Prupe.5G085800_v2.0.a1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000302
Physical Location & Seq
Forward (+)
9751950 .. 9755426
3477 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G085800.1

Sequence Viewer

Length: 2277 bp
ATGGAGAACAAAAAGTGTGTGATGTTGCAGATTATATATCTTCTTGGCTTCAGTGTCATGTTTTATTTATCAGTAGCAGTTGCTGCGGAGACAGGTGAGGGCAAAAGTAGCATGCAAACTTACATTGTTTGGGTGAAAAGGCCAGTCCAGAACTTGTTTTTTGTAAAATCTCATGAAGATTTAGAGAGCTGGTATCACACATTTTTGCCGGACACTATTGCAAACTCAAACGAGCCGACAAAGTCAAGAATGGTTCATACATACCGCAATGTGGCCACTGGATTTGCAGCAAAACTGACGCCAGAGGAAGTAAAGGCAATGGAACAGAAAGAAGGGTTTGTGTCTGCTCATCCAGAGAGGATTCTGCCTTTGCACACTACTCATAGTCCTAACTTCTTGGGGTTGCAGCAAGGATTGGGAGTCTGGAAAGGAGCAAATTACGGTGAAGGTGTGATAATTGGAGTTTTGGATACAGGGATCGGACCGGATCATCCTTCATTTAGTGATGAAGGAGTACCGCCTCCTCCAGCTAAATGGAAAGGTAAGTGCGACTTCGATGGAACATTGTGCAATAACAAGCTTATTGGTGCAAGAAATTTCCAAAGCGGAAAAACTACAGGAGGCCCTCCAGTTGATGACGAAGGCCATGGCACCCACACATCCAGCACAGCTGCAGGGAATTTTGTGAAAGGAGCCAATGCTTTTGGAATGGCCAATGGCACAGCGGCTGGCATGGCACCTTATGCTCATTTGGCAATGTACAAAATCTGCTCTGAGGATGGTTGTACAGAAGGTGACATTGTAGCTGCTCTAGATACTGCTGTTGAAGATGGTGTGGACGTGCTCTCCCTTTCACTTGGTGGTCCTTCATTTCCTTTCTATGAAGATGGAATTGCAGTGGGTGCATTCGGAGCAATTCAAAAAGGAATTTTTGTCAGTTGTTCAGCTGGAAATTCTGGTCCTTCCTATGAGTCTTTATCCAATGAGGCCCCATGGATTCTCACAGTAGGAGCAAGCACCATTGACAGGAGCATAAGAGCAACAGCCTTGCTTGGAGATCATGGAGAATTTGACGGCGTATCTTTGTTCCAGCCTAAAGATTTTAATTCAACATTGTTACCTCTTGTTTATCCTGGTGCAAATGGCAACCCATCATCAGCTTTATGCAGTCCAGGATCCCTTGAAAATCTTGAAGGTAAAATAGTGGTGTGCGAAGGAGGAAGAGGAAGAGTTGCCAAAGGGGAAGAAGTGAAAAGAGCTGGTGGTGCTGCCATGATTCTTGTGAACCAAGCGATAGATGGCTATAGCGTCTTAGCTGATGCTCACGTCCTCCCTGCAACACATGTGAGTTATAAGGCAGGTTTGGATATCAAAGCCTTCATAAACTCAACCTCAACACCTACAGCCACAATCTTGTTTAAAGGCACTGTCATCGGAGATCCACTTGCTCCCAAGGTTGCTTCCTTCTCATCAAGAGGACCAAGCACTACAAGCCCTGGAATTTTGAAGCCTGACATCATTGGTCCCGGTGTTAGCATCCTAGCTGCATGGCCTGTTTCTGTAGACAATGGAACAGAAGGCAAGGCAACATTTAACATGGTTTCAGGTACCTCAATGTCATGCCCTCACTTAAGTGGCATTGCAGCCTTGCTCAAAAGCTCCCACCCTGACTGGTCACCAGCTGCCATTAAGTCAGCAATCATGACAACCGCTGAAGTAGAAAACCTCGCAGGCTCTGCCATTGTTGACCAAACACTTTTTCCAGCAGATATCTTTGCCCTTGGTGCAGGCCATGTTAACCCTTCAAAAGCAAATGACCCGGGGCTTATCTACGACATACAACCAGAGGATTACATTCCTTACTTGTGTGGTTTGAATTACACAGACGAGCAGATACAGGTCATCACCCAACAAACAGTGAACTGCTCTCAAGTAGGAGCCGTACCAGAAGCACAGCTAAATTATCCTACATTTTCTATCAAAACCGGGTCCTCAGAGACTAGGACTCAGTATTACACAAGAACGGTGACGAATGTTGGCCCAGCGAATTCAACTTACAACTTGGCTCTTGTAGTACCACCTAAAGTGGGCATGAGTGTAAATCCTCAGGTGCTCACATTCACAGAGTTTAACCAGAAAATTACCTACCATGTCGAGTTCAACGCACACGACGATGCTGGGAAGGACGGTGTCCCATTTGCTGAGGGATATTTGAGATGGGTTTCTGATAAGCATTCTGTTACCACTCGAATAGTTGCTACCTTCAGCACTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

759

Amino Acids

80.15

Weight (kDa)

5.36

Isoelectric Point (pI)

28.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1353
Acc36I ACCTGC 1 cut(s) 1349
Acc65I GGTACC 1 cut(s) 1607
AccB1I GGYRCC 3 cut(s) 650, 736, 1607
AccI GTMKAC 1 cut(s) 1563
AciI CCGC 6 cut(s) 86, 265, 518, 606, 725, 1710
AclWI GGATC 5 cut(s) 485, 495, 1170, 1183, 1433
AcoI YGGCCR 2 cut(s) 273, 711
AcsI RAATTY 7 cut(s) 595, 679, 927, 952, 1067, 1500, 2047
AcuI CTGAAG 3 cut(s) 34, 1734, 2248
AcyI GRCGYC 1 cut(s) 299
AdeI CACNNNGTG 1 cut(s) 860
AfaI GTAC 6 cut(s) 516, 761, 787, 1609, 1944, 2076
AfiI CCNNNNNNNGG 3 cut(s) 271, 1026, 2087
AflII CTTAAG 1 cut(s) 1630
AflIII ACRYGT 1 cut(s) 1342
AjiI CACGTC 2 cut(s) 841, 1327
AjnI CCWGG 3 cut(s) 1132, 1171, 1495
AjuI GAANNNNNNNTTGG 2 cut(s) 1743, 1775
AleI CACNNNNGTG 1 cut(s) 1632
Alw21I GWGCWC 2 cut(s) 846, 2115
Alw26I GTCTC 2 cut(s) 83, 1991
AlwI GGATC 5 cut(s) 485, 495, 1170, 1183, 1433
AlwNI CAGNNNCTG 3 cut(s) 83, 728, 1736
Ama87I CYCGRG 1 cut(s) 1819
AoxI GGCC 9 cut(s) 140, 273, 622, 643, 711, 987, 1550, 1789, 2038
ApeKI GCWGC 9 cut(s) 83, 287, 406, 671, 806, 1268, 1544, 1643, 1682
ApoI RAATTY 7 cut(s) 595, 679, 927, 952, 1067, 1500, 2047
Asp718I GGTACC 1 cut(s) 1607
AspS9I GGNCC 9 cut(s) 482, 623, 863, 959, 988, 1478, 1523, 1989, 2039
AsuC2I CCSGG 4 cut(s) 1527, 1820, 1821, 1987
AsuHPI GGTGA 7 cut(s) 107, 145, 455, 806, 1668, 1897, 2038
AvaI CYCGRG 1 cut(s) 1819
AvaII GGWCC 6 cut(s) 482, 863, 959, 1478, 1523, 1989
AxyI CCTNAGG 1 cut(s) 2106
BaeI ACNNNNGTAYC 2 cut(s) 807, 840
BalI TGGCCA 2 cut(s) 275, 713
BamHI GGATCC 1 cut(s) 1175
BanI GGYRCC 3 cut(s) 650, 736, 1607
Bbv12I GWGCWC 2 cut(s) 846, 2115
BbvCI CCTCAGC 1 cut(s) 2202
BbvI GCAGC 9 cut(s) 70, 299, 418, 658, 793, 1255, 1531, 1655, 1669
BccI CCATC 7 cut(s) 551, 773, 824, 881, 1159, 1292, 2211
BceAI ACGGC 2 cut(s) 1090, 1925
BcgI CGANNNNNNTGC 2 cut(s) 1414, 1448
BciT130I CCWGG 3 cut(s) 1134, 1173, 1497
BciVI GTATCC 1 cut(s) 463
BcnI CCSGG 4 cut(s) 1527, 1820, 1821, 1987
BcoDI GTCTC 2 cut(s) 83, 1991
BfaI CTAG 3 cut(s) 812, 1541, 2001
BfmI CTRYAG 5 cut(s) 615, 672, 1303, 1401, 1560
BfrI CTTAAG 1 cut(s) 1630
BfuAI ACCTGC 1 cut(s) 1349
BfuI GTATCC 1 cut(s) 463
Bme1390I CCNGG 7 cut(s) 1134, 1173, 1497, 1527, 1820, 1821, 1987
Bme18I GGWCC 6 cut(s) 482, 863, 959, 1478, 1523, 1989
BmeT110I CYCGRG 1 cut(s) 1819
BmgBI CACGTC 2 cut(s) 841, 1327
BmgT120I GGNCC 9 cut(s) 482, 623, 863, 959, 988, 1478, 1523, 1989, 2039
BmiI GGNNCC 9 cut(s) 652, 694, 738, 990, 1177, 1525, 1609, 1939, 1990
BmrFI CCNGG 7 cut(s) 1134, 1173, 1497, 1527, 1820, 1821, 1987
BmsI GCATC 3 cut(s) 1309, 1545, 2164
BpmI CTGGAG 2 cut(s) 510, 612
Bpu10I CCTNAGC 1 cut(s) 2202
BpuEI CTTGAG 1 cut(s) 1914
BpuMI CCSGG 4 cut(s) 1527, 1820, 1821, 1987
BsaHI GRCGYC 1 cut(s) 299
BsaJI CCNNGG 7 cut(s) 646, 992, 1452, 1495, 1780, 1819, 1820
BsaWI WCCGGW 1 cut(s) 484
BsaXI ACNNNNNCTCC 2 cut(s) 830, 860
Bsc4I CCNNNNNNNGG 3 cut(s) 271, 1026, 2087
Bse1I ACTGG 4 cut(s) 143, 283, 629, 1676
Bse21I CCTNAGG 1 cut(s) 2106
Bse3DI GCAATG 4 cut(s) 274, 324, 762, 1638
BseBI CCWGG 3 cut(s) 1134, 1173, 1497
BseDI CCNNGG 7 cut(s) 646, 992, 1452, 1495, 1780, 1819, 1820
BseGI GGATG 5 cut(s) 349, 490, 659, 784, 1536
BseLI CCNNNNNNNGG 3 cut(s) 271, 1026, 2087
BseMI GCAATG 4 cut(s) 274, 324, 762, 1638
BseMII CTCAG 5 cut(s) 765, 2007, 2021, 2120, 2193
BseNI ACTGG 4 cut(s) 143, 283, 629, 1676
BseRI GAGGAG 1 cut(s) 513
BseXI GCAGC 9 cut(s) 70, 299, 418, 658, 793, 1255, 1531, 1655, 1669
BseYI CCCAGC 2 cut(s) 2041, 2177
BsgI GTGCAG 1 cut(s) 1806
BshFI GGCC 9 cut(s) 142, 275, 624, 645, 713, 989, 1552, 1791, 2040
BshNI GGYRCC 3 cut(s) 650, 736, 1607
BsiHKAI GWGCWC 2 cut(s) 846, 2115
BsiHKCI CYCGRG 1 cut(s) 1819
BsiSI CCGG 5 cut(s) 209, 485, 1527, 1820, 1986
BslFI GGGAC 2 cut(s) 1509, 2177
BslI CCNNNNNNNGG 3 cut(s) 271, 1026, 2087
BsmAI GTCTC 2 cut(s) 83, 1991
BsmFI GGGAC 2 cut(s) 1509, 2177
BsmI GAATGC 2 cut(s) 905, 2233
BsnI GGCC 9 cut(s) 142, 275, 624, 645, 713, 989, 1552, 1791, 2040
BsoBI CYCGRG 1 cut(s) 1819
Bsp1286I GDGCHC 2 cut(s) 846, 2115
Bsp1407I TGTACA 2 cut(s) 759, 785
Bsp143I GATC 5 cut(s) 477, 487, 1057, 1175, 1438
Bsp19I CCATGG 2 cut(s) 646, 992
BspACI CCGC 6 cut(s) 86, 265, 518, 606, 725, 1710
BspANI GGCC 9 cut(s) 142, 275, 624, 645, 713, 989, 1552, 1791, 2040
BspCNI CTCAG 5 cut(s) 766, 2006, 2020, 2119, 2194
BspHI TCATGA 2 cut(s) 172, 1701
BspLI GGNNCC 9 cut(s) 652, 694, 738, 990, 1177, 1525, 1609, 1939, 1990
BspMAI CTGCAG 1 cut(s) 676
BspMI ACCTGC 1 cut(s) 1349
BspPI GGATC 5 cut(s) 485, 495, 1170, 1183, 1433
BspT107I GGYRCC 3 cut(s) 650, 736, 1607
BspTI CTTAAG 1 cut(s) 1630
BsrDI GCAATG 4 cut(s) 274, 324, 762, 1638
BsrGI TGTACA 2 cut(s) 759, 785
BsrI ACTGG 4 cut(s) 143, 283, 629, 1676
BssECI CCNNGG 7 cut(s) 646, 992, 1452, 1495, 1780, 1819, 1820
BssMI GATC 5 cut(s) 477, 487, 1057, 1175, 1438
BssNI GRCGYC 1 cut(s) 299
BssT1I CCWWGG 4 cut(s) 646, 992, 1452, 1780
Bst2UI CCWGG 3 cut(s) 1134, 1173, 1497
Bst4CI ACNGT 6 cut(s) 443, 1006, 1429, 1918, 2026, 2189
Bst6I CTCTTC 2 cut(s) 1216, 1222
BstACI GRCGYC 1 cut(s) 299
BstAFI CTTAAG 1 cut(s) 1630
BstAPI GCANNNNNTGC 4 cut(s) 83, 743, 902, 1736
BstAUI TGTACA 2 cut(s) 759, 785
BstC8I GCNNGC 5 cut(s) 113, 730, 1015, 1732, 1789
BstDEI CTNAG 6 cut(s) 774, 1312, 1993, 2007, 2106, 2202
BstDSI CCRYGG 2 cut(s) 646, 992
BstEII GGTNACC 1 cut(s) 1674
BstF5I GGATG 5 cut(s) 349, 490, 659, 784, 1536
BstKTI GATC 5 cut(s) 480, 490, 1060, 1178, 1441
BstMAI GTCTC 2 cut(s) 83, 1991
BstMBI GATC 5 cut(s) 477, 487, 1057, 1175, 1438
BstNI CCWGG 3 cut(s) 1134, 1173, 1497
BstNSI RCATGY 2 cut(s) 115, 1346
BstPI GGTNACC 1 cut(s) 1674
BstSCI CCNGG 7 cut(s) 1132, 1171, 1495, 1525, 1818, 1819, 1985
BstSFI CTRYAG 5 cut(s) 615, 672, 1303, 1401, 1560
BstV1I GCAGC 9 cut(s) 70, 299, 418, 658, 793, 1255, 1531, 1655, 1669
BstX2I RGATCY 2 cut(s) 1175, 1438
BstXI CCANNNNNNTGG 1 cut(s) 534
BstYI RGATCY 2 cut(s) 1175, 1438
Bsu36I CCTNAGG 1 cut(s) 2106
BsuI GTATCC 1 cut(s) 463
BsuRI GGCC 9 cut(s) 142, 275, 624, 645, 713, 989, 1552, 1791, 2040
BtgI CCRYGG 2 cut(s) 646, 992
BtrI CACGTC 2 cut(s) 841, 1327
BtsCI GGATG 5 cut(s) 349, 490, 659, 784, 1536
BtsI GCAGTG 1 cut(s) 903
BtsIMutI CAGTG 5 cut(s) 58, 276, 903, 1425, 1923
BveI ACCTGC 1 cut(s) 1349
Cac8I GCNNGC 5 cut(s) 113, 730, 1015, 1732, 1789
CaiI CAGNNNCTG 3 cut(s) 83, 728, 1736
CciI TCATGA 2 cut(s) 172, 1701
Cfr13I GGNCC 9 cut(s) 482, 623, 863, 959, 988, 1478, 1523, 1989, 2039
Cfr9I CCCGGG 1 cut(s) 1819
CpoI CGGWCCG 1 cut(s) 482
CseI GACGC 2 cut(s) 307, 1297
Csp6I GTAC 6 cut(s) 515, 760, 786, 1608, 1943, 2075
CspCI CAANNNNNGTGG 2 cut(s) 265, 300
CspI CGGWCCG 1 cut(s) 482
CviQI GTAC 6 cut(s) 515, 760, 786, 1608, 1943, 2075
DdeI CTNAG 6 cut(s) 774, 1312, 1993, 2007, 2106, 2202
DpnI GATC 5 cut(s) 479, 489, 1059, 1177, 1440
DpnII GATC 5 cut(s) 477, 487, 1057, 1175, 1438
DraI TTTAAA 1 cut(s) 1420
DraIII CACNNNGTG 1 cut(s) 860
EaeI YGGCCR 2 cut(s) 273, 711
Eam1104I CTCTTC 2 cut(s) 1216, 1222
EarI CTCTTC 2 cut(s) 1216, 1222
Eco130I CCWWGG 4 cut(s) 646, 992, 1452, 1780
Eco32I GATATC 2 cut(s) 1369, 1771
Eco47I GGWCC 6 cut(s) 482, 863, 959, 1478, 1523, 1989
Eco57I CTGAAG 3 cut(s) 34, 1734, 2248
Eco81I CCTNAGG 1 cut(s) 2106
Eco88I CYCGRG 1 cut(s) 1819
Eco91I GGTNACC 1 cut(s) 1674
EcoO109I RGGNCCY 3 cut(s) 623, 988, 1989
EcoO65I GGTNACC 1 cut(s) 1674
EcoRI GAATTC 1 cut(s) 2047
EcoRII CCWGG 3 cut(s) 1132, 1171, 1495
EcoRV GATATC 2 cut(s) 1369, 1771
EcoT14I CCWWGG 4 cut(s) 646, 992, 1452, 1780
ErhI CCWWGG 4 cut(s) 646, 992, 1452, 1780
FalI AAGNNNNNCTT 2 cut(s) 536, 568
FaqI GGGAC 2 cut(s) 1509, 2177
FblI GTMKAC 1 cut(s) 1563
FokI GGATG 5 cut(s) 336, 477, 646, 791, 1523
FspBI CTAG 3 cut(s) 812, 1541, 2001
GsaI CCCAGC 2 cut(s) 2045, 2181
GsuI CTGGAG 2 cut(s) 510, 612
HaeIII GGCC 9 cut(s) 142, 275, 624, 645, 713, 989, 1552, 1791, 2040
HapII CCGG 5 cut(s) 209, 485, 1527, 1820, 1986
HgaI GACGC 2 cut(s) 307, 1297
Hin1I GRCGYC 1 cut(s) 299
HincII GTYRAC 2 cut(s) 1747, 1798
HindII GTYRAC 2 cut(s) 1747, 1798
HindIII AAGCTT 1 cut(s) 578
HinfI GANTC 6 cut(s) 361, 420, 971, 997, 1276, 2005
HpaI GTTAAC 1 cut(s) 1798
HpaII CCGG 5 cut(s) 209, 485, 1527, 1820, 1986
HphI GGTGA 7 cut(s) 107, 145, 455, 806, 1668, 1897, 2038
Hpy166II GTNNAC 6 cut(s) 838, 1285, 1564, 1747, 1798, 1921
Hpy188I TCNGA 6 cut(s) 482, 775, 911, 1436, 1996, 2227
Hpy188III TCNNGA 9 cut(s) 148, 173, 246, 353, 424, 812, 1190, 1473, 1702
Hpy8I GTNNAC 6 cut(s) 838, 1285, 1564, 1747, 1798, 1921
Hpy99I CGWCG 1 cut(s) 2174
HpyCH4III ACNGT 6 cut(s) 443, 1006, 1429, 1918, 2026, 2189
HpyCH4IV ACGT 2 cut(s) 840, 1326
HpyF3I CTNAG 6 cut(s) 774, 1312, 1993, 2007, 2106, 2202
HpySE526I ACGT 2 cut(s) 840, 1326
Hsp92I GRCGYC 1 cut(s) 299
KpnI GGTACC 1 cut(s) 1611
KspAI GTTAAC 1 cut(s) 1798
Kzo9I GATC 5 cut(s) 477, 487, 1057, 1175, 1438
LmnI GCTCC 8 cut(s) 431, 692, 911, 1010, 1029, 1453, 1664, 1937
Lsp1109I GCAGC 9 cut(s) 70, 299, 418, 658, 793, 1255, 1531, 1655, 1669
LweI GCATC 3 cut(s) 1309, 1545, 2164
MaeI CTAG 3 cut(s) 812, 1541, 2001
MaeII ACGT 2 cut(s) 840, 1326
MaeIII GTNAC 5 cut(s) 794, 1116, 1674, 2026, 2239
MalI GATC 5 cut(s) 479, 489, 1059, 1177, 1440
MboI GATC 5 cut(s) 477, 487, 1057, 1175, 1438
MboII GAAGA 7 cut(s) 32, 188, 839, 896, 1233, 1239, 1256
MflI RGATCY 2 cut(s) 1175, 1438
MhlI GDGCHC 2 cut(s) 846, 2115
MlsI TGGCCA 2 cut(s) 275, 713
MluNI TGGCCA 2 cut(s) 275, 713
MlyI GAGTC 3 cut(s) 429, 980, 1999
Mox20I TGGCCA 2 cut(s) 275, 713
MscI TGGCCA 2 cut(s) 275, 713
MseI TTAA 7 cut(s) 1104, 1419, 1593, 1631, 1689, 1797, 2130
MslI CAYNNNNRTG 2 cut(s) 1632, 2172
Msp20I TGGCCA 2 cut(s) 275, 713
MspA1I CMGCKG 5 cut(s) 671, 725, 947, 1682, 1712
MspCI CTTAAG 1 cut(s) 1630
MspI CCGG 5 cut(s) 209, 485, 1527, 1820, 1986
MspR9I CCNGG 7 cut(s) 1134, 1173, 1497, 1527, 1820, 1821, 1987
Mva1269I GAATGC 2 cut(s) 905, 2233
MvaI CCWGG 3 cut(s) 1134, 1173, 1497
NciI CCSGG 4 cut(s) 1527, 1820, 1821, 1987
NcoI CCATGG 2 cut(s) 646, 992
NdeII GATC 5 cut(s) 477, 487, 1057, 1175, 1438
NlaIV GGNNCC 9 cut(s) 652, 694, 738, 990, 1177, 1525, 1609, 1939, 1990
NmuCI GTSAC 3 cut(s) 794, 1674, 2026
NspI RCATGY 2 cut(s) 115, 1346
OliI CACNNNNGTG 1 cut(s) 1632
PaeI GCATGC 1 cut(s) 115
PagI TCATGA 2 cut(s) 172, 1701
PciI ACATGT 1 cut(s) 1342
PcsI WCGNNNNNNNCGW 1 cut(s) 2169
PctI GAATGC 2 cut(s) 905, 2233
PfeI GAWTC 3 cut(s) 361, 997, 1276
PflFI GACNNNGTC 2 cut(s) 241, 2189
PfoI TCCNGGA 1 cut(s) 1171
PleI GAGTC 3 cut(s) 428, 979, 1999
PpsI GAGTC 3 cut(s) 428, 979, 1999
PpuMI RGGWCCY 1 cut(s) 1989
PscI ACATGT 1 cut(s) 1342
PsiI TTATAA 1 cut(s) 1353
Psp5II RGGWCCY 1 cut(s) 1989
Psp6I CCWGG 3 cut(s) 1132, 1171, 1495
PspEI GGTNACC 1 cut(s) 1674
PspFI CCCAGC 2 cut(s) 2041, 2177
PspGI CCWGG 3 cut(s) 1132, 1171, 1495
PspN4I GGNNCC 9 cut(s) 652, 694, 738, 990, 1177, 1525, 1609, 1939, 1990
PspPI GGNCC 9 cut(s) 482, 623, 863, 959, 988, 1478, 1523, 1989, 2039
PspPPI RGGWCCY 1 cut(s) 1989
PstI CTGCAG 1 cut(s) 676
PstNI CAGNNNCTG 3 cut(s) 83, 728, 1736
PsuI RGATCY 2 cut(s) 1175, 1438
PsyI GACNNNGTC 2 cut(s) 241, 2189
PvuII CAGCTG 3 cut(s) 671, 947, 1682
RsaI GTAC 6 cut(s) 516, 761, 787, 1609, 1944, 2076
RsaNI GTAC 6 cut(s) 515, 760, 786, 1608, 1943, 2075
RseI CAYNNNNRTG 2 cut(s) 1632, 2172
Rsr2I CGGWCCG 1 cut(s) 482
RsrII CGGWCCG 1 cut(s) 482
SaqAI TTAA 7 cut(s) 1104, 1419, 1593, 1631, 1689, 1797, 2130
Sau3AI GATC 5 cut(s) 477, 487, 1057, 1175, 1438
Sau96I GGNCC 9 cut(s) 482, 623, 863, 959, 988, 1478, 1523, 1989, 2039
SchI GAGTC 3 cut(s) 429, 980, 1999
ScrFI CCNGG 7 cut(s) 1134, 1173, 1497, 1527, 1820, 1821, 1987
SduI GDGCHC 2 cut(s) 846, 2115
SfaNI GCATC 3 cut(s) 1309, 1545, 2164
SfcI CTRYAG 5 cut(s) 615, 672, 1303, 1401, 1560
SinI GGWCC 6 cut(s) 482, 863, 959, 1478, 1523, 1989
SmaI CCCGGG 1 cut(s) 1821
SmiMI CAYNNNNRTG 2 cut(s) 1632, 2172
SmlI CTYRAG 2 cut(s) 1630, 1929
SmoI CTYRAG 2 cut(s) 1630, 1929
SphI GCATGC 1 cut(s) 115
SsiI CCGC 6 cut(s) 86, 265, 518, 606, 725, 1710
SspMI CTAG 3 cut(s) 812, 1541, 2001
StyD4I CCNGG 7 cut(s) 1132, 1171, 1495, 1525, 1818, 1819, 1985
StyI CCWWGG 4 cut(s) 646, 992, 1452, 1780
TaaI ACNGT 6 cut(s) 443, 1006, 1429, 1918, 2026, 2189
TaiI ACGT 2 cut(s) 843, 1329
TaqI TCGA 3 cut(s) 555, 2154, 2248
TatI WGTACW 2 cut(s) 759, 785
TauI GCSGC 1 cut(s) 728
TfiI GAWTC 3 cut(s) 361, 997, 1276
Tru1I TTAA 7 cut(s) 1104, 1419, 1593, 1631, 1689, 1797, 2130
Tru9I TTAA 7 cut(s) 1104, 1419, 1593, 1631, 1689, 1797, 2130
TscAI CASTG 5 cut(s) 58, 283, 903, 1432, 1923
TseFI GTSAC 3 cut(s) 794, 1674, 2026
TseI GCWGC 9 cut(s) 83, 287, 406, 671, 806, 1268, 1544, 1643, 1682
Tsp45I GTSAC 3 cut(s) 794, 1674, 2026
TspDTI ATGAA 7 cut(s) 189, 245, 486, 522, 858, 897, 1369
TspMI CCCGGG 1 cut(s) 1819
TspRI CASTG 5 cut(s) 58, 283, 903, 1432, 1923
Tth111I GACNNNGTC 2 cut(s) 241, 2189
Vha464I CTTAAG 1 cut(s) 1630
VpaK11BI GGWCC 6 cut(s) 482, 863, 959, 1478, 1523, 1989
XapI RAATTY 7 cut(s) 595, 679, 927, 952, 1067, 1500, 2047
XbaI TCTAGA 1 cut(s) 811
XceI RCATGY 2 cut(s) 115, 1346
XcmI CCANNNNNNNNNTGG 1 cut(s) 1295
XmaI CCCGGG 1 cut(s) 1819
XmiI GTMKAC 1 cut(s) 1563
XspI CTAG 3 cut(s) 812, 1541, 2001
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.