Rh7DG186400

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000302
Physical Location & Seq
Reverse (-)
17310083 .. 17310721
639 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG186400.1

Sequence Viewer

Length: 639 bp
ATGGCTGTGATTCCATTTCTTTTTCTTGTTTTCATGCTTAGCTTTTCTCATGGGATTGCTCAGGAAACCGAGCTTTCTCATGTCCCTACGAAAACGAGCAATTTACAGACATACATTGTCCATGTAATGCAACCAGAGGGTAAAGTTTTTGCCCAAAGAGAAGACCTGGCGAGCTGGCACAAATCTTTTCTGCCATCTATGACGGCAAGCTCTGATGAGCAAACACGCTTGCTTTACTCCTACAAAACTGTGATCAGTGGTTTTTCAGCAAGGCTAACTCAAGAGGAAGTGAAAGCAATGGAACAGATGAATGGTTTTGTGGCGGCACATCCTGAACGAGTGTTTCGTCGGAAAACCACACACACTCCCAACTTCTTGGGGCTGAACCAACAAGCAGGAATTTGGAAAGACTCAAACTTTGGGAAGGGAGTGATCATTGGAGTACTGGATGGCGGAGTATTCCCCAGCCACCCTTCATTCAGTGGTGCAGGAATTCCACCTCCACCTGCTAAATGGAAAGGGAGGTGTGACTTCAATGTGTCAGAATGCAATAATAAATTAATTGGAGCACAGTCGTTCAATCTTGCAGCCATGGTTCTCTCAAAAAAAAAAAAAAAAATCTTGCAGCCATGGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

212

Amino Acids

23.62

Weight (kDa)

9.6

Isoelectric Point (pI)

40.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 37 - 115 2.7e-14 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 514
Acc36I ACCTGC 1 cut(s) 514
AciI CCGC 2 cut(s) 323, 453
AcsI RAATTY 2 cut(s) 399, 492
AfaI GTAC 1 cut(s) 444
AgsI TTSAA 2 cut(s) 535, 580
AjnI CCWGG 1 cut(s) 165
AluBI AGCT 4 cut(s) 42, 73, 174, 210
AluI AGCT 4 cut(s) 42, 73, 174, 210
Alw21I GWGCWC 1 cut(s) 571
ApeKI GCWGC 2 cut(s) 587, 625
ApoI RAATTY 2 cut(s) 399, 492
ArsI GACNNNNNNTTYG 2 cut(s) 401, 433
AseI ATTAAT 1 cut(s) 560
BbsI GAAGAC 1 cut(s) 168
Bbv12I GWGCWC 1 cut(s) 571
BbvI GCAGC 1 cut(s) 599
BccI CCATC 2 cut(s) 202, 443
BceAI ACGGC 1 cut(s) 219
BciT130I CCWGG 1 cut(s) 167
BclI TGATCA 2 cut(s) 252, 432
BfuAI ACCTGC 1 cut(s) 514
BisI GCNGC 3 cut(s) 324, 588, 626
BlpI GCTNAGC 1 cut(s) 38
BlsI GCNGC 3 cut(s) 325, 589, 627
BmcAI AGTACT 1 cut(s) 444
Bme1390I CCNGG 1 cut(s) 167
BmrFI CCNGG 1 cut(s) 167
BpiI GAAGAC 1 cut(s) 168
Bpu10I CCTNAGC 1 cut(s) 60
Bpu1102I GCTNAGC 1 cut(s) 38
BpuEI CTTGAG 1 cut(s) 264
BsaJI CCNNGG 2 cut(s) 591, 629
BsaXI ACNNNNNCTCC 2 cut(s) 349, 379
Bse1I ACTGG 1 cut(s) 450
Bse3DI GCAATG 1 cut(s) 303
BseBI CCWGG 1 cut(s) 167
BseDI CCNNGG 2 cut(s) 591, 629
BseGI GGATG 2 cut(s) 328, 454
BseMI GCAATG 1 cut(s) 303
BseMII CTCAG 1 cut(s) 74
BseNI ACTGG 1 cut(s) 450
BseXI GCAGC 1 cut(s) 599
BseYI CCCAGC 1 cut(s) 464
BsgI GTGCAG 1 cut(s) 507
BsiHKAI GWGCWC 1 cut(s) 571
BslFI GGGAC 1 cut(s) 68
BsmFI GGGAC 1 cut(s) 68
BsmI GAATGC 1 cut(s) 551
Bsp1286I GDGCHC 1 cut(s) 571
Bsp143I GATC 2 cut(s) 252, 432
Bsp1720I GCTNAGC 1 cut(s) 38
Bsp19I CCATGG 2 cut(s) 591, 629
BspACI CCGC 2 cut(s) 323, 453
BspCNI CTCAG 1 cut(s) 73
BspMI ACCTGC 1 cut(s) 514
BsrDI GCAATG 1 cut(s) 303
BsrI ACTGG 1 cut(s) 450
BssECI CCNNGG 2 cut(s) 591, 629
BssMI GATC 2 cut(s) 252, 432
BssT1I CCWWGG 2 cut(s) 591, 629
Bst2UI CCWGG 1 cut(s) 167
Bst4CI ACNGT 2 cut(s) 250, 573
BstC8I GCNNGC 4 cut(s) 172, 176, 208, 230
BstDEI CTNAG 2 cut(s) 38, 60
BstDSI CCRYGG 2 cut(s) 591, 629
BstF5I GGATG 2 cut(s) 328, 454
BstKTI GATC 2 cut(s) 255, 435
BstMBI GATC 2 cut(s) 252, 432
BstMWI GCNNNNNNNGC 1 cut(s) 631
BstNI CCWGG 1 cut(s) 167
BstSCI CCNGG 1 cut(s) 165
BstV1I GCAGC 1 cut(s) 599
BstV2I GAAGAC 1 cut(s) 168
BstXI CCANNNNNNTGG 1 cut(s) 376
BtgI CCRYGG 2 cut(s) 591, 629
BtsCI GGATG 2 cut(s) 328, 454
BtsIMutI CAGTG 2 cut(s) 262, 487
BveI ACCTGC 1 cut(s) 514
Cac8I GCNNGC 4 cut(s) 172, 176, 208, 230
Csp6I GTAC 1 cut(s) 443
CviAII CATG 6 cut(s) 34, 50, 80, 122, 592, 630
CviQI GTAC 1 cut(s) 443
DdeI CTNAG 2 cut(s) 38, 60
DpnI GATC 2 cut(s) 254, 434
DpnII GATC 2 cut(s) 252, 432
EciI GGCGGA 1 cut(s) 468
Eco130I CCWWGG 2 cut(s) 591, 629
EcoRI GAATTC 1 cut(s) 492
EcoRII CCWGG 1 cut(s) 165
EcoT14I CCWWGG 2 cut(s) 591, 629
ErhI CCWWGG 2 cut(s) 591, 629
FaeI CATG 6 cut(s) 37, 53, 83, 125, 595, 633
FaiI YATR 8 cut(s) 35, 51, 81, 112, 123, 200, 593, 631
FaqI GGGAC 1 cut(s) 68
FatI CATG 6 cut(s) 33, 49, 79, 121, 591, 629
FbaI TGATCA 2 cut(s) 252, 432
Fnu4HI GCNGC 3 cut(s) 324, 588, 626
FokI GGATG 2 cut(s) 315, 461
Fsp4HI GCNGC 3 cut(s) 324, 588, 626
GluI GCNGC 3 cut(s) 324, 588, 626
GsaI CCCAGC 1 cut(s) 468
Hin1II CATG 6 cut(s) 37, 53, 83, 125, 595, 633
HinfI GANTC 2 cut(s) 10, 410
Hpy188I TCNGA 3 cut(s) 214, 351, 544
Hpy188III TCNNGA 3 cut(s) 62, 281, 332
Hpy99I CGWCG 1 cut(s) 351
HpyAV CCTTC 2 cut(s) 418, 483
HpyCH4III ACNGT 2 cut(s) 250, 573
HpyCH4V TGCA 5 cut(s) 130, 488, 549, 587, 625
HpyF10VI GCNNNNNNNGC 1 cut(s) 631
HpyF3I CTNAG 2 cut(s) 38, 60
Hsp92II CATG 6 cut(s) 37, 53, 83, 125, 595, 633
Ksp22I TGATCA 2 cut(s) 252, 432
Kzo9I GATC 2 cut(s) 252, 432
LmnI GCTCC 1 cut(s) 566
Lsp1109I GCAGC 1 cut(s) 599
MaeIII GTNAC 1 cut(s) 527
MalI GATC 2 cut(s) 254, 434
MboI GATC 2 cut(s) 252, 432
MboII GAAGA 1 cut(s) 173
MhlI GDGCHC 1 cut(s) 571
MluCI AATT 5 cut(s) 100, 399, 492, 557, 561
MlyI GAGTC 1 cut(s) 404
MmeI TCCRAC 1 cut(s) 329
MnlI CCTC 4 cut(s) 130, 277, 510, 516
MseI TTAA 1 cut(s) 560
MspR9I CCNGG 1 cut(s) 167
Mva1269I GAATGC 1 cut(s) 551
MvaI CCWGG 1 cut(s) 167
MwoI GCNNNNNNNGC 1 cut(s) 631
NcoI CCATGG 2 cut(s) 591, 629
NdeII GATC 2 cut(s) 252, 432
NlaIII CATG 6 cut(s) 37, 53, 83, 125, 595, 633
NmuCI GTSAC 1 cut(s) 527
PaqCI CACCTGC 1 cut(s) 514
PcsI WCGNNNNNNNCGW 1 cut(s) 343
PctI GAATGC 1 cut(s) 551
PfeI GAWTC 1 cut(s) 10
PkrI GCNGC 3 cut(s) 325, 589, 627
PleI GAGTC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 404
PshBI ATTAAT 1 cut(s) 560
Psp6I CCWGG 1 cut(s) 165
PspFI CCCAGC 1 cut(s) 464
PspGI CCWGG 1 cut(s) 165
RsaI GTAC 1 cut(s) 444
RsaNI GTAC 1 cut(s) 443
SaqAI TTAA 1 cut(s) 560
SatI GCNGC 3 cut(s) 324, 588, 626
Sau3AI GATC 2 cut(s) 252, 432
ScaI AGTACT 1 cut(s) 444
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 1 cut(s) 167
SduI GDGCHC 1 cut(s) 571
SetI ASST 8 cut(s) 44, 75, 168, 176, 212, 502, 508, 527
SmlI CTYRAG 1 cut(s) 279
SmoI CTYRAG 1 cut(s) 279
Sse9I AATT 5 cut(s) 100, 399, 492, 557, 561
SsiI CCGC 2 cut(s) 323, 453
StyD4I CCNGG 1 cut(s) 165
StyI CCWWGG 2 cut(s) 591, 629
TaaI ACNGT 2 cut(s) 250, 573
TasI AATT 5 cut(s) 100, 399, 492, 557, 561
TatI WGTACW 1 cut(s) 442
TauI GCSGC 1 cut(s) 326
TfiI GAWTC 1 cut(s) 10
Tru1I TTAA 1 cut(s) 560
Tru9I TTAA 1 cut(s) 560
TscAI CASTG 2 cut(s) 262, 487
TseFI GTSAC 1 cut(s) 527
TseI GCWGC 2 cut(s) 587, 625
Tsp45I GTSAC 1 cut(s) 527
TspDTI ATGAA 3 cut(s) 22, 323, 465
TspRI CASTG 2 cut(s) 262, 487
VspI ATTAAT 1 cut(s) 560
XapI RAATTY 2 cut(s) 399, 492
XcmI CCANNNNNNNNNTGG 1 cut(s) 510
ZrmI AGTACT 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.