RchiOBHm_Chr7g0200021

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000302
Physical Location & Seq
Reverse (-)
17957581 .. 17958350
770 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17901

Sequence Viewer

Length: 639 bp
ATGTCATGTCCGCATCTCAGTGGAATTGCAGCTTTGCTCAAGAGCTCTCACCCATACTGGTCACCAGCTGCCATTAAATCTGCTATAATGACTTCTGCTGATCTTCTAAACTTGCAAGGCAAACGGATTTTTGATGAAACACTTCAGACTGCAGATATCTTAGCCACCGGTGCGGGCCATGTCAATCCATCAAAGGCAACCGACCCAGGACTGATATATGATATTCAACCTGATGATTATATTCCATATCTTTGTGGCTTGGGCTACAACGATACTGGAGTCAGTATCCTCGTGCACAGACCAATAAAGTGCTCAAAGGTATCAAGCATCCCTGAAGGAGAGCTGAACTACCCTTCATTTTCTGTCAAGCTGGGACCATCTCAGACATTCACAAGAACTGTGACAAATGTTGGTGCCTCATATTCCACTTATTCAGTCAAGGTATATGCACCACTAGGAACCCATGTGACTGTCAAACCCAGCACGCTTTACTTTACAGAGGTGAACCAGAAGGCGTCATATTCAGTGACATTCAGTCGTACTTCAGGAGGTAAAGCTGGTGCATTCACACAGGGATTTATAACATGGGCTTCTACTAATCACGTTGTCCGGAGTCCAGTTTCTGTTTTATTTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

212

Amino Acids

22.74

Weight (kDa)

8.91

Isoelectric Point (pI)

29.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 1 - 35 5.4e-07 Subtilase family
fn3_6 PF17766 114 - 209 1.3e-29 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 581
AccB1I GGYRCC 1 cut(s) 413
AccIII TCCGGA 1 cut(s) 609
AciI CCGC 2 cut(s) 11, 173
AcuI CTGAAG 3 cut(s) 128, 354, 528
AcyI GRCGYC 1 cut(s) 515
AfaI GTAC 1 cut(s) 541
AgeI ACCGGT 1 cut(s) 167
AgsI TTSAA 1 cut(s) 227
AhdI GACNNNNNGTC 1 cut(s) 534
AjnI CCWGG 1 cut(s) 205
AluBI AGCT 6 cut(s) 32, 45, 68, 343, 370, 557
AluI AGCT 6 cut(s) 32, 45, 68, 343, 370, 557
Alw21I GWGCWC 3 cut(s) 47, 297, 314
Alw44I GTGCAC 1 cut(s) 293
AlwNI CAGNNNCTG 1 cut(s) 623
Aor13HI TCCGGA 1 cut(s) 609
AoxI GGCC 1 cut(s) 175
ApaLI GTGCAC 1 cut(s) 293
ApeKI GCWGC 2 cut(s) 29, 68
AsiGI ACCGGT 1 cut(s) 167
Asp700I GAANNNNTTC 1 cut(s) 141
AspS9I GGNCC 2 cut(s) 175, 374
AsuHPI GGTGA 3 cut(s) 41, 54, 514
AvaII GGWCC 1 cut(s) 374
BaeGI GKGCMC 1 cut(s) 297
BaeI ACNNNNGTAYC 2 cut(s) 264, 297
BanI GGYRCC 1 cut(s) 413
BanII GRGCYC 1 cut(s) 47
BauI CACGAG 1 cut(s) 290
Bbv12I GWGCWC 3 cut(s) 47, 297, 314
BbvI GCAGC 2 cut(s) 41, 55
BccI CCATC 2 cut(s) 196, 385
BciT130I CCWGG 1 cut(s) 207
BciVI GTATCC 1 cut(s) 296
BfaI CTAG 1 cut(s) 455
BfmI CTRYAG 1 cut(s) 150
BfuI GTATCC 1 cut(s) 296
BisI GCNGC 2 cut(s) 30, 69
BlsI GCNGC 2 cut(s) 31, 70
Bme1390I CCNGG 1 cut(s) 207
Bme18I GGWCC 1 cut(s) 374
BmeRI GACNNNNNGTC 1 cut(s) 534
BmgT120I GGNCC 2 cut(s) 175, 374
BmiI GGNNCC 3 cut(s) 375, 415, 460
BmrFI CCNGG 1 cut(s) 207
BmsI GCATC 2 cut(s) 22, 336
BpmI CTGGAG 1 cut(s) 297
BpuEI CTTGAG 1 cut(s) 23
BsaHI GRCGYC 1 cut(s) 515
BsaJI CCNNGG 1 cut(s) 205
BsaWI WCCGGW 2 cut(s) 167, 609
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 3 cut(s) 62, 280, 617
BseAI TCCGGA 1 cut(s) 609
BseBI CCWGG 1 cut(s) 207
BseDI CCNNGG 1 cut(s) 205
BseGI GGATG 1 cut(s) 327
BseMII CTCAG 2 cut(s) 31, 395
BseNI ACTGG 3 cut(s) 62, 280, 617
BseSI GKGCMC 1 cut(s) 297
BseXI GCAGC 2 cut(s) 41, 55
BseYI CCCAGC 2 cut(s) 370, 479
BshFI GGCC 1 cut(s) 177
BshNI GGYRCC 1 cut(s) 413
BshTI ACCGGT 1 cut(s) 167
BsiHKAI GWGCWC 3 cut(s) 47, 297, 314
BsiSI CCGG 2 cut(s) 168, 610
BslFI GGGAC 1 cut(s) 387
BsmFI GGGAC 1 cut(s) 387
BsmI GAATGC 1 cut(s) 563
BsnI GGCC 1 cut(s) 177
Bsp1286I GDGCHC 3 cut(s) 47, 297, 314
Bsp13I TCCGGA 1 cut(s) 609
Bsp143I GATC 1 cut(s) 100
BspACI CCGC 2 cut(s) 11, 173
BspANI GGCC 1 cut(s) 177
BspCNI CTCAG 2 cut(s) 30, 394
BspEI TCCGGA 1 cut(s) 609
BspLI GGNNCC 3 cut(s) 375, 415, 460
BspMAI CTGCAG 1 cut(s) 154
BspT107I GGYRCC 1 cut(s) 413
BsrFI RCCGGY 1 cut(s) 167
BsrI ACTGG 3 cut(s) 62, 280, 617
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 1 cut(s) 205
BssMI GATC 1 cut(s) 100
BssNI GRCGYC 1 cut(s) 515
BssSI CACGAG 1 cut(s) 290
Bst2BI CACGAG 1 cut(s) 290
Bst2UI CCWGG 1 cut(s) 207
Bst4CI ACNGT 2 cut(s) 400, 472
BstACI GRCGYC 1 cut(s) 515
BstC8I GCNNGC 2 cut(s) 175, 485
BstDEI CTNAG 3 cut(s) 17, 160, 381
BstEII GGTNACC 1 cut(s) 60
BstF5I GGATG 1 cut(s) 327
BstKTI GATC 1 cut(s) 103
BstMBI GATC 1 cut(s) 100
BstMWI GCNNNNNNNGC 1 cut(s) 170
BstNI CCWGG 1 cut(s) 207
BstPI GGTNACC 1 cut(s) 60
BstSCI CCNGG 1 cut(s) 205
BstSFI CTRYAG 1 cut(s) 150
BstSLI GKGCMC 1 cut(s) 297
BstV1I GCAGC 2 cut(s) 41, 55
BsuI GTATCC 1 cut(s) 296
BsuRI GGCC 1 cut(s) 177
BtsCI GGATG 1 cut(s) 327
BtsIMutI CAGTG 2 cut(s) 25, 531
Cac8I GCNNGC 2 cut(s) 175, 485
CaiI CAGNNNCTG 1 cut(s) 623
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 2 cut(s) 175, 374
CseI GACGC 1 cut(s) 504
Csp6I GTAC 1 cut(s) 540
CspAI ACCGGT 1 cut(s) 167
CviAII CATG 4 cut(s) 6, 179, 464, 585
CviQI GTAC 1 cut(s) 540
DdeI CTNAG 3 cut(s) 17, 160, 381
DpnI GATC 1 cut(s) 102
DpnII GATC 1 cut(s) 100
DriI GACNNNNNGTC 1 cut(s) 534
Eam1105I GACNNNNNGTC 1 cut(s) 534
Ecl136II GAGCTC 1 cut(s) 45
Eco24I GRGCYC 1 cut(s) 47
Eco32I GATATC 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 374
Eco53kI GAGCTC 1 cut(s) 45
Eco57I CTGAAG 3 cut(s) 128, 354, 528
Eco91I GGTNACC 1 cut(s) 60
EcoICRI GAGCTC 1 cut(s) 45
EcoO65I GGTNACC 1 cut(s) 60
EcoRII CCWGG 1 cut(s) 205
EcoRV GATATC 1 cut(s) 157
EcoT38I GRGCYC 1 cut(s) 47
FaeI CATG 4 cut(s) 9, 182, 467, 588
FaqI GGGAC 1 cut(s) 387
FatI CATG 4 cut(s) 5, 178, 463, 584
FauI CCCGC 1 cut(s) 166
Fnu4HI GCNGC 2 cut(s) 30, 69
FokI GGATG 1 cut(s) 314
FriOI GRGCYC 1 cut(s) 47
Fsp4HI GCNGC 2 cut(s) 30, 69
FspBI CTAG 1 cut(s) 455
GluI GCNGC 2 cut(s) 30, 69
GsaI CCCAGC 2 cut(s) 374, 483
GsuI CTGGAG 1 cut(s) 297
HaeIII GGCC 1 cut(s) 177
HapII CCGG 2 cut(s) 168, 610
HgaI GACGC 1 cut(s) 504
Hin1I GRCGYC 1 cut(s) 515
Hin1II CATG 4 cut(s) 9, 182, 467, 588
HinfI GANTC 2 cut(s) 279, 613
HpaII CCGG 2 cut(s) 168, 610
HphI GGTGA 3 cut(s) 41, 54, 514
Hpy166II GTNNAC 2 cut(s) 295, 505
Hpy188I TCNGA 2 cut(s) 147, 384
Hpy188III TCNNGA 3 cut(s) 40, 546, 610
Hpy8I GTNNAC 2 cut(s) 295, 505
HpyAV CCTTC 3 cut(s) 329, 363, 505
HpyCH4III ACNGT 2 cut(s) 400, 472
HpyCH4IV ACGT 1 cut(s) 603
HpyCH4V TGCA 6 cut(s) 29, 115, 152, 295, 449, 563
HpyF10VI GCNNNNNNNGC 1 cut(s) 170
HpyF3I CTNAG 3 cut(s) 17, 160, 381
HpySE526I ACGT 1 cut(s) 603
Hsp92I GRCGYC 1 cut(s) 515
Hsp92II CATG 4 cut(s) 9, 182, 467, 588
Kpn2I TCCGGA 1 cut(s) 609
Kzo9I GATC 1 cut(s) 100
Lsp1109I GCAGC 2 cut(s) 41, 55
LweI GCATC 2 cut(s) 22, 336
MaeI CTAG 1 cut(s) 455
MaeII ACGT 1 cut(s) 603
MaeIII GTNAC 4 cut(s) 60, 400, 466, 526
MalI GATC 1 cut(s) 102
MboI GATC 1 cut(s) 100
MboII GAAGA 1 cut(s) 95
MhlI GDGCHC 3 cut(s) 47, 297, 314
MluCI AATT 1 cut(s) 24
MlyI GAGTC 2 cut(s) 288, 622
MnlI CCTC 4 cut(s) 299, 427, 493, 542
MroI TCCGGA 1 cut(s) 609
MroXI GAANNNNTTC 1 cut(s) 141
MseI TTAA 1 cut(s) 75
MslI CAYNNNNRTG 1 cut(s) 18
MspA1I CMGCKG 1 cut(s) 68
MspI CCGG 2 cut(s) 168, 610
MspR9I CCNGG 1 cut(s) 207
Mva1269I GAATGC 1 cut(s) 563
MvaI CCWGG 1 cut(s) 207
MwoI GCNNNNNNNGC 1 cut(s) 170
NdeII GATC 1 cut(s) 100
NlaIII CATG 4 cut(s) 9, 182, 467, 588
NlaIV GGNNCC 3 cut(s) 375, 415, 460
NmuCI GTSAC 4 cut(s) 60, 400, 466, 526
PctI GAATGC 1 cut(s) 563
PdmI GAANNNNTTC 1 cut(s) 141
PinAI ACCGGT 1 cut(s) 167
PkrI GCNGC 2 cut(s) 31, 70
PleI GAGTC 2 cut(s) 287, 621
PpsI GAGTC 2 cut(s) 287, 621
PsiI TTATAA 1 cut(s) 581
Psp124BI GAGCTC 1 cut(s) 47
Psp6I CCWGG 1 cut(s) 205
PspEI GGTNACC 1 cut(s) 60
PspFI CCCAGC 2 cut(s) 370, 479
PspGI CCWGG 1 cut(s) 205
PspN4I GGNNCC 3 cut(s) 375, 415, 460
PspPI GGNCC 2 cut(s) 175, 374
PstI CTGCAG 1 cut(s) 154
PstNI CAGNNNCTG 1 cut(s) 623
PvuII CAGCTG 1 cut(s) 68
RsaI GTAC 1 cut(s) 541
RsaNI GTAC 1 cut(s) 540
RseI CAYNNNNRTG 1 cut(s) 18
SacI GAGCTC 1 cut(s) 47
SaqAI TTAA 1 cut(s) 75
SatI GCNGC 2 cut(s) 30, 69
Sau3AI GATC 1 cut(s) 100
Sau96I GGNCC 2 cut(s) 175, 374
SchI GAGTC 2 cut(s) 288, 622
ScrFI CCNGG 1 cut(s) 207
SduI GDGCHC 3 cut(s) 47, 297, 314
SfaNI GCATC 2 cut(s) 22, 336
SfcI CTRYAG 1 cut(s) 150
SgrAI CRCCGGYG 1 cut(s) 167
SinI GGWCC 1 cut(s) 374
SmiMI CAYNNNNRTG 1 cut(s) 18
SmlI CTYRAG 1 cut(s) 38
SmoI CTYRAG 1 cut(s) 38
Sse9I AATT 1 cut(s) 24
SsiI CCGC 2 cut(s) 11, 173
SspMI CTAG 1 cut(s) 455
SstI GAGCTC 1 cut(s) 47
StyD4I CCNGG 1 cut(s) 205
TaaI ACNGT 2 cut(s) 400, 472
TaiI ACGT 1 cut(s) 606
TasI AATT 1 cut(s) 24
Tru1I TTAA 1 cut(s) 75
Tru9I TTAA 1 cut(s) 75
TscAI CASTG 2 cut(s) 25, 531
TseFI GTSAC 4 cut(s) 60, 400, 466, 526
TseI GCWGC 2 cut(s) 29, 68
Tsp45I GTSAC 4 cut(s) 60, 400, 466, 526
TspDTI ATGAA 2 cut(s) 150, 345
TspGWI ACGGA 1 cut(s) 139
TspRI CASTG 2 cut(s) 25, 531
VneI GTGCAC 1 cut(s) 293
VpaK11BI GGWCC 1 cut(s) 374
XmnI GAANNNNTTC 1 cut(s) 141
XspI CTAG 1 cut(s) 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.