subtilisin-like protease
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 314 |
| Acc36I | ACCTGC | 2 cut(s) | 314, 974 |
| AciI | CCGC | 3 cut(s) | 1082, 1224, 1344 |
| AclWI | GGATC | 1 cut(s) | 1166 |
| AcsI | RAATTY | 1 cut(s) | 117 |
| AcuI | CTGAAG | 2 cut(s) | 930, 1134 |
| AcvI | CACGTG | 1 cut(s) | 523 |
| AfaI | GTAC | 4 cut(s) | 805, 1037, 1196, 1328 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1435 |
| AgsI | TTSAA | 5 cut(s) | 300, 383, 616, 703, 810 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 268 |
| AjnI | CCWGG | 2 cut(s) | 691, 1003 |
| AleI | CACNNNNGTG | 1 cut(s) | 828 |
| Alw21I | GWGCWC | 3 cut(s) | 331, 857, 1296 |
| Alw26I | GTCTC | 3 cut(s) | 658, 725, 1408 |
| Alw44I | GTGCAC | 1 cut(s) | 327 |
| AlwI | GGATC | 1 cut(s) | 1166 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 932 |
| AoxI | GGCC | 3 cut(s) | 177, 746, 1219 |
| ApaLI | GTGCAC | 1 cut(s) | 327 |
| ApeKI | GCWGC | 4 cut(s) | 740, 878, 959, 1358 |
| ApoI | RAATTY | 1 cut(s) | 117 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 609, 641 |
| AspS9I | GGNCC | 5 cut(s) | 149, 178, 366, 674, 1220 |
| AsuHPI | GGTGA | 7 cut(s) | 571, 644, 815, 864, 1081, 1219, 1450 |
| AvaII | GGWCC | 3 cut(s) | 149, 366, 674 |
| AxyI | CCTNAGG | 1 cut(s) | 1287 |
| BaeGI | GKGCMC | 1 cut(s) | 331 |
| BanII | GRGCYC | 3 cut(s) | 857, 1011, 1296 |
| BbrPI | CACGTG | 1 cut(s) | 523 |
| Bbv12I | GWGCWC | 3 cut(s) | 331, 857, 1296 |
| BbvI | GCAGC | 4 cut(s) | 727, 865, 971, 1345 |
| BccI | CCATC | 4 cut(s) | 14, 71, 902, 1361 |
| BceAI | ACGGC | 1 cut(s) | 959 |
| BciT130I | CCWGG | 2 cut(s) | 693, 1005 |
| BclI | TGATCA | 1 cut(s) | 634 |
| BcoDI | GTCTC | 3 cut(s) | 658, 725, 1408 |
| BfaI | CTAG | 2 cut(s) | 407, 737 |
| BfmI | CTRYAG | 3 cut(s) | 9, 552, 597 |
| BfuAI | ACCTGC | 2 cut(s) | 314, 974 |
| BisI | GCNGC | 4 cut(s) | 741, 879, 960, 1359 |
| BlpI | GCTNAGC | 1 cut(s) | 58 |
| BlsI | GCNGC | 4 cut(s) | 742, 880, 961, 1360 |
| Bme1390I | CCNGG | 2 cut(s) | 693, 1005 |
| Bme18I | GGWCC | 3 cut(s) | 149, 366, 674 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 268 |
| BmgT120I | GGNCC | 5 cut(s) | 149, 178, 366, 674, 1220 |
| BmiI | GGNNCC | 6 cut(s) | 150, 180, 367, 368, 1123, 1222 |
| BmrFI | CCNGG | 2 cut(s) | 693, 1005 |
| BmsI | GCATC | 3 cut(s) | 349, 573, 741 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 58 |
| BpuEI | CTTGAG | 1 cut(s) | 833 |
| BsaAI | YACGTR | 1 cut(s) | 523 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1271 |
| BsaI | GGTCTC | 3 cut(s) | 658, 725, 1408 |
| BsaJI | CCNNGG | 3 cut(s) | 182, 1003, 1004 |
| BsaWI | WCCGGW | 1 cut(s) | 800 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 577, 607 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1435 |
| Bse1I | ACTGG | 1 cut(s) | 872 |
| Bse21I | CCTNAGG | 1 cut(s) | 1287 |
| Bse3DI | GCAATG | 1 cut(s) | 343 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1271 |
| BseBI | CCWGG | 2 cut(s) | 693, 1005 |
| BseDI | CCNNGG | 3 cut(s) | 182, 1003, 1004 |
| BseGI | GGATG | 4 cut(s) | 34, 732, 949, 1372 |
| BseJI | GATNNNNATC | 1 cut(s) | 1271 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1435 |
| BseMI | GCAATG | 1 cut(s) | 343 |
| BseMII | CTCAG | 5 cut(s) | 72, 1202, 1278, 1385, 1397 |
| BseNI | ACTGG | 1 cut(s) | 872 |
| BseRI | GAGGAG | 2 cut(s) | 1046, 1304 |
| BseSI | GKGCMC | 1 cut(s) | 331 |
| BseXI | GCAGC | 4 cut(s) | 727, 865, 971, 1345 |
| BseYI | CCCAGC | 3 cut(s) | 137, 1178, 1398 |
| BsgI | GTGCAG | 1 cut(s) | 1002 |
| BshFI | GGCC | 3 cut(s) | 179, 748, 1221 |
| BsiHKAI | GWGCWC | 3 cut(s) | 331, 857, 1296 |
| BsiSI | CCGG | 1 cut(s) | 801 |
| BsiWI | CGTACG | 1 cut(s) | 1326 |
| BslFI | GGGAC | 2 cut(s) | 162, 352 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1435 |
| BsmAI | GTCTC | 3 cut(s) | 658, 725, 1408 |
| BsmFI | GGGAC | 2 cut(s) | 162, 352 |
| BsmI | GAATGC | 1 cut(s) | 1414 |
| BsnI | GGCC | 3 cut(s) | 179, 748, 1221 |
| Bso31I | GGTCTC | 3 cut(s) | 658, 725, 1408 |
| Bsp1286I | GDGCHC | 4 cut(s) | 331, 857, 1011, 1296 |
| Bsp143I | GATC | 3 cut(s) | 634, 1171, 1440 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 58 |
| Bsp19I | CCATGG | 1 cut(s) | 182 |
| BspACI | CCGC | 3 cut(s) | 1082, 1224, 1344 |
| BspANI | GGCC | 3 cut(s) | 179, 748, 1221 |
| BspCNI | CTCAG | 5 cut(s) | 71, 1201, 1279, 1386, 1396 |
| BspHI | TCATGA | 2 cut(s) | 897, 988 |
| BspLI | GGNNCC | 6 cut(s) | 150, 180, 367, 368, 1123, 1222 |
| BspMAI | CTGCAG | 2 cut(s) | 13, 556 |
| BspMI | ACCTGC | 2 cut(s) | 314, 974 |
| BspPI | GGATC | 1 cut(s) | 1166 |
| BspTNI | GGTCTC | 3 cut(s) | 658, 725, 1408 |
| BsrDI | GCAATG | 1 cut(s) | 343 |
| BsrI | ACTGG | 1 cut(s) | 872 |
| BssECI | CCNNGG | 3 cut(s) | 182, 1003, 1004 |
| BssMI | GATC | 3 cut(s) | 634, 1171, 1440 |
| BssT1I | CCWWGG | 1 cut(s) | 182 |
| Bst2UI | CCWGG | 2 cut(s) | 693, 1005 |
| Bst4CI | ACNGT | 4 cut(s) | 196, 306, 625, 1207 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 92, 932 |
| BstBAI | YACGTR | 1 cut(s) | 523 |
| BstC8I | GCNNGC | 5 cut(s) | 205, 496, 642, 928, 1412 |
| BstDEI | CTNAG | 7 cut(s) | 58, 827, 1188, 1287, 1383, 1394, 1452 |
| BstDSI | CCRYGG | 1 cut(s) | 182 |
| BstEII | GGTNACC | 1 cut(s) | 870 |
| BstF5I | GGATG | 4 cut(s) | 34, 732, 949, 1372 |
| BstKTI | GATC | 3 cut(s) | 637, 1174, 1443 |
| BstMAI | GTCTC | 3 cut(s) | 658, 725, 1408 |
| BstMBI | GATC | 3 cut(s) | 634, 1171, 1440 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 92, 101, 932, 980 |
| BstNI | CCWGG | 2 cut(s) | 693, 1005 |
| BstNSI | RCATGY | 1 cut(s) | 542 |
| BstPI | GGTNACC | 1 cut(s) | 870 |
| BstSCI | CCNGG | 2 cut(s) | 691, 1003 |
| BstSFI | CTRYAG | 3 cut(s) | 9, 552, 597 |
| BstSLI | GKGCMC | 1 cut(s) | 331 |
| BstV1I | GCAGC | 4 cut(s) | 727, 865, 971, 1345 |
| BstX2I | RGATCY | 1 cut(s) | 1171 |
| BstYI | RGATCY | 1 cut(s) | 1171 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1287 |
| BsuRI | GGCC | 3 cut(s) | 179, 748, 1221 |
| BtgI | CCRYGG | 1 cut(s) | 182 |
| BtgZI | GCGATG | 1 cut(s) | 372 |
| BtsCI | GGATG | 4 cut(s) | 34, 732, 949, 1372 |
| BtsI | GCAGTG | 1 cut(s) | 6 |
| BtsIMutI | CAGTG | 3 cut(s) | 6, 621, 1282 |
| BveI | ACCTGC | 2 cut(s) | 314, 974 |
| Cac8I | GCNNGC | 5 cut(s) | 205, 496, 642, 928, 1412 |
| CaiI | CAGNNNCTG | 1 cut(s) | 932 |
| CciI | TCATGA | 2 cut(s) | 897, 988 |
| Cfr13I | GGNCC | 5 cut(s) | 149, 178, 366, 674, 1220 |
| CseI | GACGC | 1 cut(s) | 524 |
| Csp6I | GTAC | 4 cut(s) | 804, 1036, 1195, 1327 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 420, 455 |
| CviAII | CATG | 7 cut(s) | 183, 469, 539, 744, 816, 898, 989 |
| CviQI | GTAC | 4 cut(s) | 804, 1036, 1195, 1327 |
| DdeI | CTNAG | 7 cut(s) | 58, 827, 1188, 1287, 1383, 1394, 1452 |
| DpnI | GATC | 3 cut(s) | 636, 1173, 1442 |
| DpnII | GATC | 3 cut(s) | 634, 1171, 1440 |
| DraI | TTTAAA | 1 cut(s) | 1059 |
| DriI | GACNNNNNGTC | 1 cut(s) | 268 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 268 |
| Ecl136II | GAGCTC | 2 cut(s) | 855, 1294 |
| Eco130I | CCWWGG | 1 cut(s) | 182 |
| Eco24I | GRGCYC | 3 cut(s) | 857, 1011, 1296 |
| Eco31I | GGTCTC | 3 cut(s) | 658, 725, 1408 |
| Eco32I | GATATC | 1 cut(s) | 1391 |
| Eco47I | GGWCC | 3 cut(s) | 149, 366, 674 |
| Eco53kI | GAGCTC | 2 cut(s) | 855, 1294 |
| Eco57I | CTGAAG | 2 cut(s) | 930, 1134 |
| Eco72I | CACGTG | 1 cut(s) | 523 |
| Eco81I | CCTNAGG | 1 cut(s) | 1287 |
| Eco91I | GGTNACC | 1 cut(s) | 870 |
| EcoICRI | GAGCTC | 2 cut(s) | 855, 1294 |
| EcoO109I | RGGNCCY | 3 cut(s) | 149, 178, 366 |
| EcoO65I | GGTNACC | 1 cut(s) | 870 |
| EcoRII | CCWGG | 2 cut(s) | 691, 1003 |
| EcoRV | GATATC | 1 cut(s) | 1391 |
| EcoT14I | CCWWGG | 1 cut(s) | 182 |
| EcoT38I | GRGCYC | 3 cut(s) | 857, 1011, 1296 |
| ErhI | CCWWGG | 1 cut(s) | 182 |
| FaeI | CATG | 7 cut(s) | 186, 472, 542, 747, 819, 901, 992 |
| FaqI | GGGAC | 2 cut(s) | 162, 352 |
| FatI | CATG | 7 cut(s) | 182, 468, 538, 743, 815, 897, 988 |
| FauI | CCCGC | 1 cut(s) | 1231 |
| FbaI | TGATCA | 1 cut(s) | 634 |
| Fnu4HI | GCNGC | 4 cut(s) | 741, 879, 960, 1359 |
| FokI | GGATG | 4 cut(s) | 41, 719, 956, 1379 |
| FriOI | GRGCYC | 3 cut(s) | 857, 1011, 1296 |
| Fsp4HI | GCNGC | 4 cut(s) | 741, 879, 960, 1359 |
| FspBI | CTAG | 2 cut(s) | 407, 737 |
| GluI | GCNGC | 4 cut(s) | 741, 879, 960, 1359 |
| GsaI | CCCAGC | 3 cut(s) | 141, 1182, 1402 |
| HaeIII | GGCC | 3 cut(s) | 179, 748, 1221 |
| HapII | CCGG | 1 cut(s) | 801 |
| HgaI | GACGC | 1 cut(s) | 524 |
| Hin1II | CATG | 7 cut(s) | 186, 472, 542, 747, 819, 901, 992 |
| HincII | GTYRAC | 1 cut(s) | 481 |
| HindII | GTYRAC | 1 cut(s) | 481 |
| HindIII | AAGCTT | 1 cut(s) | 239 |
| HinfI | GANTC | 5 cut(s) | 187, 269, 341, 472, 773 |
| HpaII | CCGG | 1 cut(s) | 801 |
| HphI | GGTGA | 7 cut(s) | 571, 644, 815, 864, 1081, 1219, 1450 |
| Hpy166II | GTNNAC | 7 cut(s) | 329, 481, 760, 916, 1197, 1259, 1341 |
| Hpy188I | TCNGA | 5 cut(s) | 531, 648, 1114, 1191, 1395 |
| Hpy188III | TCNNGA | 4 cut(s) | 669, 850, 898, 989 |
| Hpy8I | GTNNAC | 7 cut(s) | 329, 481, 760, 916, 1197, 1259, 1341 |
| HpyAV | CCTTC | 4 cut(s) | 106, 377, 643, 1357 |
| HpyCH4III | ACNGT | 4 cut(s) | 196, 306, 625, 1207 |
| HpyCH4IV | ACGT | 2 cut(s) | 522, 1325 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 92, 101, 932, 980 |
| HpyF3I | CTNAG | 7 cut(s) | 58, 827, 1188, 1287, 1383, 1394, 1452 |
| HpySE526I | ACGT | 2 cut(s) | 522, 1325 |
| Hsp92II | CATG | 7 cut(s) | 186, 472, 542, 747, 819, 901, 992 |
| KflI | GGGWCCC | 1 cut(s) | 366 |
| Ksp22I | TGATCA | 1 cut(s) | 634 |
| Kzo9I | GATC | 3 cut(s) | 634, 1171, 1440 |
| LmnI | GCTCC | 5 cut(s) | 101, 200, 649, 1121, 1291 |
| Lsp1109I | GCAGC | 4 cut(s) | 727, 865, 971, 1345 |
| LweI | GCATC | 3 cut(s) | 349, 573, 741 |
| MaeI | CTAG | 2 cut(s) | 407, 737 |
| MaeII | ACGT | 2 cut(s) | 522, 1325 |
| MaeIII | GTNAC | 4 cut(s) | 306, 545, 870, 1207 |
| MalI | GATC | 3 cut(s) | 636, 1173, 1442 |
| MboI | GATC | 3 cut(s) | 634, 1171, 1440 |
| MboII | GAAGA | 1 cut(s) | 435 |
| MfeI | CAATTG | 1 cut(s) | 996 |
| MflI | RGATCY | 1 cut(s) | 1171 |
| MhlI | GDGCHC | 4 cut(s) | 331, 857, 1011, 1296 |
| MluCI | AATT | 9 cut(s) | 81, 105, 117, 417, 426, 996, 1097, 1144, 1241 |
| MlyI | GAGTC | 1 cut(s) | 278 |
| MmeI | TCCRAC | 2 cut(s) | 178, 554 |
| MseI | TTAA | 6 cut(s) | 108, 372, 789, 885, 1058, 1311 |
| MslI | CAYNNNNRTG | 3 cut(s) | 336, 828, 1277 |
| MspA1I | CMGCKG | 2 cut(s) | 129, 878 |
| MspI | CCGG | 1 cut(s) | 801 |
| MspR9I | CCNGG | 2 cut(s) | 693, 1005 |
| MssI | GTTTAAAC | 1 cut(s) | 1059 |
| MunI | CAATTG | 1 cut(s) | 996 |
| Mva1269I | GAATGC | 1 cut(s) | 1414 |
| MvaI | CCWGG | 2 cut(s) | 693, 1005 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 92, 101, 932, 980 |
| NcoI | CCATGG | 1 cut(s) | 182 |
| NdeII | GATC | 3 cut(s) | 634, 1171, 1440 |
| NlaIII | CATG | 7 cut(s) | 186, 472, 542, 747, 819, 901, 992 |
| NlaIV | GGNNCC | 6 cut(s) | 150, 180, 367, 368, 1123, 1222 |
| NmuCI | GTSAC | 2 cut(s) | 870, 1207 |
| NspI | RCATGY | 1 cut(s) | 542 |
| OliI | CACNNNNGTG | 1 cut(s) | 828 |
| PagI | TCATGA | 2 cut(s) | 897, 988 |
| PaqCI | CACCTGC | 1 cut(s) | 314 |
| PasI | CCCWGGG | 1 cut(s) | 1004 |
| PctI | GAATGC | 1 cut(s) | 1414 |
| PfeI | GAWTC | 4 cut(s) | 187, 341, 472, 773 |
| Pfl23II | CGTACG | 1 cut(s) | 1326 |
| PkrI | GCNGC | 4 cut(s) | 742, 880, 961, 1360 |
| PleI | GAGTC | 1 cut(s) | 277 |
| PmaCI | CACGTG | 1 cut(s) | 523 |
| PmeI | GTTTAAAC | 1 cut(s) | 1059 |
| PmlI | CACGTG | 1 cut(s) | 523 |
| PpsI | GAGTC | 1 cut(s) | 277 |
| Ppu21I | YACGTR | 1 cut(s) | 523 |
| PpuMI | RGGWCCY | 2 cut(s) | 149, 366 |
| Psp124BI | GAGCTC | 2 cut(s) | 857, 1296 |
| Psp5II | RGGWCCY | 2 cut(s) | 149, 366 |
| Psp6I | CCWGG | 2 cut(s) | 691, 1003 |
| PspCI | CACGTG | 1 cut(s) | 523 |
| PspEI | GGTNACC | 1 cut(s) | 870 |
| PspFI | CCCAGC | 3 cut(s) | 137, 1178, 1398 |
| PspGI | CCWGG | 2 cut(s) | 691, 1003 |
| PspLI | CGTACG | 1 cut(s) | 1326 |
| PspN4I | GGNNCC | 6 cut(s) | 150, 180, 367, 368, 1123, 1222 |
| PspPI | GGNCC | 5 cut(s) | 149, 178, 366, 674, 1220 |
| PspPPI | RGGWCCY | 2 cut(s) | 149, 366 |
| PstI | CTGCAG | 2 cut(s) | 13, 556 |
| PstNI | CAGNNNCTG | 1 cut(s) | 932 |
| PsuI | RGATCY | 1 cut(s) | 1171 |
| PvuII | CAGCTG | 2 cut(s) | 129, 878 |
| RsaI | GTAC | 4 cut(s) | 805, 1037, 1196, 1328 |
| RsaNI | GTAC | 4 cut(s) | 804, 1036, 1195, 1327 |
| RseI | CAYNNNNRTG | 3 cut(s) | 336, 828, 1277 |
| SacI | GAGCTC | 2 cut(s) | 857, 1296 |
| SaqAI | TTAA | 6 cut(s) | 108, 372, 789, 885, 1058, 1311 |
| SatI | GCNGC | 4 cut(s) | 741, 879, 960, 1359 |
| Sau3AI | GATC | 3 cut(s) | 634, 1171, 1440 |
| Sau96I | GGNCC | 5 cut(s) | 149, 178, 366, 674, 1220 |
| SchI | GAGTC | 1 cut(s) | 278 |
| ScrFI | CCNGG | 2 cut(s) | 693, 1005 |
| SduI | GDGCHC | 4 cut(s) | 331, 857, 1011, 1296 |
| SfaNI | GCATC | 3 cut(s) | 349, 573, 741 |
| SfcI | CTRYAG | 3 cut(s) | 9, 552, 597 |
| SinI | GGWCC | 3 cut(s) | 149, 366, 674 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 336, 828, 1277 |
| SmlI | CTYRAG | 1 cut(s) | 848 |
| SmoI | CTYRAG | 1 cut(s) | 848 |
| Sse9I | AATT | 9 cut(s) | 81, 105, 117, 417, 426, 996, 1097, 1144, 1241 |
| SsiI | CCGC | 3 cut(s) | 1082, 1224, 1344 |
| SspMI | CTAG | 2 cut(s) | 407, 737 |
| SstI | GAGCTC | 2 cut(s) | 857, 1296 |
| StyD4I | CCNGG | 2 cut(s) | 691, 1003 |
| StyI | CCWWGG | 1 cut(s) | 182 |
| TaaI | ACNGT | 4 cut(s) | 196, 306, 625, 1207 |
| TaiI | ACGT | 2 cut(s) | 525, 1328 |
| TaqI | TCGA | 1 cut(s) | 668 |
| TasI | AATT | 9 cut(s) | 81, 105, 117, 417, 426, 996, 1097, 1144, 1241 |
| TatI | WGTACW | 2 cut(s) | 1035, 1194 |
| TfiI | GAWTC | 4 cut(s) | 187, 341, 472, 773 |
| Tru1I | TTAA | 6 cut(s) | 108, 372, 789, 885, 1058, 1311 |
| Tru9I | TTAA | 6 cut(s) | 108, 372, 789, 885, 1058, 1311 |
| TscAI | CASTG | 3 cut(s) | 13, 628, 1282 |
| TseFI | GTSAC | 2 cut(s) | 870, 1207 |
| TseI | GCWGC | 4 cut(s) | 740, 878, 959, 1358 |
| Tsp45I | GTSAC | 2 cut(s) | 870, 1207 |
| TspDTI | ATGAA | 5 cut(s) | 333, 654, 960, 1005, 1254 |
| TspRI | CASTG | 3 cut(s) | 13, 628, 1282 |
| VneI | GTGCAC | 1 cut(s) | 327 |
| VpaK11BI | GGWCC | 3 cut(s) | 149, 366, 674 |
| XapI | RAATTY | 1 cut(s) | 117 |
| XceI | RCATGY | 1 cut(s) | 542 |
| XspI | CTAG | 2 cut(s) | 407, 737 |