MD04G1071500.v1.1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000302
Physical Location & Seq
Reverse (-)
9688573 .. 9690885
2313 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1071500.v1.1.491

Sequence Viewer

Length: 2313 bp
ATGGAAAGCAAAAGGTTTGTGGCTTTCCTTCTTGGCTTGAGTTTCGCGTTATACTTATCTGTAGGAGGTGCTCATGAAGATGCAGCGCTAGAATCACTACCTGAGACAGATGACGAAGATATAAACATGCAAACTTATATTGTGTGGGTAAACCAACCAGTGCAGAAGTTCCTTTTTTCAAAATCTCATCAAGATTTGGAGAGTTGGTATCACACATTTTTGCCGACAACTATATCAAGCTCAAACAAGATGGTGAATCCACGGATGGTTCATGTATACCGCAATGTGGCCACCGGGTTTGCAGCAAAGCTCACTCCGGAGGAAGTAAAGGCCATGGAAAAGAAAGAGGGGTTTGTGTCCGCTCATCCAGAGCGAATTCTACCTCTGCACACTACTCACAGTCCTAACTTCTTGGGATTGAACCAAGGACTGGGAGTATGGAAAGGAGCAAACTACGGTAAAGGTGTAATAATCGGAGTTCTTGATACTGGGATAACACCTGATCATCCATCATTTAGCGATGAAGGAATGCCCCCTCCTCCAGCAAAATGGAAAGGCAAGTGTGACTTTAATGGAACAGTGTGCAATAACAAGCTTATTGGGGCAAGAAATTTCCAAGGCGGGAAAAGTACAGGAGCTCCTCCAGTTGACGATGAAGGTCATGGCACACACACGTCCAGCACAGCTGGAGGAAATTTTGTCAAAGGTGTCAGCGTCTTTGGTCAGGCCTATGGCACAGCATCGGGCATGGCACCTTACGCACATGTGGCAATGTACAAGGTCTGCGGGGAGGAGGGCTGTTCTGAAAGTAACATCTTAGCTGCAATGGATACTGCTGTCGAAGATGGTGTGGACGTGCTTTCCCTCTCACTTGGAGGTCCATCATTTCCTTTCTATGCTGATGGGATTGCAATTGGTGCATTTGGAGCAATTCAAAAGGGAATCTTTGTCAGTTGTTCAGCTGGAAACTCTGGTCCTTTCTATGGTAGTTTATCAAACGAGGCTCCGTGGATTCTCACAGTTGGAGCAAGCACCATTGACAGAAGCATAAGAGCAGTAGCAAAGCTAGGAAATGGGGGAGAATACGACGGTGAATCATTATTCCAGCCTAAAGATTTTGGTTCAGAGTTATTACCTATGGTGTATGCAGGCGCAAATGGCAAGGAATCATCTAGATTCTGCTCTTCCGGATCACTTGAAAATGTTGAAGGGGCAATAGTGGTGTGTGAGAGAGGTGGAGGAATAGGAAGAATCGACAAAGGGGCAGAAGTGAAAAGAGCTGGTGGAGCTGCCATGATTCTCGTGAATGCAGTGACTGATGGTGATAGCACCTTAGCTGACCCTCATGTGCTTCCCGCAACGCATGTGGGTTATGCTGCAGGTGTGAGAATCAAAGCCTACTTAAACTCCACCTCCAGTCCTGCAGCCACAATCTTGTTCAAAGGAACTATCATCGGAGATAAACTTGCTCCCAAAATCACTTCTTTTTCATCAAGGGGACCGAATATGGAAAGCCCTGGAATTTTGAAGCCCGACATCGTTGGTCCCGGTGTTAGCATCCTAGCGGCGTGGCCCGTTTCAGTGGACAATGCCACGGATTCTAACGCGACATTTAACATGATTTCAGGTACCTCAATGTCATGCCCACACCTAAGCGGCATTGCAGCCTTGCTCAAGAGTTCCCACCCTGACTGGTCACCGGCTGCCATTAAGTCTGCAATTATGACAACCGCGGGAGTAAATAACCTTGGAGGAAAGCCCATTGTTGATGAAACACTTAAGCCGGCAGACATCTTTGCCACTGGTGCAGGCCATGTTAACCCTTACAAAGCAAATGACCCTGGGCTCATCTACGACACAAAGCCAGCGGATTACATTCCTTACTTGTGTGGATTGAACTACACAAACGAACAGATACAAGTCATCACCCAACAAACAGTAAACTGCTCTGAAGTAGAAAACATACCAGAAGCACAGCTAAACTATCCCTCATTTTCTATTATAGTTGGGTCATCTGATGAGTCTAAGTCTCAGTACTATACAAGGACAGTGAAGAATGTTGGCTTGGCTAACTCAACTTACAAGTTGGATCTTAATGTACCACGTCAAATGGGAATGAGTGTGAATCCTGAGGTGCTCACATTCACAGACGTTAACCAGGAGATTACGTTCTATGTGGAGTTTCTCGCAGAAGACGGCGCTGGAAAGGATGGAGTTCCGTTTGCTCAGGGATATTTGAGATGGGTTTCTGATAAGCATTCTGTTAGTAGTCCAATATCAGTCATCTTTGACACCGGCTCGAATGGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

771

Amino Acids

81.37

Weight (kDa)

5.34

Isoelectric Point (pI)

31.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 45 - 130 2.3e-10 Peptidase inhibitor I9
Peptidase_S8 PF00082 153 - 581 4.5e-49 Subtilase family
PA PF02225 379 - 463 5.1e-13 PA domain
fn3_6 PF17766 659 - 761 5.2e-24 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1370
Acc36I ACCTGC 1 cut(s) 1370
Acc65I GGTACC 1 cut(s) 1628
AccB1I GGYRCC 2 cut(s) 751, 1628
AccB7I CCANNNNNTGG 1 cut(s) 430
AccBSI CCGCTC 1 cut(s) 362
AccI GTMKAC 1 cut(s) 276
AccII CGCG 3 cut(s) 47, 1607, 1733
AccIII TCCGGA 2 cut(s) 316, 1187
AclWI GGATC 2 cut(s) 1198, 2097
AcoI YGGCCR 1 cut(s) 288
AcsI RAATTY 4 cut(s) 375, 610, 694, 1521
AcuI CTGAAG 1 cut(s) 1971
AfaI GTAC 5 cut(s) 631, 776, 1630, 2036, 2100
AfeI AGCGCT 1 cut(s) 87
AfiI CCNNNNNNNGG 3 cut(s) 286, 430, 983
AflII CTTAAG 1 cut(s) 1778
AflIII ACRYGT 2 cut(s) 672, 763
AgsI TTSAA 8 cut(s) 180, 421, 935, 1199, 1208, 1441, 1528, 1897
AjiI CACGTC 3 cut(s) 675, 856, 2105
AjnI CCWGG 3 cut(s) 1516, 1840, 2157
AjuI GAANNNNNNNTTGG 2 cut(s) 2048, 2080
AloI GAACNNNNNNTCC 2 cut(s) 2153, 2185
Alw21I GWGCWC 3 cut(s) 73, 640, 2139
Alw26I GTCTC 2 cut(s) 98, 2034
AlwI GGATC 2 cut(s) 1198, 2097
AlwNI CAGNNNCTG 1 cut(s) 1316
Aor13HI TCCGGA 2 cut(s) 316, 1187
Aor51HI AGCGCT 1 cut(s) 87
AoxI GGCC 5 cut(s) 288, 330, 726, 1571, 1810
ApeKI GCWGC 8 cut(s) 83, 302, 821, 1289, 1376, 1424, 1664, 1703
ApoI RAATTY 4 cut(s) 375, 610, 694, 1521
Asp718I GGTACC 1 cut(s) 1628
AspLEI GCGC 3 cut(s) 88, 1154, 2201
AspS9I GGNCC 5 cut(s) 878, 974, 1499, 1544, 1572
AsuC2I CCSGG 2 cut(s) 295, 1548
AsuHPI GGTGA 5 cut(s) 265, 1103, 1334, 1689, 1918
AvaII GGWCC 4 cut(s) 878, 974, 1499, 1544
AxyI CCTNAGG 1 cut(s) 2130
BaeI ACNNNNGTAYC 2 cut(s) 822, 855
BalI TGGCCA 1 cut(s) 290
BanI GGYRCC 2 cut(s) 751, 1628
BanII GRGCYC 2 cut(s) 640, 1848
BauI CACGAG 1 cut(s) 1301
BbsI GAAGAC 1 cut(s) 2199
Bbv12I GWGCWC 3 cut(s) 73, 640, 2139
BbvI GCAGC 8 cut(s) 95, 314, 808, 1276, 1363, 1436, 1676, 1690
BccI CCATC 9 cut(s) 244, 259, 517, 839, 889, 896, 1313, 2204, 2235
BceAI ACGGC 1 cut(s) 2212
BciT130I CCWGG 3 cut(s) 1518, 1842, 2159
BciVI GTATCC 1 cut(s) 823
BclI TGATCA 1 cut(s) 502
BcnI CCSGG 2 cut(s) 295, 1548
BcoDI GTCTC 2 cut(s) 98, 2034
BfaI CTAG 4 cut(s) 89, 1067, 1173, 1562
BfmI CTRYAG 3 cut(s) 60, 1377, 1422
BfoI RGCGCY 2 cut(s) 89, 2202
BfrI CTTAAG 1 cut(s) 1778
BfuAI ACCTGC 1 cut(s) 1370
BfuI GTATCC 1 cut(s) 823
BmcAI AGTACT 1 cut(s) 2036
Bme1390I CCNGG 5 cut(s) 295, 1518, 1548, 1842, 2159
Bme18I GGWCC 4 cut(s) 878, 974, 1499, 1544
BmgBI CACGTC 3 cut(s) 675, 856, 2105
BmgT120I GGNCC 5 cut(s) 878, 974, 1499, 1544, 1572
BmiI GGNNCC 5 cut(s) 753, 1005, 1500, 1546, 1630
BmrFI CCNGG 5 cut(s) 295, 1518, 1548, 1842, 2159
BmrI ACTGGG 2 cut(s) 440, 498
BmsI GCATC 3 cut(s) 70, 749, 1566
BmuI ACTGGG 2 cut(s) 440, 498
BpiI GAAGAC 1 cut(s) 2199
BpmI CTGGAG 4 cut(s) 525, 627, 708, 1399
Bpu10I CCTNAGC 3 cut(s) 1333, 1652, 2226
BpuEI CTTGAG 2 cut(s) 58, 1658
BpuMI CCSGG 2 cut(s) 295, 1548
BsaWI WCCGGW 2 cut(s) 316, 1187
Bsc4I CCNNNNNNNGG 3 cut(s) 286, 430, 983
Bse118I RCCGGY 3 cut(s) 1699, 1783, 2294
Bse1I ACTGG 7 cut(s) 158, 435, 493, 644, 1416, 1697, 1807
Bse21I CCTNAGG 1 cut(s) 2130
Bse3DI GCAATG 4 cut(s) 289, 777, 831, 1659
BseAI TCCGGA 2 cut(s) 316, 1187
BseBI CCWGG 3 cut(s) 1518, 1842, 2159
BseGI GGATG 5 cut(s) 270, 364, 505, 1557, 2215
BseLI CCNNNNNNNGG 3 cut(s) 286, 430, 983
BseMI GCAATG 4 cut(s) 289, 777, 831, 1659
BseMII CTCAG 4 cut(s) 93, 2045, 2121, 2240
BseNI ACTGG 7 cut(s) 158, 435, 493, 644, 1416, 1697, 1807
BseRI GAGGAG 3 cut(s) 528, 630, 806
BseXI GCAGC 8 cut(s) 95, 314, 808, 1276, 1363, 1436, 1676, 1690
BsgI GTGCAG 3 cut(s) 182, 371, 1827
Bsh1236I CGCG 3 cut(s) 47, 1607, 1733
BshFI GGCC 5 cut(s) 290, 332, 728, 1573, 1812
BshNI GGYRCC 2 cut(s) 751, 1628
BsiHKAI GWGCWC 3 cut(s) 73, 640, 2139
BsiSI CCGG 7 cut(s) 294, 317, 1188, 1548, 1700, 1784, 2295
BslFI GGGAC 2 cut(s) 1512, 1530
BslI CCNNNNNNNGG 3 cut(s) 286, 430, 983
BsmAI GTCTC 2 cut(s) 98, 2034
BsmFI GGGAC 2 cut(s) 1512, 1530
BsmI GAATGC 3 cut(s) 534, 1312, 2257
BsnI GGCC 5 cut(s) 290, 332, 728, 1573, 1812
Bsp1286I GDGCHC 4 cut(s) 73, 640, 1848, 2139
Bsp13I TCCGGA 2 cut(s) 316, 1187
Bsp1407I TGTACA 1 cut(s) 774
Bsp143I GATC 3 cut(s) 502, 1190, 2089
Bsp19I CCATGG 1 cut(s) 333
BspANI GGCC 5 cut(s) 290, 332, 728, 1573, 1812
BspCNI CTCAG 4 cut(s) 94, 2044, 2122, 2239
BspEI TCCGGA 2 cut(s) 316, 1187
BspFNI CGCG 3 cut(s) 47, 1607, 1733
BspHI TCATGA 1 cut(s) 73
BspLI GGNNCC 5 cut(s) 753, 1005, 1500, 1546, 1630
BspMAI CTGCAG 2 cut(s) 1381, 1426
BspMI ACCTGC 1 cut(s) 1370
BspPI GGATC 2 cut(s) 1198, 2097
BspQI GCTCTTC 1 cut(s) 1189
BspT107I GGYRCC 2 cut(s) 751, 1628
BspTI CTTAAG 1 cut(s) 1778
BsrBI CCGCTC 1 cut(s) 362
BsrDI GCAATG 4 cut(s) 289, 777, 831, 1659
BsrFI RCCGGY 3 cut(s) 1699, 1783, 2294
BsrGI TGTACA 1 cut(s) 774
BsrI ACTGG 7 cut(s) 158, 435, 493, 644, 1416, 1697, 1807
BssAI RCCGGY 3 cut(s) 1699, 1783, 2294
BssMI GATC 3 cut(s) 502, 1190, 2089
BssNAI GTATAC 1 cut(s) 277
BssSI CACGAG 1 cut(s) 1301
BssT1I CCWWGG 4 cut(s) 333, 424, 616, 1747
Bst1107I GTATAC 1 cut(s) 277
Bst2BI CACGAG 1 cut(s) 1301
Bst2UI CCWGG 3 cut(s) 1518, 1842, 2159
Bst4CI ACNGT 7 cut(s) 401, 458, 580, 1021, 1091, 1939, 2050
Bst6I CTCTTC 1 cut(s) 1189
BstAFI CTTAAG 1 cut(s) 1778
BstAPI GCANNNNNTGC 1 cut(s) 917
BstAUI TGTACA 1 cut(s) 774
BstC8I GCNNGC 5 cut(s) 1030, 1150, 1785, 1810, 1866
BstDEI CTNAG 8 cut(s) 102, 817, 1333, 1652, 2025, 2031, 2130, 2226
BstDSI CCRYGG 5 cut(s) 260, 333, 1007, 1593, 1731
BstEII GGTNACC 1 cut(s) 1695
BstF5I GGATG 5 cut(s) 270, 364, 505, 1557, 2215
BstFNI CGCG 3 cut(s) 47, 1607, 1733
BstH2I RGCGCY 2 cut(s) 89, 2202
BstHHI GCGC 3 cut(s) 88, 1154, 2201
BstKTI GATC 3 cut(s) 505, 1193, 2092
BstMAI GTCTC 2 cut(s) 98, 2034
BstMBI GATC 3 cut(s) 502, 1190, 2089
BstMWI GCNNNNNNNGC 7 cut(s) 758, 767, 917, 926, 1158, 1286, 1805
BstNI CCWGG 3 cut(s) 1518, 1842, 2159
BstNSI RCATGY 3 cut(s) 130, 767, 1367
BstPI GGTNACC 1 cut(s) 1695
BstSCI CCNGG 5 cut(s) 293, 1516, 1546, 1840, 2157
BstSFI CTRYAG 3 cut(s) 60, 1377, 1422
BstUI CGCG 3 cut(s) 47, 1607, 1733
BstV1I GCAGC 8 cut(s) 95, 314, 808, 1276, 1363, 1436, 1676, 1690
BstV2I GAAGAC 1 cut(s) 2199
BstX2I RGATCY 1 cut(s) 2089
BstXI CCANNNNNNTGG 1 cut(s) 549
BstYI RGATCY 1 cut(s) 2089
BstZ17I GTATAC 1 cut(s) 277
Bsu36I CCTNAGG 1 cut(s) 2130
BsuI GTATCC 1 cut(s) 823
BsuRI GGCC 5 cut(s) 290, 332, 728, 1573, 1812
BtgI CCRYGG 5 cut(s) 260, 333, 1007, 1593, 1731
BtgZI GCGATG 1 cut(s) 534
BtrI CACGTC 3 cut(s) 675, 856, 2105
BtsCI GGATG 5 cut(s) 270, 364, 505, 1557, 2215
BtsI GCAGTG 1 cut(s) 1317
BtsIMutI CAGTG 6 cut(s) 165, 585, 1317, 1587, 1800, 2055
BveI ACCTGC 1 cut(s) 1370
Cac8I GCNNGC 5 cut(s) 1030, 1150, 1785, 1810, 1866
CaiI CAGNNNCTG 1 cut(s) 1316
CciI TCATGA 1 cut(s) 73
CfoI GCGC 3 cut(s) 88, 1154, 2201
Cfr10I RCCGGY 3 cut(s) 1699, 1783, 2294
Cfr13I GGNCC 5 cut(s) 878, 974, 1499, 1544, 1572
Cfr42I CCGCGG 1 cut(s) 1734
CseI GACGC 1 cut(s) 703
Csp6I GTAC 5 cut(s) 630, 775, 1629, 2035, 2099
CspCI CAANNNNNGTGG 4 cut(s) 280, 315, 1347, 1382
CviQI GTAC 5 cut(s) 630, 775, 1629, 2035, 2099
DdeI CTNAG 8 cut(s) 102, 817, 1333, 1652, 2025, 2031, 2130, 2226
DpnI GATC 3 cut(s) 504, 1192, 2091
DpnII GATC 3 cut(s) 502, 1190, 2089
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 1 cut(s) 1189
EarI CTCTTC 1 cut(s) 1189
Ecl136II GAGCTC 1 cut(s) 638
Eco130I CCWWGG 4 cut(s) 333, 424, 616, 1747
Eco147I AGGCCT 1 cut(s) 728
Eco24I GRGCYC 2 cut(s) 640, 1848
Eco47I GGWCC 4 cut(s) 878, 974, 1499, 1544
Eco47III AGCGCT 1 cut(s) 87
Eco53kI GAGCTC 1 cut(s) 638
Eco57I CTGAAG 1 cut(s) 1971
Eco81I CCTNAGG 1 cut(s) 2130
Eco91I GGTNACC 1 cut(s) 1695
EcoICRI GAGCTC 1 cut(s) 638
EcoO65I GGTNACC 1 cut(s) 1695
EcoRI GAATTC 1 cut(s) 375
EcoRII CCWGG 3 cut(s) 1516, 1840, 2157
EcoT14I CCWWGG 4 cut(s) 333, 424, 616, 1747
EcoT38I GRGCYC 2 cut(s) 640, 1848
ErhI CCWWGG 4 cut(s) 333, 424, 616, 1747
FalI AAGNNNNNCTT 4 cut(s) 551, 583, 929, 961
FaqI GGGAC 2 cut(s) 1512, 1530
FauI CCCGC 4 cut(s) 614, 779, 1363, 1726
FbaI TGATCA 1 cut(s) 502
FblI GTMKAC 1 cut(s) 276
FokI GGATG 5 cut(s) 277, 351, 492, 1544, 2222
FriOI GRGCYC 2 cut(s) 640, 1848
FspBI CTAG 4 cut(s) 89, 1067, 1173, 1562
GlaI GCGC 3 cut(s) 87, 1153, 2200
GsuI CTGGAG 4 cut(s) 525, 627, 708, 1399
HaeII RGCGCY 2 cut(s) 89, 2202
HaeIII GGCC 5 cut(s) 290, 332, 728, 1573, 1812
HapII CCGG 7 cut(s) 294, 317, 1188, 1548, 1700, 1784, 2295
HgaI GACGC 1 cut(s) 703
HhaI GCGC 3 cut(s) 88, 1154, 2201
Hin6I GCGC 3 cut(s) 86, 1152, 2199
HinP1I GCGC 3 cut(s) 86, 1152, 2199
HincII GTYRAC 3 cut(s) 649, 1819, 2155
HindII GTYRAC 3 cut(s) 649, 1819, 2155
HindIII AAGCTT 1 cut(s) 593
HpaI GTTAAC 2 cut(s) 1819, 2155
HpaII CCGG 7 cut(s) 294, 317, 1188, 1548, 1700, 1784, 2295
HphI GGTGA 5 cut(s) 265, 1103, 1334, 1689, 1918
Hpy166II GTNNAC 8 cut(s) 151, 277, 649, 853, 1585, 1819, 1942, 2155
Hpy188I TCNGA 7 cut(s) 476, 805, 1126, 1457, 1951, 2017, 2251
Hpy8I GTNNAC 8 cut(s) 151, 277, 649, 853, 1585, 1819, 1942, 2155
Hpy99I CGWCG 1 cut(s) 1091
HpyAV CCTTC 4 cut(s) 38, 518, 650, 1202
HpyCH4III ACNGT 7 cut(s) 401, 458, 580, 1021, 1091, 1939, 2050
HpyCH4IV ACGT 5 cut(s) 674, 855, 2104, 2151, 2168
HpyF10VI GCNNNNNNNGC 7 cut(s) 758, 767, 917, 926, 1158, 1286, 1805
HpyF3I CTNAG 8 cut(s) 102, 817, 1333, 1652, 2025, 2031, 2130, 2226
HpySE526I ACGT 5 cut(s) 674, 855, 2104, 2151, 2168
HspAI GCGC 3 cut(s) 86, 1152, 2199
Kpn2I TCCGGA 2 cut(s) 316, 1187
KpnI GGTACC 1 cut(s) 1632
KroI GCCGGC 1 cut(s) 1783
KroNI GCCGGC 1 cut(s) 1785
Ksp22I TGATCA 1 cut(s) 502
KspAI GTTAAC 2 cut(s) 1819, 2155
KspI CCGCGG 1 cut(s) 1734
Kzo9I GATC 3 cut(s) 502, 1190, 2089
LguI GCTCTTC 1 cut(s) 1189
LmnI GCTCC 8 cut(s) 446, 635, 643, 926, 1009, 1025, 1286, 1474
Lsp1109I GCAGC 8 cut(s) 95, 314, 808, 1276, 1363, 1436, 1676, 1690
LweI GCATC 3 cut(s) 70, 749, 1566
MaeI CTAG 4 cut(s) 89, 1067, 1173, 1562
MaeII ACGT 5 cut(s) 674, 855, 2104, 2151, 2168
MaeIII GTNAC 4 cut(s) 563, 809, 1312, 1695
MalI GATC 3 cut(s) 504, 1192, 2091
MbiI CCGCTC 1 cut(s) 362
MboI GATC 3 cut(s) 502, 1190, 2089
MboII GAAGA 7 cut(s) 89, 128, 854, 1176, 1260, 2065, 2204
MfeI CAATTG 1 cut(s) 912
MflI RGATCY 1 cut(s) 2089
MhlI GDGCHC 4 cut(s) 73, 640, 1848, 2139
MlsI TGGCCA 1 cut(s) 290
MluCI AATT 7 cut(s) 375, 610, 694, 912, 930, 1521, 1719
MluNI TGGCCA 1 cut(s) 290
MlyI GAGTC 1 cut(s) 2030
MmeI TCCRAC 2 cut(s) 1003, 2067
Mox20I TGGCCA 1 cut(s) 290
MroI TCCGGA 2 cut(s) 316, 1187
MroNI GCCGGC 1 cut(s) 1783
MscI TGGCCA 1 cut(s) 290
MseI TTAA 8 cut(s) 570, 1403, 1614, 1710, 1779, 1818, 2094, 2154
MslI CAYNNNNRTG 1 cut(s) 78
Msp20I TGGCCA 1 cut(s) 290
MspA1I CMGCKG 4 cut(s) 686, 962, 1733, 1868
MspCI CTTAAG 1 cut(s) 1778
MspI CCGG 7 cut(s) 294, 317, 1188, 1548, 1700, 1784, 2295
MspR9I CCNGG 5 cut(s) 295, 1518, 1548, 1842, 2159
MunI CAATTG 1 cut(s) 912
Mva1269I GAATGC 3 cut(s) 534, 1312, 2257
MvaI CCWGG 3 cut(s) 1518, 1842, 2159
MvnI CGCG 3 cut(s) 47, 1607, 1733
MwoI GCNNNNNNNGC 7 cut(s) 758, 767, 917, 926, 1158, 1286, 1805
NaeI GCCGGC 1 cut(s) 1785
NciI CCSGG 2 cut(s) 295, 1548
NcoI CCATGG 1 cut(s) 333
NdeII GATC 3 cut(s) 502, 1190, 2089
NgoMIV GCCGGC 1 cut(s) 1783
NlaIV GGNNCC 5 cut(s) 753, 1005, 1500, 1546, 1630
NmuCI GTSAC 3 cut(s) 563, 1312, 1695
NspI RCATGY 3 cut(s) 130, 767, 1367
PagI TCATGA 1 cut(s) 73
PaqCI CACCTGC 1 cut(s) 1370
PasI CCCWGGG 1 cut(s) 1841
PceI AGGCCT 1 cut(s) 728
PciI ACATGT 1 cut(s) 763
PciSI GCTCTTC 1 cut(s) 1189
PctI GAATGC 3 cut(s) 534, 1312, 2257
PdiI GCCGGC 1 cut(s) 1785
PflMI CCANNNNNTGG 1 cut(s) 430
PleI GAGTC 1 cut(s) 2029
PpsI GAGTC 1 cut(s) 2029
PscI ACATGT 1 cut(s) 763
Psp124BI GAGCTC 1 cut(s) 640
Psp6I CCWGG 3 cut(s) 1516, 1840, 2157
PspEI GGTNACC 1 cut(s) 1695
PspGI CCWGG 3 cut(s) 1516, 1840, 2157
PspN4I GGNNCC 5 cut(s) 753, 1005, 1500, 1546, 1630
PspPI GGNCC 5 cut(s) 878, 974, 1499, 1544, 1572
PstI CTGCAG 2 cut(s) 1381, 1426
PstNI CAGNNNCTG 1 cut(s) 1316
PsuI RGATCY 1 cut(s) 2089
PvuII CAGCTG 2 cut(s) 686, 962
RsaI GTAC 5 cut(s) 631, 776, 1630, 2036, 2100
RsaNI GTAC 5 cut(s) 630, 775, 1629, 2035, 2099
RseI CAYNNNNRTG 1 cut(s) 78
SacI GAGCTC 1 cut(s) 640
SacII CCGCGG 1 cut(s) 1734
SapI GCTCTTC 1 cut(s) 1189
SaqAI TTAA 8 cut(s) 570, 1403, 1614, 1710, 1779, 1818, 2094, 2154
Sau3AI GATC 3 cut(s) 502, 1190, 2089
Sau96I GGNCC 5 cut(s) 878, 974, 1499, 1544, 1572
ScaI AGTACT 1 cut(s) 2036
SchI GAGTC 1 cut(s) 2030
ScrFI CCNGG 5 cut(s) 295, 1518, 1548, 1842, 2159
SduI GDGCHC 4 cut(s) 73, 640, 1848, 2139
SfaNI GCATC 3 cut(s) 70, 749, 1566
SfcI CTRYAG 3 cut(s) 60, 1377, 1422
Sfr303I CCGCGG 1 cut(s) 1734
SgrBI CCGCGG 1 cut(s) 1734
SinI GGWCC 4 cut(s) 878, 974, 1499, 1544
SmiMI CAYNNNNRTG 1 cut(s) 78
SmlI CTYRAG 3 cut(s) 37, 1673, 1778
SmoI CTYRAG 3 cut(s) 37, 1673, 1778
Sse9I AATT 7 cut(s) 375, 610, 694, 912, 930, 1521, 1719
SseBI AGGCCT 1 cut(s) 728
SspMI CTAG 4 cut(s) 89, 1067, 1173, 1562
SstI GAGCTC 1 cut(s) 640
StuI AGGCCT 1 cut(s) 728
StyD4I CCNGG 5 cut(s) 293, 1516, 1546, 1840, 2157
StyI CCWWGG 4 cut(s) 333, 424, 616, 1747
TaaI ACNGT 7 cut(s) 401, 458, 580, 1021, 1091, 1939, 2050
TaiI ACGT 5 cut(s) 677, 858, 2107, 2154, 2171
TaqI TCGA 3 cut(s) 840, 1254, 2300
TaqII GACCGA 1 cut(s) 1516
TasI AATT 7 cut(s) 375, 610, 694, 912, 930, 1521, 1719
TatI WGTACW 3 cut(s) 629, 774, 2034
TauI GCSGC 2 cut(s) 1568, 1659
Tru1I TTAA 8 cut(s) 570, 1403, 1614, 1710, 1779, 1818, 2094, 2154
Tru9I TTAA 8 cut(s) 570, 1403, 1614, 1710, 1779, 1818, 2094, 2154
TscAI CASTG 6 cut(s) 165, 585, 1317, 1587, 1807, 2055
TseFI GTSAC 3 cut(s) 563, 1312, 1695
TseI GCWGC 8 cut(s) 83, 302, 821, 1289, 1376, 1424, 1664, 1703
Tsp45I GTSAC 3 cut(s) 563, 1312, 1695
TspDTI ATGAA 6 cut(s) 90, 260, 537, 669, 1479, 1785
TspGWI ACGGA 4 cut(s) 277, 996, 1610, 2208
TspRI CASTG 6 cut(s) 165, 585, 1317, 1587, 1807, 2055
Van91I CCANNNNNTGG 1 cut(s) 430
Vha464I CTTAAG 1 cut(s) 1778
VpaK11BI GGWCC 4 cut(s) 878, 974, 1499, 1544
XapI RAATTY 4 cut(s) 375, 610, 694, 1521
XbaI TCTAGA 1 cut(s) 1172
XceI RCATGY 3 cut(s) 130, 767, 1367
XmiI GTMKAC 1 cut(s) 276
XspI CTAG 4 cut(s) 89, 1067, 1173, 1562
ZrmI AGTACT 1 cut(s) 2036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.