Rroxscaffold_3G00256240

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000302
Physical Location & Seq
Forward (+)
50627338 .. 50628858
1521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00256240.1

Sequence Viewer

Length: 1521 bp
ATGTACAAAGTCTGCTCTGAACAGGGTTGTTTTGATTCGGACATTCTAGCTGCTATGGACACTGCGGTTGATGATGGTGTGGATGTGCTATCGCTCTCACTTGGAGGTGGCTCAGCTTATTTTTACGCTGATGGAATTGCAGTCGGTGCATTTGGAGAAATTCAGAAGGGAATTTTTGTCAGCTGTTCGGCTGGAAATGAGGGACCTGACTACAATACTTTATCAAATGAGGCCCCATGGATTCTCACAGTTGGAGCAAGCACCATTGACAGGAGCATAAAAGCAACAGCAAAGCTTGGAAATGGGCAAGAATATGACGGGGAGTCAGTATTCCTGCCTAAAGATTTTAGTTCAAAACAGTTACCTCTTGTTTATGCAGGTGTATATAGCGATGATTCATCAGCTCTTTGCGATGTAGGGTCCCTTACAAATGTTGAAGGGAAAGTAGTGGTGTGTGAGGTAGGTGGAGCAATTGGAAGAATTGCAAAAGGGGTAGAAGTGGAAAGAGCTGGTGGTGTTGCCATGATTCTTGTCAACCAAGACTTCGCTGGCTATACCACCATAGCAGACGCTCATGTGCTTCCGGCAACACATGTGAGTTACTCTGCAGGGGTGAGCATCAAAACCTATATAAACTCAACCTCAACTCCTACAGCAACAATCTTGTTCAAAGGCACTGTCATTGGTGATCAGCTAGCTCCGAAGGTCTCTTTCTTTTCATCGAGAGGACCAAGCCTCGCAAGTCCTGGCATTTTGAAACCTGACATTATTGGTCCCGGTGTGAGCATCCTAGCTGCATGGCCTTTTTCGGTGGATAATGCCACAGAATCTAAAGCCACATTTAACATTATTTCTGGTACTTCAATGTCAAGCCCTCACCTAAGTGGCATTGTAGCCTTGCTCAAGAGCTCACACCCTGACTGGTCACCAGCTGCCATTAAGTCTGCCATCATGACAACTGCTGAAGTAAACAACCTCGCAGGCTCCGCCTTAATGGATGAAACACTTTTTGCAGCAGACCTCTTTGCCATTGGTGCAGGTCATGTTAACCCTTCAAAAGCAAATGACCCTGGGCTCATCTACGACACGCAACCAGAGGAGTACATTCCTTACTTGTGTGGTTTAAACTACACAGACAAACAGATAGCGGTAATCACCCAAAAATCAGTGAAATGCTCTGAAGTAGGAGCCATACCAGAAGCACAGCTAAATTATCCCACATTTTCTCTCTTTATAGATCCTGCTGGGAAGCCTCAGAAGTACACAAGAACAGTGACGAATGTTGGCCCCGCTAAATCAACTTATGAATTAGCTGTTGTAAGTCCACATAAGATAGACATCAGTGTGCAACCTGAGGAGCTCACATTCACAGAGATTAACCAGACAATGACATACCATGTGGTGTTTACTGCACAAAAAGGTGCTGCGAAGGATGGTATAACATTTTCTCAGGGATATCTGAGCTGGGTCTCTCGCCAGCATTCTGTTAGAAGCCAAATATCGGTGATCTTTGACACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

506

Amino Acids

53.28

Weight (kDa)

4.82

Isoelectric Point (pI)

29.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 2 - 324 3.1e-30 Subtilase family
PA PF02225 120 - 206 4.2e-11 PA domain
fn3_6 PF17766 402 - 502 2.1e-26 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 368
Acc36I ACCTGC 2 cut(s) 368, 1028
AciI CCGC 4 cut(s) 65, 987, 1148, 1290
AclWI GGATC 1 cut(s) 1232
AcsI RAATTY 2 cut(s) 159, 171
AcuI CTGAAG 2 cut(s) 984, 1200
AfaI GTAC 4 cut(s) 5, 859, 1103, 1262
AfiI CCNNNNNNNGG 2 cut(s) 270, 1501
AflIII ACRYGT 1 cut(s) 592
AgsI TTSAA 6 cut(s) 354, 437, 670, 757, 864, 1056
AhdI GACNNNNNGTC 1 cut(s) 322
AjnI CCWGG 2 cut(s) 745, 1069
AleI CACNNNNGTG 1 cut(s) 882
Alw21I GWGCWC 2 cut(s) 911, 1362
Alw26I GTCTC 2 cut(s) 712, 1474
AlwI GGATC 1 cut(s) 1232
AoxI GGCC 3 cut(s) 231, 800, 1285
ApeKI GCWGC 5 cut(s) 50, 794, 932, 1013, 1424
ApoI RAATTY 2 cut(s) 159, 171
ArsI GACNNNNNNTTYG 2 cut(s) 663, 695
AspS9I GGNCC 6 cut(s) 203, 232, 420, 728, 773, 1286
AsuC2I CCSGG 1 cut(s) 777
AsuHPI GGTGA 6 cut(s) 625, 698, 869, 918, 1147, 1516
AsuNHI GCTAGC 1 cut(s) 694
AvaII GGWCC 4 cut(s) 203, 420, 728, 773
AxyI CCTNAGG 1 cut(s) 1353
BanII GRGCYC 3 cut(s) 911, 1077, 1362
Bbv12I GWGCWC 2 cut(s) 911, 1362
BbvI GCAGC 5 cut(s) 37, 781, 919, 1025, 1411
BccI CCATC 4 cut(s) 68, 125, 956, 1427
BciT130I CCWGG 2 cut(s) 747, 1071
BclI TGATCA 1 cut(s) 688
BcnI CCSGG 1 cut(s) 777
BcoDI GTCTC 2 cut(s) 712, 1474
BfaI CTAG 3 cut(s) 47, 695, 791
BfmI CTRYAG 2 cut(s) 606, 651
BfuAI ACCTGC 2 cut(s) 368, 1028
BisI GCNGC 5 cut(s) 51, 795, 933, 1014, 1425
BlpI GCTNAGC 1 cut(s) 112
BlsI GCNGC 5 cut(s) 52, 796, 934, 1015, 1426
Bme1390I CCNGG 3 cut(s) 747, 777, 1071
Bme18I GGWCC 4 cut(s) 203, 420, 728, 773
BmeRI GACNNNNNGTC 1 cut(s) 322
BmgT120I GGNCC 6 cut(s) 203, 232, 420, 728, 773, 1286
BmiI GGNNCC 8 cut(s) 204, 234, 421, 422, 775, 985, 1189, 1288
BmrFI CCNGG 3 cut(s) 747, 777, 1071
BmsI GCATC 2 cut(s) 627, 795
BmtI GCTAGC 1 cut(s) 698
Bpu1102I GCTNAGC 1 cut(s) 112
BpuEI CTTGAG 1 cut(s) 887
BpuMI CCSGG 1 cut(s) 777
BsaBI GATNNNNATC 1 cut(s) 1337
BsaI GGTCTC 2 cut(s) 712, 1474
BsaJI CCNNGG 3 cut(s) 236, 1069, 1070
BsaXI ACNNNNNCTCC 2 cut(s) 631, 661
Bsc4I CCNNNNNNNGG 2 cut(s) 270, 1501
Bse1I ACTGG 1 cut(s) 926
Bse21I CCTNAGG 1 cut(s) 1353
Bse8I GATNNNNATC 1 cut(s) 1337
BseBI CCWGG 2 cut(s) 747, 1071
BseDI CCNNGG 3 cut(s) 236, 1069, 1070
BseGI GGATG 4 cut(s) 88, 786, 1003, 1438
BseJI GATNNNNATC 1 cut(s) 1337
BseLI CCNNNNNNNGG 2 cut(s) 270, 1501
BseMII CTCAG 5 cut(s) 126, 1268, 1344, 1451, 1463
BseNI ACTGG 1 cut(s) 926
BseRI GAGGAG 2 cut(s) 1112, 1370
BseXI GCAGC 5 cut(s) 37, 781, 919, 1025, 1411
BseYI CCCAGC 2 cut(s) 1244, 1464
BsgI GTGCAG 2 cut(s) 1056, 1395
BshFI GGCC 3 cut(s) 233, 802, 1287
BsiHKAI GWGCWC 2 cut(s) 911, 1362
BsiSI CCGG 2 cut(s) 584, 777
BslFI GGGAC 3 cut(s) 216, 406, 759
BslI CCNNNNNNNGG 2 cut(s) 270, 1501
BsmAI GTCTC 2 cut(s) 712, 1474
BsmFI GGGAC 3 cut(s) 216, 406, 759
BsmI GAATGC 1 cut(s) 1480
BsnI GGCC 3 cut(s) 233, 802, 1287
Bso31I GGTCTC 2 cut(s) 712, 1474
Bsp1286I GDGCHC 3 cut(s) 911, 1077, 1362
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 3 cut(s) 688, 1237, 1506
Bsp1720I GCTNAGC 1 cut(s) 112
Bsp19I CCATGG 1 cut(s) 236
BspACI CCGC 4 cut(s) 65, 987, 1148, 1290
BspANI GGCC 3 cut(s) 233, 802, 1287
BspCNI CTCAG 5 cut(s) 125, 1267, 1345, 1452, 1462
BspHI TCATGA 1 cut(s) 951
BspLI GGNNCC 8 cut(s) 204, 234, 421, 422, 775, 985, 1189, 1288
BspMAI CTGCAG 1 cut(s) 610
BspMI ACCTGC 2 cut(s) 368, 1028
BspOI GCTAGC 1 cut(s) 698
BspPI GGATC 1 cut(s) 1232
BspTNI GGTCTC 2 cut(s) 712, 1474
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 1 cut(s) 926
BssECI CCNNGG 3 cut(s) 236, 1069, 1070
BssMI GATC 3 cut(s) 688, 1237, 1506
BssT1I CCWWGG 1 cut(s) 236
Bst2UI CCWGG 2 cut(s) 747, 1071
Bst4CI ACNGT 4 cut(s) 250, 360, 679, 1273
BstAPI GCANNNNNTGC 1 cut(s) 146
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 5 cut(s) 259, 550, 696, 982, 1478
BstDEI CTNAG 7 cut(s) 112, 881, 1254, 1353, 1449, 1460, 1518
BstDSI CCRYGG 1 cut(s) 236
BstEII GGTNACC 1 cut(s) 924
BstF5I GGATG 4 cut(s) 88, 786, 1003, 1438
BstKTI GATC 3 cut(s) 691, 1240, 1509
BstMAI GTCTC 2 cut(s) 712, 1474
BstMBI GATC 3 cut(s) 688, 1237, 1506
BstMWI GCNNNNNNNGC 3 cut(s) 146, 986, 1034
BstNI CCWGG 2 cut(s) 747, 1071
BstNSI RCATGY 1 cut(s) 596
BstPI GGTNACC 1 cut(s) 924
BstSCI CCNGG 3 cut(s) 745, 775, 1069
BstSFI CTRYAG 2 cut(s) 606, 651
BstV1I GCAGC 5 cut(s) 37, 781, 919, 1025, 1411
BstX2I RGATCY 1 cut(s) 1237
BstYI RGATCY 1 cut(s) 1237
Bsu36I CCTNAGG 1 cut(s) 1353
BsuRI GGCC 3 cut(s) 233, 802, 1287
BtgI CCRYGG 1 cut(s) 236
BtgZI GCGATG 2 cut(s) 405, 426
BtsCI GGATG 4 cut(s) 88, 786, 1003, 1438
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 5 cut(s) 60, 675, 1173, 1278, 1348
BveI ACCTGC 2 cut(s) 368, 1028
Cac8I GCNNGC 5 cut(s) 259, 550, 696, 982, 1478
CciI TCATGA 1 cut(s) 951
Cfr13I GGNCC 6 cut(s) 203, 232, 420, 728, 773, 1286
CseI GACGC 1 cut(s) 578
Csp6I GTAC 4 cut(s) 4, 858, 1102, 1261
CviAII CATG 8 cut(s) 237, 523, 575, 593, 798, 952, 1043, 1397
CviQI GTAC 4 cut(s) 4, 858, 1102, 1261
DdeI CTNAG 7 cut(s) 112, 881, 1254, 1353, 1449, 1460, 1518
DpnI GATC 3 cut(s) 690, 1239, 1508
DpnII GATC 3 cut(s) 688, 1237, 1506
DraI TTTAAA 1 cut(s) 1125
DriI GACNNNNNGTC 1 cut(s) 322
Eam1105I GACNNNNNGTC 1 cut(s) 322
EciI GGCGGA 1 cut(s) 976
Ecl136II GAGCTC 2 cut(s) 909, 1360
Eco130I CCWWGG 1 cut(s) 236
Eco24I GRGCYC 3 cut(s) 911, 1077, 1362
Eco31I GGTCTC 2 cut(s) 712, 1474
Eco32I GATATC 1 cut(s) 1457
Eco47I GGWCC 4 cut(s) 203, 420, 728, 773
Eco53kI GAGCTC 2 cut(s) 909, 1360
Eco57I CTGAAG 2 cut(s) 984, 1200
Eco81I CCTNAGG 1 cut(s) 1353
Eco91I GGTNACC 1 cut(s) 924
EcoICRI GAGCTC 2 cut(s) 909, 1360
EcoO109I RGGNCCY 3 cut(s) 203, 232, 420
EcoO65I GGTNACC 1 cut(s) 924
EcoRII CCWGG 2 cut(s) 745, 1069
EcoRV GATATC 1 cut(s) 1457
EcoT14I CCWWGG 1 cut(s) 236
EcoT38I GRGCYC 3 cut(s) 911, 1077, 1362
ErhI CCWWGG 1 cut(s) 236
FaeI CATG 8 cut(s) 240, 526, 578, 596, 801, 955, 1046, 1400
FaqI GGGAC 3 cut(s) 216, 406, 759
FatI CATG 8 cut(s) 236, 522, 574, 592, 797, 951, 1042, 1396
FauI CCCGC 1 cut(s) 1297
FbaI TGATCA 1 cut(s) 688
Fnu4HI GCNGC 5 cut(s) 51, 795, 933, 1014, 1425
FokI GGATG 4 cut(s) 95, 773, 1010, 1445
FriOI GRGCYC 3 cut(s) 911, 1077, 1362
Fsp4HI GCNGC 5 cut(s) 51, 795, 933, 1014, 1425
FspBI CTAG 3 cut(s) 47, 695, 791
GluI GCNGC 5 cut(s) 51, 795, 933, 1014, 1425
GsaI CCCAGC 2 cut(s) 1248, 1468
HaeIII GGCC 3 cut(s) 233, 802, 1287
HapII CCGG 2 cut(s) 584, 777
HgaI GACGC 1 cut(s) 578
Hin1II CATG 8 cut(s) 240, 526, 578, 596, 801, 955, 1046, 1400
HincII GTYRAC 2 cut(s) 535, 1048
HindII GTYRAC 2 cut(s) 535, 1048
HindIII AAGCTT 1 cut(s) 293
HinfI GANTC 6 cut(s) 35, 241, 323, 395, 526, 827
HpaI GTTAAC 1 cut(s) 1048
HpaII CCGG 2 cut(s) 584, 777
HphI GGTGA 6 cut(s) 625, 698, 869, 918, 1147, 1516
Hpy166II GTNNAC 6 cut(s) 535, 970, 1048, 1263, 1325, 1407
Hpy188I TCNGA 7 cut(s) 19, 40, 165, 702, 1180, 1257, 1461
Hpy188III TCNNGA 3 cut(s) 723, 904, 952
Hpy8I GTNNAC 6 cut(s) 535, 970, 1048, 1263, 1325, 1407
HpyAV CCTTC 5 cut(s) 160, 431, 697, 1062, 1423
HpyCH4III ACNGT 4 cut(s) 250, 360, 679, 1273
HpyF10VI GCNNNNNNNGC 3 cut(s) 146, 986, 1034
HpyF3I CTNAG 7 cut(s) 112, 881, 1254, 1353, 1449, 1460, 1518
Hsp92II CATG 8 cut(s) 240, 526, 578, 596, 801, 955, 1046, 1400
KflI GGGWCCC 1 cut(s) 420
Ksp22I TGATCA 1 cut(s) 688
KspAI GTTAAC 1 cut(s) 1048
Kzo9I GATC 3 cut(s) 688, 1237, 1506
LmnI GCTCC 7 cut(s) 254, 273, 467, 703, 989, 1187, 1357
Lsp1109I GCAGC 5 cut(s) 37, 781, 919, 1025, 1411
LweI GCATC 2 cut(s) 627, 795
MaeI CTAG 3 cut(s) 47, 695, 791
MaeIII GTNAC 4 cut(s) 360, 599, 924, 1273
MalI GATC 3 cut(s) 690, 1239, 1508
MboI GATC 3 cut(s) 688, 1237, 1506
MboII GAAGA 1 cut(s) 489
MfeI CAATTG 1 cut(s) 471
MflI RGATCY 1 cut(s) 1237
MhlI GDGCHC 3 cut(s) 911, 1077, 1362
MluCI AATT 7 cut(s) 135, 159, 171, 471, 480, 1210, 1307
MlyI GAGTC 1 cut(s) 332
MmeI TCCRAC 1 cut(s) 232
MseI TTAA 6 cut(s) 843, 939, 992, 1047, 1124, 1377
MslI CAYNNNNRTG 2 cut(s) 882, 1343
MspA1I CMGCKG 2 cut(s) 183, 932
MspI CCGG 2 cut(s) 584, 777
MspR9I CCNGG 3 cut(s) 747, 777, 1071
MssI GTTTAAAC 1 cut(s) 1125
MunI CAATTG 1 cut(s) 471
Mva1269I GAATGC 1 cut(s) 1480
MvaI CCWGG 2 cut(s) 747, 1071
MwoI GCNNNNNNNGC 3 cut(s) 146, 986, 1034
NciI CCSGG 1 cut(s) 777
NcoI CCATGG 1 cut(s) 236
NdeII GATC 3 cut(s) 688, 1237, 1506
NheI GCTAGC 1 cut(s) 694
NlaIII CATG 8 cut(s) 240, 526, 578, 596, 801, 955, 1046, 1400
NlaIV GGNNCC 8 cut(s) 204, 234, 421, 422, 775, 985, 1189, 1288
NmuCI GTSAC 2 cut(s) 924, 1273
NspI RCATGY 1 cut(s) 596
OliI CACNNNNGTG 1 cut(s) 882
PagI TCATGA 1 cut(s) 951
PaqCI CACCTGC 1 cut(s) 368
PasI CCCWGGG 1 cut(s) 1070
PciI ACATGT 1 cut(s) 592
PctI GAATGC 1 cut(s) 1480
PfeI GAWTC 5 cut(s) 35, 241, 395, 526, 827
PkrI GCNGC 5 cut(s) 52, 796, 934, 1015, 1426
PleI GAGTC 1 cut(s) 331
PmeI GTTTAAAC 1 cut(s) 1125
PpsI GAGTC 1 cut(s) 331
PpuMI RGGWCCY 2 cut(s) 203, 420
PscI ACATGT 1 cut(s) 592
Psp124BI GAGCTC 2 cut(s) 911, 1362
Psp5II RGGWCCY 2 cut(s) 203, 420
Psp6I CCWGG 2 cut(s) 745, 1069
PspEI GGTNACC 1 cut(s) 924
PspFI CCCAGC 2 cut(s) 1244, 1464
PspGI CCWGG 2 cut(s) 745, 1069
PspN4I GGNNCC 8 cut(s) 204, 234, 421, 422, 775, 985, 1189, 1288
PspPI GGNCC 6 cut(s) 203, 232, 420, 728, 773, 1286
PspPPI RGGWCCY 2 cut(s) 203, 420
PstI CTGCAG 1 cut(s) 610
PsuI RGATCY 1 cut(s) 1237
PvuII CAGCTG 2 cut(s) 183, 932
RsaI GTAC 4 cut(s) 5, 859, 1103, 1262
RsaNI GTAC 4 cut(s) 4, 858, 1102, 1261
RseI CAYNNNNRTG 2 cut(s) 882, 1343
SacI GAGCTC 2 cut(s) 911, 1362
SaqAI TTAA 6 cut(s) 843, 939, 992, 1047, 1124, 1377
SatI GCNGC 5 cut(s) 51, 795, 933, 1014, 1425
Sau3AI GATC 3 cut(s) 688, 1237, 1506
Sau96I GGNCC 6 cut(s) 203, 232, 420, 728, 773, 1286
SchI GAGTC 1 cut(s) 332
ScrFI CCNGG 3 cut(s) 747, 777, 1071
SduI GDGCHC 3 cut(s) 911, 1077, 1362
SfaNI GCATC 2 cut(s) 627, 795
SfcI CTRYAG 2 cut(s) 606, 651
SinI GGWCC 4 cut(s) 203, 420, 728, 773
SmiMI CAYNNNNRTG 2 cut(s) 882, 1343
SmlI CTYRAG 1 cut(s) 902
SmoI CTYRAG 1 cut(s) 902
Sse9I AATT 7 cut(s) 135, 159, 171, 471, 480, 1210, 1307
SsiI CCGC 4 cut(s) 65, 987, 1148, 1290
SspMI CTAG 3 cut(s) 47, 695, 791
SstI GAGCTC 2 cut(s) 911, 1362
StyD4I CCNGG 3 cut(s) 745, 775, 1069
StyI CCWWGG 1 cut(s) 236
TaaI ACNGT 4 cut(s) 250, 360, 679, 1273
TaqI TCGA 1 cut(s) 722
TasI AATT 7 cut(s) 135, 159, 171, 471, 480, 1210, 1307
TatI WGTACW 3 cut(s) 3, 1101, 1260
TfiI GAWTC 5 cut(s) 35, 241, 395, 526, 827
Tru1I TTAA 6 cut(s) 843, 939, 992, 1047, 1124, 1377
Tru9I TTAA 6 cut(s) 843, 939, 992, 1047, 1124, 1377
TscAI CASTG 5 cut(s) 67, 682, 1173, 1278, 1348
TseFI GTSAC 2 cut(s) 924, 1273
TseI GCWGC 5 cut(s) 50, 794, 932, 1013, 1424
Tsp45I GTSAC 2 cut(s) 924, 1273
TspDTI ATGAA 4 cut(s) 387, 708, 1014, 1320
TspRI CASTG 5 cut(s) 67, 682, 1173, 1278, 1348
VpaK11BI GGWCC 4 cut(s) 203, 420, 728, 773
XapI RAATTY 2 cut(s) 159, 171
XceI RCATGY 1 cut(s) 596
XcmI CCANNNNNNNNNTGG 1 cut(s) 545
XspI CTAG 3 cut(s) 47, 695, 791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.