RchiOBHm_Chr7g0200181

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000302
Physical Location & Seq
Reverse (-)
18043036 .. 18045838
2803 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17915

Sequence Viewer

Length: 2295 bp
ATGGAGAACAAAAAGTGTGTAGTGATGCAGATCATATACCTTCTTGGCTTTAGTTGCATGTTTTACTTGTCACTAGCAGTTGCTGTCGAAAAAGTGGCAGTACTGGAACCACAAGGTGAGACAGATAGCATGCAAATTTATATAGTTTTGGTTGAAAGGCCACCCAGCAATTTCAATGCACAATCTGATCATGAAGATTTAGAGAGTTGGTACCAGTCATTTCTGCCCCAAACTATTGCAAACTCAAACCAGCTCATGAAGCCACGAATGGTTTATGCATACCACAATGTGGCCACAGGATTTGCAGCAAAGCTGACTGGGGAGGAAGTTGAAGAAATGGAGAAGAAAGATGGGTTTATCTACGCTCATCCAGAACGGAATCTGCAATTGCACACAACTCATAGTCCTAACTTCTTGGGGCTGCAACAAGGATTGGGACTTTGGAAAGGGTCTAATTATGGTGCAGGAATCATTATTGGAGTCTTGGATACTGGAATAACACCAGGTCATCCCTCATTCAGCGACGAAGGAGTGCCACCTCCTCCGGCTAAATGGAAAGGCAAGTGTGATTTCAATGGCACATTCTGCAACAACAAGCTTATTGGTGCCAGAAGTTTCCAGGGTGGGAACACTACCGGAGCTCCACCAGTTGACATTGCAGGCCATGGAACCCACACGTCGAGCACAGCTGGTGGAAATTTTGTGAAAGGTGCCAACGTGTTTGGAATGGCAAATGGAACAGCCTCTGGCATGGCACCTTATGCTCACTTGGCAATGTACCAAGTTTGTTCTGAACAGGGTTGTTCTGAAAGTGACATTCTAGCTGCTATTGACACTGCTATTGATGATGGCGTGGATGTGCTATCCATCTCACTTGGTGGTGGCTCAGCTACTTTTTATTGGGATTTTATTGCAATTGGTGCATTTGCAGCAATTCAGAAGGGAATTTTTGTCAGCTGTTCGGCTGGAAATGCAGGGCCTGACTACTCTACTACATCAAATGAGGCCCCATGGATTTTGACAGTTGGAGCAAGCTCCATTGACAGAAACATAAGAGCAATAGCACAGCTTGGAAATGGGCAAGAATATGACGGGGAATCAGTATTCCAGCCTAAAGATTTTAGTTCAAAACAGTTACCTCTTGTTTATGCAGGTGACCATAGCCATGATTCATCAGCTCTTTGCGATGTAGGGTCCCTTACAAATGTTGAAGGGAAAGTAGTGGTGTGTGAGCTAGGTGGCGGAATTGGAAGAATTGCAAAAGGGGTAGAAGTGAAAAGAGCTGGTGGTGTTGCCATGATTCTTGTCAACCCAGACTTTGCTGGCTATACCACCATAGCAGACGTTCACGTGCTTCCGGCAACACATGTAAGTTACTCTGCGGGGGTGAGTATCAAAACCTATATAAACTCAACCTCAACTCCTACAGCAACAATCTTGTTCAAAGGCACTGTCATTGGTGATCAGCTTGCTCCGAAGGTCTCTTTCTTTTCATCAAGAGGACCAAGCCTCGCAAGTCCTGGCATTTTGAAACCTGACATAATTGGTCCCGGTGTGAGCATCCTAGCTGCATGGCCTTTTTCGGTGGACAATGCCACAGAATCTAAAGCCACATTTAACATTATTTCCGGTACTTCAATGTCATGCCCTCACCTGAGTGGCATTGTAGCCTTACTCAAGAGCTCACACCCTGACTGGTCACCAGCTGCCATTAAGTCTGCCATCATGACAACTGCTGAAGTAAACAACCTCGCAGGCTCCGCCATAATGGATGAAACACTATTTGCAGCAGACCTCTTTGCCATTGGTGCAGGTCATGTTAACCCTTCAAAAGCAAATGACCCTGGGCTCATCTACGACACGCAACCAGATGACTACATTCCTTACATGTGTGGTTTGAACTACACAGACCAACAGATAGCAATAGTCACCCGGAAATTAGTGAAATGCTCTGAAGTAGGAGCCATACCCGAAGCACAGCTAAATTATCCCACATTTTCTCTCTTTATAGATCCTGCAGGGAAGCCTCAGCATTACACAAGAACAGTGAAGAATGTTGGCCCCGCTAAATCAACTTATGAATTAGCCGTTGTAAGTCCACATAACATAGACATCAGTGTGCAACCTGAGGAGCTCACATTCACAGAGGTTAACCAGACAATGACGTATCATGTGGTGTTTACCGCACAAAAAGGTGCTGCGAAGAATGGTATAAAATTTTCTCAGGGATATCTGAGCTGGGTCTCTCACCAGCATTCTGTTAGAAGCCAAATATCTGTGATCTTTGACACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

764

Amino Acids

81.19

Weight (kDa)

5.42

Isoelectric Point (pI)

33.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 59 - 131 9.2e-11 Peptidase inhibitor I9
Peptidase_S8 PF00082 154 - 582 1.4e-45 Subtilase family
PA PF02225 378 - 464 8.6e-11 PA domain
fn3_6 PF17766 660 - 760 1.4e-25 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1142
Acc36I ACCTGC 2 cut(s) 1142, 1802
Acc65I GGTACC 1 cut(s) 210
AccB1I GGYRCC 4 cut(s) 210, 605, 710, 754
AccB7I CCANNNNNTGG 1 cut(s) 289
AciI CCGC 5 cut(s) 1242, 1382, 1761, 2064, 2184
AclWI GGATC 1 cut(s) 2006
AcoI YGGCCR 1 cut(s) 291
AcsI RAATTY 4 cut(s) 135, 697, 945, 2216
AcuI CTGAAG 2 cut(s) 1758, 1974
AcvI CACGTG 1 cut(s) 1351
AdeI CACNNNGTG 3 cut(s) 116, 289, 878
AfaI GTAC 4 cut(s) 102, 212, 779, 1633
AfiI CCNNNNNNNGG 1 cut(s) 289
AflIII ACRYGT 4 cut(s) 675, 717, 1366, 1887
AjiI CACGTC 1 cut(s) 678
AjnI CCWGG 4 cut(s) 502, 618, 1519, 1843
AleI CACNNNNGTG 1 cut(s) 1656
Alw21I GWGCWC 4 cut(s) 643, 686, 1685, 2136
Alw26I GTCTC 3 cut(s) 113, 1486, 2248
AlwI GGATC 1 cut(s) 2006
AlwNI CAGNNNCTG 3 cut(s) 83, 746, 980
AoxI GGCC 7 cut(s) 158, 291, 661, 977, 1005, 1574, 2059
ApeKI GCWGC 8 cut(s) 305, 421, 824, 929, 1568, 1706, 1787, 2198
ApoI RAATTY 4 cut(s) 135, 697, 945, 2216
ArsI GACNNNNNNTTYG 2 cut(s) 1437, 1469
Asp718I GGTACC 1 cut(s) 210
AspS9I GGNCC 6 cut(s) 977, 1006, 1194, 1502, 1547, 2060
AsuC2I CCSGG 2 cut(s) 1551, 1933
AsuHPI GGTGA 8 cut(s) 128, 1166, 1399, 1472, 1643, 1692, 1921, 2240
AvaII GGWCC 3 cut(s) 1194, 1502, 1547
AxyI CCTNAGG 1 cut(s) 2127
BalI TGGCCA 1 cut(s) 293
BanI GGYRCC 4 cut(s) 210, 605, 710, 754
BanII GRGCYC 4 cut(s) 643, 1685, 1851, 2136
BbrPI CACGTG 1 cut(s) 1351
Bbv12I GWGCWC 4 cut(s) 643, 686, 1685, 2136
BbvCI CCTCAGC 1 cut(s) 2028
BbvI GCAGC 8 cut(s) 317, 408, 811, 941, 1555, 1693, 1799, 2185
BccI CCATC 4 cut(s) 344, 842, 875, 1730
BceAI ACGGC 1 cut(s) 2072
BciT130I CCWGG 4 cut(s) 504, 620, 1521, 1845
BciVI GTATCC 1 cut(s) 481
BclI TGATCA 2 cut(s) 187, 1462
BcnI CCSGG 2 cut(s) 1551, 1933
BcoDI GTCTC 3 cut(s) 113, 1486, 2248
BfaI CTAG 4 cut(s) 74, 821, 1235, 1565
BfmI CTRYAG 2 cut(s) 1425, 2016
BfuAI ACCTGC 2 cut(s) 1142, 1802
BfuI GTATCC 1 cut(s) 481
BisI GCNGC 8 cut(s) 306, 422, 825, 930, 1569, 1707, 1788, 2199
BlpI GCTNAGC 1 cut(s) 886
BlsI GCNGC 8 cut(s) 307, 423, 826, 931, 1570, 1708, 1789, 2200
BmcAI AGTACT 1 cut(s) 102
Bme1390I CCNGG 6 cut(s) 504, 620, 1521, 1551, 1845, 1933
Bme18I GGWCC 3 cut(s) 1194, 1502, 1547
BmgBI CACGTC 1 cut(s) 678
BmgT120I GGNCC 6 cut(s) 977, 1006, 1194, 1502, 1547, 2060
BmrFI CCNGG 6 cut(s) 504, 620, 1521, 1551, 1845, 1933
BmrI ACTGGG 1 cut(s) 327
BmsI GCATC 2 cut(s) 15, 1569
BmuI ACTGGG 1 cut(s) 327
Bpu10I CCTNAGC 1 cut(s) 2028
Bpu1102I GCTNAGC 1 cut(s) 886
BpuEI CTTGAG 1 cut(s) 1661
BpuMI CCSGG 2 cut(s) 1551, 1933
BsaAI YACGTR 1 cut(s) 1351
BsaBI GATNNNNATC 1 cut(s) 29
BsaI GGTCTC 2 cut(s) 1486, 2248
BsaJI CCNNGG 5 cut(s) 619, 664, 1010, 1843, 1844
BsaWI WCCGGW 2 cut(s) 635, 1628
BsaXI ACNNNNNCTCC 4 cut(s) 625, 655, 1405, 1435
Bsc4I CCNNNNNNNGG 1 cut(s) 289
Bse1I ACTGG 6 cut(s) 108, 214, 322, 496, 647, 1700
Bse21I CCTNAGG 1 cut(s) 2127
Bse3DI GCAATG 2 cut(s) 654, 780
Bse8I GATNNNNATC 1 cut(s) 29
BseBI CCWGG 4 cut(s) 504, 620, 1521, 1845
BseDI CCNNGG 5 cut(s) 619, 664, 1010, 1843, 1844
BseGI GGATG 5 cut(s) 367, 508, 862, 1560, 1777
BseJI GATNNNNATC 1 cut(s) 29
BseLI CCNNNNNNNGG 1 cut(s) 289
BseMI GCAATG 2 cut(s) 654, 780
BseMII CTCAG 6 cut(s) 900, 1646, 2042, 2118, 2225, 2237
BseNI ACTGG 6 cut(s) 108, 214, 322, 496, 647, 1700
BseRI GAGGAG 2 cut(s) 531, 2144
BseXI GCAGC 8 cut(s) 317, 408, 811, 941, 1555, 1693, 1799, 2185
BseYI CCCAGC 2 cut(s) 164, 2238
BsgI GTGCAG 2 cut(s) 483, 1830
BshFI GGCC 7 cut(s) 160, 293, 663, 979, 1007, 1576, 2061
BshNI GGYRCC 4 cut(s) 210, 605, 710, 754
BsiHKAI GWGCWC 4 cut(s) 643, 686, 1685, 2136
BsiSI CCGG 6 cut(s) 545, 636, 1358, 1551, 1629, 1933
BslFI GGGAC 3 cut(s) 450, 1180, 1533
BslI CCNNNNNNNGG 1 cut(s) 289
BsmAI GTCTC 3 cut(s) 113, 1486, 2248
BsmFI GGGAC 3 cut(s) 450, 1180, 1533
BsmI GAATGC 1 cut(s) 2254
BsnI GGCC 7 cut(s) 160, 293, 663, 979, 1007, 1576, 2061
Bso31I GGTCTC 2 cut(s) 1486, 2248
Bsp1286I GDGCHC 5 cut(s) 643, 686, 1685, 1851, 2136
Bsp143I GATC 5 cut(s) 30, 187, 1462, 2011, 2280
Bsp1720I GCTNAGC 1 cut(s) 886
Bsp19I CCATGG 2 cut(s) 664, 1010
BspACI CCGC 5 cut(s) 1242, 1382, 1761, 2064, 2184
BspANI GGCC 7 cut(s) 160, 293, 663, 979, 1007, 1576, 2061
BspCNI CTCAG 6 cut(s) 899, 1647, 2041, 2119, 2226, 2236
BspHI TCATGA 3 cut(s) 190, 255, 1725
BspMAI CTGCAG 1 cut(s) 2020
BspMI ACCTGC 2 cut(s) 1142, 1802
BspPI GGATC 1 cut(s) 2006
BspT107I GGYRCC 4 cut(s) 210, 605, 710, 754
BspTNI GGTCTC 2 cut(s) 1486, 2248
BsrDI GCAATG 2 cut(s) 654, 780
BsrI ACTGG 6 cut(s) 108, 214, 322, 496, 647, 1700
BssECI CCNNGG 5 cut(s) 619, 664, 1010, 1843, 1844
BssMI GATC 5 cut(s) 30, 187, 1462, 2011, 2280
BssT1I CCWWGG 2 cut(s) 664, 1010
Bst2UI CCWGG 4 cut(s) 504, 620, 1521, 1845
Bst4CI ACNGT 4 cut(s) 1024, 1134, 1453, 2047
BstAPI GCANNNNNTGC 3 cut(s) 585, 761, 920
BstBAI YACGTR 1 cut(s) 1351
BstC8I GCNNGC 6 cut(s) 131, 661, 1033, 1324, 1470, 1756
BstDEI CTNAG 6 cut(s) 886, 1655, 2028, 2127, 2223, 2234
BstDSI CCRYGG 2 cut(s) 664, 1010
BstEII GGTNACC 2 cut(s) 1154, 1698
BstF5I GGATG 5 cut(s) 367, 508, 862, 1560, 1777
BstKTI GATC 5 cut(s) 33, 190, 1465, 2014, 2283
BstMAI GTCTC 3 cut(s) 113, 1486, 2248
BstMBI GATC 5 cut(s) 30, 187, 1462, 2011, 2280
BstNI CCWGG 4 cut(s) 504, 620, 1521, 1845
BstNSI RCATGY 4 cut(s) 61, 133, 1370, 1891
BstPI GGTNACC 2 cut(s) 1154, 1698
BstSCI CCNGG 6 cut(s) 502, 618, 1519, 1549, 1843, 1931
BstSFI CTRYAG 2 cut(s) 1425, 2016
BstV1I GCAGC 8 cut(s) 317, 408, 811, 941, 1555, 1693, 1799, 2185
BstX2I RGATCY 1 cut(s) 2011
BstYI RGATCY 1 cut(s) 2011
Bsu36I CCTNAGG 1 cut(s) 2127
BsuI GTATCC 1 cut(s) 481
BsuRI GGCC 7 cut(s) 160, 293, 663, 979, 1007, 1576, 2061
BtgI CCRYGG 2 cut(s) 664, 1010
BtgZI GCGATG 1 cut(s) 1200
BtrI CACGTC 1 cut(s) 678
BtsCI GGATG 5 cut(s) 367, 508, 862, 1560, 1777
BtsI GCAGTG 1 cut(s) 834
BtsIMutI CAGTG 4 cut(s) 834, 1449, 2052, 2122
BveI ACCTGC 2 cut(s) 1142, 1802
Cac8I GCNNGC 6 cut(s) 131, 661, 1033, 1324, 1470, 1756
CaiI CAGNNNCTG 3 cut(s) 83, 746, 980
CciI TCATGA 3 cut(s) 190, 255, 1725
Cfr13I GGNCC 6 cut(s) 977, 1006, 1194, 1502, 1547, 2060
CsiI ACCWGGT 1 cut(s) 502
Csp6I GTAC 4 cut(s) 101, 211, 778, 1632
CspCI CAANNNNNGTGG 2 cut(s) 283, 318
CviQI GTAC 4 cut(s) 101, 211, 778, 1632
DdeI CTNAG 6 cut(s) 886, 1655, 2028, 2127, 2223, 2234
DpnI GATC 5 cut(s) 32, 189, 1464, 2013, 2282
DpnII GATC 5 cut(s) 30, 187, 1462, 2011, 2280
DraIII CACNNNGTG 3 cut(s) 116, 289, 878
EaeI YGGCCR 1 cut(s) 291
EciI GGCGGA 2 cut(s) 1257, 1750
Ecl136II GAGCTC 3 cut(s) 641, 1683, 2134
Eco130I CCWWGG 2 cut(s) 664, 1010
Eco24I GRGCYC 4 cut(s) 643, 1685, 1851, 2136
Eco31I GGTCTC 2 cut(s) 1486, 2248
Eco32I GATATC 1 cut(s) 2231
Eco47I GGWCC 3 cut(s) 1194, 1502, 1547
Eco53kI GAGCTC 3 cut(s) 641, 1683, 2134
Eco57I CTGAAG 2 cut(s) 1758, 1974
Eco72I CACGTG 1 cut(s) 1351
Eco81I CCTNAGG 1 cut(s) 2127
Eco91I GGTNACC 2 cut(s) 1154, 1698
EcoICRI GAGCTC 3 cut(s) 641, 1683, 2134
EcoO109I RGGNCCY 3 cut(s) 977, 1006, 1194
EcoO65I GGTNACC 2 cut(s) 1154, 1698
EcoRII CCWGG 4 cut(s) 502, 618, 1519, 1843
EcoRV GATATC 1 cut(s) 2231
EcoT14I CCWWGG 2 cut(s) 664, 1010
EcoT22I ATGCAT 1 cut(s) 280
EcoT38I GRGCYC 4 cut(s) 643, 1685, 1851, 2136
ErhI CCWWGG 2 cut(s) 664, 1010
FaqI GGGAC 3 cut(s) 450, 1180, 1533
FauI CCCGC 2 cut(s) 1375, 2071
FbaI TGATCA 2 cut(s) 187, 1462
Fnu4HI GCNGC 8 cut(s) 306, 422, 825, 930, 1569, 1707, 1788, 2199
FokI GGATG 5 cut(s) 354, 495, 869, 1547, 1784
FriOI GRGCYC 4 cut(s) 643, 1685, 1851, 2136
Fsp4HI GCNGC 8 cut(s) 306, 422, 825, 930, 1569, 1707, 1788, 2199
FspBI CTAG 4 cut(s) 74, 821, 1235, 1565
GluI GCNGC 8 cut(s) 306, 422, 825, 930, 1569, 1707, 1788, 2199
GsaI CCCAGC 2 cut(s) 168, 2242
HaeIII GGCC 7 cut(s) 160, 293, 663, 979, 1007, 1576, 2061
HapII CCGG 6 cut(s) 545, 636, 1358, 1551, 1629, 1933
HincII GTYRAC 4 cut(s) 652, 1309, 1822, 2152
HindII GTYRAC 4 cut(s) 652, 1309, 1822, 2152
HindIII AAGCTT 1 cut(s) 596
HinfI GANTC 7 cut(s) 379, 468, 480, 1097, 1169, 1300, 1601
HpaI GTTAAC 2 cut(s) 1822, 2152
HpaII CCGG 6 cut(s) 545, 636, 1358, 1551, 1629, 1933
HphI GGTGA 8 cut(s) 128, 1166, 1399, 1472, 1643, 1692, 1921, 2240
Hpy166II GTNNAC 9 cut(s) 652, 1309, 1348, 1588, 1744, 1822, 2099, 2152, 2181
Hpy188I TCNGA 7 cut(s) 187, 793, 808, 939, 1476, 1954, 2235
Hpy188III TCNNGA 6 cut(s) 191, 256, 371, 1497, 1678, 1726
Hpy8I GTNNAC 9 cut(s) 652, 1309, 1348, 1588, 1744, 1822, 2099, 2152, 2181
Hpy99I CGWCG 2 cut(s) 527, 682
HpyAV CCTTC 6 cut(s) 50, 521, 934, 1205, 1471, 1836
HpyCH4III ACNGT 4 cut(s) 1024, 1134, 1453, 2047
HpyCH4IV ACGT 5 cut(s) 677, 717, 1344, 1350, 2165
HpyF3I CTNAG 6 cut(s) 886, 1655, 2028, 2127, 2223, 2234
HpySE526I ACGT 5 cut(s) 677, 717, 1344, 1350, 2165
KflI GGGWCCC 1 cut(s) 1194
KpnI GGTACC 1 cut(s) 214
Ksp22I TGATCA 2 cut(s) 187, 1462
KspAI GTTAAC 2 cut(s) 1822, 2152
Kzo9I GATC 5 cut(s) 30, 187, 1462, 2011, 2280
LmnI GCTCC 8 cut(s) 638, 646, 1028, 1040, 1477, 1763, 1961, 2131
Lsp1109I GCAGC 8 cut(s) 317, 408, 811, 941, 1555, 1693, 1799, 2185
LweI GCATC 2 cut(s) 15, 1569
MabI ACCWGGT 1 cut(s) 502
MaeI CTAG 4 cut(s) 74, 821, 1235, 1565
MaeII ACGT 5 cut(s) 677, 717, 1344, 1350, 2165
MaeIII GTNAC 7 cut(s) 69, 812, 1134, 1154, 1373, 1698, 1927
MalI GATC 5 cut(s) 32, 189, 1464, 2013, 2282
MboI GATC 5 cut(s) 30, 187, 1462, 2011, 2280
MboII GAAGA 6 cut(s) 206, 344, 355, 1263, 2062, 2215
MfeI CAATTG 2 cut(s) 386, 915
MflI RGATCY 1 cut(s) 2011
MhlI GDGCHC 5 cut(s) 643, 686, 1685, 1851, 2136
MlsI TGGCCA 1 cut(s) 293
MluNI TGGCCA 1 cut(s) 293
MlyI GAGTC 1 cut(s) 489
MmeI TCCRAC 1 cut(s) 1006
Mox20I TGGCCA 1 cut(s) 293
Mph1103I ATGCAT 1 cut(s) 280
MscI TGGCCA 1 cut(s) 293
MseI TTAA 5 cut(s) 1617, 1713, 1821, 2151, 2293
MslI CAYNNNNRTG 3 cut(s) 1164, 1656, 2117
Msp20I TGGCCA 1 cut(s) 293
MspA1I CMGCKG 3 cut(s) 689, 957, 1706
MspI CCGG 6 cut(s) 545, 636, 1358, 1551, 1629, 1933
MspR9I CCNGG 6 cut(s) 504, 620, 1521, 1551, 1845, 1933
MunI CAATTG 2 cut(s) 386, 915
Mva1269I GAATGC 1 cut(s) 2254
MvaI CCWGG 4 cut(s) 504, 620, 1521, 1845
NciI CCSGG 2 cut(s) 1551, 1933
NcoI CCATGG 2 cut(s) 664, 1010
NdeII GATC 5 cut(s) 30, 187, 1462, 2011, 2280
NmuCI GTSAC 5 cut(s) 69, 812, 1154, 1698, 1927
NsiI ATGCAT 1 cut(s) 280
NspI RCATGY 4 cut(s) 61, 133, 1370, 1891
OliI CACNNNNGTG 1 cut(s) 1656
PaeI GCATGC 1 cut(s) 133
PagI TCATGA 3 cut(s) 190, 255, 1725
PaqCI CACCTGC 1 cut(s) 1142
PasI CCCWGGG 1 cut(s) 1844
PciI ACATGT 2 cut(s) 1366, 1887
PctI GAATGC 1 cut(s) 2254
PfeI GAWTC 6 cut(s) 379, 468, 1097, 1169, 1300, 1601
PflMI CCANNNNNTGG 1 cut(s) 289
PkrI GCNGC 8 cut(s) 307, 423, 826, 931, 1570, 1708, 1789, 2200
PleI GAGTC 1 cut(s) 488
PmaCI CACGTG 1 cut(s) 1351
PmlI CACGTG 1 cut(s) 1351
PpsI GAGTC 1 cut(s) 488
Ppu21I YACGTR 1 cut(s) 1351
PpuMI RGGWCCY 1 cut(s) 1194
PscI ACATGT 2 cut(s) 1366, 1887
Psp124BI GAGCTC 3 cut(s) 643, 1685, 2136
Psp5II RGGWCCY 1 cut(s) 1194
Psp6I CCWGG 4 cut(s) 502, 618, 1519, 1843
PspCI CACGTG 1 cut(s) 1351
PspEI GGTNACC 2 cut(s) 1154, 1698
PspFI CCCAGC 2 cut(s) 164, 2238
PspGI CCWGG 4 cut(s) 502, 618, 1519, 1843
PspPI GGNCC 6 cut(s) 977, 1006, 1194, 1502, 1547, 2060
PspPPI RGGWCCY 1 cut(s) 1194
PstI CTGCAG 1 cut(s) 2020
PstNI CAGNNNCTG 3 cut(s) 83, 746, 980
PsuI RGATCY 1 cut(s) 2011
PvuII CAGCTG 3 cut(s) 689, 957, 1706
RsaI GTAC 4 cut(s) 102, 212, 779, 1633
RsaNI GTAC 4 cut(s) 101, 211, 778, 1632
RseI CAYNNNNRTG 3 cut(s) 1164, 1656, 2117
SacI GAGCTC 3 cut(s) 643, 1685, 2136
SaqAI TTAA 5 cut(s) 1617, 1713, 1821, 2151, 2293
SatI GCNGC 8 cut(s) 306, 422, 825, 930, 1569, 1707, 1788, 2199
Sau3AI GATC 5 cut(s) 30, 187, 1462, 2011, 2280
Sau96I GGNCC 6 cut(s) 977, 1006, 1194, 1502, 1547, 2060
SbfI CCTGCAGG 1 cut(s) 2020
ScaI AGTACT 1 cut(s) 102
SchI GAGTC 1 cut(s) 489
ScrFI CCNGG 6 cut(s) 504, 620, 1521, 1551, 1845, 1933
SdaI CCTGCAGG 1 cut(s) 2020
SduI GDGCHC 5 cut(s) 643, 686, 1685, 1851, 2136
SexAI ACCWGGT 1 cut(s) 502
SfaNI GCATC 2 cut(s) 15, 1569
SfcI CTRYAG 2 cut(s) 1425, 2016
SinI GGWCC 3 cut(s) 1194, 1502, 1547
SmiMI CAYNNNNRTG 3 cut(s) 1164, 1656, 2117
SmlI CTYRAG 1 cut(s) 1676
SmoI CTYRAG 1 cut(s) 1676
SphI GCATGC 1 cut(s) 133
Sse8387I CCTGCAGG 1 cut(s) 2020
SsiI CCGC 5 cut(s) 1242, 1382, 1761, 2064, 2184
SspMI CTAG 4 cut(s) 74, 821, 1235, 1565
SstI GAGCTC 3 cut(s) 643, 1685, 2136
StyD4I CCNGG 6 cut(s) 502, 618, 1519, 1549, 1843, 1931
StyI CCWWGG 2 cut(s) 664, 1010
TaaI ACNGT 4 cut(s) 1024, 1134, 1453, 2047
TaiI ACGT 5 cut(s) 680, 720, 1347, 1353, 2168
TaqI TCGA 2 cut(s) 87, 680
TatI WGTACW 1 cut(s) 100
TfiI GAWTC 6 cut(s) 379, 468, 1097, 1169, 1300, 1601
Tru1I TTAA 5 cut(s) 1617, 1713, 1821, 2151, 2293
Tru9I TTAA 5 cut(s) 1617, 1713, 1821, 2151, 2293
TscAI CASTG 4 cut(s) 841, 1456, 2052, 2122
TseFI GTSAC 5 cut(s) 69, 812, 1154, 1698, 1927
TseI GCWGC 8 cut(s) 305, 421, 824, 929, 1568, 1706, 1787, 2198
Tsp45I GTSAC 5 cut(s) 69, 812, 1154, 1698, 1927
TspDTI ATGAA 6 cut(s) 207, 272, 1161, 1482, 1788, 2094
TspGWI ACGGA 1 cut(s) 391
TspRI CASTG 4 cut(s) 841, 1456, 2052, 2122
Van91I CCANNNNNTGG 1 cut(s) 289
VpaK11BI GGWCC 3 cut(s) 1194, 1502, 1547
XapI RAATTY 4 cut(s) 135, 697, 945, 2216
XceI RCATGY 4 cut(s) 61, 133, 1370, 1891
XspI CTAG 4 cut(s) 74, 821, 1235, 1565
ZrmI AGTACT 1 cut(s) 102
Zsp2I ATGCAT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.