Rmu_sc0003357.1_g000005

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003357.1
OG0000302
Physical Location & Seq
Reverse (-)
15866 .. 17352
1487 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003357.1_g000005.1.cds

Sequence Viewer

Length: 1053 bp
atggcaagtggaacagcgtctggcatggcaccttatgcttacttggcaatgtaccaagtttgttatgatcacggttgttctgaaagtgacattttagctgttatggacactgctgttgccgatggagtggacgtgctctccctctcacttggtggtggctcagctgctttatattatgatggaatcacagttgccccatggattctaacggtcggagcaagcactattgacagaagcataagagcaatagcaaaacttggaaatgggaaagaatatgatggggaatccaatgattcaacagctctgtgcgattcaggaacccttaatggagttgaagggaaagtggtgctgtgtgaaagaagtggagaaattagaagaattgccagaggtgaagaagtcaaaagagcaggtggtgctgccatgatccttatgaacgaagaaacaaatggctatagcaccttagctgatggtcatgtgcttcccgcaacccatgtgagttatgctgcaggtgcaggtatgagcatcaaagcctacataaataaacagtacaaactcctcctcaacacctacaaccacaatcttgttcaaaggcactgtcatcggtacctcaatgtcatgccctcacctgagtggcattgcagctttgctcaaaagagctcacaccctgattggtcaccagctgccattaagtctgcaattttgacaactgctcaagtactaaacctcggcgtcaagcccattgtggatgaaacactcggtccagctgacatctttgccactggtgcaggccatgttaacccttcaagagcagatgaccgcggcctaatctttgacttagaaccagcggattacatcccctatctatgcgctaactcaacttacaggttagaggttggggtaccaagtaaaatcgcaataggcattgatactgaggagcttacatttacagaggttggacagaagattacataccaggtggcgtttaaccctctacaaggtgcagggaaggatgcactacttgttcagggatatctgagatgggtctctgattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

350

Amino Acids

37.4

Weight (kDa)

5.19

Isoelectric Point (pI)

35.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 398, 497
Acc36I ACCTGC 3 cut(s) 398, 497, 503
Acc65I GGTACC 2 cut(s) 603, 898
AccB1I GGYRCC 3 cut(s) 28, 603, 898
AccII CGCG 1 cut(s) 819
AciI CCGC 4 cut(s) 483, 817, 819, 845
AclWI GGATC 1 cut(s) 418
AcyI GRCGYC 1 cut(s) 729
AdeI CACNNNGTG 1 cut(s) 152
AfaI GTAC 5 cut(s) 53, 548, 605, 717, 900
AfiI CCNNNNNNNGG 1 cut(s) 995
AgsI TTSAA 4 cut(s) 297, 335, 587, 804
AjiI CACGTC 1 cut(s) 133
AjnI CCWGG 1 cut(s) 972
AleI CACNNNNGTG 1 cut(s) 628
AluBI AGCT 9 cut(s) 98, 164, 302, 464, 642, 657, 680, 764, 937
AluI AGCT 9 cut(s) 98, 164, 302, 464, 642, 657, 680, 764, 937
Alw21I GWGCWC 2 cut(s) 138, 659
Alw26I GTCTC 1 cut(s) 1048
AlwI GGATC 1 cut(s) 418
AlwNI CAGNNNCTG 1 cut(s) 20
AoxI GGCC 2 cut(s) 787, 820
ApeKI GCWGC 5 cut(s) 164, 416, 503, 639, 680
ArsI GACNNNNNNTTYG 2 cut(s) 580, 612
Asp718I GGTACC 2 cut(s) 603, 898
AspLEI GCGC 1 cut(s) 869
AspS9I GGNCC 1 cut(s) 758
AsuHPI GGTGA 3 cut(s) 401, 615, 666
AvaII GGWCC 1 cut(s) 758
BanI GGYRCC 3 cut(s) 28, 603, 898
BanII GRGCYC 1 cut(s) 659
BarI GAAGNNNNNNTAC 2 cut(s) 953, 985
Bbv12I GWGCWC 2 cut(s) 138, 659
BbvI GCAGC 5 cut(s) 151, 403, 490, 651, 667
BccI CCATC 5 cut(s) 116, 173, 272, 461, 1032
BcgI CGANNNNNNTGC 2 cut(s) 581, 615
BciT130I CCWGG 1 cut(s) 974
BclI TGATCA 1 cut(s) 67
BcoDI GTCTC 1 cut(s) 1048
BfmI CTRYAG 2 cut(s) 451, 504
BfuAI ACCTGC 3 cut(s) 398, 497, 503
BisI GCNGC 6 cut(s) 165, 417, 504, 640, 681, 820
BlpI GCTNAGC 1 cut(s) 160
BlsI GCNGC 6 cut(s) 166, 418, 505, 641, 682, 821
BmcAI AGTACT 1 cut(s) 717
Bme1390I CCNGG 1 cut(s) 974
Bme18I GGWCC 1 cut(s) 758
BmgBI CACGTC 1 cut(s) 133
BmgT120I GGNCC 1 cut(s) 758
BmiI GGNNCC 4 cut(s) 30, 319, 605, 900
BmrFI CCNGG 1 cut(s) 974
BmsI GCATC 2 cut(s) 531, 1000
Bpu10I CCTNAGC 1 cut(s) 460
Bpu1102I GCTNAGC 1 cut(s) 160
BpuEI CTTGAG 1 cut(s) 696
BsaHI GRCGYC 1 cut(s) 729
BsaI GGTCTC 1 cut(s) 1048
BsaJI CCNNGG 3 cut(s) 197, 724, 817
BsaXI ACNNNNNCTCC 4 cut(s) 117, 122, 147, 152
Bsc4I CCNNNNNNNGG 1 cut(s) 995
Bse1I ACTGG 1 cut(s) 784
Bse3DI GCAATG 2 cut(s) 54, 634
BseBI CCWGG 1 cut(s) 974
BseDI CCNNGG 3 cut(s) 197, 724, 817
BseGI GGATG 3 cut(s) 751, 852, 1015
BseLI CCNNNNNNNGG 1 cut(s) 995
BseMI GCAATG 2 cut(s) 54, 634
BseMII CTCAG 4 cut(s) 174, 618, 921, 1025
BseNI ACTGG 1 cut(s) 784
BseRI GAGGAG 3 cut(s) 545, 548, 947
BseXI GCAGC 5 cut(s) 151, 403, 490, 651, 667
BsgI GTGCAG 3 cut(s) 531, 804, 1020
Bsh1236I CGCG 1 cut(s) 819
Bsh1285I CGRYCG 1 cut(s) 213
BshFI GGCC 2 cut(s) 789, 822
BshNI GGYRCC 3 cut(s) 28, 603, 898
BsiEI CGRYCG 1 cut(s) 213
BsiHKAI GWGCWC 2 cut(s) 138, 659
BslI CCNNNNNNNGG 1 cut(s) 995
BsmAI GTCTC 1 cut(s) 1048
BsnI GGCC 2 cut(s) 789, 822
Bso31I GGTCTC 1 cut(s) 1048
Bsp1286I GDGCHC 2 cut(s) 138, 659
Bsp143I GATC 2 cut(s) 67, 423
Bsp1720I GCTNAGC 1 cut(s) 160
Bsp19I CCATGG 1 cut(s) 197
BspACI CCGC 4 cut(s) 483, 817, 819, 845
BspANI GGCC 2 cut(s) 789, 822
BspCNI CTCAG 4 cut(s) 173, 619, 922, 1026
BspFNI CGCG 1 cut(s) 819
BspLI GGNNCC 4 cut(s) 30, 319, 605, 900
BspMAI CTGCAG 1 cut(s) 508
BspMI ACCTGC 3 cut(s) 398, 497, 503
BspPI GGATC 1 cut(s) 418
BspT107I GGYRCC 3 cut(s) 28, 603, 898
BspTNI GGTCTC 1 cut(s) 1048
BsrDI GCAATG 2 cut(s) 54, 634
BsrI ACTGG 1 cut(s) 784
BssECI CCNNGG 3 cut(s) 197, 724, 817
BssMI GATC 2 cut(s) 67, 423
BssNI GRCGYC 1 cut(s) 729
BssT1I CCWWGG 1 cut(s) 197
Bst2UI CCWGG 1 cut(s) 974
Bst4CI ACNGT 5 cut(s) 74, 190, 211, 546, 596
BstACI GRCGYC 1 cut(s) 729
BstAPI GCANNNNNTGC 2 cut(s) 35, 413
BstC8I GCNNGC 2 cut(s) 220, 787
BstDEI CTNAG 6 cut(s) 160, 460, 627, 835, 930, 1034
BstDSI CCRYGG 2 cut(s) 197, 817
BstEII GGTNACC 1 cut(s) 672
BstENI CCTNNNNNAGG 1 cut(s) 993
BstF5I GGATG 3 cut(s) 751, 852, 1015
BstFNI CGCG 1 cut(s) 819
BstHHI GCGC 1 cut(s) 869
BstKTI GATC 2 cut(s) 70, 426
BstMAI GTCTC 1 cut(s) 1048
BstMBI GATC 2 cut(s) 67, 423
BstMCI CGRYCG 1 cut(s) 213
BstMWI GCNNNNNNNGC 5 cut(s) 35, 44, 413, 509, 782
BstNI CCWGG 1 cut(s) 974
BstPI GGTNACC 1 cut(s) 672
BstSCI CCNGG 1 cut(s) 972
BstSFI CTRYAG 2 cut(s) 451, 504
BstUI CGCG 1 cut(s) 819
BstV1I GCAGC 5 cut(s) 151, 403, 490, 651, 667
BsuRI GGCC 2 cut(s) 789, 822
BtgI CCRYGG 2 cut(s) 197, 817
BtrI CACGTC 1 cut(s) 133
BtsCI GGATG 3 cut(s) 751, 852, 1015
BtsI GCAGTG 1 cut(s) 108
BtsIMutI CAGTG 3 cut(s) 108, 592, 777
BveI ACCTGC 3 cut(s) 398, 497, 503
Cac8I GCNNGC 2 cut(s) 220, 787
CaiI CAGNNNCTG 1 cut(s) 20
CfoI GCGC 1 cut(s) 869
Cfr13I GGNCC 1 cut(s) 758
Cfr42I CCGCGG 1 cut(s) 820
CseI GACGC 2 cut(s) 6, 718
CsiI ACCWGGT 1 cut(s) 972
Csp6I GTAC 5 cut(s) 52, 547, 604, 716, 899
CviAII CATG 7 cut(s) 25, 198, 421, 473, 491, 616, 791
CviQI GTAC 5 cut(s) 52, 547, 604, 716, 899
DdeI CTNAG 6 cut(s) 160, 460, 627, 835, 930, 1034
DpnI GATC 2 cut(s) 69, 425
DpnII GATC 2 cut(s) 67, 423
DraIII CACNNNGTG 1 cut(s) 152
Ecl136II GAGCTC 1 cut(s) 657
Eco130I CCWWGG 1 cut(s) 197
Eco24I GRGCYC 1 cut(s) 659
Eco31I GGTCTC 1 cut(s) 1048
Eco32I GATATC 1 cut(s) 1031
Eco47I GGWCC 1 cut(s) 758
Eco53kI GAGCTC 1 cut(s) 657
Eco91I GGTNACC 1 cut(s) 672
EcoICRI GAGCTC 1 cut(s) 657
EcoNI CCTNNNNNAGG 1 cut(s) 993
EcoO65I GGTNACC 1 cut(s) 672
EcoRII CCWGG 1 cut(s) 972
EcoRV GATATC 1 cut(s) 1031
EcoT14I CCWWGG 1 cut(s) 197
EcoT38I GRGCYC 1 cut(s) 659
ErhI CCWWGG 1 cut(s) 197
FaeI CATG 7 cut(s) 28, 201, 424, 476, 494, 619, 794
FatI CATG 7 cut(s) 24, 197, 420, 472, 490, 615, 790
FauI CCCGC 1 cut(s) 490
FbaI TGATCA 1 cut(s) 67
Fnu4HI GCNGC 6 cut(s) 165, 417, 504, 640, 681, 820
FokI GGATG 3 cut(s) 758, 839, 1022
FriOI GRGCYC 1 cut(s) 659
Fsp4HI GCNGC 6 cut(s) 165, 417, 504, 640, 681, 820
GlaI GCGC 1 cut(s) 868
GluI GCNGC 6 cut(s) 165, 417, 504, 640, 681, 820
HaeIII GGCC 2 cut(s) 789, 822
HgaI GACGC 2 cut(s) 6, 718
HhaI GCGC 1 cut(s) 869
Hin1I GRCGYC 1 cut(s) 729
Hin1II CATG 7 cut(s) 28, 201, 424, 476, 494, 619, 794
Hin6I GCGC 1 cut(s) 867
HinP1I GCGC 1 cut(s) 867
HincII GTYRAC 1 cut(s) 796
HindII GTYRAC 1 cut(s) 796
HinfI GANTC 5 cut(s) 183, 202, 284, 293, 311
HpaI GTTAAC 1 cut(s) 796
HphI GGTGA 3 cut(s) 401, 615, 666
Hpy166II GTNNAC 2 cut(s) 130, 796
Hpy188I TCNGA 4 cut(s) 82, 215, 1035, 1048
Hpy188III TCNNGA 2 cut(s) 315, 804
Hpy8I GTNNAC 2 cut(s) 130, 796
HpyAV CCTTC 3 cut(s) 329, 810, 1000
HpyCH4III ACNGT 5 cut(s) 74, 190, 211, 546, 596
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 7 cut(s) 506, 512, 639, 695, 785, 1001, 1013
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 44, 413, 509, 782
HpyF3I CTNAG 6 cut(s) 160, 460, 627, 835, 930, 1034
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 729
Hsp92II CATG 7 cut(s) 28, 201, 424, 476, 494, 619, 794
HspAI GCGC 1 cut(s) 867
KpnI GGTACC 2 cut(s) 607, 902
Ksp22I TGATCA 1 cut(s) 67
KspAI GTTAAC 1 cut(s) 796
KspI CCGCGG 1 cut(s) 820
Kzo9I GATC 2 cut(s) 67, 423
LmnI GCTCC 2 cut(s) 215, 934
Lsp1109I GCAGC 5 cut(s) 151, 403, 490, 651, 667
LweI GCATC 2 cut(s) 531, 1000
MabI ACCWGGT 1 cut(s) 972
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 2 cut(s) 86, 672
MalI GATC 2 cut(s) 69, 425
MboI GATC 2 cut(s) 67, 423
MboII GAAGA 4 cut(s) 387, 404, 449, 973
MhlI GDGCHC 2 cut(s) 138, 659
MluCI AATT 3 cut(s) 369, 378, 696
MmeI TCCRAC 2 cut(s) 193, 934
MseI TTAA 4 cut(s) 324, 687, 795, 984
MslI CAYNNNNRTG 1 cut(s) 628
MspA1I CMGCKG 5 cut(s) 164, 680, 764, 819, 845
MspR9I CCNGG 1 cut(s) 974
MvaI CCWGG 1 cut(s) 974
MvnI CGCG 1 cut(s) 819
MwoI GCNNNNNNNGC 5 cut(s) 35, 44, 413, 509, 782
NcoI CCATGG 1 cut(s) 197
NdeII GATC 2 cut(s) 67, 423
NlaIII CATG 7 cut(s) 28, 201, 424, 476, 494, 619, 794
NlaIV GGNNCC 4 cut(s) 30, 319, 605, 900
NmeAIII GCCGAG 1 cut(s) 705
NmuCI GTSAC 2 cut(s) 86, 672
OliI CACNNNNGTG 1 cut(s) 628
PaqCI CACCTGC 2 cut(s) 398, 497
PfeI GAWTC 5 cut(s) 183, 202, 284, 293, 311
PkrI GCNGC 6 cut(s) 166, 418, 505, 641, 682, 821
Psp124BI GAGCTC 1 cut(s) 659
Psp6I CCWGG 1 cut(s) 972
PspEI GGTNACC 1 cut(s) 672
PspGI CCWGG 1 cut(s) 972
PspN4I GGNNCC 4 cut(s) 30, 319, 605, 900
PspPI GGNCC 1 cut(s) 758
PstI CTGCAG 1 cut(s) 508
PstNI CAGNNNCTG 1 cut(s) 20
PvuII CAGCTG 3 cut(s) 164, 680, 764
RsaI GTAC 5 cut(s) 53, 548, 605, 717, 900
RsaNI GTAC 5 cut(s) 52, 547, 604, 716, 899
RseI CAYNNNNRTG 1 cut(s) 628
SacI GAGCTC 1 cut(s) 659
SacII CCGCGG 1 cut(s) 820
SaqAI TTAA 4 cut(s) 324, 687, 795, 984
SatI GCNGC 6 cut(s) 165, 417, 504, 640, 681, 820
Sau3AI GATC 2 cut(s) 67, 423
Sau96I GGNCC 1 cut(s) 758
ScaI AGTACT 1 cut(s) 717
ScrFI CCNGG 1 cut(s) 974
SduI GDGCHC 2 cut(s) 138, 659
SexAI ACCWGGT 1 cut(s) 972
SfaNI GCATC 2 cut(s) 531, 1000
SfcI CTRYAG 2 cut(s) 451, 504
Sfr303I CCGCGG 1 cut(s) 820
SgrBI CCGCGG 1 cut(s) 820
SinI GGWCC 1 cut(s) 758
SmiMI CAYNNNNRTG 1 cut(s) 628
SmlI CTYRAG 1 cut(s) 711
SmoI CTYRAG 1 cut(s) 711
Sse9I AATT 3 cut(s) 369, 378, 696
SsiI CCGC 4 cut(s) 483, 817, 819, 845
SstI GAGCTC 1 cut(s) 659
StyD4I CCNGG 1 cut(s) 972
StyI CCWWGG 1 cut(s) 197
TaaI ACNGT 5 cut(s) 74, 190, 211, 546, 596
TaiI ACGT 1 cut(s) 135
TaqII GACCGA 1 cut(s) 746
TasI AATT 3 cut(s) 369, 378, 696
TatI WGTACW 2 cut(s) 546, 715
TauI GCSGC 1 cut(s) 822
TfiI GAWTC 5 cut(s) 183, 202, 284, 293, 311
Tru1I TTAA 4 cut(s) 324, 687, 795, 984
Tru9I TTAA 4 cut(s) 324, 687, 795, 984
TscAI CASTG 3 cut(s) 115, 599, 784
TseFI GTSAC 2 cut(s) 86, 672
TseI GCWGC 5 cut(s) 164, 416, 503, 639, 680
Tsp45I GTSAC 2 cut(s) 86, 672
TspDTI ATGAA 2 cut(s) 446, 762
TspRI CASTG 3 cut(s) 115, 599, 784
VpaK11BI GGWCC 1 cut(s) 758
XagI CCTNNNNNAGG 1 cut(s) 993
ZrmI AGTACT 1 cut(s) 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.