Rh7AG183400

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000302
Physical Location & Seq
Reverse (-)
16307422 .. 16309843
2422 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG183400.1

Sequence Viewer

Length: 927 bp
ATGAAGCAACGAATGGTTTATGCATACCACCATGTGGTGACTGGCATTGTAGCAAAGCTGACGGTAGAGGAAGTAAAAGAAATGGAGACAAAAGATGGGTTTATGTCCGCTCATCTAGATAGGATTCTGCCTTTGCACACCACCCATAGTCCGAACTTCTTGAGGTTATACCAAGGATTGGGACTTTGGAAAGAGTCTAATTATGGTGAAGGTGTAATTATTCGAGTTTTCGACTCTGGGACAACACTAGGTCATCCCTCATTCAGCGATGAAGGAGTGCCACCTCCTCCGGCTAAATGGAAAGGTAAGTGTGATTTCAACGGGACATTCTGCAATAACAAGCTTATTGGTGCCAGAAGTTTCCGATGTGGGCAAACTACTGGAGATCCACCAATTGATGTTGATGGCCATGGAACCCTTAATGGAGTTGAAGGGAAAGTGGTGCTGTGTGAAAGAAGTGGAGAAATTGGAAGAATTGCCAGAGGTGAAGAAGTCAAAAGAGCAGGTGGTGCTGCCATGATCCTTATGAACGAAGAAACAAATGGCTATAGCACCTTAGCTGATGCTCATGTGCTTCCCGCAACCCATGTGAGTTATGCTGCAGGTGTAGGTACCTCAATGTCATGCCCTCACCTGAGTGGCATTGCAGCTTTGCTCAAGAGCTCTCACCCTGATTGGTCACCAGCTGCCATTAAGTCTGCAATTGTGACAACTGCTCAAGTACTAAACCTCGGCGTCAAGCCCATTGTGGATGAAACACTTGGTCCTGCTGACATCTTTGCCACTGGTGCAGGCCATGTTAACCCTTCAAGAGAAGATGACCGCGGGCTAATCTTTGACTTAGAACCAGCGGATTACATCCCCTATCTATGCGGTTTGAATTACAGTGACGATCAGATACAGATCATCACCCAACAAACAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

308

Amino Acids

33.17

Weight (kDa)

5.62

Isoelectric Point (pI)

30.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PA PF02225 142 - 202 1.1e-10 PA domain
Peptidase_S8 PF00082 188 - 242 4.1e-12 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 494, 593
Acc36I ACCTGC 2 cut(s) 494, 593
Acc65I GGTACC 1 cut(s) 611
AccB1I GGYRCC 2 cut(s) 350, 611
AccB7I CCANNNNNTGG 2 cut(s) 34, 178
AccBSI CCGCTC 1 cut(s) 110
AccII CGCG 1 cut(s) 825
AciI CCGC 6 cut(s) 108, 579, 823, 825, 851, 873
AclWI GGATC 2 cut(s) 380, 514
AcoI YGGCCR 1 cut(s) 406
AcyI GRCGYC 1 cut(s) 735
AdeI CACNNNGTG 1 cut(s) 34
AfaI GTAC 2 cut(s) 613, 723
AfiI CCNNNNNNNGG 2 cut(s) 34, 178
AgsI TTSAA 4 cut(s) 319, 431, 810, 880
AleI CACNNNNGTG 1 cut(s) 636
AluBI AGCT 6 cut(s) 58, 343, 560, 650, 663, 686
AluI AGCT 6 cut(s) 58, 343, 560, 650, 663, 686
Alw21I GWGCWC 1 cut(s) 665
Alw26I GTCTC 1 cut(s) 80
AlwI GGATC 2 cut(s) 380, 514
AoxI GGCC 2 cut(s) 406, 793
ApeKI GCWGC 4 cut(s) 512, 599, 647, 686
Asp718I GGTACC 1 cut(s) 611
AspS9I GGNCC 1 cut(s) 764
AsuHPI GGTGA 7 cut(s) 49, 218, 497, 623, 659, 672, 901
AvaII GGWCC 1 cut(s) 764
BalI TGGCCA 1 cut(s) 408
BanI GGYRCC 2 cut(s) 350, 611
BanII GRGCYC 1 cut(s) 665
Bbv12I GWGCWC 1 cut(s) 665
BbvI GCAGC 4 cut(s) 499, 586, 659, 673
BccI CCATC 2 cut(s) 89, 398
BcoDI GTCTC 1 cut(s) 80
BfaI CTAG 2 cut(s) 116, 248
BfmI CTRYAG 2 cut(s) 547, 600
BfuAI ACCTGC 2 cut(s) 494, 593
BisI GCNGC 4 cut(s) 513, 600, 648, 687
BlsI GCNGC 4 cut(s) 514, 601, 649, 688
BmcAI AGTACT 1 cut(s) 723
Bme18I GGWCC 1 cut(s) 764
BmgT120I GGNCC 1 cut(s) 764
BmiI GGNNCC 3 cut(s) 352, 415, 613
BmsI GCATC 1 cut(s) 553
BpmI CTGGAG 1 cut(s) 402
Bpu10I CCTNAGC 1 cut(s) 556
BpuEI CTTGAG 3 cut(s) 181, 641, 702
BsaBI GATNNNNATC 1 cut(s) 902
BsaHI GRCGYC 1 cut(s) 735
BsaJI CCNNGG 4 cut(s) 172, 409, 730, 823
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 178
Bse1I ACTGG 3 cut(s) 46, 385, 790
Bse3DI GCAATG 1 cut(s) 642
Bse8I GATNNNNATC 1 cut(s) 902
BseDI CCNNGG 4 cut(s) 172, 409, 730, 823
BseGI GGATG 3 cut(s) 253, 757, 858
BseJI GATNNNNATC 1 cut(s) 902
BseLI CCNNNNNNNGG 2 cut(s) 34, 178
BseMI GCAATG 1 cut(s) 642
BseMII CTCAG 1 cut(s) 626
BseNI ACTGG 3 cut(s) 46, 385, 790
BseRI GAGGAG 1 cut(s) 276
BseXI GCAGC 4 cut(s) 499, 586, 659, 673
BsgI GTGCAG 1 cut(s) 810
Bsh1236I CGCG 1 cut(s) 825
BshFI GGCC 2 cut(s) 408, 795
BshNI GGYRCC 2 cut(s) 350, 611
BsiHKAI GWGCWC 1 cut(s) 665
BsiSI CCGG 1 cut(s) 290
BslFI GGGAC 3 cut(s) 195, 253, 337
BslI CCNNNNNNNGG 2 cut(s) 34, 178
BsmAI GTCTC 1 cut(s) 80
BsmFI GGGAC 3 cut(s) 195, 253, 337
BsnI GGCC 2 cut(s) 408, 795
Bsp1286I GDGCHC 1 cut(s) 665
Bsp143I GATC 4 cut(s) 385, 519, 892, 903
Bsp19I CCATGG 1 cut(s) 409
BspACI CCGC 6 cut(s) 108, 579, 823, 825, 851, 873
BspANI GGCC 2 cut(s) 408, 795
BspCNI CTCAG 1 cut(s) 627
BspFNI CGCG 1 cut(s) 825
BspLI GGNNCC 3 cut(s) 352, 415, 613
BspMAI CTGCAG 1 cut(s) 604
BspMI ACCTGC 2 cut(s) 494, 593
BspPI GGATC 2 cut(s) 380, 514
BspT107I GGYRCC 2 cut(s) 350, 611
BsrBI CCGCTC 1 cut(s) 110
BsrDI GCAATG 1 cut(s) 642
BsrI ACTGG 3 cut(s) 46, 385, 790
BssECI CCNNGG 4 cut(s) 172, 409, 730, 823
BssMI GATC 4 cut(s) 385, 519, 892, 903
BssNI GRCGYC 1 cut(s) 735
BssT1I CCWWGG 2 cut(s) 172, 409
Bst4CI ACNGT 2 cut(s) 64, 887
BstACI GRCGYC 1 cut(s) 735
BstAPI GCANNNNNTGC 1 cut(s) 509
BstC8I GCNNGC 2 cut(s) 793, 827
BstDEI CTNAG 3 cut(s) 556, 635, 841
BstDSI CCRYGG 2 cut(s) 409, 823
BstEII GGTNACC 1 cut(s) 678
BstF5I GGATG 3 cut(s) 253, 757, 858
BstFNI CGCG 1 cut(s) 825
BstKTI GATC 4 cut(s) 388, 522, 895, 906
BstMAI GTCTC 1 cut(s) 80
BstMBI GATC 4 cut(s) 385, 519, 892, 903
BstMWI GCNNNNNNNGC 2 cut(s) 509, 788
BstPI GGTNACC 1 cut(s) 678
BstSFI CTRYAG 2 cut(s) 547, 600
BstUI CGCG 1 cut(s) 825
BstV1I GCAGC 4 cut(s) 499, 586, 659, 673
BstX2I RGATCY 1 cut(s) 385
BstYI RGATCY 1 cut(s) 385
BsuRI GGCC 2 cut(s) 408, 795
BtgI CCRYGG 2 cut(s) 409, 823
BtgZI GCGATG 1 cut(s) 282
BtsCI GGATG 3 cut(s) 253, 757, 858
BtsIMutI CAGTG 2 cut(s) 783, 892
BveI ACCTGC 2 cut(s) 494, 593
Cac8I GCNNGC 2 cut(s) 793, 827
Cfr13I GGNCC 1 cut(s) 764
Cfr42I CCGCGG 1 cut(s) 826
CseI GACGC 1 cut(s) 724
Csp6I GTAC 2 cut(s) 612, 722
CviAII CATG 7 cut(s) 32, 410, 517, 569, 587, 624, 797
CviQI GTAC 2 cut(s) 612, 722
DdeI CTNAG 3 cut(s) 556, 635, 841
DpnI GATC 4 cut(s) 387, 521, 894, 905
DpnII GATC 4 cut(s) 385, 519, 892, 903
DraIII CACNNNGTG 1 cut(s) 34
EaeI YGGCCR 1 cut(s) 406
Ecl136II GAGCTC 1 cut(s) 663
Eco130I CCWWGG 2 cut(s) 172, 409
Eco24I GRGCYC 1 cut(s) 665
Eco47I GGWCC 1 cut(s) 764
Eco53kI GAGCTC 1 cut(s) 663
Eco91I GGTNACC 1 cut(s) 678
EcoICRI GAGCTC 1 cut(s) 663
EcoO65I GGTNACC 1 cut(s) 678
EcoT14I CCWWGG 2 cut(s) 172, 409
EcoT22I ATGCAT 1 cut(s) 25
EcoT38I GRGCYC 1 cut(s) 665
ErhI CCWWGG 2 cut(s) 172, 409
FaeI CATG 7 cut(s) 35, 413, 520, 572, 590, 627, 800
FaqI GGGAC 3 cut(s) 195, 253, 337
FatI CATG 7 cut(s) 31, 409, 516, 568, 586, 623, 796
FauI CCCGC 2 cut(s) 586, 818
Fnu4HI GCNGC 4 cut(s) 513, 600, 648, 687
FokI GGATG 3 cut(s) 240, 764, 845
FriOI GRGCYC 1 cut(s) 665
Fsp4HI GCNGC 4 cut(s) 513, 600, 648, 687
FspBI CTAG 2 cut(s) 116, 248
GluI GCNGC 4 cut(s) 513, 600, 648, 687
GsuI CTGGAG 1 cut(s) 402
HaeIII GGCC 2 cut(s) 408, 795
HapII CCGG 1 cut(s) 290
HgaI GACGC 1 cut(s) 724
Hin1I GRCGYC 1 cut(s) 735
Hin1II CATG 7 cut(s) 35, 413, 520, 572, 590, 627, 800
HincII GTYRAC 1 cut(s) 802
HindII GTYRAC 1 cut(s) 802
HindIII AAGCTT 1 cut(s) 341
HinfI GANTC 3 cut(s) 124, 194, 233
HpaI GTTAAC 1 cut(s) 802
HpaII CCGG 1 cut(s) 290
HphI GGTGA 7 cut(s) 49, 218, 497, 623, 659, 672, 901
Hpy166II GTNNAC 1 cut(s) 802
Hpy188I TCNGA 3 cut(s) 153, 365, 897
Hpy188III TCNNGA 4 cut(s) 116, 160, 658, 810
Hpy8I GTNNAC 1 cut(s) 802
HpyAV CCTTC 4 cut(s) 203, 266, 425, 816
HpyCH4III ACNGT 2 cut(s) 64, 887
HpyCH4V TGCA 7 cut(s) 23, 136, 333, 602, 647, 701, 791
HpyF10VI GCNNNNNNNGC 2 cut(s) 509, 788
HpyF3I CTNAG 3 cut(s) 556, 635, 841
Hsp92I GRCGYC 1 cut(s) 735
Hsp92II CATG 7 cut(s) 35, 413, 520, 572, 590, 627, 800
KpnI GGTACC 1 cut(s) 615
KspAI GTTAAC 1 cut(s) 802
KspI CCGCGG 1 cut(s) 826
Kzo9I GATC 4 cut(s) 385, 519, 892, 903
Lsp1109I GCAGC 4 cut(s) 499, 586, 659, 673
LweI GCATC 1 cut(s) 553
MaeI CTAG 2 cut(s) 116, 248
MaeIII GTNAC 4 cut(s) 37, 678, 706, 887
MalI GATC 4 cut(s) 387, 521, 894, 905
MbiI CCGCTC 1 cut(s) 110
MboI GATC 4 cut(s) 385, 519, 892, 903
MboII GAAGA 4 cut(s) 483, 500, 545, 827
MfeI CAATTG 2 cut(s) 393, 702
MflI RGATCY 1 cut(s) 385
MhlI GDGCHC 1 cut(s) 665
MlsI TGGCCA 1 cut(s) 408
MluCI AATT 7 cut(s) 199, 216, 393, 465, 474, 702, 880
MluNI TGGCCA 1 cut(s) 408
MlyI GAGTC 2 cut(s) 203, 227
MnlI CCTC 9 cut(s) 61, 156, 268, 294, 297, 476, 625, 639, 740
Mox20I TGGCCA 1 cut(s) 408
Mph1103I ATGCAT 1 cut(s) 25
MscI TGGCCA 1 cut(s) 408
MseI TTAA 3 cut(s) 420, 693, 801
MslI CAYNNNNRTG 1 cut(s) 636
Msp20I TGGCCA 1 cut(s) 408
MspA1I CMGCKG 3 cut(s) 686, 825, 851
MspI CCGG 1 cut(s) 290
MunI CAATTG 2 cut(s) 393, 702
MvnI CGCG 1 cut(s) 825
MwoI GCNNNNNNNGC 2 cut(s) 509, 788
NcoI CCATGG 1 cut(s) 409
NdeII GATC 4 cut(s) 385, 519, 892, 903
NlaIII CATG 7 cut(s) 35, 413, 520, 572, 590, 627, 800
NlaIV GGNNCC 3 cut(s) 352, 415, 613
NmeAIII GCCGAG 1 cut(s) 711
NmuCI GTSAC 4 cut(s) 37, 678, 706, 887
NsiI ATGCAT 1 cut(s) 25
OliI CACNNNNGTG 1 cut(s) 636
PaqCI CACCTGC 2 cut(s) 494, 593
PfeI GAWTC 1 cut(s) 124
PflMI CCANNNNNTGG 2 cut(s) 34, 178
PkrI GCNGC 4 cut(s) 514, 601, 649, 688
PleI GAGTC 2 cut(s) 202, 227
PpsI GAGTC 2 cut(s) 202, 227
Psp124BI GAGCTC 1 cut(s) 665
PspEI GGTNACC 1 cut(s) 678
PspN4I GGNNCC 3 cut(s) 352, 415, 613
PspPI GGNCC 1 cut(s) 764
PstI CTGCAG 1 cut(s) 604
PsuI RGATCY 1 cut(s) 385
PvuII CAGCTG 1 cut(s) 686
RsaI GTAC 2 cut(s) 613, 723
RsaNI GTAC 2 cut(s) 612, 722
RseI CAYNNNNRTG 1 cut(s) 636
SacI GAGCTC 1 cut(s) 665
SacII CCGCGG 1 cut(s) 826
SaqAI TTAA 3 cut(s) 420, 693, 801
SatI GCNGC 4 cut(s) 513, 600, 648, 687
Sau3AI GATC 4 cut(s) 385, 519, 892, 903
Sau96I GGNCC 1 cut(s) 764
ScaI AGTACT 1 cut(s) 723
SchI GAGTC 2 cut(s) 203, 227
SduI GDGCHC 1 cut(s) 665
SfaNI GCATC 1 cut(s) 553
SfcI CTRYAG 2 cut(s) 547, 600
Sfr303I CCGCGG 1 cut(s) 826
SgrBI CCGCGG 1 cut(s) 826
SinI GGWCC 1 cut(s) 764
SmiMI CAYNNNNRTG 1 cut(s) 636
SmlI CTYRAG 3 cut(s) 160, 656, 717
SmoI CTYRAG 3 cut(s) 160, 656, 717
Sse9I AATT 7 cut(s) 199, 216, 393, 465, 474, 702, 880
SsiI CCGC 6 cut(s) 108, 579, 823, 825, 851, 873
SspMI CTAG 2 cut(s) 116, 248
SstI GAGCTC 1 cut(s) 665
StyI CCWWGG 2 cut(s) 172, 409
TaaI ACNGT 2 cut(s) 64, 887
TaqI TCGA 2 cut(s) 223, 231
TasI AATT 7 cut(s) 199, 216, 393, 465, 474, 702, 880
TatI WGTACW 1 cut(s) 721
TfiI GAWTC 1 cut(s) 124
Tru1I TTAA 3 cut(s) 420, 693, 801
Tru9I TTAA 3 cut(s) 420, 693, 801
TscAI CASTG 2 cut(s) 790, 892
TseFI GTSAC 4 cut(s) 37, 678, 706, 887
TseI GCWGC 4 cut(s) 512, 599, 647, 686
Tsp45I GTSAC 4 cut(s) 37, 678, 706, 887
TspDTI ATGAA 4 cut(s) 17, 285, 542, 768
TspRI CASTG 2 cut(s) 790, 892
Van91I CCANNNNNTGG 2 cut(s) 34, 178
VpaK11BI GGWCC 1 cut(s) 764
XbaI TCTAGA 1 cut(s) 115
XcmI CCANNNNNNNNNTGG 1 cut(s) 38
XspI CTAG 2 cut(s) 116, 248
ZrmI AGTACT 1 cut(s) 723
Zsp2I ATGCAT 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.