MD04G1072200.v1.1

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000302
Physical Location & Seq
Reverse (-)
9843423 .. 9845309
1887 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1072200.v1.1.491

Sequence Viewer

Length: 1887 bp
ATGTTATACCTTCTCGGTTTTACATTCATGTTTTTTTTGTCAGTAGCAGTTGCACAAGAGAAAAATGGCATGCAAACTTATATTGTTTTCGTAGAAAAGCCAGTTTCACAGAAGTTTTCTGATCAATTGGATTTGGAGAGTTGGTATCAGTCATTTTTGCCTGAAACAGCTCAAAGCTCAAACCAAGAGACAAGTTCGAGAATCGTTCATGCATACCGCAAAGTGGTAACTGGCTTTGCAGCAAAACTGACACCAGAAGAAGTGAAAGCAATGAAAAACAAAGAAGGGTTCGTGTCAGCTCGCGAGGAGAGAATTCTACCTTTGCAAACCACTCATAGTCCTGACTTCTTGGGATTACACCAAGGATTCGGACTATGGAAACAAACAAACTACGGTGAAGGTGTGATCATAGGACTTTTGGATACAGGAATTGGACCTGATCATCCTTCATTCAGCGACGAAGGAGTATCCCCTCCTCCTGCTAAATGGAAAGGCAAGTGCGAATTCAATGGCACAGTCTGCAACAACAAGCTTATTGGTGCACGAAATTTCATAGGTGCAGAAGAAGGGCATATTACAGGAACTCCATTTGATAATCACGGCCATGGTACCCATACTTCTAGCACTGCTGCTGGAAATTTTGTGCAAGGAGCCAGCGTGTTCGGAGAGGCCAATGGTACAGCTGTTGGAATGGCACCTTATGCTCACTTGGCAATGTACAAAGTCTGCACTGAAACTGGTTGTGCTGATGGTGACATTTTAGCTGCTCTTGATGTCGCTGTTGAAGATGGCGTGGATGTACTCTCCCTCTCACTTGGTGGTGAATCACTTCCTTTCTACAATGATGTGCTTGCAATTGGTGCATTTGCAGCAATTCAAAAAGGAATTTTTTTCAGCTGTGCAGCTGGAAATTCTGGTCCTTCCTACGAGTCTTTGTCAAATGAGGCCCCATGGATTCTCACAGTGGGAGCAAGCACCACTGACAGAATACTAAGATTGGAAGGTGAGAAGCTCAACATATACAACACTGTAATTGGAGATGCACTTGCTCCCAAGGTTGCTTTCTTTTCTTCAAGAGGACCAAGCATTGCTAGCCCCGGAATTCTAAAGCCAGATATCATTGGACCGGGTGTTGACATCCTAGCAGCATGGCCTGAATCAGTGGACAATGCCACACTTCCTAATCCCAAGGCAACATTTAACATAATTTCGGGTACCTCAATGGCAACTCCTCACCTAAGTGGCATTGGAGCTCTACTCAAGAAATCACACCCTGATTGGTCTCCGGCTGCTATTAAATCTGCCATTATGACAACAGCTAATGTACTGAACCTCGCAGGCTCCCCCATTGTTGATCAACAACTTCAACCTGCAGACATTTTTACTCTTGGTGCAGGCCATGTCAATCCTGCAAGGGCAAATGACCCAGGGCTCATCTTTGACATAAAACCTGAGGATTACATTCCTTACTTGTGTGGTTTATATCACAATGAAACACAGATAAAGATGATCACCCAACGAGTAGTGAACTGCTCTCAAGCCGGAGCCATACCAGAAGCACAGCTTAATTATCCTTCATTTGCTATTACAATAGGTTCAAATGAGACTCAATCTCAGTATTATACAAGGACTGTGAGGAATGTTGGTCCGGCTAGTTCAACTTACAGCTTGGATCTTTTAGTACCACATAAAATGGGGATGAGTGTGAATCCTCAGGTGCTTACATTCACAAAGGTTAATCAGGAGATTACATACCATGTTGAATTTATCGCAGAAGATGGGGCTGGGAAGGATGATGTACCATTTGGTCAGGGTTATTTGAGATGGGTTTCTGATAAGCACAATGTTACTACCCCCATAGCAGTGGTGTTTACTCAACAACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

629

Amino Acids

67.4

Weight (kDa)

5.33

Isoelectric Point (pI)

35.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 26 - 108 8.4e-11 Peptidase inhibitor I9
Peptidase_S8 PF00082 132 - 442 1.9e-46 Subtilase family
fn3_6 PF17766 521 - 622 1.1e-24 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1378
Acc65I GGTACC 2 cut(s) 608, 1214
AccB1I GGYRCC 3 cut(s) 608, 694, 1214
AccII CGCG 1 cut(s) 303
AciI CCGC 1 cut(s) 217
AclWI GGATC 1 cut(s) 1680
AcoI YGGCCR 1 cut(s) 601
AcsI RAATTY 8 cut(s) 312, 503, 547, 637, 885, 910, 1101, 1763
AdeI CACNNNGTG 1 cut(s) 818
AfaI GTAC 8 cut(s) 610, 679, 719, 801, 1216, 1326, 1683, 1800
AfiI CCNNNNNNNGG 1 cut(s) 223
AgsI TTSAA 8 cut(s) 508, 785, 878, 1074, 1367, 1599, 1659, 1763
AjnI CCWGG 1 cut(s) 1426
AjuI GAANNNNNNNTTGG 2 cut(s) 1046, 1078
AleI CACNNNNGTG 1 cut(s) 1239
Alw21I GWGCWC 2 cut(s) 544, 1255
Alw26I GTCTC 3 cut(s) 182, 1287, 1598
Alw44I GTGCAC 1 cut(s) 540
AlwI GGATC 1 cut(s) 1680
AoxI GGCC 5 cut(s) 601, 669, 945, 1151, 1396
ApaLI GTGCAC 1 cut(s) 540
ApeKI GCWGC 7 cut(s) 239, 629, 764, 869, 902, 1145, 1289
ApoI RAATTY 8 cut(s) 312, 503, 547, 637, 885, 910, 1101, 1763
Asp700I GAANNNNTTC 1 cut(s) 828
Asp718I GGTACC 2 cut(s) 608, 1214
AspS9I GGNCC 6 cut(s) 434, 917, 946, 1079, 1124, 1646
AsuC2I CCSGG 2 cut(s) 1098, 1128
AsuHPI GGTGA 6 cut(s) 407, 764, 833, 1016, 1226, 1504
AsuNHI GCTAGC 1 cut(s) 1091
AvaII GGWCC 5 cut(s) 434, 917, 1079, 1124, 1646
AxyI CCTNAGG 2 cut(s) 1452, 1713
BaeGI GKGCMC 1 cut(s) 544
BaeI ACNNNNGTAYC 2 cut(s) 669, 702
BanI GGYRCC 3 cut(s) 608, 694, 1214
BanII GRGCYC 2 cut(s) 1255, 1434
BarI GAAGNNNNNNTAC 2 cut(s) 601, 633
Bbv12I GWGCWC 2 cut(s) 544, 1255
BbvI GCAGC 7 cut(s) 251, 616, 751, 881, 914, 1157, 1276
BccI CCATC 4 cut(s) 743, 782, 1772, 1818
BceAI ACGGC 1 cut(s) 616
BciT130I CCWGG 1 cut(s) 1428
BciVI GTATCC 2 cut(s) 415, 478
BclI TGATCA 5 cut(s) 121, 405, 439, 1354, 1509
BcnI CCSGG 2 cut(s) 1098, 1128
BcoDI GTCTC 3 cut(s) 182, 1287, 1598
BfaI CTAG 4 cut(s) 621, 1092, 1142, 1653
BfmI CTRYAG 1 cut(s) 1371
BfuAI ACCTGC 1 cut(s) 1378
BfuI GTATCC 2 cut(s) 415, 478
BisI GCNGC 7 cut(s) 240, 630, 765, 870, 903, 1146, 1290
BlsI GCNGC 7 cut(s) 241, 631, 766, 871, 904, 1147, 1291
Bme1390I CCNGG 3 cut(s) 1098, 1128, 1428
Bme18I GGWCC 5 cut(s) 434, 917, 1079, 1124, 1646
BmgT120I GGNCC 6 cut(s) 434, 917, 946, 1079, 1124, 1646
BmiI GGNNCC 7 cut(s) 610, 652, 696, 948, 1216, 1342, 1546
BmrFI CCNGG 3 cut(s) 1098, 1128, 1428
BmsI GCATC 1 cut(s) 1030
BmtI GCTAGC 1 cut(s) 1095
BplI GAGNNNNNCTC 2 cut(s) 1242, 1274
BpuEI CTTGAG 2 cut(s) 1244, 1521
BpuMI CCSGG 2 cut(s) 1098, 1128
BsaI GGTCTC 1 cut(s) 1287
BsaJI CCNNGG 8 cut(s) 361, 604, 950, 1053, 1096, 1188, 1426, 1427
BsaXI ACNNNNNCTCC 6 cut(s) 128, 158, 568, 598, 642, 672
Bsc4I CCNNNNNNNGG 1 cut(s) 223
Bse1I ACTGG 3 cut(s) 101, 235, 742
Bse21I CCTNAGG 2 cut(s) 1452, 1713
Bse3DI GCAATG 3 cut(s) 276, 720, 1086
BseBI CCWGG 1 cut(s) 1428
BseDI CCNNGG 8 cut(s) 361, 604, 950, 1053, 1096, 1188, 1426, 1427
BseGI GGATG 5 cut(s) 442, 802, 1137, 1704, 1798
BseLI CCNNNNNNNGG 1 cut(s) 223
BseMI GCAATG 3 cut(s) 276, 720, 1086
BseMII CTCAG 3 cut(s) 1443, 1628, 1727
BseNI ACTGG 3 cut(s) 101, 235, 742
BseRI GAGGAG 3 cut(s) 320, 465, 1221
BseSI GKGCMC 1 cut(s) 544
BseXI GCAGC 7 cut(s) 251, 616, 751, 881, 914, 1157, 1276
BseYI CCCAGC 1 cut(s) 1784
BsgI GTGCAG 4 cut(s) 579, 712, 921, 1413
Bsh1236I CGCG 1 cut(s) 303
BshFI GGCC 5 cut(s) 603, 671, 947, 1153, 1398
BshNI GGYRCC 3 cut(s) 608, 694, 1214
BsiHKAI GWGCWC 2 cut(s) 544, 1255
BsiSI CCGG 5 cut(s) 1098, 1127, 1286, 1542, 1649
BslI CCNNNNNNNGG 1 cut(s) 223
BsmAI GTCTC 3 cut(s) 182, 1287, 1598
BsnI GGCC 5 cut(s) 603, 671, 947, 1153, 1398
Bso31I GGTCTC 1 cut(s) 1287
Bsp1286I GDGCHC 3 cut(s) 544, 1255, 1434
Bsp1407I TGTACA 1 cut(s) 717
Bsp143I GATC 6 cut(s) 121, 405, 439, 1354, 1509, 1672
Bsp19I CCATGG 2 cut(s) 604, 950
Bsp68I TCGCGA 1 cut(s) 303
BspACI CCGC 1 cut(s) 217
BspANI GGCC 5 cut(s) 603, 671, 947, 1153, 1398
BspCNI CTCAG 3 cut(s) 1444, 1627, 1726
BspFNI CGCG 1 cut(s) 303
BspLI GGNNCC 7 cut(s) 610, 652, 696, 948, 1216, 1342, 1546
BspMAI CTGCAG 1 cut(s) 1375
BspMI ACCTGC 1 cut(s) 1378
BspOI GCTAGC 1 cut(s) 1095
BspPI GGATC 1 cut(s) 1680
BspT107I GGYRCC 3 cut(s) 608, 694, 1214
BspTNI GGTCTC 1 cut(s) 1287
BsrDI GCAATG 3 cut(s) 276, 720, 1086
BsrGI TGTACA 1 cut(s) 717
BsrI ACTGG 3 cut(s) 101, 235, 742
BssECI CCNNGG 8 cut(s) 361, 604, 950, 1053, 1096, 1188, 1426, 1427
BssMI GATC 6 cut(s) 121, 405, 439, 1354, 1509, 1672
BssT1I CCWWGG 5 cut(s) 361, 604, 950, 1053, 1188
Bst2UI CCWGG 1 cut(s) 1428
Bst4CI ACNGT 5 cut(s) 395, 517, 964, 1030, 1633
BstAPI GCANNNNNTGC 3 cut(s) 519, 701, 860
BstAUI TGTACA 1 cut(s) 717
BstC8I GCNNGC 8 cut(s) 71, 301, 655, 852, 973, 1093, 1339, 1396
BstDEI CTNAG 5 cut(s) 992, 1238, 1452, 1614, 1713
BstDSI CCRYGG 2 cut(s) 604, 950
BstF5I GGATG 5 cut(s) 442, 802, 1137, 1704, 1798
BstFNI CGCG 1 cut(s) 303
BstKTI GATC 6 cut(s) 124, 408, 442, 1357, 1512, 1675
BstMAI GTCTC 3 cut(s) 182, 1287, 1598
BstMBI GATC 6 cut(s) 121, 405, 439, 1354, 1509, 1672
BstMWI GCNNNNNNNGC 6 cut(s) 519, 701, 710, 860, 869, 1092
BstNI CCWGG 1 cut(s) 1428
BstNSI RCATGY 1 cut(s) 73
BstSCI CCNGG 3 cut(s) 1096, 1126, 1426
BstSFI CTRYAG 1 cut(s) 1371
BstSLI GKGCMC 1 cut(s) 544
BstUI CGCG 1 cut(s) 303
BstV1I GCAGC 7 cut(s) 251, 616, 751, 881, 914, 1157, 1276
BstX2I RGATCY 1 cut(s) 1672
BstXI CCANNNNNNTGG 1 cut(s) 1864
BstYI RGATCY 1 cut(s) 1672
Bsu36I CCTNAGG 2 cut(s) 1452, 1713
BsuI GTATCC 2 cut(s) 415, 478
BsuRI GGCC 5 cut(s) 603, 671, 947, 1153, 1398
BtgI CCRYGG 2 cut(s) 604, 950
BtsCI GGATG 5 cut(s) 442, 802, 1137, 1704, 1798
BtsI GCAGTG 2 cut(s) 624, 1869
BtsIMutI CAGTG 7 cut(s) 624, 729, 969, 978, 1026, 1167, 1869
BtuMI TCGCGA 1 cut(s) 303
BveI ACCTGC 1 cut(s) 1378
Cac8I GCNNGC 8 cut(s) 71, 301, 655, 852, 973, 1093, 1339, 1396
Cfr13I GGNCC 6 cut(s) 434, 917, 946, 1079, 1124, 1646
Csp6I GTAC 8 cut(s) 609, 678, 718, 800, 1215, 1325, 1682, 1799
CviAII CATG 8 cut(s) 28, 70, 209, 605, 951, 1149, 1400, 1757
CviQI GTAC 8 cut(s) 609, 678, 718, 800, 1215, 1325, 1682, 1799
DdeI CTNAG 5 cut(s) 992, 1238, 1452, 1614, 1713
DpnI GATC 6 cut(s) 123, 407, 441, 1356, 1511, 1674
DpnII GATC 6 cut(s) 121, 405, 439, 1354, 1509, 1672
DraIII CACNNNGTG 1 cut(s) 818
EaeI YGGCCR 1 cut(s) 601
Ecl136II GAGCTC 1 cut(s) 1253
Eco130I CCWWGG 5 cut(s) 361, 604, 950, 1053, 1188
Eco24I GRGCYC 2 cut(s) 1255, 1434
Eco31I GGTCTC 1 cut(s) 1287
Eco32I GATATC 1 cut(s) 1117
Eco47I GGWCC 5 cut(s) 434, 917, 1079, 1124, 1646
Eco53kI GAGCTC 1 cut(s) 1253
Eco81I CCTNAGG 2 cut(s) 1452, 1713
EcoICRI GAGCTC 1 cut(s) 1253
EcoO109I RGGNCCY 1 cut(s) 946
EcoRI GAATTC 3 cut(s) 312, 503, 1101
EcoRII CCWGG 1 cut(s) 1426
EcoRV GATATC 1 cut(s) 1117
EcoT14I CCWWGG 5 cut(s) 361, 604, 950, 1053, 1188
EcoT22I ATGCAT 1 cut(s) 214
EcoT38I GRGCYC 2 cut(s) 1255, 1434
ErhI CCWWGG 5 cut(s) 361, 604, 950, 1053, 1188
FaeI CATG 8 cut(s) 31, 73, 212, 608, 954, 1152, 1403, 1760
FalI AAGNNNNNCTT 2 cut(s) 1548, 1580
FatI CATG 8 cut(s) 27, 69, 208, 604, 950, 1148, 1399, 1756
FbaI TGATCA 5 cut(s) 121, 405, 439, 1354, 1509
Fnu4HI GCNGC 7 cut(s) 240, 630, 765, 870, 903, 1146, 1290
FokI GGATG 5 cut(s) 429, 809, 1124, 1711, 1805
FriOI GRGCYC 2 cut(s) 1255, 1434
Fsp4HI GCNGC 7 cut(s) 240, 630, 765, 870, 903, 1146, 1290
FspBI CTAG 4 cut(s) 621, 1092, 1142, 1653
GluI GCNGC 7 cut(s) 240, 630, 765, 870, 903, 1146, 1290
GsaI CCCAGC 1 cut(s) 1788
HaeIII GGCC 5 cut(s) 603, 671, 947, 1153, 1398
HapII CCGG 5 cut(s) 1098, 1127, 1286, 1542, 1649
Hin1II CATG 8 cut(s) 31, 73, 212, 608, 954, 1152, 1403, 1760
HincII GTYRAC 1 cut(s) 1135
HindII GTYRAC 1 cut(s) 1135
HindIII AAGCTT 1 cut(s) 530
HinfI GANTC 8 cut(s) 201, 366, 824, 929, 955, 1157, 1606, 1708
HpaII CCGG 5 cut(s) 1098, 1127, 1286, 1542, 1649
HphI GGTGA 6 cut(s) 407, 764, 833, 1016, 1226, 1504
Hpy166II GTNNAC 5 cut(s) 542, 1135, 1165, 1528, 1872
Hpy188I TCNGA 4 cut(s) 121, 371, 665, 1834
Hpy188III TCNNGA 7 cut(s) 198, 302, 341, 770, 1074, 1261, 1742
Hpy8I GTNNAC 5 cut(s) 542, 1135, 1165, 1528, 1872
Hpy99I CGWCG 1 cut(s) 461
HpyCH4III ACNGT 5 cut(s) 395, 517, 964, 1030, 1633
HpyF10VI GCNNNNNNNGC 6 cut(s) 519, 701, 710, 860, 869, 1092
HpyF3I CTNAG 5 cut(s) 992, 1238, 1452, 1614, 1713
Hsp92II CATG 8 cut(s) 31, 73, 212, 608, 954, 1152, 1403, 1760
KpnI GGTACC 2 cut(s) 612, 1218
Ksp22I TGATCA 5 cut(s) 121, 405, 439, 1354, 1509
Kzo9I GATC 6 cut(s) 121, 405, 439, 1354, 1509, 1672
LmnI GCTCC 6 cut(s) 650, 968, 1054, 1250, 1346, 1544
Lsp1109I GCAGC 7 cut(s) 251, 616, 751, 881, 914, 1157, 1276
LweI GCATC 1 cut(s) 1030
MaeI CTAG 4 cut(s) 621, 1092, 1142, 1653
MaeIII GTNAC 3 cut(s) 226, 752, 1846
MalI GATC 6 cut(s) 123, 407, 441, 1356, 1511, 1674
MboI GATC 6 cut(s) 121, 405, 439, 1354, 1509, 1672
MboII GAAGA 5 cut(s) 269, 575, 797, 1062, 1787
MfeI CAATTG 2 cut(s) 125, 855
MflI RGATCY 1 cut(s) 1672
MhlI GDGCHC 3 cut(s) 544, 1255, 1434
MlyI GAGTC 2 cut(s) 938, 1600
MmeI TCCRAC 1 cut(s) 667
Mph1103I ATGCAT 1 cut(s) 214
MroXI GAANNNNTTC 1 cut(s) 828
MseI TTAA 4 cut(s) 1200, 1296, 1566, 1737
MslI CAYNNNNRTG 3 cut(s) 603, 1239, 1862
MspA1I CMGCKG 3 cut(s) 683, 897, 905
MspI CCGG 5 cut(s) 1098, 1127, 1286, 1542, 1649
MspR9I CCNGG 3 cut(s) 1098, 1128, 1428
MunI CAATTG 2 cut(s) 125, 855
MvaI CCWGG 1 cut(s) 1428
MvnI CGCG 1 cut(s) 303
MwoI GCNNNNNNNGC 6 cut(s) 519, 701, 710, 860, 869, 1092
NciI CCSGG 2 cut(s) 1098, 1128
NcoI CCATGG 2 cut(s) 604, 950
NdeII GATC 6 cut(s) 121, 405, 439, 1354, 1509, 1672
NheI GCTAGC 1 cut(s) 1091
NlaIII CATG 8 cut(s) 31, 73, 212, 608, 954, 1152, 1403, 1760
NlaIV GGNNCC 7 cut(s) 610, 652, 696, 948, 1216, 1342, 1546
NmuCI GTSAC 1 cut(s) 752
NruI TCGCGA 1 cut(s) 303
NsiI ATGCAT 1 cut(s) 214
NspI RCATGY 1 cut(s) 73
OliI CACNNNNGTG 1 cut(s) 1239
PaeI GCATGC 1 cut(s) 73
PasI CCCWGGG 1 cut(s) 1427
PdmI GAANNNNTTC 1 cut(s) 828
PfeI GAWTC 6 cut(s) 201, 366, 824, 955, 1157, 1708
PkrI GCNGC 7 cut(s) 241, 631, 766, 871, 904, 1147, 1291
PleI GAGTC 2 cut(s) 937, 1600
PpsI GAGTC 2 cut(s) 937, 1600
Psp124BI GAGCTC 1 cut(s) 1255
Psp6I CCWGG 1 cut(s) 1426
PspFI CCCAGC 1 cut(s) 1784
PspGI CCWGG 1 cut(s) 1426
PspN4I GGNNCC 7 cut(s) 610, 652, 696, 948, 1216, 1342, 1546
PspPI GGNCC 6 cut(s) 434, 917, 946, 1079, 1124, 1646
PstI CTGCAG 1 cut(s) 1375
PsuI RGATCY 1 cut(s) 1672
PvuII CAGCTG 3 cut(s) 683, 897, 905
RruI TCGCGA 1 cut(s) 303
RsaI GTAC 8 cut(s) 610, 679, 719, 801, 1216, 1326, 1683, 1800
RsaNI GTAC 8 cut(s) 609, 678, 718, 800, 1215, 1325, 1682, 1799
RseI CAYNNNNRTG 3 cut(s) 603, 1239, 1862
SacI GAGCTC 1 cut(s) 1255
SaqAI TTAA 4 cut(s) 1200, 1296, 1566, 1737
SatI GCNGC 7 cut(s) 240, 630, 765, 870, 903, 1146, 1290
Sau3AI GATC 6 cut(s) 121, 405, 439, 1354, 1509, 1672
Sau96I GGNCC 6 cut(s) 434, 917, 946, 1079, 1124, 1646
SchI GAGTC 2 cut(s) 938, 1600
ScrFI CCNGG 3 cut(s) 1098, 1128, 1428
SduI GDGCHC 3 cut(s) 544, 1255, 1434
SfaNI GCATC 1 cut(s) 1030
SfcI CTRYAG 1 cut(s) 1371
SinI GGWCC 5 cut(s) 434, 917, 1079, 1124, 1646
SmiMI CAYNNNNRTG 3 cut(s) 603, 1239, 1862
SmlI CTYRAG 2 cut(s) 1259, 1536
SmoI CTYRAG 2 cut(s) 1259, 1536
SphI GCATGC 1 cut(s) 73
SsiI CCGC 1 cut(s) 217
SspMI CTAG 4 cut(s) 621, 1092, 1142, 1653
SstI GAGCTC 1 cut(s) 1255
StyD4I CCNGG 3 cut(s) 1096, 1126, 1426
StyI CCWWGG 5 cut(s) 361, 604, 950, 1053, 1188
TaaI ACNGT 5 cut(s) 395, 517, 964, 1030, 1633
TaqI TCGA 1 cut(s) 197
TatI WGTACW 3 cut(s) 717, 799, 1324
TfiI GAWTC 6 cut(s) 201, 366, 824, 955, 1157, 1708
Tru1I TTAA 4 cut(s) 1200, 1296, 1566, 1737
Tru9I TTAA 4 cut(s) 1200, 1296, 1566, 1737
TscAI CASTG 7 cut(s) 631, 736, 969, 985, 1033, 1167, 1869
TseFI GTSAC 1 cut(s) 752
TseI GCWGC 7 cut(s) 239, 629, 764, 869, 902, 1145, 1289
Tsp45I GTSAC 1 cut(s) 752
TspDTI ATGAA 7 cut(s) 16, 197, 287, 438, 541, 1506, 1566
TspRI CASTG 7 cut(s) 631, 736, 969, 985, 1033, 1167, 1869
VneI GTGCAC 1 cut(s) 540
VpaK11BI GGWCC 5 cut(s) 434, 917, 1079, 1124, 1646
XapI RAATTY 8 cut(s) 312, 503, 547, 637, 885, 910, 1101, 1763
XceI RCATGY 1 cut(s) 73
XmnI GAANNNNTTC 1 cut(s) 828
XspI CTAG 4 cut(s) 621, 1092, 1142, 1653
Zsp2I ATGCAT 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.