Rh7AG183300

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000302
Physical Location & Seq
Reverse (-)
16302809 .. 16305064
2256 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG183300.1

Sequence Viewer

Length: 864 bp
ATGGCCGTGATTCCATTTCTTTTTCTTGTTTTCATGCTTAGCTTTTCTCATGGGATTGCTCAGGAAACCGAGCTTTCTCCTGTCCCTACGAAAACGAGCAATTTACAGACATACATTGTCCATGTAATGCAACCAGAGGGTAAAGTTTTTGCCCAAAGAGAAGACCTGGTGAGCTGGCACAAATCTTTTCTGCCATCTATGACAGCAAGCTCTGATGAGCAAACACGGTTGCTTTACTCCTACAAAACTGTGATCAGTGGTTTTTCAGCAAGGCTAACTCAAGAGGAAGTGAAAGCGATGGAACAGATGAATGGTTTTGTGGCGGCACATCCTGAACGAGTGTTTCGTCGGAAAACCACACACACTCCCAACTTCTTGGGGCTGAACCAACAAGCTGGAATTTGGAAAGACTCAAACTTTGGGAAGGGAGTGATCATTGGAGTACTGGATGGCGGAGTATTCCCCAGCCACCCTTCATTCAGTGGTGCAGGAATTCCACCTCCACCTGCTAAATGGAAAGGGAGACCAATAAAGTGCTCAAAGGTATCAAGCATCCCTGAAGGAGAGCTGAACTACCCTTCATTTTCTGTCAAGCTGGGACCATCTCAGACATTCACAAGAACTGTGACAAATGTTGGTGCCTCATATTCCACTTATTCGGTCAAGGTATATGCACCACTAGGAACCTATGTGACTGTCAAACCCAGCACGCTTTACTTTACAGAGGTGAACCAGAAGGCGTCATATTCAGTGACATTCAGTCGTACTTCAGGAGGTAAAGCTGGTGCATTCACACAGGGATTTATAACATGGGCTTCTACTAATCACGTTGTCCGGAGTCCAGTTTCTGTTTTATTTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

287

Amino Acids

31.5

Weight (kDa)

9.71

Isoelectric Point (pI)

32.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 37 - 115 5.1e-14 Peptidase inhibitor I9
fn3_6 PF17766 189 - 284 6.8e-29 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 806
AarI CACCTGC 1 cut(s) 514
Acc36I ACCTGC 1 cut(s) 514
AccB1I GGYRCC 1 cut(s) 638
AccIII TCCGGA 1 cut(s) 834
AciI CCGC 2 cut(s) 323, 453
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 399, 492
AcuI CTGAAG 2 cut(s) 579, 753
AcyI GRCGYC 1 cut(s) 740
AfaI GTAC 2 cut(s) 444, 766
AhdI GACNNNNNGTC 1 cut(s) 759
AjnI CCWGG 1 cut(s) 165
AluBI AGCT 8 cut(s) 42, 73, 174, 210, 395, 568, 595, 782
AluI AGCT 8 cut(s) 42, 73, 174, 210, 395, 568, 595, 782
Alw21I GWGCWC 1 cut(s) 539
Alw26I GTCTC 1 cut(s) 517
AlwNI CAGNNNCTG 1 cut(s) 848
Aor13HI TCCGGA 1 cut(s) 834
AoxI GGCC 1 cut(s) 3
ApoI RAATTY 2 cut(s) 399, 492
ArsI GACNNNNNNTTYG 2 cut(s) 401, 433
AspS9I GGNCC 1 cut(s) 599
AsuHPI GGTGA 2 cut(s) 181, 739
AvaII GGWCC 1 cut(s) 599
BanI GGYRCC 1 cut(s) 638
BbsI GAAGAC 1 cut(s) 168
Bbv12I GWGCWC 1 cut(s) 539
BccI CCATC 4 cut(s) 202, 292, 443, 610
BciT130I CCWGG 1 cut(s) 167
BclI TGATCA 2 cut(s) 252, 432
BcoDI GTCTC 1 cut(s) 517
BfaI CTAG 1 cut(s) 680
BfuAI ACCTGC 1 cut(s) 514
BisI GCNGC 1 cut(s) 324
BlpI GCTNAGC 1 cut(s) 38
BlsI GCNGC 1 cut(s) 325
BmcAI AGTACT 1 cut(s) 444
Bme1390I CCNGG 1 cut(s) 167
Bme18I GGWCC 1 cut(s) 599
BmeRI GACNNNNNGTC 1 cut(s) 759
BmgT120I GGNCC 1 cut(s) 599
BmiI GGNNCC 3 cut(s) 600, 640, 685
BmrFI CCNGG 1 cut(s) 167
BmsI GCATC 1 cut(s) 561
BpiI GAAGAC 1 cut(s) 168
Bpu10I CCTNAGC 1 cut(s) 60
Bpu1102I GCTNAGC 1 cut(s) 38
BpuEI CTTGAG 1 cut(s) 264
BsaHI GRCGYC 1 cut(s) 740
BsaI GGTCTC 1 cut(s) 517
BsaWI WCCGGW 1 cut(s) 834
BsaXI ACNNNNNCTCC 2 cut(s) 349, 379
Bse1I ACTGG 2 cut(s) 450, 842
BseAI TCCGGA 1 cut(s) 834
BseBI CCWGG 1 cut(s) 167
BseGI GGATG 3 cut(s) 328, 454, 552
BseMII CTCAG 2 cut(s) 74, 620
BseNI ACTGG 2 cut(s) 450, 842
BseYI CCCAGC 3 cut(s) 464, 595, 704
BsgI GTGCAG 1 cut(s) 507
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 638
BsiHKAI GWGCWC 1 cut(s) 539
BsiSI CCGG 1 cut(s) 835
BslFI GGGAC 2 cut(s) 68, 612
BsmAI GTCTC 1 cut(s) 517
BsmFI GGGAC 2 cut(s) 68, 612
BsmI GAATGC 1 cut(s) 788
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 517
Bsp1286I GDGCHC 1 cut(s) 539
Bsp13I TCCGGA 1 cut(s) 834
Bsp143I GATC 2 cut(s) 252, 432
Bsp1720I GCTNAGC 1 cut(s) 38
BspACI CCGC 2 cut(s) 323, 453
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 73, 619
BspEI TCCGGA 1 cut(s) 834
BspLI GGNNCC 3 cut(s) 600, 640, 685
BspMI ACCTGC 1 cut(s) 514
BspT107I GGYRCC 1 cut(s) 638
BspTNI GGTCTC 1 cut(s) 517
BsrI ACTGG 2 cut(s) 450, 842
BssMI GATC 2 cut(s) 252, 432
BssNI GRCGYC 1 cut(s) 740
Bst2UI CCWGG 1 cut(s) 167
Bst4CI ACNGT 4 cut(s) 228, 250, 625, 697
BstACI GRCGYC 1 cut(s) 740
BstC8I GCNNGC 3 cut(s) 176, 208, 710
BstDEI CTNAG 3 cut(s) 38, 60, 606
BstF5I GGATG 3 cut(s) 328, 454, 552
BstKTI GATC 2 cut(s) 255, 435
BstMAI GTCTC 1 cut(s) 517
BstMBI GATC 2 cut(s) 252, 432
BstNI CCWGG 1 cut(s) 167
BstSCI CCNGG 1 cut(s) 165
BstV2I GAAGAC 1 cut(s) 168
BstXI CCANNNNNNTGG 2 cut(s) 376, 395
BsuRI GGCC 1 cut(s) 5
BtgZI GCGATG 1 cut(s) 311
BtsCI GGATG 3 cut(s) 328, 454, 552
BtsIMutI CAGTG 3 cut(s) 262, 487, 756
BveI ACCTGC 1 cut(s) 514
Cac8I GCNNGC 3 cut(s) 176, 208, 710
CaiI CAGNNNCTG 1 cut(s) 848
Cfr13I GGNCC 1 cut(s) 599
CseI GACGC 1 cut(s) 729
CsiI ACCWGGT 1 cut(s) 165
Csp6I GTAC 2 cut(s) 443, 765
CviAII CATG 4 cut(s) 34, 50, 122, 810
CviQI GTAC 2 cut(s) 443, 765
DdeI CTNAG 3 cut(s) 38, 60, 606
DpnI GATC 2 cut(s) 254, 434
DpnII GATC 2 cut(s) 252, 432
DriI GACNNNNNGTC 1 cut(s) 759
EaeI YGGCCR 1 cut(s) 3
Eam1105I GACNNNNNGTC 1 cut(s) 759
EciI GGCGGA 1 cut(s) 468
Eco31I GGTCTC 1 cut(s) 517
Eco47I GGWCC 1 cut(s) 599
Eco57I CTGAAG 2 cut(s) 579, 753
EcoRI GAATTC 1 cut(s) 492
EcoRII CCWGG 1 cut(s) 165
FaeI CATG 4 cut(s) 37, 53, 125, 813
FaqI GGGAC 2 cut(s) 68, 612
FatI CATG 4 cut(s) 33, 49, 121, 809
FbaI TGATCA 2 cut(s) 252, 432
Fnu4HI GCNGC 1 cut(s) 324
FokI GGATG 3 cut(s) 315, 461, 539
Fsp4HI GCNGC 1 cut(s) 324
FspBI CTAG 1 cut(s) 680
GluI GCNGC 1 cut(s) 324
GsaI CCCAGC 3 cut(s) 468, 599, 708
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 835
HgaI GACGC 1 cut(s) 729
Hin1I GRCGYC 1 cut(s) 740
Hin1II CATG 4 cut(s) 37, 53, 125, 813
HinfI GANTC 3 cut(s) 10, 410, 838
HpaII CCGG 1 cut(s) 835
HphI GGTGA 2 cut(s) 181, 739
Hpy166II GTNNAC 1 cut(s) 730
Hpy188I TCNGA 3 cut(s) 214, 351, 609
Hpy188III TCNNGA 5 cut(s) 62, 281, 332, 771, 835
Hpy8I GTNNAC 1 cut(s) 730
Hpy99I CGWCG 1 cut(s) 351
HpyAV CCTTC 5 cut(s) 418, 483, 554, 588, 730
HpyCH4III ACNGT 4 cut(s) 228, 250, 625, 697
HpyCH4IV ACGT 1 cut(s) 828
HpyCH4V TGCA 4 cut(s) 130, 488, 674, 788
HpyF3I CTNAG 3 cut(s) 38, 60, 606
HpySE526I ACGT 1 cut(s) 828
Hsp92I GRCGYC 1 cut(s) 740
Hsp92II CATG 4 cut(s) 37, 53, 125, 813
Kpn2I TCCGGA 1 cut(s) 834
Ksp22I TGATCA 2 cut(s) 252, 432
Kzo9I GATC 2 cut(s) 252, 432
LweI GCATC 1 cut(s) 561
MabI ACCWGGT 1 cut(s) 165
MaeI CTAG 1 cut(s) 680
MaeII ACGT 1 cut(s) 828
MaeIII GTNAC 3 cut(s) 625, 691, 751
MalI GATC 2 cut(s) 254, 434
MboI GATC 2 cut(s) 252, 432
MboII GAAGA 1 cut(s) 173
MhlI GDGCHC 1 cut(s) 539
MluCI AATT 3 cut(s) 100, 399, 492
MlyI GAGTC 2 cut(s) 404, 847
MmeI TCCRAC 1 cut(s) 329
MnlI CCTC 6 cut(s) 130, 277, 510, 652, 718, 767
MroI TCCGGA 1 cut(s) 834
MspI CCGG 1 cut(s) 835
MspR9I CCNGG 1 cut(s) 167
Mva1269I GAATGC 1 cut(s) 788
MvaI CCWGG 1 cut(s) 167
NdeII GATC 2 cut(s) 252, 432
NlaIII CATG 4 cut(s) 37, 53, 125, 813
NlaIV GGNNCC 3 cut(s) 600, 640, 685
NmuCI GTSAC 3 cut(s) 625, 691, 751
PaqCI CACCTGC 1 cut(s) 514
PcsI WCGNNNNNNNCGW 1 cut(s) 343
PctI GAATGC 1 cut(s) 788
PfeI GAWTC 1 cut(s) 10
PkrI GCNGC 1 cut(s) 325
PleI GAGTC 2 cut(s) 404, 846
PpsI GAGTC 2 cut(s) 404, 846
PsiI TTATAA 1 cut(s) 806
Psp6I CCWGG 1 cut(s) 165
PspFI CCCAGC 3 cut(s) 464, 595, 704
PspGI CCWGG 1 cut(s) 165
PspN4I GGNNCC 3 cut(s) 600, 640, 685
PspPI GGNCC 1 cut(s) 599
PstNI CAGNNNCTG 1 cut(s) 848
RsaI GTAC 2 cut(s) 444, 766
RsaNI GTAC 2 cut(s) 443, 765
SatI GCNGC 1 cut(s) 324
Sau3AI GATC 2 cut(s) 252, 432
Sau96I GGNCC 1 cut(s) 599
ScaI AGTACT 1 cut(s) 444
SchI GAGTC 2 cut(s) 404, 847
ScrFI CCNGG 1 cut(s) 167
SduI GDGCHC 1 cut(s) 539
SexAI ACCWGGT 1 cut(s) 165
SfaNI GCATC 1 cut(s) 561
SinI GGWCC 1 cut(s) 599
SmlI CTYRAG 1 cut(s) 279
SmoI CTYRAG 1 cut(s) 279
Sse9I AATT 3 cut(s) 100, 399, 492
SsiI CCGC 2 cut(s) 323, 453
SspMI CTAG 1 cut(s) 680
StyD4I CCNGG 1 cut(s) 165
TaaI ACNGT 4 cut(s) 228, 250, 625, 697
TaiI ACGT 1 cut(s) 831
TaqII GACCGA 1 cut(s) 649
TasI AATT 3 cut(s) 100, 399, 492
TatI WGTACW 1 cut(s) 442
TauI GCSGC 1 cut(s) 326
TfiI GAWTC 1 cut(s) 10
TscAI CASTG 3 cut(s) 262, 487, 756
TseFI GTSAC 3 cut(s) 625, 691, 751
Tsp45I GTSAC 3 cut(s) 625, 691, 751
TspDTI ATGAA 4 cut(s) 22, 323, 465, 570
TspRI CASTG 3 cut(s) 262, 487, 756
VpaK11BI GGWCC 1 cut(s) 599
XapI RAATTY 2 cut(s) 399, 492
XcmI CCANNNNNNNNNTGG 1 cut(s) 510
XspI CTAG 1 cut(s) 680
ZrmI AGTACT 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.