RLG00000003748

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000302
Physical Location & Seq
Forward (+)
52255372 .. 52258415
3044 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003748

Sequence Viewer

Length: 2301 bp
ATGCAAACTTACATTGTTTGGGTGAAAAAGCCTGTGAGGAGGCTATTTTCAGAATCTCATCATGAACATTTAGAGAGTTGGTACAAGTCATTTTTGCCCAAAAGTTTTGCAAACTCAAAGGAGGTGATGGACCAACGAATGGTTTACGCATACCACCATGTGGCTACTGGCTTTGCAGCAAAGCTTACAGCGGAGGAAGTAAGAGAAATGGAGACCAAGGATGGTTTTATATCAGCTCATCAAGATAGGATTCTCCCTCTGCACACTACCCACAGTCCGAATTTCTTGGGGTTGTACCAAGATTTGGGACTTTGGAAAGGGTCTAATTATGGTGAAGGAATCATTATTGGAGTTTTGGATTCTGGGATAACACCAGGTCATCCCTCATTCAGCGATGAAGGAGTGGGACCTCCTCCGGCTAAATGGAAAGGCAAGTGTGATTTCAATGGCACATTCTGCAACAACAAGCTTATTGGTGCCAGAAGTTTCCAGGGTGGTAAAACTACTGGAGCTCCACCAGTTGACCTTGATGGCCATGGAACCCACACGTCGAGCACAGCTGGAGGAAATTTTGTGAAAGGCGCCAACGTGTTTGGAATGGCAAAGGGAATAGCCTCTGGCATGGCACCTTATGCTCACTTGGCAATGTACCAAATTTGTTCTGAACAGGGTTGTTCTGAAAGTGACATTGTAGCTGCTATGGACACTGCTGTTGCCGATGGAGTGGACGTGCTCTCCCTCTCACTTGGCGGTGGCTCAGCTCCTTTCTACGCAGACGGAATCGCAGTCGGTGCATTTGGAGCAATTCAGAAGGGAATTTTTGTTAGCTGCTCAGCTGGAAATGATGGGCCTGTCTATGGTTCTTTATCCAATGAAGCGCCATGGATTCTAACCGTCGGAGCAAGCACCATTGACAGAAGCATAAAGGCAACAGCAAAACTTGGAAATGGGCAGGAATATGATGGGGAATCGTTATTCCAGGATAAAAATTTTAGTTCAAATTTGTTACCTCTTGTTTATGCCGGTGCATATAGCAATGATTCAACAGCTCTGTGCGATGTAGGAACCCTTAATGGAGTTGAAGGGAAAGTGGTGCTGTGTGAAAGAGGTGGAGGAATTGGAAGAATTGCCAAAGGAGAAGAAGTCAAAAGAGCAGGTGGTGCTGCCATGATCCTCATGAACGAAGACACTGATGGCTATAGCACCTTAGCTGATGCTCATGTGCTTCCTGCAACACATGTGAGTTATGCTGCAGGTTTGAGCATCAAAGCCTACATAAACTCCACCTCAACACCTATGGCCACAATCTTGTTCAAAGGCACTGTCATCGGTGATCAAAATGCTCCCGCAGTCACTTCCTTTTCATCAAGAGGACCCAGCCTGGCGAGCACAGGAATTTTGAAACCTGACATCATTGGTCCCGGTGTTAGCATCTTAGCTGCATGGCCTGTTTCGGTGGACAATGGCACAGAATCTACGGCAACATTTAACGTGGTTTCAGGTACTTCAATGTCATGCCCTCACCTGAGTGGCATTGCAGCTTTGCTCAAGAGCTCACACCCCGACTGGTCACCAGCTGCCATCAAGTCTGCAATTGTGACAACCGCTGACATAGTAAACCTCGGAGGCAAACCTATTATTGATGAAACTCTTGCTCCAGCAGACATCTTTGCCACTGGTGCAGGACATGTTAATCCTCCAAAAGCAGATGACCCCGGGCTAATCTACGACACAAAACCAACAGATTACATTCCCTACCTGTGCGGTTTGAACTACACAGACGATCAGATACAGATAATCACCCAACAAACAGTGAAGTGCTCTGAAGTAGGAGTCATACCTGAAGCGCAGCTAAATTATCCTTCATTTTCTATTATATTCCAGGACTTGTCTCAGTCTCAAGATTACACAAGGACGGTGACAAATGTTGGACCAGCTAATTCAACTTACAACTTGGATCTTAAGTTACCAGGTAAAATCGGCATGAGTGTGAGTCCTTCGCAGCTTATGTTTACAGAAGTTGGCCAGACAATTACATACCGAGCAGAGTTTATCCCGGAAGATGGGGCAGGGAAGGATGCACCATTTTCTCAGGGATATTTAAGATGGTTCTCTGATCAGCATTCTGTCACTAGCCCCATATCTGTGATCTTCGACACCCAGCAAGCACAGTTCCAGGCCAGTTTCCTCGAACAGTTGGTGAGTGCCAGCGGTGCTGCTGAAATAATACTTACCGCTTGGCTAAACATTGAATTCACCAAATGCCACACTTCCCTTGAGACTAATGTGTATATCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

767

Amino Acids

81.1

Weight (kDa)

5.19

Isoelectric Point (pI)

29.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 3 - 88 1.1e-09 Peptidase inhibitor I9
Peptidase_S8 PF00082 111 - 536 1.5e-45 Subtilase family
PA PF02225 337 - 422 1.5e-13 PA domain
fn3_6 PF17766 617 - 716 3.5e-24 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1145
Acc36I ACCTGC 2 cut(s) 1145, 1244
AccB1I GGYRCC 3 cut(s) 476, 581, 625
AccB7I CCANNNNNTGG 3 cut(s) 139, 160, 304
AciI CCGC 7 cut(s) 191, 750, 1347, 1605, 1764, 2211, 2235
AclWI GGATC 2 cut(s) 1165, 1965
AcoI YGGCCR 3 cut(s) 532, 1299, 2023
AcsI RAATTY 8 cut(s) 280, 568, 654, 816, 988, 1000, 1395, 2252
AcuI CTGAAG 2 cut(s) 1845, 1863
AcyI GRCGYC 1 cut(s) 582
AdeI CACNNNGTG 1 cut(s) 160
AfaI GTAC 4 cut(s) 83, 296, 650, 1504
AfiI CCNNNNNNNGG 5 cut(s) 139, 160, 304, 857, 2063
AflII CTTAAG 1 cut(s) 1961
AflIII ACRYGT 4 cut(s) 546, 588, 1237, 1687
AjiI CACGTC 2 cut(s) 549, 730
AjnI CCWGG 7 cut(s) 373, 489, 978, 1380, 1881, 1969, 2175
AleI CACNNNNGTG 1 cut(s) 1527
Alw21I GWGCWC 6 cut(s) 514, 557, 735, 1391, 1556, 1823
Alw26I GTCTC 4 cut(s) 206, 1896, 1902, 2273
AlwI GGATC 2 cut(s) 1165, 1965
Ama87I CYCGRG 1 cut(s) 1715
AoxI GGCC 6 cut(s) 532, 848, 1299, 1445, 2023, 2178
ApoI RAATTY 8 cut(s) 280, 568, 654, 816, 988, 1000, 1395, 2252
ArsI GACNNNNNNTTYG 2 cut(s) 1308, 1340
AspLEI GCGC 3 cut(s) 584, 880, 1849
AspS9I GGNCC 6 cut(s) 130, 407, 848, 1373, 1418, 1931
AsuC2I CCSGG 4 cut(s) 1422, 1716, 1717, 2057
AvaI CYCGRG 1 cut(s) 1715
AvaII GGWCC 5 cut(s) 130, 407, 1373, 1418, 1931
BalI TGGCCA 3 cut(s) 534, 1301, 2025
BanI GGYRCC 3 cut(s) 476, 581, 625
BanII GRGCYC 2 cut(s) 514, 1556
BbsI GAAGAC 1 cut(s) 1191
Bbv12I GWGCWC 6 cut(s) 514, 557, 735, 1391, 1556, 1823
BceAI ACGGC 1 cut(s) 1494
BcgI CGANNNNNNTGC 2 cut(s) 1309, 1343
BciT130I CCWGG 7 cut(s) 375, 491, 980, 1382, 1883, 1971, 2177
BclI TGATCA 2 cut(s) 1333, 2116
BcnI CCSGG 4 cut(s) 1422, 1716, 1717, 2057
BcoDI GTCTC 4 cut(s) 206, 1896, 1902, 2273
BfaI CTAG 1 cut(s) 2133
BfmI CTRYAG 2 cut(s) 1198, 1251
BfoI RGCGCY 2 cut(s) 585, 881
BfrI CTTAAG 1 cut(s) 1961
BfuAI ACCTGC 2 cut(s) 1145, 1244
BlpI GCTNAGC 2 cut(s) 757, 832
Bme18I GGWCC 5 cut(s) 130, 407, 1373, 1418, 1931
BmeT110I CYCGRG 1 cut(s) 1715
BmgBI CACGTC 2 cut(s) 549, 730
BmgT120I GGNCC 6 cut(s) 130, 407, 848, 1373, 1418, 1931
BmiI GGNNCC 8 cut(s) 408, 478, 541, 583, 627, 1066, 1375, 1420
BmsI GCATC 4 cut(s) 1204, 1272, 1440, 2068
BpiI GAAGAC 1 cut(s) 1191
BpmI CTGGAG 3 cut(s) 528, 582, 1641
Bpu10I CCTNAGC 1 cut(s) 1207
Bpu1102I GCTNAGC 2 cut(s) 757, 832
BpuEI CTTGAG 3 cut(s) 1532, 1884, 2297
BpuMI CCSGG 4 cut(s) 1422, 1716, 1717, 2057
BsaHI GRCGYC 1 cut(s) 582
BsaI GGTCTC 1 cut(s) 206
BsaJI CCNNGG 7 cut(s) 216, 490, 535, 881, 1621, 1714, 1715
Bsc4I CCNNNNNNNGG 5 cut(s) 139, 160, 304, 857, 2063
Bse118I RCCGGY 1 cut(s) 1022
Bse1I ACTGG 6 cut(s) 172, 511, 518, 1571, 1681, 2181
Bse3DI GCAATG 3 cut(s) 651, 1042, 1533
BseBI CCWGG 7 cut(s) 375, 491, 980, 1382, 1883, 1971, 2177
BseDI CCNNGG 7 cut(s) 216, 490, 535, 881, 1621, 1714, 1715
BseGI GGATG 3 cut(s) 226, 379, 2083
BseLI CCNNNNNNNGG 5 cut(s) 139, 160, 304, 857, 2063
BseMI GCAATG 3 cut(s) 651, 1042, 1533
BseMII CTCAG 5 cut(s) 771, 846, 1517, 1907, 2105
BseNI ACTGG 6 cut(s) 172, 511, 518, 1571, 1681, 2181
BseRI GAGGAG 2 cut(s) 52, 402
BseYI CCCAGC 2 cut(s) 1376, 2160
BsgI GTGCAG 2 cut(s) 245, 1701
BshFI GGCC 6 cut(s) 534, 850, 1301, 1447, 2025, 2180
BshNI GGYRCC 3 cut(s) 476, 581, 625
BsiHKAI GWGCWC 6 cut(s) 514, 557, 735, 1391, 1556, 1823
BsiHKCI CYCGRG 1 cut(s) 1715
BsiSI CCGG 5 cut(s) 416, 1023, 1422, 1716, 2057
BslFI GGGAC 3 cut(s) 321, 420, 1404
BslI CCNNNNNNNGG 5 cut(s) 139, 160, 304, 857, 2063
BsmAI GTCTC 4 cut(s) 206, 1896, 1902, 2273
BsmFI GGGAC 3 cut(s) 321, 420, 1404
BsmI GAATGC 1 cut(s) 2122
BsnI GGCC 6 cut(s) 534, 850, 1301, 1447, 2025, 2180
Bso31I GGTCTC 1 cut(s) 206
BsoBI CYCGRG 1 cut(s) 1715
Bsp1286I GDGCHC 6 cut(s) 514, 557, 735, 1391, 1556, 1823
Bsp143I GATC 6 cut(s) 1170, 1333, 1783, 1957, 2116, 2148
Bsp1720I GCTNAGC 2 cut(s) 757, 832
Bsp19I CCATGG 2 cut(s) 535, 881
BspACI CCGC 7 cut(s) 191, 750, 1347, 1605, 1764, 2211, 2235
BspANI GGCC 6 cut(s) 534, 850, 1301, 1447, 2025, 2180
BspCNI CTCAG 5 cut(s) 770, 845, 1518, 1906, 2104
BspHI TCATGA 2 cut(s) 61, 1176
BspLI GGNNCC 8 cut(s) 408, 478, 541, 583, 627, 1066, 1375, 1420
BspMAI CTGCAG 1 cut(s) 1255
BspMI ACCTGC 2 cut(s) 1145, 1244
BspPI GGATC 2 cut(s) 1165, 1965
BspT107I GGYRCC 3 cut(s) 476, 581, 625
BspTI CTTAAG 1 cut(s) 1961
BspTNI GGTCTC 1 cut(s) 206
BsrDI GCAATG 3 cut(s) 651, 1042, 1533
BsrFI RCCGGY 1 cut(s) 1022
BsrI ACTGG 6 cut(s) 172, 511, 518, 1571, 1681, 2181
BssAI RCCGGY 1 cut(s) 1022
BssECI CCNNGG 7 cut(s) 216, 490, 535, 881, 1621, 1714, 1715
BssMI GATC 6 cut(s) 1170, 1333, 1783, 1957, 2116, 2148
BssNI GRCGYC 1 cut(s) 582
BssT1I CCWWGG 3 cut(s) 216, 535, 881
Bst2UI CCWGG 7 cut(s) 375, 491, 980, 1382, 1883, 1971, 2177
Bst4CI ACNGT 7 cut(s) 275, 895, 1324, 1813, 1918, 2172, 2196
BstACI GRCGYC 1 cut(s) 582
BstAFI CTTAAG 1 cut(s) 1961
BstAPI GCANNNNNTGC 4 cut(s) 456, 632, 791, 1160
BstC8I GCNNGC 4 cut(s) 904, 1387, 2166, 2209
BstDEI CTNAG 7 cut(s) 757, 832, 1207, 1435, 1526, 1893, 2091
BstDSI CCRYGG 2 cut(s) 535, 881
BstEII GGTNACC 1 cut(s) 1569
BstF5I GGATG 3 cut(s) 226, 379, 2083
BstH2I RGCGCY 2 cut(s) 585, 881
BstHHI GCGC 3 cut(s) 584, 880, 1849
BstKTI GATC 6 cut(s) 1173, 1336, 1786, 1960, 2119, 2151
BstMAI GTCTC 4 cut(s) 206, 1896, 1902, 2273
BstMBI GATC 6 cut(s) 1170, 1333, 1783, 1957, 2116, 2148
BstMWI GCNNNNNNNGC 9 cut(s) 456, 632, 641, 791, 800, 1160, 1386, 1679, 2213
BstNI CCWGG 7 cut(s) 375, 491, 980, 1382, 1883, 1971, 2177
BstNSI RCATGY 2 cut(s) 1241, 1691
BstPI GGTNACC 1 cut(s) 1569
BstSFI CTRYAG 2 cut(s) 1198, 1251
BstV2I GAAGAC 1 cut(s) 1191
BstX2I RGATCY 1 cut(s) 1957
BstYI RGATCY 1 cut(s) 1957
BsuRI GGCC 6 cut(s) 534, 850, 1301, 1447, 2025, 2180
BtgI CCRYGG 2 cut(s) 535, 881
BtgZI GCGATG 2 cut(s) 408, 1071
BtrI CACGTC 2 cut(s) 549, 730
BtsCI GGATG 3 cut(s) 226, 379, 2083
BtsI GCAGTG 1 cut(s) 705
BtsIMutI CAGTG 5 cut(s) 705, 1188, 1320, 1674, 1818
BveI ACCTGC 2 cut(s) 1145, 1244
Cac8I GCNNGC 4 cut(s) 904, 1387, 2166, 2209
CciI TCATGA 2 cut(s) 61, 1176
CfoI GCGC 3 cut(s) 584, 880, 1849
Cfr10I RCCGGY 1 cut(s) 1022
Cfr13I GGNCC 6 cut(s) 130, 407, 848, 1373, 1418, 1931
Cfr9I CCCGGG 1 cut(s) 1715
CsiI ACCWGGT 2 cut(s) 373, 1969
Csp6I GTAC 4 cut(s) 82, 295, 649, 1503
CviQI GTAC 4 cut(s) 82, 295, 649, 1503
DdeI CTNAG 7 cut(s) 757, 832, 1207, 1435, 1526, 1893, 2091
DinI GGCGCC 1 cut(s) 583
DpnI GATC 6 cut(s) 1172, 1335, 1785, 1959, 2118, 2150
DpnII GATC 6 cut(s) 1170, 1333, 1783, 1957, 2116, 2148
DraIII CACNNNGTG 1 cut(s) 160
EaeI YGGCCR 3 cut(s) 532, 1299, 2023
Ecl136II GAGCTC 2 cut(s) 512, 1554
Eco130I CCWWGG 3 cut(s) 216, 535, 881
Eco24I GRGCYC 2 cut(s) 514, 1556
Eco31I GGTCTC 1 cut(s) 206
Eco47I GGWCC 5 cut(s) 130, 407, 1373, 1418, 1931
Eco53kI GAGCTC 2 cut(s) 512, 1554
Eco57I CTGAAG 2 cut(s) 1845, 1863
Eco88I CYCGRG 1 cut(s) 1715
Eco91I GGTNACC 1 cut(s) 1569
EcoICRI GAGCTC 2 cut(s) 512, 1554
EcoO109I RGGNCCY 2 cut(s) 407, 1373
EcoO65I GGTNACC 1 cut(s) 1569
EcoRI GAATTC 1 cut(s) 2252
EcoRII CCWGG 7 cut(s) 373, 489, 978, 1380, 1881, 1969, 2175
EcoT14I CCWWGG 3 cut(s) 216, 535, 881
EcoT38I GRGCYC 2 cut(s) 514, 1556
EgeI GGCGCC 1 cut(s) 583
EheI GGCGCC 1 cut(s) 583
ErhI CCWWGG 3 cut(s) 216, 535, 881
FaqI GGGAC 3 cut(s) 321, 420, 1404
FauI CCCGC 1 cut(s) 1354
FbaI TGATCA 2 cut(s) 1333, 2116
FokI GGATG 3 cut(s) 233, 366, 2090
FriOI GRGCYC 2 cut(s) 514, 1556
FspBI CTAG 1 cut(s) 2133
GlaI GCGC 3 cut(s) 583, 879, 1848
GsaI CCCAGC 2 cut(s) 1380, 2164
GsuI CTGGAG 3 cut(s) 528, 582, 1641
HaeII RGCGCY 2 cut(s) 585, 881
HaeIII GGCC 6 cut(s) 534, 850, 1301, 1447, 2025, 2180
HapII CCGG 5 cut(s) 416, 1023, 1422, 1716, 2057
HhaI GCGC 3 cut(s) 584, 880, 1849
Hin1I GRCGYC 1 cut(s) 582
Hin6I GCGC 3 cut(s) 582, 878, 1847
HinP1I GCGC 3 cut(s) 582, 878, 1847
HincII GTYRAC 1 cut(s) 523
HindII GTYRAC 1 cut(s) 523
HindIII AAGCTT 2 cut(s) 182, 467
HpaII CCGG 5 cut(s) 416, 1023, 1422, 1716, 2057
Hpy166II GTNNAC 6 cut(s) 145, 523, 727, 1459, 1618, 2013
Hpy188III TCNNGA 6 cut(s) 62, 242, 1177, 1368, 1549, 1901
Hpy8I GTNNAC 6 cut(s) 145, 523, 727, 1459, 1618, 2013
Hpy99I CGWCG 2 cut(s) 553, 899
HpyAV CCTTC 7 cut(s) 329, 392, 805, 1076, 1872, 2007, 2068
HpyCH4III ACNGT 7 cut(s) 275, 895, 1324, 1813, 1918, 2172, 2196
HpyCH4IV ACGT 4 cut(s) 548, 588, 729, 1491
HpyF10VI GCNNNNNNNGC 9 cut(s) 456, 632, 641, 791, 800, 1160, 1386, 1679, 2213
HpyF3I CTNAG 7 cut(s) 757, 832, 1207, 1435, 1526, 1893, 2091
HpySE526I ACGT 4 cut(s) 548, 588, 729, 1491
Hsp92I GRCGYC 1 cut(s) 582
HspAI GCGC 3 cut(s) 582, 878, 1847
KasI GGCGCC 1 cut(s) 581
Ksp22I TGATCA 2 cut(s) 1333, 2116
Kzo9I GATC 6 cut(s) 1170, 1333, 1783, 1957, 2116, 2148
LmnI GCTCC 7 cut(s) 509, 517, 766, 800, 899, 1348, 1660
LweI GCATC 4 cut(s) 1204, 1272, 1440, 2068
MabI ACCWGGT 2 cut(s) 373, 1969
MaeI CTAG 1 cut(s) 2133
MaeII ACGT 4 cut(s) 548, 588, 729, 1491
MaeIII GTNAC 8 cut(s) 683, 1005, 1351, 1569, 1597, 1918, 1965, 2128
MalI GATC 6 cut(s) 1172, 1335, 1785, 1959, 2118, 2150
MboI GATC 6 cut(s) 1170, 1333, 1783, 1957, 2116, 2148
MboII GAAGA 5 cut(s) 1134, 1151, 1196, 2072, 2143
MfeI CAATTG 1 cut(s) 1593
MflI RGATCY 1 cut(s) 1957
MhlI GDGCHC 6 cut(s) 514, 557, 735, 1391, 1556, 1823
MlsI TGGCCA 3 cut(s) 534, 1301, 2025
MluNI TGGCCA 3 cut(s) 534, 1301, 2025
Mly113I GGCGCC 1 cut(s) 582
MlyI GAGTC 2 cut(s) 1842, 2002
MmeI TCCRAC 2 cut(s) 877, 1909
Mox20I TGGCCA 3 cut(s) 534, 1301, 2025
MscI TGGCCA 3 cut(s) 534, 1301, 2025
MseI TTAA 5 cut(s) 1071, 1488, 1692, 1962, 2102
MslI CAYNNNNRTG 3 cut(s) 1527, 1988, 2144
Msp20I TGGCCA 3 cut(s) 534, 1301, 2025
MspA1I CMGCKG 6 cut(s) 191, 560, 836, 1577, 1607, 2211
MspCI CTTAAG 1 cut(s) 1961
MspI CCGG 5 cut(s) 416, 1023, 1422, 1716, 2057
MunI CAATTG 1 cut(s) 1593
Mva1269I GAATGC 1 cut(s) 2122
MvaI CCWGG 7 cut(s) 375, 491, 980, 1382, 1883, 1971, 2177
MwoI GCNNNNNNNGC 9 cut(s) 456, 632, 641, 791, 800, 1160, 1386, 1679, 2213
NarI GGCGCC 1 cut(s) 582
NciI CCSGG 4 cut(s) 1422, 1716, 1717, 2057
NcoI CCATGG 2 cut(s) 535, 881
NdeII GATC 6 cut(s) 1170, 1333, 1783, 1957, 2116, 2148
NlaIV GGNNCC 8 cut(s) 408, 478, 541, 583, 627, 1066, 1375, 1420
NmuCI GTSAC 6 cut(s) 683, 1351, 1569, 1597, 1918, 2128
NspI RCATGY 2 cut(s) 1241, 1691
OliI CACNNNNGTG 1 cut(s) 1527
PagI TCATGA 2 cut(s) 61, 1176
PaqCI CACCTGC 1 cut(s) 1145
PciI ACATGT 2 cut(s) 1237, 1687
PctI GAATGC 1 cut(s) 2122
PfeI GAWTC 9 cut(s) 53, 250, 339, 359, 780, 886, 968, 1040, 1472
PflMI CCANNNNNTGG 3 cut(s) 139, 160, 304
PfoI TCCNGGA 3 cut(s) 978, 1881, 2055
PleI GAGTC 2 cut(s) 1841, 2001
PluTI GGCGCC 1 cut(s) 585
PpsI GAGTC 2 cut(s) 1841, 2001
PpuMI RGGWCCY 2 cut(s) 407, 1373
PscI ACATGT 2 cut(s) 1237, 1687
Psp124BI GAGCTC 2 cut(s) 514, 1556
Psp5II RGGWCCY 2 cut(s) 407, 1373
Psp6I CCWGG 7 cut(s) 373, 489, 978, 1380, 1881, 1969, 2175
PspEI GGTNACC 1 cut(s) 1569
PspFI CCCAGC 2 cut(s) 1376, 2160
PspGI CCWGG 7 cut(s) 373, 489, 978, 1380, 1881, 1969, 2175
PspN4I GGNNCC 8 cut(s) 408, 478, 541, 583, 627, 1066, 1375, 1420
PspPI GGNCC 6 cut(s) 130, 407, 848, 1373, 1418, 1931
PspPPI RGGWCCY 2 cut(s) 407, 1373
PstI CTGCAG 1 cut(s) 1255
PsuI RGATCY 1 cut(s) 1957
PvuII CAGCTG 3 cut(s) 560, 836, 1577
RsaI GTAC 4 cut(s) 83, 296, 650, 1504
RsaNI GTAC 4 cut(s) 82, 295, 649, 1503
RseI CAYNNNNRTG 3 cut(s) 1527, 1988, 2144
SacI GAGCTC 2 cut(s) 514, 1556
SaqAI TTAA 5 cut(s) 1071, 1488, 1692, 1962, 2102
Sau3AI GATC 6 cut(s) 1170, 1333, 1783, 1957, 2116, 2148
Sau96I GGNCC 6 cut(s) 130, 407, 848, 1373, 1418, 1931
SchI GAGTC 2 cut(s) 1842, 2002
SduI GDGCHC 6 cut(s) 514, 557, 735, 1391, 1556, 1823
SexAI ACCWGGT 2 cut(s) 373, 1969
SfaNI GCATC 4 cut(s) 1204, 1272, 1440, 2068
SfcI CTRYAG 2 cut(s) 1198, 1251
SfoI GGCGCC 1 cut(s) 583
SinI GGWCC 5 cut(s) 130, 407, 1373, 1418, 1931
SmaI CCCGGG 1 cut(s) 1717
SmiMI CAYNNNNRTG 3 cut(s) 1527, 1988, 2144
SmlI CTYRAG 4 cut(s) 1547, 1899, 1961, 2276
SmoI CTYRAG 4 cut(s) 1547, 1899, 1961, 2276
SsiI CCGC 7 cut(s) 191, 750, 1347, 1605, 1764, 2211, 2235
SspDI GGCGCC 1 cut(s) 581
SspMI CTAG 1 cut(s) 2133
SstI GAGCTC 2 cut(s) 514, 1556
StyI CCWWGG 3 cut(s) 216, 535, 881
TaaI ACNGT 7 cut(s) 275, 895, 1324, 1813, 1918, 2172, 2196
TaiI ACGT 4 cut(s) 551, 591, 732, 1494
TaqI TCGA 3 cut(s) 551, 2154, 2190
TfiI GAWTC 9 cut(s) 53, 250, 339, 359, 780, 886, 968, 1040, 1472
Tru1I TTAA 5 cut(s) 1071, 1488, 1692, 1962, 2102
Tru9I TTAA 5 cut(s) 1071, 1488, 1692, 1962, 2102
TscAI CASTG 5 cut(s) 712, 1195, 1327, 1681, 1818
TseFI GTSAC 6 cut(s) 683, 1351, 1569, 1597, 1918, 2128
Tsp45I GTSAC 6 cut(s) 683, 1351, 1569, 1597, 1918, 2128
TspDTI ATGAA 7 cut(s) 78, 411, 888, 1193, 1353, 1659, 1854
TspGWI ACGGA 1 cut(s) 792
TspMI CCCGGG 1 cut(s) 1715
TspRI CASTG 5 cut(s) 712, 1195, 1327, 1681, 1818
Van91I CCANNNNNTGG 3 cut(s) 139, 160, 304
Vha464I CTTAAG 1 cut(s) 1961
VpaK11BI GGWCC 5 cut(s) 130, 407, 1373, 1418, 1931
XapI RAATTY 8 cut(s) 280, 568, 654, 816, 988, 1000, 1395, 2252
XceI RCATGY 2 cut(s) 1241, 1691
XcmI CCANNNNNNNNNTGG 1 cut(s) 164
XmaI CCCGGG 1 cut(s) 1715
XspI CTAG 1 cut(s) 2133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.