MD06G1068800.v1.1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000302
Physical Location & Seq
Forward (+)
16580125 .. 16581957
1833 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1068800.v1.1.491

Sequence Viewer

Length: 1746 bp
ATGCTCCTATCAGTGTTGGATACTGGGATTGGTCCTGATCATCCTTCGTTTAGCGATGAAGGAGTACCACCTCCTCCAGCTAAATGGAAAGGCAAGTGCGACTTCAATGGACCGGTGTGCAATAACAAGCTTGTAGGTGCACGAAACTTCCGAAGCGGACAATCTACTGGAGGCCCTCCAGTTGACGATGAAGGCCATGGCACCCACACATCCAGCACAGCTGCAGGAAATTTTGTGAAAGGTGCCAATGCGTTTGGAATGGCCAATGGCACAGCAACTGGCATGGCACCTTATGCTCATATGGCAATGTACAAGGTCTGTGGTGAGGCGGGTTGTTCTGATGCAGACATTTTAGCTGCTATGGATACTGCTGTCGAAGATGGCGTTGACATCCTCTCCCTCTCACTTGGCGGAAATTCTGGTCCTGACTACGAGTCTTTATCCAATGAGGCCCTATGGATACTCACAGTTGGAGCAAGCACCATTGACAGAAGCAAAAGAGCAACAGCGCTCCTTGGAAATCATGGAGAATTTGATGACGTATCTTTGAACCAGCCTAAAGATTTTAATTCGACATTGTTGCCTCTTGTTTATCCAGGTGTGAACGGCGATCAGTCATTATCTTTATGTGCTCCAGGATCCCTTGGAAATGTTGAAGGGAAAATAGTGTTGTGCGAGGGAGGAAGAGGAAGAGTTGCCAAAGGGGAAGAACTGAAAAGAGCTGGTGGTGCTGCCATGATTTTGGCAAACCTAGAGGTTGGTGGCTACACCGTCTTAGCTGACGCTCATGTTCTTCCTGCGACACATGTGAGTTATGCTGCAGGGTTGATGATCAAAGCCTACATAAACTCAACCGCAACACCTCAAGCCACAATCTCGTTCAAAGGCACTGTCATTAAAGATCCTCTAGCTCCCCAAGTCGCTTTCTTTTCATCAAAAGGACCAAGCATTGCAAGCCCTGGAATTTTGAAGCTTGACATCATTGGACCTGGTGTTAGCATCCTAGCGGCATGGCCTGTTTCTGTGGACAATGCCACACTTCCCAATCCTAAGGCAACATTTAACATGGTTTCAGGTACTTCGATGTCATGCCCTCACTTAAGTGGCATTGCAGCCTTGCTCAAGAGTTCCCACCCAGACTGGTCACCGGCTGCCATTAAGTCTGCAATCATGACAACGACAAAAGTAAATAACCTTGGAAGACAGTCCATAGTTGATGAAACACTTAAACTAGCAGACATCTTTGCCACTGGTGCAGGCCATGTTAACCCTTCAAACGCAATTGACCCAGGGCTCATCTATGACATAAATCCAGCAGATTACATTCCTTATATGTGCGGTTTGAATTACACAAACGAACAGATACAGGTTGTCACCCAACAAACAGTGAACTGCTCGGAAGTGGGAACCATCCCAGAAGCACAACTAAACTATCCTTCATTTTCTATTATAATAGGGTCATCTGATGAGTCTAAGTCTCAGGACTATACAAGGACGGTGAAGAATGTTGGGCCGGCTAGTTCAACTTACAAGCTGGATCTTAATGTGCCACATAAAATGGGCATGAGTGTGAACCCTGAGGTGCTAACATTCACAGAGGTTAACCAGGAGATTACATTCAGTGTGGAGTTTATTGCGCAAGAAGGGACTGGGAAGGATGGTGTACTGTTTGATCGGGGGTATCTGAAATGGATTTCTGATAAGCACTATGTTACTAGCCCCATCTCTGTGATCTTTGACACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

582

Amino Acids

60.85

Weight (kDa)

5.07

Isoelectric Point (pI)

29.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 3 - 395 6.1e-38 Subtilase family
PA PF02225 195 - 277 3.7e-11 PA domain
fn3_6 PF17766 475 - 577 3.3e-24 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000302)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01091 FvH4_5g01093 FvH4_5g01120 FvH4_7g08400
malus_domestica MD00G1069300.v1.1 MD00G1069500.v1.1 MD04G1035800.v1.1 MD04G1071200.v1.1 MD04G1071500.v1.1 MD04G1071800.v1.1 MD04G1071900.v1.1 MD04G1072000.v1.1 MD04G1072200.v1.1 MD04G1072600.v1.1 MD04G1072700.v1.1 MD06G1068800.v1.1
prunus_persica Prupe.5G085000_v2.0.a1 Prupe.5G085100_v2.0.a1 Prupe.5G085200_v2.0.a1 Prupe.5G085300_v2.0.a1 Prupe.5G085400_v2.0.a1 Prupe.5G085500_v2.0.a1 Prupe.5G085600_v2.0.a1 Prupe.5G085700_v2.0.a1 Prupe.5G085800_v2.0.a1 Prupe.5G086100_v2.0.a1
pyrus_communis pycom04g02960 pycom04g06430 pycom04g06590
rosa_chinensis RchiOBHm_Chr0c20g0500201 RchiOBHm_Chr0c20g0500211 RchiOBHm_Chr6g0266941 RchiOBHm_Chr7g0200021 RchiOBHm_Chr7g0200051 RchiOBHm_Chr7g0200061 RchiOBHm_Chr7g0200121 RchiOBHm_Chr7g0200141 RchiOBHm_Chr7g0200151 RchiOBHm_Chr7g0200181 RchiOBHm_Chr7g0200191
rosa_laevigata RLG00000003743 RLG00000003744 RLG00000003745 RLG00000003746 RLG00000003747 RLG00000003748 RLG00000003749 RLG00000003750 RLG00000028998 RLG00000028999 RLG00000029001
rosa_multiflora Rmu_co8342527.1_g000001 Rmu_co8411041.1_g000001 Rmu_sc0003357.1_g000004 Rmu_sc0003357.1_g000005 Rmu_sc0003357.1_g000007 Rmu_sc0006398.1_g000001 Rmu_sc0006398.1_g000003 Rmu_sc0006398.1_g000007 Rmu_sc0006398.1_g000008 Rmu_sc0006398.1_g000009 Rmu_sc0006685.1_g000001 Rmu_sc0010281.1_g000004 Rmu_sc0020375.1_g000001 Rmu_ssc0000486.1_g000017
rosa_roxburghii Rroxscaffold_3G00256220 Rroxscaffold_3G00256230 Rroxscaffold_3G00256240 Rroxscaffold_3G00256250 Rroxscaffold_3G00256260 Rroxscaffold_3G00256270 Rroxscaffold_3G00256280 Rroxscaffold_4G00311060
rosa_rugosa Rorug01G0165100 Rorug01G0165100 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058000 Rorug07G0058100 Rorug07G0058100 Rorug07G0058200
rosa_samantha Rh1AG180000 Rh1CG167000 Rh1DG180300 Rh6AG149600 Rh6BG150600 Rh6CG145600 Rh7AG183300 Rh7AG183400 Rh7AG183800 Rh7AG184000 Rh7CG193000 Rh7CG193100 Rh7CG193200 Rh7CG193500 Rh7CG193600 Rh7CG193700 Rh7CG193800 Rh7CG194000 Rh7DG186300 Rh7DG186400 Rh7DG186500 Rh7DG186700 Rh7DG186800 Rh7DG187400 Rh7DG187500 Rh7DG187600 Rh7DG187800 Rh7DG187900 Rh7DG188000
rosa_wichuraiana Rw1G014970 Rw6G012950 Rw7G016050 Rw7G016060 Rw7G016070 Rw7G016080 Rw7G016090 Rw7G016100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1451
Acc16I TGCGCA 1 cut(s) 1638
AccB1I GGYRCC 3 cut(s) 200, 242, 286
AciI CCGC 6 cut(s) 156, 329, 411, 855, 1007, 1338
AclWI GGATC 4 cut(s) 633, 646, 896, 1545
AcoI YGGCCR 1 cut(s) 261
AcsI RAATTY 4 cut(s) 229, 415, 530, 963
AfaI GTAC 4 cut(s) 66, 311, 1078, 1665
AfeI AGCGCT 1 cut(s) 510
AflII CTTAAG 1 cut(s) 1099
AflIII ACRYGT 1 cut(s) 805
AgeI ACCGGT 1 cut(s) 112
AgsI TTSAA 8 cut(s) 106, 550, 656, 883, 970, 1275, 1345, 1524
AhdI GACNNNNNGTC 1 cut(s) 433
AjnI CCWGG 6 cut(s) 595, 634, 958, 988, 1288, 1605
AleI CACNNNNGTG 1 cut(s) 1101
AluBI AGCT 9 cut(s) 80, 130, 221, 356, 722, 779, 911, 973, 1534
AluI AGCT 9 cut(s) 80, 130, 221, 356, 722, 779, 911, 973, 1534
Alw21I GWGCWC 2 cut(s) 142, 634
Alw26I GTCTC 1 cut(s) 1482
Alw44I GTGCAC 1 cut(s) 138
AlwI GGATC 4 cut(s) 633, 646, 896, 1545
AlwNI CAGNNNCTG 1 cut(s) 278
Aor51HI AGCGCT 1 cut(s) 510
AoxI GGCC 7 cut(s) 172, 193, 261, 450, 1013, 1258, 1511
ApaLI GTGCAC 1 cut(s) 138
ApeKI GCWGC 6 cut(s) 221, 356, 731, 818, 1112, 1151
ApoI RAATTY 4 cut(s) 229, 415, 530, 963
ArsI GACNNNNNNTTYG 2 cut(s) 876, 908
AsiGI ACCGGT 1 cut(s) 112
AspLEI GCGC 2 cut(s) 511, 1639
AspS9I GGNCC 8 cut(s) 32, 110, 173, 422, 451, 941, 986, 1511
AsuHPI GGTGA 4 cut(s) 335, 1137, 1366, 1510
AvaII GGWCC 5 cut(s) 32, 110, 422, 941, 986
AxyI CCTNAGG 2 cut(s) 1050, 1578
BaeGI GKGCMC 1 cut(s) 142
BaeI ACNNNNGTAYC 2 cut(s) 357, 390
BalI TGGCCA 1 cut(s) 263
BamHI GGATCC 1 cut(s) 638
BanI GGYRCC 3 cut(s) 200, 242, 286
BanII GRGCYC 1 cut(s) 1296
BbsI GAAGAC 1 cut(s) 1207
Bbv12I GWGCWC 2 cut(s) 142, 634
BbvI GCAGC 6 cut(s) 208, 343, 718, 805, 1124, 1138
BccI CCATC 4 cut(s) 374, 1418, 1652, 1730
BceAI ACGGC 1 cut(s) 622
BcgI CGANNNNNNTGC 2 cut(s) 562, 596
BciT130I CCWGG 6 cut(s) 597, 636, 960, 990, 1290, 1607
BciVI GTATCC 3 cut(s) 13, 358, 453
BclI TGATCA 2 cut(s) 37, 831
BcoDI GTCTC 1 cut(s) 1482
BfaI CTAG 6 cut(s) 752, 908, 1004, 1232, 1518, 1716
BfmI CTRYAG 2 cut(s) 222, 819
BfoI RGCGCY 1 cut(s) 512
BfrI CTTAAG 1 cut(s) 1099
BfuI GTATCC 3 cut(s) 13, 358, 453
BisI GCNGC 7 cut(s) 222, 357, 732, 819, 1008, 1113, 1152
BlsI GCNGC 7 cut(s) 223, 358, 733, 820, 1009, 1114, 1153
Bme1390I CCNGG 6 cut(s) 597, 636, 960, 990, 1290, 1607
Bme18I GGWCC 5 cut(s) 32, 110, 422, 941, 986
BmeRI GACNNNNNGTC 1 cut(s) 433
BmgT120I GGNCC 8 cut(s) 32, 110, 173, 422, 451, 941, 986, 1511
BmiI GGNNCC 5 cut(s) 202, 244, 288, 640, 1408
BmrFI CCNGG 6 cut(s) 597, 636, 960, 990, 1290, 1607
BmrI ACTGGG 2 cut(s) 33, 1659
BmsI GCATC 2 cut(s) 331, 1008
BmuI ACTGGG 2 cut(s) 33, 1659
BpiI GAAGAC 1 cut(s) 1207
BpmI CTGGAG 4 cut(s) 60, 162, 189, 618
BpuEI CTTGAG 2 cut(s) 849, 1106
BsaJI CCNNGG 7 cut(s) 196, 514, 643, 958, 1195, 1288, 1289
BsaWI WCCGGW 1 cut(s) 112
BsaXI ACNNNNNCTCC 2 cut(s) 380, 410
Bse118I RCCGGY 3 cut(s) 112, 1147, 1513
Bse1I ACTGG 7 cut(s) 28, 172, 179, 283, 1145, 1255, 1654
Bse21I CCTNAGG 2 cut(s) 1050, 1578
Bse3DI GCAATG 3 cut(s) 312, 948, 1107
BseBI CCWGG 6 cut(s) 597, 636, 960, 990, 1290, 1607
BseDI CCNNGG 7 cut(s) 196, 514, 643, 958, 1195, 1288, 1289
BseGI GGATG 6 cut(s) 40, 209, 390, 999, 1410, 1663
BseMI GCAATG 3 cut(s) 312, 948, 1107
BseMII CTCAG 2 cut(s) 1493, 1569
BseNI ACTGG 7 cut(s) 28, 172, 179, 283, 1145, 1255, 1654
BseRI GAGGAG 1 cut(s) 63
BseSI GKGCMC 1 cut(s) 142
BseXI GCAGC 6 cut(s) 208, 343, 718, 805, 1124, 1138
BsgI GTGCAG 1 cut(s) 1275
BshFI GGCC 7 cut(s) 174, 195, 263, 452, 1015, 1260, 1513
BshNI GGYRCC 3 cut(s) 200, 242, 286
BshTI ACCGGT 1 cut(s) 112
BsiHKAI GWGCWC 2 cut(s) 142, 634
BsiSI CCGG 3 cut(s) 113, 1148, 1514
BslFI GGGAC 1 cut(s) 1660
BsmAI GTCTC 1 cut(s) 1482
BsmFI GGGAC 1 cut(s) 1660
BsnI GGCC 7 cut(s) 174, 195, 263, 452, 1015, 1260, 1513
Bsp1286I GDGCHC 3 cut(s) 142, 634, 1296
Bsp1407I TGTACA 1 cut(s) 309
Bsp143I GATC 8 cut(s) 37, 610, 638, 831, 901, 1537, 1672, 1731
Bsp19I CCATGG 1 cut(s) 196
BspACI CCGC 6 cut(s) 156, 329, 411, 855, 1007, 1338
BspANI GGCC 7 cut(s) 174, 195, 263, 452, 1015, 1260, 1513
BspCNI CTCAG 2 cut(s) 1492, 1570
BspHI TCATGA 1 cut(s) 1170
BspLI GGNNCC 5 cut(s) 202, 244, 288, 640, 1408
BspMAI CTGCAG 2 cut(s) 226, 823
BspPI GGATC 4 cut(s) 633, 646, 896, 1545
BspT107I GGYRCC 3 cut(s) 200, 242, 286
BspTI CTTAAG 1 cut(s) 1099
BsrDI GCAATG 3 cut(s) 312, 948, 1107
BsrFI RCCGGY 3 cut(s) 112, 1147, 1513
BsrGI TGTACA 1 cut(s) 309
BsrI ACTGG 7 cut(s) 28, 172, 179, 283, 1145, 1255, 1654
BssAI RCCGGY 3 cut(s) 112, 1147, 1513
BssECI CCNNGG 7 cut(s) 196, 514, 643, 958, 1195, 1288, 1289
BssMI GATC 8 cut(s) 37, 610, 638, 831, 901, 1537, 1672, 1731
BssT1I CCWWGG 4 cut(s) 196, 514, 643, 1195
Bst2UI CCWGG 6 cut(s) 597, 636, 960, 990, 1290, 1607
Bst4CI ACNGT 7 cut(s) 469, 772, 892, 1206, 1387, 1498, 1668
Bst6I CTCTTC 2 cut(s) 679, 685
BstAFI CTTAAG 1 cut(s) 1099
BstAPI GCANNNNNTGC 1 cut(s) 293
BstAUI TGTACA 1 cut(s) 309
BstC8I GCNNGC 4 cut(s) 478, 955, 1258, 1515
BstDEI CTNAG 5 cut(s) 775, 1050, 1473, 1479, 1578
BstDSI CCRYGG 1 cut(s) 196
BstEII GGTNACC 1 cut(s) 1143
BstF5I GGATG 6 cut(s) 40, 209, 390, 999, 1410, 1663
BstH2I RGCGCY 1 cut(s) 512
BstHHI GCGC 2 cut(s) 511, 1639
BstKTI GATC 8 cut(s) 40, 613, 641, 834, 904, 1540, 1675, 1734
BstMAI GTCTC 1 cut(s) 1482
BstMBI GATC 8 cut(s) 37, 610, 638, 831, 901, 1537, 1672, 1731
BstMWI GCNNNNNNNGC 5 cut(s) 293, 302, 728, 954, 1253
BstNI CCWGG 6 cut(s) 597, 636, 960, 990, 1290, 1607
BstNSI RCATGY 1 cut(s) 809
BstPI GGTNACC 1 cut(s) 1143
BstSCI CCNGG 6 cut(s) 595, 634, 958, 988, 1288, 1605
BstSFI CTRYAG 2 cut(s) 222, 819
BstSLI GKGCMC 1 cut(s) 142
BstV1I GCAGC 6 cut(s) 208, 343, 718, 805, 1124, 1138
BstV2I GAAGAC 1 cut(s) 1207
BstX2I RGATCY 3 cut(s) 638, 901, 1537
BstXI CCANNNNNNTGG 2 cut(s) 84, 742
BstYI RGATCY 3 cut(s) 638, 901, 1537
Bsu36I CCTNAGG 2 cut(s) 1050, 1578
BsuI GTATCC 3 cut(s) 13, 358, 453
BsuRI GGCC 7 cut(s) 174, 195, 263, 452, 1015, 1260, 1513
BtgI CCRYGG 1 cut(s) 196
BtgZI GCGATG 1 cut(s) 69
BtsCI GGATG 6 cut(s) 40, 209, 390, 999, 1410, 1663
BtsIMutI CAGTG 5 cut(s) 18, 888, 1248, 1392, 1627
Cac8I GCNNGC 4 cut(s) 478, 955, 1258, 1515
CaiI CAGNNNCTG 1 cut(s) 278
CciI TCATGA 1 cut(s) 1170
CfoI GCGC 2 cut(s) 511, 1639
Cfr10I RCCGGY 3 cut(s) 112, 1147, 1513
Cfr13I GGNCC 8 cut(s) 32, 110, 173, 422, 451, 941, 986, 1511
CseI GACGC 1 cut(s) 791
CsiI ACCWGGT 1 cut(s) 988
Csp6I GTAC 4 cut(s) 65, 310, 1077, 1664
CspAI ACCGGT 1 cut(s) 112
CspCI CAANNNNNGTGG 2 cut(s) 301, 336
CviQI GTAC 4 cut(s) 65, 310, 1077, 1664
DdeI CTNAG 5 cut(s) 775, 1050, 1473, 1479, 1578
DpnI GATC 8 cut(s) 39, 612, 640, 833, 903, 1539, 1674, 1733
DpnII GATC 8 cut(s) 37, 610, 638, 831, 901, 1537, 1672, 1731
DriI GACNNNNNGTC 1 cut(s) 433
EaeI YGGCCR 1 cut(s) 261
Eam1104I CTCTTC 2 cut(s) 679, 685
Eam1105I GACNNNNNGTC 1 cut(s) 433
EarI CTCTTC 2 cut(s) 679, 685
EciI GGCGGA 1 cut(s) 426
Eco130I CCWWGG 4 cut(s) 196, 514, 643, 1195
Eco24I GRGCYC 1 cut(s) 1296
Eco47I GGWCC 5 cut(s) 32, 110, 422, 941, 986
Eco47III AGCGCT 1 cut(s) 510
Eco81I CCTNAGG 2 cut(s) 1050, 1578
Eco91I GGTNACC 1 cut(s) 1143
EcoO109I RGGNCCY 2 cut(s) 173, 451
EcoO65I GGTNACC 1 cut(s) 1143
EcoRII CCWGG 6 cut(s) 595, 634, 958, 988, 1288, 1605
EcoT14I CCWWGG 4 cut(s) 196, 514, 643, 1195
EcoT38I GRGCYC 1 cut(s) 1296
ErhI CCWWGG 4 cut(s) 196, 514, 643, 1195
FalI AAGNNNNNCTT 2 cut(s) 86, 118
FaqI GGGAC 1 cut(s) 1660
FauI CCCGC 1 cut(s) 322
FauNDI CATATG 1 cut(s) 300
FbaI TGATCA 2 cut(s) 37, 831
Fnu4HI GCNGC 7 cut(s) 222, 357, 732, 819, 1008, 1113, 1152
FokI GGATG 6 cut(s) 27, 196, 377, 986, 1397, 1670
FriOI GRGCYC 1 cut(s) 1296
Fsp4HI GCNGC 7 cut(s) 222, 357, 732, 819, 1008, 1113, 1152
FspBI CTAG 6 cut(s) 752, 908, 1004, 1232, 1518, 1716
FspI TGCGCA 1 cut(s) 1638
GlaI GCGC 2 cut(s) 510, 1638
GluI GCNGC 7 cut(s) 222, 357, 732, 819, 1008, 1113, 1152
GsuI CTGGAG 4 cut(s) 60, 162, 189, 618
HaeII RGCGCY 1 cut(s) 512
HaeIII GGCC 7 cut(s) 174, 195, 263, 452, 1015, 1260, 1513
HapII CCGG 3 cut(s) 113, 1148, 1514
HgaI GACGC 1 cut(s) 791
HhaI GCGC 2 cut(s) 511, 1639
Hin6I GCGC 2 cut(s) 509, 1637
HinP1I GCGC 2 cut(s) 509, 1637
HincII GTYRAC 4 cut(s) 184, 388, 1267, 1603
HindII GTYRAC 4 cut(s) 184, 388, 1267, 1603
HindIII AAGCTT 2 cut(s) 128, 971
HinfI GANTC 2 cut(s) 434, 1469
HpaI GTTAAC 2 cut(s) 1267, 1603
HpaII CCGG 3 cut(s) 113, 1148, 1514
HphI GGTGA 4 cut(s) 335, 1137, 1366, 1510
Hpy188I TCNGA 6 cut(s) 152, 340, 1399, 1465, 1686, 1699
Hpy188III TCNNGA 5 cut(s) 35, 425, 1123, 1171, 1481
HpyAV CCTTC 8 cut(s) 53, 54, 185, 650, 1281, 1446, 1637, 1648
HpyCH4III ACNGT 7 cut(s) 469, 772, 892, 1206, 1387, 1498, 1668
HpyCH4IV ACGT 1 cut(s) 540
HpyCH4V TGCA 9 cut(s) 120, 140, 224, 344, 821, 953, 1112, 1166, 1256
HpyF10VI GCNNNNNNNGC 5 cut(s) 293, 302, 728, 954, 1253
HpyF3I CTNAG 5 cut(s) 775, 1050, 1473, 1479, 1578
HpySE526I ACGT 1 cut(s) 540
HspAI GCGC 2 cut(s) 509, 1637
KroI GCCGGC 1 cut(s) 1513
KroNI GCCGGC 1 cut(s) 1515
Ksp22I TGATCA 2 cut(s) 37, 831
KspAI GTTAAC 2 cut(s) 1267, 1603
Kzo9I GATC 8 cut(s) 37, 610, 638, 831, 901, 1537, 1672, 1731
LmnI GCTCC 5 cut(s) 9, 473, 516, 637, 916
Lsp1109I GCAGC 6 cut(s) 208, 343, 718, 805, 1124, 1138
LweI GCATC 2 cut(s) 331, 1008
MabI ACCWGGT 1 cut(s) 988
MaeI CTAG 6 cut(s) 752, 908, 1004, 1232, 1518, 1716
MaeII ACGT 1 cut(s) 540
MaeIII GTNAC 3 cut(s) 1143, 1372, 1711
MalI GATC 8 cut(s) 39, 612, 640, 833, 903, 1539, 1674, 1733
MboI GATC 8 cut(s) 37, 610, 638, 831, 901, 1537, 1672, 1731
MboII GAAGA 7 cut(s) 389, 696, 702, 719, 785, 1212, 1513
MfeI CAATTG 1 cut(s) 1281
MflI RGATCY 3 cut(s) 638, 901, 1537
MhlI GDGCHC 3 cut(s) 142, 634, 1296
MlsI TGGCCA 1 cut(s) 263
MluCI AATT 7 cut(s) 229, 415, 530, 568, 963, 1281, 1345
MluNI TGGCCA 1 cut(s) 263
MlyI GAGTC 2 cut(s) 443, 1478
MmeI TCCRAC 1 cut(s) 451
Mox20I TGGCCA 1 cut(s) 263
MroNI GCCGGC 1 cut(s) 1513
MscI TGGCCA 1 cut(s) 263
MseI TTAA 9 cut(s) 567, 897, 1062, 1100, 1158, 1227, 1266, 1542, 1602
MslI CAYNNNNRTG 3 cut(s) 1101, 1568, 1727
Msp20I TGGCCA 1 cut(s) 263
MspA1I CMGCKG 1 cut(s) 221
MspCI CTTAAG 1 cut(s) 1099
MspI CCGG 3 cut(s) 113, 1148, 1514
MspR9I CCNGG 6 cut(s) 597, 636, 960, 990, 1290, 1607
MunI CAATTG 1 cut(s) 1281
MvaI CCWGG 6 cut(s) 597, 636, 960, 990, 1290, 1607
MwoI GCNNNNNNNGC 5 cut(s) 293, 302, 728, 954, 1253
NaeI GCCGGC 1 cut(s) 1515
NcoI CCATGG 1 cut(s) 196
NdeI CATATG 1 cut(s) 300
NdeII GATC 8 cut(s) 37, 610, 638, 831, 901, 1537, 1672, 1731
NgoMIV GCCGGC 1 cut(s) 1513
NlaIV GGNNCC 5 cut(s) 202, 244, 288, 640, 1408
NmuCI GTSAC 2 cut(s) 1143, 1372
NsbI TGCGCA 1 cut(s) 1638
NspI RCATGY 1 cut(s) 809
OliI CACNNNNGTG 1 cut(s) 1101
PagI TCATGA 1 cut(s) 1170
PasI CCCWGGG 1 cut(s) 1289
PciI ACATGT 1 cut(s) 805
PcsI WCGNNNNNNNCGW 2 cut(s) 148, 381
PdiI GCCGGC 1 cut(s) 1515
PfoI TCCNGGA 1 cut(s) 634
PinAI ACCGGT 1 cut(s) 112
PkrI GCNGC 7 cut(s) 223, 358, 733, 820, 1009, 1114, 1153
PleI GAGTC 2 cut(s) 442, 1477
PpsI GAGTC 2 cut(s) 442, 1477
PscI ACATGT 1 cut(s) 805
PsiI TTATAA 1 cut(s) 1451
Psp6I CCWGG 6 cut(s) 595, 634, 958, 988, 1288, 1605
PspEI GGTNACC 1 cut(s) 1143
PspGI CCWGG 6 cut(s) 595, 634, 958, 988, 1288, 1605
PspN4I GGNNCC 5 cut(s) 202, 244, 288, 640, 1408
PspPI GGNCC 8 cut(s) 32, 110, 173, 422, 451, 941, 986, 1511
PstI CTGCAG 2 cut(s) 226, 823
PstNI CAGNNNCTG 1 cut(s) 278
PsuI RGATCY 3 cut(s) 638, 901, 1537
PvuII CAGCTG 1 cut(s) 221
RsaI GTAC 4 cut(s) 66, 311, 1078, 1665
RsaNI GTAC 4 cut(s) 65, 310, 1077, 1664
RseI CAYNNNNRTG 3 cut(s) 1101, 1568, 1727
SaqAI TTAA 9 cut(s) 567, 897, 1062, 1100, 1158, 1227, 1266, 1542, 1602
SatI GCNGC 7 cut(s) 222, 357, 732, 819, 1008, 1113, 1152
Sau3AI GATC 8 cut(s) 37, 610, 638, 831, 901, 1537, 1672, 1731
Sau96I GGNCC 8 cut(s) 32, 110, 173, 422, 451, 941, 986, 1511
SchI GAGTC 2 cut(s) 443, 1478
ScrFI CCNGG 6 cut(s) 597, 636, 960, 990, 1290, 1607
SduI GDGCHC 3 cut(s) 142, 634, 1296
SexAI ACCWGGT 1 cut(s) 988
SfaNI GCATC 2 cut(s) 331, 1008
SfcI CTRYAG 2 cut(s) 222, 819
SinI GGWCC 5 cut(s) 32, 110, 422, 941, 986
SmiMI CAYNNNNRTG 3 cut(s) 1101, 1568, 1727
SmlI CTYRAG 3 cut(s) 864, 1099, 1121
SmoI CTYRAG 3 cut(s) 864, 1099, 1121
Sse9I AATT 7 cut(s) 229, 415, 530, 568, 963, 1281, 1345
SsiI CCGC 6 cut(s) 156, 329, 411, 855, 1007, 1338
SspMI CTAG 6 cut(s) 752, 908, 1004, 1232, 1518, 1716
StyD4I CCNGG 6 cut(s) 595, 634, 958, 988, 1288, 1605
StyI CCWWGG 4 cut(s) 196, 514, 643, 1195
TaaI ACNGT 7 cut(s) 469, 772, 892, 1206, 1387, 1498, 1668
TaiI ACGT 1 cut(s) 543
TaqI TCGA 3 cut(s) 375, 572, 1082
TasI AATT 7 cut(s) 229, 415, 530, 568, 963, 1281, 1345
TatI WGTACW 2 cut(s) 309, 1663
TauI GCSGC 1 cut(s) 1010
Tru1I TTAA 9 cut(s) 567, 897, 1062, 1100, 1158, 1227, 1266, 1542, 1602
Tru9I TTAA 9 cut(s) 567, 897, 1062, 1100, 1158, 1227, 1266, 1542, 1602
TscAI CASTG 5 cut(s) 18, 895, 1255, 1392, 1627
TseFI GTSAC 2 cut(s) 1143, 1372
TseI GCWGC 6 cut(s) 221, 356, 731, 818, 1112, 1151
Tsp45I GTSAC 2 cut(s) 1143, 1372
TspDTI ATGAA 5 cut(s) 72, 204, 921, 1233, 1428
TspRI CASTG 5 cut(s) 18, 895, 1255, 1392, 1627
Vha464I CTTAAG 1 cut(s) 1099
VneI GTGCAC 1 cut(s) 138
VpaK11BI GGWCC 5 cut(s) 32, 110, 422, 941, 986
XapI RAATTY 4 cut(s) 229, 415, 530, 963
XceI RCATGY 1 cut(s) 809
XspI CTAG 6 cut(s) 752, 908, 1004, 1232, 1518, 1716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.