MD04G1071900.v1.1

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000302
Physical Location & Seq
Reverse (-)
9756542 .. 9757210
669 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1071900.v1.1.491

Sequence Viewer

Length: 669 bp
ATGGCAACTCCTCACCTAAGTGGCATTGCAGCTTTGATCAAGAAATCACACCCTGACTGGTCTCCAGCTGCAATCAAATCTGCCATCATGACAACCGCTGATGTACTAAATCTTGCAGGCACACCGATCGTGGATCAAGATCTTACACCCGCGGATGTTTTCGCAATTGGTGGAGGCCATGTCAACCCTGCAAAGGCAAATGATCCGGGGCTTATCTTCGACATAAAACCTGAGGATTACTTTCCTTACTTGTGTGGATTGAATTACAATGAGACAGCAATAAAGATCATCACACAACAAACAGTGAAATGCTCTGAAGTCGGGGTCATACTAGAAGCACAGCTCAATTACCCTTCATTTTCTATTAAAGCAGGCCCGAACCAGACTCAGTCTCAGTATTATACACGGACTGCGAAGAACGTCGGCCCCGCTACGTCAACTTACAACTTGGATCTTTTAGTACCACATCAAATGGGAATGAGTGTGAATCCTCAGGTGCTTACATTCACAGAGGTTAACCAGGAGATTACATACCATGTCGAATTTATCGCAGAAGATGGTGCTGGGAAGGATGGCGTTCCATACGGTCAAGGATACTTGAGATGGTTTTCTGATAAGCATAATGTTACTACCCCTATAGCTGTCATCTTTGACACCCCGCAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

223

Amino Acids

24.1

Weight (kDa)

4.98

Isoelectric Point (pI)

32.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 1 - 35 1e-07 Subtilase family
fn3_6 PF17766 114 - 215 1.3e-23 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 152
AciI CCGC 5 cut(s) 96, 150, 152, 429, 659
AclWI GGATC 3 cut(s) 141, 197, 459
AcsI RAATTY 1 cut(s) 542
AcuI CTGAAG 1 cut(s) 336
AfaI GTAC 2 cut(s) 105, 462
AfiI CCNNNNNNNGG 1 cut(s) 193
AgsI TTSAA 1 cut(s) 262
AjnI CCWGG 1 cut(s) 519
AleI CACNNNNGTG 1 cut(s) 18
AluBI AGCT 4 cut(s) 32, 68, 343, 641
AluI AGCT 4 cut(s) 32, 68, 343, 641
Alw26I GTCTC 3 cut(s) 66, 266, 396
AlwI GGATC 3 cut(s) 141, 197, 459
AoxI GGCC 3 cut(s) 175, 373, 424
ApeKI GCWGC 2 cut(s) 29, 68
ApoI RAATTY 1 cut(s) 542
AspS9I GGNCC 2 cut(s) 374, 425
AsuC2I CCSGG 1 cut(s) 207
AsuHPI GGTGA 1 cut(s) 5
AxyI CCTNAGG 2 cut(s) 231, 492
BbvI GCAGC 2 cut(s) 41, 55
BccI CCATC 4 cut(s) 92, 551, 566, 597
BcgI CGANNNNNNTGC 2 cut(s) 109, 143
BciT130I CCWGG 1 cut(s) 521
BciVI GTATCC 1 cut(s) 587
BclI TGATCA 1 cut(s) 36
BcnI CCSGG 1 cut(s) 207
BcoDI GTCTC 3 cut(s) 66, 266, 396
BfaI CTAG 1 cut(s) 332
BfmI CTRYAG 1 cut(s) 636
BfuI GTATCC 1 cut(s) 587
BglII AGATCT 1 cut(s) 139
BisI GCNGC 2 cut(s) 30, 69
BlsI GCNGC 2 cut(s) 31, 70
Bme1390I CCNGG 2 cut(s) 207, 521
BmgT120I GGNCC 2 cut(s) 374, 425
BmiI GGNNCC 1 cut(s) 427
BmrFI CCNGG 2 cut(s) 207, 521
BpmI CTGGAG 1 cut(s) 48
BpuEI CTTGAG 1 cut(s) 619
BpuMI CCSGG 1 cut(s) 207
BsaBI GATNNNNATC 1 cut(s) 138
BsaI GGTCTC 1 cut(s) 66
BsaJI CCNNGG 2 cut(s) 150, 206
BsaXI ACNNNNNCTCC 2 cut(s) 165, 195
Bsc4I CCNNNNNNNGG 1 cut(s) 193
Bse1I ACTGG 1 cut(s) 62
Bse21I CCTNAGG 2 cut(s) 231, 492
Bse3DI GCAATG 1 cut(s) 24
Bse8I GATNNNNATC 1 cut(s) 138
BseBI CCWGG 1 cut(s) 521
BseDI CCNNGG 2 cut(s) 150, 206
BseGI GGATG 2 cut(s) 160, 577
BseJI GATNNNNATC 1 cut(s) 138
BseLI CCNNNNNNNGG 1 cut(s) 193
BseMI GCAATG 1 cut(s) 24
BseMII CTCAG 4 cut(s) 222, 401, 407, 506
BseNI ACTGG 1 cut(s) 62
BseXI GCAGC 2 cut(s) 41, 55
BseYI CCCAGC 1 cut(s) 563
Bsh1236I CGCG 1 cut(s) 152
Bsh1285I CGRYCG 1 cut(s) 129
BshFI GGCC 3 cut(s) 177, 375, 426
BsiEI CGRYCG 1 cut(s) 129
BsiSI CCGG 1 cut(s) 206
BslI CCNNNNNNNGG 1 cut(s) 193
BsmAI GTCTC 3 cut(s) 66, 266, 396
BsnI GGCC 3 cut(s) 177, 375, 426
Bso31I GGTCTC 1 cut(s) 66
Bsp143I GATC 7 cut(s) 36, 126, 133, 139, 202, 285, 451
BspACI CCGC 5 cut(s) 96, 150, 152, 429, 659
BspANI GGCC 3 cut(s) 177, 375, 426
BspCNI CTCAG 4 cut(s) 223, 400, 406, 505
BspFNI CGCG 1 cut(s) 152
BspHI TCATGA 1 cut(s) 87
BspLI GGNNCC 1 cut(s) 427
BspPI GGATC 3 cut(s) 141, 197, 459
BspTNI GGTCTC 1 cut(s) 66
BsrDI GCAATG 1 cut(s) 24
BsrI ACTGG 1 cut(s) 62
BssECI CCNNGG 2 cut(s) 150, 206
BssMI GATC 7 cut(s) 36, 126, 133, 139, 202, 285, 451
Bst2UI CCWGG 1 cut(s) 521
Bst4CI ACNGT 2 cut(s) 304, 587
BstC8I GCNNGC 2 cut(s) 118, 373
BstDEI CTNAG 5 cut(s) 17, 231, 387, 393, 492
BstDSI CCRYGG 1 cut(s) 150
BstF5I GGATG 2 cut(s) 160, 577
BstFNI CGCG 1 cut(s) 152
BstKTI GATC 7 cut(s) 39, 129, 136, 142, 205, 288, 454
BstMAI GTCTC 3 cut(s) 66, 266, 396
BstMBI GATC 7 cut(s) 36, 126, 133, 139, 202, 285, 451
BstMCI CGRYCG 1 cut(s) 129
BstNI CCWGG 1 cut(s) 521
BstSCI CCNGG 2 cut(s) 205, 519
BstSFI CTRYAG 1 cut(s) 636
BstUI CGCG 1 cut(s) 152
BstV1I GCAGC 2 cut(s) 41, 55
BstX2I RGATCY 2 cut(s) 139, 451
BstYI RGATCY 2 cut(s) 139, 451
Bsu36I CCTNAGG 2 cut(s) 231, 492
BsuI GTATCC 1 cut(s) 587
BsuRI GGCC 3 cut(s) 177, 375, 426
BtgI CCRYGG 1 cut(s) 150
BtsCI GGATG 2 cut(s) 160, 577
BtsIMutI CAGTG 1 cut(s) 309
Cac8I GCNNGC 2 cut(s) 118, 373
CciI TCATGA 1 cut(s) 87
Cfr13I GGNCC 2 cut(s) 374, 425
Cfr42I CCGCGG 1 cut(s) 153
Csp6I GTAC 2 cut(s) 104, 461
CviAII CATG 3 cut(s) 88, 179, 536
CviJI RGCY 8 cut(s) 32, 68, 177, 211, 343, 375, 426, 641
CviKI_1 RGCY 8 cut(s) 32, 68, 177, 211, 343, 375, 426, 641
CviQI GTAC 2 cut(s) 104, 461
DdeI CTNAG 5 cut(s) 17, 231, 387, 393, 492
DpnI GATC 7 cut(s) 38, 128, 135, 141, 204, 287, 453
DpnII GATC 7 cut(s) 36, 126, 133, 139, 202, 285, 451
Eco31I GGTCTC 1 cut(s) 66
Eco57I CTGAAG 1 cut(s) 336
Eco81I CCTNAGG 2 cut(s) 231, 492
EcoRII CCWGG 1 cut(s) 519
FaeI CATG 3 cut(s) 91, 182, 539
FatI CATG 3 cut(s) 87, 178, 535
FauI CCCGC 3 cut(s) 157, 436, 666
FbaI TGATCA 1 cut(s) 36
Fnu4HI GCNGC 2 cut(s) 30, 69
FokI GGATG 2 cut(s) 167, 584
Fsp4HI GCNGC 2 cut(s) 30, 69
FspBI CTAG 1 cut(s) 332
GluI GCNGC 2 cut(s) 30, 69
GsaI CCCAGC 1 cut(s) 567
GsuI CTGGAG 1 cut(s) 48
HaeIII GGCC 3 cut(s) 177, 375, 426
HapII CCGG 1 cut(s) 206
Hin1II CATG 3 cut(s) 91, 182, 539
HincII GTYRAC 3 cut(s) 184, 438, 517
HindII GTYRAC 3 cut(s) 184, 438, 517
HinfI GANTC 2 cut(s) 385, 487
HpaI GTTAAC 1 cut(s) 517
HpaII CCGG 1 cut(s) 206
HphI GGTGA 1 cut(s) 5
Hpy166II GTNNAC 3 cut(s) 184, 438, 517
Hpy188I TCNGA 2 cut(s) 316, 613
Hpy188III TCNNGA 3 cut(s) 40, 88, 137
Hpy8I GTNNAC 3 cut(s) 184, 438, 517
Hpy99I CGWCG 1 cut(s) 425
HpyAV CCTTC 2 cut(s) 363, 562
HpyCH4III ACNGT 2 cut(s) 304, 587
HpyCH4IV ACGT 2 cut(s) 420, 434
HpyCH4V TGCA 4 cut(s) 29, 71, 116, 191
HpyF3I CTNAG 5 cut(s) 17, 231, 387, 393, 492
HpySE526I ACGT 2 cut(s) 420, 434
Hsp92II CATG 3 cut(s) 91, 182, 539
Ksp22I TGATCA 1 cut(s) 36
KspAI GTTAAC 1 cut(s) 517
KspI CCGCGG 1 cut(s) 153
Kzo9I GATC 7 cut(s) 36, 126, 133, 139, 202, 285, 451
Lsp1109I GCAGC 2 cut(s) 41, 55
MaeI CTAG 1 cut(s) 332
MaeII ACGT 2 cut(s) 420, 434
MaeIII GTNAC 1 cut(s) 625
MalI GATC 7 cut(s) 38, 128, 135, 141, 204, 287, 453
MboI GATC 7 cut(s) 36, 126, 133, 139, 202, 285, 451
MboII GAAGA 3 cut(s) 208, 427, 566
MfeI CAATTG 1 cut(s) 165
MflI RGATCY 2 cut(s) 139, 451
MluCI AATT 4 cut(s) 165, 262, 346, 542
MlyI GAGTC 1 cut(s) 379
MnlI CCTC 5 cut(s) 21, 167, 226, 501, 505
MseI TTAA 3 cut(s) 366, 516, 667
MslI CAYNNNNRTG 1 cut(s) 18
MspA1I CMGCKG 3 cut(s) 68, 98, 152
MspI CCGG 1 cut(s) 206
MspR9I CCNGG 2 cut(s) 207, 521
MunI CAATTG 1 cut(s) 165
MvaI CCWGG 1 cut(s) 521
MvnI CGCG 1 cut(s) 152
NciI CCSGG 1 cut(s) 207
NdeII GATC 7 cut(s) 36, 126, 133, 139, 202, 285, 451
NlaIII CATG 3 cut(s) 91, 182, 539
NlaIV GGNNCC 1 cut(s) 427
OliI CACNNNNGTG 1 cut(s) 18
PagI TCATGA 1 cut(s) 87
PfeI GAWTC 1 cut(s) 487
PflFI GACNNNGTC 1 cut(s) 388
PkrI GCNGC 2 cut(s) 31, 70
Ple19I CGATCG 1 cut(s) 129
PleI GAGTC 1 cut(s) 379
PpsI GAGTC 1 cut(s) 379
Psp6I CCWGG 1 cut(s) 519
PspFI CCCAGC 1 cut(s) 563
PspGI CCWGG 1 cut(s) 519
PspN4I GGNNCC 1 cut(s) 427
PspPI GGNCC 2 cut(s) 374, 425
PsuI RGATCY 2 cut(s) 139, 451
PsyI GACNNNGTC 1 cut(s) 388
PvuI CGATCG 1 cut(s) 129
PvuII CAGCTG 1 cut(s) 68
RsaI GTAC 2 cut(s) 105, 462
RsaNI GTAC 2 cut(s) 104, 461
RseI CAYNNNNRTG 1 cut(s) 18
SacII CCGCGG 1 cut(s) 153
SaqAI TTAA 3 cut(s) 366, 516, 667
SatI GCNGC 2 cut(s) 30, 69
Sau3AI GATC 7 cut(s) 36, 126, 133, 139, 202, 285, 451
Sau96I GGNCC 2 cut(s) 374, 425
SchI GAGTC 1 cut(s) 379
ScrFI CCNGG 2 cut(s) 207, 521
SfcI CTRYAG 1 cut(s) 636
Sfr303I CCGCGG 1 cut(s) 153
SgrBI CCGCGG 1 cut(s) 153
SmiMI CAYNNNNRTG 1 cut(s) 18
SmlI CTYRAG 1 cut(s) 598
SmoI CTYRAG 1 cut(s) 598
Sse9I AATT 4 cut(s) 165, 262, 346, 542
SsiI CCGC 5 cut(s) 96, 150, 152, 429, 659
SspMI CTAG 1 cut(s) 332
StyD4I CCNGG 2 cut(s) 205, 519
TaaI ACNGT 2 cut(s) 304, 587
TaiI ACGT 2 cut(s) 423, 437
TaqI TCGA 2 cut(s) 219, 540
TasI AATT 4 cut(s) 165, 262, 346, 542
TatI WGTACW 1 cut(s) 103
TfiI GAWTC 1 cut(s) 487
Tru1I TTAA 3 cut(s) 366, 516, 667
Tru9I TTAA 3 cut(s) 366, 516, 667
TscAI CASTG 1 cut(s) 309
TseI GCWGC 2 cut(s) 29, 68
TspDTI ATGAA 1 cut(s) 345
TspGWI ACGGA 1 cut(s) 421
TspRI CASTG 1 cut(s) 309
Tth111I GACNNNGTC 1 cut(s) 388
XapI RAATTY 1 cut(s) 542
XspI CTAG 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.