Rh7AG183800

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000302
Physical Location & Seq
Reverse (-)
16324477 .. 16330709
6233 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG183800.1

Sequence Viewer

Length: 966 bp
ATGGAGAACAAAAAGTGTGTAGTGATGCAGATCATATACCTTGGCTTTAGTTGCATGTTTTACTTGTCACTAGCAGTTGCTGTTGAAAAAGTGGCAGTACTGGAACCACAAGGTGAGACAGATAGCATGCAAACTTATATAGTTTTGGTTGAAAGGCCACCCAGCAATTTCAATGCACAATCTGATCATGAAGATTTAGAGAGTTGGTACCAGTCATTTCTGCCCCAAACTATTGCAAACTCAAACCAGCTCATAAAGCCACGAATGGTTTATGCATACCACAATGTGGCCACAGGATTTGCAGCAAAGCTGACTGGGGAGGAAGTTGAAGAAATGGAGAAGAAAGATGGGTTTATCTATGCTCATCCAGAACGGAATCTGCAATTGCACACAACTCATAGTCCTAACTTCTTGGGGCTGCAACAAGGATTGGGACTTTGGAAAGGGTCTAATTATGGTGACCATAGCCATGATTCATCAGCTCTTTGCGATGTAGGGTCCCTTACAAATGTTGAAGGGAAAGTAGTGGTGTGTGAGCTAGGTGGCGGAATTGGAAGAATTGCAAAAGGGGTAGAAGTGAAAAGAGCTGGTGGTGTTGCCATGATTCTTGTCAACCCAGACTTTGCTGGCTATACCACCATAGCAGATGCTCACGTGCTTCCGGCAACACATGTGAGTTACTCTGCAGGGCACTGGCTCAAGAGCTCACACCCTAACTGGTCGCCGGCTGCCATTAAGTCTGCAATCAAGACAACCGCTGATGTACTAAACCTCGGAGGCAAGCCCATTGTTGATGAAAGACATGCTCCAGCAGACATCTTTGCCACTGGTGCAGGCCATGTCAAACCTTCAAAAGCAAATGACCTTGGGCTCATCTACGACCTAAACCCTGAAGATTACATCCCCTACTTGTGCGGTTTGAATTATACAGATGACCAGATACTGATGTTACCCAAGAAACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

321

Amino Acids

35.06

Weight (kDa)

5.68

Isoelectric Point (pI)

34.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 45 - 130 1.1e-11 Peptidase inhibitor I9
PA PF02225 162 - 233 1.5e-10 PA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 207
AccB1I GGYRCC 1 cut(s) 207
AccB7I CCANNNNNTGG 1 cut(s) 286
AciI CCGC 3 cut(s) 546, 756, 915
AcoI YGGCCR 1 cut(s) 288
AcuI CTGAAG 1 cut(s) 912
AcvI CACGTG 1 cut(s) 655
AdeI CACNNNGTG 2 cut(s) 113, 286
AfaI GTAC 3 cut(s) 99, 209, 765
AfiI CCNNNNNNNGG 1 cut(s) 286
AflIII ACRYGT 1 cut(s) 670
AgsI TTSAA 7 cut(s) 86, 152, 172, 329, 515, 852, 922
AluBI AGCT 6 cut(s) 250, 310, 482, 538, 587, 705
AluI AGCT 6 cut(s) 250, 310, 482, 538, 587, 705
Alw21I GWGCWC 1 cut(s) 707
Alw26I GTCTC 1 cut(s) 110
AlwNI CAGNNNCTG 2 cut(s) 80, 943
AoxI GGCC 3 cut(s) 155, 288, 835
ApeKI GCWGC 3 cut(s) 302, 418, 728
Asp718I GGTACC 1 cut(s) 207
AspS9I GGNCC 1 cut(s) 498
AsuHPI GGTGA 2 cut(s) 125, 470
AvaII GGWCC 1 cut(s) 498
BaeGI GKGCMC 1 cut(s) 693
BaeI ACNNNNGTAYC 2 cut(s) 932, 965
BalI TGGCCA 1 cut(s) 290
BanI GGYRCC 1 cut(s) 207
BanII GRGCYC 2 cut(s) 707, 873
BbrPI CACGTG 1 cut(s) 655
Bbv12I GWGCWC 1 cut(s) 707
BbvI GCAGC 3 cut(s) 314, 405, 715
BccI CCATC 1 cut(s) 341
BclI TGATCA 1 cut(s) 184
BcoDI GTCTC 1 cut(s) 110
BfaI CTAG 2 cut(s) 71, 539
BfmI CTRYAG 1 cut(s) 684
BisI GCNGC 3 cut(s) 303, 419, 729
BlsI GCNGC 3 cut(s) 304, 420, 730
BmcAI AGTACT 1 cut(s) 99
Bme18I GGWCC 1 cut(s) 498
BmgT120I GGNCC 1 cut(s) 498
BmiI GGNNCC 4 cut(s) 105, 209, 499, 500
BmrI ACTGGG 1 cut(s) 324
BmsI GCATC 2 cut(s) 15, 637
BmuI ACTGGG 1 cut(s) 324
BpmI CTGGAG 1 cut(s) 792
BpuEI CTTGAG 1 cut(s) 683
BsaAI YACGTR 1 cut(s) 655
BsaBI GATNNNNATC 1 cut(s) 29
BsaJI CCNNGG 3 cut(s) 40, 772, 865
Bsc4I CCNNNNNNNGG 1 cut(s) 286
Bse118I RCCGGY 1 cut(s) 724
Bse1I ACTGG 6 cut(s) 105, 211, 319, 698, 722, 832
Bse8I GATNNNNATC 1 cut(s) 29
BseDI CCNNGG 3 cut(s) 40, 772, 865
BseGI GGATG 2 cut(s) 364, 900
BseJI GATNNNNATC 1 cut(s) 29
BseLI CCNNNNNNNGG 1 cut(s) 286
BseNI ACTGG 6 cut(s) 105, 211, 319, 698, 722, 832
BseSI GKGCMC 1 cut(s) 693
BseXI GCAGC 3 cut(s) 314, 405, 715
BseYI CCCAGC 1 cut(s) 161
BsgI GTGCAG 1 cut(s) 852
BshFI GGCC 3 cut(s) 157, 290, 837
BshNI GGYRCC 1 cut(s) 207
BsiHKAI GWGCWC 1 cut(s) 707
BsiSI CCGG 2 cut(s) 662, 725
BslFI GGGAC 2 cut(s) 447, 484
BslI CCNNNNNNNGG 1 cut(s) 286
BsmAI GTCTC 1 cut(s) 110
BsmFI GGGAC 2 cut(s) 447, 484
BsnI GGCC 3 cut(s) 157, 290, 837
Bsp1286I GDGCHC 3 cut(s) 693, 707, 873
Bsp143I GATC 2 cut(s) 30, 184
BspACI CCGC 3 cut(s) 546, 756, 915
BspANI GGCC 3 cut(s) 157, 290, 837
BspHI TCATGA 1 cut(s) 187
BspLI GGNNCC 4 cut(s) 105, 209, 499, 500
BspMAI CTGCAG 1 cut(s) 688
BspT107I GGYRCC 1 cut(s) 207
BsrFI RCCGGY 1 cut(s) 724
BsrI ACTGG 6 cut(s) 105, 211, 319, 698, 722, 832
BssAI RCCGGY 1 cut(s) 724
BssECI CCNNGG 3 cut(s) 40, 772, 865
BssMI GATC 2 cut(s) 30, 184
BssT1I CCWWGG 2 cut(s) 40, 865
Bst4CI ACNGT 1 cut(s) 963
BstBAI YACGTR 1 cut(s) 655
BstC8I GCNNGC 5 cut(s) 128, 628, 726, 782, 835
BstEII GGTNACC 1 cut(s) 458
BstF5I GGATG 2 cut(s) 364, 900
BstKTI GATC 2 cut(s) 33, 187
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 2 cut(s) 30, 184
BstMWI GCNNNNNNNGC 3 cut(s) 51, 256, 830
BstNSI RCATGY 4 cut(s) 58, 130, 674, 806
BstPI GGTNACC 1 cut(s) 458
BstSFI CTRYAG 1 cut(s) 684
BstSLI GKGCMC 1 cut(s) 693
BstV1I GCAGC 3 cut(s) 314, 405, 715
BsuRI GGCC 3 cut(s) 157, 290, 837
BtgZI GCGATG 1 cut(s) 504
BtsCI GGATG 2 cut(s) 364, 900
BtsIMutI CAGTG 2 cut(s) 691, 825
Cac8I GCNNGC 5 cut(s) 128, 628, 726, 782, 835
CaiI CAGNNNCTG 2 cut(s) 80, 943
CciI TCATGA 1 cut(s) 187
Cfr10I RCCGGY 1 cut(s) 724
Cfr13I GGNCC 1 cut(s) 498
Csp6I GTAC 3 cut(s) 98, 208, 764
CspCI CAANNNNNGTGG 2 cut(s) 280, 315
CviAII CATG 8 cut(s) 55, 127, 188, 470, 601, 671, 803, 839
CviQI GTAC 3 cut(s) 98, 208, 764
DpnI GATC 2 cut(s) 32, 186
DpnII GATC 2 cut(s) 30, 184
DraIII CACNNNGTG 2 cut(s) 113, 286
EaeI YGGCCR 1 cut(s) 288
EciI GGCGGA 1 cut(s) 561
Ecl136II GAGCTC 1 cut(s) 705
Eco130I CCWWGG 2 cut(s) 40, 865
Eco24I GRGCYC 2 cut(s) 707, 873
Eco47I GGWCC 1 cut(s) 498
Eco53kI GAGCTC 1 cut(s) 705
Eco57I CTGAAG 1 cut(s) 912
Eco72I CACGTG 1 cut(s) 655
Eco91I GGTNACC 1 cut(s) 458
EcoICRI GAGCTC 1 cut(s) 705
EcoO109I RGGNCCY 1 cut(s) 498
EcoO65I GGTNACC 1 cut(s) 458
EcoT14I CCWWGG 2 cut(s) 40, 865
EcoT22I ATGCAT 1 cut(s) 277
EcoT38I GRGCYC 2 cut(s) 707, 873
ErhI CCWWGG 2 cut(s) 40, 865
FaeI CATG 8 cut(s) 58, 130, 191, 473, 604, 674, 806, 842
FaqI GGGAC 2 cut(s) 447, 484
FatI CATG 8 cut(s) 54, 126, 187, 469, 600, 670, 802, 838
FbaI TGATCA 1 cut(s) 184
Fnu4HI GCNGC 3 cut(s) 303, 419, 729
FokI GGATG 2 cut(s) 351, 887
FriOI GRGCYC 2 cut(s) 707, 873
Fsp4HI GCNGC 3 cut(s) 303, 419, 729
FspBI CTAG 2 cut(s) 71, 539
GluI GCNGC 3 cut(s) 303, 419, 729
GsaI CCCAGC 1 cut(s) 165
GsuI CTGGAG 1 cut(s) 792
HaeIII GGCC 3 cut(s) 157, 290, 837
HapII CCGG 2 cut(s) 662, 725
Hin1II CATG 8 cut(s) 58, 130, 191, 473, 604, 674, 806, 842
HincII GTYRAC 1 cut(s) 613
HindII GTYRAC 1 cut(s) 613
HinfI GANTC 3 cut(s) 376, 473, 604
HpaII CCGG 2 cut(s) 662, 725
HphI GGTGA 2 cut(s) 125, 470
Hpy166II GTNNAC 1 cut(s) 613
Hpy188I TCNGA 2 cut(s) 184, 776
Hpy188III TCNNGA 4 cut(s) 188, 368, 700, 748
Hpy8I GTNNAC 1 cut(s) 613
HpyAV CCTTC 2 cut(s) 509, 858
HpyCH4III ACNGT 1 cut(s) 963
HpyCH4IV ACGT 1 cut(s) 654
HpyF10VI GCNNNNNNNGC 3 cut(s) 51, 256, 830
HpySE526I ACGT 1 cut(s) 654
Hsp92II CATG 8 cut(s) 58, 130, 191, 473, 604, 674, 806, 842
KflI GGGWCCC 1 cut(s) 498
KpnI GGTACC 1 cut(s) 211
KroI GCCGGC 1 cut(s) 724
KroNI GCCGGC 1 cut(s) 726
Ksp22I TGATCA 1 cut(s) 184
Kzo9I GATC 2 cut(s) 30, 184
LmnI GCTCC 1 cut(s) 811
Lsp1109I GCAGC 3 cut(s) 314, 405, 715
LweI GCATC 2 cut(s) 15, 637
MaeI CTAG 2 cut(s) 71, 539
MaeII ACGT 1 cut(s) 654
MaeIII GTNAC 4 cut(s) 66, 458, 677, 948
MalI GATC 2 cut(s) 32, 186
MboI GATC 2 cut(s) 30, 184
MboII GAAGA 5 cut(s) 203, 341, 352, 567, 905
MfeI CAATTG 1 cut(s) 383
MhlI GDGCHC 3 cut(s) 693, 707, 873
MlsI TGGCCA 1 cut(s) 290
MluCI AATT 6 cut(s) 166, 383, 451, 549, 558, 922
MluNI TGGCCA 1 cut(s) 290
MnlI CCTC 3 cut(s) 313, 770, 782
Mox20I TGGCCA 1 cut(s) 290
Mph1103I ATGCAT 1 cut(s) 277
MroNI GCCGGC 1 cut(s) 724
MscI TGGCCA 1 cut(s) 290
MseI TTAA 1 cut(s) 735
MslI CAYNNNNRTG 1 cut(s) 468
Msp20I TGGCCA 1 cut(s) 290
MspA1I CMGCKG 1 cut(s) 758
MspI CCGG 2 cut(s) 662, 725
MunI CAATTG 1 cut(s) 383
MwoI GCNNNNNNNGC 3 cut(s) 51, 256, 830
NaeI GCCGGC 1 cut(s) 726
NdeII GATC 2 cut(s) 30, 184
NgoMIV GCCGGC 1 cut(s) 724
NlaIII CATG 8 cut(s) 58, 130, 191, 473, 604, 674, 806, 842
NlaIV GGNNCC 4 cut(s) 105, 209, 499, 500
NmuCI GTSAC 2 cut(s) 66, 458
NsiI ATGCAT 1 cut(s) 277
NspI RCATGY 4 cut(s) 58, 130, 674, 806
PaeI GCATGC 1 cut(s) 130
PagI TCATGA 1 cut(s) 187
PciI ACATGT 1 cut(s) 670
PdiI GCCGGC 1 cut(s) 726
PfeI GAWTC 3 cut(s) 376, 473, 604
PflMI CCANNNNNTGG 1 cut(s) 286
PkrI GCNGC 3 cut(s) 304, 420, 730
PmaCI CACGTG 1 cut(s) 655
PmlI CACGTG 1 cut(s) 655
Ppu21I YACGTR 1 cut(s) 655
PpuMI RGGWCCY 1 cut(s) 498
PscI ACATGT 1 cut(s) 670
Psp124BI GAGCTC 1 cut(s) 707
Psp5II RGGWCCY 1 cut(s) 498
PspCI CACGTG 1 cut(s) 655
PspEI GGTNACC 1 cut(s) 458
PspFI CCCAGC 1 cut(s) 161
PspN4I GGNNCC 4 cut(s) 105, 209, 499, 500
PspPI GGNCC 1 cut(s) 498
PspPPI RGGWCCY 1 cut(s) 498
PstI CTGCAG 1 cut(s) 688
PstNI CAGNNNCTG 2 cut(s) 80, 943
RsaI GTAC 3 cut(s) 99, 209, 765
RsaNI GTAC 3 cut(s) 98, 208, 764
RseI CAYNNNNRTG 1 cut(s) 468
SacI GAGCTC 1 cut(s) 707
SaqAI TTAA 1 cut(s) 735
SatI GCNGC 3 cut(s) 303, 419, 729
Sau3AI GATC 2 cut(s) 30, 184
Sau96I GGNCC 1 cut(s) 498
ScaI AGTACT 1 cut(s) 99
SduI GDGCHC 3 cut(s) 693, 707, 873
SfaNI GCATC 2 cut(s) 15, 637
SfcI CTRYAG 1 cut(s) 684
SinI GGWCC 1 cut(s) 498
SmiMI CAYNNNNRTG 1 cut(s) 468
SmlI CTYRAG 1 cut(s) 698
SmoI CTYRAG 1 cut(s) 698
SphI GCATGC 1 cut(s) 130
Sse9I AATT 6 cut(s) 166, 383, 451, 549, 558, 922
SsiI CCGC 3 cut(s) 546, 756, 915
SspMI CTAG 2 cut(s) 71, 539
SstI GAGCTC 1 cut(s) 707
StyI CCWWGG 2 cut(s) 40, 865
TaaI ACNGT 1 cut(s) 963
TaiI ACGT 1 cut(s) 657
TasI AATT 6 cut(s) 166, 383, 451, 549, 558, 922
TatI WGTACW 2 cut(s) 97, 763
TfiI GAWTC 3 cut(s) 376, 473, 604
Tru1I TTAA 1 cut(s) 735
Tru9I TTAA 1 cut(s) 735
TscAI CASTG 2 cut(s) 698, 832
TseFI GTSAC 2 cut(s) 66, 458
TseI GCWGC 3 cut(s) 302, 418, 728
Tsp45I GTSAC 2 cut(s) 66, 458
TspDTI ATGAA 3 cut(s) 204, 465, 810
TspGWI ACGGA 1 cut(s) 388
TspRI CASTG 2 cut(s) 698, 832
Van91I CCANNNNNTGG 1 cut(s) 286
VpaK11BI GGWCC 1 cut(s) 498
XceI RCATGY 4 cut(s) 58, 130, 674, 806
XspI CTAG 2 cut(s) 71, 539
ZrmI AGTACT 1 cut(s) 99
Zsp2I ATGCAT 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.