGACACAAAACCAGAAGATTACATTCCTTACTTGTGTGGTCTGAACTCTGAACTACACAGACAATCAAATAGAGATAATCACCCAACAAAGAGGTCCTATCAAACTCAGAATTACACCAGAACTGTGACAAATGTTGGCCCGGCTAATTCAACCTACAAATTGGAGATTTTGGAACCCCAGAAATGGGGTGTGAGTGTGAATATGAGTGTGACTCCCTCAGTGCTTACATTCACACAGGTCAACCAGACCATTACATACAATGTGGAGTTTTCCACACAACTAGCTGGGCAAGATGGTTCATTTTCACAGGGATATTTGAGATGGGTTTCTGACAAGTATTCTGTATACAGCCCCATAGCTGTGATCTTTCAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

488

Amino Acids

51.62

Weight (kDa)

5.18

Isoelectric Point (pI)

34.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 38 - 325 1.2e-26 Subtilase family
PA PF02225 153 - 238 1.4e-09 PA domain
fn3_6 PF17766 395 - 484 7.8e-20 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 943
Acc36I ACCTGC 3 cut(s) 109, 125, 943
Acc65I GGTACC 1 cut(s) 863
AccB1I GGYRCC 1 cut(s) 863
AccI GTMKAC 2 cut(s) 1023, 1434
AccII CGCG 1 cut(s) 260
AciI CCGC 4 cut(s) 73, 105, 881, 966
AclWI GGATC 2 cut(s) 419, 520
AcoI YGGCCR 1 cut(s) 33
AcsI RAATTY 3 cut(s) 282, 433, 768
AfaI GTAC 3 cut(s) 127, 672, 865
AfiI CCNNNNNNNGG 2 cut(s) 1272, 1273
AgsI TTSAA 6 cut(s) 275, 530, 698, 775, 1062, 1239
AjnI CCWGG 2 cut(s) 469, 793
AleI CACNNNNGTG 1 cut(s) 888
AluBI AGCT 8 cut(s) 164, 294, 302, 366, 379, 659, 1373, 1448
AluI AGCT 8 cut(s) 164, 294, 302, 366, 379, 659, 1373, 1448
AlwI GGATC 2 cut(s) 419, 520
Ama87I CYCGRG 2 cut(s) 508, 1075
AoxI GGCC 5 cut(s) 33, 313, 818, 1045, 1225
ApeKI GCWGC 5 cut(s) 161, 173, 611, 812, 938
ApoI RAATTY 3 cut(s) 282, 433, 768
Asp718I GGTACC 1 cut(s) 863
AspLEI GCGC 1 cut(s) 260
AspS9I GGNCC 6 cut(s) 50, 314, 746, 791, 1182, 1226
AsuC2I CCSGG 4 cut(s) 765, 1076, 1077, 1229
AsuHPI GGTGA 4 cut(s) 422, 438, 924, 1160
AsuII TTCGAA 1 cut(s) 13
AsuNHI GCTAGC 1 cut(s) 157
AvaI CYCGRG 2 cut(s) 508, 1075
AvaII GGWCC 4 cut(s) 50, 746, 791, 1182
BalI TGGCCA 1 cut(s) 35
BanI GGYRCC 1 cut(s) 863
BanII GRGCYC 1 cut(s) 1083
BbvI GCAGC 5 cut(s) 160, 173, 598, 799, 925
BccI CCATC 7 cut(s) 236, 243, 551, 715, 950, 1376, 1404
BceAI ACGGC 1 cut(s) 202
BcgI CGANNNNNNTGC 2 cut(s) 699, 733
BciT130I CCWGG 2 cut(s) 471, 795
BclI TGATCA 1 cut(s) 906
BcnI CCSGG 4 cut(s) 765, 1076, 1077, 1229
BfaI CTAG 3 cut(s) 6, 158, 1370
BfmI CTRYAG 3 cut(s) 322, 646, 1030
BfuAI ACCTGC 3 cut(s) 109, 125, 943
BisI GCNGC 6 cut(s) 105, 162, 174, 612, 813, 939
BlsI GCNGC 6 cut(s) 106, 163, 175, 613, 814, 940
Bme1390I CCNGG 6 cut(s) 471, 765, 795, 1076, 1077, 1229
Bme18I GGWCC 4 cut(s) 50, 746, 791, 1182
BmeT110I CYCGRG 2 cut(s) 508, 1075
BmgT120I GGNCC 6 cut(s) 50, 314, 746, 791, 1182, 1226
BmiI GGNNCC 5 cut(s) 51, 52, 220, 865, 1263
BmrFI CCNGG 6 cut(s) 471, 765, 795, 1076, 1077, 1229
BmrI ACTGGG 1 cut(s) 1013
BmsI GCATC 3 cut(s) 76, 652, 697
BmtI GCTAGC 1 cut(s) 161
BmuI ACTGGG 1 cut(s) 1013
BplI GAGNNNNNCTC 2 cut(s) 1285, 1317
Bpu10I CCTNAGC 2 cut(s) 655, 1042
Bpu14I TTCGAA 1 cut(s) 13
BpuMI CCSGG 4 cut(s) 765, 1076, 1077, 1229
BsaBI GATNNNNATC 1 cut(s) 251
BsaJI CCNNGG 5 cut(s) 129, 507, 763, 1075, 1076
BsaXI ACNNNNNCTCC 2 cut(s) 576, 606
Bsc4I CCNNNNNNNGG 2 cut(s) 1272, 1273
Bse1I ACTGG 3 cut(s) 932, 1019, 1042
Bse3DI GCAATG 1 cut(s) 449
Bse8I GATNNNNATC 1 cut(s) 251
BseBI CCWGG 2 cut(s) 471, 795
BseDI CCNNGG 5 cut(s) 129, 507, 763, 1075, 1076
BseGI GGATG 3 cut(s) 25, 199, 737
BseJI GATNNNNATC 1 cut(s) 251
BseLI CCNNNNNNNGG 2 cut(s) 1272, 1273
BseMI GCAATG 1 cut(s) 449
BseMII CTCAG 4 cut(s) 486, 1056, 1208, 1320
BseNI ACTGG 3 cut(s) 932, 1019, 1042
BseRI GAGGAG 1 cut(s) 576
BseXI GCAGC 5 cut(s) 160, 173, 598, 799, 925
BseYI CCCAGC 1 cut(s) 1373
BsgI GTGCAG 2 cut(s) 97, 137
Bsh1236I CGCG 1 cut(s) 260
BshFI GGCC 5 cut(s) 35, 315, 820, 1047, 1227
BshNI GGYRCC 1 cut(s) 863
BsiHKCI CYCGRG 2 cut(s) 508, 1075
BsiSI CCGG 3 cut(s) 765, 1076, 1229
BslFI GGGAC 1 cut(s) 63
BslI CCNNNNNNNGG 2 cut(s) 1272, 1273
BsmFI GGGAC 1 cut(s) 63
BsnI GGCC 5 cut(s) 35, 315, 820, 1047, 1227
BsoBI CYCGRG 2 cut(s) 508, 1075
Bsp119I TTCGAA 1 cut(s) 13
Bsp1286I GDGCHC 1 cut(s) 1083
Bsp143I GATC 6 cut(s) 424, 512, 906, 970, 1007, 1452
BspACI CCGC 4 cut(s) 73, 105, 881, 966
BspANI GGCC 5 cut(s) 35, 315, 820, 1047, 1227
BspCNI CTCAG 4 cut(s) 487, 1055, 1207, 1319
BspFNI CGCG 1 cut(s) 260
BspLI GGNNCC 5 cut(s) 51, 52, 220, 865, 1263
BspMI ACCTGC 3 cut(s) 109, 125, 943
BspOI GCTAGC 1 cut(s) 161
BspPI GGATC 2 cut(s) 419, 520
BspT104I TTCGAA 1 cut(s) 13
BspT107I GGYRCC 1 cut(s) 863
BsrDI GCAATG 1 cut(s) 449
BsrI ACTGG 3 cut(s) 932, 1019, 1042
BssECI CCNNGG 5 cut(s) 129, 507, 763, 1075, 1076
BssMI GATC 6 cut(s) 424, 512, 906, 970, 1007, 1452
BssNAI GTATAC 1 cut(s) 1435
Bst1107I GTATAC 1 cut(s) 1435
Bst2UI CCWGG 2 cut(s) 471, 795
Bst4CI ACNGT 4 cut(s) 115, 207, 334, 1213
Bst6I CTCTTC 1 cut(s) 590
BstAPI GCANNNNNTGC 3 cut(s) 140, 608, 707
BstBI TTCGAA 1 cut(s) 13
BstC8I GCNNGC 5 cut(s) 37, 159, 343, 760, 881
BstDEI CTNAG 6 cut(s) 495, 655, 887, 1042, 1194, 1306
BstEII GGTNACC 1 cut(s) 930
BstF5I GGATG 3 cut(s) 25, 199, 737
BstFNI CGCG 1 cut(s) 260
BstHHI GCGC 1 cut(s) 260
BstKTI GATC 6 cut(s) 427, 515, 909, 973, 1010, 1455
BstMBI GATC 6 cut(s) 424, 512, 906, 970, 1007, 1452
BstMWI GCNNNNNNNGC 7 cut(s) 140, 170, 257, 266, 608, 707, 759
BstNI CCWGG 2 cut(s) 471, 795
BstNSI RCATGY 1 cut(s) 157
BstPI GGTNACC 1 cut(s) 930
BstSCI CCNGG 6 cut(s) 469, 763, 793, 1074, 1075, 1227
BstSFI CTRYAG 3 cut(s) 322, 646, 1030
BstUI CGCG 1 cut(s) 260
BstV1I GCAGC 5 cut(s) 160, 173, 598, 799, 925
BstX2I RGATCY 1 cut(s) 512
BstXI CCANNNNNNTGG 1 cut(s) 242
BstYI RGATCY 1 cut(s) 512
BstZ17I GTATAC 1 cut(s) 1435
BsuRI GGCC 5 cut(s) 35, 315, 820, 1047, 1227
BtsCI GGATG 3 cut(s) 25, 199, 737
BtsI GCAGTG 1 cut(s) 85
BtsIMutI CAGTG 5 cut(s) 85, 203, 636, 1035, 1314
BveI ACCTGC 3 cut(s) 109, 125, 943
Cac8I GCNNGC 5 cut(s) 37, 159, 343, 760, 881
CfoI GCGC 1 cut(s) 260
Cfr13I GGNCC 6 cut(s) 50, 314, 746, 791, 1182, 1226
Cfr9I CCCGGG 1 cut(s) 1075
CseI GACGC 1 cut(s) 179
Csp6I GTAC 3 cut(s) 126, 671, 864
CviAII CATG 7 cut(s) 154, 616, 668, 816, 853, 876, 1049
CviQI GTAC 3 cut(s) 126, 671, 864
DdeI CTNAG 6 cut(s) 495, 655, 887, 1042, 1194, 1306
DpnI GATC 6 cut(s) 426, 514, 908, 972, 1009, 1454
DpnII GATC 6 cut(s) 424, 512, 906, 970, 1007, 1452
EaeI YGGCCR 1 cut(s) 33
Eam1104I CTCTTC 1 cut(s) 590
EarI CTCTTC 1 cut(s) 590
Eco24I GRGCYC 1 cut(s) 1083
Eco47I GGWCC 4 cut(s) 50, 746, 791, 1182
Eco88I CYCGRG 2 cut(s) 508, 1075
Eco91I GGTNACC 1 cut(s) 930
EcoO109I RGGNCCY 2 cut(s) 50, 1182
EcoO65I GGTNACC 1 cut(s) 930
EcoRII CCWGG 2 cut(s) 469, 793
EcoT38I GRGCYC 1 cut(s) 1083
FaeI CATG 7 cut(s) 157, 619, 671, 819, 856, 879, 1052
FaqI GGGAC 1 cut(s) 63
FatI CATG 7 cut(s) 153, 615, 667, 815, 852, 875, 1048
FauI CCCGC 1 cut(s) 888
FbaI TGATCA 1 cut(s) 906
FblI GTMKAC 2 cut(s) 1023, 1434
Fnu4HI GCNGC 6 cut(s) 105, 162, 174, 612, 813, 939
FokI GGATG 3 cut(s) 12, 206, 724
FriOI GRGCYC 1 cut(s) 1083
Fsp4HI GCNGC 6 cut(s) 105, 162, 174, 612, 813, 939
FspBI CTAG 3 cut(s) 6, 158, 1370
GlaI GCGC 1 cut(s) 259
GluI GCNGC 6 cut(s) 105, 162, 174, 612, 813, 939
GsaI CCCAGC 1 cut(s) 1377
HaeIII GGCC 5 cut(s) 35, 315, 820, 1047, 1227
HapII CCGG 3 cut(s) 765, 1076, 1229
HgaI GACGC 1 cut(s) 179
HhaI GCGC 1 cut(s) 260
Hin1II CATG 7 cut(s) 157, 619, 671, 819, 856, 879, 1052
Hin6I GCGC 1 cut(s) 258
HinP1I GCGC 1 cut(s) 258
HincII GTYRAC 3 cut(s) 802, 1054, 1330
HindII GTYRAC 3 cut(s) 802, 1054, 1330
HinfI GANTC 5 cut(s) 15, 386, 413, 619, 1300
HpaI GTTAAC 1 cut(s) 802
HpaII CCGG 3 cut(s) 765, 1076, 1229
HphI GGTGA 4 cut(s) 422, 438, 924, 1160
Hpy166II GTNNAC 7 cut(s) 628, 802, 917, 1024, 1054, 1330, 1435
Hpy188I TCNGA 4 cut(s) 1131, 1138, 1197, 1420
Hpy188III TCNNGA 5 cut(s) 167, 239, 510, 741, 1464
Hpy8I GTNNAC 7 cut(s) 628, 802, 917, 1024, 1054, 1330, 1435
HpyAV CCTTC 3 cut(s) 524, 575, 1068
HpyCH4III ACNGT 4 cut(s) 115, 207, 334, 1213
HpyCH4V TGCA 6 cut(s) 67, 78, 118, 476, 710, 815
HpyF10VI GCNNNNNNNGC 7 cut(s) 140, 170, 257, 266, 608, 707, 759
HpyF3I CTNAG 6 cut(s) 495, 655, 887, 1042, 1194, 1306
Hsp92II CATG 7 cut(s) 157, 619, 671, 819, 856, 879, 1052
HspAI GCGC 1 cut(s) 258
KflI GGGWCCC 1 cut(s) 50
KpnI GGTACC 1 cut(s) 867
Ksp22I TGATCA 1 cut(s) 906
KspAI GTTAAC 1 cut(s) 802
Kzo9I GATC 6 cut(s) 424, 512, 906, 970, 1007, 1452
LmnI GCTCC 2 cut(s) 266, 338
Lsp1109I GCAGC 5 cut(s) 160, 173, 598, 799, 925
LweI GCATC 3 cut(s) 76, 652, 697
MaeI CTAG 3 cut(s) 6, 158, 1370
MaeIII GTNAC 5 cut(s) 149, 838, 930, 1213, 1297
MalI GATC 6 cut(s) 426, 514, 908, 972, 1009, 1454
MboI GATC 6 cut(s) 424, 512, 906, 970, 1007, 1452
MboII GAAGA 3 cut(s) 599, 607, 1115
MfeI CAATTG 2 cut(s) 573, 996
MflI RGATCY 1 cut(s) 512
MhlI GDGCHC 1 cut(s) 1083
MlsI TGGCCA 1 cut(s) 35
MluNI TGGCCA 1 cut(s) 35
MlyI GAGTC 1 cut(s) 1294
MmeI TCCRAC 1 cut(s) 316
Mox20I TGGCCA 1 cut(s) 35
MscI TGGCCA 1 cut(s) 35
MseI TTAA 2 cut(s) 801, 849
MslI CAYNNNNRTG 3 cut(s) 240, 888, 1448
Msp20I TGGCCA 1 cut(s) 35
MspA1I CMGCKG 3 cut(s) 294, 302, 968
MspI CCGG 3 cut(s) 765, 1076, 1229
MspR9I CCNGG 6 cut(s) 471, 765, 795, 1076, 1077, 1229
MunI CAATTG 2 cut(s) 573, 996
MvaI CCWGG 2 cut(s) 471, 795
MvnI CGCG 1 cut(s) 260
MwoI GCNNNNNNNGC 7 cut(s) 140, 170, 257, 266, 608, 707, 759
NciI CCSGG 4 cut(s) 765, 1076, 1077, 1229
NdeII GATC 6 cut(s) 424, 512, 906, 970, 1007, 1452
NheI GCTAGC 1 cut(s) 157
NlaIII CATG 7 cut(s) 157, 619, 671, 819, 856, 879, 1052
NlaIV GGNNCC 5 cut(s) 51, 52, 220, 865, 1263
NmuCI GTSAC 5 cut(s) 149, 838, 930, 1213, 1297
NspI RCATGY 1 cut(s) 157
NspV TTCGAA 1 cut(s) 13
OliI CACNNNNGTG 1 cut(s) 888
PaqCI CACCTGC 1 cut(s) 943
PfeI GAWTC 4 cut(s) 15, 386, 413, 619
PkrI GCNGC 6 cut(s) 106, 163, 175, 613, 814, 940
PleI GAGTC 1 cut(s) 1294
PpsI GAGTC 1 cut(s) 1294
PpuMI RGGWCCY 2 cut(s) 50, 1182
Psp5II RGGWCCY 2 cut(s) 50, 1182
Psp6I CCWGG 2 cut(s) 469, 793
PspEI GGTNACC 1 cut(s) 930
PspFI CCCAGC 1 cut(s) 1373
PspGI CCWGG 2 cut(s) 469, 793
PspN4I GGNNCC 5 cut(s) 51, 52, 220, 865, 1263
PspPI GGNCC 6 cut(s) 50, 314, 746, 791, 1182, 1226
PspPPI RGGWCCY 2 cut(s) 50, 1182
PsuI RGATCY 1 cut(s) 512
PvuII CAGCTG 2 cut(s) 294, 302
RsaI GTAC 3 cut(s) 127, 672, 865
RsaNI GTAC 3 cut(s) 126, 671, 864
RseI CAYNNNNRTG 3 cut(s) 240, 888, 1448
SaqAI TTAA 2 cut(s) 801, 849
SatI GCNGC 6 cut(s) 105, 162, 174, 612, 813, 939
Sau3AI GATC 6 cut(s) 424, 512, 906, 970, 1007, 1452
Sau96I GGNCC 6 cut(s) 50, 314, 746, 791, 1182, 1226
SchI GAGTC 1 cut(s) 1294
ScrFI CCNGG 6 cut(s) 471, 765, 795, 1076, 1077, 1229
SduI GDGCHC 1 cut(s) 1083
SfaNI GCATC 3 cut(s) 76, 652, 697
SfcI CTRYAG 3 cut(s) 322, 646, 1030
SfuI TTCGAA 1 cut(s) 13
SinI GGWCC 4 cut(s) 50, 746, 791, 1182
SmaI CCCGGG 1 cut(s) 1077
SmiMI CAYNNNNRTG 3 cut(s) 240, 888, 1448
SsiI CCGC 4 cut(s) 73, 105, 881, 966
SspI AATATT 1 cut(s) 398
SspMI CTAG 3 cut(s) 6, 158, 1370
StyD4I CCNGG 6 cut(s) 469, 763, 793, 1074, 1075, 1227
TaaI ACNGT 4 cut(s) 115, 207, 334, 1213
TaqI TCGA 3 cut(s) 13, 423, 719
TaqII GACCGA 1 cut(s) 763
TatI WGTACW 1 cut(s) 670
TauI GCSGC 1 cut(s) 107
TfiI GAWTC 4 cut(s) 15, 386, 413, 619
Tru1I TTAA 2 cut(s) 801, 849
Tru9I TTAA 2 cut(s) 801, 849
TscAI CASTG 5 cut(s) 85, 210, 643, 1042, 1314
TseFI GTSAC 5 cut(s) 149, 838, 930, 1213, 1297
TseI GCWGC 5 cut(s) 161, 173, 611, 812, 938
Tsp45I GTSAC 5 cut(s) 149, 838, 930, 1213, 1297
TspDTI ATGAA 2 cut(s) 14, 1377
TspMI CCCGGG 1 cut(s) 1075
TspRI CASTG 5 cut(s) 85, 210, 643, 1042, 1314
VpaK11BI GGWCC 4 cut(s) 50, 746, 791, 1182
XapI RAATTY 3 cut(s) 282, 433, 768
XceI RCATGY 1 cut(s) 157
XmaI CCCGGG 1 cut(s) 1075
XmiI GTMKAC 2 cut(s) 1023, 1434
XspI CTAG 3 cut(s) 6, 158, 1370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.