Rroxscaffold_3G00256270

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000302
Physical Location & Seq
Forward (+)
50638838 .. 50640361
1524 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00256270.1

Sequence Viewer

Length: 1524 bp
ATGTACCAAGTTTGTTCTGAACAGGGTTGTTCTGAAAGTGACATTGTAGCTGCTATGGACACTGCTGTTGCCGATGGAGTGGACGTGCTCTCCCTCTCACTTGGTGGTGGCTCAGCTCCTTTCTATGCAGACGGAATCGCAGTCGGTGCATTTGGAGCAATTCAGAAGGGAATTTTTGTTAGCTGCTCAGCTGGAAATGAGGGTCCTGTATATGGTTCTTTATCCAATGAAGCGCCATGGATTCTAACCGTCGGAGCAAGCACCATTGACAGAAGCATAAAGGCAACAGCAAAACTTGGAAATGGGCAGGAATATGATGGGGAATCGTTATTCCAGGATAAAAATTTTAGTTCAAATTTGTTACCTCTTGTTTATGCCGGTGCATATAGCAATGATTCAACAGCTCTGTGCGATGTAGGAACCCTTAATGGAGTTGAAGGGAAAGTGGTGTTGTGTGAAAGAGGTGGAGGAATTGGAAGAATTGCCAAAGGAGAAGAAGTCAAAAGAGCAGGTGGTGCTGCCATGATCCTCATGAACGAAGACACTGATGGCTATAGCACCTTAGCTGATGCTCATGTGCTTCCTGCAACACATGTGAGTTATGCTGCAGGTTTGAGCATCAAAGCCTACATAAACTCCACCTCAACACCTATGGCCACAATCTTGTTCAAAGGCACTGTCATCGGTGATCAAAATGCTCCCGCAGTCACTTCCTTTTCATCAAGAGGACCAAGCCTGGCGAGCACAGGAATTTTGAAACCTGACATCATTGGTCCCGGTGTTAGCATCTTAGCTGCATGGCCTGTTTCGGTGGACAATGGCACAGAATCTACGGCAACATTTAATATGGTTTCAGGTACTTCAATGTCATGCCCTCACCTGAGTGGCATTGCAGCTTTGCTCAAGTGCTCACACCCCGACTGGTCACCAGCTGCCATCAAGTCTGCAATTGTGACAACCGCTGACATAGTAAACCTCGGAGGCAAACCTATTATTGATGAAACACTTGCTCCAGCAGACATCTTTGCCACTGGTGCAGGACATGTTAATCCTCCAAAAGCAGATGACCCAGGGCTAATCTACGACACAAAACCAGTGGATTACATTCCCTACCTGTGCGGTTTGAACTACACAGACGATCAGATACAGATAATCACCCAACAAACAGTGAAGTGCTCTGAAGTAGGAGTCATACCAGAAGCGCAGCTAAATTATCCTTCATTTTCTATTATATTCCAGGACTTGTCTCAGTCTCAAGATTACACAAGGACGGTGACAAATGTTGGACCAGCTAATTCAACTTACAACTTGGATCTTAAGTTACCAGGTAAAATCGGCATGAGTGTGAGTCCTTCGCAGCTTAAGTTTACAGAAGTTGGCCAGACAATTACATACCTAGCAGAGTTTATCCCGGAAGATGGAGCAGGGAAGGATGCACCATTTTATCAGGGATATTTGAGATGGTTCTCTGATCAGCATTCTGTTACTAGCCCCATATCTGTGATCTTCGACACCCAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

507

Amino Acids

52.91

Weight (kDa)

4.46

Isoelectric Point (pI)

30.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 2 - 324 3.5e-30 Subtilase family
PA PF02225 122 - 207 8e-14 PA domain
fn3_6 PF17766 402 - 501 6.1e-24 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 500
Acc36I ACCTGC 2 cut(s) 500, 599
AciI CCGC 3 cut(s) 702, 960, 1119
AclWI GGATC 2 cut(s) 520, 1320
AcoI YGGCCR 2 cut(s) 654, 1378
AcsI RAATTY 4 cut(s) 171, 343, 355, 750
AcuI CTGAAG 1 cut(s) 1200
AdeI CACNNNGTG 1 cut(s) 104
AfaI GTAC 2 cut(s) 5, 859
AfiI CCNNNNNNNGG 2 cut(s) 212, 1418
AflII CTTAAG 2 cut(s) 1316, 1361
AflIII ACRYGT 2 cut(s) 592, 1042
AgsI TTSAA 8 cut(s) 354, 399, 437, 670, 757, 864, 1126, 1299
AjiI CACGTC 1 cut(s) 85
AjnI CCWGG 5 cut(s) 333, 735, 1069, 1236, 1324
AleI CACNNNNGTG 1 cut(s) 882
Alw21I GWGCWC 4 cut(s) 90, 746, 911, 1178
Alw26I GTCTC 2 cut(s) 1251, 1257
AlwI GGATC 2 cut(s) 520, 1320
AoxI GGCC 3 cut(s) 654, 800, 1378
ApeKI GCWGC 9 cut(s) 50, 183, 518, 605, 794, 893, 932, 1204, 1357
ApoI RAATTY 4 cut(s) 171, 343, 355, 750
ArsI GACNNNNNNTTYG 2 cut(s) 663, 695
AspLEI GCGC 2 cut(s) 235, 1204
AspS9I GGNCC 4 cut(s) 203, 728, 773, 1286
AsuC2I CCSGG 2 cut(s) 777, 1412
AsuHPI GGTGA 5 cut(s) 698, 869, 918, 1147, 1285
AvaII GGWCC 4 cut(s) 203, 728, 773, 1286
BalI TGGCCA 2 cut(s) 656, 1380
BbsI GAAGAC 1 cut(s) 546
Bbv12I GWGCWC 4 cut(s) 90, 746, 911, 1178
BbvI GCAGC 9 cut(s) 37, 170, 505, 592, 781, 905, 919, 1216, 1369
BccI CCATC 6 cut(s) 68, 311, 542, 944, 1412, 1455
BceAI ACGGC 1 cut(s) 849
BcgI CGANNNNNNTGC 2 cut(s) 664, 698
BciT130I CCWGG 5 cut(s) 335, 737, 1071, 1238, 1326
BclI TGATCA 2 cut(s) 688, 1471
BcnI CCSGG 2 cut(s) 777, 1412
BcoDI GTCTC 2 cut(s) 1251, 1257
BfaI CTAG 2 cut(s) 1397, 1488
BfmI CTRYAG 2 cut(s) 553, 606
BfoI RGCGCY 1 cut(s) 236
BfrI CTTAAG 2 cut(s) 1316, 1361
BfuAI ACCTGC 2 cut(s) 500, 599
BisI GCNGC 9 cut(s) 51, 184, 519, 606, 795, 894, 933, 1205, 1358
BlpI GCTNAGC 2 cut(s) 112, 187
BlsI GCNGC 9 cut(s) 52, 185, 520, 607, 796, 895, 934, 1206, 1359
Bme1390I CCNGG 7 cut(s) 335, 737, 777, 1071, 1238, 1326, 1412
Bme18I GGWCC 4 cut(s) 203, 728, 773, 1286
BmgBI CACGTC 1 cut(s) 85
BmgT120I GGNCC 4 cut(s) 203, 728, 773, 1286
BmiI GGNNCC 3 cut(s) 204, 421, 775
BmrFI CCNGG 7 cut(s) 335, 737, 777, 1071, 1238, 1326, 1412
BmsI GCATC 4 cut(s) 559, 627, 795, 1423
BpiI GAAGAC 1 cut(s) 546
BpmI CTGGAG 1 cut(s) 996
Bpu10I CCTNAGC 1 cut(s) 562
Bpu1102I GCTNAGC 2 cut(s) 112, 187
BpuEI CTTGAG 2 cut(s) 887, 1239
BpuMI CCSGG 2 cut(s) 777, 1412
BsaJI CCNNGG 4 cut(s) 236, 976, 1069, 1070
Bsc4I CCNNNNNNNGG 2 cut(s) 212, 1418
Bse118I RCCGGY 1 cut(s) 377
Bse1I ACTGG 3 cut(s) 926, 1036, 1094
Bse3DI GCAATG 2 cut(s) 397, 888
BseBI CCWGG 5 cut(s) 335, 737, 1071, 1238, 1326
BseDI CCNNGG 4 cut(s) 236, 976, 1069, 1070
BseGI GGATG 1 cut(s) 1438
BseLI CCNNNNNNNGG 2 cut(s) 212, 1418
BseMI GCAATG 2 cut(s) 397, 888
BseMII CTCAG 4 cut(s) 126, 201, 872, 1262
BseNI ACTGG 3 cut(s) 926, 1036, 1094
BseXI GCAGC 9 cut(s) 37, 170, 505, 592, 781, 905, 919, 1216, 1369
BseYI CCCAGC 1 cut(s) 1515
BsgI GTGCAG 1 cut(s) 1056
BshFI GGCC 3 cut(s) 656, 802, 1380
BsiHKAI GWGCWC 4 cut(s) 90, 746, 911, 1178
BsiSI CCGG 3 cut(s) 378, 777, 1412
BslFI GGGAC 1 cut(s) 759
BslI CCNNNNNNNGG 2 cut(s) 212, 1418
BsmAI GTCTC 2 cut(s) 1251, 1257
BsmFI GGGAC 1 cut(s) 759
BsmI GAATGC 1 cut(s) 1477
BsnI GGCC 3 cut(s) 656, 802, 1380
Bsp1286I GDGCHC 4 cut(s) 90, 746, 911, 1178
Bsp143I GATC 6 cut(s) 525, 688, 1138, 1312, 1471, 1503
Bsp1720I GCTNAGC 2 cut(s) 112, 187
Bsp19I CCATGG 1 cut(s) 236
BspACI CCGC 3 cut(s) 702, 960, 1119
BspANI GGCC 3 cut(s) 656, 802, 1380
BspCNI CTCAG 4 cut(s) 125, 200, 873, 1261
BspHI TCATGA 1 cut(s) 531
BspLI GGNNCC 3 cut(s) 204, 421, 775
BspMAI CTGCAG 1 cut(s) 610
BspMI ACCTGC 2 cut(s) 500, 599
BspPI GGATC 2 cut(s) 520, 1320
BspTI CTTAAG 2 cut(s) 1316, 1361
BsrDI GCAATG 2 cut(s) 397, 888
BsrFI RCCGGY 1 cut(s) 377
BsrI ACTGG 3 cut(s) 926, 1036, 1094
BssAI RCCGGY 1 cut(s) 377
BssECI CCNNGG 4 cut(s) 236, 976, 1069, 1070
BssMI GATC 6 cut(s) 525, 688, 1138, 1312, 1471, 1503
BssT1I CCWWGG 1 cut(s) 236
Bst2UI CCWGG 5 cut(s) 335, 737, 1071, 1238, 1326
Bst4CI ACNGT 4 cut(s) 250, 679, 1168, 1273
BstAFI CTTAAG 2 cut(s) 1316, 1361
BstAPI GCANNNNNTGC 2 cut(s) 146, 515
BstC8I GCNNGC 2 cut(s) 259, 742
BstDEI CTNAG 6 cut(s) 112, 187, 562, 790, 881, 1248
BstDSI CCRYGG 1 cut(s) 236
BstEII GGTNACC 1 cut(s) 924
BstF5I GGATG 1 cut(s) 1438
BstH2I RGCGCY 1 cut(s) 236
BstHHI GCGC 2 cut(s) 235, 1204
BstKTI GATC 6 cut(s) 528, 691, 1141, 1315, 1474, 1506
BstMAI GTCTC 2 cut(s) 1251, 1257
BstMBI GATC 6 cut(s) 525, 688, 1138, 1312, 1471, 1503
BstMWI GCNNNNNNNGC 5 cut(s) 146, 155, 515, 741, 1034
BstNI CCWGG 5 cut(s) 335, 737, 1071, 1238, 1326
BstNSI RCATGY 2 cut(s) 596, 1046
BstPI GGTNACC 1 cut(s) 924
BstSCI CCNGG 7 cut(s) 333, 735, 775, 1069, 1236, 1324, 1410
BstSFI CTRYAG 2 cut(s) 553, 606
BstV1I GCAGC 9 cut(s) 37, 170, 505, 592, 781, 905, 919, 1216, 1369
BstV2I GAAGAC 1 cut(s) 546
BstX2I RGATCY 1 cut(s) 1312
BstYI RGATCY 1 cut(s) 1312
BsuRI GGCC 3 cut(s) 656, 802, 1380
BtgI CCRYGG 1 cut(s) 236
BtgZI GCGATG 1 cut(s) 426
BtrI CACGTC 1 cut(s) 85
BtsCI GGATG 1 cut(s) 1438
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 6 cut(s) 60, 543, 675, 1029, 1101, 1173
BveI ACCTGC 2 cut(s) 500, 599
Cac8I GCNNGC 2 cut(s) 259, 742
CciI TCATGA 1 cut(s) 531
CfoI GCGC 2 cut(s) 235, 1204
Cfr10I RCCGGY 1 cut(s) 377
Cfr13I GGNCC 4 cut(s) 203, 728, 773, 1286
CsiI ACCWGGT 1 cut(s) 1324
Csp6I GTAC 2 cut(s) 4, 858
CspCI CAANNNNNGTGG 2 cut(s) 1077, 1112
CviAII CATG 9 cut(s) 237, 523, 532, 575, 593, 798, 870, 1043, 1339
CviQI GTAC 2 cut(s) 4, 858
DdeI CTNAG 6 cut(s) 112, 187, 562, 790, 881, 1248
DpnI GATC 6 cut(s) 527, 690, 1140, 1314, 1473, 1505
DpnII GATC 6 cut(s) 525, 688, 1138, 1312, 1471, 1503
DraIII CACNNNGTG 1 cut(s) 104
EaeI YGGCCR 2 cut(s) 654, 1378
Eco130I CCWWGG 1 cut(s) 236
Eco47I GGWCC 4 cut(s) 203, 728, 773, 1286
Eco57I CTGAAG 1 cut(s) 1200
Eco91I GGTNACC 1 cut(s) 924
EcoO109I RGGNCCY 1 cut(s) 203
EcoO65I GGTNACC 1 cut(s) 924
EcoRII CCWGG 5 cut(s) 333, 735, 1069, 1236, 1324
EcoT14I CCWWGG 1 cut(s) 236
ErhI CCWWGG 1 cut(s) 236
FaeI CATG 9 cut(s) 240, 526, 535, 578, 596, 801, 873, 1046, 1342
FaqI GGGAC 1 cut(s) 759
FatI CATG 9 cut(s) 236, 522, 531, 574, 592, 797, 869, 1042, 1338
FauI CCCGC 1 cut(s) 709
FbaI TGATCA 2 cut(s) 688, 1471
Fnu4HI GCNGC 9 cut(s) 51, 184, 519, 606, 795, 894, 933, 1205, 1358
FokI GGATG 1 cut(s) 1445
Fsp4HI GCNGC 9 cut(s) 51, 184, 519, 606, 795, 894, 933, 1205, 1358
FspBI CTAG 2 cut(s) 1397, 1488
GlaI GCGC 2 cut(s) 234, 1203
GluI GCNGC 9 cut(s) 51, 184, 519, 606, 795, 894, 933, 1205, 1358
GsaI CCCAGC 1 cut(s) 1519
GsuI CTGGAG 1 cut(s) 996
HaeII RGCGCY 1 cut(s) 236
HaeIII GGCC 3 cut(s) 656, 802, 1380
HapII CCGG 3 cut(s) 378, 777, 1412
HhaI GCGC 2 cut(s) 235, 1204
Hin1II CATG 9 cut(s) 240, 526, 535, 578, 596, 801, 873, 1046, 1342
Hin6I GCGC 2 cut(s) 233, 1202
HinP1I GCGC 2 cut(s) 233, 1202
HinfI GANTC 7 cut(s) 135, 241, 323, 395, 827, 1188, 1348
HpaII CCGG 3 cut(s) 378, 777, 1412
HphI GGTGA 5 cut(s) 698, 869, 918, 1147, 1285
Hpy166II GTNNAC 4 cut(s) 82, 814, 973, 1368
Hpy188I TCNGA 8 cut(s) 19, 34, 165, 254, 980, 1143, 1180, 1471
Hpy188III TCNNGA 3 cut(s) 532, 723, 1256
Hpy8I GTNNAC 4 cut(s) 82, 814, 973, 1368
Hpy99I CGWCG 1 cut(s) 254
HpyAV CCTTC 5 cut(s) 160, 431, 1227, 1362, 1423
HpyCH4III ACNGT 4 cut(s) 250, 679, 1168, 1273
HpyCH4IV ACGT 1 cut(s) 84
HpyF10VI GCNNNNNNNGC 5 cut(s) 146, 155, 515, 741, 1034
HpyF3I CTNAG 6 cut(s) 112, 187, 562, 790, 881, 1248
HpySE526I ACGT 1 cut(s) 84
Hsp92II CATG 9 cut(s) 240, 526, 535, 578, 596, 801, 873, 1046, 1342
HspAI GCGC 2 cut(s) 233, 1202
Ksp22I TGATCA 2 cut(s) 688, 1471
Kzo9I GATC 6 cut(s) 525, 688, 1138, 1312, 1471, 1503
LmnI GCTCC 6 cut(s) 121, 155, 254, 703, 1015, 1421
Lsp1109I GCAGC 9 cut(s) 37, 170, 505, 592, 781, 905, 919, 1216, 1369
LweI GCATC 4 cut(s) 559, 627, 795, 1423
MabI ACCWGGT 1 cut(s) 1324
MaeI CTAG 2 cut(s) 1397, 1488
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 8 cut(s) 38, 360, 706, 924, 952, 1273, 1320, 1483
MalI GATC 6 cut(s) 527, 690, 1140, 1314, 1473, 1505
MboI GATC 6 cut(s) 525, 688, 1138, 1312, 1471, 1503
MboII GAAGA 5 cut(s) 489, 506, 551, 1427, 1498
MfeI CAATTG 1 cut(s) 948
MflI RGATCY 1 cut(s) 1312
MhlI GDGCHC 4 cut(s) 90, 746, 911, 1178
MlsI TGGCCA 2 cut(s) 656, 1380
MluNI TGGCCA 2 cut(s) 656, 1380
MlyI GAGTC 2 cut(s) 1197, 1357
MmeI TCCRAC 2 cut(s) 232, 1264
Mox20I TGGCCA 2 cut(s) 656, 1380
MscI TGGCCA 2 cut(s) 656, 1380
MseI TTAA 5 cut(s) 426, 843, 1047, 1317, 1362
MslI CAYNNNNRTG 3 cut(s) 882, 1343, 1499
Msp20I TGGCCA 2 cut(s) 656, 1380
MspA1I CMGCKG 3 cut(s) 191, 932, 962
MspCI CTTAAG 2 cut(s) 1316, 1361
MspI CCGG 3 cut(s) 378, 777, 1412
MspR9I CCNGG 7 cut(s) 335, 737, 777, 1071, 1238, 1326, 1412
MunI CAATTG 1 cut(s) 948
Mva1269I GAATGC 1 cut(s) 1477
MvaI CCWGG 5 cut(s) 335, 737, 1071, 1238, 1326
MwoI GCNNNNNNNGC 5 cut(s) 146, 155, 515, 741, 1034
NciI CCSGG 2 cut(s) 777, 1412
NcoI CCATGG 1 cut(s) 236
NdeII GATC 6 cut(s) 525, 688, 1138, 1312, 1471, 1503
NlaIII CATG 9 cut(s) 240, 526, 535, 578, 596, 801, 873, 1046, 1342
NlaIV GGNNCC 3 cut(s) 204, 421, 775
NmuCI GTSAC 5 cut(s) 38, 706, 924, 952, 1273
NspI RCATGY 2 cut(s) 596, 1046
OliI CACNNNNGTG 1 cut(s) 882
PagI TCATGA 1 cut(s) 531
PaqCI CACCTGC 1 cut(s) 500
PasI CCCWGGG 1 cut(s) 1070
PciI ACATGT 2 cut(s) 592, 1042
PctI GAATGC 1 cut(s) 1477
PfeI GAWTC 5 cut(s) 135, 241, 323, 395, 827
PfoI TCCNGGA 3 cut(s) 333, 1236, 1410
PkrI GCNGC 9 cut(s) 52, 185, 520, 607, 796, 895, 934, 1206, 1359
PleI GAGTC 2 cut(s) 1196, 1356
PpsI GAGTC 2 cut(s) 1196, 1356
PpuMI RGGWCCY 1 cut(s) 203
PscI ACATGT 2 cut(s) 592, 1042
Psp5II RGGWCCY 1 cut(s) 203
Psp6I CCWGG 5 cut(s) 333, 735, 1069, 1236, 1324
PspEI GGTNACC 1 cut(s) 924
PspFI CCCAGC 1 cut(s) 1515
PspGI CCWGG 5 cut(s) 333, 735, 1069, 1236, 1324
PspN4I GGNNCC 3 cut(s) 204, 421, 775
PspPI GGNCC 4 cut(s) 203, 728, 773, 1286
PspPPI RGGWCCY 1 cut(s) 203
PstI CTGCAG 1 cut(s) 610
PsuI RGATCY 1 cut(s) 1312
PvuII CAGCTG 2 cut(s) 191, 932
RsaI GTAC 2 cut(s) 5, 859
RsaNI GTAC 2 cut(s) 4, 858
RseI CAYNNNNRTG 3 cut(s) 882, 1343, 1499
SaqAI TTAA 5 cut(s) 426, 843, 1047, 1317, 1362
SatI GCNGC 9 cut(s) 51, 184, 519, 606, 795, 894, 933, 1205, 1358
Sau3AI GATC 6 cut(s) 525, 688, 1138, 1312, 1471, 1503
Sau96I GGNCC 4 cut(s) 203, 728, 773, 1286
SchI GAGTC 2 cut(s) 1197, 1357
ScrFI CCNGG 7 cut(s) 335, 737, 777, 1071, 1238, 1326, 1412
SduI GDGCHC 4 cut(s) 90, 746, 911, 1178
SexAI ACCWGGT 1 cut(s) 1324
SfaNI GCATC 4 cut(s) 559, 627, 795, 1423
SfcI CTRYAG 2 cut(s) 553, 606
SinI GGWCC 4 cut(s) 203, 728, 773, 1286
SmiMI CAYNNNNRTG 3 cut(s) 882, 1343, 1499
SmlI CTYRAG 4 cut(s) 902, 1254, 1316, 1361
SmoI CTYRAG 4 cut(s) 902, 1254, 1316, 1361
SsiI CCGC 3 cut(s) 702, 960, 1119
SspMI CTAG 2 cut(s) 1397, 1488
StyD4I CCNGG 7 cut(s) 333, 735, 775, 1069, 1236, 1324, 1410
StyI CCWWGG 1 cut(s) 236
TaaI ACNGT 4 cut(s) 250, 679, 1168, 1273
TaiI ACGT 1 cut(s) 87
TaqI TCGA 1 cut(s) 1509
TfiI GAWTC 5 cut(s) 135, 241, 323, 395, 827
Tru1I TTAA 5 cut(s) 426, 843, 1047, 1317, 1362
Tru9I TTAA 5 cut(s) 426, 843, 1047, 1317, 1362
TscAI CASTG 6 cut(s) 67, 550, 682, 1036, 1101, 1173
TseFI GTSAC 5 cut(s) 38, 706, 924, 952, 1273
TseI GCWGC 9 cut(s) 50, 183, 518, 605, 794, 893, 932, 1204, 1357
Tsp45I GTSAC 5 cut(s) 38, 706, 924, 952, 1273
TspDTI ATGAA 5 cut(s) 243, 548, 708, 1014, 1209
TspGWI ACGGA 1 cut(s) 147
TspRI CASTG 6 cut(s) 67, 550, 682, 1036, 1101, 1173
Vha464I CTTAAG 2 cut(s) 1316, 1361
VpaK11BI GGWCC 4 cut(s) 203, 728, 773, 1286
XapI RAATTY 4 cut(s) 171, 343, 355, 750
XceI RCATGY 2 cut(s) 596, 1046
XspI CTAG 2 cut(s) 1397, 1488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.