Rw1G014970

Subtilisin-like serine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000302
Physical Location & Seq
Reverse (-)
33311950 .. 33314431
2482 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G014970.1

Sequence Viewer

Length: 2085 bp
ATGGAGAATAAAAGGAGTGTAGCGATTCGATTTACCTATCTTCTTGGCTTCATTTTCATGTTTCATCTATCACTAGCAGTACTTGCTGATGAAAATGATAGTCCCGCAGACGAAGTTCCTGCACTATATTCTCAAGATTTAGAGAGTTGGTATCAGACATTGCTGCCGGAAAGTACTATTGCAAGCTCAAATAAGCCCAGCATTCCGCGAATGGTTCACTCATACAGCAATGTGGCCACTGGCTTTGCAGCGAAACTGACACCAGAGGAAGTGAAAGCAATGGAAAAGAAAGATGGGTTCGTGCATGCTCATCCAGAGAAGTTTCTGCCTTTGCACACTAGTCGTAGTGTGATCGTAGGAGTTTTGGACACTGGGATATCTCCAGATCATCCCTCATTTAGTGATGAAGGAGTGCCACCACCTCCGGTGAAATGGAAAGGCAAATGTGAGTTCAAGTTCAATGGTACGACAGTGTGCAACAACAAGCTTATTGGTGCAAGAAATTTCCAAGGTGGGAAAGAAACAAAAGAAGTCTCTCCATTTGGTGAAGAAGGCCATGACACTCTCACTTCTAGCATAGCTGCTGGAAACTTTGTGAAAGGCGCCAGCGTGTTTGGAATGGCAAATGGCACAGCAGCTGGCATTGCACCTTATGCTCACTTGGCCATCTACAAGGTCTGCACCGAGGCAGGTTGTGCCGAAAGTGATATGCTAGCAGCTCTTGACACTGCTGTTGAGGACAGTGTGGATGTGCTCTCACTGTCCATAGGTTTTGCTTCCTCAGTTCCTTTCCATCAAGATGGGGTTGCTATCGGTGCGTTTGGAGCAATTCGAAAGGGAATTTTTGTCAGCTGTTCAGCTGGAAACTCTGGCCCTTTCTATAGTTCCATGTCAAACAAGGCCCCTTGGATTCTCACCGTTGGAGCAAGTACCATTGACAGAAAAATAAGCTCAACAGCACAGCTTGGAGATTCTCCAAAATATTTTTACCCTGTTGAATCTTTATTCAATCCTAAAAATTTTAGCACTTCACCATTGTTACCTCTTGTTTATCCAGGTGCAAATCCCAATAACCAAACTGAGTTCTGTGCCTTGGGATCTCTTGTAAATGTTGAAGGGAAAGTAGTGGTGTGTGAAAGAGGTGGAGGAGTGGGAAGAACTGCCAAAGGGGAAGAAGTGAAAAAAGCAGGAGGTGCTGCCATGATTCTAGTGAACCAAATCACTAATGACTATAGCACCTTAGCTGATGCTCATGTACTTCCAGCAACAAATGTTGGTTTTCAAGCAGGTTTGAGTATCAAAACCTATATAAAATCAACAACAACACCTATAGCCACAGTCTATTCTCCACGCACTGAAATAGGAGATACACTTGCTCCAATGGTTGCTTCTTTCTCATCAAGAGGACCGAGCATAGCTAGCCCCGGAATTTTGAAACCTGATATCATTGGTCCTGGTCTTAATATCCTCGCTGCATGGCCTGTTTCGGTGGATAATGTCACAACATTTGACATTCTTTCAGGTACCTCAGTGTCAAGCCCACACCTAAGTGGCATTGCAGCCTTGATCAAGAGTTCACACCCGGACCGGTCACCTGCTGCCATTAAATCGGCCATTATGACAACCGCTGATATGCTAAATCTCGAAGGCACACCAATTGTTGATAGATCAAATATCCCAGCAGACATCTACAGCACTGGCTCAGGCCATATGATCACCCAAGAAAGAGTGGACTGCTCTGAGGTAGGAGTCACAGCTGAAGCGCAGCTAAACTATCCTTCATTTTCTATCACAATAGGGTCCTATCAAACTCAGAATTATACAAGAACTGTGACAAATGTTGGCCCGGCTTATTCAACCTACAAATCAGAGATTTTGAAACCCGAGAAATGGGGTGTCAGTGTGAACATGATCGTGACTCCTTCGGTGCTTACATTCACAGAGGTCAACCAGACCATTACAAACAATGTGGAGTTCTCCACACAACTAGCTGGGCAAGATGGTTCATTTTCACAGGGATATTTGAGATGGGTTTCTGACAAGTATTCTGTATACAGCCCGATAGCTGTCATCTTTCACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

694

Amino Acids

73.72

Weight (kDa)

5.66

Isoelectric Point (pI)

34.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 43 - 112 8.7e-09 Peptidase inhibitor I9
Peptidase_S8 PF00082 115 - 546 4.4e-39 Subtilase family
PA PF02225 348 - 432 1.6e-11 PA domain
fn3_6 PF17766 589 - 690 1.3e-21 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1603
Acc36I ACCTGC 3 cut(s) 680, 1277, 1603
Acc65I GGTACC 1 cut(s) 1523
AccB1I GGYRCC 2 cut(s) 602, 1523
AccI GTMKAC 1 cut(s) 2052
AccII CGCG 1 cut(s) 208
AciI CCGC 3 cut(s) 105, 206, 1626
AclWI GGATC 1 cut(s) 1105
AcoI YGGCCR 3 cut(s) 234, 663, 1611
AcsI RAATTY 4 cut(s) 502, 840, 1018, 1428
AcuI CTGAAG 1 cut(s) 1779
AcyI GRCGYC 1 cut(s) 603
AfaI GTAC 6 cut(s) 81, 175, 466, 931, 1257, 1525
AfiI CCNNNNNNNGG 1 cut(s) 1890
AgeI ACCGGT 1 cut(s) 1587
AgsI TTSAA 9 cut(s) 454, 460, 998, 1009, 1115, 1283, 1435, 1857, 1879
AhlI ACTAGT 1 cut(s) 338
AjnI CCWGG 2 cut(s) 1054, 1453
AjuI GAANNNNNNNTTGG 2 cut(s) 1372, 1404
AleI CACNNNNGTG 1 cut(s) 1548
Alw21I GWGCWC 1 cut(s) 756
Alw26I GTCTC 1 cut(s) 538
AlwI GGATC 1 cut(s) 1105
AlwNI CAGNNNCTG 1 cut(s) 638
Ama87I CYCGRG 1 cut(s) 1883
AoxI GGCC 9 cut(s) 234, 553, 663, 871, 900, 1478, 1611, 1705, 1843
ApoI RAATTY 4 cut(s) 502, 840, 1018, 1428
AsiGI ACCGGT 1 cut(s) 1587
Asp718I GGTACC 1 cut(s) 1523
AspLEI GCGC 2 cut(s) 605, 1765
AspS9I GGNCC 7 cut(s) 872, 901, 1406, 1451, 1585, 1800, 1844
AsuC2I CCSGG 3 cut(s) 1425, 1583, 1847
AsuHPI GGTGA 6 cut(s) 439, 557, 907, 1023, 1584, 1708
AsuII TTCGAA 1 cut(s) 832
AsuNHI GCTAGC 2 cut(s) 712, 1418
AvaI CYCGRG 1 cut(s) 1883
AvaII GGWCC 4 cut(s) 1406, 1451, 1585, 1800
BaeI ACNNNNGTAYC 4 cut(s) 456, 489, 1515, 1548
BalI TGGCCA 2 cut(s) 236, 665
BanI GGYRCC 2 cut(s) 602, 1523
Bbv12I GWGCWC 1 cut(s) 756
BccI CCATC 6 cut(s) 287, 674, 794, 801, 1994, 2022
BcgI CGANNNNNNTGC 4 cut(s) 101, 135, 323, 357
BciT130I CCWGG 2 cut(s) 1056, 1455
BclI TGATCA 2 cut(s) 1566, 1713
BcnI CCSGG 3 cut(s) 1425, 1583, 1847
BcoDI GTCTC 1 cut(s) 538
BcuI ACTAGT 1 cut(s) 338
BfaI CTAG 7 cut(s) 74, 339, 573, 713, 1208, 1419, 1988
BfmI CTRYAG 4 cut(s) 880, 1231, 1329, 1690
BfoI RGCGCY 1 cut(s) 606
BfuAI ACCTGC 3 cut(s) 680, 1277, 1603
BmcAI AGTACT 2 cut(s) 81, 175
Bme1390I CCNGG 5 cut(s) 1056, 1425, 1455, 1583, 1847
Bme18I GGWCC 4 cut(s) 1406, 1451, 1585, 1800
BmeT110I CYCGRG 1 cut(s) 1883
BmgT120I GGNCC 7 cut(s) 872, 901, 1406, 1451, 1585, 1800, 1844
BmiI GGNNCC 4 cut(s) 604, 903, 1525, 1801
BmrFI CCNGG 5 cut(s) 1056, 1425, 1455, 1583, 1847
BmrI ACTGGG 1 cut(s) 381
BmsI GCATC 1 cut(s) 1237
BmtI GCTAGC 2 cut(s) 716, 1422
BmuI ACTGGG 1 cut(s) 381
BpmI CTGGAG 1 cut(s) 366
Bpu10I CCTNAGC 2 cut(s) 1240, 1702
Bpu14I TTCGAA 1 cut(s) 832
BpuEI CTTGAG 1 cut(s) 117
BpuMI CCSGG 3 cut(s) 1425, 1583, 1847
BsaHI GRCGYC 1 cut(s) 603
BsaJI CCNNGG 5 cut(s) 508, 684, 905, 1092, 1423
BsaWI WCCGGW 2 cut(s) 424, 1587
BsaXI ACNNNNNCTCC 2 cut(s) 1360, 1390
Bsc4I CCNNNNNNNGG 1 cut(s) 1890
Bse118I RCCGGY 1 cut(s) 1587
Bse1I ACTGG 3 cut(s) 244, 376, 1702
Bse3DI GCAATG 5 cut(s) 158, 235, 285, 642, 1554
BseBI CCWGG 2 cut(s) 1056, 1455
BseDI CCNNGG 5 cut(s) 508, 684, 905, 1092, 1423
BseGI GGATG 3 cut(s) 310, 388, 754
BseLI CCNNNNNNNGG 1 cut(s) 1890
BseMI GCAATG 5 cut(s) 158, 235, 285, 642, 1554
BseMII CTCAG 6 cut(s) 795, 1071, 1542, 1716, 1731, 1826
BseNI ACTGG 3 cut(s) 244, 376, 1702
BseRI GAGGAG 1 cut(s) 1161
BseYI CCCAGC 3 cut(s) 197, 1678, 1991
BsgI GTGCAG 2 cut(s) 105, 664
Bsh1236I CGCG 1 cut(s) 208
BshFI GGCC 9 cut(s) 236, 555, 665, 873, 902, 1480, 1613, 1707, 1845
BshNI GGYRCC 2 cut(s) 602, 1523
BshTI ACCGGT 1 cut(s) 1587
BsiHKAI GWGCWC 1 cut(s) 756
BsiHKCI CYCGRG 1 cut(s) 1883
BsiSI CCGG 6 cut(s) 167, 425, 1425, 1583, 1588, 1847
BslFI GGGAC 1 cut(s) 87
BslI CCNNNNNNNGG 1 cut(s) 1890
BsmAI GTCTC 1 cut(s) 538
BsmFI GGGAC 1 cut(s) 87
BsmI GAATGC 1 cut(s) 201
BsnI GGCC 9 cut(s) 236, 555, 665, 873, 902, 1480, 1613, 1707, 1845
BsoBI CYCGRG 1 cut(s) 1883
Bsp119I TTCGAA 1 cut(s) 832
Bsp1286I GDGCHC 1 cut(s) 756
Bsp143I GATC 7 cut(s) 351, 385, 1097, 1566, 1667, 1713, 1911
BspACI CCGC 3 cut(s) 105, 206, 1626
BspANI GGCC 9 cut(s) 236, 555, 665, 873, 902, 1480, 1613, 1707, 1845
BspCNI CTCAG 6 cut(s) 794, 1072, 1541, 1715, 1732, 1825
BspFNI CGCG 1 cut(s) 208
BspLI GGNNCC 4 cut(s) 604, 903, 1525, 1801
BspMI ACCTGC 3 cut(s) 680, 1277, 1603
BspOI GCTAGC 2 cut(s) 716, 1422
BspPI GGATC 1 cut(s) 1105
BspT104I TTCGAA 1 cut(s) 832
BspT107I GGYRCC 2 cut(s) 602, 1523
BsrDI GCAATG 5 cut(s) 158, 235, 285, 642, 1554
BsrFI RCCGGY 1 cut(s) 1587
BsrI ACTGG 3 cut(s) 244, 376, 1702
BssAI RCCGGY 1 cut(s) 1587
BssECI CCNNGG 5 cut(s) 508, 684, 905, 1092, 1423
BssMI GATC 7 cut(s) 351, 385, 1097, 1566, 1667, 1713, 1911
BssNAI GTATAC 1 cut(s) 2053
BssNI GRCGYC 1 cut(s) 603
BssT1I CCWWGG 3 cut(s) 508, 905, 1092
Bst1107I GTATAC 1 cut(s) 2053
Bst2UI CCWGG 2 cut(s) 1056, 1455
Bst4CI ACNGT 6 cut(s) 472, 743, 762, 919, 1339, 1831
BstACI GRCGYC 1 cut(s) 603
BstAPI GCANNNNNTGC 4 cut(s) 83, 653, 695, 1193
BstBI TTCGAA 1 cut(s) 832
BstC8I GCNNGC 6 cut(s) 184, 306, 607, 640, 714, 1420
BstDEI CTNAG 8 cut(s) 781, 1080, 1240, 1528, 1547, 1702, 1740, 1812
BstEII GGTNACC 1 cut(s) 1590
BstF5I GGATG 3 cut(s) 310, 388, 754
BstFNI CGCG 1 cut(s) 208
BstH2I RGCGCY 1 cut(s) 606
BstHHI GCGC 2 cut(s) 605, 1765
BstKTI GATC 7 cut(s) 354, 388, 1100, 1569, 1670, 1716, 1914
BstMAI GTCTC 1 cut(s) 538
BstMBI GATC 7 cut(s) 351, 385, 1097, 1566, 1667, 1713, 1911
BstMWI GCNNNNNNNGC 9 cut(s) 83, 644, 653, 662, 695, 815, 824, 1193, 1419
BstNI CCWGG 2 cut(s) 1056, 1455
BstNSI RCATGY 1 cut(s) 308
BstPI GGTNACC 1 cut(s) 1590
BstSCI CCNGG 5 cut(s) 1054, 1423, 1453, 1581, 1845
BstSFI CTRYAG 4 cut(s) 880, 1231, 1329, 1690
BstUI CGCG 1 cut(s) 208
BstX2I RGATCY 1 cut(s) 1097
BstXI CCANNNNNNTGG 1 cut(s) 800
BstYI RGATCY 1 cut(s) 1097
BstZ17I GTATAC 1 cut(s) 2053
BsuRI GGCC 9 cut(s) 236, 555, 665, 873, 902, 1480, 1613, 1707, 1845
BtsCI GGATG 3 cut(s) 310, 388, 754
BtsI GCAGTG 1 cut(s) 726
BveI ACCTGC 3 cut(s) 680, 1277, 1603
Cac8I GCNNGC 6 cut(s) 184, 306, 607, 640, 714, 1420
CaiI CAGNNNCTG 1 cut(s) 638
CfoI GCGC 2 cut(s) 605, 1765
Cfr10I RCCGGY 1 cut(s) 1587
Cfr13I GGNCC 7 cut(s) 872, 901, 1406, 1451, 1585, 1800, 1844
CpoI CGGWCCG 1 cut(s) 1585
Csp6I GTAC 6 cut(s) 80, 174, 465, 930, 1256, 1524
CspAI ACCGGT 1 cut(s) 1587
CspCI CAANNNNNGTGG 4 cut(s) 226, 261, 1950, 1985
CspI CGGWCCG 1 cut(s) 1585
CviAII CATG 8 cut(s) 58, 305, 557, 889, 1201, 1253, 1476, 1909
CviQI GTAC 6 cut(s) 80, 174, 465, 930, 1256, 1524
DdeI CTNAG 8 cut(s) 781, 1080, 1240, 1528, 1547, 1702, 1740, 1812
DinI GGCGCC 1 cut(s) 604
DpnI GATC 7 cut(s) 353, 387, 1099, 1568, 1669, 1715, 1913
DpnII GATC 7 cut(s) 351, 385, 1097, 1566, 1667, 1713, 1911
EaeI YGGCCR 3 cut(s) 234, 663, 1611
Eco130I CCWWGG 3 cut(s) 508, 905, 1092
Eco32I GATATC 2 cut(s) 378, 1444
Eco47I GGWCC 4 cut(s) 1406, 1451, 1585, 1800
Eco57I CTGAAG 1 cut(s) 1779
Eco88I CYCGRG 1 cut(s) 1883
Eco91I GGTNACC 1 cut(s) 1590
EcoO109I RGGNCCY 2 cut(s) 901, 1800
EcoO65I GGTNACC 1 cut(s) 1590
EcoRII CCWGG 2 cut(s) 1054, 1453
EcoRV GATATC 2 cut(s) 378, 1444
EcoT14I CCWWGG 3 cut(s) 508, 905, 1092
EgeI GGCGCC 1 cut(s) 604
EheI GGCGCC 1 cut(s) 604
ErhI CCWWGG 3 cut(s) 508, 905, 1092
FaeI CATG 8 cut(s) 61, 308, 560, 892, 1204, 1256, 1479, 1912
FaqI GGGAC 1 cut(s) 87
FatI CATG 8 cut(s) 57, 304, 556, 888, 1200, 1252, 1475, 1908
FauI CCCGC 1 cut(s) 112
FauNDI CATATG 1 cut(s) 1710
FbaI TGATCA 2 cut(s) 1566, 1713
FblI GTMKAC 1 cut(s) 2052
FokI GGATG 3 cut(s) 297, 375, 761
FspBI CTAG 7 cut(s) 74, 339, 573, 713, 1208, 1419, 1988
GlaI GCGC 2 cut(s) 604, 1764
GsaI CCCAGC 3 cut(s) 201, 1682, 1995
GsuI CTGGAG 1 cut(s) 366
HaeII RGCGCY 1 cut(s) 606
HaeIII GGCC 9 cut(s) 236, 555, 665, 873, 902, 1480, 1613, 1707, 1845
HapII CCGG 6 cut(s) 167, 425, 1425, 1583, 1588, 1847
HhaI GCGC 2 cut(s) 605, 1765
Hin1I GRCGYC 1 cut(s) 603
Hin1II CATG 8 cut(s) 61, 308, 560, 892, 1204, 1256, 1479, 1912
Hin6I GCGC 2 cut(s) 603, 1763
HinP1I GCGC 2 cut(s) 603, 1763
HincII GTYRAC 1 cut(s) 1948
HindII GTYRAC 1 cut(s) 1948
HindIII AAGCTT 1 cut(s) 485
HinfI GANTC 7 cut(s) 25, 910, 971, 998, 1204, 1749, 1918
HpaII CCGG 6 cut(s) 167, 425, 1425, 1583, 1588, 1847
HphI GGTGA 6 cut(s) 439, 557, 907, 1023, 1584, 1708
Hpy166II GTNNAC 7 cut(s) 217, 1213, 1577, 1732, 1906, 1948, 2053
Hpy188I TCNGA 5 cut(s) 156, 1741, 1815, 1870, 2038
Hpy8I GTNNAC 7 cut(s) 217, 1213, 1577, 1732, 1906, 1948, 2053
HpyAV CCTTC 6 cut(s) 401, 545, 1109, 1640, 1788, 1932
HpyCH4III ACNGT 6 cut(s) 472, 743, 762, 919, 1339, 1831
HpyF10VI GCNNNNNNNGC 9 cut(s) 83, 644, 653, 662, 695, 815, 824, 1193, 1419
HpyF3I CTNAG 8 cut(s) 781, 1080, 1240, 1528, 1547, 1702, 1740, 1812
Hsp92I GRCGYC 1 cut(s) 603
Hsp92II CATG 8 cut(s) 61, 308, 560, 892, 1204, 1256, 1479, 1912
HspAI GCGC 2 cut(s) 603, 1763
KasI GGCGCC 1 cut(s) 602
KpnI GGTACC 1 cut(s) 1527
Ksp22I TGATCA 2 cut(s) 1566, 1713
Kzo9I GATC 7 cut(s) 351, 385, 1097, 1566, 1667, 1713, 1911
LmnI GCTCC 3 cut(s) 824, 923, 1381
LweI GCATC 1 cut(s) 1237
MaeI CTAG 7 cut(s) 74, 339, 573, 713, 1208, 1419, 1988
MaeIII GTNAC 6 cut(s) 1038, 1498, 1590, 1750, 1831, 1915
MalI GATC 7 cut(s) 353, 387, 1099, 1568, 1669, 1715, 1913
MboI GATC 7 cut(s) 351, 385, 1097, 1566, 1667, 1713, 1911
MboII GAAGA 4 cut(s) 32, 560, 1167, 1184
MfeI CAATTG 1 cut(s) 1656
MflI RGATCY 1 cut(s) 1097
MhlI GDGCHC 1 cut(s) 756
MlsI TGGCCA 2 cut(s) 236, 665
MluCI AATT 7 cut(s) 502, 828, 840, 1018, 1428, 1656, 1816
MluNI TGGCCA 2 cut(s) 236, 665
Mly113I GGCGCC 1 cut(s) 603
MlyI GAGTC 2 cut(s) 1758, 1912
MmeI TCCRAC 1 cut(s) 901
Mox20I TGGCCA 2 cut(s) 236, 665
MscI TGGCCA 2 cut(s) 236, 665
MseI TTAA 2 cut(s) 1461, 1605
MslI CAYNNNNRTG 4 cut(s) 56, 798, 1548, 1913
Msp20I TGGCCA 2 cut(s) 236, 665
MspA1I CMGCKG 5 cut(s) 638, 852, 860, 1628, 1757
MspI CCGG 6 cut(s) 167, 425, 1425, 1583, 1588, 1847
MspR9I CCNGG 5 cut(s) 1056, 1425, 1455, 1583, 1847
MunI CAATTG 1 cut(s) 1656
Mva1269I GAATGC 1 cut(s) 201
MvaI CCWGG 2 cut(s) 1056, 1455
MvnI CGCG 1 cut(s) 208
MwoI GCNNNNNNNGC 9 cut(s) 83, 644, 653, 662, 695, 815, 824, 1193, 1419
NarI GGCGCC 1 cut(s) 603
NciI CCSGG 3 cut(s) 1425, 1583, 1847
NdeI CATATG 1 cut(s) 1710
NdeII GATC 7 cut(s) 351, 385, 1097, 1566, 1667, 1713, 1911
NheI GCTAGC 2 cut(s) 712, 1418
NlaIII CATG 8 cut(s) 61, 308, 560, 892, 1204, 1256, 1479, 1912
NlaIV GGNNCC 4 cut(s) 604, 903, 1525, 1801
NmuCI GTSAC 5 cut(s) 1498, 1590, 1750, 1831, 1915
NspI RCATGY 1 cut(s) 308
NspV TTCGAA 1 cut(s) 832
OliI CACNNNNGTG 1 cut(s) 1548
PaeI GCATGC 1 cut(s) 308
PaqCI CACCTGC 1 cut(s) 1603
PctI GAATGC 1 cut(s) 201
PfeI GAWTC 5 cut(s) 25, 910, 971, 998, 1204
PinAI ACCGGT 1 cut(s) 1587
PleI GAGTC 2 cut(s) 1757, 1912
PluTI GGCGCC 1 cut(s) 606
PpsI GAGTC 2 cut(s) 1757, 1912
PpuMI RGGWCCY 1 cut(s) 1800
Psp5II RGGWCCY 1 cut(s) 1800
Psp6I CCWGG 2 cut(s) 1054, 1453
PspEI GGTNACC 1 cut(s) 1590
PspFI CCCAGC 3 cut(s) 197, 1678, 1991
PspGI CCWGG 2 cut(s) 1054, 1453
PspN4I GGNNCC 4 cut(s) 604, 903, 1525, 1801
PspPI GGNCC 7 cut(s) 872, 901, 1406, 1451, 1585, 1800, 1844
PspPPI RGGWCCY 1 cut(s) 1800
PstNI CAGNNNCTG 1 cut(s) 638
PsuI RGATCY 1 cut(s) 1097
PvuII CAGCTG 4 cut(s) 638, 852, 860, 1757
RsaI GTAC 6 cut(s) 81, 175, 466, 931, 1257, 1525
RsaNI GTAC 6 cut(s) 80, 174, 465, 930, 1256, 1524
RseI CAYNNNNRTG 4 cut(s) 56, 798, 1548, 1913
Rsr2I CGGWCCG 1 cut(s) 1585
RsrII CGGWCCG 1 cut(s) 1585
SaqAI TTAA 2 cut(s) 1461, 1605
Sau3AI GATC 7 cut(s) 351, 385, 1097, 1566, 1667, 1713, 1911
Sau96I GGNCC 7 cut(s) 872, 901, 1406, 1451, 1585, 1800, 1844
ScaI AGTACT 2 cut(s) 81, 175
SchI GAGTC 2 cut(s) 1758, 1912
ScrFI CCNGG 5 cut(s) 1056, 1425, 1455, 1583, 1847
SduI GDGCHC 1 cut(s) 756
SfaNI GCATC 1 cut(s) 1237
SfcI CTRYAG 4 cut(s) 880, 1231, 1329, 1690
SfoI GGCGCC 1 cut(s) 604
SfuI TTCGAA 1 cut(s) 832
SinI GGWCC 4 cut(s) 1406, 1451, 1585, 1800
SmiMI CAYNNNNRTG 4 cut(s) 56, 798, 1548, 1913
SmlI CTYRAG 1 cut(s) 132
SmoI CTYRAG 1 cut(s) 132
SpeI ACTAGT 1 cut(s) 338
SphI GCATGC 1 cut(s) 308
Sse9I AATT 7 cut(s) 502, 828, 840, 1018, 1428, 1656, 1816
SsiI CCGC 3 cut(s) 105, 206, 1626
SspDI GGCGCC 1 cut(s) 602
SspI AATATT 1 cut(s) 983
SspMI CTAG 7 cut(s) 74, 339, 573, 713, 1208, 1419, 1988
StyD4I CCNGG 5 cut(s) 1054, 1423, 1453, 1581, 1845
StyI CCWWGG 3 cut(s) 508, 905, 1092
TaaI ACNGT 6 cut(s) 472, 743, 762, 919, 1339, 1831
TaqI TCGA 3 cut(s) 28, 832, 1644
TaqII GACCGA 1 cut(s) 1423
TasI AATT 7 cut(s) 502, 828, 840, 1018, 1428, 1656, 1816
TatI WGTACW 3 cut(s) 79, 173, 1255
TfiI GAWTC 5 cut(s) 25, 910, 971, 998, 1204
Tru1I TTAA 2 cut(s) 1461, 1605
Tru9I TTAA 2 cut(s) 1461, 1605
TseFI GTSAC 5 cut(s) 1498, 1590, 1750, 1831, 1915
Tsp45I GTSAC 5 cut(s) 1498, 1590, 1750, 1831, 1915
TspDTI ATGAA 7 cut(s) 40, 46, 53, 105, 420, 1770, 1995
VpaK11BI GGWCC 4 cut(s) 1406, 1451, 1585, 1800
XapI RAATTY 4 cut(s) 502, 840, 1018, 1428
XceI RCATGY 1 cut(s) 308
XmiI GTMKAC 1 cut(s) 2052
XspI CTAG 7 cut(s) 74, 339, 573, 713, 1208, 1419, 1988
ZrmI AGTACT 2 cut(s) 81, 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.