RLG00000028999

Subtilisin-like serine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000302
Physical Location & Seq
Forward (+)
31229570 .. 31229944
375 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028999

Sequence Viewer

Length: 375 bp
ATGATCGCCCAACAAAGAGTGGACTGCTCTGAGGTAGGAGTCACAGCTGAAGCGCAGCTAAACTATCCTTCATTTTCTATCACAATAGGGTCCTATCAAACTCAGAATTATACAAGAACTGTGACAAATGTTGGCCCGGCTTATTCAACCTACAAATCGGAGATTTTGAAACCCGAGAAATGGGGTGTGAGTGTGAACATGATCGTGACTCCTTCGGTGCTTACATTCACAGAGGTCAACCAAACCATTACAAACAATGTGGAGTTCTCCACACAACTAGCTGGGCAAGATGGTTCATTTTCACAGGGATATTTGAGATGGGTTTCTGACAAGTATTCTGTATACAGCCCGATAGCTGTCATCTTTCACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

125

Amino Acids

13.78

Weight (kDa)

5.19

Isoelectric Point (pI)

25.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_6 PF17766 19 - 120 2.7e-23 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 342
AcuI CTGAAG 1 cut(s) 69
AfiI CCNNNNNNNGG 1 cut(s) 180
AgsI TTSAA 2 cut(s) 147, 169
AluBI AGCT 4 cut(s) 47, 58, 281, 356
AluI AGCT 4 cut(s) 47, 58, 281, 356
Ama87I CYCGRG 1 cut(s) 173
AoxI GGCC 1 cut(s) 133
ApeKI GCWGC 1 cut(s) 55
AspLEI GCGC 1 cut(s) 55
AspS9I GGNCC 2 cut(s) 90, 134
AsuC2I CCSGG 1 cut(s) 137
AvaI CYCGRG 1 cut(s) 173
AvaII GGWCC 1 cut(s) 90
BbvI GCAGC 1 cut(s) 67
BccI CCATC 2 cut(s) 284, 312
BcnI CCSGG 1 cut(s) 137
BfaI CTAG 1 cut(s) 278
BisI GCNGC 1 cut(s) 56
BlsI GCNGC 1 cut(s) 57
Bme1390I CCNGG 1 cut(s) 137
Bme18I GGWCC 1 cut(s) 90
BmeT110I CYCGRG 1 cut(s) 173
BmgT120I GGNCC 2 cut(s) 90, 134
BmiI GGNNCC 1 cut(s) 91
BmrFI CCNGG 1 cut(s) 137
BpuMI CCSGG 1 cut(s) 137
Bsc4I CCNNNNNNNGG 1 cut(s) 180
BseLI CCNNNNNNNGG 1 cut(s) 180
BseMII CTCAG 2 cut(s) 21, 116
BseXI GCAGC 1 cut(s) 67
BseYI CCCAGC 1 cut(s) 281
BshFI GGCC 1 cut(s) 135
BsiHKCI CYCGRG 1 cut(s) 173
BsiSI CCGG 1 cut(s) 137
BslI CCNNNNNNNGG 1 cut(s) 180
BsnI GGCC 1 cut(s) 135
BsoBI CYCGRG 1 cut(s) 173
Bsp143I GATC 2 cut(s) 3, 201
BspANI GGCC 1 cut(s) 135
BspCNI CTCAG 2 cut(s) 22, 115
BspLI GGNNCC 1 cut(s) 91
BssMI GATC 2 cut(s) 3, 201
BssNAI GTATAC 1 cut(s) 343
Bst1107I GTATAC 1 cut(s) 343
Bst4CI ACNGT 1 cut(s) 121
BstDEI CTNAG 2 cut(s) 30, 102
BstHHI GCGC 1 cut(s) 55
BstKTI GATC 2 cut(s) 6, 204
BstMBI GATC 2 cut(s) 3, 201
BstSCI CCNGG 1 cut(s) 135
BstV1I GCAGC 1 cut(s) 67
BstZ17I GTATAC 1 cut(s) 343
BsuRI GGCC 1 cut(s) 135
CfoI GCGC 1 cut(s) 55
Cfr13I GGNCC 2 cut(s) 90, 134
CspCI CAANNNNNGTGG 2 cut(s) 240, 275
CviAII CATG 1 cut(s) 199
CviJI RGCY 7 cut(s) 47, 58, 135, 140, 281, 348, 356
CviKI_1 RGCY 7 cut(s) 47, 58, 135, 140, 281, 348, 356
DdeI CTNAG 2 cut(s) 30, 102
DpnI GATC 2 cut(s) 5, 203
DpnII GATC 2 cut(s) 3, 201
Eco47I GGWCC 1 cut(s) 90
Eco57I CTGAAG 1 cut(s) 69
Eco88I CYCGRG 1 cut(s) 173
EcoO109I RGGNCCY 1 cut(s) 90
FaeI CATG 1 cut(s) 202
FaiI YATR 3 cut(s) 111, 200, 343
FatI CATG 1 cut(s) 198
FblI GTMKAC 1 cut(s) 342
Fnu4HI GCNGC 1 cut(s) 56
Fsp4HI GCNGC 1 cut(s) 56
FspBI CTAG 1 cut(s) 278
GlaI GCGC 1 cut(s) 54
GluI GCNGC 1 cut(s) 56
GsaI CCCAGC 1 cut(s) 285
HaeIII GGCC 1 cut(s) 135
HapII CCGG 1 cut(s) 137
HhaI GCGC 1 cut(s) 55
Hin1II CATG 1 cut(s) 202
Hin6I GCGC 1 cut(s) 53
HinP1I GCGC 1 cut(s) 53
HincII GTYRAC 1 cut(s) 238
HindII GTYRAC 1 cut(s) 238
HinfI GANTC 2 cut(s) 39, 208
HpaII CCGG 1 cut(s) 137
Hpy166II GTNNAC 4 cut(s) 22, 196, 238, 343
Hpy188I TCNGA 4 cut(s) 31, 105, 160, 328
Hpy188III TCNNGA 2 cut(s) 205, 372
Hpy8I GTNNAC 4 cut(s) 22, 196, 238, 343
HpyAV CCTTC 2 cut(s) 78, 222
HpyCH4III ACNGT 1 cut(s) 121
HpyF3I CTNAG 2 cut(s) 30, 102
Hsp92II CATG 1 cut(s) 202
HspAI GCGC 1 cut(s) 53
Kzo9I GATC 2 cut(s) 3, 201
LpnPI CCDG 3 cut(s) 150, 267, 290
Lsp1109I GCAGC 1 cut(s) 67
MaeI CTAG 1 cut(s) 278
MaeIII GTNAC 3 cut(s) 40, 121, 205
MalI GATC 2 cut(s) 5, 203
MboI GATC 2 cut(s) 3, 201
MluCI AATT 1 cut(s) 106
MlyI GAGTC 2 cut(s) 48, 202
MnlI CCTC 2 cut(s) 25, 226
MslI CAYNNNNRTG 1 cut(s) 203
MspA1I CMGCKG 1 cut(s) 47
MspI CCGG 1 cut(s) 137
MspR9I CCNGG 1 cut(s) 137
NciI CCSGG 1 cut(s) 137
NdeII GATC 2 cut(s) 3, 201
NlaIII CATG 1 cut(s) 202
NlaIV GGNNCC 1 cut(s) 91
NmuCI GTSAC 3 cut(s) 40, 121, 205
PkrI GCNGC 1 cut(s) 57
PleI GAGTC 2 cut(s) 47, 202
PpsI GAGTC 2 cut(s) 47, 202
PpuMI RGGWCCY 1 cut(s) 90
Psp5II RGGWCCY 1 cut(s) 90
PspFI CCCAGC 1 cut(s) 281
PspN4I GGNNCC 1 cut(s) 91
PspPI GGNCC 2 cut(s) 90, 134
PspPPI RGGWCCY 1 cut(s) 90
PvuII CAGCTG 1 cut(s) 47
RseI CAYNNNNRTG 1 cut(s) 203
SatI GCNGC 1 cut(s) 56
Sau3AI GATC 2 cut(s) 3, 201
Sau96I GGNCC 2 cut(s) 90, 134
SchI GAGTC 2 cut(s) 48, 202
ScrFI CCNGG 1 cut(s) 137
SetI ASST 7 cut(s) 36, 49, 60, 152, 237, 283, 358
SinI GGWCC 1 cut(s) 90
SmiMI CAYNNNNRTG 1 cut(s) 203
Sse9I AATT 1 cut(s) 106
SspMI CTAG 1 cut(s) 278
StyD4I CCNGG 1 cut(s) 135
TaaI ACNGT 1 cut(s) 121
TasI AATT 1 cut(s) 106
TseFI GTSAC 3 cut(s) 40, 121, 205
TseI GCWGC 1 cut(s) 55
Tsp45I GTSAC 3 cut(s) 40, 121, 205
TspDTI ATGAA 2 cut(s) 60, 285
VpaK11BI GGWCC 1 cut(s) 90
XmiI GTMKAC 1 cut(s) 342
XspI CTAG 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.