Rh7DG186500

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000302
Physical Location & Seq
Reverse (-)
17317294 .. 17317701
408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG186500.1

Sequence Viewer

Length: 408 bp
ATGGGAAAGAATATGATGGGGAATCGTTATTCCAGGAAAAAAAAATTTAGTTCAAATTTCTTACCTCTTGTTTATGCGGGTGCATATAGCAATGATTCAACAGCTCTGTGCGATTCAGGAACCCTTAATGGAGTTGAAGGGAAAGTGGTGTTGTGTGAAAGAAGTGGAGAAATTAGAAGAATTGCCAGAGGTGAAGAAGTCAAAAGAGCAGGTGGTGCTGCCATGATCCTTATGAACGAAGAAACAAATGGCTATAGCACCTTAGCTGATGGTCATGTGCTTCCCGCAACCCATGTGAGTTATGCTGCAGGTGCAGGTATGAGCATCAAAGCCTACATAAATAAACAGTACAAACTCCTCCTCAACACCTACAACCACAATCTTGTTCAAAGGCACTGTCATCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

135

Amino Acids

14.83

Weight (kDa)

9.57

Isoelectric Point (pI)

39.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PA PF02225 22 - 105 3.9e-13 PA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 200, 299
Acc36I ACCTGC 3 cut(s) 200, 299, 305
AciI CCGC 2 cut(s) 77, 285
AclWI GGATC 1 cut(s) 220
AcsI RAATTY 2 cut(s) 44, 55
AfaI GTAC 1 cut(s) 350
AgsI TTSAA 4 cut(s) 54, 99, 137, 389
AjnI CCWGG 1 cut(s) 32
AluBI AGCT 2 cut(s) 104, 266
AluI AGCT 2 cut(s) 104, 266
AlwI GGATC 1 cut(s) 220
ApeKI GCWGC 2 cut(s) 218, 305
ApoI RAATTY 2 cut(s) 44, 55
ArsI GACNNNNNNTTYG 1 cut(s) 382
AsuHPI GGTGA 1 cut(s) 203
BbvI GCAGC 2 cut(s) 205, 292
BccI CCATC 2 cut(s) 10, 263
BcgI CGANNNNNNTGC 1 cut(s) 383
BciT130I CCWGG 1 cut(s) 34
BfmI CTRYAG 2 cut(s) 253, 306
BfuAI ACCTGC 3 cut(s) 200, 299, 305
BisI GCNGC 2 cut(s) 219, 306
BlsI GCNGC 2 cut(s) 220, 307
Bme1390I CCNGG 1 cut(s) 34
BmiI GGNNCC 1 cut(s) 121
BmrFI CCNGG 1 cut(s) 34
BmsI GCATC 1 cut(s) 333
Bpu10I CCTNAGC 1 cut(s) 262
Bse3DI GCAATG 1 cut(s) 97
BseBI CCWGG 1 cut(s) 34
BseMI GCAATG 1 cut(s) 97
BseRI GAGGAG 2 cut(s) 347, 350
BseXI GCAGC 2 cut(s) 205, 292
BsgI GTGCAG 1 cut(s) 333
Bsp143I GATC 1 cut(s) 225
BspACI CCGC 2 cut(s) 77, 285
BspLI GGNNCC 1 cut(s) 121
BspMAI CTGCAG 1 cut(s) 310
BspMI ACCTGC 3 cut(s) 200, 299, 305
BspPI GGATC 1 cut(s) 220
BsrDI GCAATG 1 cut(s) 97
BssMI GATC 1 cut(s) 225
Bst2UI CCWGG 1 cut(s) 34
Bst4CI ACNGT 2 cut(s) 348, 398
BstAPI GCANNNNNTGC 1 cut(s) 215
BstDEI CTNAG 1 cut(s) 262
BstKTI GATC 1 cut(s) 228
BstMBI GATC 1 cut(s) 225
BstMWI GCNNNNNNNGC 2 cut(s) 215, 311
BstNI CCWGG 1 cut(s) 34
BstSCI CCNGG 1 cut(s) 32
BstSFI CTRYAG 2 cut(s) 253, 306
BstV1I GCAGC 2 cut(s) 205, 292
BtsIMutI CAGTG 1 cut(s) 394
BveI ACCTGC 3 cut(s) 200, 299, 305
Csp6I GTAC 1 cut(s) 349
CviAII CATG 3 cut(s) 223, 275, 293
CviJI RGCY 4 cut(s) 104, 252, 266, 332
CviKI_1 RGCY 4 cut(s) 104, 252, 266, 332
CviQI GTAC 1 cut(s) 349
DdeI CTNAG 1 cut(s) 262
DpnI GATC 1 cut(s) 227
DpnII GATC 1 cut(s) 225
EcoRII CCWGG 1 cut(s) 32
FaeI CATG 3 cut(s) 226, 278, 296
FatI CATG 3 cut(s) 222, 274, 292
FauI CCCGC 2 cut(s) 70, 292
Fnu4HI GCNGC 2 cut(s) 219, 306
Fsp4HI GCNGC 2 cut(s) 219, 306
GluI GCNGC 2 cut(s) 219, 306
Hin1II CATG 3 cut(s) 226, 278, 296
HinfI GANTC 3 cut(s) 22, 95, 113
HphI GGTGA 1 cut(s) 203
Hpy188III TCNNGA 1 cut(s) 117
HpyAV CCTTC 1 cut(s) 131
HpyCH4III ACNGT 2 cut(s) 348, 398
HpyCH4V TGCA 3 cut(s) 83, 308, 314
HpyF10VI GCNNNNNNNGC 2 cut(s) 215, 311
HpyF3I CTNAG 1 cut(s) 262
Hsp92II CATG 3 cut(s) 226, 278, 296
Kzo9I GATC 1 cut(s) 225
LpnPI CCDG 7 cut(s) 19, 46, 102, 195, 199, 294, 300
Lsp1109I GCAGC 2 cut(s) 205, 292
LweI GCATC 1 cut(s) 333
MalI GATC 1 cut(s) 227
MboI GATC 1 cut(s) 225
MboII GAAGA 3 cut(s) 189, 206, 251
MluCI AATT 4 cut(s) 44, 55, 171, 180
MnlI CCTC 4 cut(s) 75, 182, 368, 371
MseI TTAA 1 cut(s) 126
MspR9I CCNGG 1 cut(s) 34
MvaI CCWGG 1 cut(s) 34
MwoI GCNNNNNNNGC 2 cut(s) 215, 311
NdeII GATC 1 cut(s) 225
NlaIII CATG 3 cut(s) 226, 278, 296
NlaIV GGNNCC 1 cut(s) 121
PaqCI CACCTGC 2 cut(s) 200, 299
PfeI GAWTC 3 cut(s) 22, 95, 113
PfoI TCCNGGA 1 cut(s) 32
PkrI GCNGC 2 cut(s) 220, 307
Psp6I CCWGG 1 cut(s) 32
PspGI CCWGG 1 cut(s) 32
PspN4I GGNNCC 1 cut(s) 121
PstI CTGCAG 1 cut(s) 310
RsaI GTAC 1 cut(s) 350
RsaNI GTAC 1 cut(s) 349
SaqAI TTAA 1 cut(s) 126
SatI GCNGC 2 cut(s) 219, 306
Sau3AI GATC 1 cut(s) 225
ScrFI CCNGG 1 cut(s) 34
SetI ASST 9 cut(s) 67, 106, 193, 214, 263, 268, 313, 319, 371
SfaNI GCATC 1 cut(s) 333
SfcI CTRYAG 2 cut(s) 253, 306
Sse9I AATT 4 cut(s) 44, 55, 171, 180
SsiI CCGC 2 cut(s) 77, 285
StyD4I CCNGG 1 cut(s) 32
TaaI ACNGT 2 cut(s) 348, 398
TasI AATT 4 cut(s) 44, 55, 171, 180
TatI WGTACW 1 cut(s) 348
TfiI GAWTC 3 cut(s) 22, 95, 113
Tru1I TTAA 1 cut(s) 126
Tru9I TTAA 1 cut(s) 126
TscAI CASTG 1 cut(s) 401
TseI GCWGC 2 cut(s) 218, 305
TspDTI ATGAA 1 cut(s) 248
TspRI CASTG 1 cut(s) 401
XapI RAATTY 2 cut(s) 44, 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.