subtilisin-like protease
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1142 |
| Acc36I | ACCTGC | 1 cut(s) | 1142 |
| Acc65I | GGTACC | 1 cut(s) | 210 |
| AccB1I | GGYRCC | 4 cut(s) | 210, 605, 710, 754 |
| AccI | GTMKAC | 1 cut(s) | 2212 |
| AciI | CCGC | 3 cut(s) | 283, 1922, 2184 |
| AcoI | YGGCCR | 1 cut(s) | 1761 |
| AcsI | RAATTY | 1 cut(s) | 697 |
| AcuI | CTGAAG | 1 cut(s) | 1758 |
| AdeI | CACNNNGTG | 1 cut(s) | 878 |
| AfaI | GTAC | 6 cut(s) | 212, 779, 1633, 1686, 2036, 2078 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 289, 1319, 1481 |
| AflIII | ACRYGT | 1 cut(s) | 1366 |
| AjnI | CCWGG | 4 cut(s) | 1131, 1549, 1843, 2014 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1469, 1501 |
| AleI | CACNNNNGTG | 1 cut(s) | 1656 |
| Alw21I | GWGCWC | 2 cut(s) | 1159, 1952 |
| Alw26I | GTCTC | 2 cut(s) | 113, 1853 |
| Alw44I | GTGCAC | 1 cut(s) | 1155 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 746 |
| Ama87I | CYCGRG | 1 cut(s) | 254 |
| AoxI | GGCC | 4 cut(s) | 1574, 1761, 1813, 2059 |
| ApaLI | GTGCAC | 1 cut(s) | 1155 |
| ApoI | RAATTY | 1 cut(s) | 697 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1437, 1469 |
| Asp718I | GGTACC | 1 cut(s) | 210 |
| AspS9I | GGNCC | 5 cut(s) | 977, 1194, 1502, 1547, 2060 |
| AsuC2I | CCSGG | 1 cut(s) | 1691 |
| AsuHPI | GGTGA | 7 cut(s) | 473, 1399, 1472, 1643, 1692, 1921, 2216 |
| AvaI | CYCGRG | 1 cut(s) | 254 |
| AvaII | GGWCC | 4 cut(s) | 977, 1194, 1502, 1547 |
| AxyI | CCTNAGG | 1 cut(s) | 2127 |
| BaeGI | GKGCMC | 1 cut(s) | 1159 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 202, 235 |
| BanI | GGYRCC | 4 cut(s) | 210, 605, 710, 754 |
| BanII | GRGCYC | 1 cut(s) | 643 |
| Bbv12I | GWGCWC | 2 cut(s) | 1159, 1952 |
| BbvCI | CCTCAGC | 1 cut(s) | 2093 |
| BccI | CCATC | 6 cut(s) | 791, 842, 899, 1085, 1811, 2201 |
| BceAI | ACGGC | 1 cut(s) | 94 |
| BciT130I | CCWGG | 4 cut(s) | 1133, 1551, 1845, 2016 |
| BclI | TGATCA | 2 cut(s) | 187, 1462 |
| BcnI | CCSGG | 1 cut(s) | 1691 |
| BcoDI | GTCTC | 2 cut(s) | 113, 1853 |
| BfaI | CTAG | 5 cut(s) | 74, 152, 1235, 1304, 1565 |
| BfmI | CTRYAG | 7 cut(s) | 26, 422, 633, 690, 1380, 1425, 1785 |
| BfuAI | ACCTGC | 1 cut(s) | 1142 |
| BlpI | GCTNAGC | 1 cut(s) | 886 |
| Bme1390I | CCNGG | 5 cut(s) | 1133, 1551, 1691, 1845, 2016 |
| Bme18I | GGWCC | 4 cut(s) | 977, 1194, 1502, 1547 |
| BmeT110I | CYCGRG | 1 cut(s) | 254 |
| BmgT120I | GGNCC | 5 cut(s) | 977, 1194, 1502, 1547, 2060 |
| BmrFI | CCNGG | 5 cut(s) | 1133, 1551, 1691, 1845, 2016 |
| BmsI | GCATC | 1 cut(s) | 1401 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1845 |
| BpmI | CTGGAG | 3 cut(s) | 125, 528, 630 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1336, 2093 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 886 |
| BpuEI | CTTGAG | 2 cut(s) | 197, 1661 |
| BpuMI | CCSGG | 1 cut(s) | 1691 |
| BsaI | GGTCTC | 1 cut(s) | 1853 |
| BsaJI | CCNNGG | 2 cut(s) | 1010, 1843 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 843, 873, 1203, 1233 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 289, 1319, 1481 |
| Bse1I | ACTGG | 6 cut(s) | 108, 161, 301, 647, 751, 1700 |
| Bse21I | CCTNAGG | 1 cut(s) | 2127 |
| Bse3DI | GCAATG | 6 cut(s) | 292, 780, 939, 1171, 1662, 1728 |
| BseBI | CCWGG | 4 cut(s) | 1133, 1551, 1845, 2016 |
| BseDI | CCNNGG | 2 cut(s) | 1010, 1843 |
| BseGI | GGATG | 2 cut(s) | 367, 802 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 289, 1319, 1481 |
| BseMI | GCAATG | 6 cut(s) | 292, 780, 939, 1171, 1662, 1728 |
| BseMII | CTCAG | 6 cut(s) | 798, 900, 2042, 2107, 2118, 2237 |
| BseNI | ACTGG | 6 cut(s) | 108, 161, 301, 647, 751, 1700 |
| BseRI | GAGGAG | 2 cut(s) | 531, 1257 |
| BseSI | GKGCMC | 1 cut(s) | 1159 |
| BseYI | CCCAGC | 3 cut(s) | 2062, 2198, 2238 |
| BsgI | GTGCAG | 2 cut(s) | 1830, 1964 |
| BshFI | GGCC | 4 cut(s) | 1576, 1763, 1815, 2061 |
| BshNI | GGYRCC | 4 cut(s) | 210, 605, 710, 754 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1159, 1952 |
| BsiHKCI | CYCGRG | 1 cut(s) | 254 |
| BsiSI | CCGG | 2 cut(s) | 1358, 1691 |
| BslFI | GGGAC | 5 cut(s) | 390, 450, 592, 990, 1180 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 289, 1319, 1481 |
| BsmAI | GTCTC | 2 cut(s) | 113, 1853 |
| BsmFI | GGGAC | 5 cut(s) | 390, 450, 592, 990, 1180 |
| BsnI | GGCC | 4 cut(s) | 1576, 1763, 1815, 2061 |
| Bso31I | GGTCTC | 1 cut(s) | 1853 |
| BsoBI | CYCGRG | 1 cut(s) | 254 |
| Bsp1286I | GDGCHC | 3 cut(s) | 643, 1159, 1952 |
| Bsp1407I | TGTACA | 1 cut(s) | 777 |
| Bsp143I | GATC | 3 cut(s) | 30, 187, 1462 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 886 |
| Bsp19I | CCATGG | 1 cut(s) | 1010 |
| BspACI | CCGC | 3 cut(s) | 283, 1922, 2184 |
| BspANI | GGCC | 4 cut(s) | 1576, 1763, 1815, 2061 |
| BspCNI | CTCAG | 6 cut(s) | 799, 899, 2041, 2106, 2119, 2236 |
| BspMAI | CTGCAG | 5 cut(s) | 30, 426, 694, 1384, 1789 |
| BspMI | ACCTGC | 1 cut(s) | 1142 |
| BspT107I | GGYRCC | 4 cut(s) | 210, 605, 710, 754 |
| BspTNI | GGTCTC | 1 cut(s) | 1853 |
| BsrDI | GCAATG | 6 cut(s) | 292, 780, 939, 1171, 1662, 1728 |
| BsrGI | TGTACA | 1 cut(s) | 777 |
| BsrI | ACTGG | 6 cut(s) | 108, 161, 301, 647, 751, 1700 |
| BssECI | CCNNGG | 2 cut(s) | 1010, 1843 |
| BssMI | GATC | 3 cut(s) | 30, 187, 1462 |
| BssNAI | GTATAC | 1 cut(s) | 2213 |
| BssT1I | CCWWGG | 1 cut(s) | 1010 |
| Bst1107I | GTATAC | 1 cut(s) | 2213 |
| Bst2UI | CCWGG | 4 cut(s) | 1133, 1551, 1845, 2016 |
| BstAPI | GCANNNNNTGC | 5 cut(s) | 83, 761, 920, 1256, 1520 |
| BstAUI | TGTACA | 1 cut(s) | 777 |
| BstC8I | GCNNGC | 8 cut(s) | 131, 241, 715, 1033, 1470, 1516, 1756, 1813 |
| BstDSI | CCRYGG | 1 cut(s) | 1010 |
| BstEII | GGTNACC | 1 cut(s) | 1698 |
| BstF5I | GGATG | 2 cut(s) | 367, 802 |
| BstKTI | GATC | 3 cut(s) | 33, 190, 1465 |
| BstMAI | GTCTC | 2 cut(s) | 113, 1853 |
| BstMBI | GATC | 3 cut(s) | 30, 187, 1462 |
| BstNI | CCWGG | 4 cut(s) | 1133, 1551, 1845, 2016 |
| BstNSI | RCATGY | 2 cut(s) | 133, 1370 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1845 |
| BstPI | GGTNACC | 1 cut(s) | 1698 |
| BstSCI | CCNGG | 5 cut(s) | 1131, 1549, 1689, 1843, 2014 |
| BstSFI | CTRYAG | 7 cut(s) | 26, 422, 633, 690, 1380, 1425, 1785 |
| BstSLI | GKGCMC | 1 cut(s) | 1159 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 415, 552 |
| BstZ17I | GTATAC | 1 cut(s) | 2213 |
| Bsu36I | CCTNAGG | 1 cut(s) | 2127 |
| BsuRI | GGCC | 4 cut(s) | 1576, 1763, 1815, 2061 |
| BtgI | CCRYGG | 1 cut(s) | 1010 |
| BtsCI | GGATG | 2 cut(s) | 367, 802 |
| BtsI | GCAGTG | 2 cut(s) | 834, 1952 |
| BtsIMutI | CAGTG | 5 cut(s) | 324, 834, 1596, 1952, 2115 |
| BveI | ACCTGC | 1 cut(s) | 1142 |
| Cac8I | GCNNGC | 8 cut(s) | 131, 241, 715, 1033, 1470, 1516, 1756, 1813 |
| CaiI | CAGNNNCTG | 1 cut(s) | 746 |
| Cfr13I | GGNCC | 5 cut(s) | 977, 1194, 1502, 1547, 2060 |
| CseI | GACGC | 1 cut(s) | 1352 |
| CsiI | ACCWGGT | 1 cut(s) | 1131 |
| Csp6I | GTAC | 6 cut(s) | 211, 778, 1632, 1685, 2035, 2077 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 2259, 2294 |
| CviQI | GTAC | 6 cut(s) | 211, 778, 1632, 1685, 2035, 2077 |
| DpnI | GATC | 3 cut(s) | 32, 189, 1464 |
| DpnII | GATC | 3 cut(s) | 30, 187, 1462 |
| DraIII | CACNNNGTG | 1 cut(s) | 878 |
| EaeI | YGGCCR | 1 cut(s) | 1761 |
| Eco130I | CCWWGG | 1 cut(s) | 1010 |
| Eco24I | GRGCYC | 1 cut(s) | 643 |
| Eco31I | GGTCTC | 1 cut(s) | 1853 |
| Eco47I | GGWCC | 4 cut(s) | 977, 1194, 1502, 1547 |
| Eco57I | CTGAAG | 1 cut(s) | 1758 |
| Eco81I | CCTNAGG | 1 cut(s) | 2127 |
| Eco88I | CYCGRG | 1 cut(s) | 254 |
| Eco91I | GGTNACC | 1 cut(s) | 1698 |
| EcoO109I | RGGNCCY | 2 cut(s) | 977, 1194 |
| EcoO65I | GGTNACC | 1 cut(s) | 1698 |
| EcoRII | CCWGG | 4 cut(s) | 1131, 1549, 1843, 2014 |
| EcoT14I | CCWWGG | 1 cut(s) | 1010 |
| EcoT22I | ATGCAT | 1 cut(s) | 280 |
| EcoT38I | GRGCYC | 1 cut(s) | 643 |
| ErhI | CCWWGG | 1 cut(s) | 1010 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 932, 964, 1182, 1214 |
| FaqI | GGGAC | 5 cut(s) | 390, 450, 592, 990, 1180 |
| FbaI | TGATCA | 2 cut(s) | 187, 1462 |
| FblI | GTMKAC | 1 cut(s) | 2212 |
| FokI | GGATG | 2 cut(s) | 354, 809 |
| FriOI | GRGCYC | 1 cut(s) | 643 |
| FspBI | CTAG | 5 cut(s) | 74, 152, 1235, 1304, 1565 |
| GsaI | CCCAGC | 3 cut(s) | 2066, 2202, 2242 |
| GsuI | CTGGAG | 3 cut(s) | 125, 528, 630 |
| HaeIII | GGCC | 4 cut(s) | 1576, 1763, 1815, 2061 |
| HapII | CCGG | 2 cut(s) | 1358, 1691 |
| HgaI | GACGC | 1 cut(s) | 1352 |
| HincII | GTYRAC | 3 cut(s) | 1309, 1822, 2152 |
| HindII | GTYRAC | 3 cut(s) | 1309, 1822, 2152 |
| HindIII | AAGCTT | 1 cut(s) | 1067 |
| HinfI | GANTC | 8 cut(s) | 488, 809, 945, 1015, 1097, 1169, 1300, 1601 |
| HpaI | GTTAAC | 2 cut(s) | 1822, 2152 |
| HpaII | CCGG | 2 cut(s) | 1358, 1691 |
| HphI | GGTGA | 7 cut(s) | 473, 1399, 1472, 1643, 1692, 1921, 2216 |
| Hpy188I | TCNGA | 8 cut(s) | 187, 793, 808, 814, 990, 1129, 2031, 2320 |
| Hpy188III | TCNNGA | 5 cut(s) | 256, 371, 1497, 1678, 1769 |
| Hpy99I | CGWCG | 1 cut(s) | 527 |
| HpyCH4IV | ACGT | 1 cut(s) | 2075 |
| HpySE526I | ACGT | 1 cut(s) | 2075 |
| KflI | GGGWCCC | 1 cut(s) | 1194 |
| KpnI | GGTACC | 1 cut(s) | 214 |
| Ksp22I | TGATCA | 2 cut(s) | 187, 1462 |
| KspAI | GTTAAC | 2 cut(s) | 1822, 2152 |
| Kzo9I | GATC | 3 cut(s) | 30, 187, 1462 |
| LmnI | GCTCC | 8 cut(s) | 106, 638, 929, 1012, 1028, 1477, 1961, 2092 |
| LweI | GCATC | 1 cut(s) | 1401 |
| MabI | ACCWGGT | 1 cut(s) | 1131 |
| MaeI | CTAG | 5 cut(s) | 74, 152, 1235, 1304, 1565 |
| MaeII | ACGT | 1 cut(s) | 2075 |
| MaeIII | GTNAC | 3 cut(s) | 69, 566, 1698 |
| MalI | GATC | 3 cut(s) | 32, 189, 1464 |
| MboI | GATC | 3 cut(s) | 30, 187, 1462 |
| MboII | GAAGA | 2 cut(s) | 355, 1480 |
| MfeI | CAATTG | 1 cut(s) | 78 |
| MhlI | GDGCHC | 3 cut(s) | 643, 1159, 1952 |
| MluCI | AATT | 9 cut(s) | 78, 454, 697, 909, 1254, 1900, 2068, 2081, 2159 |
| MlyI | GAGTC | 2 cut(s) | 818, 1595 |
| MmeI | TCCRAC | 3 cut(s) | 145, 1006, 1189 |
| Mph1103I | ATGCAT | 1 cut(s) | 280 |
| MseI | TTAA | 3 cut(s) | 1713, 1821, 2151 |
| MslI | CAYNNNNRTG | 4 cut(s) | 765, 1164, 1656, 2117 |
| MspA1I | CMGCKG | 5 cut(s) | 689, 957, 965, 1706, 1736 |
| MspI | CCGG | 2 cut(s) | 1358, 1691 |
| MspR9I | CCNGG | 5 cut(s) | 1133, 1551, 1691, 1845, 2016 |
| MunI | CAATTG | 1 cut(s) | 78 |
| MvaI | CCWGG | 4 cut(s) | 1133, 1551, 1845, 2016 |
| NciI | CCSGG | 1 cut(s) | 1691 |
| NcoI | CCATGG | 1 cut(s) | 1010 |
| NdeII | GATC | 3 cut(s) | 30, 187, 1462 |
| NmeAIII | GCCGAG | 2 cut(s) | 142, 1739 |
| NmuCI | GTSAC | 3 cut(s) | 69, 566, 1698 |
| NsiI | ATGCAT | 1 cut(s) | 280 |
| NspI | RCATGY | 2 cut(s) | 133, 1370 |
| OliI | CACNNNNGTG | 1 cut(s) | 1656 |
| PaeI | GCATGC | 1 cut(s) | 133 |
| PaeR7I | CTCGAG | 1 cut(s) | 254 |
| PaqCI | CACCTGC | 1 cut(s) | 1142 |
| PciI | ACATGT | 1 cut(s) | 1366 |
| PfeI | GAWTC | 6 cut(s) | 488, 945, 1015, 1097, 1169, 1300 |
| PleI | GAGTC | 2 cut(s) | 817, 1595 |
| PpsI | GAGTC | 2 cut(s) | 817, 1595 |
| PpuMI | RGGWCCY | 2 cut(s) | 977, 1194 |
| PscI | ACATGT | 1 cut(s) | 1366 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1845 |
| Psp5II | RGGWCCY | 2 cut(s) | 977, 1194 |
| Psp6I | CCWGG | 4 cut(s) | 1131, 1549, 1843, 2014 |
| PspEI | GGTNACC | 1 cut(s) | 1698 |
| PspFI | CCCAGC | 3 cut(s) | 2062, 2198, 2238 |
| PspGI | CCWGG | 4 cut(s) | 1131, 1549, 1843, 2014 |
| PspPI | GGNCC | 5 cut(s) | 977, 1194, 1502, 1547, 2060 |
| PspPPI | RGGWCCY | 2 cut(s) | 977, 1194 |
| PstI | CTGCAG | 5 cut(s) | 30, 426, 694, 1384, 1789 |
| PstNI | CAGNNNCTG | 1 cut(s) | 746 |
| PvuII | CAGCTG | 5 cut(s) | 689, 957, 965, 1706, 1736 |
| RsaI | GTAC | 6 cut(s) | 212, 779, 1633, 1686, 2036, 2078 |
| RsaNI | GTAC | 6 cut(s) | 211, 778, 1632, 1685, 2035, 2077 |
| RseI | CAYNNNNRTG | 4 cut(s) | 765, 1164, 1656, 2117 |
| SaqAI | TTAA | 3 cut(s) | 1713, 1821, 2151 |
| Sau3AI | GATC | 3 cut(s) | 30, 187, 1462 |
| Sau96I | GGNCC | 5 cut(s) | 977, 1194, 1502, 1547, 2060 |
| SchI | GAGTC | 2 cut(s) | 818, 1595 |
| ScrFI | CCNGG | 5 cut(s) | 1133, 1551, 1691, 1845, 2016 |
| SduI | GDGCHC | 3 cut(s) | 643, 1159, 1952 |
| SexAI | ACCWGGT | 1 cut(s) | 1131 |
| SfaNI | GCATC | 1 cut(s) | 1401 |
| SfcI | CTRYAG | 7 cut(s) | 26, 422, 633, 690, 1380, 1425, 1785 |
| Sfr274I | CTCGAG | 1 cut(s) | 254 |
| SinI | GGWCC | 4 cut(s) | 977, 1194, 1502, 1547 |
| SlaI | CTCGAG | 1 cut(s) | 254 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 765, 1164, 1656, 2117 |
| SmlI | CTYRAG | 3 cut(s) | 176, 254, 1676 |
| SmoI | CTYRAG | 3 cut(s) | 176, 254, 1676 |
| SphI | GCATGC | 1 cut(s) | 133 |
| Sse9I | AATT | 9 cut(s) | 78, 454, 697, 909, 1254, 1900, 2068, 2081, 2159 |
| SsiI | CCGC | 3 cut(s) | 283, 1922, 2184 |
| SspI | AATATT | 1 cut(s) | 1527 |
| SspMI | CTAG | 5 cut(s) | 74, 152, 1235, 1304, 1565 |
| StyD4I | CCNGG | 5 cut(s) | 1131, 1549, 1689, 1843, 2014 |
| StyI | CCWWGG | 1 cut(s) | 1010 |
| TaiI | ACGT | 1 cut(s) | 2078 |
| TaqI | TCGA | 1 cut(s) | 255 |
| TasI | AATT | 9 cut(s) | 78, 454, 697, 909, 1254, 1900, 2068, 2081, 2159 |
| TatI | WGTACW | 2 cut(s) | 777, 2034 |
| TauI | GCSGC | 2 cut(s) | 1925, 2186 |
| TfiI | GAWTC | 6 cut(s) | 488, 945, 1015, 1097, 1169, 1300 |
| Tru1I | TTAA | 3 cut(s) | 1713, 1821, 2151 |
| Tru9I | TTAA | 3 cut(s) | 1713, 1821, 2151 |
| TscAI | CASTG | 5 cut(s) | 324, 841, 1603, 1952, 2122 |
| TseFI | GTSAC | 3 cut(s) | 69, 566, 1698 |
| Tsp45I | GTSAC | 3 cut(s) | 69, 566, 1698 |
| TspDTI | ATGAA | 8 cut(s) | 46, 504, 672, 1017, 1182, 1203, 1788, 2123 |
| TspRI | CASTG | 5 cut(s) | 324, 841, 1603, 1952, 2122 |
| VneI | GTGCAC | 1 cut(s) | 1155 |
| VpaK11BI | GGWCC | 4 cut(s) | 977, 1194, 1502, 1547 |
| XapI | RAATTY | 1 cut(s) | 697 |
| XceI | RCATGY | 2 cut(s) | 133, 1370 |
| XhoI | CTCGAG | 1 cut(s) | 254 |
| XmiI | GTMKAC | 1 cut(s) | 2212 |
| XspI | CTAG | 5 cut(s) | 74, 152, 1235, 1304, 1565 |
| Zsp2I | ATGCAT | 1 cut(s) | 280 |