Rorug07G0058200

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000302
Physical Location & Seq
Reverse (-)
4183699 .. 4189134
5436 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0058200.1

Sequence Viewer

Length: 1716 bp
ATGGAGCTGCCACAGCCCCGTCCGTTTGGAGGAGGCCAAGGAGGAAGAAAACCAACGCATGACTTTCTGTCGTTGTACACTAGTCATTCAAATGCCCAACAAGATCCAATACCACCTTCTCAAGGAGGTTATCTTGAAACTCATGATTTTCTACAACCACTTGAACGAATGGGAAAGACCTGTGCCAAAGAAGAAACCTCGGTTGAGATCATATCACCAAGTAGAAGTAGTGTTGAAAAGCCGCCATGTGTGGCGCAACGGCCAACTTCTGTGGAGCACATTCTTCCCGGTGGGATTGGGACTTACAGTATAAGCCACATTTCGTATTTTAATCAAAGGGTTCCAAAACCAGAGGAAAACGTATTCCCGGTTGCTCCTCAAGCAAGTAGTACCCATGAAAGGAATGATGAAAACTCAAACTGCAGTTCTTATGCCGGAAGTGGTTTCACTTTGTGGGACGAATCTGCAGCAATGAAGGGAAAGACAGGGAAGGAGAATGTGGTGGAAAGACCAGGTTTAAGAGGGCAATGGACGTCGTCAGAGAGGCCATCACAGTCATCTTCCACTAACAACCGCAACTGCTTCAGCTCTCAGTCATCTGGGCAGAAGAACCTGAGCTTCATGGAGATGATGAAGTCCGCTGCCAAGGGTTGCACCCAGGAAGAAGAACTTGACGATGAGGAAGAGTTTGCTCTGAAAAAGGAGATGTCCACTACTACTACCACTGCCACTACCCCCTACAAGGGAGAATTACGGGTTAAGGTTGATGTTAAGAGCTCTGATCAGAAAGCTAACACGCCACGATCCAAACATTCTGCCACTGAACAGCGAAGGAGGAGTAAAATAAATGACAGATTCCAAATGTTGAGAGAGCTCATTCCTCATAGTGATCAAAAGAGAGACAAGGCATCATTTCTATTAGAGGTTATCGAATACATTCAGTATTTACAGGAAAAAGTAACCAAGTATGAGGGTCCATACCAAGGTTGGAACCATGATCCAGCAAAATTGATGCCTTGGGGAAACAACAAGCTTGGGGAAAGTTACTGTGATCAATCTCGAGGTATGAATGGTGGATCTGCTCCTGCATTACTGTTTGCTGCTGCAAAATTTGAGGAGAAAACTACTTCAGTGTCTCCTACTATTCCTGGAAGTGCTCAGAACCTGGTAGAATCTGATACGAGTACTGAAACTACATTCAGAGGTAAGGATTACCCCAATGGAATAACAAACAAGGCAAATCCCTTTCCTCTGTCAATGCAGTCGAACTTCTCGACACCTGTCAGAAGTGGTAGTGCAGCACCTCATCTTCCACCTAGATTGCCATCTGATGCAGAGAACACATCATCTCATCCTCGCTCCATGTTATTCCCGACAAAATCATGTACTGCTGATGCTGGTGCCATTAGTGATAAGCTAAAAGAACATGAACTTACAATTGAAGGTGGAAGAATTAACATCTCAAGTGCGTACTCTCAAGGGTTATTAAATACTCTTACACAAGCCCTGCAGAATTCTGGTGTGGATTTGTCACAGGCCAGCATCTCGGTGCAAATCGAGCTTGGGAAGCAAGTGTGTAGTAGAACGGCTATACCCAAATCCATTGTCAAGGAAAATGAGATTCCCTCTATGACTCCAGGGAAAAAGCACCCTAGAATTTCAGTCGGTGAAGACTCAGAACAAGCCCAAAAGAAGCTCAGGACAGGCAGACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

571

Amino Acids

62.74

Weight (kDa)

8.0

Isoelectric Point (pI)

52.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 268 - 317 2.1e-16 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 536
AccB1I GGYRCC 1 cut(s) 1400
AciI CCGC 3 cut(s) 242, 574, 639
AclWI GGATC 4 cut(s) 98, 798, 992, 1084
AcoI YGGCCR 1 cut(s) 260
AcsI RAATTY 3 cut(s) 1109, 1513, 1656
AcuI CTGAAG 2 cut(s) 568, 1113
AcyI GRCGYC 1 cut(s) 533
AdeI CACNNNGTG 1 cut(s) 453
AfaI GTAC 5 cut(s) 77, 391, 1186, 1387, 1472
AfiI CCNNNNNNNGG 6 cut(s) 29, 122, 399, 742, 743, 983
AgsI TTSAA 5 cut(s) 90, 137, 164, 236, 1442
AhdI GACNNNNNGTC 1 cut(s) 67
AhlI ACTAGT 1 cut(s) 80
AjnI CCWGG 5 cut(s) 511, 657, 1147, 1164, 1636
AjuI GAANNNNNNNTTGG 2 cut(s) 100, 132
Alw21I GWGCWC 4 cut(s) 279, 779, 876, 1159
Alw26I GTCTC 2 cut(s) 894, 1140
AlwI GGATC 4 cut(s) 98, 798, 992, 1084
AlwNI CAGNNNCTG 1 cut(s) 1165
Ama87I CYCGRG 1 cut(s) 1059
AoxI GGCC 4 cut(s) 34, 260, 545, 1536
ApeKI GCWGC 6 cut(s) 7, 467, 641, 1100, 1103, 1298
ApoI RAATTY 3 cut(s) 1109, 1513, 1656
ArsI GACNNNNNNTTYG 2 cut(s) 1590, 1622
Asp700I GAANNNNTTC 1 cut(s) 936
AspLEI GCGC 1 cut(s) 256
AspS9I GGNCC 1 cut(s) 974
AsuC2I CCSGG 2 cut(s) 288, 368
AsuHPI GGTGA 2 cut(s) 207, 1679
AvaI CYCGRG 1 cut(s) 1059
AvaII GGWCC 1 cut(s) 974
BanI GGYRCC 1 cut(s) 1400
BanII GRGCYC 2 cut(s) 779, 876
BbsI GAAGAC 1 cut(s) 1677
Bbv12I GWGCWC 4 cut(s) 279, 779, 876, 1159
BbvI GCAGC 5 cut(s) 479, 628, 1087, 1090, 1310
BccI CCATC 2 cut(s) 556, 1333
BceAI ACGGC 2 cut(s) 275, 1602
BciT130I CCWGG 5 cut(s) 513, 659, 1149, 1166, 1638
BclI TGATCA 3 cut(s) 781, 889, 1051
BcnI CCSGG 2 cut(s) 288, 368
BcoDI GTCTC 2 cut(s) 894, 1140
BcuI ACTAGT 1 cut(s) 80
BfaI CTAG 3 cut(s) 81, 1317, 1653
BfmI CTRYAG 3 cut(s) 421, 465, 1508
BisI GCNGC 7 cut(s) 8, 242, 468, 642, 1101, 1104, 1299
BlsI GCNGC 7 cut(s) 9, 243, 469, 643, 1102, 1105, 1300
BmcAI AGTACT 1 cut(s) 1186
Bme1390I CCNGG 7 cut(s) 288, 368, 513, 659, 1149, 1166, 1638
Bme18I GGWCC 1 cut(s) 974
BmeRI GACNNNNNGTC 1 cut(s) 67
BmeT110I CYCGRG 1 cut(s) 1059
BmgT120I GGNCC 1 cut(s) 974
BmiI GGNNCC 4 cut(s) 342, 975, 992, 1402
BmrFI CCNGG 7 cut(s) 288, 368, 513, 659, 1149, 1166, 1638
BmsI GCATC 5 cut(s) 917, 1002, 1321, 1384, 1551
BoxI GACNNNNGTC 1 cut(s) 1280
BpiI GAAGAC 1 cut(s) 1677
BplI GAGNNNNNCTC 2 cut(s) 1610, 1642
BpmI CTGGAG 1 cut(s) 1620
Bpu10I CCTNAGC 2 cut(s) 614, 1697
BpuEI CTTGAG 4 cut(s) 105, 363, 1447, 1461
BpuMI CCSGG 2 cut(s) 288, 368
BsaBI GATNNNNATC 1 cut(s) 1324
BsaHI GRCGYC 1 cut(s) 533
BsaJI CCNNGG 7 cut(s) 37, 198, 645, 657, 982, 1016, 1637
Bsc4I CCNNNNNNNGG 6 cut(s) 29, 122, 399, 742, 743, 983
Bse3DI GCAATG 2 cut(s) 477, 533
Bse8I GATNNNNATC 1 cut(s) 1324
BseBI CCWGG 5 cut(s) 513, 659, 1149, 1166, 1638
BseDI CCNNGG 7 cut(s) 37, 198, 645, 657, 982, 1016, 1637
BseGI GGATG 1 cut(s) 1351
BseJI GATNNNNATC 1 cut(s) 1324
BseLI CCNNNNNNNGG 6 cut(s) 29, 122, 399, 742, 743, 983
BseMI GCAATG 2 cut(s) 477, 533
BseMII CTCAG 5 cut(s) 605, 605, 1172, 1689, 1711
BseRI GAGGAG 4 cut(s) 45, 366, 850, 1130
BseXI GCAGC 5 cut(s) 479, 628, 1087, 1090, 1310
BsgI GTGCAG 1 cut(s) 1317
BshFI GGCC 4 cut(s) 36, 262, 547, 1538
BshNI GGYRCC 1 cut(s) 1400
BsiHKAI GWGCWC 4 cut(s) 279, 779, 876, 1159
BsiHKCI CYCGRG 1 cut(s) 1059
BsiSI CCGG 3 cut(s) 288, 368, 435
BslFI GGGAC 2 cut(s) 313, 470
BslI CCNNNNNNNGG 6 cut(s) 29, 122, 399, 742, 743, 983
BsmAI GTCTC 2 cut(s) 894, 1140
BsmFI GGGAC 2 cut(s) 313, 470
BsnI GGCC 4 cut(s) 36, 262, 547, 1538
BsoBI CYCGRG 1 cut(s) 1059
Bsp1286I GDGCHC 4 cut(s) 279, 779, 876, 1159
Bsp1407I TGTACA 1 cut(s) 75
Bsp143I GATC 8 cut(s) 103, 207, 781, 803, 889, 997, 1051, 1076
BspACI CCGC 3 cut(s) 242, 574, 639
BspANI GGCC 4 cut(s) 36, 262, 547, 1538
BspCNI CTCAG 5 cut(s) 604, 606, 1171, 1688, 1710
BspHI TCATGA 1 cut(s) 142
BspLI GGNNCC 4 cut(s) 342, 975, 992, 1402
BspMAI CTGCAG 3 cut(s) 425, 469, 1512
BspPI GGATC 4 cut(s) 98, 798, 992, 1084
BspT107I GGYRCC 1 cut(s) 1400
BsrDI GCAATG 2 cut(s) 477, 533
BsrGI TGTACA 1 cut(s) 75
BssECI CCNNGG 7 cut(s) 37, 198, 645, 657, 982, 1016, 1637
BssMI GATC 8 cut(s) 103, 207, 781, 803, 889, 997, 1051, 1076
BssNI GRCGYC 1 cut(s) 533
BssT1I CCWWGG 4 cut(s) 37, 645, 982, 1016
Bst2UI CCWGG 5 cut(s) 513, 659, 1149, 1166, 1638
Bst4CI ACNGT 4 cut(s) 308, 555, 1049, 1095
Bst6I CTCTTC 1 cut(s) 678
BstACI GRCGYC 1 cut(s) 533
BstAUI TGTACA 1 cut(s) 75
BstC8I GCNNGC 1 cut(s) 1540
BstDEI CTNAG 5 cut(s) 591, 614, 1158, 1675, 1697
BstENI CCTNNNNNAGG 1 cut(s) 120
BstF5I GGATG 1 cut(s) 1351
BstHHI GCGC 1 cut(s) 256
BstKTI GATC 8 cut(s) 106, 210, 784, 806, 892, 1000, 1054, 1079
BstMAI GTCTC 2 cut(s) 894, 1140
BstMBI GATC 8 cut(s) 103, 207, 781, 803, 889, 997, 1051, 1076
BstMWI GCNNNNNNNGC 4 cut(s) 13, 380, 1558, 1567
BstNI CCWGG 5 cut(s) 513, 659, 1149, 1166, 1638
BstPAI GACNNNNGTC 1 cut(s) 1280
BstSCI CCNGG 7 cut(s) 286, 366, 511, 657, 1147, 1164, 1636
BstSFI CTRYAG 3 cut(s) 421, 465, 1508
BstV1I GCAGC 5 cut(s) 479, 628, 1087, 1090, 1310
BstV2I GAAGAC 1 cut(s) 1677
BstX2I RGATCY 2 cut(s) 103, 1076
BstYI RGATCY 2 cut(s) 103, 1076
BsuRI GGCC 4 cut(s) 36, 262, 547, 1538
BtsCI GGATG 1 cut(s) 1351
BtsI GCAGTG 1 cut(s) 723
BtsIMutI CAGTG 3 cut(s) 723, 819, 1137
Cac8I GCNNGC 1 cut(s) 1540
CaiI CAGNNNCTG 1 cut(s) 1165
CciI TCATGA 1 cut(s) 142
CfoI GCGC 1 cut(s) 256
Cfr13I GGNCC 1 cut(s) 974
CsiI ACCWGGT 2 cut(s) 511, 1164
Csp6I GTAC 5 cut(s) 76, 390, 1185, 1386, 1471
CspCI CAANNNNNGTGG 4 cut(s) 252, 287, 1302, 1337
CviAII CATG 9 cut(s) 59, 143, 246, 395, 622, 995, 1363, 1383, 1427
CviQI GTAC 5 cut(s) 76, 390, 1185, 1386, 1471
DdeI CTNAG 5 cut(s) 591, 614, 1158, 1675, 1697
DpnI GATC 8 cut(s) 105, 209, 783, 805, 891, 999, 1053, 1078
DpnII GATC 8 cut(s) 103, 207, 781, 803, 889, 997, 1051, 1076
DraIII CACNNNGTG 1 cut(s) 453
DriI GACNNNNNGTC 1 cut(s) 67
EaeI YGGCCR 1 cut(s) 260
Eam1104I CTCTTC 1 cut(s) 678
Eam1105I GACNNNNNGTC 1 cut(s) 67
EarI CTCTTC 1 cut(s) 678
Ecl136II GAGCTC 2 cut(s) 777, 874
Eco130I CCWWGG 4 cut(s) 37, 645, 982, 1016
Eco24I GRGCYC 2 cut(s) 779, 876
Eco47I GGWCC 1 cut(s) 974
Eco53kI GAGCTC 2 cut(s) 777, 874
Eco57I CTGAAG 2 cut(s) 568, 1113
Eco88I CYCGRG 1 cut(s) 1059
EcoICRI GAGCTC 2 cut(s) 777, 874
EcoNI CCTNNNNNAGG 1 cut(s) 120
EcoRI GAATTC 1 cut(s) 1513
EcoRII CCWGG 5 cut(s) 511, 657, 1147, 1164, 1636
EcoT14I CCWWGG 4 cut(s) 37, 645, 982, 1016
EcoT38I GRGCYC 2 cut(s) 779, 876
ErhI CCWWGG 4 cut(s) 37, 645, 982, 1016
FaeI CATG 9 cut(s) 62, 146, 249, 398, 625, 998, 1366, 1386, 1430
FalI AAGNNNNNCTT 2 cut(s) 654, 686
FaqI GGGAC 2 cut(s) 313, 470
FatI CATG 9 cut(s) 58, 142, 245, 394, 621, 994, 1362, 1382, 1426
FbaI TGATCA 3 cut(s) 781, 889, 1051
Fnu4HI GCNGC 7 cut(s) 8, 242, 468, 642, 1101, 1104, 1299
FokI GGATG 1 cut(s) 1338
FriOI GRGCYC 2 cut(s) 779, 876
Fsp4HI GCNGC 7 cut(s) 8, 242, 468, 642, 1101, 1104, 1299
FspBI CTAG 3 cut(s) 81, 1317, 1653
GlaI GCGC 1 cut(s) 255
GluI GCNGC 7 cut(s) 8, 242, 468, 642, 1101, 1104, 1299
GsuI CTGGAG 1 cut(s) 1620
HaeIII GGCC 4 cut(s) 36, 262, 547, 1538
HapII CCGG 3 cut(s) 288, 368, 435
HhaI GCGC 1 cut(s) 256
Hin1I GRCGYC 1 cut(s) 533
Hin1II CATG 9 cut(s) 62, 146, 249, 398, 625, 998, 1366, 1386, 1430
Hin6I GCGC 1 cut(s) 254
HinP1I GCGC 1 cut(s) 254
HindIII AAGCTT 1 cut(s) 1031
HinfI GANTC 6 cut(s) 461, 855, 1172, 1621, 1633, 1673
HpaII CCGG 3 cut(s) 288, 368, 435
HphI GGTGA 2 cut(s) 207, 1679
Hpy166II GTNNAC 2 cut(s) 78, 711
Hpy188III TCNNGA 6 cut(s) 134, 143, 1059, 1273, 1372, 1699
Hpy8I GTNNAC 2 cut(s) 78, 711
Hpy99I CGWCG 1 cut(s) 538
HpyAV CCTTC 5 cut(s) 126, 469, 484, 825, 1436
HpyCH4III ACNGT 4 cut(s) 308, 555, 1049, 1095
HpyCH4IV ACGT 2 cut(s) 360, 533
HpyF10VI GCNNNNNNNGC 4 cut(s) 13, 380, 1558, 1567
HpyF3I CTNAG 5 cut(s) 591, 614, 1158, 1675, 1697
HpySE526I ACGT 2 cut(s) 360, 533
Hsp92I GRCGYC 1 cut(s) 533
Hsp92II CATG 9 cut(s) 62, 146, 249, 398, 625, 998, 1366, 1386, 1430
HspAI GCGC 1 cut(s) 254
Ksp22I TGATCA 3 cut(s) 781, 889, 1051
Kzo9I GATC 8 cut(s) 103, 207, 781, 803, 889, 997, 1051, 1076
LmnI GCTCC 5 cut(s) 4, 274, 379, 1087, 1364
Lsp1109I GCAGC 5 cut(s) 479, 628, 1087, 1090, 1310
LweI GCATC 5 cut(s) 917, 1002, 1321, 1384, 1551
MabI ACCWGGT 2 cut(s) 511, 1164
MaeI CTAG 3 cut(s) 81, 1317, 1653
MaeII ACGT 2 cut(s) 360, 533
MaeIII GTNAC 3 cut(s) 958, 1043, 1530
MalI GATC 8 cut(s) 105, 209, 783, 805, 891, 999, 1053, 1078
MboI GATC 8 cut(s) 103, 207, 781, 803, 889, 997, 1051, 1076
MfeI CAATTG 1 cut(s) 1437
MflI RGATCY 2 cut(s) 103, 1076
MhlI GDGCHC 4 cut(s) 279, 779, 876, 1159
MluCI AATT 7 cut(s) 749, 1007, 1109, 1437, 1452, 1513, 1656
MlyI GAGTC 2 cut(s) 1627, 1667
MmeI TCCRAC 1 cut(s) 968
MroXI GAANNNNTTC 1 cut(s) 936
MseI TTAA 6 cut(s) 330, 518, 759, 771, 1455, 1487
MslI CAYNNNNRTG 3 cut(s) 90, 626, 1547
MspA1I CMGCKG 1 cut(s) 641
MspI CCGG 3 cut(s) 288, 368, 435
MspR9I CCNGG 7 cut(s) 288, 368, 513, 659, 1149, 1166, 1638
MunI CAATTG 1 cut(s) 1437
MvaI CCWGG 5 cut(s) 513, 659, 1149, 1166, 1638
MwoI GCNNNNNNNGC 4 cut(s) 13, 380, 1558, 1567
NciI CCSGG 2 cut(s) 288, 368
NdeII GATC 8 cut(s) 103, 207, 781, 803, 889, 997, 1051, 1076
NlaIII CATG 9 cut(s) 62, 146, 249, 398, 625, 998, 1366, 1386, 1430
NlaIV GGNNCC 4 cut(s) 342, 975, 992, 1402
NmuCI GTSAC 1 cut(s) 1530
PaeR7I CTCGAG 1 cut(s) 1059
PagI TCATGA 1 cut(s) 142
PcsI WCGNNNNNNNCGW 1 cut(s) 1271
PdmI GAANNNNTTC 1 cut(s) 936
PfeI GAWTC 4 cut(s) 461, 855, 1172, 1621
PflFI GACNNNGTC 1 cut(s) 535
PfoI TCCNGGA 1 cut(s) 1147
PkrI GCNGC 7 cut(s) 9, 243, 469, 643, 1102, 1105, 1300
PleI GAGTC 2 cut(s) 1627, 1667
PpsI GAGTC 2 cut(s) 1627, 1667
PshAI GACNNNNGTC 1 cut(s) 1280
Psp124BI GAGCTC 2 cut(s) 779, 876
Psp6I CCWGG 5 cut(s) 511, 657, 1147, 1164, 1636
PspGI CCWGG 5 cut(s) 511, 657, 1147, 1164, 1636
PspN4I GGNNCC 4 cut(s) 342, 975, 992, 1402
PspPI GGNCC 1 cut(s) 974
PstI CTGCAG 3 cut(s) 425, 469, 1512
PstNI CAGNNNCTG 1 cut(s) 1165
PsuI RGATCY 2 cut(s) 103, 1076
PsyI GACNNNGTC 1 cut(s) 535
RsaI GTAC 5 cut(s) 77, 391, 1186, 1387, 1472
RsaNI GTAC 5 cut(s) 76, 390, 1185, 1386, 1471
RseI CAYNNNNRTG 3 cut(s) 90, 626, 1547
SacI GAGCTC 2 cut(s) 779, 876
SaqAI TTAA 6 cut(s) 330, 518, 759, 771, 1455, 1487
SatI GCNGC 7 cut(s) 8, 242, 468, 642, 1101, 1104, 1299
Sau3AI GATC 8 cut(s) 103, 207, 781, 803, 889, 997, 1051, 1076
Sau96I GGNCC 1 cut(s) 974
ScaI AGTACT 1 cut(s) 1186
SchI GAGTC 2 cut(s) 1627, 1667
ScrFI CCNGG 7 cut(s) 288, 368, 513, 659, 1149, 1166, 1638
SduI GDGCHC 4 cut(s) 279, 779, 876, 1159
SexAI ACCWGGT 2 cut(s) 511, 1164
SfaNI GCATC 5 cut(s) 917, 1002, 1321, 1384, 1551
SfcI CTRYAG 3 cut(s) 421, 465, 1508
Sfr274I CTCGAG 1 cut(s) 1059
SinI GGWCC 1 cut(s) 974
SlaI CTCGAG 1 cut(s) 1059
SmiMI CAYNNNNRTG 3 cut(s) 90, 626, 1547
SmlI CTYRAG 5 cut(s) 120, 378, 1059, 1462, 1476
SmoI CTYRAG 5 cut(s) 120, 378, 1059, 1462, 1476
SpeI ACTAGT 1 cut(s) 80
Sse9I AATT 7 cut(s) 749, 1007, 1109, 1437, 1452, 1513, 1656
SsiI CCGC 3 cut(s) 242, 574, 639
SspMI CTAG 3 cut(s) 81, 1317, 1653
SstI GAGCTC 2 cut(s) 779, 876
StyD4I CCNGG 7 cut(s) 286, 366, 511, 657, 1147, 1164, 1636
StyI CCWWGG 4 cut(s) 37, 645, 982, 1016
TaaI ACNGT 4 cut(s) 308, 555, 1049, 1095
TaiI ACGT 2 cut(s) 363, 536
TaqI TCGA 5 cut(s) 930, 1060, 1265, 1274, 1557
TasI AATT 7 cut(s) 749, 1007, 1109, 1437, 1452, 1513, 1656
TatI WGTACW 3 cut(s) 75, 1184, 1385
TauI GCSGC 1 cut(s) 244
TfiI GAWTC 4 cut(s) 461, 855, 1172, 1621
Tru1I TTAA 6 cut(s) 330, 518, 759, 771, 1455, 1487
Tru9I TTAA 6 cut(s) 330, 518, 759, 771, 1455, 1487
TscAI CASTG 3 cut(s) 730, 826, 1137
TseFI GTSAC 1 cut(s) 1530
TseI GCWGC 6 cut(s) 7, 467, 641, 1100, 1103, 1298
Tsp45I GTSAC 1 cut(s) 1530
TspDTI ATGAA 7 cut(s) 411, 423, 488, 610, 647, 1082, 1443
TspGWI ACGGA 1 cut(s) 12
TspRI CASTG 3 cut(s) 730, 826, 1137
Tth111I GACNNNGTC 1 cut(s) 535
VpaK11BI GGWCC 1 cut(s) 974
XagI CCTNNNNNAGG 1 cut(s) 120
XapI RAATTY 3 cut(s) 1109, 1513, 1656
XcmI CCANNNNNNNNNTGG 1 cut(s) 984
XhoI CTCGAG 1 cut(s) 1059
XmnI GAANNNNTTC 1 cut(s) 936
XspI CTAG 3 cut(s) 81, 1317, 1653
ZraI GACGTC 1 cut(s) 534
ZrmI AGTACT 1 cut(s) 1186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.