Rh7CG193800

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0000302
Physical Location & Seq
Reverse (-)
17058646 .. 17059176
531 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG193800.1

Sequence Viewer

Length: 531 bp
ATGGTTTATGCATACCGCAATGTGGCCACTGGATTTGCAGCAAAGCTGACCGCGGAGGAAGTTGAAGAAATGGAGAAGAAAGATGGGTTTATCTCGGCTCATCCAGAACGGAATCTGCAATTGCACATAACTCATAGTCTTAACTTCTTGGGGCTGCAACAAGGATTGGGACTTTGGAAAGGGTCAAATAATGGTGAAGGAGTGATTATTGGACTTTTGAATACTGGGATATTCCCAGACCATCCTTCATTTAGTGATGAAGGAGTACCACCTCCTCCAGCTAAATGGAAAGGCAAGTGTGATTTCCGCGGGACAGTCTGCAATAACAAGCTTATTGGCACCAGAAGTTTTGATGGTGGAAAGACCACAGGAGGCCCTCCAATTGACGATGCAGGCCATGGAACTCATACTTCAAGCACAGCTGGAGGAAACTTTGTGAAAGGTGCTGGAGTGTTTGGAATGGCCAACGTCACAGCAACTAGCATGGCACCTCATGCTCACTTGGCAATGTACAAAGTCTGCTCTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

176

Amino Acids

18.58

Weight (kDa)

6.7

Isoelectric Point (pI)

31.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 1 - 42 7e-07 Peptidase inhibitor I9
Peptidase_S8 PF00082 65 - 174 1.6e-07 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 338, 487
AccII CGCG 2 cut(s) 53, 309
AciI CCGC 5 cut(s) 16, 51, 53, 307, 309
AcoI YGGCCR 2 cut(s) 24, 462
AfaI GTAC 2 cut(s) 267, 512
AfiI CCNNNNNNNGG 1 cut(s) 22
AgsI TTSAA 3 cut(s) 65, 220, 414
AluBI AGCT 4 cut(s) 46, 281, 331, 422
AluI AGCT 4 cut(s) 46, 281, 331, 422
AoxI GGCC 4 cut(s) 24, 373, 394, 462
ApeKI GCWGC 2 cut(s) 38, 154
AspS9I GGNCC 1 cut(s) 374
AsuHPI GGTGA 1 cut(s) 206
BalI TGGCCA 2 cut(s) 26, 464
BanI GGYRCC 2 cut(s) 338, 487
BbvI GCAGC 2 cut(s) 50, 141
BccI CCATC 3 cut(s) 77, 249, 347
BfaI CTAG 1 cut(s) 480
BisI GCNGC 2 cut(s) 39, 155
BlsI GCNGC 2 cut(s) 40, 156
BmgT120I GGNCC 1 cut(s) 374
BmiI GGNNCC 2 cut(s) 340, 489
BmrI ACTGGG 1 cut(s) 234
BmsI GCATC 1 cut(s) 379
BmuI ACTGGG 1 cut(s) 234
BpmI CTGGAG 3 cut(s) 261, 444, 468
BsaJI CCNNGG 3 cut(s) 51, 307, 397
Bsc4I CCNNNNNNNGG 1 cut(s) 22
Bse1I ACTGG 2 cut(s) 34, 229
Bse3DI GCAATG 2 cut(s) 25, 513
BseDI CCNNGG 3 cut(s) 51, 307, 397
BseGI GGATG 2 cut(s) 100, 241
BseLI CCNNNNNNNGG 1 cut(s) 22
BseMI GCAATG 2 cut(s) 25, 513
BseNI ACTGG 2 cut(s) 34, 229
BseRI GAGGAG 1 cut(s) 264
BseXI GCAGC 2 cut(s) 50, 141
Bsh1236I CGCG 2 cut(s) 53, 309
BshFI GGCC 4 cut(s) 26, 375, 396, 464
BshNI GGYRCC 2 cut(s) 338, 487
BslFI GGGAC 2 cut(s) 183, 325
BslI CCNNNNNNNGG 1 cut(s) 22
BsmFI GGGAC 2 cut(s) 183, 325
BsnI GGCC 4 cut(s) 26, 375, 396, 464
Bsp1407I TGTACA 1 cut(s) 510
Bsp19I CCATGG 1 cut(s) 397
BspACI CCGC 5 cut(s) 16, 51, 53, 307, 309
BspANI GGCC 4 cut(s) 26, 375, 396, 464
BspFNI CGCG 2 cut(s) 53, 309
BspLI GGNNCC 2 cut(s) 340, 489
BspT107I GGYRCC 2 cut(s) 338, 487
BsrDI GCAATG 2 cut(s) 25, 513
BsrGI TGTACA 1 cut(s) 510
BsrI ACTGG 2 cut(s) 34, 229
BssECI CCNNGG 3 cut(s) 51, 307, 397
BssT1I CCWWGG 1 cut(s) 397
Bst4CI ACNGT 1 cut(s) 316
BstAPI GCANNNNNTGC 1 cut(s) 494
BstAUI TGTACA 1 cut(s) 510
BstC8I GCNNGC 1 cut(s) 394
BstDSI CCRYGG 3 cut(s) 51, 307, 397
BstF5I GGATG 2 cut(s) 100, 241
BstFNI CGCG 2 cut(s) 53, 309
BstMWI GCNNNNNNNGC 2 cut(s) 494, 503
BstUI CGCG 2 cut(s) 53, 309
BstV1I GCAGC 2 cut(s) 50, 141
BstXI CCANNNNNNTGG 1 cut(s) 285
BsuRI GGCC 4 cut(s) 26, 375, 396, 464
BtgI CCRYGG 3 cut(s) 51, 307, 397
BtsCI GGATG 2 cut(s) 100, 241
BtsIMutI CAGTG 1 cut(s) 27
Cac8I GCNNGC 1 cut(s) 394
Cfr13I GGNCC 1 cut(s) 374
Cfr42I CCGCGG 2 cut(s) 54, 310
Csp6I GTAC 2 cut(s) 266, 511
CspCI CAANNNNNGTGG 2 cut(s) 16, 51
CviAII CATG 3 cut(s) 398, 484, 494
CviQI GTAC 2 cut(s) 266, 511
EaeI YGGCCR 2 cut(s) 24, 462
Eco130I CCWWGG 1 cut(s) 397
EcoO109I RGGNCCY 1 cut(s) 374
EcoT14I CCWWGG 1 cut(s) 397
EcoT22I ATGCAT 1 cut(s) 13
ErhI CCWWGG 1 cut(s) 397
FaeI CATG 3 cut(s) 401, 487, 497
FaiI YATR 8 cut(s) 9, 13, 128, 135, 399, 408, 485, 495
FaqI GGGAC 2 cut(s) 183, 325
FatI CATG 3 cut(s) 397, 483, 493
FauI CCCGC 1 cut(s) 302
Fnu4HI GCNGC 2 cut(s) 39, 155
FokI GGATG 2 cut(s) 87, 228
Fsp4HI GCNGC 2 cut(s) 39, 155
FspBI CTAG 1 cut(s) 480
GluI GCNGC 2 cut(s) 39, 155
GsuI CTGGAG 3 cut(s) 261, 444, 468
HaeIII GGCC 4 cut(s) 26, 375, 396, 464
Hin1II CATG 3 cut(s) 401, 487, 497
HindIII AAGCTT 1 cut(s) 329
HinfI GANTC 1 cut(s) 112
HphI GGTGA 1 cut(s) 206
Hpy188I TCNGA 1 cut(s) 526
Hpy188III TCNNGA 1 cut(s) 104
HpyAV CCTTC 3 cut(s) 191, 254, 255
HpyCH4III ACNGT 1 cut(s) 316
HpyCH4IV ACGT 1 cut(s) 468
HpyCH4V TGCA 7 cut(s) 11, 38, 118, 124, 157, 321, 392
HpyF10VI GCNNNNNNNGC 2 cut(s) 494, 503
HpySE526I ACGT 1 cut(s) 468
Hsp92II CATG 3 cut(s) 401, 487, 497
KspI CCGCGG 2 cut(s) 54, 310
Lsp1109I GCAGC 2 cut(s) 50, 141
LweI GCATC 1 cut(s) 379
MaeI CTAG 1 cut(s) 480
MaeII ACGT 1 cut(s) 468
MaeIII GTNAC 1 cut(s) 469
MboII GAAGA 2 cut(s) 77, 88
MfeI CAATTG 2 cut(s) 119, 381
MlsI TGGCCA 2 cut(s) 26, 464
MluCI AATT 2 cut(s) 119, 381
MluNI TGGCCA 2 cut(s) 26, 464
MnlI CCTC 7 cut(s) 49, 282, 285, 365, 387, 419, 501
Mox20I TGGCCA 2 cut(s) 26, 464
Mph1103I ATGCAT 1 cut(s) 13
MscI TGGCCA 2 cut(s) 26, 464
MseI TTAA 1 cut(s) 141
Msp20I TGGCCA 2 cut(s) 26, 464
MspA1I CMGCKG 3 cut(s) 53, 309, 422
MunI CAATTG 2 cut(s) 119, 381
MvnI CGCG 2 cut(s) 53, 309
MwoI GCNNNNNNNGC 2 cut(s) 494, 503
NcoI CCATGG 1 cut(s) 397
NlaIII CATG 3 cut(s) 401, 487, 497
NlaIV GGNNCC 2 cut(s) 340, 489
NmeAIII GCCGAG 1 cut(s) 74
NmuCI GTSAC 1 cut(s) 469
NsiI ATGCAT 1 cut(s) 13
PfeI GAWTC 1 cut(s) 112
PkrI GCNGC 2 cut(s) 40, 156
PspN4I GGNNCC 2 cut(s) 340, 489
PspPI GGNCC 1 cut(s) 374
PvuII CAGCTG 1 cut(s) 422
RsaI GTAC 2 cut(s) 267, 512
RsaNI GTAC 2 cut(s) 266, 511
SacII CCGCGG 2 cut(s) 54, 310
SaqAI TTAA 1 cut(s) 141
SatI GCNGC 2 cut(s) 39, 155
Sau96I GGNCC 1 cut(s) 374
SetI ASST 8 cut(s) 48, 274, 283, 333, 424, 445, 471, 493
SfaNI GCATC 1 cut(s) 379
Sfr303I CCGCGG 2 cut(s) 54, 310
SgrBI CCGCGG 2 cut(s) 54, 310
Sse9I AATT 2 cut(s) 119, 381
SsiI CCGC 5 cut(s) 16, 51, 53, 307, 309
SspMI CTAG 1 cut(s) 480
StyI CCWWGG 1 cut(s) 397
TaaI ACNGT 1 cut(s) 316
TaiI ACGT 1 cut(s) 471
TasI AATT 2 cut(s) 119, 381
TatI WGTACW 1 cut(s) 510
TfiI GAWTC 1 cut(s) 112
Tru1I TTAA 1 cut(s) 141
Tru9I TTAA 1 cut(s) 141
TscAI CASTG 1 cut(s) 34
TseFI GTSAC 1 cut(s) 469
TseI GCWGC 2 cut(s) 38, 154
Tsp45I GTSAC 1 cut(s) 469
TspDTI ATGAA 2 cut(s) 237, 273
TspGWI ACGGA 1 cut(s) 124
TspRI CASTG 1 cut(s) 34
XspI CTAG 1 cut(s) 480
Zsp2I ATGCAT 1 cut(s) 13
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.