Rh7CG193600

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0000302
Physical Location & Seq
Reverse (-)
17052808 .. 17055263
2456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG193600.1

Sequence Viewer

Length: 1272 bp
ATGTTGCAACAAAAGCCGACTTTCATGACCTCGAATCACCATTGGCTGCATAAGAAATGCCGGTACGTGAGCTTTCAGATTCTTAAAGTCCTCCACAATCTCTTGCTATCTATATATAGGAGCTTGAATGGTGACCATAGCCATGATTCATCAGCTCTTTGCGATGTAGGGTCCCTTACAAATGTTGAAGGGAAAGTAGTGGTCTGTGAGCTAGGTGGCGGAATTGGAAGAATTGCAAAAGGGGTAGAAGTGAAAAGAGCTGGTGGTGTTGCCATGATTCTTGTCAACCCAGACTTTGCTGGCTATACCACCATAGCAGACGCTCACGTGCTTCCGGCAACACATGTGAGTTACTCTGCGGGGGTGAGTATCAAAACCTATATAAACTCAACCTCAACTCCTACAGCAACAATCTTGTTCAAAGGCACTGTCATTGGTGATCAGCTTGCTCCGAAGGTCTCTTTCTTTTCATCAAGAGGACCAAGCCTCGCAAGTCCTGGCATTTTGAAACCTGACATAATTGGTCCCGGTGTGAGCATCCTAGCTGCATGGCCTTTTTCAGTGGACAATGCCACAGAATCTAAAGCCACATTTAACATTATTTCCGGTACTTCAATGTCATGCCCTCACCTGAGTGGCATTGTAGCCTTACTCAAGAGCTCACACCCTGACTGGTCACCAGCTGCCATTAAGTCTGCCATCATGACAACTGCTGAAGTAAACAACCTCGCAGGCTCTGCCATAATGGATGAAACACTATTTGCAGCAGACCTCTTTGCCATTGGTGCAGGTCATGTTAACCCTTCAAAAGCAAATGACCCTGGGCTCATCTACGACACGCAACCAGATGACTACATTCCTTACATGTGTGGTTTGAACTACACAGACCAACAGATAGCAATAGTCACCCGGAAATTAGTGAAATGCTCTGAAGTAGGAGCCATACCTGAAGCACAGCTAAATTATCCCACATTTTCTCTCTTTATAGATCCTGCTGAGAAGCCTCAGAATTACACAAGAACAGTGACGAATGTTGGCCCCGCTAAATCAACTTATGAATTAGCCGTTGTAAGTCCACATAGCATAGACATCAGTGTGCAACCTGAGGAGCTCACATTCACAGAGGTTAACCAGACAATGACGTATCATGTGGTGTTTACCGCACAAAAAGGTGCTGTGAAGGATGGTATAAAATTTTCTCAGGGATATCTGAGCCGGGTCTCTCGCCAGCATTCTGTTAGAAGCCCAATATCTGTGATCTTTGACACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

423

Amino Acids

45.39

Weight (kDa)

6.91

Isoelectric Point (pI)

29.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PA PF02225 54 - 123 4.7e-10 PA domain
Peptidase_S8 PF00082 148 - 241 6.2e-18 Subtilase family
fn3_6 PF17766 319 - 419 2.3e-25 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 779
AciI CCGC 4 cut(s) 219, 359, 1041, 1161
AclWI GGATC 1 cut(s) 983
AcsI RAATTY 1 cut(s) 1193
AcuI CTGAAG 3 cut(s) 735, 951, 969
AcvI CACGTG 1 cut(s) 328
AfaI GTAC 2 cut(s) 65, 610
AflIII ACRYGT 2 cut(s) 343, 864
AgsI TTSAA 7 cut(s) 127, 188, 421, 508, 615, 807, 877
AjnI CCWGG 2 cut(s) 496, 820
AleI CACNNNNGTG 1 cut(s) 633
Alw21I GWGCWC 2 cut(s) 662, 1113
Alw26I GTCTC 2 cut(s) 463, 1225
AlwI GGATC 1 cut(s) 983
AlwNI CAGNNNCTG 1 cut(s) 737
AoxI GGCC 2 cut(s) 551, 1036
ApeKI GCWGC 4 cut(s) 46, 545, 683, 764
ApoI RAATTY 1 cut(s) 1193
ArsI GACNNNNNNTTYG 2 cut(s) 414, 446
AspS9I GGNCC 4 cut(s) 171, 479, 524, 1037
AsuC2I CCSGG 3 cut(s) 528, 910, 1217
AsuHPI GGTGA 7 cut(s) 29, 143, 376, 449, 620, 669, 898
AvaII GGWCC 3 cut(s) 171, 479, 524
AxyI CCTNAGG 1 cut(s) 1104
BanII GRGCYC 3 cut(s) 662, 828, 1113
BbrPI CACGTG 1 cut(s) 328
Bbv12I GWGCWC 2 cut(s) 662, 1113
BbvI GCAGC 4 cut(s) 33, 532, 670, 776
BccI CCATC 2 cut(s) 707, 1178
BceAI ACGGC 1 cut(s) 1049
BciT130I CCWGG 2 cut(s) 498, 822
BclI TGATCA 1 cut(s) 439
BcnI CCSGG 3 cut(s) 528, 910, 1217
BcoDI GTCTC 2 cut(s) 463, 1225
BfaI CTAG 2 cut(s) 212, 542
BfmI CTRYAG 1 cut(s) 402
BfuAI ACCTGC 1 cut(s) 779
BisI GCNGC 4 cut(s) 47, 546, 684, 765
BlsI GCNGC 4 cut(s) 48, 547, 685, 766
Bme1390I CCNGG 5 cut(s) 498, 528, 822, 910, 1217
Bme18I GGWCC 3 cut(s) 171, 479, 524
BmgT120I GGNCC 4 cut(s) 171, 479, 524, 1037
BmiI GGNNCC 5 cut(s) 172, 173, 526, 940, 1039
BmrFI CCNGG 5 cut(s) 498, 528, 822, 910, 1217
BmsI GCATC 1 cut(s) 546
BpuEI CTTGAG 1 cut(s) 638
BpuMI CCSGG 3 cut(s) 528, 910, 1217
BsaAI YACGTR 2 cut(s) 67, 328
BsaI GGTCTC 2 cut(s) 463, 1225
BsaJI CCNNGG 2 cut(s) 820, 821
BsaWI WCCGGW 1 cut(s) 605
BsaXI ACNNNNNCTCC 2 cut(s) 382, 412
Bse118I RCCGGY 1 cut(s) 60
Bse1I ACTGG 1 cut(s) 677
Bse21I CCTNAGG 1 cut(s) 1104
BseBI CCWGG 2 cut(s) 498, 822
BseDI CCNNGG 2 cut(s) 820, 821
BseGI GGATG 3 cut(s) 537, 754, 1189
BseMII CTCAG 6 cut(s) 623, 987, 1019, 1095, 1202, 1214
BseNI ACTGG 1 cut(s) 677
BseRI GAGGAG 1 cut(s) 1121
BseXI GCAGC 4 cut(s) 33, 532, 670, 776
BsgI GTGCAG 1 cut(s) 807
BshFI GGCC 2 cut(s) 553, 1038
BsiHKAI GWGCWC 2 cut(s) 662, 1113
BsiSI CCGG 6 cut(s) 61, 335, 528, 606, 910, 1216
BslFI GGGAC 2 cut(s) 157, 510
BsmAI GTCTC 2 cut(s) 463, 1225
BsmFI GGGAC 2 cut(s) 157, 510
BsmI GAATGC 1 cut(s) 1231
BsnI GGCC 2 cut(s) 553, 1038
Bso31I GGTCTC 2 cut(s) 463, 1225
Bsp1286I GDGCHC 3 cut(s) 662, 828, 1113
Bsp143I GATC 3 cut(s) 439, 988, 1257
BspACI CCGC 4 cut(s) 219, 359, 1041, 1161
BspANI GGCC 2 cut(s) 553, 1038
BspCNI CTCAG 6 cut(s) 624, 988, 1018, 1096, 1203, 1213
BspHI TCATGA 2 cut(s) 24, 702
BspLI GGNNCC 5 cut(s) 172, 173, 526, 940, 1039
BspMI ACCTGC 1 cut(s) 779
BspPI GGATC 1 cut(s) 983
BspTNI GGTCTC 2 cut(s) 463, 1225
BsrFI RCCGGY 1 cut(s) 60
BsrI ACTGG 1 cut(s) 677
BssAI RCCGGY 1 cut(s) 60
BssECI CCNNGG 2 cut(s) 820, 821
BssMI GATC 3 cut(s) 439, 988, 1257
Bst2UI CCWGG 2 cut(s) 498, 822
Bst4CI ACNGT 2 cut(s) 430, 1024
BstAPI GCANNNNNTGC 1 cut(s) 737
BstBAI YACGTR 2 cut(s) 67, 328
BstC8I GCNNGC 4 cut(s) 301, 447, 733, 1229
BstDEI CTNAG 6 cut(s) 632, 996, 1005, 1104, 1200, 1211
BstEII GGTNACC 2 cut(s) 131, 675
BstF5I GGATG 3 cut(s) 537, 754, 1189
BstKTI GATC 3 cut(s) 442, 991, 1260
BstMAI GTCTC 2 cut(s) 463, 1225
BstMBI GATC 3 cut(s) 439, 988, 1257
BstMWI GCNNNNNNNGC 3 cut(s) 13, 737, 785
BstNI CCWGG 2 cut(s) 498, 822
BstNSI RCATGY 2 cut(s) 347, 868
BstPI GGTNACC 2 cut(s) 131, 675
BstSCI CCNGG 5 cut(s) 496, 526, 820, 908, 1215
BstSFI CTRYAG 1 cut(s) 402
BstV1I GCAGC 4 cut(s) 33, 532, 670, 776
BstX2I RGATCY 1 cut(s) 988
BstYI RGATCY 1 cut(s) 988
Bsu36I CCTNAGG 1 cut(s) 1104
BsuRI GGCC 2 cut(s) 553, 1038
BtgZI GCGATG 1 cut(s) 177
BtsCI GGATG 3 cut(s) 537, 754, 1189
BtsIMutI CAGTG 4 cut(s) 426, 567, 1029, 1099
BveI ACCTGC 1 cut(s) 779
Cac8I GCNNGC 4 cut(s) 301, 447, 733, 1229
CaiI CAGNNNCTG 1 cut(s) 737
CciI TCATGA 2 cut(s) 24, 702
Cfr10I RCCGGY 1 cut(s) 60
Cfr13I GGNCC 4 cut(s) 171, 479, 524, 1037
CseI GACGC 1 cut(s) 329
Csp6I GTAC 2 cut(s) 64, 609
CviQI GTAC 2 cut(s) 64, 609
DdeI CTNAG 6 cut(s) 632, 996, 1005, 1104, 1200, 1211
DpnI GATC 3 cut(s) 441, 990, 1259
DpnII GATC 3 cut(s) 439, 988, 1257
EciI GGCGGA 1 cut(s) 234
Ecl136II GAGCTC 2 cut(s) 660, 1111
Eco24I GRGCYC 3 cut(s) 662, 828, 1113
Eco31I GGTCTC 2 cut(s) 463, 1225
Eco32I GATATC 1 cut(s) 1208
Eco47I GGWCC 3 cut(s) 171, 479, 524
Eco53kI GAGCTC 2 cut(s) 660, 1111
Eco57I CTGAAG 3 cut(s) 735, 951, 969
Eco72I CACGTG 1 cut(s) 328
Eco81I CCTNAGG 1 cut(s) 1104
Eco91I GGTNACC 2 cut(s) 131, 675
EcoICRI GAGCTC 2 cut(s) 660, 1111
EcoO109I RGGNCCY 1 cut(s) 171
EcoO65I GGTNACC 2 cut(s) 131, 675
EcoRII CCWGG 2 cut(s) 496, 820
EcoRV GATATC 1 cut(s) 1208
EcoT38I GRGCYC 3 cut(s) 662, 828, 1113
FaqI GGGAC 2 cut(s) 157, 510
FauI CCCGC 2 cut(s) 352, 1048
FbaI TGATCA 1 cut(s) 439
Fnu4HI GCNGC 4 cut(s) 47, 546, 684, 765
FokI GGATG 3 cut(s) 524, 761, 1196
FriOI GRGCYC 3 cut(s) 662, 828, 1113
Fsp4HI GCNGC 4 cut(s) 47, 546, 684, 765
FspBI CTAG 2 cut(s) 212, 542
GluI GCNGC 4 cut(s) 47, 546, 684, 765
HaeIII GGCC 2 cut(s) 553, 1038
HapII CCGG 6 cut(s) 61, 335, 528, 606, 910, 1216
HgaI GACGC 1 cut(s) 329
HincII GTYRAC 3 cut(s) 286, 799, 1129
HindII GTYRAC 3 cut(s) 286, 799, 1129
HinfI GANTC 5 cut(s) 34, 79, 146, 277, 578
HpaI GTTAAC 2 cut(s) 799, 1129
HpaII CCGG 6 cut(s) 61, 335, 528, 606, 910, 1216
HphI GGTGA 7 cut(s) 29, 143, 376, 449, 620, 669, 898
Hpy166II GTNNAC 7 cut(s) 286, 565, 721, 799, 1076, 1129, 1158
Hpy188I TCNGA 5 cut(s) 78, 453, 931, 1008, 1212
Hpy188III TCNNGA 4 cut(s) 25, 474, 655, 703
Hpy8I GTNNAC 7 cut(s) 286, 565, 721, 799, 1076, 1129, 1158
HpyAV CCTTC 4 cut(s) 182, 448, 813, 1174
HpyCH4III ACNGT 2 cut(s) 430, 1024
HpyCH4IV ACGT 3 cut(s) 66, 327, 1142
HpyCH4V TGCA 7 cut(s) 7, 49, 236, 548, 764, 788, 1099
HpyF10VI GCNNNNNNNGC 3 cut(s) 13, 737, 785
HpyF3I CTNAG 6 cut(s) 632, 996, 1005, 1104, 1200, 1211
HpySE526I ACGT 3 cut(s) 66, 327, 1142
KflI GGGWCCC 1 cut(s) 171
Ksp22I TGATCA 1 cut(s) 439
KspAI GTTAAC 2 cut(s) 799, 1129
Kzo9I GATC 3 cut(s) 439, 988, 1257
LmnI GCTCC 4 cut(s) 120, 454, 938, 1108
Lsp1109I GCAGC 4 cut(s) 33, 532, 670, 776
LweI GCATC 1 cut(s) 546
MaeI CTAG 2 cut(s) 212, 542
MaeII ACGT 3 cut(s) 66, 327, 1142
MaeIII GTNAC 5 cut(s) 131, 350, 675, 904, 1024
MalI GATC 3 cut(s) 441, 990, 1259
MboI GATC 3 cut(s) 439, 988, 1257
MboII GAAGA 1 cut(s) 240
MflI RGATCY 1 cut(s) 988
MhlI GDGCHC 3 cut(s) 662, 828, 1113
MluCI AATT 8 cut(s) 222, 231, 519, 914, 961, 1009, 1058, 1193
MseI TTAA 6 cut(s) 84, 594, 690, 798, 1128, 1270
MslI CAYNNNNRTG 3 cut(s) 141, 633, 1094
MspA1I CMGCKG 1 cut(s) 683
MspI CCGG 6 cut(s) 61, 335, 528, 606, 910, 1216
MspR9I CCNGG 5 cut(s) 498, 528, 822, 910, 1217
Mva1269I GAATGC 1 cut(s) 1231
MvaI CCWGG 2 cut(s) 498, 822
MwoI GCNNNNNNNGC 3 cut(s) 13, 737, 785
NciI CCSGG 3 cut(s) 528, 910, 1217
NdeII GATC 3 cut(s) 439, 988, 1257
NlaIV GGNNCC 5 cut(s) 172, 173, 526, 940, 1039
NmuCI GTSAC 4 cut(s) 131, 675, 904, 1024
NspI RCATGY 2 cut(s) 347, 868
OliI CACNNNNGTG 1 cut(s) 633
PagI TCATGA 2 cut(s) 24, 702
PasI CCCWGGG 1 cut(s) 821
PciI ACATGT 2 cut(s) 343, 864
PctI GAATGC 1 cut(s) 1231
PfeI GAWTC 5 cut(s) 34, 79, 146, 277, 578
PkrI GCNGC 4 cut(s) 48, 547, 685, 766
PmaCI CACGTG 1 cut(s) 328
PmlI CACGTG 1 cut(s) 328
Ppu21I YACGTR 2 cut(s) 67, 328
PpuMI RGGWCCY 1 cut(s) 171
PscI ACATGT 2 cut(s) 343, 864
Psp124BI GAGCTC 2 cut(s) 662, 1113
Psp5II RGGWCCY 1 cut(s) 171
Psp6I CCWGG 2 cut(s) 496, 820
PspCI CACGTG 1 cut(s) 328
PspEI GGTNACC 2 cut(s) 131, 675
PspGI CCWGG 2 cut(s) 496, 820
PspN4I GGNNCC 5 cut(s) 172, 173, 526, 940, 1039
PspPI GGNCC 4 cut(s) 171, 479, 524, 1037
PspPPI RGGWCCY 1 cut(s) 171
PstNI CAGNNNCTG 1 cut(s) 737
PsuI RGATCY 1 cut(s) 988
PvuII CAGCTG 1 cut(s) 683
RsaI GTAC 2 cut(s) 65, 610
RsaNI GTAC 2 cut(s) 64, 609
RseI CAYNNNNRTG 3 cut(s) 141, 633, 1094
SacI GAGCTC 2 cut(s) 662, 1113
SaqAI TTAA 6 cut(s) 84, 594, 690, 798, 1128, 1270
SatI GCNGC 4 cut(s) 47, 546, 684, 765
Sau3AI GATC 3 cut(s) 439, 988, 1257
Sau96I GGNCC 4 cut(s) 171, 479, 524, 1037
ScrFI CCNGG 5 cut(s) 498, 528, 822, 910, 1217
SduI GDGCHC 3 cut(s) 662, 828, 1113
SfaNI GCATC 1 cut(s) 546
SfcI CTRYAG 1 cut(s) 402
SinI GGWCC 3 cut(s) 171, 479, 524
SmiMI CAYNNNNRTG 3 cut(s) 141, 633, 1094
SmlI CTYRAG 1 cut(s) 653
SmoI CTYRAG 1 cut(s) 653
Sse9I AATT 8 cut(s) 222, 231, 519, 914, 961, 1009, 1058, 1193
SsiI CCGC 4 cut(s) 219, 359, 1041, 1161
SspMI CTAG 2 cut(s) 212, 542
SstI GAGCTC 2 cut(s) 662, 1113
StyD4I CCNGG 5 cut(s) 496, 526, 820, 908, 1215
TaaI ACNGT 2 cut(s) 430, 1024
TaiI ACGT 3 cut(s) 69, 330, 1145
TaqI TCGA 1 cut(s) 32
TasI AATT 8 cut(s) 222, 231, 519, 914, 961, 1009, 1058, 1193
TfiI GAWTC 5 cut(s) 34, 79, 146, 277, 578
Tru1I TTAA 6 cut(s) 84, 594, 690, 798, 1128, 1270
Tru9I TTAA 6 cut(s) 84, 594, 690, 798, 1128, 1270
TscAI CASTG 4 cut(s) 433, 567, 1029, 1099
TseFI GTSAC 4 cut(s) 131, 675, 904, 1024
TseI GCWGC 4 cut(s) 46, 545, 683, 764
Tsp45I GTSAC 4 cut(s) 131, 675, 904, 1024
TspDTI ATGAA 5 cut(s) 13, 138, 459, 765, 1071
TspRI CASTG 4 cut(s) 433, 567, 1029, 1099
VpaK11BI GGWCC 3 cut(s) 171, 479, 524
XapI RAATTY 1 cut(s) 1193
XceI RCATGY 2 cut(s) 347, 868
XspI CTAG 2 cut(s) 212, 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.