MD04G1071200.v1.1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000302
Physical Location & Seq
Reverse (-)
9677625 .. 9679322
1698 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1071200.v1.1.491

Sequence Viewer

Length: 1446 bp
ATGCCAAAGGATGGTGTTGATGTGATCTCAATCTCCCTTGGTGAAGCGTCAGTTCCATTTTTCCAAGACACCACTGCAATAGGCTCATTTGCAGCAATCCAAAAAGGAATATTTGTAAGTTGTGCAGCTGGAAATTCTGGCCCATTTAACGGCACACTATCTAATGAAGCCCCCTGGATGCTAACAGTTGGAGCAAGCACAATAGACAGACATGTTGTAGCCACAGCAAAACTTGGAAATGGACAAGTATTTGATGGCGAATCTCTCTTTCAGCCCAGTGATTTCCCTTCAACATTAATGCCTCTTATTTATGCTGGAGTAAATGGTAATGATTCGGCATTGTGTGCTGAAGGATCCTTGAAAGGTCTCCCTGTTAAAGGTAAGGTAGTTGTGTGTGAGCGAGGGGGAGGTATAGGAAGGATTGCTAAGGGAGAAGAAGTGAAAAACGCTGGAGGCGCTGCCATGATTCTTTTGAATGAAGAAACTGACGGTGTCAGTATTTCAGCTGATGTTCATGTTCTTCCCGCAACACATGTGAGCTACGCAGCTGGACTGAAGATCAAAGCATATATAAATTCAACAGTAACACCAACAGCAACAATCTTATTCAAAGGAACTGTCATCGGAGACTCATCTACTCCAGTGGTTGCTTCCTTCTCTTCAAGAGGCCCCAGCCTGGCAAGTCCAGGTATTCTGAAACCAGACATTATTGGTCCTGGAGTAAGCATTCTTGCTGCATGGCCATTCCCCGTTGACAATACCACAAAATCAAAAATCAATTTCAACATAATGTCTGGAACTTCAATGTCTTGCCCGCATCTCAGTGGAATTGCAGCTTTGCTTAAGAGCTCACACCCTTACTGGTCACCAGCTGCCATTAAATCTGCTATAATGACTTCTGCTGATCTTCTAAATTTAGAAGGCAAACCTATTCTCGATGAACAACTTCAGCCTGCAGATGTTTTAGCCACTGGTGCAGGCCAGGTCAACCCATCAAAGGCAAATGACCCGGGATTAATTTATGATATTCAACCTGATGATTATATCCCTTATCTTTGTGGCTTGGGCTATAAAGATGATGAAATCAGTATCATTGTGCATAGACAAATAAAATGCTCAATGGTATCGAGCATACCTGAAGGAGAGCTGAACTACCCTTCGTTTTCTGTCACACTGGGACCATCTGAGACATTCACACGAACTCTGACAAATGTTGGTGAGGCATATTCATCTTATGCAGTCAAGGTTAATGCACCAGAAGGAGTACATGTGAGTGTCAAGCCCAAAACACTTAACTTCACAAAGGTGAACCAGAAGATGTCGTATTCAGTGACATTCAGCCATATTGGTTCAAAAGGTGAAGCTGGTGAATTCACTCGAGGATTCTTAACGTGGGTTTCTGCTAAATATGTTGTTAGGAGTCCGGTTTCTGTTAAATTACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

482

Amino Acids

50.34

Weight (kDa)

6.09

Isoelectric Point (pI)

32.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 3 - 302 1.8e-25 Subtilase family
PA PF02225 101 - 187 3.9e-13 PA domain
fn3_6 PF17766 382 - 478 3.1e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 11
AciI CCGC 2 cut(s) 525, 815
AclWI GGATC 2 cut(s) 348, 361
AcoI YGGCCR 1 cut(s) 740
AcsI RAATTY 4 cut(s) 133, 574, 913, 1370
AcuI CTGAAG 4 cut(s) 369, 575, 932, 1158
AfaI GTAC 1 cut(s) 1266
AfiI CCNNNNNNNGG 5 cut(s) 11, 149, 377, 676, 997
AflII CTTAAG 1 cut(s) 842
AflIII ACRYGT 3 cut(s) 211, 532, 1267
AjnI CCWGG 5 cut(s) 173, 675, 685, 715, 981
AleI CACNNNNGTG 1 cut(s) 1304
AluBI AGCT 9 cut(s) 128, 506, 540, 548, 836, 849, 872, 1147, 1364
AluI AGCT 9 cut(s) 128, 506, 540, 548, 836, 849, 872, 1147, 1364
Alw21I GWGCWC 1 cut(s) 851
Alw26I GTCTC 3 cut(s) 371, 621, 1181
AlwI GGATC 2 cut(s) 348, 361
Ama87I CYCGRG 2 cut(s) 1009, 1377
AoxI GGCC 4 cut(s) 139, 667, 740, 979
ApeKI GCWGC 7 cut(s) 92, 125, 458, 545, 734, 833, 872
ApoI RAATTY 4 cut(s) 133, 574, 913, 1370
ArsI GACNNNNNNTTYG 2 cut(s) 603, 635
AseI ATTAAT 2 cut(s) 296, 1016
Asp700I GAANNNNTTC 1 cut(s) 945
AspLEI GCGC 1 cut(s) 458
AspS9I GGNCC 4 cut(s) 140, 668, 713, 1178
AsuC2I CCSGG 2 cut(s) 1010, 1011
AsuHPI GGTGA 6 cut(s) 53, 858, 1229, 1318, 1370, 1379
AvaI CYCGRG 2 cut(s) 1009, 1377
AvaII GGWCC 2 cut(s) 713, 1178
BalI TGGCCA 1 cut(s) 742
BamHI GGATCC 1 cut(s) 353
BanII GRGCYC 1 cut(s) 851
Bbv12I GWGCWC 1 cut(s) 851
BbvI GCAGC 7 cut(s) 104, 137, 445, 557, 721, 845, 859
BccI CCATC 4 cut(s) 5, 248, 1000, 1189
BceAI ACGGC 1 cut(s) 166
BciT130I CCWGG 5 cut(s) 175, 677, 687, 717, 983
BcnI CCSGG 2 cut(s) 1010, 1011
BcoDI GTCTC 3 cut(s) 371, 621, 1181
BfmI CTRYAG 1 cut(s) 954
BfoI RGCGCY 1 cut(s) 459
BfrI CTTAAG 1 cut(s) 842
BisI GCNGC 7 cut(s) 93, 126, 459, 546, 735, 834, 873
BlsI GCNGC 7 cut(s) 94, 127, 460, 547, 736, 835, 874
Bme1390I CCNGG 7 cut(s) 175, 677, 687, 717, 983, 1010, 1011
Bme18I GGWCC 2 cut(s) 713, 1178
BmeT110I CYCGRG 2 cut(s) 1009, 1377
BmgT120I GGNCC 4 cut(s) 140, 668, 713, 1178
BmiI GGNNCC 3 cut(s) 355, 670, 1179
BmrFI CCNGG 7 cut(s) 175, 677, 687, 717, 983, 1010, 1011
BmrI ACTGGG 2 cut(s) 270, 1184
BmsI GCATC 2 cut(s) 168, 826
BmuI ACTGGG 2 cut(s) 270, 1184
BpmI CTGGAG 4 cut(s) 336, 471, 624, 738
Bpu10I CCTNAGC 1 cut(s) 426
BpuMI CCSGG 2 cut(s) 1010, 1011
BsaBI GATNNNNATC 1 cut(s) 29
BsaI GGTCTC 1 cut(s) 371
BsaJI CCNNGG 3 cut(s) 37, 173, 1009
BsaWI WCCGGW 1 cut(s) 1423
BsaXI ACNNNNNCTCC 4 cut(s) 423, 453, 1254, 1284
Bsc4I CCNNNNNNNGG 5 cut(s) 11, 149, 377, 676, 997
Bse1I ACTGG 5 cut(s) 276, 641, 866, 976, 1179
Bse8I GATNNNNATC 1 cut(s) 29
BseBI CCWGG 5 cut(s) 175, 677, 687, 717, 983
BseDI CCNNGG 3 cut(s) 37, 173, 1009
BseGI GGATG 2 cut(s) 16, 183
BseJI GATNNNNATC 1 cut(s) 29
BseLI CCNNNNNNNGG 5 cut(s) 11, 149, 377, 676, 997
BseMII CTCAG 2 cut(s) 835, 1176
BseNI ACTGG 5 cut(s) 276, 641, 866, 976, 1179
BseXI GCAGC 7 cut(s) 104, 137, 445, 557, 721, 845, 859
BseYI CCCAGC 1 cut(s) 671
BsgI GTGCAG 2 cut(s) 144, 996
BshFI GGCC 4 cut(s) 141, 669, 742, 981
BsiHKAI GWGCWC 1 cut(s) 851
BsiHKCI CYCGRG 2 cut(s) 1009, 1377
BsiSI CCGG 2 cut(s) 1010, 1424
BslFI GGGAC 1 cut(s) 1191
BslI CCNNNNNNNGG 5 cut(s) 11, 149, 377, 676, 997
BsmAI GTCTC 3 cut(s) 371, 621, 1181
BsmFI GGGAC 1 cut(s) 1191
BsmI GAATGC 1 cut(s) 726
BsnI GGCC 4 cut(s) 141, 669, 742, 981
Bso31I GGTCTC 1 cut(s) 371
BsoBI CYCGRG 2 cut(s) 1009, 1377
Bsp1286I GDGCHC 1 cut(s) 851
Bsp143I GATC 4 cut(s) 24, 353, 558, 904
BspACI CCGC 2 cut(s) 525, 815
BspANI GGCC 4 cut(s) 141, 669, 742, 981
BspCNI CTCAG 2 cut(s) 834, 1177
BspLI GGNNCC 3 cut(s) 355, 670, 1179
BspMAI CTGCAG 1 cut(s) 958
BspPI GGATC 2 cut(s) 348, 361
BspTI CTTAAG 1 cut(s) 842
BspTNI GGTCTC 1 cut(s) 371
BsrI ACTGG 5 cut(s) 276, 641, 866, 976, 1179
BssECI CCNNGG 3 cut(s) 37, 173, 1009
BssMI GATC 4 cut(s) 24, 353, 558, 904
BssT1I CCWWGG 1 cut(s) 37
Bst2UI CCWGG 5 cut(s) 175, 677, 687, 717, 983
Bst4CI ACNGT 5 cut(s) 187, 491, 583, 619, 1443
Bst6I CTCTTC 1 cut(s) 664
BstAFI CTTAAG 1 cut(s) 842
BstAPI GCANNNNNTGC 1 cut(s) 344
BstC8I GCNNGC 4 cut(s) 196, 815, 954, 979
BstDEI CTNAG 3 cut(s) 426, 821, 1185
BstEII GGTNACC 1 cut(s) 864
BstENI CCTNNNNNAGG 1 cut(s) 375
BstF5I GGATG 2 cut(s) 16, 183
BstH2I RGCGCY 1 cut(s) 459
BstHHI GCGC 1 cut(s) 458
BstKTI GATC 4 cut(s) 27, 356, 561, 907
BstMAI GTCTC 3 cut(s) 371, 621, 1181
BstMBI GATC 4 cut(s) 24, 353, 558, 904
BstMWI GCNNNNNNNGC 3 cut(s) 344, 455, 974
BstNI CCWGG 5 cut(s) 175, 677, 687, 717, 983
BstNSI RCATGY 3 cut(s) 215, 536, 1271
BstPI GGTNACC 1 cut(s) 864
BstSCI CCNGG 7 cut(s) 173, 675, 685, 715, 981, 1008, 1009
BstSFI CTRYAG 1 cut(s) 954
BstV1I GCAGC 7 cut(s) 104, 137, 445, 557, 721, 845, 859
BstX2I RGATCY 1 cut(s) 353
BstYI RGATCY 1 cut(s) 353
BsuRI GGCC 4 cut(s) 141, 669, 742, 981
BtsCI GGATG 2 cut(s) 16, 183
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 7 cut(s) 72, 283, 648, 829, 969, 1172, 1335
Cac8I GCNNGC 4 cut(s) 196, 815, 954, 979
CfoI GCGC 1 cut(s) 458
Cfr13I GGNCC 4 cut(s) 140, 668, 713, 1178
Cfr9I CCCGGG 1 cut(s) 1009
CseI GACGC 1 cut(s) 36
Csp6I GTAC 1 cut(s) 1265
CviAII CATG 6 cut(s) 212, 463, 515, 533, 738, 1268
CviQI GTAC 1 cut(s) 1265
DdeI CTNAG 3 cut(s) 426, 821, 1185
DpnI GATC 4 cut(s) 26, 355, 560, 906
DpnII GATC 4 cut(s) 24, 353, 558, 904
EaeI YGGCCR 1 cut(s) 740
Eam1104I CTCTTC 1 cut(s) 664
EarI CTCTTC 1 cut(s) 664
Ecl136II GAGCTC 1 cut(s) 849
Eco130I CCWWGG 1 cut(s) 37
Eco24I GRGCYC 1 cut(s) 851
Eco31I GGTCTC 1 cut(s) 371
Eco47I GGWCC 2 cut(s) 713, 1178
Eco53kI GAGCTC 1 cut(s) 849
Eco57I CTGAAG 4 cut(s) 369, 575, 932, 1158
Eco88I CYCGRG 2 cut(s) 1009, 1377
Eco91I GGTNACC 1 cut(s) 864
EcoICRI GAGCTC 1 cut(s) 849
EcoNI CCTNNNNNAGG 1 cut(s) 375
EcoO109I RGGNCCY 1 cut(s) 668
EcoO65I GGTNACC 1 cut(s) 864
EcoRI GAATTC 1 cut(s) 1370
EcoRII CCWGG 5 cut(s) 173, 675, 685, 715, 981
EcoT14I CCWWGG 1 cut(s) 37
EcoT38I GRGCYC 1 cut(s) 851
ErhI CCWWGG 1 cut(s) 37
FaeI CATG 6 cut(s) 215, 466, 518, 536, 741, 1271
FaqI GGGAC 1 cut(s) 1191
FatI CATG 6 cut(s) 211, 462, 514, 532, 737, 1267
FauI CCCGC 2 cut(s) 532, 822
Fnu4HI GCNGC 7 cut(s) 93, 126, 459, 546, 735, 834, 873
FokI GGATG 2 cut(s) 23, 190
FriOI GRGCYC 1 cut(s) 851
Fsp4HI GCNGC 7 cut(s) 93, 126, 459, 546, 735, 834, 873
GlaI GCGC 1 cut(s) 457
GluI GCNGC 7 cut(s) 93, 126, 459, 546, 735, 834, 873
GsaI CCCAGC 1 cut(s) 675
GsuI CTGGAG 4 cut(s) 336, 471, 624, 738
HaeII RGCGCY 1 cut(s) 459
HaeIII GGCC 4 cut(s) 141, 669, 742, 981
HapII CCGG 2 cut(s) 1010, 1424
HgaI GACGC 1 cut(s) 36
HhaI GCGC 1 cut(s) 458
Hin1II CATG 6 cut(s) 215, 466, 518, 536, 741, 1271
Hin6I GCGC 1 cut(s) 456
HinP1I GCGC 1 cut(s) 456
HincII GTYRAC 2 cut(s) 754, 988
HindII GTYRAC 2 cut(s) 754, 988
HinfI GANTC 6 cut(s) 260, 332, 466, 629, 1383, 1420
HpaII CCGG 2 cut(s) 1010, 1424
HphI GGTGA 6 cut(s) 53, 858, 1229, 1318, 1370, 1379
Hpy166II GTNNAC 3 cut(s) 754, 988, 1309
Hpy188I TCNGA 4 cut(s) 626, 696, 1186, 1206
Hpy188III TCNNGA 3 cut(s) 663, 795, 935
Hpy8I GTNNAC 3 cut(s) 754, 988, 1309
HpyAV CCTTC 8 cut(s) 297, 344, 411, 664, 914, 1133, 1167, 1253
HpyCH4III ACNGT 5 cut(s) 187, 491, 583, 619, 1443
HpyCH4IV ACGT 1 cut(s) 1391
HpyF10VI GCNNNNNNNGC 3 cut(s) 344, 455, 974
HpyF3I CTNAG 3 cut(s) 426, 821, 1185
HpySE526I ACGT 1 cut(s) 1391
Hsp92II CATG 6 cut(s) 215, 466, 518, 536, 741, 1271
HspAI GCGC 1 cut(s) 456
Kzo9I GATC 4 cut(s) 24, 353, 558, 904
LmnI GCTCC 1 cut(s) 191
Lsp1109I GCAGC 7 cut(s) 104, 137, 445, 557, 721, 845, 859
LweI GCATC 2 cut(s) 168, 826
MaeII ACGT 1 cut(s) 1391
MaeIII GTNAC 4 cut(s) 583, 864, 1168, 1330
MalI GATC 4 cut(s) 26, 355, 560, 906
MboI GATC 4 cut(s) 24, 353, 558, 904
MboII GAAGA 7 cut(s) 446, 491, 512, 568, 651, 899, 1327
MflI RGATCY 1 cut(s) 353
MhlI GDGCHC 1 cut(s) 851
MlsI TGGCCA 1 cut(s) 742
MluCI AATT 8 cut(s) 133, 574, 778, 828, 913, 1017, 1370, 1436
MluNI TGGCCA 1 cut(s) 742
MlyI GAGTC 2 cut(s) 623, 1429
MmeI TCCRAC 1 cut(s) 169
MnlI CCTC 7 cut(s) 312, 395, 401, 446, 659, 1213, 1373
Mox20I TGGCCA 1 cut(s) 742
MroXI GAANNNNTTC 1 cut(s) 945
MscI TGGCCA 1 cut(s) 742
MslI CAYNNNNRTG 3 cut(s) 822, 1272, 1304
Msp20I TGGCCA 1 cut(s) 742
MspA1I CMGCKG 4 cut(s) 128, 506, 548, 872
MspCI CTTAAG 1 cut(s) 842
MspI CCGG 2 cut(s) 1010, 1424
MspR9I CCNGG 7 cut(s) 175, 677, 687, 717, 983, 1010, 1011
Mva1269I GAATGC 1 cut(s) 726
MvaI CCWGG 5 cut(s) 175, 677, 687, 717, 983
MwoI GCNNNNNNNGC 3 cut(s) 344, 455, 974
NciI CCSGG 2 cut(s) 1010, 1011
NdeII GATC 4 cut(s) 24, 353, 558, 904
NlaIII CATG 6 cut(s) 215, 466, 518, 536, 741, 1271
NlaIV GGNNCC 3 cut(s) 355, 670, 1179
NmuCI GTSAC 3 cut(s) 864, 1168, 1330
NspI RCATGY 3 cut(s) 215, 536, 1271
OliI CACNNNNGTG 1 cut(s) 1304
PaeR7I CTCGAG 1 cut(s) 1377
PciI ACATGT 3 cut(s) 211, 532, 1267
PctI GAATGC 1 cut(s) 726
PdmI GAANNNNTTC 1 cut(s) 945
PfeI GAWTC 4 cut(s) 260, 332, 466, 1383
PflFI GACNNNGTC 1 cut(s) 491
PflMI CCANNNNNTGG 1 cut(s) 11
PfoI TCCNGGA 1 cut(s) 715
PkrI GCNGC 7 cut(s) 94, 127, 460, 547, 736, 835, 874
PleI GAGTC 2 cut(s) 623, 1428
PpsI GAGTC 2 cut(s) 623, 1428
PscI ACATGT 3 cut(s) 211, 532, 1267
PshBI ATTAAT 2 cut(s) 296, 1016
Psp124BI GAGCTC 1 cut(s) 851
Psp6I CCWGG 5 cut(s) 173, 675, 685, 715, 981
PspEI GGTNACC 1 cut(s) 864
PspFI CCCAGC 1 cut(s) 671
PspGI CCWGG 5 cut(s) 173, 675, 685, 715, 981
PspN4I GGNNCC 3 cut(s) 355, 670, 1179
PspPI GGNCC 4 cut(s) 140, 668, 713, 1178
PspXI VCTCGAGB 1 cut(s) 1377
PstI CTGCAG 1 cut(s) 958
PsuI RGATCY 1 cut(s) 353
PsyI GACNNNGTC 1 cut(s) 491
PvuII CAGCTG 4 cut(s) 128, 506, 548, 872
RsaI GTAC 1 cut(s) 1266
RsaNI GTAC 1 cut(s) 1265
RseI CAYNNNNRTG 3 cut(s) 822, 1272, 1304
SacI GAGCTC 1 cut(s) 851
SatI GCNGC 7 cut(s) 93, 126, 459, 546, 735, 834, 873
Sau3AI GATC 4 cut(s) 24, 353, 558, 904
Sau96I GGNCC 4 cut(s) 140, 668, 713, 1178
SchI GAGTC 2 cut(s) 623, 1429
ScrFI CCNGG 7 cut(s) 175, 677, 687, 717, 983, 1010, 1011
SduI GDGCHC 1 cut(s) 851
SfaNI GCATC 2 cut(s) 168, 826
SfcI CTRYAG 1 cut(s) 954
Sfr274I CTCGAG 1 cut(s) 1377
SinI GGWCC 2 cut(s) 713, 1178
SlaI CTCGAG 1 cut(s) 1377
SmaI CCCGGG 1 cut(s) 1011
SmiMI CAYNNNNRTG 3 cut(s) 822, 1272, 1304
SmlI CTYRAG 2 cut(s) 842, 1377
SmoI CTYRAG 2 cut(s) 842, 1377
Sse9I AATT 8 cut(s) 133, 574, 778, 828, 913, 1017, 1370, 1436
SsiI CCGC 2 cut(s) 525, 815
SspI AATATT 1 cut(s) 111
SstI GAGCTC 1 cut(s) 851
StyD4I CCNGG 7 cut(s) 173, 675, 685, 715, 981, 1008, 1009
StyI CCWWGG 1 cut(s) 37
TaaI ACNGT 5 cut(s) 187, 491, 583, 619, 1443
TaiI ACGT 1 cut(s) 1394
TaqI TCGA 3 cut(s) 936, 1127, 1378
TasI AATT 8 cut(s) 133, 574, 778, 828, 913, 1017, 1370, 1436
TatI WGTACW 1 cut(s) 1264
TfiI GAWTC 4 cut(s) 260, 332, 466, 1383
TscAI CASTG 7 cut(s) 79, 283, 648, 829, 976, 1179, 1335
TseFI GTSAC 3 cut(s) 864, 1168, 1330
TseI GCWGC 7 cut(s) 92, 125, 458, 545, 734, 833, 872
Tsp45I GTSAC 3 cut(s) 864, 1168, 1330
TspDTI ATGAA 6 cut(s) 180, 492, 503, 954, 1095, 1218
TspMI CCCGGG 1 cut(s) 1009
TspRI CASTG 7 cut(s) 79, 283, 648, 829, 976, 1179, 1335
Tth111I GACNNNGTC 1 cut(s) 491
Van91I CCANNNNNTGG 1 cut(s) 11
Vha464I CTTAAG 1 cut(s) 842
VpaK11BI GGWCC 2 cut(s) 713, 1178
VspI ATTAAT 2 cut(s) 296, 1016
XagI CCTNNNNNAGG 1 cut(s) 375
XapI RAATTY 4 cut(s) 133, 574, 913, 1370
XceI RCATGY 3 cut(s) 215, 536, 1271
XhoI CTCGAG 1 cut(s) 1377
XmaI CCCGGG 1 cut(s) 1009
XmnI GAANNNNTTC 1 cut(s) 945
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.